CN114748608A - Bead chewing gum pill for resisting oral cavity helicobacter pylori explosion and preparation method thereof - Google Patents

Abstract

The invention discloses a bead chewing gum pill for resisting oral helicobacter pylori explosion, which is prepared from (by weight parts) Glycyrrhrizae radix 0.1-2.5; 0.01-1.0% of tea polyphenol; 1.0-20.0% of probiotics; 0.1-5.0 parts of cow milk protein; 0.5-8.0% of menthol; 0.1-0.5% of a sweetening agent; 6-8 parts of gelatin; 0.5-2% of glycerol; edible vegetable oil, adding to 100 weight parts. The chewing gum pill of the invention applies the principle of cutting off the oral transmission path of helicobacter pylori, has the advantages of quickly eliminating halitosis, inhibiting and killing oral HP and pathogenic bacteria, maintaining normal micro-ecology of oral cavity and the like, and can be used by various people. In addition, the invention also discloses a preparation method of the chewing gum pills.

Description

Bead chewing gum pill for resisting oral cavity helicobacter pylori explosion and preparation method thereof

Technical Field

The invention relates to a chewing gum, in particular to a bead chewing gum pill for resisting helicobacter pylori explosion in oral cavity; in addition, the invention also relates to a preparation method of the bead chewing gum pill for resisting the helicobacter pylori explosion of the oral cavity.

Background

Helicobacter Pylori (HP) is defined as an infectious disease that is transmitted orally, and HP in the stomach has a close relationship with HP in the oral cavity. 70-80% of gastric ulcer and 95% of duodenal ulcer are related to the gastric ulcer, and the risk of gastric cancer can be increased by 4-6 times. As a first carcinogenic source of human gastric cancer, the human gastric cancer is listed by the world health organization as early as 1994 and is an important pathogenic factor of diseases such as chronic gastritis, peptic ulcer, gastric cancer and the like. It is also associated with diseases of other systems outside the gastrointestinal tract, such as iron deficiency anemia, skin diseases, idiopathic thrombocytopenic purpura, coronary heart disease, hypertension, halitosis, oral ulcer, periodontitis, etc. Data from the american microbiology society, journal of clinical microbiology, 2010, show HP infection in about half of the world population. In China, according to epidemiological investigation of the Chinese medical society, the average HP infection rate is higher in China, and is about 59%. This figure is much higher than hepatitis b (8%) and tuberculosis (0.5%. 2010, department of health data). Kyoto viral consensus on Helicobacter pylori university in 2014 suggested that the major period of HP infection was acquired by an intra-family translocation route in childhood (under 12 years, developed countries). Therefore, measures such as keeping good personal life and hygiene habits, washing hands in time, avoiding meals, keeping oral hygiene and the like are measures for reducing the infection rate.

Helicobacter pylori propagates from human to human via the mouth-to-mouth, stomach-to-mouth and/or feces-to-mouth routes. The fecal-oral route mainly occurs in countries/regions with poor hygienic and economic conditions, while oral-oral transmission is probably the most important route in our country. Currently, only the removal of HP in the stomach is considered in the clinical treatment of digestive system diseases, and the infection and the spread control of HP in the oral cavity are ignored. HP radical treatment mostly adopts triple or quadruple therapy, and antibacterial drugs must be used according to the prescriptions, sufficient quantities and specifications. The disadvantages are that: the application of a large amount of antibacterial drugs increases the drug resistance rate and drug resistance of HP.

The chewing gum is particularly popular with young people, has the functions of keeping the faint scent of the oral cavity and exercising facial muscles, but can reflexively secrete a large amount of gastric acid after being chewed for a long time, and the symptoms are aggravated particularly for people with stomach diseases. The existing upgraded bead-popping chewing gum pill can be chewed in a buccal manner and also can be directly swallowed, so that fresh and tasteless breath can be realized, and the defect that the residues are very difficult to clear due to strong viscosity of the traditional chewing gum matrix is avoided.

