CN114774266B - Multi-channel integrated sample adding device and method thereof - Google Patents
Multi-channel integrated sample adding device and method thereof Download PDFInfo
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- CN114774266B CN114774266B CN202210203580.9A CN202210203580A CN114774266B CN 114774266 B CN114774266 B CN 114774266B CN 202210203580 A CN202210203580 A CN 202210203580A CN 114774266 B CN114774266 B CN 114774266B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E30/00—Energy generation of nuclear origin
- Y02E30/30—Nuclear fission reactors
Abstract
The application discloses a multichannel integrated sample adding device and a multichannel integrated sample adding method, wherein the multichannel integrated sample adding device comprises a base, a Z-direction driving device, a Z-direction guide rail, a Z-direction sliding block and a sample adding mechanism, the sample adding mechanism can move up and down along the Z-direction guide rail under the driving of the Z-direction driving device, the sample adding mechanism comprises a first motor, a piston assembly and a gun discharging module, the piston assembly is connected to the output end of the first motor, the gun discharging module is arranged at the lower end of the piston assembly, and the piston is communicated with an adapter.
Description
Technical Field
The application relates to the technical field of medical equipment, in particular to a multichannel integrated sample adding device and a multichannel integrated sample adding method.
Background
The full-automatic nucleic acid extractor is based on magnetic bead separation principle methodology, integrates the functions of nucleic acid extraction, sample distribution, reagent distribution, pipetting, magnetic attraction, vibration mixing and the like, generally uses a 96-hole deep pore plate as a test carrier, and can realize the automatic extraction and purification of high-flux and high-purity nucleic acid. The full-automatic nucleic acid extractor generally comprises a sample adding arm module, an oscillation module, a sample test tube rack module, a magnetic rack module and the like.
The full-automatic nucleic acid extractor generally adopts a disposable sample adding needle to absorb liquid and distribute the absorbed liquid into a reaction plate so as to avoid cross contamination, and therefore, the sample adding arm module needs to have the functions of automatic needle taking, liquid sucking, liquid injecting and automatic needle removing. In the prior art, the sample adding channels are fewer, the processing efficiency is low, and the clinical requirements of large-batch nucleic acid extraction and detection cannot be met. On the other hand, in the prior art, the sampling module is often used for controlling the needle taking and needle removing of the channels by arranging a driving module on each channel, and when the channels are more, the sampling arm modules of the channels integrated in the mode have larger volume, so that the whole occupied space of the full-automatic nucleic acid extractor is larger, and the miniaturization of the equipment is not facilitated.
Therefore, research and improvement on the multichannel integrated sample adding device and the method thereof are needed to solve the problems in the prior art.
Disclosure of Invention
The application provides a multichannel integrated sample adding device and a multichannel integrated sample adding method, which aim to overcome the defects in the prior art. The multichannel integrated sample adding device can realize multichannel needle taking, liquid sucking, liquid injecting and needle removing operations, and has the advantages that the operation process is stable, the working efficiency of nucleic acid extraction is improved, the occupied space of the device is small, and the miniaturization of equipment is facilitated.
The application provides a multichannel integrated sample adding device, which comprises:
the utility model provides a multichannel integration application of sample device which characterized in that, includes the base, be provided with Z to drive arrangement, Z to guide rail, set up Z to slider, the sampling mechanism on Z to the guide rail on the base.
Further, the Z-direction driving device can drive the Z-direction sliding block on the Z-direction guide rail to enable the Z-direction sliding block to move up and down on the Z-direction guide rail, the sampling mechanism is arranged on the Z-direction sliding block on the Z-direction guide rail, and the sampling mechanism can move up and down along the Z-direction guide rail under the driving of the Z-direction driving device.
Further, the sampling mechanism comprises a first motor, a piston assembly and a gun discharging module, wherein the piston assembly comprises pistons with the same number as the channels, the gun discharging module comprises adapters with the same number as the channels, the piston assembly is connected to the output end of the first motor, the gun discharging module is arranged at the lower end of the piston assembly, and the pistons are communicated with the adapters.
Further, the number of channels is eight channels.
Further, the piston assembly comprises a piston connecting plate, the pistons are arranged in front and back rows, the first row of pistons and the second row of pistons are arranged at staggered intervals, and the upper ends of the pistons are connected with the piston connecting plate.
Further, the gun discharging module further comprises a gun discharging cavity, a piston mounting position is arranged on the upper portion of the gun discharging cavity, an adapter mounting position is arranged on the lower portion of the gun discharging cavity, the piston mounting positions are arranged in a front-back staggered mode, the adapter mounting positions are arranged in the middle of the two rows of piston mounting positions, the lower end of the piston is inserted into the piston mounting positions, the upper end of the adapter is inserted into the adapter mounting positions, a vent hole is formed in the gun discharging cavity, and the upper end of the adapter and the lower end of the piston are connected through the vent hole to achieve a passage of a gas circuit.
