KR20130097537A - Composition comprising mixture of hot water extract of curcuma longa radix, acorus gramineus soland and polygala tenuifolia to enhance cognition and memory - Google Patents
Composition comprising mixture of hot water extract of curcuma longa radix, acorus gramineus soland and polygala tenuifolia to enhance cognition and memory Download PDFInfo
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- KR20130097537A KR20130097537A KR1020120019268A KR20120019268A KR20130097537A KR 20130097537 A KR20130097537 A KR 20130097537A KR 1020120019268 A KR1020120019268 A KR 1020120019268A KR 20120019268 A KR20120019268 A KR 20120019268A KR 20130097537 A KR20130097537 A KR 20130097537A
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- hot water
- turmeric
- mixture
- water extract
- seokchangpo
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/69—Polygalaceae (Milkwort family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/882—Acoraceae (Calamus family), e.g. sweetflag or Acorus calamus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/322—Foods, ingredients or supplements having a functional effect on health having an effect on the health of the nervous system or on mental function
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
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Abstract
The present invention relates to a composition for improving cognition and memory including turmeric, stone Changpo and hot water extract of raw paper as an active ingredient. Turmeric, Seokchang-po and hot water extracts of raw paper according to the present invention exhibit excellent antioxidant, AchE inhibitory effect, and ACE inhibitory effect, which can be useful for improving cognition and memory, and are useful for medicines and health for Alzheimer's dementia. Can be used as food.
Description
The present invention relates to a composition for improving cognition and memory, including turmeric, stone Changpo, and hot water extract of raw paper as an active ingredient.
In modern society, due to environmental pollution caused by urbanization and industrialization, excessive stress, drinking and smoking, excess free radicals are produced not only in the brain but also in each tissue and organ. Active oxygen refers to oxygen radicals and oxygen compounds derived from oxygen radicals, and includes superoxide anion radicals, hydroxyl radicals, oxygen radicals, hydrogen peroxide, and singlet oxygen. Free radicals damage the human body by damaging DNA, cell constituent proteins, and lipids in living cells. If the damage is not repaired and accumulated, it causes diseases such as brain disease, aging, cancer, and heart disease. In particular, harmful active oxygen, including hydrogen peroxide, superoxide anion, and hydroxyl radicals, which are inevitably generated in the process of using oxygen in vivo, is excessively generated as amyloid beta proteins accumulate in brain cells, resulting in DNA damage, oxidized proteins, and Increasing lipid peroxide leads to cell dysfunction and disruption of membrane fluidity, which in turn causes cell death, leading to decreased cognition and memory. The human body has an antioxidant system such as superoxide dismutase (SOD), catalase, tocopherol, ascorbic acid, carotenoids, glutathione, etc. as a self-defense mechanism against free radicals, but since it is very small, it is necessary to supplement supplements with antioxidants. There is.
On the other hand, as the birth rate decreases recently, as the elderly population increases due to the development of medicine and the aging socialization accelerates, senile diseases such as cognitive and memory deterioration are emerging as social problems. Among them, dementia is rapidly increasing. The types of dementia include cerebrovascular dementia caused by degenerative change, cerebral infarction, and addictive dementia. The pathogenesis of Alzheimer's disease, which accounts for 50% to 60% of the majority of people with dementia, results in the production of high levels of free radicals in brain cells, resulting in oxidative stress altering the structure and function of intracellular mitochondria, leading to brain disease. It is known to become. The main symptoms of Alzheimer's are memory loss and cognitive impairment, presumably due to severe damage to the cholinergic system, which plays an important role in memory and learning. It is reported that as the damage of acetylcholine-producing neurons increases, the increase of acetylcholinesterase (AChE) activity, which degrades acetylcholine, worsens memory loss and cognitive impairment in Alzheimer's patients. Therefore, AChE inhibitors that inhibit the activity of cholinergic agonists, acetylcholine precursors, and AChE, which activate the action of the neurotransmitter acetylcholine, in order to treat or alleviate symptoms of Alzheimer's by activating the cholinergic action of the cerebral nerve junctions. Much research has been carried out on. In particular, clinically commercialized drug Tacrine acts to maintain cognitive ability by temporarily inhibiting AChE activity, but serious side effects such as liver disorders due to toxicity caused by long-term use, gastrointestinal disorders such as abdominal pain and diarrhea There is a problem that there is.
