CN114705878A - Multi-parameter full-automatic urine and saliva pretreatment and detection integrated instrument - Google Patents

Multi-parameter full-automatic urine and saliva pretreatment and detection integrated instrument Download PDF

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Publication number
CN114705878A
CN114705878A CN202210265613.2A CN202210265613A CN114705878A CN 114705878 A CN114705878 A CN 114705878A CN 202210265613 A CN202210265613 A CN 202210265613A CN 114705878 A CN114705878 A CN 114705878A
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liquid
pool
plate
peristaltic pump
sample
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CN202210265613.2A
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Inventor
王平
孙先佑
马驰宇
张砚驰
孔留兵
万浩
周书祺
王心怡
房瑞山
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Binjiang Research Institute Of Zhejiang University
Zhejiang University ZJU
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Binjiang Research Institute Of Zhejiang University
Zhejiang University ZJU
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Priority to CN202210265613.2A priority Critical patent/CN114705878A/en
Publication of CN114705878A publication Critical patent/CN114705878A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004—Cleaning sample transfer devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices
    • G01N35/1067—Multiple transfer devices for transfer to or from containers having different spacing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106—General arrangement of respective parts
    • G01N2021/0112—Apparatus in one mechanical, optical or electronic block
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N2021/3129—Determining multicomponents by multiwavelength light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1053—General features of the devices using the transfer device for another function for separating part of the liquid, e.g. filters, extraction phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers

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Abstract

The invention discloses a multi-parameter full-automatic pretreatment and detection integrated instrument for urine and saliva, which integrates the functions of filtering, extracting, washing a plate, automatically adding sample and detecting, can automatically finish the pretreatment of the urine and the saliva and the detection of markers such as oxalic acid, citric acid, Cyfra21-1 protein in the urine and has the function of automatic cleaning. The integrated instrument comprises a pretreatment module, a detection module and a control module. The detection module comprises two sets of lead screw modules, a dropper installation device, a spectrometer and a photodiode, and the control module comprises two lower computers and a plurality of drivers. The invention realizes the full-automatic design of pretreatment and detection of disease markers such as oxalic acid, citric acid, Cyfra21-1 protein in saliva and the like in urine, can be used for physical examination in hospitals, residential communities or families, and has high practicability.

