CN102826907B - Nanometer concentrated enzyme organic fertilizer and preparation method thereof - Google Patents
Nanometer concentrated enzyme organic fertilizer and preparation method thereof Download PDFInfo
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- CN102826907B CN102826907B CN201210334020.3A CN201210334020A CN102826907B CN 102826907 B CN102826907 B CN 102826907B CN 201210334020 A CN201210334020 A CN 201210334020A CN 102826907 B CN102826907 B CN 102826907B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 239000003895 organic fertilizer Substances 0.000 title abstract description 16
- 239000003337 fertilizer Substances 0.000 claims abstract description 114
- 239000002689 soil Substances 0.000 claims abstract description 62
- 238000001035 drying Methods 0.000 claims description 33
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- 238000005453 pelletization Methods 0.000 claims description 23
- 150000001875 compounds Chemical class 0.000 claims description 22
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- 230000000694 effects Effects 0.000 abstract description 11
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- 239000000463 material Substances 0.000 abstract description 4
- 229910021392 nanocarbon Inorganic materials 0.000 abstract 2
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- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
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- 241000196324 Embryophyta Species 0.000 description 20
- 239000002086 nanomaterial Substances 0.000 description 18
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- 239000002105 nanoparticle Substances 0.000 description 15
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- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 13
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- 102000035195 Peptidases Human genes 0.000 description 10
- 229920000742 Cotton Polymers 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 230000035558 fertility Effects 0.000 description 6
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- 239000005416 organic matter Substances 0.000 description 5
- 229910052700 potassium Inorganic materials 0.000 description 5
- 239000011591 potassium Substances 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
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- 239000011574 phosphorus Substances 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
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- 239000002028 Biomass Substances 0.000 description 1
- RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 description 1
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- 108010042407 Endonucleases Proteins 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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Images
Abstract
The invention provides a nanometer concentrated enzyme organic fertilizer and a preparation method thereof. The nanometer concentrated enzyme organic fertilizer, based on mass percent, comprises 5%-10% of nanometer concentrated enzyme and 90%-95% of organic fertilizer, wherein, the nanometer concentrated enzyme, based on mass percent, comprises the following components: 2%-4% of nano-carbon, 1%-3% of nano-silicon, 20%-40% of protease, 30%-40% of ligninase, 20%-40% of soil enzyme and 1%-3% of bacillus subtilis. The small-scale nanometer materials with high surface activity, namely, nano-carbon and nano-silicon are added, and thus absorption ability of the plants on the fertilizer is intensified; utilization rate of the fertilizer is increased, and loss of organic fertilizer is decreased; moreover, by means of combining biologic enzyme with the nanometer materials, not only absorption for enzyme molecules and nanometer materials is guaranteed, but effective time of biologic enzyme in soil is increased, and use efficiency of organic fertilizer is raised.
Description
Technical field
The present invention relates to the organic fertilizer production field, particularly relate to concentrated enzyme fertilizer of a kind of nanometer and preparation method thereof.
Background technology
At present domestic manure type is a lot, the stalk of the residue material slag that main raw material is fermentation enterprise, methane slag, fermentation, animal excrement etc., fertilizer can improve edatope, slow down due to the soil compaction caused to fertilize for a long time, farm crop can not crop rotation etc. problem.China finds the reasons such as the effective constituent of farm manure is lower, and release rate is slow, and crop yield is low at the initial stage eighties, and the agri-scientific research worker organic composition that begins one's study is high, the organic fertilizer of long action time, and current domestic fertilizer market is comparatively ripe.
Domestic fertilizer of a great variety, but the utilization ratio of fertilizer is generally lower, is only 30% ~ 40%, lower utilization rate of fertilizer has caused the massive losses of fertilizer, and therefore, how improving utilization rate of fertilizer is problem demanding prompt solution in China's agricultural production process.
Summary of the invention
Based on this, be necessary to provide concentrated enzyme fertilizer of nanometer that a kind of utilization rate of fertilizer is higher and preparation method thereof.