Further, the gun discharging module further comprises a needle discharging plate, a needle discharging rod and a photoelectric switch detecting plate, wherein the needle discharging plate is arranged below the gun discharging cavity, photoelectric switch baffle notch is formed in the edges of the front side and the rear side of the needle discharging plate, a plurality of photoelectric switches are arranged on the photoelectric switch detecting plate, the photoelectric switch detecting plate is arranged above the needle discharging plate, the photoelectric switches correspond to the photoelectric switch baffle notch, and two ends of the needle discharging rod are respectively connected with the gun discharging cavity and the needle discharging plate.
Further, the gun module further comprises a needle removing sleeve with the same number as the channels, the needle removing sleeve is sleeved on the adapter and is arranged on the front row and the rear row of the adapter in a staggered mode, the needle removing sleeve is fixed on the needle removing plate through an anti-removing bolt, a needle removing spring is sleeved on the anti-removing bolt, a photoelectric switch baffle is arranged on the upper portion of the needle removing sleeve, and the photoelectric switch baffle can be inserted into a photoelectric switch baffle notch on the needle removing plate and matched with a photoelectric switch arranged on a photoelectric switch detection plate for needle taking and needle removing in-place detection.
Further, the gun discharging module further comprises an air pressure detection module, wherein the air pressure detection module is arranged on the left side and the right side of the gun discharging cavity and is communicated with a channel inside the piston, and the air pressure detection module is used for detecting the change of liquid capacity during liquid suction or liquid injection.
Further, the air pressure detection module comprises an air pressure detection plate, a sealing ring, a spring and a spring cover, wherein the sealing ring is arranged between the air pressure detection plate and a channel at the lower end of the piston, and the spring cover are sequentially arranged on the outer side of the air pressure detection plate and used for sealing the air pressure detection plate and the channel inside the piston.
The application also provides a sample adding method of the multichannel integrated sample adding device, which comprises the following steps:
the sample adding method of the multichannel integrated sample adding device is characterized by comprising the following steps of:
when the sampling mechanism moves to the position above the needle taking position, the Z-direction driving device drives the sampling mechanism to move downwards, and the tail end of the adapter is inserted into an opening of the sample adding needle to perform needle taking action;
the sampling mechanism moves to the position above the liquid suction level, and when the Z-direction driving drives the sampling needle on the adapter to move downwards to the position below the liquid surface to be sucked, the first motor drives the piston to move upwards, so that the adapter communicated with the piston performs liquid suction work;
the first motor drives the piston to move downwards, so that the adapter communicated with the piston performs liquid injection;
the sampling mechanism moves to the needle removing area, the first motor drives the piston connecting plate to move downwards, the piston connecting plate extrudes the needle removing rod to move downwards, the needle removing rod drives the needle removing plate to move downwards, and the needle removing sleeve is driven to extrude the sample adding needle downwards to achieve needle removing.
Further, the sample adding method further comprises the following steps:
the sample adding needle is propped against the needle removing sleeve to move upwards, and a photoelectric switch baffle sheet on the needle removing sleeve enters a photoelectric switch detection range to perform needle taking in-place detection;
the needle removing plate drives the needle removing sleeve to move downwards, and a photoelectric switch baffle plate on the needle removing sleeve moves out of a detection range of the photoelectric switch to perform needle removing in-place detection;
the first motor drives the piston to move upwards, so that when the adapter communicated with the piston performs liquid suction work, the air pressure detection modules at the left side and the right side of the gun cavity perform air pressure detection;
when the air pressure detection modules at the left side and the right side of the gun discharging cavity receive the synchronous increase of the pressure detection signals, a liquid taking detection normal signal is sent out;
when the air pressure detection modules at the left side and the right side of the gun discharging cavity receive the synchronous reduction of the pressure detection signals, a normal signal for liquid injection detection is sent out;
when the air pressure detection modules at the left side and the right side of the gun cavity receive the asynchronous pressure detection signals, abnormal signals of liquid taking or liquid injection are sent out.