Description

Multi-parameter full-automatic urine and saliva pretreatment and detection integrated instrument
Technical Field
The invention relates to a pretreatment and detection technology of human urine and saliva, in particular to an instrument capable of fully automatically completing pretreatment and detection integration of human urine and saliva.
Background
With the continuous acceleration of the life rhythm of people, the diet structure and the body health are not paid more and more attention, and the conditions of urinary calculus, oral cancer and the like are more and more. Timely hospitalization is one of the solutions, but most people are not aware of the severity of the disease at the early stages of its occurrence due to the lack of an instrument that can monitor the condition for a long period of time. Recent research shows that citric acid and oxalic acid in urine can be used as markers of urinary calculus, and Cyfra21-1 protein in saliva can be used as markers of oral cancer. Therefore, combining biochemical detection technology with automated technology would be expected to address this significant medical need.
Disclosure of Invention
The invention aims to provide a multi-parameter full-automatic urine and saliva pretreatment and detection integrated instrument aiming at the defects of the prior art.
The purpose of the invention is realized by the following technical scheme: a multi-parameter full-automatic urine and saliva pretreatment and detection integrated instrument comprises a case main body, a case top cover, a pretreatment module, a control module, a detection module and a liquid pool frame; the top cover of the case is arranged on the case main body; the top cover of the case is provided with a touch screen display component for man-machine interaction, and an upper computer is arranged in the touch screen display component; the pretreatment module, the control module and the detection module are arranged in the case main body, the control module is connected with the upper computer, and the pretreatment module and the detection module are connected with the control module;
the liquid pool frame is arranged on the outer side of the case main body and comprises eight liquid pool mounting grooves which are respectively provided with a pure water pool, a methanol pool, a dilute sulfuric acid pool, a diluent pool, an antibody solution pool, a developing solution pool, a plate washing liquid pool and a second waste liquid pool;
the pretreatment module comprises a multi-channel switching valve, a pretreatment peristaltic pump, a plate washing liquid adding peristaltic pump, a plate washing liquid sucking peristaltic pump, a waste liquid pool sucking peristaltic pump, a first micro plunger pump and a second micro plunger pump; the multi-channel switching valve is provided with eight liquid inlets and one liquid outlet, and the liquid inlets are respectively connected with a methanol pool, a pure water pool, a first sample pool, a dilute sulfuric acid pool, an antibody solution pool, a developing solution pool and air; the first sample pool is stored with urine or saliva, and different multi-channel switching valves are used for liquid inlets when detecting the urine or the saliva; the liquid outlet is connected to a liquid inlet of the pretreatment peristaltic pump through an extraction column; a liquid outlet of the pretreatment peristaltic pump is connected with a liquid inlet of a two-position three-way electromagnetic valve; the two-position three-way electromagnetic valve is provided with two liquid outlets which are respectively connected to the liquid inlets of the second waste liquid pool and the second sample pool; the liquid outlet of the second sample pool is connected with the liquid inlet of the first miniature plunger pump; a liquid outlet of the first miniature plunger pump is connected with a sample dropper in the detection module; the liquid inlet of the second miniature plunger pump is connected with the diluent pool, and the liquid outlet of the second miniature plunger pump is connected with the diluent drop pipe of the detection module; a liquid inlet of the plate washing liquid adding peristaltic pump is connected with the plate washing liquid pool, and a liquid outlet of the plate washing liquid adding peristaltic pump is connected with a plate washing liquid adding dropper of the detection module;
the detection module comprises a spectrometer, a photodiode, a dropper mounting device, a first waste liquid pool and a first lead screw module; the dropper mounting device comprises a dropper mounting support, a second lead screw module and a plate washing liquid imbibing dropper bracket; the dropper mounting support is provided with a plate washing liquid adding dropper, a movable through hole of the plate washing liquid absorbing dropper, a sample dropper and a diluent dropper; the second lead screw module is arranged on the dropper mounting support;
the plate washing liquid pipette support is arranged on the second lead screw module, a plate washing liquid pipette is arranged on the plate washing liquid pipette support, penetrates through the movable through hole of the plate washing liquid pipette and can move up and down under the driving of the second lead screw module and the limitation of the movable through hole of the plate washing liquid pipette; an ELISA plate bracket with an ELISA plate is arranged on the first lead screw module, and the first waste liquid pool is arranged below the four droppers; the plate washing liquid imbibition dropper is connected with a liquid inlet of the plate washing liquid imbibition peristaltic pump; a liquid outlet of the plate washing liquid suction peristaltic pump is connected to the second waste liquid pool; the first waste liquid pool is connected with a liquid inlet of a waste liquid pool liquid suction peristaltic pump, and a liquid outlet of the waste liquid pool liquid suction peristaltic pump is connected with the second waste liquid pool; the spectrometer is used for detecting the absorbance of oxalic acid in the urine sample in the ELISA plate and the absorbance of Cyfra21-1 protein in the saliva sample, and the photodiode is used for detecting the absorbance of citric acid in the urine sample in the ELISA plate;
the control module is used for receiving a control instruction of the upper computer, controlling the first lead screw module and the second lead screw module in the detection module to move and controlling the liquid inlet switching of a multi-channel switching valve in the pretreatment module, the liquid outlet switching of the two-position three-way electromagnetic valve, and the work of the pretreatment peristaltic pump, the plate washing liquid adding peristaltic pump, the plate washing liquid peristaltic pump, the waste liquid pool peristaltic pump, the first miniature plunger pump and the second miniature plunger pump; and simultaneously, receiving signal data of the spectrograph and the photodiode and feeding back the signal data to the upper computer.