A kind of nanometer concentrates the enzyme fertilizer, by mass percentage, comprises the fertilizer of the concentrated enzyme of 5% ~ 10% nanometer and 90% ~ 95%;
Wherein, described nanometer concentrates enzyme, by mass percentage, comprises following component:
Nano-sized carbon 2% ~ 4%;
Nano-silicon 1% ~ 3%;
Proteolytic enzyme 20% ~ 40%;
Lignoenzyme 30% ~ 40%;
Soil enzyme 20% ~ 40%; And
Subtilis 1% ~ 3%.
In embodiment, described fertilizer by mass percentage, comprises following component therein:
Plant ash 20% ~ 30%;
Biogas fermentation slag 10% ~ 30%;
Corn fermentation slag 20% ~ 30%;
Humic acids 20% ~ 30%; And
Wilkinite 5% ~ 10%.
In embodiment, described soil enzyme is grouped into by the one-tenth of following mass percent therein:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
In embodiment, the viable count of described subtilis is 2 * 10 therein
10cFU/g.
The preparation method of the concentrated enzyme fertilizer of a kind of nanometer comprises the following steps:
Preparation of nano concentrates enzyme, and described nanometer concentrates enzyme, by mass percentage, comprises following component:
Nano-sized carbon 2% ~ 4%;
Nano-silicon 1% ~ 3%;
Proteolytic enzyme 20% ~ 40%;
Lignoenzyme 30% ~ 40%;
Soil enzyme 20% ~ 40%; And
Subtilis 1% ~ 3%;
The preparation fertilizer;
By mass percentage, the concentrated enzyme of the described nanometer by 5% ~ 10% is mixed with 90% ~ 95% described fertilizer, obtains compound; And
Described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
In embodiment, described soil enzyme is grouped into by the one-tenth of following mass percent therein:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
In embodiment, described fertilizer by mass percentage, comprises following component therein:
Plant ash 20% ~ 30%;
Biogas fermentation slag 10% ~ 30%;
Corn fermentation slag 20% ~ 30%;
Humic acids 20% ~ 30%; And
Wilkinite 5% ~ 10%.
In embodiment, mixing time is 1h ~ 2h therein.
In embodiment, be 10min ~ 30min time of drying therein, and drying temperature is 50 ℃ ~ 65 ℃.
In embodiment, the particle diameter of extruding pelletization is 1mm ~ 3mm therein.
Above-mentioned nanometer concentrates the enzyme fertilizer, added nano material nano-sized carbon and the nano-silicon of small scale, high surface energy activity, and nano material can strengthen the absorption of plant to fertilizer, improves utilization rate of fertilizer, reduces the organic fertilizer loss; Simultaneously, utilize biological enzyme combining nano material, both can guarantee the absorption of enzyme molecule and nano material, increased again the action time of biological enzyme in soil, also improved the utilization ratio of organic fertilizer.
The accompanying drawing explanation
The preparation method's of the concentrated enzyme fertilizer of the nanometer that Fig. 1 is an embodiment schema.
Embodiment
For above-mentioned purpose of the present invention, feature and advantage can be become apparent more, below the specific embodiment of the present invention is described in detail.A lot of details have been set forth in the following description so that fully understand the present invention.But the present invention can implement much to be different from alternate manner described here, those skilled in the art can be in the situation that do similar improvement without prejudice to intension of the present invention, so the present invention is not subject to the restriction of following public concrete enforcement.
The nanometer of one embodiment concentrates the enzyme fertilizer, by mass percentage, comprises the fertilizer of the concentrated enzyme of 5% ~ 10% nanometer and 90% ~ 95%;
Wherein, described nanometer concentrates enzyme, by mass percentage, comprises following component:
Nano-sized carbon 2% ~ 4%;
Nano-silicon 1% ~ 3%;
Proteolytic enzyme 20% ~ 40%;
Lignoenzyme 30% ~ 40%;
Soil enzyme 20% ~ 40%; And
Subtilis 1% ~ 3%.
Nano-sized carbon can be the DK201 model nano-sized carbon of Deco island, Beijing gold Science and Technology Ltd., and nano-silicon can be the DK203 model nano-silicon of Deco island, Beijing gold Science and Technology Ltd..Nano-sized carbon and nano-silicon are the nano particles of small scale, high surface energy activity, can strengthen the absorption of plant to fertilizer, improve utilization rate of fertilizer, reduce fertilizer loss.
Proteolytic enzyme can accelerate the degraded of protein matter in soil, improves soil fertility, improves the transportcapacity of cotton to nutritive substance.