Compared with the prior art, the multichannel integrated sample adding device comprises a base, a Z-direction driving device, a Z-direction guide rail, a Z-direction sliding block and a sample adding mechanism, wherein the sample adding mechanism can move up and down along the Z-direction guide rail under the driving of the Z-direction driving device, the sample adding mechanism comprises a first motor, a piston assembly and a gun discharging module, the piston assembly is connected to the output end of the first motor, the gun discharging module is arranged at the lower end of the piston assembly, the piston is communicated with an adapter, and the multichannel integrated sample adding device realizes the operations of taking, absorbing liquid, injecting liquid, taking off the needle and the like of a multichannel through the integral matching of the driving device, the piston assembly and the gun discharging module, solves the problems of fewer parallel channels and low efficiency in the prior art, and can improve the continuity of needle taking, absorbing liquid, injecting liquid and needle releasing operation and greatly improves the working efficiency compared with the multichannel integrated sample adding device in the prior art.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments described in the present application, and other drawings may be obtained according to these drawings for a person having ordinary skill in the art.
FIG. 1 is a schematic diagram of a multi-channel integrated sample application device according to an embodiment of the present application;
FIG. 2 is a schematic exploded view of a multi-channel integrated sample application device according to an embodiment of the present application;
FIG. 3 is an exploded view of a sample application mechanism according to an embodiment of the present application;
FIG. 4 is a top view of a lance cavity in accordance with one embodiment of the application;
FIG. 5 is a longitudinal section of a gun cavity according to an embodiment of the present application;
FIG. 6 is a schematic diagram of a sample loading mechanism according to an embodiment of the present application;
FIG. 7 is a schematic view of a first view angle of the sample loading mechanism according to an embodiment of the present application;
FIG. 8 is a schematic diagram of a second view angle structure of the sample loading mechanism according to an embodiment of the present application;
FIG. 9 is a schematic view illustrating a third view angle of the sample loading mechanism according to an embodiment of the present application;
fig. 10 is a top view of a stripper plate according to an embodiment of the present application;
FIG. 11 is a schematic view of a cavity structure of a gun according to an embodiment of the present application;
FIG. 12 is a longitudinal section of a gun cavity according to an embodiment of the present application.
The device comprises a 100-multichannel integrated sample adding device, a 110-base, a 111-Z-direction driving device, a 112-Z-direction guide rail, a 113-Z-direction sliding block, a 120-sample adding mechanism, a 121-first motor, a 122-motor fixing plate, a 123-guide rod, a 124-oil seal fixing plate, a 125-oil seal, a 130-gun arranging module, a 131-adapter, a 132-gun arranging cavity, a 133-piston mounting position, a 134-adapter mounting position, a 135-vent hole, a 136-needle removing plate, a 137-needle removing rod, a 140-piston assembly, a 141-piston, a 142-piston connecting plate, a 143-liquid suction photoelectric switch baffle, a 150-photoelectric switch detecting plate, a 151-photoelectric switch baffle notch, a 152-needle removing photoelectric switch, a 153-needle removing sleeve, a 154-anti-needle removing bolt, a 155-needle removing spring, a 156-needle removing photoelectric switch baffle, a 160-air pressure detecting module, a 161-air pressure detecting plate, a 162-sealing ring, a 163-spring, a 164-spring cover, a 170-drag chain, a 171-gun cover, a 172-gun arranging cover, a 172-Z-gun arranging cover, a 173-liquid suction photoelectric switch baffle, a 173-Z-liquid suction switch fixing plate, a 178-photoelectric switch, a Z-direction photoelectric switch fixing plate, a Z-direction baffle, and a fan-plate.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present application more apparent, embodiments of the present application will be further described with reference to the accompanying drawings. It will be apparent that the described embodiments are only some, but not all, embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
It should be noted that all directional indicators (such as up, down, left, right, front, rear, etc.) in the embodiments of the present application are merely used to explain the relative positional relationship between the components, the movement situation, etc. in a specific posture, and if the specific posture is changed, the directional indicators are correspondingly changed.
As shown in fig. 1-2, a multi-channel integrated sample loading device 100 includes a base 110, a Z-direction driving device 111, a Z-direction rail 112, Z-direction sliders 113 disposed on the Z-direction rail 112, and a sample loading mechanism 120 disposed on the base 110, wherein the Z-direction driving device 111 can drive the Z-direction sliders 113 on the Z-direction rail 112 to move up and down on the Z-direction rail 112, the sample loading mechanism 120 is disposed on the Z-direction sliders 113 on the Z-direction rail 112, the sample loading mechanism 120 can move up and down along the Z-direction rail 112 under the driving of the Z-direction driving device 111, the sample loading mechanism 120 includes a first motor 121, a piston assembly 140, and a gun discharging module 130, the piston assembly 140 includes the same number of pistons 141 as the channels, the gun discharging module 130 includes the same number of adapters 131, the piston assembly 140 is connected to the output end of the first motor 121, the gun discharging module 130 is disposed at the lower end of the piston assembly 140, and the piston 141 is connected to the adapter 131 in a penetrating manner.