Therefore, in order to solve such a problem, in recent years, in order to develop prescriptions with low toxicity, various studies on brain disease have been conducted based on related causal theories. In other words, the study on the antioxidant activity that causes aging in association with aging of the brain using mono-drugs and complex prescriptions, and the effects on the memory and behavior of the white paper and TNF, an inflammatory cell activator from cerebral astrocytes Inhibition of the production of -α (tumor necrosis factor-α), IL-1 and Ab has been studied. Recently, herbal medicines in the overexpressed cell lines of amyloid proprotein and presenilin gene, which are the cause of dementia, have been studied. The results of the study have been reported. Many remedies are known to improve learning and memory, which may be related to nervous system excitability, improved brain microcirculation, and increased blood flow to the brain, as well as increased protein synthesis and cerebral development. It also seems to contribute to improving learning. Related Korean Patent Publication Nos. 10-0564904, 10-0382564, 10-0360674, and the like are known.
However, the pharmacological agents introduced above have limitations in preventing and treating the deterioration of brain function such as cognition and memory, and also contain too many kinds of herbal extracts, so there is a possibility that various side effects occur in the long term use. have. In addition, since most of the memory improvers through natural products are less effective than synthetic drugs AChE inhibitors, the development and discovery of medicinal agents with excellent efficacy and safety are urgently required.
The inventors of the herbal medicine has fewer side effects, excellent cognition and memory improvement effect, and while researching to search for useful substances for the prevention and treatment of brain function degradation, antioxidant effect, when using a combination of turmeric, Seokchangpo, raw water extract It was confirmed that the ACE activity inhibitory effect and the AChE activity inhibitory effect were excellent, and completed the present invention.
Therefore, the present invention is to provide a composition for improving cognition and memory, including turmeric, stone Changpo, hot water extract of the base paper as an active ingredient.
The present invention provides a composition for improving cognition and memory, comprising a mixture of turmeric, Seokchangpo and hot water extracts of raw paper as an active ingredient.
Turmeric, Seokchang-po and hot-water extracts of raw paper according to the present invention exhibit excellent antioxidant, ACE, and AChE activity inhibitory effects, and thus may be useful for improving cognition and memory.
1 is a view showing the reducing power of the mixture of turmeric, Seokchangpo and hot water extract of the base of the present invention.
Figure 2 is a diagram showing the superoxide (superoxide) scavenging ability of the mixture of turmeric, Seokchangpo and hot water extract of raw paper of the present invention.
Figure 3 is a diagram showing the hydroxyl radical (Hydroxyl radical) scavenging ability of the mixture of turmeric, Seokchangpo and hot water extract of the base of the present invention.
Figure 4 is a diagram showing the hydrogen peroxide scavenging ability of the mixture of turmeric, Seokchangpo and hot water extract of the base paper of the present invention.
Figure 5 is a diagram showing the toxicity through the MTT analysis of the cells of the mixture of turmeric, Seokchangpo and hot water extract of the present invention.
Figure 6 is a diagram showing the effect of inhibiting DNA oxidation by the hydroxyl radical (Hydroxyl radical) of the mixture of turmeric, Seokchangpo and hot water extract of raw paper of the present invention.
Figure 7 is a view showing the inhibitory effect of the mixture of turmeric, Seokchangpo and raw paper hot water extract of the present invention for ACE (angiotensin converting enzyme).
Figure 8 is a view showing the inhibitory effect of the mixture of turmeric, Seokchangpo and raw paper hot water extract of the present invention on AChE (acetylcholine esterase).
The present invention provides a composition for improving cognition and memory, comprising a mixture of turmeric, Seokchangpo and hot water extracts of raw paper as an active ingredient.
The composition includes a pharmaceutical composition and a food composition.
Hereinafter, the present invention will be described in detail.
Turmeric as an active ingredient in the composition of the present invention is dried root of Curcuma longa L., a perennial herbaceous root belonging to the family of Gingeraceae. ), It has been known to have the effect of septic gyeonggi (破血 行 氣), painful pain (通 經 止痛). Curcumin, the main ingredient, is a substance that makes turmeric yellow, and many studies have been published as it has been found to have anti-cancer effects including anti-inflammatory and antioxidant effects.
Acorus gramineus Soland, an active ingredient in the composition of the present invention, is a dried root of Acorus gramineus soland, a perennial herb belonging to the genus Araceae, and the related plant, which is used for medicinal purposes. The main components of the rhizome are 2 to 3% of volatile essential oils, and the aromatic components include pinen, camphor, calmen and calmenol. Other glycosides of erolin, calamine and choline Contains alkaloids Seokchangpo clears the mind, circulates blood well, removes wind and wind, and specifically acts on the liver, spleen, and heart. It has the pharmacological effects of soothing, healthy, analgesic, diuretic, and antifungal. It is used for febrile illnesses such as consciousness disorder, anxiety, anxiety, tightness of breath, hot flashes, nystagmus, duhun, and hearing loss, and it is known that fresh products have a strong effect on high temperature consciousness disorder. In addition, it has been known that the smooth muscle haeparoscope function to promote the secretion of digestive fluid during oral administration, to inhibit the abnormal fermentation of the stomach and to relax the intestinal smooth muscle.
