KR101487742B1 - Antioxidative food composition comprising Shiitake Mushroom and Gastrodia elata Blume - Google Patents

Abstract

The present invention relates to a food or pharmaceutical composition having an antioxidant activity, which comprises a shiitake mushroom and a chum salmon extract as main components. The food or pharmaceutical composition according to the present invention has excellent free radical scavenging ability and inhibition of active oxygen generation, and is excellent in antioxidative effect and can further promote the therapeutic effect according to the compounding ratio.

Description

An antioxidative food composition comprising Shiitake mushroom and Gastrodia elata Blume,

The present invention relates to a food or pharmaceutical composition having an antioxidant activity, which comprises a shiitake mushroom and a chum salmon extract as main components.

Free oxygen radicals are toxic substances produced by oxygen-using cells, which damage cell membrane lipids and proteins and promote cell senescence. At this time, active oxygen among the generated oxides is known to be toxic to the human body and to be toxic by SOD which is an inhibitor of superoxide at the same time. The mechanism of the generation of reactive oxygen species is increased by the damage of mitochondrial function, cell membrane lipids, nucleic acid and enzymes involved in various metabolism, inflammation mechanism, etc. [Gollnick, PD, Berticci, LA, Kelso, TB, Witt, EH, Hodgson, DR The effect of high intensity exercise on the respiratory capacity of the skeletal muscle. Fluegers Arch, 415; 407-413, 1990.].

In the reduction of active oxygen electrons to water, superoxide radicals are generated in the electron transport system, and the unsaturated fatty acids in the lipid peroxidation state are reacted very sensitively to the free radical reaction by the active oxygen, (Chuang, C., Chiueh, < RTI ID = 0.0 > Iron overload, oxidative stress, and axonal dystrophy in brain disorders. Pediatric Neurology, 25 (2); 138-147, 2001.].

There are various non-enzymatic antioxidants such as SOD, CAT, and GPx and reduced glutathione (GSH) and uric acid in the body because of the mechanism of protecting against damage by active oxygen. SOD and nitric acid (NO) Highly reactive oxygen compounds such as free radicals and H ₂ O ₂ continue to physiologically occur during aerobic metabolism, destabilizing compounds because they have a strong ability to draw electrons from various organic and inorganic compounds. Of these reactive oxygen species, small amounts play an important role in physiological processes, and others are inactivated by enzymatic or nonenzymatic antioxidant defense mechanisms in cells. When these reactive oxygen species are excessively produced or when the antioxidant defenses of cells are damaged, they cause a chain reaction with proteins, lipids, nucleic acids, etc. in the cells to cause problems in the cell function, thereby causing DNA mutations, changes in structure and function of proteins, . Since reactive oxygen species are highly reactive and have a short half-life, they are measured using an indirect method to measure their activity.

SOD is superoxide radical (O ₂₎ an act of converting a H ₂ O ₂ and O _2, at which time the resulting H ₂ O ₂ are are removed by the CAT and GPx, CAT is H ₂ O from the H ₂ O ₂ And O ₂ to protect cells from the action of O ₂ . GPx oxidizes GSH to oxidized glutathione (GSSG) and removes H ₂ O ₂ . Since oxidized GSSG is reduced to GSH by consuming nicotinamide adenine dinucleotide phosphate (NADPH) by glutathione reductase, total glutathione is balanced in two forms: reduced form (GSH) and oxidized form (GSSG) . GPx and glutathione reductase regulate various types of peroxides such as H ₂ O ₂ and liquid hydrogen peroxide by glutathione. In the case of intracellular toxic or oxidative damage, the GSSG is gradually increased, leading to an imbalance of GSH / GSSG, and thus its role as a pre-defense is lost. Therefore, GSH is considered to be an important substance that inhibits cell damage by oxygen free radicals and plays an important role in intracellular detoxification with SOD (Kalra, SP, Sahu A., Kalra PS Agein of the neuropeptidergic signals in rats. J. Reprod. Fertil. Suppl., 46; 11-9, 1993.].

Mushrooms are higher fungi that form fruiting bodies to taxonomically form spores, belonging to bacillus or acacia. Mushrooms contain nutrients such as carbohydrates, proteins, minerals and vitamins, as well as unique flavors and textures, and have been used for a long time as edible and medicinal.