Lignoenzyme can help to break the anti-degraded barrier of biomass of plant, for the degraded of lignocellulose, has vital role.
Preferably, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
Sucrase can become sucrose hydrolysis D-Fructose and D-Glucose, claims again saccharase.Generally, in soil, invertase activity is higher, and fertility is higher, and its activity can characterize the conversion situation of organic carbon in soil indirectly.
The hydrogen peroxide endonuclease capable reduces hydrogen peroxide to soil and biological toxic action, and its activity has substantial connection with aerobic microbiological quantity, soil fertility, and it can mean the intensity of soil oxidising process.
Phosphoric acid esterase be a kind of can be by the dephosphorylized enzyme of corresponding substrate, by the hydrolysis phosphate monoester, the phosphate group on substrate molecule is removed, and is generated phosphate anion and hydroxyl freely.The soil organophosphorus transforms and restricted by the multiple factor, and the especially participation of Phosphoric acid esterase, can accelerate the dephosphorization speed of organophosphorus.
The viable count of described subtilis can be 2 * 10
10cFU/g.Subtilis can increase soil nutrient, improvement Soil structure, improve chemical fertilizer utilization ratio; Impel the organic matter decomposition in soil to become soil ulmin, stimulate plant growth; Certain fixed nitrogen, phosphorus decomposing, potassium decomposing effect are arranged.
Preferably, described fertilizer by mass percentage, comprises following component:
Plant ash 20% ~ 30%;
Biogas fermentation slag 10% ~ 30%;
Corn fermentation slag 20% ~ 30%;
Humic acids 20% ~ 30%; And
Wilkinite 5% ~ 10%.
Plant ash can sweetening of the soil acidity, the ground temperature that increases soil, control vegetable sprout term disease and strengthen crop disease-resistant and ability resistant to lodging.Plant ash, because containing 5% ~ 15% potassium element, can provide potassium element.
Containing the quick-acting nutrients such as a large amount of nitrogen, phosphorus, potassium in the biogas fermentation slag, can improve significantly Soil structure, physico-chemical property, enhancing soil fertility fertility, is the good fertilizer that quick-acting slow effects have both.
Soil can be improved and cultivate to the corn fermentation slag; The activating soil nutrient, balance nutrient is supplied with; Improve biological activity of soil, maintain species diversity, promote plant growth, improve the quality of crop product; Alleviate environmental pollution, save energy.
Humic acids can strengthen the fertility of soil and the structure of improvement soil.
Wilkinite has good adsorptivity, cohesiveness; Significantly improve anti-extrusion anti-caking effect; Nitrogen, phosphorus, potassium are had to the fractionation by adsorption dual regulation; Make fertilizer efficiency evenly extend fertilizer efficiency and improved the utilization ratio of the effective ingredient in fertilizer.
Above-mentioned nanometer concentrates the enzyme fertilizer, added nano material nano-sized carbon and the nano-silicon of small scale, high surface energy activity, and nano material can strengthen the absorption of plant to fertilizer, improves utilization rate of fertilizer, reduces the organic fertilizer loss; Simultaneously, utilize biological enzyme combining nano material, both can guarantee the absorption of enzyme molecule and nano material, increased again the action time of biological enzyme in soil, also improved the utilization ratio of organic fertilizer.
Refer to Fig. 1, the preparation method of the concentrated enzyme fertilizer of the nanometer of an embodiment comprises the following steps:
Step S10, the concentrated enzyme of preparation of nano, described nanometer concentrates enzyme, by mass percentage, comprises following component:
Nano-sized carbon 2% ~ 4%;
Nano-silicon 1% ~ 3%;
Proteolytic enzyme 20% ~ 40%;
Lignoenzyme 30% ~ 40%;
Soil enzyme 20% ~ 40%; And
Subtilis 1% ~ 3%.
Preferably, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
The viable count of described subtilis is 2 * 10
10cFU/g.
Step S20, preparation fertilizer.
Preferably, described fertilizer by mass percentage, comprises following component:
Plant ash 20% ~ 30%;
Biogas fermentation slag 10% ~ 30%;
Corn fermentation slag 20% ~ 30%;
Humic acids 20% ~ 30%; And
Wilkinite 5% ~ 10%.