Disclosure of Invention

According to the embodiment, the invention aims to provide the bead chewing pill for resisting the helicobacter pylori explosion in the oral cavity, which can quickly eliminate halitosis, keep the oral cavity fresh for a long time, kill oral HP and pathogenic bacteria and keep the normal micro-ecology in the oral cavity; in addition, the invention also provides a preparation method of the bead chewing gum pill for resisting the helicobacter pylori explosion of the oral cavity.

According to the embodiment, the invention provides an anti-oral helicobacter pylori bead chewing gum pill which is composed of the following components in parts by weight:

0.1 to 2.5 portions of liquorice;

0.01-1 of tea polyphenol;

1-20 parts of probiotics;

0.1-5 of cow milk protein;

0.5-8 parts of menthol;

0.1-0.5 of sweetening agent;

6-8 parts of gelatin;

0.5-2 parts of glycerol;

edible vegetable oil, adding to 100 weight parts.

According to the embodiment, the preparation method of the bead chewing gum pill for resisting the helicobacter pylori explosion in the oral cavity comprises the following steps:

(1) preparing an external coating wall material: weighing glycerol and gelatin, placing the glycerol and the gelatin into a preparation kettle at 30-45 ℃, uniformly stirring, measuring the effective viscosity of 1000-1550cp, and standing for later use;

(2) preparing a core material: weighing liquorice, tea polyphenol, milk protein, menthol and a sweetening agent, uniformly mixing, and adding probiotics as a core material;

(3) respectively introducing the prepared wall material and core material into a dripping pill machine, dripping the wall material and the core material into edible vegetable oil at 30-45 deg.C, shrinking by concentric multi-layer drippers under the action of surface tension to obtain dripping pills, cooling and cleaning with purified water to obtain wet pills;

(4) drying the wet pills to obtain dry exploded beads, cleaning the dried exploded beads with edible ethanol to remove vegetable oil and damaged exploded beads attached to the surfaces, and air-drying to obtain the oral cavity helicobacter pylori explosion-resistant bead chewing gum pills with the water content of 8-12%.

The invention relates to liquorice and a traditional Chinese medicine name. Is dried root and rhizome of Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, or Glycyrrhiza glabra L. Distributed in northeast, northeast China, Shaanxi, Gansu, Qinghai, Xinjiang, Shandong, etc. Has the effects of invigorating spleen and replenishing qi, clearing away heat and toxic materials, eliminating phlegm and stopping cough, relieving spasm and pain, and harmonizing the medicines. It is indicated for spleen and stomach deficiency, lassitude and hypodynamia, palpitation and shortness of breath, cough with profuse sputum, spasm and pain of abdomen and limbs, carbuncle and sore, and to relieve toxicity and strong action of drugs. In recent years, the discovery has remarkable activities of resisting inflammation, hepatitis B virus, AIDS virus and the like and maintaining the immune balance of organisms, and the main pharmacological components of the composition have the antibacterial effect of glycyrrhizic acid and licoflavone on Hp, which is equivalent to the effect of inhibiting and killing Hp by common antibiotics such as clarithromycin, amoxicillin, furazolidone and the like.

The tea polyphenol is a tea extract, and has the following English name: tea Polyphenols; and the name is as follows: tea tannin, chemical formula C17H19N3O, molecular weight 281.36. Tea polyphenol is the main component forming the color, aroma and taste of tea and is the main component of tea with health care function. Tea polyphenol is the general term of polyphenol substances in tea, and comprises flavanols, anthocyanins, flavonoids, flavonols, phenolic acids and the like, wherein the flavanol catechin is the most important substance. The tea polyphenols can inhibit calcium reduction of human body, effectively prevent dental caries, and has effects of protecting and consolidating teeth. Meanwhile, the components such as vitamin C in the tea can reduce the turbidity of eye crystals and has the effects of reducing eye diseases and protecting eyes and improving eyesight. The tea polyphenols have effects of removing toxic substance and resisting radiation, and can effectively prevent radioactive substance from invading and rapidly discharge out of body. The tea polyphenol in the invention mainly plays roles in maintaining normal ecology of oral cavity, protecting teeth and preventing decayed teeth.