This multichannel integration application of sample device 100 is through the whole cooperation of structures such as Z to drive arrangement 111, first motor 121, piston assembly 140 and row rifle module 130, realizes operations such as the needle of getting of multichannel, imbibition, annotate liquid, take off the needle, compares prior art, and this multichannel integration application of sample device 100 can improve the continuity of getting the needle, imbibition, annotate liquid, take off the needle operation, very big improvement work efficiency, the problem of parallel channel less among the solution prior art, inefficiency to multichannel application of sample equipment size is great among the prior art, the integrated level is low, the problem of flexibility ratio is low.
As shown in fig. 1-2, the number of channels of the multi-channel integrated loading device 100 is eight channels. In general, a nucleic acid extractor is used in combination with a 96-well deep-hole plate for extracting nucleic acids, and the 96-well deep-hole plate is generally 8 rows and 12 columns. The 8 channels of the multi-channel integrated sample adding device 100 correspond to 8 holes in each row of the 96-hole deep hole plate, so that sample adding operation of 8 holes in one row can be finished at a time, the working efficiency is greatly improved, and meanwhile, the problem of poor precision caused by multiple sample adding in each row is avoided. Of course, the multi-channel integrated sample loading device 100 can be set to 2-7 channels according to the actual experiment requirement, and the general concept of the application is not affected.
As shown in fig. 2-3 and fig. 9, the piston assembly 140 includes a piston connecting plate 142, the pistons 141 are arranged in front and rear rows, the pistons of the first row and the pistons of the second row are arranged at staggered intervals, and the upper end of the piston 141 is connected with the piston connecting plate 142. The pistons 141 of the multi-channel integrated sample application device 100 are tightly connected to the gun cavity 132 via piston connecting plates 142. The multichannel integrated sample adding device 100 can change the air pressure in the piston through the up-and-down movement of the piston, so as to realize the operations of liquid suction and liquid injection, and the piston needs to meet certain compression volume change in order to meet the requirement of liquid suction and liquid injection of certain volume of each channel in the experimental process. According to the multi-channel integrated sample adding device 100, the two rows of pistons are arranged, the first row of pistons and the second row of pistons are arranged at intervals in a staggered manner, so that the pistons can be arranged to have a wider outer diameter, and the length of the pistons can have a shorter distance under the condition of meeting the same compression volume of the pistons, so that the multi-channel integrated sample adding device 100 is more exquisite in structure, higher in space utilization rate and beneficial to miniaturization of equipment.
As shown in fig. 2-5, the gun discharging module 130 further includes a gun discharging cavity 132, a piston mounting position 133 is provided at an upper portion of the gun discharging cavity 132, an adapter mounting position 134 is provided at a lower portion of the gun discharging cavity 132, the piston mounting position 133 is provided for two front and rear staggered positions, the adapter mounting position 134 is provided in a middle of the two rows of the piston mounting positions 133, a lower end of the piston 141 is inserted in the piston mounting position 133, an upper end of the adapter 131 is inserted in the adapter mounting position 134, a vent hole 135 is provided in the gun discharging cavity 132, and a passage of an air path is realized between an upper end of the adapter 131 and a lower end of the piston 141 through the vent hole 135. The multichannel integrated sample adding device 100 is connected with a multichannel piston 141 and an adapter 131 through a gun cavity 132, a piston mounting position 133 on the upper part of the gun cavity 132 is matched with the piston, and is also arranged in a front-back staggered mode, the piston 141 is tightly connected with the gun cavity 132 through an oil seal 125, the adapter 131 is tightly connected with an adapter mounting position 134 on the lower part of the gun cavity 132, and the adapter mounting position 134 is arranged in a single row and corresponds to the multichannel adapter. As shown in fig. 5, a vent hole 135 is provided in the gun cavity 132, and when the piston 141 and the adapter 131 are both inserted into the gun cavity 132, the upper end air outlet hole of the adapter 131 and the lower end air outlet hole of the piston 141 realize the passage of the air path through the vent hole 135, thereby realizing the communication of the air path during the operations of sucking and injecting the liquid of the whole device. Due to the staggered arrangement of the adapter and the piston, the multichannel integrated sample adding device 100 is more exquisite in structure and higher in space utilization rate, and is beneficial to miniaturization of equipment.