The base paper (遠志, Polygala tenuifolia) as an active ingredient in the composition of the present invention is a perennial herb of the dicotyledonous rats and rats, and grows well in sunny places and has a height of about 30 cm. Roots are thick and long, with several main stems coming from the ends and almost without hairs. In oriental medicine, the root is called Wonji and it is used as expectorant, tonic and gangjeong. It is distributed in the Yellow Sea, North Hamgyong Province, and northern China. The temper of the earth is recorded as high, divine, warm, non-toxic, and returning to the heart, lungs, and god. The ground helps wisdom, brightens ears and eyes, strengthens the will, calms the heart, soothing heart palpitations, stabilizes the mind as well as keeps the mind from being blurred, leaves replenishes energy, leaves weakness and dreaminess.夢 精) is known to stop things.
Turmeric, stone spear cloth, and raw paper hot water extract of the present invention can be obtained by a conventional hot water extraction method, or a commercially available one can be purchased and used.
In the present invention can be obtained by extracting turmeric, stone Changpo, raw paper hydrothermal extract in the following method. First, turmeric, Seokchangpo, and raw paper are washed thoroughly with running water, and then naturally dried. The washed and dried herbal medicines are mixed with distilled water and a weight (g) ratio of 1:10 to 50, respectively, and the extract of moist heat is repeated 1 to 5 times at 70 to 90 ° C. for 4 to 5 hours with a shaker. After filtering the extract, the filtered filtrate is lyophilized to obtain a powder of turmeric, stone Changpo, raw paper hot water extract. Turmeric hot water extract, Seokchangpo hot water extract, and raw paper hot water extract of each powder form are mixed in a weight ratio of 0.1-10: 0.1-10: 0.1-10, and then mixed with a phosphate buffer solution to obtain hot water extracts of turmeric, Seokpo, and raw paper Prepare the mixture.
Turmeric, Seokpo, and raw hot water extracts of the present invention have a very high concentration of reducing power, superoxide scavenging ability, hydroxyl radical scavenging ability, and hydrogen peroxide scavenging ability, compared to turmeric, stone changpo, and raw hot water extract alone. Oxidative damage of DNA is significantly inhibited.
In addition, turmeric, Seokchangpo, and raw hot water extracts of the present invention do not show cytotoxicity and are safe in effective concentrations, and have an excellent effect of inhibiting ACE activity and inhibiting AChE activity.
Therefore, the turmeric, Seokchangpo, and raw hot water extract according to the present invention exhibits an antioxidant effect, an ACE inhibitory effect, and an AChE activity inhibitory effect, and thus may be useful for improving cognition and memory.
In general, the main symptom of senile degenerative brain disease, especially Alzheimer's disease, is deterioration of cognition and memory, and Alzheimer's disease is damaged by choline system due to active oxygen damage and the effect of AchE. It is known to develop.
Therefore, turmeric, stone Changpo, raw hot water extract of the present invention can be used as a medicine and health food useful for Alzheimer's dementia.
The composition of the present invention may include one or more known active ingredients having a cognitive and memory-improving effect with a mixture of turmeric, stone spear, and hot water extracts of raw paper.
The composition of the present invention may further comprise at least one pharmaceutically acceptable carrier in addition to the above-described effective ingredients for administration. Pharmaceutically acceptable carriers may be used in combination with saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol and one or more of these components, if necessary, as an antioxidant, buffer And other conventional additives such as bacteriostatic agents can be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Further, it can be suitably formulated according to each disease or ingredient, using appropriate methods in the art or by the method disclosed in Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA.
The composition of the present invention can be administered orally or parenterally (eg, applied intravenously, subcutaneously, intraperitoneally or topically) according to the desired method, and the dosage is based on the weight, age, sex and health of the patient. The range varies depending on the diet, the time of administration, the method of administration, the rate of excretion and the severity of the disease. The daily dose of the turmeric, Seokchangpo, and hot water extract of raw paper is about 1 to 1000 mg / kg, preferably about 10 to 100 mg / kg, preferably administered once to several times a day.
The composition of the present invention can be used alone or in combination with methods using surgery, hormonal therapy, drug therapy and biological response modifiers to improve cognition and memory.
In addition, the composition of the present invention can be added to health food for the purpose of preventing and improving the improvement of cognition and memory. When using the mixture of turmeric, stone spear, and raw hot water extract of the present invention as a food additive, the mixture of turmeric, spearmint, raw hot water extract can be added as it is or used with other foods or food ingredients, and according to conventional methods Can be used. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). In general, in the preparation of food or beverages, the mixture of turmeric, quarry and raw hydrothermal extract of the present invention is added in an amount of 15% by weight or less, preferably 10% by weight or less based on the raw material. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .
There is no particular limitation on the kind of the food. Examples of the food to which the substance may be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, dairy products including other noodles, gum, ice cream, various soups, beverages, tea, drink, Alcoholic beverages and vitamin complexes, and the like and include all of the health foods in the conventional sense.
The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. The above-mentioned natural carbohydrates are glucose, monosaccharides such as fructose, maltose, disaccharides such as sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.
In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, And carbonating agents used in the carbonated beverage. In addition, the composition of the present invention may include a pulp for the production of natural fruit juice, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.
Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.
< Example 1> curcuma , Seokchangpo , Original Of hot-water extract Preparation of mixtures
Turmeric, Seokchangpo, and raw paper used in the present invention were washed three times in each flowing water and then naturally dried. Then, 100 g of each of the washed and dried herbal medicines (turmeric, stone-changpo, raw paper) was mixed with 3L of distilled water, and then shaken with a shaker. Wet heat extraction for 5 hours at ℃ (3 times). Each extract was filtered and the filtered filtrate was lyophilized to obtain hot water extracts in powder form (turmeric hot water extract: 7.2 g, quarry stalk hot water extract: 6.4 g, raw paper hot water extract: 5.3 g). The powdered hot water extract alone or the mixture of turmeric hot water extract, Seokchangpo hot water extract, and raw paper hot water extract in a weight ratio of 1: 1: 1 was mixed with a phosphate buffer solution and then 0.00625, 0.0125, 0.025, 0.05, 0.1%, respectively. It was prepared in concentration and used in the following experiment.
< Experimental Example 1> curcuma , Seokchangpo , Paper Of hot-water extract Reducing power analysis of the mixture
Oyaizu (Oyaizu, M. 1986. Studies on products of browning reactions: ntioxidative activities of products of browning reaction prepared from glucosamine.Japanese Journal of Nutrition 44, 307-315.). Specifically, 1 ml of the sample prepared in Example 1 was added to each of 1 ml of 200 mM phosphate buffer of pH 6.6 and 1 ml of potassium ferricyanide solution in order, followed by stirring, followed by 20 minutes in a 50 ° C. water bath. Reacted for a while. 1 ml of 10% TCA (trichloroacetic acid) solution was added thereto, followed by centrifugation at 13,500 X g for 15 minutes to obtain a supernatant. 1 mL of the supernatant was mixed with 1 mL of distilled water and ferric chloride, and the absorbance was measured at 700 nm with a spectrophotometer. The reducing power of the sample was converted into% value of the absorbance ratio between the sample addition group and the control group. The data were obtained as the mean value ± standard deviation of the results of three experiments, the reliability was given statistically using the Student's test. Ascorbic acid (vitamin C) was used as a positive control.
The results are shown in Fig.
As shown in Figure 1, the turmeric, stone Changpo, raw paper hot water extract of the present invention was treated by each concentration (0.00625, 0.0125, 0.025, 0.05, 0.1%) as a result, each turmeric, stone Changpo, raw hot water extract alone It was confirmed that the reducing power is more excellent. In particular, turmeric, Seokchangpo, and raw hot water extracts were found to be significantly better than the positive control group (vitamin C) at concentrations of 0.05% or more (*, P <0.05; **, P <0.01).
< Experimental Example 2> curcuma , Seokchangpo , Paper Of hot-water extract Superoxide of the mixture Superoxide ) Scatters analysis
In order to measure the degree of antioxidant activity by scavenging superoxide, the superoxide produced in the process of converting hypoxathine to uric acid by Xanthine oxidase is NBT ( nitroblue tetrazolium). Specifically, in 400 μl of 50 mM potassium phosphate buffer (pH 7.4) containing 0.6 mM hypoxanthine, 1 mM EDTA, and 0.2 mM NBT, a mixture of turmeric, stone spear, and hot water extracts prepared in Example 1 was used. 0.00625, 0.0125, 0.025, 0.05, 0.1%) were added and mixed by 5 μl, respectively. The reaction was started by adding 100 μl of xanthine oxidase (100 mU / ml). The reaction mixture was incubated at 37 ° C. for 20 minutes and then subdivided into 200 μl portions in 96 well plates, and the absorbance was measured at 590 nm using a
The results are shown in Fig.
As shown in Figure 2, all the experimental groups treated with the mixture of turmeric, Seokchangpo, raw paper hot water extract of the present invention was confirmed that as the concentration increases, the superoxide scavenging effect increases. Among them, it was confirmed that the superoxide scavenging effect of the mixture of turmeric, Seokchangpo and hot water extracts of the present invention was significantly best (*, P <0.05).
