CN114716282A - Preparation method of mushroom residue carbon-based microbial organic fertilizer - Google Patents
Preparation method of mushroom residue carbon-based microbial organic fertilizer Download PDFInfo
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- CN114716282A CN114716282A CN202210613197.0A CN202210613197A CN114716282A CN 114716282 A CN114716282 A CN 114716282A CN 202210613197 A CN202210613197 A CN 202210613197A CN 114716282 A CN114716282 A CN 114716282A
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 82
- 230000000813 microbial effect Effects 0.000 title claims abstract description 67
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 53
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 47
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 39
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 32
- 238000002156 mixing Methods 0.000 claims abstract description 27
- 238000001035 drying Methods 0.000 claims abstract description 22
- 235000013379 molasses Nutrition 0.000 claims abstract description 17
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 16
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 16
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 14
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000203 mixture Substances 0.000 claims abstract description 12
- 239000002105 nanoparticle Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 10
- 229910052814 silicon oxide Inorganic materials 0.000 claims abstract description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 7
- 230000003213 activating effect Effects 0.000 claims abstract description 6
- 230000004913 activation Effects 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 6
- 238000009630 liquid culture Methods 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 241000193755 Bacillus cereus Species 0.000 claims description 10
- 241000194107 Bacillus megaterium Species 0.000 claims description 10
- 244000063299 Bacillus subtilis Species 0.000 claims description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- 239000000377 silicon dioxide Substances 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 2
- 238000012545 processing Methods 0.000 abstract description 3
- 230000009920 chelation Effects 0.000 abstract description 2
- 238000009826 distribution Methods 0.000 abstract description 2
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 description 14
- 238000013461 design Methods 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 239000003337 fertilizer Substances 0.000 description 5
- 241000235342 Saccharomycetes Species 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002686 phosphate fertilizer Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/40—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a preparation method of a carbon-based microbial organic fertilizer from mushroom residues, which comprises the following steps: drying mushroom residues, crushing, uniformly mixing with a sodium bicarbonate solution, standing for 30-40 min, filtering, and taking mushroom residues; uniformly mixing and stirring the obtained mushroom residues and a proper amount of mesoporous silicon oxide nanoparticles, and pyrolyzing the mixture for 0.5 to 1 hour at 500 to 550 ℃ under the protection of nitrogen to obtain mushroom residue carbon groups; inoculating the compound microbial agent into a liquid culture medium for culture and activation, and activating at 25-30 ℃ to form a microbial agent; respectively taking a proper amount of molasses, the microbial agent and the mushroom residue carbon base, mixing the molasses, the microbial agent and the mushroom residue carbon base according to a proportion, metering the volume to 1L by using clear water, and standing the mixture at 30 ℃ for 24 hours to form the mushroom residue carbon-based microbial organic fertilizer. The mushroom residue carbon base obtained by the invention has the characteristics of developed aperture, uniform aperture distribution, large specific surface area and strong adsorption and chelation capacity, and provides a new method for processing edible mushroom residue.
Description
Technical Field
The invention relates to the field of fertilizer preparation, and particularly relates to a preparation method of a carbon-based microbial organic fertilizer containing mushroom residues.
Background
China is a large edible fungus producing country, and in recent years, with the development of edible fungus production, the edible fungus residue yield of China is huge. For a large amount of edible fungus dregs, if the edible fungus dregs are not properly treated, the environment is polluted, and resources are wasted.
At present, the treatment method of the edible fungus residues mainly comprises the steps of discarding, burning and processing the edible fungus residues into feed or fertilizer, wherein the fungus residues contain rich protein and other nutrient components, the random discarding is a waste of resources, and meanwhile, the breeding of mould and pests is easy to cause environmental pollution; the incineration can only obtain about 10 percent of energy, which is unreasonable utilization of biomass; the use of the mushroom dregs as raw materials for preparing the feed is safe, but the effects of the mushroom dregs are not completely accepted and accepted by consumers, so the market has certain limitation; the fungus dregs need longer time for composting. Therefore, further research on the fungus residues is needed, and new ideas and new fields are continuously developed for the development of the edible fungus industry.
Disclosure of Invention
In order to solve the problems, the invention provides a preparation method of a carbon-based microbial organic fertilizer containing mushroom residues.