As shown in fig. 2, 6 and 8, the gun module 130 further includes a needle release plate 136, a needle release rod 137 and a photoelectric switch detection plate 150, the needle release plate 136 is disposed below the gun cavity 132, the photoelectric switch blocking slots 151 are formed at the edges of the front and rear sides of the needle release plate 136, a plurality of needle release photoelectric switches 152 are disposed on the photoelectric switch detection plate 150, the photoelectric switch detection plate 150 is disposed on the front and rear sides of the needle release plate 136, and the needle release photoelectric switches 152 correspond to the photoelectric switch blocking slots 151, and two ends of the needle release rod 137 are respectively connected with the piston connecting plate 142 and the needle release plate 136. Further, a spring is provided at the upper end of the needle bar 137 to be connected with the piston connecting plate 142. When the needle removing operation is required, the first motor 121 drives the piston connecting plate 142 to move downwards, the piston connecting plate 142 compresses the needle removing rod 137, the needle removing rod 137 pushes the needle removing plate 136 to move downwards, and the needle removing plate 136 pushes the disposable sample adding needle on the adapter to move downwards, so that the needle removing operation is completed. The front and rear side edges of the needle removing plate 136 are provided with photoelectric switch baffle notch 151 for inserting the needle removing photoelectric switch baffle 156 on the needle removing sleeve 153, the front and rear of the needle removing plate 136 are also provided with photoelectric switch detecting plates 150, each photoelectric switch detecting plate 150 is provided with a plurality of needle removing photoelectric switches 152 side by side, namely, each channel is correspondingly provided with a needle removing photoelectric switch 152, and the needle removing and needle removing in-place detection of each channel can be accurately realized.
As shown in fig. 2, the gun module 130 further includes a Z-direction photoelectric switch 179 for detecting the Z-direction movement position of the gun module 130. The gun discharging module 130 is provided with a Z-direction photoelectric switch baffle 178 corresponding to the Z-direction photoelectric switch 179, and when the gun discharging module 130 moves in place, the Z-direction photoelectric switch baffle 178 enters the sensing range of the Z-direction photoelectric switch 179, so that Z-direction movement in-place detection of the gun discharging module 130 is realized.
As shown in fig. 2 and fig. 7-9, the gun module 130 further includes a needle removing sleeve 153 with the same number as the channels, the needle removing sleeve 153 is sleeved on the adapter 131 and is alternately arranged on the front row and the rear row of the adapter 131, the needle removing sleeve 153 is fixed on the needle removing plate 136 through a release preventing bolt 154, a needle removing spring 155 is sleeved on the release preventing bolt 154, a needle taking photoelectric switch baffle 156 is arranged on the upper portion of the needle removing sleeve 153, and the needle taking photoelectric switch baffle 156 can be inserted into a photoelectric switch baffle notch on the needle removing plate to be matched with a photoelectric switch arranged on the photoelectric switch detection plate for detecting needle taking and needle removing in place. As shown in fig. 10, the needle removing sleeves 153 are staggered in the front and rear rows of the adapter 131, so that depth space is fully utilized in a limited length range of the multi-channel adapter arranged side by side, smooth needle removing and needle removing operations are ensured, in-place detection can be accurately performed, the structure of the gun discharging module 130 is more exquisite, the space utilization rate is higher, and the miniaturization of equipment is facilitated. When the needle taking photoelectric switch baffle 156 on the needle taking sleeve 153 enters the detection range of the needle taking photoelectric switch 152, namely, the disposable sample injection needle is taken in place, the needle taking photoelectric switch 152 receives the induction signal; when the needle taking photoelectric switch blocking piece 156 on the needle taking sleeve 153 leaves the detection range of the needle taking photoelectric switch 152, namely, the needle taking plate 136 pushes the needle taking sleeve 153 to perform the needle taking operation in place, the needle taking photoelectric switch 152 has no induction signal, and thus the needle taking in place and the needle taking in place detection are realized.
As shown in fig. 2, 11 and 12, the gun discharging module 130 further includes an air pressure detecting module 160, where the air pressure detecting module 160 is disposed at the left and right sides of the gun discharging cavity 132 and communicates with the channel inside the piston for detecting the change of the liquid capacity during the liquid sucking or filling. The air pressure detection module 160 is communicated with the internal channel of the piston through the vent hole in the gun cavity 132, and can detect the air pressure change in the channel when the piston moves up and down in real time, thereby detecting the change of the liquid capacity in the channel and further detecting the liquid suction or injection action. The detection modules 160 are respectively arranged at the left side and the right side of the gun discharging cavity 132, so that the condition that the air pressure detection is inaccurate due to the blockage of the middle respective channels can be prevented, if the detection modules 160 at the left side and the right side of the gun discharging cavity 132 have the same change trend, the liquid suction or liquid injection function is reflected to be normal, and if the change trend of the detection modules 160 at the left side and the right side of the gun discharging cavity 132 is inconsistent, the blockage of a certain channel or the abnormal liquid suction and liquid injection are reflected. Further, when the air pressure detection modules 160 at the left and right sides of the gun discharging cavity 132 receive the synchronous increase of the pressure detection signal, a liquid taking detection normal signal is sent; when the air pressure detection modules 160 at the left side and the right side of the gun discharging cavity 132 receive the synchronous reduction of the pressure detection signals, a normal signal for liquid injection detection is sent out; when the air pressure detection modules 160 at the left and right sides of the gun discharging cavity 132 receive the pressure detection signals and are asynchronous, abnormal signals of liquid taking or liquid injection are sent out.