< Experimental Example 3> curcuma , Seokchangpo , Paper Of hot-water extract Mixture of Hydroxyl Radical (Hydroxyl radical ) Scatters analysis
In order to measure the degree of antioxidant activity by scavenging hydroxyl radicals, the experimental method of Chung et al. (Yang, Y., Kim, Y. and Chung, H. Peroxynitrite and Hydroxyl Radical Scavenging Activity of Dihydroxybenzaldehydes.) Was modified. The hydroxyl radical was measured. Specifically, hydroxyl radicals were generated by Fenton reaction using 50 µl of 10 mM and 50 µl of 10 mM. The generated radicals were prepared in Example 1 in the presence of 25 μl of 10 mM EDTA, 25 μl of 10 mM 2-deoxyribose, 150 μl of 0.1 M phosphate buffer (pH 7.4). The hot water extract mixture was mixed by concentration (0.00625, 0.0125, 0.025, 0.05, 0.1%), and then reacted at 37 ° C. for 4 hours. 250 μl of 2.8% TCA (trichloroacetic acid) and 250 μl of 1% TBA (thiobabituric acid) were added to the reaction mixture, followed by heating to 100 ° C. The heated reaction solution was cooled to room temperature and then centrifuged at 1,000 X g for 5 minutes. The collected supernatant was measured for absorbance at 532 nm using a spectrophotometer, and the antioxidant activity was converted into% by the absorbance ratio before and after sample addition. Ascorbic acid (vitamin C) was used as a positive control.
The results are shown in FIG.
As shown in FIG. 3, the positive control was found to have no scavenging effect on hydroxyl radicals, and rather increased production of hydroxyl radicals. The treatment of turmeric and raw paper hydrothermal extracts alone did not show hydroxyl radical scavenging effects, but the spearmint hydrothermal extracts had scavenging effects on hydroxyl radicals at concentrations of 0.05% or more. Turmeric, Seokchangpo, and hot water extracts of raw paper of the present invention were found to increase the scavenging effect on hydroxyl radicals in a concentration-dependent manner (*, P <0.05; **, P <0.01).
< Experimental Example 4> curcuma , Seokchangpo , Paper Of hot-water extract Hydrogen Peroxide in Mixture Scatters analysis
In order to measure the antioxidant effect through the scavenging of hydrogen peroxide in the mixture of turmeric, Seokchangpo, and hot water extracts of the present invention, Choi et al. (Choi, C., Kim, S., Hwang, S., Choi, B., Ahn, H., Lee, M., Park, S. and Kim, S. 2002.Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison.Plant science (Limerick) 163, 1161-1168 Hydrogen peroxide was measured by modifying the experimental method of. Specifically, 20 µl of 10 mM hydrogen peroxide and 100 µl of 0.01 M phosphate buffer solution (pH5.0) were added to 80 µl of the mixture of turmeric, stone Changpo, and raw hot water extract prepared in Example 1, at 37 ° C. The reaction was carried out for 5 minutes. Then, 15 μl of 1.25 mM ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) and 30 μl of peroxidase were added to react at 37 ° C. for 10 minutes. Absorbance was measured at 405 nm with a spectrophotometer. For antioxidant activity, the absorbance ratio before and after sample addition was converted into% value. Ascorbic acid (vitamin C) was used as a positive control.
The results are shown in Fig.
As shown in Figure 4, the positive control showed an antioxidant effect of 50%, the mixture of turmeric, Seokchangpo, raw paper of the hot water extract of the present invention showed that the hydrogen peroxide scavenging effect at the concentration of 0.05% or more significantly the best. It was confirmed (*, P <0.05).
< Experimental Example 5> curcuma , Seokchangpo , Paper Of hot-water extract Cytotoxicity Measurement of Mixtures- MTT analysis
In order to measure the toxicity and concentration of turmeric, Seokchangpo, raw hot water extract mixture of the present invention on the cells, the following experiment was performed.
1. Incubation of PC12 Cells
PC12 cells were treated with 10% fetal bovine serum, 2 mM glutamine and 100 μg / ml penicillin-streptomycin in an incubator maintained at 95% or higher humidity at 5% and 37 ° C. Cultured in DMEM (Dulbecco's Modified Eagle's Medium) containing.
2. MTT Assay
Hansen (Hansen, M., Nielsen, S., Berg, K., 1989. Re-examination and further development of a precise and rapid dye method for measuring cell growth / cell kill.Journal of immunological methods 119, 203-210. After treating the mixture of turmeric, Seokchangpo, and hot water extracts prepared in Example 1 to the PC12 cells cultured in 1 according to the concentration (0.00625, 0.0125, 0.025, 0.05, 0.1%) according to the method of 24) After time, MTT (3- (4,5-dimethyl-2-yl) -2,5-diphenyl tetra zolium bromide) assay was performed to determine the toxicity of the hydrothermal extract mixture of the present invention to PC12 cells against the cells. .
