CN114787193A - 抗聚合酶的单克隆抗体及其应用 - Google Patents

抗聚合酶的单克隆抗体及其应用 Download PDF

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CN114787193A
CN114787193A CN201980102972.2A CN201980102972A CN114787193A CN 114787193 A CN114787193 A CN 114787193A CN 201980102972 A CN201980102972 A CN 201980102972A CN 114787193 A CN114787193 A CN 114787193A
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monoclonal antibody
seq
dna polymerase
polymerase
nucleotide sequence
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CN114787193B (zh
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李慧真
高重亮
郑越
郭苗苗
席凤
董宇亮
徐崇钧
章文蔚
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BGI Shenzhen Co Ltd
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BGI Shenzhen Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Abstract

提供了一种抗高保真聚合酶的单克隆抗体及其应用。所提供的单克隆抗体包括重链高变区和轻链高变区，所述重链高变区和所述轻链高变区包括下列至少之一：(1)所述重链高变区包括选自ASGYPFSTY、DKSSST和EGITTLVAPMDY中的至少一种，所述轻链高变区包括选自SSVTYMHWY、RVEAED和KLELKRADAAPT中的至少一种；(2)与(1)相比，所述重链高变区至少具有一个保守氨基酸取代，和/或所述轻链高变区至少具有一个保守氨基酸取代。所提供的单克隆抗体用于聚合酶链式反应中，特异性强，准确性高。

Description

PCT国内申请，说明书已公开。

Claims (18)

  1. PCT国内申请，权利要求书已公开。
CN201980102972.2A 2019-12-19 2019-12-19 抗聚合酶的单克隆抗体及其应用 Active CN114787193B (zh)

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Cited By (4)

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CN115806621A (zh) * 2022-11-25 2023-03-17 厦门康基生物科技有限公司 一种Taq DNA聚合酶的单克隆抗体F2B2及其应用
CN116217730A (zh) * 2022-11-25 2023-06-06 厦门康基生物科技有限公司 一种Taq酶的单克隆抗体F7H6及其应用
CN116284413A (zh) * 2022-11-25 2023-06-23 厦门康基生物科技有限公司 一种Taq DNA聚合酶的单克隆抗体F12H12及其应用
CN116589572A (zh) * 2023-06-28 2023-08-15 湖南诺合新生物科技有限公司 一种抗ha标签的单克隆抗体及其应用

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* Cited by examiner, † Cited by third party
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CN115594768B (zh) * 2022-12-12 2023-03-17 珠海宝锐生物科技有限公司 一种分泌抗dna聚合酶单克隆抗体的杂交瘤细胞、单克隆抗体及其应用

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US5985619A (en) * 1995-02-07 1999-11-16 Clinical Diagnostic Systems, Inc. Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
CN104245956A (zh) * 2012-03-09 2014-12-24 株式会社百奥尼 用于热启动逆转录反应或热启动逆转录聚合酶链式反应的组合物
WO2015085230A1 (en) * 2013-12-06 2015-06-11 Bio-Rad Laboratories, Inc. Fusion polymerases
CN110256566A (zh) * 2019-05-10 2019-09-20 江苏苏博生物医学科技南京有限公司 Taq DNA聚合酶单链免疫球蛋白IgG抗体、制备方法及其在基因分型检测中的应用

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US6274353B1 (en) * 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
JP7116942B2 (ja) * 2017-08-25 2022-08-12 東洋紡株式会社 微生物で抗体を生産する方法

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
US5985619A (en) * 1995-02-07 1999-11-16 Clinical Diagnostic Systems, Inc. Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
CN104245956A (zh) * 2012-03-09 2014-12-24 株式会社百奥尼 用于热启动逆转录反应或热启动逆转录聚合酶链式反应的组合物
WO2015085230A1 (en) * 2013-12-06 2015-06-11 Bio-Rad Laboratories, Inc. Fusion polymerases
CN110256566A (zh) * 2019-05-10 2019-09-20 江苏苏博生物医学科技南京有限公司 Taq DNA聚合酶单链免疫球蛋白IgG抗体、制备方法及其在基因分型检测中的应用

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115806621A (zh) * 2022-11-25 2023-03-17 厦门康基生物科技有限公司 一种Taq DNA聚合酶的单克隆抗体F2B2及其应用
CN116217730A (zh) * 2022-11-25 2023-06-06 厦门康基生物科技有限公司 一种Taq酶的单克隆抗体F7H6及其应用
CN116284413A (zh) * 2022-11-25 2023-06-23 厦门康基生物科技有限公司 一种Taq DNA聚合酶的单克隆抗体F12H12及其应用
CN115806621B (zh) * 2022-11-25 2023-06-30 厦门康基生物科技有限公司 一种Taq DNA聚合酶的单克隆抗体F2B2及其应用
CN116284413B (zh) * 2022-11-25 2023-09-05 厦门康基生物科技有限公司 一种Taq DNA聚合酶的单克隆抗体F12H12及其应用
CN116217730B (zh) * 2022-11-25 2023-09-05 厦门康基生物科技有限公司 一种Taq酶的单克隆抗体F7H6及其应用
CN116589572A (zh) * 2023-06-28 2023-08-15 湖南诺合新生物科技有限公司 一种抗ha标签的单克隆抗体及其应用
CN116589572B (zh) * 2023-06-28 2023-11-10 湖南诺合新生物科技有限公司 一种抗ha标签的单克隆抗体及其应用

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