Step S30, by mass percentage, the concentrated enzyme of the described nanometer by 5% ~ 10% is mixed with 90% ~ 95% described fertilizer, obtains compound.
Preferably, the concentrated enzyme of described nanometer and described fertilizer are poured in post-type mixer and mixed, obtain compound.Wherein, mixing time is 1h ~ 2h.
Step S40, described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
Preferably, described compound is carried out to extruding pelletization with disk extruder.The particle diameter of extruding pelletization is 1mm ~ 3mm.Be 10min ~ 30min time of drying, and drying temperature is 50 ℃ ~ 65 ℃.
The concentrated enzyme fertilizer of above-mentioned nanometer is during consumption is manured into soil as base fertilizer routinely, and the amount of application of every mu of concentrated enzyme fertilizer of ground nanometer is 30kg ~ 50kg.
The preparation method of the concentrated enzyme fertilizer of above-mentioned nanometer, introduce nano material, biological enzyme etc. in fertilizer, strengthens the absorption of plant to fertilizer, improves utilization rate of fertilizer, reduces the organic fertilizer loss; The concentrated enzyme of nanometer is first mixed to rear granulation with fertilizer, can make the concentrated enzyme of nanometer mix with fertilizer, be beneficial to the application of the concentrated enzyme fertilizer of nanometer.
Below in conjunction with specific embodiment, content of the present invention is further elaborated.The following examples are the concentrated enzyme amount of organic fertilizer of nanometer that apply in 0.07 hectare.
Embodiment 1
Preparation of nano concentrates enzyme, and described nanometer concentrates enzyme, by mass percentage, comprises following component:
Nano-sized carbon 2%;
Nano-silicon 1%;
Proteolytic enzyme 20%;
Lignoenzyme 36%;
Soil enzyme 40%; And
Subtilis 1%.
The preparation fertilizer, described fertilizer by mass percentage, comprises following component:
Plant ash 20%;
Biogas fermentation slag 20%;
Corn fermentation slag 20%;
Humic acids 30%; And
Wilkinite 10%.
By mass percentage, the concentrated enzyme of the described nanometer of 1.5kg is mixed with the described fertilizer of 28.5kg, obtained compound.
Described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
Wherein, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
The viable count of described subtilis is 2 * 10
10cFU/g.
Mixing time is 1h, and the particle diameter of extruding pelletization is 1mm, and be 15min time of drying, and drying temperature is 65 ℃.
Embodiment 2
Preparation of nano concentrates enzyme, and described nanometer concentrates enzyme, by mass percentage, comprises following component:
Nano-sized carbon 2%;
Nano-silicon 2%;
Proteolytic enzyme 40%;
Lignoenzyme 30%;
Soil enzyme 25%; And
Subtilis 1%.
The preparation fertilizer, described fertilizer by mass percentage, comprises following component:
Plant ash 30%;
Biogas fermentation slag 20%;
Corn fermentation slag 20%;
Humic acids 20%; And
Wilkinite 10%.
By mass percentage, the concentrated enzyme of the described nanometer of 2.1kg is mixed with the described fertilizer of 27.9kg, obtained compound.
Described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
Wherein, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
The viable count of described subtilis is 2 * 10
10cFU/g.
Mixing time is 2h, and the particle diameter of extruding pelletization is 3mm, and be 30min time of drying, and drying temperature is 65 ℃.
Embodiment 3
Preparation of nano concentrates enzyme, and described nanometer concentrates enzyme, by mass percentage, comprises following component:
Nano-sized carbon 4%;
Nano-silicon 3%;
Proteolytic enzyme 20%;
Lignoenzyme 40%;
Soil enzyme 30%; And
Subtilis 3%.
The preparation fertilizer, described fertilizer by mass percentage, comprises following component:
Plant ash 30%;
Biogas fermentation slag 25%;
Corn fermentation slag 20%;
Humic acids 20%; And
Wilkinite 5%.
By mass percentage, the concentrated enzyme of the described nanometer of 2.4kg is mixed with the described fertilizer of 27.6kg, obtained compound.
Described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
Wherein, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
The viable count of described subtilis is 2 * 10
10cFU/g.
Mixing time is 2h, and the particle diameter of extruding pelletization is 1mm, and be 10min time of drying, and drying temperature is 50 ℃.
