KR20200017912A - Antibacterial Composition for Porphyromonas Gingivalis Comprising Plant Extract or Lactic Acid Bacteria Fermentation Product thereof - Google Patents

Abstract

The present invention relates to an antibacterial composition for Porphyromonas gingivalis containing a plant extract or a lactobacillus fermentation product thereof. More specifically, the present invention relates to: an antibacterial composition for Porphyromonas gingivalis; and a composition for preventing, alleviating or treating periodontal diseases, wherein compositions contain a plant extract of Avena sativa, green tea, Houttuynia cordata, and Centella asiatica, or a lactobacillus fermentation product, fermented by additionally adding lactobacillus to the same as an active component.

Description

Antibacterial Composition for Porphyromonas Gingivalis Comprising Plant Extract or Lactic Acid Bacteria Fermentation Product

The present invention relates to a composition for porphyromonas jinjivalis antimicrobial comprising a plant extract or a fermented lactic acid bacterium thereof, and more specifically, fermentation further comprising a plant extract of oats, green tea, fish vinegar and centella, or lactic acid bacteria thereto. The present invention relates to a composition for porphyromonas gingivalis antimicrobial, and a composition for preventing, improving or treating periodontal disease.

Healthy teeth are an important factor in determining the quality of life, which is said to be one of the five folds. Periodontal disease (periodontal disease) is one of the world's most prevalent diseases, which causes various clinical symptoms such as pain, masticatory dysfunction, periodontal tissue destruction, bad breath, and acute infection and causes tooth loss. It is known as a factor, and the cause of oral disease is increasing due to changes in diet.

Antibacterial is a method of suppressing the proliferation of oral pathogens that cause periodontal disease while living in plaque, and various kinds of antimicrobial agents including antibiotics having bactericidal and bacteriostatic effects on oral pathogens include caries, periodontal disease, pulp and root root infections. It has been developed as an inhibitor and therapeutic agent.

However, since antibiotics can cause the emergence and superinfection of resistant bacteria in the oral cavity with systemic side effects on our body, long-term use is difficult and can be used only as a therapeutic agent.

Under this background, the present inventors based on some of the active ingredients and compositions of the present inventors preventive and therapeutic composition developed by the present inventors (Korean Patent No. 10-1771479), it is used for the treatment of oral diseases such as periodontal disease As a result of conducting a study for the development of the formulation, the present invention was completed by confirming that it has an antimicrobial effect on Porphyromonas gingivalis causing the periodontal disease.

One object of the present invention is to provide an antimicrobial composition for Porphyromonas Gingivalis comprising oat, green tea, fish herb and plant extract of Centella as an active ingredient.

Another object of the present invention is to provide a composition for porphyromonas ginjivalis antimicrobial, further comprising lactic acid bacteria in the plant extract.

Another object of the present invention to provide a pharmaceutical composition and quasi-drug composition for preventing, improving or treating periodontal disease using the antimicrobial composition.

In order to achieve the above object, one embodiment of the present invention provides an antimicrobial composition of Porphyromonas Gingivalis comprising oat, green tea, fish herb and plant extract of Centella as an active ingredient.

In addition, it provides porphyromonas jinjivalis antimicrobial composition further comprising lactic acid bacteria in the plant extract.

In the present invention, while searching for plants having antimicrobial efficacy, in particular, antibacterial efficacy against Porphyromonas jinjivalis among various plants, oat, green tea, eochocho and Centella as expected to be able to exert the excellent efficacy of these mixed plants The extract was configured, and based on this, the antimicrobial efficacy was confirmed, thereby confirming the excellent antimicrobial efficacy, thereby completing the present invention. In particular, by combining the lactic acid bacteria in addition to the mixed plant extract, it was confirmed that the antimicrobial activity is more pronounced when culturing the lactic acid bacteria fermented product. In addition, as a result of experiments by properly adjusting the blending ratio of the mixed plant extract and lactic acid bacteria, it was confirmed that the optimum antimicrobial activity was exhibited when the ratio of the lactic acid bacteria and plant extracts is 2: 1.