The probiotics comprise one or more of lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium animalis, bifidobacterium lactis and lactobacillus rhamnosus; further preferred is the case. Lactobacillus reuteri is a broad-spectrum effective antibacterial substance, and can inhibit the growth of Escherichia, Salmonella, Shigella, Listeria, Vibrio, Clostridium, and Staphylococcus. Research shows that the concentration of the Roisella is 15-30 mu g/ml, the Roisella can inhibit the growth of gram-positive bacteria, gram-negative bacteria, yeast, fungi and protozoa, is harmless to human and animals, and has wide potential application in the aspects of biological bacteriostat, anti-infection therapeutic agent, biological cross-linking agent, precursor of novel biological materials and the like. Roiximab can be used as chewing gum additive for killing pathogenic bacteria of Hp in oral cavity, preventing dental caries, and relieving halitosis. The lactobacillus has obvious inhibition on oral pathogens and has inhibition on halitosis-related bacteria; the bifidobacterium is used as a physiological beneficial bacterium, and has various important physiological functions of biological barrier, nutrition, anti-tumor, immunity enhancement, gastrointestinal tract function improvement, aging resistance and the like on human health.

The cow milk protein is a general term for a plurality of protein mixtures in cow milk. Mainly comprises two parts of casein (casein) and lactalbumin (lacto-bumin). The former is protein precipitated from milk at 20 deg.C and pH 4.6, and the rest of the protein dissolved in whey is called whey protein. The lactalbumin comprises beta-lactoglobulin, alpha-lactalbumin, serum albumin, immunoglobulin and other trace proteins, such as copper, zinc-superoxide dismutase, etc. All caseins and whey proteins are products of milk secretory cell synthesis in the mammary gland, except for serum albumin, immunoglobulins from blood. The milk protein is rich in cysteine and methionine, can maintain the level of antioxidants in a human body, and is an essential substance indispensable to life activities of growth, development, aging resistance and the like of the human body.

The edible Gelatin (Gelatin) is a hydrolysate of collagen, is a high protein without fat, does not contain cholesterol, and is a natural nutritional food thickener. After eating, people can not become fat, and the physical strength can not be reduced. Gelatin is also a strong protective colloid, has strong emulsifying power, and can inhibit coagulation of proteins such as milk and soybean milk due to gastric acid after entering stomach, thereby facilitating digestion of food. Gelatin is a macromolecular hydrocolloid, which is the product of partial hydrolysis of collagen. When edible gelatin is used as a coagulant, the bonding strength is mainly required.

The sweetening agent is one or more of sugar substitute, sugar, glucose syrup, high fructose syrup and the like. The sugar substitute is one or more of xylitol, sucralose, neotame, aspartame and acesulfame potassium; the edible sugar is one or more of white sugar, soft white sugar, yellow granulated sugar, brown granulated sugar, polycrystal crystal sugar, monocrystal crystal sugar, cubic sugar and borneose.

The menthol has mint fragrance and a cooling effect, and is commonly used as an aromatizer for toothpaste, perfume, beverage, candy and the like. Menthol is the main component of peppermint oil, is one of cyclic monoterpenes, is a high-volatility essential oil produced by plants, mainly contains hemiterpenes, monoterpenes and sesquiterpenes, has the effects of relieving itching, relieving pain, preventing corrosion, stimulating, anaesthetizing, cooling and resisting inflammation, and can be used for treating headache, neuralgia, pruritus, respiratory inflammation, atrophic rhinitis, hoarseness and the like.

Other raw materials in the invention are common raw materials in the prior art.

In the formula of the oral cavity helicobacter pylori resistant bead-blasting oral cavity fragrant pills, the liquorice and the tea polyphenol are bactericides and have the functions of inhibiting and killing HP and pathogenic bacteria. The probiotics and lactoferrin have the functions of resisting cancer, resisting microorganisms and oxidation and improving immunity. Xylitol, menthol, glycerol and the like are common raw materials in the prior chewing gum production and have the function of regulating the mouthfeel. As a result of the comprehensive effects of the components, the bead-blasting chewing gum has the functions of killing oral HP and other pathogenic microorganisms, keeping the oral cavity fresh and relieving halitosis.