Embodiment 4
Preparation of nano concentrates enzyme, and described nanometer concentrates enzyme, by mass percentage, comprises following component:
Nano-sized carbon 4%;
Nano-silicon 1%;
Protease 3 0%;
Lignoenzyme 40%;
Soil enzyme 22%; And
Subtilis 3%.
The preparation fertilizer, described fertilizer by mass percentage, comprises following component:
Plant ash 30%;
Biogas fermentation slag 10%;
Corn fermentation slag 20%;
Humic acids 30%; And
Wilkinite 10%.
By mass percentage, the concentrated enzyme of the described nanometer of 3kg is mixed with the described fertilizer of 27kg, obtained compound.
Described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
Wherein, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
The viable count of described subtilis is 2 * 10
10cFU/g.
Mixing time is 1.5h, and the particle diameter of extruding pelletization is 2mm, and be 20min time of drying, and drying temperature is 60 ℃.
Comparative Examples 1
The preparation fertilizer, described fertilizer by mass percentage, comprises following component:
Plant ash 20%;
Biogas fermentation slag 20%;
Corn fermentation slag 20%;
Humic acids 30%; And
Wilkinite 10%.
The fertilizer of 28.5kg is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
Wherein, the particle diameter of extruding pelletization is 1mm, and be 15min time of drying, and drying temperature is 65 ℃.
Comparative Examples 2
The concentrated enzyme of preparation, described concentrated enzyme, by mass percentage, comprise the proteolytic enzyme of following component: 0.3kg, the lignoenzyme of 0.54kg and the soil enzyme of 0.6kg.
The preparation fertilizer, described fertilizer by mass percentage, comprises following component:
Plant ash 20%;
Biogas fermentation slag 20%;
Corn fermentation slag 20%;
Humic acids 30%; And
Wilkinite 10%.
By mass percentage, described concentrated enzyme is mixed with the described fertilizer of 28.5kg, obtained compound.
Described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
Wherein, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
Mixing time is 1h, and the particle diameter of extruding pelletization is 1mm, and be 15min time of drying, and drying temperature is 65 ℃.
Comparative Examples 3
The preparation fertilizer, described fertilizer by mass percentage, comprises following component:
Plant ash 20%;
Biogas fermentation slag 20%;
Corn fermentation slag 20%;
Humic acids 30%; And
Wilkinite 10%.
By mass percentage, the subtilis of 0.015kg is mixed with the described fertilizer of 28.5kg, obtained compound.
Described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
Wherein, the viable count of described subtilis is 2 * 10
10cFU/g.
Mixing time is 1h, and the particle diameter of extruding pelletization is 1mm, and be 15min time of drying, and drying temperature is 65 ℃.
Comparative Examples 4
The concentrated enzyme of preparation, described concentrated enzyme, by mass percentage, comprise the proteolytic enzyme of following component: 0.3kg, the lignoenzyme of 0.54kg, the soil enzyme of 0.6kg and the subtilis of 0.015kg.
The preparation fertilizer, described fertilizer by mass percentage, comprises following component:
Plant ash 20%;
Biogas fermentation slag 20%;
Corn fermentation slag 20%;
Humic acids 30%; And
Wilkinite 10%.
By mass percentage, described concentrated enzyme is mixed with the described fertilizer of 28.5kg, obtained compound.
Described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer.
Wherein, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
The viable count of described subtilis is 2 * 10
10cFU/g.
Mixing time is 1h, and the particle diameter of extruding pelletization is 1mm, and be 15min time of drying, and drying temperature is 65 ℃.
Test-results
The soil of choosing without any processing is the blank group, and on the soil of blank group plant cotton; The fertilizer of the concentrated enzyme fertilizer of the nanometer of embodiment 1 ~ 4, Comparative Examples 1 ~ 4 is applied in the soil of 0.07 hectare as base fertilizer respectively, and on soil plant cotton.After after a while, the changing conditions of the active soil organic matter obtained and cotton test result are respectively as shown in table 1, table 2.