As shown in fig. 2, 11 and 12, the air pressure detection module 160 includes an air pressure detection plate 161, a sealing ring 162, a spring 163 and a spring cover 164, the sealing ring 162 is disposed between the air pressure detection plate 161 and a channel at the lower end of the piston, and the spring 163 and the spring cover 164 are sequentially disposed on the outer side of the air pressure detection plate 161, so as to seal the air pressure detection plate 161 and the channel inside the piston 141. The pressure detection plate 161 realizes the sealing between the pressure detection plate 161 and the internal channel through the tension of the inner sealing ring 162 and the spring cover 164 to the external spring 163, ensures the accuracy of the pressure detection, has simple and exquisite structure and high space utilization rate, and is beneficial to the miniaturization of equipment.
The multi-channel integrated sample loading device 100 further comprises a control device, wherein the control device is electrically connected with components such as the Z-direction driving device 111, the first motor 121, the needle-removing photoelectric switch 152, the air pressure detection plate 161, the Z-direction photoelectric switch 179 and the like respectively, and is used for sending out movement instructions of all modules, receiving detection signals of the photoelectric switch and the air pressure detection module, comparing the detection signals and sending out abnormal information prompts.
The application also provides a sample adding method of the multichannel integrated sample adding device, which adopts the multichannel integrated sample adding device, and comprises the following steps:
when the sampling mechanism 120 moves to above the needle taking position, the Z-direction driving device 111 drives the sampling mechanism 120 to move downward, and the end of the adapter 131 is inserted into the opening of the sample taking needle to take the needle. The sampling mechanism 120 can move according to the placement position of the sampling needle, has X, Y and Z-direction freedom degree, high flexibility and accurate positioning, and the Z-direction driving device drives the sampling mechanism 120 to move downwards through the cooperation of the Z-direction guide rail and the Z-direction sliding block, so that the sampling mechanism 120 has higher power, the friction resistance between the adapter and the opening of the sampling needle during needle taking is overcome, the occurrence probability of needle taking abnormality is lower, and the needle taking action is more stable.
When the sampling mechanism 120 moves to the position above the liquid sucking position and the sampling needle on the adapter 131 is driven by the Z-direction driving device 111 to move downwards to the position below the liquid surface to be sucked, the piston 141 is driven by the first motor 121 to move upwards, so that the adapter 131 communicated with the piston 141 performs liquid sucking operation.
The sampling mechanism 120 moves to the sampling position, and the first motor 121 drives the piston 141 to move downwards, so that the adapter communicated with the piston 141 performs the liquid injection work. The first motor 121 is an advanced motor, and the operation of sucking and injecting liquid is realized through the forward rotation or the reverse rotation of the first motor 121, and the sucking and injecting liquid process is more accurate and stable.
The sampling mechanism 120 moves to the needle removing area, the first motor 121 drives the piston connecting plate 142 to move downwards, the piston connecting plate 142 extrudes the needle removing rod 137 to move downwards, the needle removing rod 137 drives the needle removing plate 136 to move downwards, and the needle removing sleeve 153 is further driven to extrude the sample adding needle downwards to achieve needle removing. After the first motor 121 drives the piston 141 to move downwards to perform injection, the first motor 121 continues to drive the piston connecting plate 142 to move downwards, so that the needle removing rod 137 is extruded, the needle removing plate 136 is pushed to move downwards, and the needle removing action is completed. The first motor 121 can not only drive and realize the liquid sucking and injecting operation, but also drive and realize the needle removing operation, so that the integration level of the multichannel integrated sample adding device is improved, the structure is simple and exquisite, the space utilization rate is high, and the miniaturization of equipment is facilitated.
Further, the sample adding method of the multichannel integrated sample adding device is characterized by further comprising the following steps:
the sample injection needle pushes the needle removing sleeve 153 to move upwards, and the needle taking photoelectric switch blocking piece 156 on the needle removing sleeve 153 enters the detection range of the needle removing photoelectric switch 152 to perform needle taking in-place detection. Each channel adapter corresponds to one needle taking photoelectric switch baffle 156 and one needle taking-off photoelectric switch 152, so that each channel can realize in-place needle taking detection, and the detection accuracy is high. After the needle taking action, if a certain channel does not detect the induction signal of the needle-removing photoelectric switch 152, the channel is prompted to fail in needle taking, and the needle taking condition of each channel can be accurately detected.