Among them, Shiitake mushroom (SM, Lentinus edodes ) is an edible mushroom belonging to the genus Bacillus sp. Shiitake mushrooms have a unique flavor and taste and are highly palatable natural materials. They exhibit antioxidant activity and blood coagulation inhibitory effect in terms of health functional properties. Shiitake mushrooms have the highest content of vitamin C in mushrooms and contain guanosine 5'-monophosphate (5'-GMP) and lenthionine as flavor and aroma components, and also contain various amino acids and ergosterol. Among the components, lenthionine has antitumor activity and antioxidant activity. [Reference: Choi, MY, Jung, TY, Hahm, KJ) Cytotoxic effects of hot water soluble polysaccharides from mushroom, lentinus edodes and vitamin A & E Supplementation against P ₃₈₈ cells. Korean J Nutrition. 28 (11): 1091-1099, 1995.].

On the other hand, it refers to the root of the Pegasus (天麻, Gastrodia elata) is a cock (Gastrodia elata Blume) can self-harm the perennial plant belonging to the Orchidaceae (Orchidaceae). Because there is no chlorophyll in Chunma, photosynthesis of nutrients by using carbon assimilation is impossible, and it grows by supplying nutrition with convenient mingling with mulberry mulberry ( Amillaria mellea ) because there is no root. The main treatments of chima are used for the treatment of seizures, tetanus, tidal glaucoma, dizziness, headache, nervous breakdown, etc .; pharmacological actions include sedation, anticonvulsant, analgesic, antiinflammation, increased heart and cerebral blood flow, , Increased antioxidant activity, and immunological activation. The active ingredient may be selected from the group consisting of gastrodin, vanillyl alcohol, glycosides, mucus, vitamin A, sitoslerol, vanillin, coumarin, beta-sitoslerol ), Benzaldehyde, acetylagstrodin, p-hydroxybenzyl alcohol, phenolic glycoside, organic acid, sugar, calcium, magnesium, iron, phosphorus, potassium and the like, It turned out. Studies on the components of the rhizome have shown that phenolic antioxidants (flavonoids, catechins, phenolic acids, etc.) among the constituents reduce lipid peroxides and reduce damage caused by active oxygen species such as superoxide. In addition to being effective in the treatment of tension, hypertension, diabetes, headache, and nervous breakdown, Chunma has various pharmacological components, and in one study, ρ-hydroxybenzyl alcohol and gastrodine, In the study.

Since the antioxidant activity of Chunma has been mainly used in the private sector for the treatment of epilepsy, it has various pharmacological components and it is effective for the treatment of tension, hypertension, diabetes, headache and nervous breakdown as well as antithrombotic action, (γ-amino-butyric acid) neurotransmitter regulating action has been revealed [Reference: Luo, H., Wang, L., Chen, H., Xue, Z. Effects of tian-ma injection on myocardial ischemia and lipid peroxidation in rabbits. Hwueh Pao, 23; 53-56, 1992.].

In relation to antioxidant activity, the activity of glutathione S-transferase, which is known to be an antioxidant system necessary for maintaining homeostasis from the active oxygen species present in vivo, [References] Tae Soo Kang, Youngjun Kwon, Hyejeong Kwon, Byunggon Choi, Jung Ji Hong, Park Yong Gil. A Study on the Component Analysis and In Vitro Bioactivity of Chumama Extract. The Korean journal of mycology, 30 (2); 136-141, 2002.]. However, systematic studies on the protective effect and mechanism of oxidative damage caused by antioxidative action of gastronidin are lacking.

In Patent Application Laid-Open No. 10-2013-0026123 (Mar. 23, 2013), a fermented fermented milk product produced according to the method disclosed in JP-A No. 10-2013-0026123 (2013.03.13.) Is used as a raw material for clinical and health-aid functional products requiring clinical efficacy of anti- A method of using the same is disclosed.

1. Published Patent Application No. 10-2013-0026123 (Mar. 13, 2013)

The inventors of the present invention have intensively studied to develop a product for providing a beneficial function to a human body, and as a result, it has been found that a food or pharmaceutical composition having antioxidant activity, which comprises mushroom and Chumma extract as main components, The present invention can be effectively used as a health functional food or pharmaceutical composition requiring such an antioxidative effect, and has completed the present invention.