In the present invention, the "plant extract" may be used in combination with the "mixed plant extract", and may include extracts of oats, green tea, edible herbs and centella, and specifically may include the same amount thereof. The plant extract may further include a plant having another kind of antimicrobial activity, and for example, may further include aloe vera, lamiaceae, Astragalus, red ginseng, mugwort leaves, ducts, ginger, and cherries.

In the present invention, oat (Avaena sativa L.) is a perennial herbaceous plant belonging to the family Asteraceae, and is a crop also called oat or wheat. It is known to be good for nail and skin health.

Green tea (green tea) in the present invention, a perennial evergreen arborescent growing in a relatively warm place, the catechin component of astringent taste inhibits the increase in blood pressure and acts to block the activity of the cold virus, digestion of sugar It delays absorption, preventing blood pressure rise and blood sugar level increase, thus preventing diabetes and high blood pressure.

In the present invention, the herbaceous herb (Heartleaf Houttuynia, Houttuynia cordata) is a perennial herb belonging to over three hundred called wild buckwheat. It contains abundant ingredients such as flavonoids, quercitrin, myrsen and laurylaldehyde, which are effective for diuretic and skin care.

As the perennial herbaceous plant (Asiatic pennywort), as a perennial herb, containing Asianticoside (Asiaticoside), Asiantic acid (Asiatic acid), madecassic acid (madecassic acid) to control and promote collagen synthesis, elasticity and skin Gives health

However, the oats, green tea, eoseongcho and centella are not known for their antimicrobial efficacy, specifically, the antimicrobial efficacy against Porphyromonas jinjivalis, which was confirmed for the first time in the present invention.

In the present invention, porphyromonas ginjivalis antimicrobial composition may further comprise lactic acid bacteria. Specifically, by combining the plant extract and lactic acid bacteria can be maximized antibacterial effect.

The lactic acid bacteria is also called lactic acid bacteria, and generically refers to posterior wall fungus bacteria that break down carbohydrates into lactic acid by metabolism. Dairy products such as yoghurt, yoghurt, and cheese, brewed foods such as sake, soybean paste, and soy sauce and kimchi are fermented by lactic acid to prevent the growth of pathogens and harmful bacteria. Within the organs or vagina, it protects the body from other pathogens and helps maintain homeostasis.

In the present invention, by appropriately blending the lactic acid bacteria with plant extracts and culturing them, the lactic acid bacteria fermented product of the plant extracts was prepared by allowing the plant extracts to ferment by lactic acid bacteria. In addition, it was confirmed that the lactic acid bacteria fermented product has excellent porphyromonas jinjivalis antibacterial effect. The lactic acid bacteria fermented product is to collectively reach the fermented material using lactic acid bacteria.

The lactic acid bacteria may be appropriately used strains of the genus Lactobacillus, but is not limited thereto, and specifically, may be used Lactobacillus rhamnosus or Lactobacillus plantarum. In one embodiment of the present invention using Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) was confirmed the antimicrobial effect.

In the present invention, there is an additional feature to confirm the appropriate blending ratio of the lactic acid bacteria and plant extracts for antimicrobial efficacy. Specifically, it may be most preferable that lactic acid bacteria and plant extracts are included in a weight ratio of 2: 1. In one embodiment of the present invention, the results of experiments by combining the lactic acid bacteria and plant extracts in various ratios (Table 1), when the lactic acid bacteria and plant extracts are included in the weight ratio of 2: 1, the best porphyromonas ginjivalis antimicrobial efficacy It was confirmed to have (Fig. 7).

As another aspect, the present invention provides a pharmaceutical composition for preventing or treating periodontal disease, comprising as an active ingredient plant extracts of oats, green tea, eochocho and centella. The present invention also provides a pharmaceutical composition for preventing or treating periodontal disease, which further comprises lactic acid bacteria in the plant extract.