The needle removing plate 136 drives the needle removing sleeve 153 to move downwards, and the needle removing photoelectric switch blocking piece 156 on the needle removing sleeve 153 moves out of the detection range of the needle removing photoelectric switch 152 to perform needle removing in-place detection. The needle taking photoelectric switch baffle 156 and the needle taking photoelectric switch 152 are matched to realize needle taking in-place detection, which is beneficial to high integration and miniaturization of equipment. Each channel can realize the in-place detection of needle removal, and the detection accuracy is high. After the needle removing action, if a certain channel still detects the sensing signal of the needle removing photoelectric switch 152, the channel is prompted to fail in needle removing, and the needle removing condition of each channel can be accurately detected.
When the first motor 121 drives the piston 141 to move upwards and the adapter 131 communicated with the piston 141 performs liquid suction operation, the air pressure detection modules 160 on the left side and the right side of the gun discharging cavity 132 perform air pressure detection. When the air pressure detection modules at the left side and the right side of the gun discharging cavity receive the synchronous increase of the pressure detection signals, a liquid taking detection normal signal is sent out; when the air pressure detection modules at the left side and the right side of the gun discharging cavity receive the synchronous reduction of the pressure detection signals, a normal signal for liquid injection detection is sent out; when the air pressure detection modules at the left side and the right side of the gun cavity receive the asynchronous pressure detection signals, abnormal signals of liquid taking or liquid injection are sent out. The detection modules 160 are respectively arranged at the left side and the right side of the gun discharging cavity 132, so that the situation that the air pressure detection is inaccurate due to the blockage of the middle respective channels can be prevented, if the detection modules 160 at the left side and the right side of the gun discharging cavity 132 have the same change trend, the liquid suction or liquid injection function is reflected to be normal, and if the change trend of the detection modules 160 at the left side and the right side of the gun discharging cavity 132 is inconsistent, the blockage of a certain channel or the abnormal liquid suction and liquid injection of a certain channel is reflected, and the real-time detection of the liquid suction and liquid injection conditions of each channel is realized.
According to the multichannel integrated sample adding device provided by the application, the operation of taking the needle, sucking the liquid, injecting the liquid, taking off the needle and the like of the multichannel is realized through the integral matching of the driving device, the piston assembly and the gun discharging module, the problems of fewer parallel channels and low efficiency in the prior art are solved, the problems of larger size, low integration level and low flexibility of multichannel sample adding equipment in the prior art are solved, and compared with the prior art, the multichannel integrated sample adding device can perform the operation of taking the needle, sucking the liquid, injecting the liquid and taking off the needle of the multichannel, and the detection of taking the needle, sucking the liquid, injecting the liquid and taking off the needle of the multichannel can be realized, so that the working efficiency is greatly improved.
Applicant states that the above-described embodiments merely convey the general principles, features, and advantages of the application. It will be understood by those skilled in the art that the present application is not limited to the embodiments described above, and that the embodiments and descriptions described herein are merely illustrative of the principles of the application, and that various changes and modifications may be made by those skilled in the art without departing from the spirit and scope of the application, which is defined by the appended claims.
The present application is not limited to the above embodiments, and all embodiments using structures similar to the present application and methods for achieving the objects of the present application are within the scope of the present application.
Claims (8)
1. The multichannel integrated sample adding device is characterized by comprising a base, wherein a Z-direction driving device, a Z-direction guide rail, a Z-direction sliding block arranged on the Z-direction guide rail and a sample adding mechanism are arranged on the base;
the Z-direction driving device can drive the Z-direction sliding block on the Z-direction guide rail to enable the Z-direction sliding block to move up and down on the Z-direction guide rail, the sampling mechanism is arranged on the Z-direction sliding block on the Z-direction guide rail, and the sampling mechanism can move up and down along the Z-direction guide rail under the driving of the Z-direction driving device;
the utility model provides a heating mechanism, including first motor, piston subassembly, row rifle module, the piston subassembly include with the piston of passageway quantity the same, the piston subassembly still includes the piston connecting plate, the piston is two rows of settings around, and first row piston and second row piston dislocation interval set up, the upper end of piston with the piston connecting plate is connected, the row rifle module includes the adapter the same with passageway quantity, the piston subassembly connect in the output of first motor, first motor can drive the piston upward or downward movement, the row rifle module set up in the lower extreme of piston subassembly, the piston with the adapter link up and link up, the row rifle module still includes row rifle cavity, the upper portion of row rifle cavity is equipped with the piston mount position, the lower part of row rifle cavity is equipped with the adapter mount position, the lower extreme of piston inserts and locates the piston mount position, the upper end of adapter inserts and locates the adapter mount position, the piston mount position is two rows of position settings around, the adapter mount position is in the piston mount position is located the middle of two and is equipped with the adapter and is equipped with the air vent through the passageway is located to the adapter, the air circuit is equipped with the corresponding to arrange the air vent.