Accordingly, it is an object of the present invention to provide a food or pharmaceutical composition having antioxidative activity, which comprises, as a main component, a mushroom and a chewing gum extract.

The food or pharmaceutical composition according to the present invention has excellent free radical scavenging ability and inhibition of active oxygen generation, and is excellent in antioxidative effect and can further promote the therapeutic effect according to the compounding ratio.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the results of measurement of erasing efficacy against DPPH radicals of a mixture of an extract of Angelica keiskei koidz.
FIG. 2 is a graph showing antioxidant efficacy of a mixture of chrysanthemum and altitude.

The present invention provides, in one aspect, a food or pharmaceutical composition having antioxidant activity, which comprises shiitake mushroom and Chumama extract as a major component.

The shiitake mushroom and Chimpanzee extract are prepared by mixing the dried shiitake and chewing gum in an appropriate ratio of 1: 0.5, preferably 1: 0.5;

To 3 to 6 times of 70 to 90% ethanol, preferably 80% ethanol, and extracting in a water bath at 95 to 100 ° C for 10 to 14 hours, preferably 12 hours, using a reflux condenser;

Cooling the extract to 45 to 55 캜, preferably about 50 캜;

Filtering the extract with multiple layers of gauze (e.g., 3 to 5 layers) to take the supernatant;

Repeating the extraction and filtration steps 2 to 5 times, preferably 3 times;

Collecting the filtrate, and concentrating the filtrate under reduced pressure using a rotary evaporator; And

Dissolving the concentrate in a small amount of distilled water (preferably, 1/5 to 1/20 of the volume of the concentrate), lyophilizing the extract solution at -90 ° C to -70 ° C to obtain an extract in powder form Lt; / RTI >

The pharmaceutical compositions of the present invention can be particularly advantageously used for diseases requiring blood circulation improvement and antioxidative efficacy, and those skilled in the art can easily identify individuals who are presumed to suffer from such diseases, conditions and disorders.

The pharmaceutical composition of the present invention can be formulated in combination with a known pharmaceutical carrier. In general, the active ingredient is mixed with a pharmaceutically acceptable liquid or solid carrier and, if necessary, a solvent, a dispersant, an emulsifier, a buffer, a stabilizer, an excipient, a binder, a disintegrant, , Powders such as powders, powders and capsules, liquid solutions such as liquid solutions, suspensions, and emulsions. It may also be a dried product which can be made into a liquid phase by the addition of a suitable carrier before use.

The pharmaceutical composition of the present invention may be administered by any of the parenteral preparations such as oral agents, injections, and spot-applying agents.

The pharmaceutical carrier can be selected in accordance with the above dosage form and formulation, and in the case of an oral preparation, for example, starch, lactose, white sugar, mannitol, carboxymethyl cellulose, corn starch, inorganic salt and the like are used. In the preparation of an oral preparation, a binder, a disintegrant, a surfactant, a lubricant, a flowability promoter, a mating agent, a colorant, a perfume, and the like may be further blended.

On the other hand, in the case of the parenteral preparation, the composition containing the protease, which is the active ingredient of the present invention, is dissolved in distilled water for injection, physiological saline, aqueous glucose solution, vegetable oil for injection, sesame oil, soybean oil, Propylene glycol, polyethylene glycol and the like, and if necessary, adding a bactericide, stabilizer, isotonizing agent, an anhydrous agent and the like.

The pharmaceutical composition of the present invention is administered by an appropriate route according to the form of the preparation. The method of administration is not particularly limited, and it can be administered by the contents, external application and injection. The injection can be administered, for example, intravenously, intramuscularly, subcutaneously, intradermally, and the like.

The dose of the pharmaceutical composition of the present invention is appropriately set in accordance with the form of the pharmaceutical composition, the administration method, the purpose of use, and the age, body weight, and symptoms of the patient to which the active ingredient is administered. The amount is, for example, 10 μg to 10 mg / kg per adult day. However, those skilled in the art will appreciate that the pharmacokinetic properties of a particular reagent and its mode and route of administration; Age, health, weight; It will be understood that the dosage varies depending on known factors such as the nature and extent of the symptoms, the type of concurrent treatment, the frequency of treatment and the desired effect.