Table 1
As shown in Table 1, Comparative Examples 1 ~ 4 and embodiment 1 ~ 4 soil organism in the time of 150 days is significantly improved with respect to the soil organism of blank group, and the soil organism increase rate of embodiment 1 ~ 4, more than 200%, illustrates that the concentrated enzyme fertilizer of nanometer of embodiment 1 ~ 4 has improved the utilization ratio of fertilizer greatly.Simultaneously, the soil organism increase rate of embodiment 1, generally higher than Comparative Examples 1 ~ 4, illustrates adding of nano material, makes to form synergistic effect between nano material, enzyme and bacterium, and the concentrated enzyme fertilizer of this nanometer obviously improves the organic matter of soil, improves the utilization ratio of fertilizer.
Table 2
| The test sequence number | Dan Lingchong (g) | Ginning outturn (%) | Suede long (mm) | Per mu yield gined cotton (kg) | Stimulation ratio (%) |
| The blank group | 5.6 | 42.2 | 27.8 | 152.8 | - |
| Comparative Examples 1 | 5.8 | 42.5 | 27.8 | 155.0 | 1.44 |
| Comparative Examples 2 | 6.0 | 42.7 | 28.1 | 160.9 | 5.30 |
| Comparative Examples 3 | 5.8 | 42.7 | 28.0 | 160.7 | 5.17 |
| Comparative Examples 4 | 6.1 | 43.0 | 28.0 | 170.3 | 11.45 |
| Embodiment 1 | 6.1 | 43.5 | 28.2 | 179.5 | 17.47 |
| Embodiment 2 | 6.2 | 43.1 | 28.1 | 178.6 | 16.88 |
| Embodiment 3 | 6.2 | 43.2 | 28.2 | 179.2 | 17.28 |
| Embodiment 4 | 6.2 | 43.0 | 28.3 | 178.1 | 16.56 |
As shown in Table 2, the per mu yield lint yield that the per mu yield lint yield obtained with the fertilizer of Comparative Examples 1 ~ 4 and embodiment 1 ~ 4 obtains with respect to the blank group improves, and the per mu yield gined cotton stimulation ratio of embodiment 1 ~ 4 is more than 16%, illustrate that the concentrated enzyme fertilizer of nanometer of embodiment 1 ~ 4 has improved the organic matter of soil, has improved the output of per mu yield gined cotton simultaneously.In addition, the per mu yield gined cotton stimulation ratio that embodiment 1 obtains, generally higher than Comparative Examples 1 ~ 4, illustrates adding of nano material, makes to form synergistic effect between nano material, enzyme and bacterium, the concentrated enzyme fertilizer of this nanometer obviously improves the organic matter of soil, has also improved the output of per mu yield gined cotton.
Above-mentioned nanometer concentrates the enzyme fertilizer, added nano material nano-sized carbon and the nano-silicon of small scale, high surface energy activity, and nano material can strengthen the absorption of plant to fertilizer, improves utilization rate of fertilizer, reduces the organic fertilizer loss; Simultaneously, utilize biological enzyme combining nano material, both can guarantee the absorption of enzyme molecule and nano material, increased again the action time of biological enzyme in soil, also improved the utilization ratio of organic fertilizer; Form synergistic effect between nano material, enzyme and bacterium, the concentrated enzyme fertilizer of this nanometer obviously improves the organic matter of soil, has also improved the output of per mu yield gined cotton.
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (8)
1. the concentrated enzyme fertilizer of a nanometer, is characterized in that, by mass percentage, comprises 5%～10% the concentrated enzyme of nanometer and 90%～95% fertilizer;
Wherein, described nanometer concentrates enzyme, by mass percentage, comprises following component:
Described fertilizer by mass percentage, comprises following component:
2. the concentrated enzyme fertilizer of nanometer according to claim 1, is characterized in that, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
3. the concentrated enzyme fertilizer of nanometer according to claim 1, is characterized in that, the viable count of described subtilis is 2 * 10
10cFU/g.
4. the preparation method of the concentrated enzyme fertilizer of nanometer, is characterized in that, comprises the following steps:
Preparation of nano concentrates enzyme, and described nanometer concentrates enzyme, by mass percentage, comprises following component:
By mass percentage, the concentrated enzyme of the described nanometer by 5%～10% is mixed with 90%～95% described fertilizer, obtains compound; And
Described compound is carried out to extruding pelletization, after carry out drying, obtain the concentrated enzyme fertilizer of nanometer;
Wherein, described fertilizer by mass percentage, comprises following component:
5. the preparation method of the concentrated enzyme fertilizer of nanometer according to claim 4, is characterized in that, described soil enzyme is grouped into by the one-tenth of following mass percent:
Sucrase 45%;
Catalase-3 5%; And
Phosphoric acid esterase 20%.