The invention applies the principle of monarch, minister, assistant and guide of the traditional Chinese medicine, takes the traditional Chinese medicine plants with the continuous sterilization function as monarch drugs, takes the probiotics and the milk protein as minister drugs, and combines with the novel form of the bead-blasting chewing gum pills, makes full use of the buccal or chewing action in the oral cavity, leads the effective components to be rapidly absorbed by the human body through the oral mucosa and the blood brain barrier, thereby achieving the characteristics of restoring the normal micro-ecology of the oral cavity and killing the oral HP. Meanwhile, probiotic strains capable of accurately identifying HP are applied, and are attached to the surface of HP to form a copolymerization bacterial body by utilizing the principle of bacterium preparation by bacteria, and the copolymerization bacterial body falls off from the surface of the oral cavity and is discharged out of the human body during tooth brushing, so that the effect of reducing and controlling the quantity of HP in the oral cavity is achieved. The application of the probiotics can not cause HP to generate drug resistance, can widely inhibit the propagation and growth of gram-positive bacteria, gram-negative bacteria, yeast, fungi and the like, and antagonize the colonization of harmful bacteria such as HP and the like, thereby improving the oral flora distribution and restoring the normal micro-ecology of the oral cavity.

The bead-shaped chewing gum pill for resisting the oral cavity helicobacter pylori explosion has the advantages of quickly eliminating halitosis, keeping the oral cavity fresh for a long time, killing oral cavity HP and pathogenic bacteria, maintaining normal micro-ecology of the oral cavity and the like.

The bead chewing gum pill for resisting the oral cavity helicobacter pylori explosion has the following advantages:

1. the Chinese old liquorice is refined, and the glycyrrhizic acid, the flavone and other antioxidant substances have the characteristics of removing free radicals and natural bacteriostasis, replace clinical antibiotics and avoid the occurrence of antibiotic resistance.

2. The liquorice, the tea polyphenol, the probiotics and the milk protein can defend and tonify primordial qi of human bodies, such as heart qi, stomach qi, spleen qi and the like, improve the immunity and promote the health and micro-ecology of the human bodies.

3. The oral liquid passes through blood brain barrier through mucosa, has good absorption effect, has good inhibition and killing effect on oral cavity HP, and has obvious effect of relieving HP bacteria-derived halitosis.

4. Can effectively inhibit and remove dental plaque, reduce bacterial adhesion and aggregation, kill cariogenic bacteria, and prevent dental caries.

5. The chewing gum can keep fresh breath, can be chewed and swallowed, has no residue after chewing, and can keep the oral cavity fresh for a long time.

6. Is convenient to take and can be taken by various people.

Drawings

FIG. 1 shows 1: 2; 1: 4; the three concentration plates 1:8 all grow in a sterile manner and have bacteriostatic effects.

Detailed Description

The invention is further illustrated with reference to the following figures and specific examples. These examples are to be construed as merely illustrative and not limitative of the remainder of the disclosure in any way whatsoever. After reading the description of the invention, one skilled in the art can make various changes and modifications to the invention, and such equivalent changes and modifications also fall into the scope of the invention defined by the claims.

Example 1: preparation of bead-shaped chewing gum pill for resisting helicobacter pylori explosion in oral cavity

Weighing 20 kg of liquorice, 5 kg of tea polyphenol, 15 kg of probiotics (lactobacillus reuteri, lactobacillus acidophilus and animal bifidobacteria), 5 kg of cow milk protein, 10 kg of menthol, 3 kg of xylitol, 75 kg of gelatin, 15 kg of glycerol and 852 kg of vegetable oil.

In this embodiment, the wall material includes purified water, gelatin and glycerin, and the weight ratio is 2.0: 1.0: 0.5; the weight of the wall material accounts for 8-10% of the total weight of the bead-popping chewing gum. The preparation process comprises the following steps:

(1) adding purified water, glycerol, gelatin, etc. into a preparation kettle, heating to 30-45 deg.C, stirring uniformly for 45min, standing, and finishing preparation of external wall material;

(2) uniformly mixing raw materials such as liquorice, tea extracts, milk protein, menthol, xylitol and the like, and preparing a core material by probiotics:

(3) respectively introducing the prepared wall material and core material into a dripping pill machine at 30-45 deg.C, respectively introducing the wall material and core material into independent channels of concentric multi-layer drippers to obtain dripping pills, filtering, cleaning with purified water, and making into wet pills.