In order to achieve the purpose, the invention adopts the technical scheme that:
a preparation method of a mushroom residue carbon-based microbial organic fertilizer comprises the following steps:
s1, drying mushroom residues, crushing, uniformly mixing with a sodium bicarbonate solution, standing for 30-40 min, filtering, and taking mushroom residues;
s2, mixing and stirring the obtained mushroom residues and a proper amount of mesoporous silicon oxide nanoparticles uniformly, and pyrolyzing the mixture for 0.5-1 h at 500-550 ℃ under the protection of nitrogen to obtain mushroom residue carbon groups;
s3, inoculating the compound microbial agent in a liquid culture medium for culture and activation, and activating for 48-72 hours at 25-30 ℃ to form the microbial agent;
s4, respectively taking a proper amount of molasses, the microbial agent and the mushroom residue carbon base, mixing the molasses, the microbial agent and the mushroom residue carbon base according to a proportion, metering the volume to 1L by using clear water, and standing the mixture at 30 ℃ for 24 hours to form the mushroom residue carbon-based microbial organic fertilizer.
In a further design of the scheme, in the step S1, the mushroom dregs are placed in a drying furnace, the mushroom dregs are dried at 50-55 ℃ until the water content is about 10%, fresh air is continuously introduced into the drying furnace during the drying period, and the flow rate of the fresh air is 3500-4600 Nm3And h, then crushing to about 100 meshes.
In a further design of this embodiment, in step S1, the ratio of the mushroom dregs to the sodium bicarbonate solution is 1g: 20-30 mL, and the concentration of the sodium bicarbonate solution is 5% -10%.
According to a further design of the scheme, in the step S2, the mixing ratio of the mushroom dregs to a proper amount of mesoporous silica nanoparticles is 10: 1-1.5.
As a further design of the present solution, in step S3, the liquid medium is an MRS liquid medium.
In a further design of this embodiment, in step S3, the composite microbial agent is obtained by mixing Bacillus subtilis, Bacillus cereus, saccharomycetes and Bacillus megaterium.
In a further embodiment of the present invention, in step S3, the 1L microbial agent contains Bacillus subtilis 109cfu～2×10 9cfu, Bacillus megaterium 109cfu～2×10 9cfu, Bacillus cereus 109cfu～2×10 9cfu, yeast 109cfu～2×10 9cfu。
As a further design of the scheme, in the step S4, each liter of the mushroom residue carbon-based microbial organic fertilizer contains 20-30 mL of microbial agent, 500-600 mg of mushroom residue carbon base and 5-10 mg of molasses.
The invention has the following beneficial effects:
1) the obtained mushroom residue carbon base has the characteristics of developed aperture, uniform aperture distribution, large specific surface area and strong adsorption and chelation capacity, can adsorb nutrient substances in the apertures to realize slow release, is favorable for continuous absorption and utilization of crops, and provides a new method for processing edible mushroom residues.
2) The obtained mushroom residue carbon-based microbial organic fertilizer is rich in a large amount of trace nutrient elements necessary for plants, and can stimulate the physiological activity of each nutrient element in soil, so that each nutrient element does not generate antagonistic action in the soil, thereby promoting the absorption and utilization of each nutrient element by crops and increasing the crop yield.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
A preparation method of a mushroom residue carbon-based microbial organic fertilizer comprises the following steps:
s1, placing mushroom residues in a drying furnace, drying at 50 ℃ until the water content is about 10%, continuously introducing fresh air into the drying furnace during drying, wherein the flow rate of the fresh air is 4000Nm3Pulverizing to about 100 mesh, mixing with sodium bicarbonate solution, standing for 35min, filtering, and collecting residue; wherein the ratio of the mushroom dregs to the sodium bicarbonate solution is 1g to 20mL, and the concentration of the sodium bicarbonate solution is 10 percent;
s2, mixing and stirring the obtained mushroom residues and a proper amount of mesoporous silicon oxide nanoparticles uniformly, and pyrolyzing the mixture for 1h at 500 ℃ under the protection of nitrogen to obtain mushroom residue carbon groups; wherein the mixing ratio of the fungus residues to a proper amount of mesoporous silica nanoparticles is 10: 1;
s3, inoculating the compound microbial agent in an MRS liquid culture medium for culture and activation, and activating for 60 hours at 25 ℃ to form the microbial agent; wherein the compound microbial agent is prepared by mixing Bacillus subtilis, Bacillus cereus, saccharomycetes and Bacillus megaterium; 1L of microbial agent contains Bacillus subtilis 1.5 × 109cfu, Bacillus megaterium 1.5X 109cfu, Bacillus cereus 1.5X 109cfu, yeast 1.5 × 109cfu；
And S4, respectively taking a proper amount of molasses, the microbial agent and the mushroom residue carbon base, mixing the molasses, the microbial agent and the mushroom residue carbon base according to a proportion, fixing the volume to 1L by using clear water, and standing the mixture at 30 ℃ for 24 hours to form the mushroom residue carbon-based microbial organic fertilizer, wherein each liter of the mushroom residue carbon-based microbial organic fertilizer contains 20mL of the microbial agent, 500mg of the mushroom residue carbon base and 5 mg of the molasses.