The results are shown in Fig.
As shown in Figure 5, turmeric, Seokchangpo, hot water extract of the raw paper of the present invention was confirmed that there is no toxic effect when compared to the control at a concentration of 0.1% or less.
< Experimental Example 6> curcuma , Seokchangpo , Paper Of hot-water extract Mixture of Hydroxyl On the radical by DNA Antioxidant effect
In order to determine the effect of inhibiting the DNA oxidation by the hydroxyl radical of the turmeric, Seokchangpo, hot water extract of raw paper of the present invention, the following experiment was performed.
1. Extraction of Genomic DNA
Genomic DNA was extracted from PC12 cells, which are mouse melanoma cells, to determine the degree of oxidative damage caused by hydroxyl radicals generated by the Fenton reaction. That is, genomic DNA was extracted from PC12 cells following a slightly modified DNA standardization procedure (see Sambrook, J., Fritsch, E. and Maniatis, T. 1989 Molecular cloning, Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY). .
2. Genomic DNA Oxidation Reaction
DNA oxidation exposed to hydroxyl radicals generated by the Fenton reaction was performed according to previously tested methods (Milne, L., Nicotera, P., Orrenius, S. and Burkitt, M. 1993. Effects of glutathione and chelating agents on copper-mediated DNA oxidation: pro-oxidant and antioxidant properties of glutathione.Archives of biochemistry and biophysics 304, 102-102. First, a mixture of turmeric, spearmint, and hot water extract prepared in Example 1 of test concentration (0.00625, 0.0125, 0.025, 0.05, 0.1%) in 100 μl DNA solution, 200 μM, 1 mM and 50 μg / ml genome DNA was added. The reaction mixture was allowed to react at room temperature for 30 minutes and then the reaction was terminated by addition of 10 mM EDTA.
3. Measurement of electrophoresis and genomic DNA oxidation inhibitory effect
20 μl of the reaction mixture containing 1 μg genomic DNA was electrophoresed at 100 V on a 1% agarose gel for 30 minutes. The gel was stained with 1 mg / ml ethidium bromide and washed with water, and then observed using a LAS3000 image analyzer (Fujifilm Life Science, Tokyo, Japan).
The results are shown in Fig.
As shown in FIG. 6, when DNA is exposed to hydroxyl radicals generated by the Fenton reaction, DNA was degraded by oxidation. On the other hand, the group treated with a mixture of turmeric, stone Changpo, raw paper hot water extract prepared in Example 1 of the present invention, the DNA band becomes clear as the concentration increases, turmeric, stone Changpo, hot water extract of raw paper of the present invention It was confirmed that the mixture significantly inhibited oxidative damage of genomic DNA by hydroxyl radicals (***, P <0.001).
< Experimental Example 7> curcuma , Seokchangpo , Paper Of hot-water extract ACE of the mixture ( Angiotensin I converting Enzyme ) Inhibitory effect
In order to measure the antihypertensive activity of the mixture of turmeric, Seokchangpo, and hot water extracts of the present invention, the effect of inhibiting the activity of angiotensin converting enzyme (ACE) was measured (ACE assay). Specifically, ACE activity is 1 g / 10 mL of acetone powder (Sigma, USA) prepared from rabbit lung tissue in 0.1 M sodium borate buffer (pH 8.3) containing 0.3 M NaCl. After extraction at 4 ° C. for 2 hours at a concentration of (w / v), centrifugation (4 ° C., 4,000 rpm, 40 minutes) was carried out to obtain an ACE coenzyme solution. ACE inhibitory activity was 0.1 M boric acid in the test concentrations (0.00625, 0.0125, 0.025, 0.05, 0.1%) of turmeric, Seokchangpo, hot water extracts of raw paper and positive control (0.01% Captopril), respectively. 100 μL of sodium buffer (pH 8.3) and 50 μL of ACE coenzyme solution were added, followed by preliminary reaction at 37 ° C. for 5 minutes, followed by HHL in 5 mL of 0.1 M sodium borate buffer (pH 8.3) containing 0.3 M NaCl. 50 μL of substrate prepared by adding 25 mg of hippuryl-histidyl-leucine) was added and reacted at 37 ° C. for 30 minutes. 250 μL of 1 N HCl was added to stop the reaction. Then, 1.5 mL of ethyl acetate was added thereto, stirred for 15 seconds, and centrifuged (3,000 rpm, 5 min, 4 ° C.) to obtain 1 mL of the supernatant. . The supernatant was completely dried and 3 mL of distilled water was added to the mixture, followed by stirring. The absorbance was measured at 228 nm with a spectrophotometer. 50 μL of distilled water was used as a negative control group, and Captopril 0.01% was used as a positive control group to compare the activity of the mixture of turmeric, Seokchangpo, and hot water extracts of raw paper. For ACE enzyme activity, the absorbance ratio before and after sample addition was converted into% value.