(4) Drying the wet pill to obtain dried blasting bead, cleaning with edible ethanol to remove vegetable oil and damaged blasting bead attached on surface, and air drying to obtain final product (water content: 8% -12%).

Example 2: preparation of bead-shaped chewing gum pill for resisting helicobacter pylori explosion in oral cavity

Weighing 1 kg of liquorice, 1 kg of tea polyphenol, 5 kg of probiotics (lactobacillus reuteri), 2 kg of cow milk protein, 1.4 kg of menthol, 0.1 kg of sucralose, 7.1 kg of gelatin, 0.9 kg of glycerol and 81.5 kg of vegetable oil for later use.

In this embodiment, the wall material includes water, gelatin and glycerin, and the weight ratio is 2.0: 1.0: 0.5; the weight of the wall material accounts for 8-10% of the total weight of the bead-popping chewing gum.

The preparation method is the same as example 1.

Test example 1

The anti-oral helicobacter pylori bead chewing gum pill prepared in the embodiment 1 of the invention is used for carrying out bacteriostasis tests. The specific test examples are as follows:

1. basis of experiment

According to the technical requirement of WS/T639-2018 antimicrobial drug sensitivity test of 4.2.1 agar dilution method, the bacteriostatic test is carried out.

2. Test method

2.1 preparing a helicobacter pylori culture medium with the concentration of 2 times, sterilizing and storing in an environment with the temperature of 55 ℃ for later use.

2.2 the anti-oral helicobacter pylori blasting bead chewing gum pill prepared in the example 1 contains 3000g of sample content of 2 percent of liquorice and is diluted in a gradient way according to the laboratory requirement, and the ratio is 1: 2; 1: 4; 1:8 for standby.

2.3 mixing the sample with culture medium at a ratio of 1:1, adding sterile defibrinated goat blood and helicobacter pylori additive, pouring into a flat plate, and solidifying for use.

2.4 the diluted bacterial suspension was inoculated onto a sample plate using a disposable 1. mu.L inoculating loop, 3. mu.L each, three sites in total, and the plate was left to dry at room temperature until the bacterial spot was dried. Continuously culturing for 5 days, and observing the growth condition of the lawn.

3. Evaluation of bacteriostatic Effect

And observing the growth condition of the colony at the inoculation point of the plate by naked eyes. If the bacteria-free colony grows, the concentration sample has the bacteriostatic action on the helicobacter pylori.

4. Test results

As shown in fig. 1, the experiment was designed for 1: 2; 1: 4; the three concentration plates 1:8 all grow in a sterile manner, namely, the three concentrations in the experimental design have the bacteriostatic effect.

3000g of sample contents containing 2% of liquorice in the anti-oral helicobacter pylori blasting bead chewing gum pills prepared in the example 2 are subjected to gradient dilution according to the laboratory requirements, and the ratio is 1: 2; 1: 4; 1:8, and carrying out the bacteriostasis test. The test results also show that 1: 2; 1: 4; the three concentration plates of 1:8 have bacteriostatic effects when all three concentrations of the bacteria-free colonies grow.

Test example 2

The anti-oral helicobacter pylori bead chewing gum pill prepared in the embodiment 2 of the invention is used for carrying out the test of relieving halitosis. The specific test examples are as follows:

1. evaluation method

The halitosis index is as follows: the measurements were performed by effective analytical instrumentation (breath gauge Halimeter Blu) using the main odor causing factor.

2. And (4) judging the standard:

after the statistical engineer calculates that 14 days later than the test product (anti-oral helicobacter pylori bead chewing gum) prepared in the example 2, the test group subjects take or chew 6g-12g per time 2-4 times per day, the halitosis index value after use is obviously lower than the value of the self baseline, and the difference between the halitosis index value of the test group subjects and the self baseline is obviously larger than the difference between the halitosis index value of the control group (only using placebo) after 14 days and the self baseline, and the anti-oral helicobacter pylori bead chewing gum is considered to have the effect of relieving halitosis.