2. The multi-channel integrated loading device of claim 1 wherein the number of channels is eight channels.
3. The multi-channel integrated loading device of claim 1, wherein: the gun discharging module further comprises a needle discharging plate, needle discharging rods and photoelectric switch detecting plates, the needle discharging plate is arranged below the gun discharging cavity, photoelectric switch baffle notch openings are formed in the edges of the front side and the rear side of the needle discharging plate, a plurality of needle discharging photoelectric switches are arranged on the photoelectric switch detecting plates, the photoelectric switch detecting plates are arranged on the front side and the rear side of the needle discharging plate, the photoelectric switches correspond to the photoelectric switch baffle notch openings, and two ends of the needle discharging rods are respectively connected with the piston connecting plate and the needle discharging plate.
4. The multi-channel integrated sample loading device according to claim 3, wherein the gun module further comprises a needle removing sleeve with the same number as the channels, the needle removing sleeve is sleeved on the adapter and is arranged on the front row and the rear row of the adapter in a staggered mode, the needle removing sleeve is fixed on the needle removing plate through a thread removing bolt, the thread removing bolt is sleeved with a needle removing spring, the upper portion of the needle removing sleeve is provided with a needle removing photoelectric switch baffle, and the needle removing photoelectric switch baffle can be inserted into a photoelectric switch baffle notch on the needle removing plate and is matched with a photoelectric switch arranged on a photoelectric switch detection plate for detecting needle removing and needle removing in place.
5. The multi-channel integrated sample loading device according to claim 4, wherein the gun discharging module further comprises an air pressure detection module, wherein the air pressure detection module is arranged at the left side and the right side of the gun discharging cavity, is communicated with the channel inside the piston, and is used for detecting the change of liquid capacity during liquid suction or liquid injection.
6. The multi-channel integrated sample loading device according to claim 5, wherein the air pressure detection module comprises an air pressure detection plate, a sealing ring, a spring and a spring cover, the sealing ring is arranged between the air pressure detection plate and a channel at the lower end of the piston, and the spring cover are sequentially arranged on the outer side of the air pressure detection plate and used for sealing the air pressure detection plate and the channel inside the piston.
7. A method of loading a multichannel integrated loading device, characterized in that the method uses the multichannel integrated loading device according to claim 5 or 6, the method comprising the steps of:
when the sampling mechanism moves to the position above the needle taking position, the Z-direction driving device drives the sampling mechanism to move downwards, and the tail end of the adapter is inserted into an opening of the sample adding needle to perform needle taking action;
the sampling mechanism moves to the position above the liquid suction level, and when the Z-direction driving device drives the sampling needle on the adapter to move downwards to the position below the liquid surface to be sucked, the first motor drives the piston to move upwards, so that the adapter communicated with the piston performs liquid suction work;
the first motor drives the piston to move downwards, so that the adapter communicated with the piston performs liquid injection;
the sampling mechanism moves to the needle removing area, the first motor drives the piston connecting plate to move downwards, the piston connecting plate extrudes the needle removing rod to move downwards, the needle removing rod drives the needle removing plate to move downwards, and the needle removing sleeve is driven to extrude the sample adding needle downwards to achieve needle removing.
8. The method of loading a multi-channel integrated loading apparatus of claim 7, further comprising the steps of:
the sample adding needle is propped against the needle removing sleeve to move upwards, and a needle taking photoelectric switch baffle plate on the needle removing sleeve enters a detection range of the needle removing photoelectric switch to perform needle taking in-place detection;
the needle removing plate drives the needle removing sleeve to move downwards, and a needle removing photoelectric switch baffle sheet on the needle removing sleeve leaves the detection range of the needle removing photoelectric switch to perform needle removing in-place detection;
the first motor drives the piston to move upwards, so that when the adapter communicated with the piston performs liquid suction work, the air pressure detection modules at the left side and the right side of the gun cavity perform air pressure detection;
when the air pressure detection modules at the left side and the right side of the gun discharging cavity receive the synchronous increase of the pressure detection signals, a liquid taking detection normal signal is sent out;
when the air pressure detection modules at the left side and the right side of the gun discharging cavity receive the synchronous reduction of the pressure detection signals, a normal signal for liquid injection detection is sent out;
when the air pressure detection modules at the left side and the right side of the gun cavity receive the asynchronous pressure detection signals, abnormal signals of liquid taking or liquid injection are sent out.
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