In addition, the composition of the present invention comprising shiitake mushroom and Chimpanzee extract may be provided as a health functional food as an antioxidant.

The formulations of the food composition according to the present invention can be formulated in the form of powders, granules, tablets, or beverages (e.g., oral preparations, pouches, or drinks) according to conventional methods in the art .

The dosage of the food composition of the present invention is appropriately set in accordance with the form of the composition, the method of administration, the purpose of use, and the age, weight and symptom of the individual to which the composition is applied. It is 20mg ~ 100mg per 450mg of 1 capsule and it can be dose of 6 capsules per adult basis. Of course, since the dosage varies depending on various conditions, the amount may be less than the above range, or may be more than the above range.

The food composition according to the present invention may contain conventional additives or additives, or sweeteners such as licorice, bean curd powder, wolfberry extract, St. John's Wort, selenium yeast, vitamin C, citric acid, nicotinic acid, One or more ingredients selected from the group consisting of sodium, aspartame, saccharin, pectin, maltitol, sorbitol, xylitol, guar gum, skim milk powder and oligosaccharide may be added to increase taste and aesthetics. They are suitably used at about 20 to 80% based on the total weight of the composition of the present invention.

Hereinafter, the composition for antioxidation of shiitake mushroom and chewing gum according to the present invention will be described by way of preferred embodiments. It is to be understood that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention, It will be obvious.

Example 1. Preparation of Chimma single extract (GS-1)

One kilogram of dried chestnut (purchased from a herbal medicine cooperative in Hwasun, Jeonnam) was cut into pieces and added to 5,000 ml of 80% ethanol. After reflux condenser was installed, it was extracted for 12 hours in a water bath at 95 ~ 100 ℃. The extract was cooled to about 50 캜 and filtered with several layers of gauze to take the supernatant. The extraction and filtration operations were repeated three times, and the supernatants were combined and concentrated under reduced pressure using a rotary evaporator. The resulting extract solution was lyophilized at -80 ° C to obtain 217 g of a powdered chimma single extract (GS-1), which was used as a sample of a subsequent experimental example.

Example 2: Preparation of high altitude single extract (GS-2)

(GS-2) (155.8 g) in powder form was obtained as a sample of the subsequent experimental example, except that the dried chestnut was replaced with a shiitake mushroom .

Example 3: Preparation of chondroma and altitude mixed extract (GS-3)

185.8 g of powdery mixed extract (GS-3) was obtained by carrying out the same reaction step as in Example 1 except that 1 kg of dried chestnut was mixed with 0.5 kg of chymosome and 0.5 kg of oyster, Respectively.

Example 4: Preparation of chimma and an altitude mixed extract (GS-4)

195.4 g of powdery mixed extract (GS-4) was obtained by carrying out the same reaction step as in Example 1, except that 1 kg of dried chestnut was mixed with 0.67 kg of chrysanthemum and 0.33 kg of altitude. Respectively.

Example 5: Preparation of chrysanthemum and altitude mixed extract (GS-5)

176.9 g of a powdery mixed extract (GS-5) was obtained by carrying out the same reaction step as in Example 1 except that 1 kg of dried chestnut was mixed with 0.33 kg of chrysanthemum and 0.67 kg of an altitude, Respectively.

Example 6: Pharmaceutical formulation

Form of formulation (tablets)

Mixed extract of altimeter and horse chestnut (Example 4) 10 mg

Lactose 80 mg

Starch 17 mg

Magnesium stearate 3 mg

Crystalline cellulose 10 mg

One embodiment of the present invention is the use of the antioxidant as an example of an effective ingredient of the tablets shown above. Tablets may be prepared by conventional methods and one preferred embodiment is in the form of tablets or soft capsules with conventional enteric coatings (e. G., Hydroxypropylmethylcellulose phthalate), sugar coating or coatings.

Example 7: Functional food preparation

100 g of the shiitake mushroom and the chewing gum extract obtained in Example 4 were granulated using a granulator, and vacuum packed in a usual manner to prepare granules.