Example 2
A preparation method of a mushroom residue carbon-based microbial organic fertilizer comprises the following steps:
s1, placing mushroom residues in a drying furnace, drying at 55 ℃ until the water content is about 10%, continuously introducing fresh air into the drying furnace during drying, wherein the flow rate of the fresh air is 4000Nm3Pulverizing to about 100 mesh, mixing with sodium bicarbonate solution, standing for 35min, filtering, and collecting residue; wherein the ratio of the mushroom dregs to the sodium bicarbonate solution is 1g to 30mL, and the concentration of the sodium bicarbonate solution is 5 percent;
s2, mixing and stirring the obtained mushroom residues and a proper amount of mesoporous silicon oxide nanoparticles uniformly, and pyrolyzing the mixture for 0.5 h at 550 ℃ under the protection of nitrogen to obtain mushroom residue carbon groups; wherein the mixing ratio of the fungus residues to a proper amount of mesoporous silicon oxide nano particles is 10: 1.5;
s3, inoculating the compound microbial agent in an MRS liquid culture medium for culture and activation, and activating for 60 hours at 25 ℃ to form the microbial agent; wherein the compound microbial agent is prepared from Bacillus subtilisMixing Bacillus subtilis, Bacillus cereus, yeast and Bacillus megaterium; 1L of microbial agent contains Bacillus subtilis 1.5 × 109cfu, Bacillus megaterium 1.5X 109cfu, Bacillus cereus 1.5X 109cfu, yeast 1.5 × 109cfu；
And S4, respectively taking a proper amount of molasses, the microbial agent and the mushroom residue carbon base, mixing the molasses, the microbial agent and the mushroom residue carbon base according to a proportion, fixing the volume to 1L by using clear water, and standing the mixture at 30 ℃ for 24 hours to form the mushroom residue carbon-based microbial organic fertilizer, wherein each liter of the mushroom residue carbon-based microbial organic fertilizer contains 30mL of the microbial agent, 600mg of the mushroom residue carbon base and 10 mg of the molasses.
Example 3
A preparation method of a mushroom residue carbon-based microbial organic fertilizer comprises the following steps:
s1, placing mushroom residues in a drying furnace, drying at 50 ℃ until the water content is about 10%, continuously introducing fresh air into the drying furnace during drying, wherein the flow rate of the fresh air is 4000Nm3Pulverizing to about 100 mesh, mixing with sodium bicarbonate solution, standing for 35min, filtering, and collecting residue; wherein the ratio of the mushroom dregs to the sodium bicarbonate solution is 1g to 25mL, and the concentration of the sodium bicarbonate solution is 7.5 percent;
s2, mixing and stirring the obtained mushroom residues and a proper amount of mesoporous silicon oxide nanoparticles uniformly, and pyrolyzing the mixture for 0.75 h at 520 ℃ under the protection of nitrogen to obtain mushroom residue carbon groups; wherein the mixing ratio of the fungus residues to a proper amount of mesoporous silicon oxide nano particles is 10: 1.25;
s3, inoculating the compound microbial agent in an MRS liquid culture medium for culture and activation, and activating for 60 hours at 25 ℃ to form the microbial agent; wherein the compound microbial agent is prepared by mixing Bacillus subtilis, Bacillus cereus, saccharomycetes and Bacillus megaterium; 1L of microbial agent contains Bacillus subtilis 109cfu～2×10 9cfu, Bacillus megaterium 109cfu～2×10 9cfu, Bacillus cereus 109cfu～2×10 9cfu, yeast 109cfu～2×10 9cfu；
And S4, respectively taking a proper amount of molasses, the microbial agent and the mushroom residue carbon base, mixing the molasses, the microbial agent and the mushroom residue carbon base according to a proportion, fixing the volume to 1L by using clear water, and standing the mixture at 30 ℃ for 24 hours to form the mushroom residue carbon-based microbial organic fertilizer, wherein each liter of the mushroom residue carbon-based microbial organic fertilizer contains 25mL of the microbial agent, 550mg of the mushroom residue carbon base and 7.5 mg of the molasses.