6. the preparation method of the concentrated enzyme fertilizer of nanometer according to claim 4, is characterized in that, mixing time is 1h～2h.
7. the preparation method of the concentrated enzyme fertilizer of nanometer according to claim 4, is characterized in that, be 10min～30min time of drying, and drying temperature is 50 ℃～65 ℃.
8. the preparation method of the concentrated enzyme fertilizer of nanometer according to claim 4, is characterized in that, the particle diameter of extruding pelletization is 1mm～3mm.
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| CN103508622B (en) * | 2012-06-28 | 2015-02-04 | 国能电力集团有限公司 | Material and method for biologically purifying water body by using carbon nano-tube |
| CN103755478B (en) * | 2014-01-23 | 2016-03-09 | 贵州特力达纳米碳素科技有限公司 | A kind of Nano-carbon organic compound fertilizer |
| CN103772044B (en) * | 2014-01-23 | 2016-01-20 | 贵州特力达纳米碳素科技有限公司 | A kind of nano-sized carbon compoiste fertilizer |
| CN103772043B (en) * | 2014-01-23 | 2016-01-20 | 贵州特力达纳米碳素科技有限公司 | A kind of nano-sized carbon fertilizer |
| CN103755479B (en) * | 2014-01-23 | 2016-03-09 | 贵州特力达纳米碳素科技有限公司 | A kind of Nanocarbon organic nitrogen fertilizer |
| CN103755480B (en) * | 2014-01-23 | 2016-03-09 | 贵州特力达纳米碳素科技有限公司 | A kind of Nano carbon organic fertilizer |
| CN103755481B (en) * | 2014-01-23 | 2016-03-09 | 贵州特力达纳米碳素科技有限公司 | A kind of Nanocarbon organic fertilizer |
| CN104086284B (en) * | 2014-06-24 | 2016-06-08 | 山东光大肥业科技有限公司 | A kind of special organic foliar fertilizer of fruit type vegetable and its preparation method |
| CN104557279A (en) * | 2014-12-22 | 2015-04-29 | 郭爱民 | Seedling caring essence |
| CN104803790A (en) * | 2015-04-24 | 2015-07-29 | 苏州市新泾村农业基地专业合作社 | Endive biological enzyme organic compound fertilizer |
| CN104892071A (en) * | 2015-05-29 | 2015-09-09 | 苏州市新泾村农业基地专业合作社 | Bio-enzyme organic compound fertilizer for figs |
| CN105622296A (en) * | 2016-03-08 | 2016-06-01 | 韩志全 | Composite soil modifying agent and preparation method and application thereof |
| CN106083389A (en) * | 2016-06-28 | 2016-11-09 | 辽宁亩神农业科技有限公司 | Carbon enzyme silico-calcium bacterial manure |
| CN107568468B (en) * | 2017-10-12 | 2021-01-29 | 苏州昆蓝生物科技有限公司 | Micromolecular biological feed |
| CN107518153B (en) * | 2017-10-12 | 2021-01-29 | 苏州昆蓝生物科技有限公司 | Preparation method of micromolecular biological feed |
| CN112409062A (en) * | 2020-11-03 | 2021-02-26 | 康权 | Fertilizer additive, application thereof and chemical fertilizer |
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| CN101096329A (en) * | 2006-08-01 | 2008-01-02 | 左金煜 | Nanometer super composite fertilizer and method for preparing same |
| CN101362659B (en) * | 2007-08-10 | 2011-07-06 | 刘键 | Nano carbon long-acting environment friendly compound fertilizer |
| CN100462340C (en) * | 2007-11-23 | 2009-02-18 | 何章杰 | Method for manufacturing amino acid active fertilizer |
| CN102584434A (en) * | 2012-02-08 | 2012-07-18 | 陈书林 | Biological enzyme activated agricultural fertilizer |
| CN102628035B (en) * | 2012-04-16 | 2013-12-11 | 苏州昆蓝生物科技有限公司 | Compound enzyme preparation for tobacco planting |
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