Placebo is a chewing gum without active ingredients that reduce oral malodor.

3. Evaluation results

3.1 Point-to-Point comparison of time points

Note: mean (± standard error) rate of decline compared to baseline.

Note: the significance labeling method comprises the following steps: "n.s." means no statistical difference, p > 0.05; ". indicates there was a very significant difference, p > 0.05.

3.2 comparison of halitosis indicators between products

Note: mean value (± standard error)

Note: the significance labeling method comprises the following steps: "n.s." means no statistical difference p > 0.05; ". indicates significant differences, p > 0.05.

4. Conclusion of evaluation

Through clinical tests, 33 people in the test group use the test product (anti-helicobacter pylori blasting bead chewing gum pill) and test subjects in the test group take or chew 6g-12g pills each time according to 2-4 times per day. Compared with the baseline, the halitosis index of the subject after 14 days has significant difference, and the halitosis reducing effect is obvious. While the control group used only placebo, there was no significant difference between the bad breath indicator and the basal value in the subjects after 14 days.

The anti-oral helicobacter pylori bead chewing gum pill prepared in the embodiment 1 of the invention is used for carrying out the halitosis reducing test, and the test result also shows that the halitosis indexes of the subjects after 14 days are obviously different from the baseline, and the halitosis reducing effect is obvious.

The test results prove that: the anti-oral helicobacter pylori bead-bursting chewing gum pill has the effect of obviously relieving halitosis.

Claims (5)

1. The bead chewing gum pill for resisting oral helicobacter pylori explosion is characterized by comprising the following components in parts by weight:

0.1 to 2.5 portions of liquorice;

0.01-1 of tea polyphenol;

1-20 parts of probiotics;

0.1-5 of cow milk protein;

0.5-8 parts of menthol;

0.1-0.5 of sweetening agent;

6-8 parts of gelatin;

0.5-2 parts of glycerol;

edible vegetable oil added to 100 weight portions.

2. The anti-oral-cavity helicobacter pylori popping bead flavor pill as claimed in claim 1, wherein the probiotic bacteria are selected from the group consisting of lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium animalis, bifidobacterium lactis and lactobacillus rhamnosus.

3. The anti-oral helicobacter pylori popping bead chewing gum pellet as claimed in claim 1, wherein the sweetener is selected from the group consisting of sugar substitutes, sugar, glucose syrup and glucose-fructose syrup.

4. The anti-oral helicobacter pylori popping bead flavor pill as claimed in claim 3, wherein the sugar substitute is selected from the group consisting of xylitol, sucralose, neotame, aspartame and acesulfame k; the sugar is selected from white sugar, soft sugar, yellow sugar, brown sugar, polycrystal crystal sugar, monocrystal crystal sugar, cubic sugar and borneose.

5. A preparation method of a bead chewing gum pill for resisting oral helicobacter pylori explosion is characterized by comprising the following steps:

(1) preparing an external coating wall material: weighing glycerol and gelatin, placing the glycerol and the gelatin into a preparation kettle at 30-45 ℃, uniformly stirring, measuring the effective viscosity of 1000-1550cp, and standing for later use;

(2) preparing a core material: weighing liquorice, tea polyphenol, milk protein, menthol and a sweetening agent, uniformly mixing, and adding probiotics as a core material;

(3) respectively introducing the prepared wall material and core material into a dripping pill machine, dripping the wall material and the core material into edible vegetable oil at 30-45 deg.C, shrinking by concentric multi-layer drippers under the action of surface tension to obtain dripping pills, cooling and cleaning with purified water to obtain wet pills;

(4) drying the wet pills to obtain dry exploded beads, cleaning the dried exploded beads with edible ethanol to remove vegetable oil and damaged exploded beads attached to the surfaces, and air-drying to obtain the oral cavity helicobacter pylori explosion-resistant bead chewing gum pills with the water content of 8-12%.

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