As yet another aspect, the present invention provides a quasi-drug composition for preventing or improving periodontal disease, comprising as an active ingredient plant extracts of oats, green tea, eochocho and centella. The present invention also provides a quasi-drug composition for preventing or improving periodontal disease, further comprising lactic acid bacteria in the plant extract.

In the present invention, as described above for the plant extract or fermented lactic acid bacterium thereof, the composition of the present invention may have an antimicrobial effect against Porphyromonas ginjivalis may be effective in preventing, improving or treating periodontal disease.

In the present invention, "periodontal disease" is a disease in which the gingival, periodontal ligament and bone tissue supporting the tooth are inflamed, which is also commonly referred to as flavour, and as gingivitis and periodontitis depending on the degree of the disease Divided. It is a relatively mild and fast-recovering form of periodontal disease, namely gingivitis, which is limited to gums, or soft tissues, and this type of inflammation is called periodontitis.

There are several types of bacteria that cause periodontal disease, such as Porphyromonas ginjivalis. Porphyromonas gingivalis is a kind of bacteroid (Bacteroide) flexible bacteria, gram-negative bacteria, anaerobic bacteria. The porphyromonas gingivalis is found in the oral cavity in which periodontal disease has occurred, as well as in the upper gastrointestinal tract, respiratory tract and colon. Accordingly, it is possible to prevent, ameliorate or treat periodontal disease through the antibacterial activity against Porphyromonas gingivalis.

The pharmaceutical composition of the present invention in the form of tablets, pills, powders, granules, capsules, suspensions, solvents, emulsions, syrups, aerosols, sterile injectable solutions and the like according to conventional methods for preventing and treating oral diseases Formulation is possible. In addition, the pharmaceutical compositions of the present invention may further comprise a carrier, excipient or diluent. The specific dosage of the pharmaceutical composition according to the present invention depends on factors such as the method of formulation, the condition and weight of the patient, the sex, age, extent of disease, drug form, route and duration of administration, rate of excretion, response sensitivity, etc. Various choices can be made by those skilled in the art, and the dosage and frequency do not limit the scope of the present invention in any aspect.

The quasi-drug composition of the present invention may include an oral quasi-drug. Ingredients included in the quasi-drug composition of the present invention may include components commonly used in the quasi-drug composition for oral cavity as an active ingredient, for example, abrasives, wetting agents, binders, foaming agents, sweeteners, preservatives, medicinal ingredients, flavors Agents, dyes, solvents, brighteners, solubilizers or pH adjusters.

Oral quasi-drug composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, oral varnish It may have a formulation such as mouthwash, gum massage cream, but is not limited thereto.

The plant extract of the present invention and its lactic acid bacteria fermented product have antimicrobial activity against Porphyromonas gingivalis and can be applied to various types of products for the improvement and treatment of periodontal disease.

1 to 8 show the results of the antimicrobial activity (Disk diffusion test) for Porphyromonas jinjivalis.
Specifically, FIG. 1 is a negative control, FIG. 2 is a positive control, FIG. 3 is an experimental group A, FIG. 4 is an experimental group B, FIG. 5 is an experimental group C, FIG. 6 is an experimental group D, FIG. 7 is an experimental group E, and FIG. 8 is an experimental group F It is a result of measurement of antibacterial activity.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example 1: Material Preparation

Porphyromonas Gingivalis strain, which is an antimicrobial activity, was purchased from the Korea Institute of Bioscience and Biotechnology (KCTC) (strain number 272572).

The lyophilized Porphyromonas ginjivalis was cut into slotted ampoules using a glass cutting knife, cut, and suspended by adding 0.5 ml of sterile distilled water. 200 μl of the suspended sample was collected and transferred to 200 ml of Yeast Mold (YM) Broth liquid medium, and a small amount was taken with a disposable smear rod and transferred to Yeast Mold (YM) Agar solid medium for inoculation. The medium inoculated with Porphyromonas gingivalis was incubated in a 37 ° C shaking incubator. After 24 hours, the bacteria of YM Agar solid medium were identified by gram staining, which is a bacterial differentiation method. Thereafter, the bacteria were activated through multiple passages and used for experiments.