The results are shown in Fig.
As shown in Figure 7, the experimental group treated with turmeric, stone Changpo, raw paper hot water extract each showed a lower inhibitory effect compared to the positive control, whereas the turmeric, stone Changpo, raw paper hot water extract mixture of the present invention concentration of 0.05% or more It was confirmed that the ACE activity inhibitory effect was significantly superior to the control at (*, P <0.05).
< Experimental Example 8> curcuma , Seokchangpo , Paper Of hot-water extract Mixture of AchE ( Acetylcholine esterase) inhibitory activity
In order to confirm the AChE activity inhibitory effect of the mixture of turmeric, Seokchangpo, and raw hot water extract of the present invention, the following experiment was performed.
50 μL of AchE (25 mU / mL) isolated from PC12 cells was used as a reaction enzyme, and 1 mM acetylcholine iodide dissolved in 0.1 M sodium phosphate buffer (pH 8.0) was used as a substrate. Prepared by dissolving, 5-dithio-bis (2-nitrobenzoic acid) and 15 mg sodium hydrogen carbonate in 10 ml of 0.1 M sodium phosphate buffer (pH 7.0).
The enzyme reaction was developed as follows. 20 μl of a mixture of turmeric, stone spear, and hot water extract prepared in Example 1 (produced to have a concentration of 1 mg / ml in the final reaction solution) in 2 ml of 0.1 M sodium phosphate buffer (pH 8.0), 200 After adding 10 μl of 10 mM DTNB solution and 100 μl of enzyme (0.03 U) and maintaining the mixture at 37 ° C. for 10 minutes, 200 μl of the substrate was added and reacted for 3 minutes, and then the absorbance value was measured at 412 nm with a spectrophotometer. The activity of AchE converted the absorbance ratio before and after sample addition into% value. As a positive control, rivastigmine 0.01% was used.
The results are shown in Fig.
As shown in Figure 8, the experimental group treated with turmeric, stone Changpo, raw paper extract each showed a lower AChE activity inhibitory effect than the control group, whereas the turmeric, stone Changpo, raw paper hot water extract mixture of the present invention is 0.05% or more It was confirmed that the AchE activity inhibitory effect was significantly better than the control at the concentration (*, P <0.05).
Examples of formulations for the composition of the present invention are illustrated below.
< Formulation example 1> Preparation of Pharmaceutical Formulations
1. Manufacturing of powder
2 g of a mixture of turmeric, stone Changpo, and raw hydrothermal extract
1g lactose
The above components were mixed and packed in airtight bags to prepare powders.
2. Preparation of tablets
100mg of turmeric, Seokchangpo, and raw hydrothermal extract
Corn Starch 100mg
Lactose 100mg
2 mg magnesium stearate
After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.
3. Preparation of Capsule
100mg of turmeric, Seokchangpo, and raw hydrothermal extract
Corn Starch 100mg
Lactose 100mg
2 mg magnesium stearate
After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.
< Formulation example 2> Manufacturing of food
Food containing a mixture of turmeric, Seokchangpo, raw hot water extract of the present invention was prepared as follows.
1. Preparation of cooking seasoning
A health seasoning cooking seasoning was prepared as a mixture of turmeric, Seokchangpo, and hot water extract of 20-95% by weight.
2. Manufacture of tomato ketchup and sauce
Health promotion tomato ketchup or sauce was prepared by adding 0.2-1.0 wt% of the mixture of turmeric, Seokchang-po and raw hydrothermal extract to tomato ketchup or sauce.
3. Manufacture of flour food
0.5 to 5.0% by weight of a mixture of turmeric, Seokchangpo, and raw hot water extract was added to the flour, and bread, cake, cookies, crackers, and noodles were prepared using the mixture to prepare health-promoting foods.
4. Manufacture of soups and gravies
0.1-5.0% by weight of a mixture of turmeric, Seokchang-po, and raw hot water extract was added to soups and broths to prepare meat products for health promotion, soups and noodles for noodles.
5. Manufacture of ground beef
Health promotion ground beef was prepared by adding 10% by weight of a mixture of turmeric, Seokchangpo, and raw hot water extracts to ground beef.
6. Manufacture of dairy products
5-10% by weight of the mixture of turmeric, Seokchang-po, and raw hydrothermal extract was added to the milk, and various dairy products such as butter and ice cream were prepared using the milk.