Example 8: Preparation of beverage

20 g of citric acid and 17 g of aspartame were added to 100 g of the shiitake mushroom and the chewing gum extract obtained in Example 5, and the remaining was added with purified water to prepare 10 liters of drinking water and pasteurized.

Experimental Example 1: Measurement of free radical scavenging activity

In order to measure the free radical scavenging effect of the chimma and the altitude mixed extract of Examples 1 to 5, the following method was used as described in the previous study (Blois, M.S., 1199-1200, 1958).

16 mg of DPPH (1,1-diphenyl-2-picrylhydrazyl, 0.2 mM) was dissolved in 100 ml of ethanol, and 100 ml of distilled water was mixed and filtered through a filter paper. To 2.5 ml of this filtrate, And 0.5 ml of altitude mixed extracts were mixed and reacted at room temperature for 10 minutes. To measure the amount of DPPH remaining, the change in absorbance at 528 nm was measured using a microplate reader (Molecular Device, Sunnyvale, Calif., USA). The ascorbic acid component was used as a positive control. The results of measurement of the eradication efficacy of DPPH radicals of the mixture of Angelica keiskei koidz. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the results of measurement of the eradication efficacy against DPPH radicals of a mixture of chymus and altitude. FIG.

As can be seen from the experimental results shown in FIG. 1, it was confirmed that GS-4 had excellent free radical scavenging activity.

Experimental Example 2: Determination of antioxidant enzyme activities by mixed extracts of Chunma and altitude

Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) are three enzymes important for cell defense against ROS-mediated cellular damage. Antioxidant defense systems in the body, including SOD, CAT and GPx, can be damaged by the aging process. The resulting damage can even lead to mutations in genetic material that can lead to disruption of biological processes and death of cells and the development of cancer. Thus, the effects of oxidative stress and the progression of degenerative diseases and aging can be prevented.

In order to investigate the antioxidative effect of the extracts and the mixed extracts, the superoxide dismutase (SOD) activity was measured by a method of inhibiting autoxidation from epinephrine to adrenochromine, (CAT) activity was measured by the method of Karmazyn et al. (Karmazyn et al., H424-431, 1990), which measures the amount of oxygen generated upon decomposition of H ₂ O ₂ by this enzyme with an oxygen monitor. Glutathione peroxidase (GPx) activity was measured using the method of Paglia and Valentine (Paglia and Valentine, 158-169, 1967) and the results are shown in FIG. 2 is a graph showing the antioxidant activity of the mixture of Angelica keiskei koidz.

As shown in FIG. 2, it was confirmed that GS-4 increased oxidase activity and decreased the rate of active oxygen generation.

As described above, the mixed extract of the chimpanzee and the starch of the present invention exhibits free radical scavenging ability and antioxidant enzyme activity, and in particular, 80% or more of the Gastro elata : Shiitake mushroom is mixed at a weight ratio of 0.67: 0.33, It was confirmed that the mixed extract (GS-4) available in ethanol has excellent free radical scavenging ability and antioxidant enzyme activity and can be effectively used as an antioxidant functional material containing an active ingredient for anti-aging and antioxidant effect, The therapeutic effect can be promoted depending on the blending ratio.

Having described specific portions of the present invention in detail, those skilled in the art will appreciate that these specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereto will be. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (3)

Including shiitake mushroom and Chumama extract as main ingredients,
The shiitake mushrooms and Chimpanzee extracts were prepared by finely dividing the dried shiitake and chestnuts and mixing them at a weight ratio of 1: 0.5;
Adding the mixture obtained in the above step to 3- to 6-fold 70 to 90% ethanol, and extracting the mixture using a reflux condenser in a water bath at 95 to 100 ° C for 10 to 14 hours;
Cooling the extract obtained in the above step to 45 to 55 ° C;
Filtering the extract which has been cooled in the above step with multiple layers of gauze to take a supernatant;
Repeating the extraction and filtration steps 2 to 5 times;
Collecting the resulting filtrate, and concentrating the filtrate under reduced pressure using a rotary evaporator; And
Dissolving the concentrate obtained in the above step in distilled water and lyophilizing the extract solution at -90 ° C to -70 ° C to obtain an extract in powder form. delete The food composition of claim 1, wherein the composition is in the form of a pouch, a drink, a powder, a granule, or a tablet.

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