In addition, a total of four plant extracts of oat extract, green tea extract, eochocho extract and centella extract and Lactobacillus rhamnosus (lactobacillus rhamnosus) were prepared to measure the antibacterial activity of Porphyromonas gingivalis.

Example 2: Porphyromonas ginjivalis antibacterial activity (Disk diffusion test)

Agar medium diffusion was performed to determine the antimicrobial activity of each test substance against Porphyromonas jinjivalis. Specifically, 100 μl of the bacterium diluted with Porphyromonas jinjivalis to YM liquid medium at 10 ⁷ CFU / ml was dropped into YM solid medium, and dried for 5-10 minutes after smearing using a sterilized disposable spreader. Each control and experimental group was semi-dried by dropping each control and experimental groups on a sterile 6 mm paper disc, and then placed on YM solid medium on which test strains were smeared. Thereafter, the plant extract was fermented with lactic acid bacteria while incubated for 24 hours in a shaking incubator at 37 ° C. Then, the resulting clear zone (clear zone) around the paper disc was observed.

The blending ratio of the lactic acid bacteria and four plant extracts included in each experimental group is shown in Table 1 below.

Experimental group Lactobacillus: 4 plant extracts A 1: 1 B 1: 2 C 1: 5 D 1: 10 E 2: 1 F 5: 1

A control group and an experimental group were added to the sterilized paper disc, and then dried completely and the transparent ring was observed. After inoculation three times for each experimental group, the result was observed after 24 hours, and the transparent ring formed around the paper disc was observed.

As a result, no clear ring was observed in the negative control group treated with the sample (FIG. 1), and a clear ring was widely observed in the ethanol positive control group known to have antimicrobial activity (FIG. 2).

In addition, by confirming that the transparent ring is observed in the experimental group treated with a mixture of lactic acid bacteria and four plant extracts (FIG. 3 to 8), it was confirmed that the mixed cultures with four plant extracts, further lactic acid bacteria has an antibacterial effect.

On the other hand, in particular, by confirming that the widest transparent ring was observed in the experimental group E having a combination ratio of lactic acid bacteria and four plant extracts 2: 1 (Fig. 7), it was confirmed that the antibacterial activity against Porphyromonas jinjivalis is the best.

Accordingly, it can be seen that the four plant extracts of the present invention, in particular, the fermented lactic acid bacteria fermented by mixing them with lactic acid bacteria, have an antimicrobial effect against Porphyromonas jinjivalis and oral diseases such as periodontal disease. there was.

From the above description, those skilled in the art will appreciate that the present application can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. The scope of the present application should be construed that all changes or modifications derived from the meaning and scope of the following claims and equivalent concepts rather than the detailed description are included in the scope of the present application.

Claims (9)

Porphyromonas Gingivalis antimicrobial composition comprising plant extracts of oats, green tea, eochochocho and Centella as an active ingredient.
The antimicrobial composition of claim 1, wherein the composition further comprises lactic acid bacteria.
The antimicrobial composition of claim 2, wherein the plant extract is fermented by lactic acid bacteria.
The antimicrobial composition of claim 2, wherein the lactic acid bacteria is Lactobacillus rhamnosus .
The antimicrobial composition of claim 2, wherein the lactic acid bacteria and plant extracts are included in a weight ratio of 2: 1.
Oat, green tea, eoseongcho and medicinal herbs containing plant extracts as an active ingredient, periodontal disease prevention or treatment pharmaceutical composition.
The pharmaceutical composition of claim 6, wherein the composition further comprises lactic acid bacteria.
Oval, green tea, eoseongcho and medicinal herbs containing plant extracts as an active ingredient, periodontal disease prevention or improvement of quasi-drug composition.
The quasi-drug composition according to claim 8, wherein the composition further comprises lactic acid bacteria.

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