< Formulation example 3> Manufacturing of beverage
1. Manufacture of carbonated beverages
5-10% of sugar, 0.05-0.3% citric acid, 0.005-0.02% caramel, 0.1-1% of vitamin C are mixed, and 79-94% purified water is mixed to make syrup, and the syrup is 85-98 Sterilizing at 20 ℃ for 180 seconds to mix with a cooling water 1: 1 ratio and then injected 0.5 ~ 0.82% of carbon dioxide gas to prepare a carbonated beverage comprising a mixture of turmeric, quarry, raw paper hot water extract of the present invention.
2. Manufacture of health drinks
Instant sterilization by homogeneously mixing a mixture of subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and turmeric, quarry, and hot water extract After this, it was packaged in small packaging containers such as glass bottles and plastic bottles to prepare a healthy beverage.
3. Preparation of Vegetable Juice
5 g of the mixture of turmeric, Seokchangpo, and raw hot water extract was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice for health promotion.
4. Preparation of Fruit Juice
Health mixture fruit juice was prepared by adding 1 g of a mixture of turmeric, Seokchangpo, and raw hot water extract to 1,000 ml of apple or grape juice.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020120019268A KR20130097537A (en) | 2012-02-24 | 2012-02-24 | Composition comprising mixture of hot water extract of curcuma longa radix, acorus gramineus soland and polygala tenuifolia to enhance cognition and memory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020120019268A KR20130097537A (en) | 2012-02-24 | 2012-02-24 | Composition comprising mixture of hot water extract of curcuma longa radix, acorus gramineus soland and polygala tenuifolia to enhance cognition and memory |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20130097537A true KR20130097537A (en) | 2013-09-03 |
Family
ID=49449879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020120019268A KR20130097537A (en) | 2012-02-24 | 2012-02-24 | Composition comprising mixture of hot water extract of curcuma longa radix, acorus gramineus soland and polygala tenuifolia to enhance cognition and memory |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR20130097537A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104224992A (en) * | 2014-08-01 | 2014-12-24 | 张宁 | Dietetic nursing formula for preventing and treating alzheimer disease and applications of dietetic nursing formula |
| WO2016137040A1 (en) * | 2015-02-27 | 2016-09-01 | 부산대학교 산학협력단 | Composition for preventing or treating cerebrophathy or neurological disorder, containing medicinal plant extract as active ingredient |
| KR20180005806A (en) * | 2016-07-07 | 2018-01-17 | 우석대학교 산학협력단 | Composition for improvement of memory and cognition ability, prevention, delay, treatment or improvement of Alzheimer's disease, comprising extracts of Ecklonia stolonifera, Curcuma longa Rhizoma, Zingiber officinale Roscoe and red ginseng |
| KR20190001164A (en) * | 2017-06-26 | 2019-01-04 | 우석대학교 산학협력단 | Composition for improvement of memory and cognition ability, prevention, delay, treatment or improvement of Alzheimer's disease, comprising extracts of Curcuma longa Rhizoma, Chaenomeles sinensis Koehne fruit and Zingiber officinale Roscoe rhizome |
| KR102217607B1 (en) * | 2020-06-01 | 2021-02-18 | 국립낙동강생물자원관 | A composition for improving memory and cognitive function, preventing and improving ischemia reperfusion injury including acorus gramineus soland extract |
-
2012
- 2012-02-24 KR KR1020120019268A patent/KR20130097537A/en not_active Application Discontinuation
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104224992A (en) * | 2014-08-01 | 2014-12-24 | 张宁 | Dietetic nursing formula for preventing and treating alzheimer disease and applications of dietetic nursing formula |
| WO2016137040A1 (en) * | 2015-02-27 | 2016-09-01 | 부산대학교 산학협력단 | Composition for preventing or treating cerebrophathy or neurological disorder, containing medicinal plant extract as active ingredient |
| KR20180005806A (en) * | 2016-07-07 | 2018-01-17 | 우석대학교 산학협력단 | Composition for improvement of memory and cognition ability, prevention, delay, treatment or improvement of Alzheimer's disease, comprising extracts of Ecklonia stolonifera, Curcuma longa Rhizoma, Zingiber officinale Roscoe and red ginseng |
| KR20190001164A (en) * | 2017-06-26 | 2019-01-04 | 우석대학교 산학협력단 | Composition for improvement of memory and cognition ability, prevention, delay, treatment or improvement of Alzheimer's disease, comprising extracts of Curcuma longa Rhizoma, Chaenomeles sinensis Koehne fruit and Zingiber officinale Roscoe rhizome |
| KR102217607B1 (en) * | 2020-06-01 | 2021-02-18 | 국립낙동강생물자원관 | A composition for improving memory and cognitive function, preventing and improving ischemia reperfusion injury including acorus gramineus soland extract |
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