Compared with a conventional fertilization mode, the field test shows that 20% of fertilizer nitrogen and 30% of compound fertilizer are replaced by the mushroom residue carbon-based microorganism, the yield of wheat can be obviously improved, the yield of the wheat is increased by 10.3% -13.7%, and the economic benefit is obvious; meanwhile, the application amount of phosphate fertilizer and potash fertilizer can be reduced.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (8)
1. A preparation method of a carbon-based microbial organic fertilizer from mushroom residues is characterized by comprising the following steps:
s1, drying mushroom residues, crushing, uniformly mixing with a sodium bicarbonate solution, standing for 30-40 min, filtering, and taking mushroom residues;
s2, mixing and stirring the obtained mushroom residues and a proper amount of mesoporous silicon oxide nanoparticles uniformly, and pyrolyzing the mixture for 0.5-1 h at 500-550 ℃ under the protection of nitrogen to obtain mushroom residue carbon groups;
s3, inoculating the compound microbial agent in a liquid culture medium for culture and activation, and activating for 48-72 hours at 25-30 ℃ to form the microbial agent;
s4, respectively taking a proper amount of molasses, the microbial agent and the mushroom residue carbon base, mixing the molasses, the microbial agent and the mushroom residue carbon base according to a proportion, fixing the volume to 1L with clear water, and standing the mixture at 30 ℃ for 24 hours to form the mushroom residue carbon-based microbial organic fertilizer.
2. The method for preparing the carbon-based microbial organic fertilizer containing mushroom dregs as claimed in claim 1, wherein in step S1, the mushroom dregs are placed in a drying furnace, drying treatment is carried out at 50-55 ℃ until the water content is 10%, fresh air is continuously introduced into the drying furnace during drying, and the fresh air flow rate is 3500-4600 Nm3And/h, and then crushing to 100 meshes.
3. The method for preparing the mushroom residue carbon-based microbial organic fertilizer as claimed in claim 1, wherein in the step S1, the ratio of the mushroom residue to the sodium bicarbonate solution is 1 g/20-30 mL, and the concentration of the sodium bicarbonate solution is 5% -10%.
4. The method for preparing the mushroom residue carbon-based microbial organic fertilizer as claimed in claim 1, wherein in the step S2, the mixing ratio of the mushroom residue to a proper amount of mesoporous silica nanoparticles is 10: 1-1.5.
5. The method of claim 1, wherein in step S3, the liquid medium is MRS liquid medium.
6. The method of claim 1, wherein in step S3, the composite microbial agent is prepared by mixing Bacillus subtilis, Bacillus cereus, saccharomyces cerevisiae, and Bacillus megaterium.
7. The method for preparing the carbon-based microbial organic fertilizer containing mushroom dregs according to claim 1, wherein in the step S3, 1L of microbial agent contains bacillus subtilis 109cfu～2×10 9cfu, Bacillus megaterium 109cfu～2×10 9cfu, Bacillus cereus 109cfu～2×10 9cfu, yeast 109cfu～2×10 9cfu。
8. The method for preparing the mushroom residue carbon-based microbial organic fertilizer as claimed in claim 1, wherein in step S4, each liter of mushroom residue carbon-based microbial organic fertilizer contains 20-30 mL of microbial agent, 500-600 mg of mushroom residue carbon-based microbial organic fertilizer and 5-10 mg of molasses.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109134136A (en) * | 2018-08-01 | 2019-01-04 | 湖南工业大学 | A kind of biology carbon nitrogen fertilizer and preparation method thereof |
| CN109399633A (en) * | 2018-12-07 | 2019-03-01 | 重庆文理学院 | A kind of edible fungi residue charcoal and preparation method thereof |
| CN110964535A (en) * | 2019-11-28 | 2020-04-07 | 湖北同惠生物工程有限公司 | Soil remediation agent and preparation method and application thereof |
| CN112608199A (en) * | 2020-12-22 | 2021-04-06 | 李娜 | Method for preparing carbon-based organic fertilizer by using crop straws |
| CN112691640A (en) * | 2020-12-07 | 2021-04-23 | 天津大学 | Coprinus comatus mushroom dreg porous carbon adsorbent and preparation method and application thereof |
-
2022
- 2022-06-01 CN CN202210613197.0A patent/CN114716282A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109134136A (en) * | 2018-08-01 | 2019-01-04 | 湖南工业大学 | A kind of biology carbon nitrogen fertilizer and preparation method thereof |
| CN109399633A (en) * | 2018-12-07 | 2019-03-01 | 重庆文理学院 | A kind of edible fungi residue charcoal and preparation method thereof |
| CN110964535A (en) * | 2019-11-28 | 2020-04-07 | 湖北同惠生物工程有限公司 | Soil remediation agent and preparation method and application thereof |
| CN112691640A (en) * | 2020-12-07 | 2021-04-23 | 天津大学 | Coprinus comatus mushroom dreg porous carbon adsorbent and preparation method and application thereof |
| CN112608199A (en) * | 2020-12-22 | 2021-04-06 | 李娜 | Method for preparing carbon-based organic fertilizer by using crop straws |
Non-Patent Citations (1)
| Title |
|---|
| 鲜杨: "菌渣生物炭对镉污染土壤性质及小白菜产量、品质的影响", 《中国优秀硕士学位论文全文数据库（电子期刊），工程科技Ⅰ辑》 * |
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Application publication date: 20220708 |