Furthermore, the pretreatment module comprises a pretreatment module mounting plate connected in the case main body, and the pretreatment module mounting plate is connected with a first two-position two-way electromagnetic valve, a second two-position two-way electromagnetic valve, a third two-position two-way electromagnetic valve, a fourth two-position two-way electromagnetic valve, a two-position three-way electromagnetic valve, a first motor driver for driving the first screw module, a second motor driver for driving the second screw module, a third motor driver for driving the pretreatment peristaltic pump, a fourth motor driver for driving the plate washing liquid adding peristaltic pump, a fifth motor driver for driving the plate washing liquid imbibing peristaltic pump and a sixth motor driver for driving the waste liquid pool imbibing peristaltic pump, which are used for controlling the liquid path; the pretreatment module mounting plate is also provided with a peristaltic pump mounting plate, a plunger pump mounting plate, a switching valve mounting bracket and a first hydroelectric separation plate for separating a liquid path and a circuit; the peristaltic pump mounting plate is connected with a pretreatment peristaltic pump for sample pretreatment, a plate washing liquid adding peristaltic pump for dropwise adding a plate washing liquid to an ELISA plate, a plate washing liquid sucking peristaltic pump for sucking the plate washing liquid from the ELISA plate and a waste liquid tank sucking peristaltic pump for sucking a waste liquid from a first waste liquid tank; the plunger pump mounting plate is provided with a first micro plunger pump for adding a sample to the detection module and a second micro plunger pump for adding a diluent to the detection module; the switching valve mounting bracket is provided with a multi-channel switching valve; the first hydroelectric partition plate is provided with 4 oval through holes for circuit connection of the peristaltic pump and the motor drivers, and each motor driver is connected with the control module.
Furthermore, the control module is provided with a photodiode driver for driving a photodiode, a spectrometer driver for driving a spectrometer, a light source driver for driving an ultraviolet light source and a visible light source, and a first lower computer for transmitting instructions to the first motor driver, the second motor driver, the third motor driver, the fourth motor driver, the fifth motor driver, the sixth motor driver, the plunger pump driver, the multi-channel switching valve and the five-way relay module; the second lower computer is used for transmitting instructions to the photodiode driver, the spectrometer driver and the light source driver, and the switching power supply is used for supplying power to all electric equipment; the first lower computer and the second lower computer can be communicated with the upper computer in the touch screen display assembly.
Furthermore, the detection module further comprises a travel switch mounting support, an ultraviolet light source mounting support, a visible light source mounting support, a spectrometer mounting device and a photodiode mounting device;
the travel switch mounting support is provided with two waist-shaped holes and a travel switch for feeding back the position of the ELISA plate bracket, the ultraviolet light source mounting support is provided with a 340nm single-wavelength ultraviolet light source, and the visible light source mounting support is provided with a visible light source with the wavelength range of 390nm-700 nm; the ultraviolet light source and the visible light source are controlled by the control module to emit light;
the spectrometer mounting device comprises a spectrometer mounting support arranged on the detection module mounting plate, a spectrometer mounting groove is formed in the spectrometer mounting support, the spectrometer is mounted in the spectrometer mounting groove, a first light-transmitting hole is formed in the lower portion of the spectrometer mounting groove, spectrometer positioning threaded holes and spectrometer positioning screws for adjusting and fixing the position of the spectrometer are arranged on the left side and the right side of the spectrometer mounting groove, and the first light-transmitting hole and the visible light source are coaxial;
the photodiode mounting device comprises a photodiode mounting support and a photodiode gland, wherein the photodiode mounting support is provided with a photodiode mounting groove, the photodiode is mounted in the photodiode mounting groove, a second light-transmitting hole is formed in the photodiode mounting groove, and the second light-transmitting hole and the ultraviolet light source are coaxial; the photodiode mounting support is provided with a first left pressing screw hole and a first right pressing screw hole for fastening the photodiode; the center of the photodiode gland is provided with a waist-shaped hole, and two sides of the photodiode gland are provided with a second left pressing screw hole and a second right pressing screw hole for fastening the photodiode;
further, the bottom surface of the primary waste liquid tank is an inclined surface inclined towards the right rear vertex, and a waste liquid suction pipe is connected to the right rear vertex of the bottom surface of the primary waste liquid tank in an adhesive mode.
Furthermore, a filtering device is further installed on the outer side of the case main body, the filtering device comprises a filter installation cylinder, a first sample pool, a filter installation cover, a first-stage filter, a second-stage filter and a third-stage filter, a first vent hole for communicating with the atmosphere is formed in the side wall of the filter installation cylinder, a first sample pool liquid outlet is formed below the first sample pool, the first sample pool liquid outlet is connected with a multi-channel switching valve liquid inlet, the first sample pool is fixedly connected below the filter installation cylinder, and a filter partition plate with a circular hole in the center is arranged between the filter installation cylinder and the first sample pool; the filter comprises a primary filter, a secondary filter, a tertiary filter and a filter partition plate, wherein a 0.8um filter membrane is arranged in the primary filter, a 0.45um filter membrane is arranged in the secondary filter, a 0.22um filter membrane is arranged in the tertiary filter, an outlet of the primary filter is connected to an inlet of the secondary filter, an outlet of the secondary filter is connected to an inlet of the tertiary filter, the tertiary filter is arranged on the filter partition plate, and an outlet of the tertiary filter penetrates through a circular hole in the center of the filter partition plate; a circular through hole is formed in the center of the filter mounting cover, and an inlet of the primary filter penetrates through the circular through hole in the center of the filter mounting cover;
the lower part of the second sample cell is conical, a second sample cell liquid inlet and a second vent hole used for communicating atmosphere are arranged at the top of the second sample cell, and a second sample cell liquid outlet is arranged at the bottom of the second sample cell.
Furthermore, the pretreatment peristaltic pump, the plate washing liquid absorption peristaltic pump, the waste liquid pool liquid absorption peristaltic pump and the second waste liquid pool are connected through a liquid path four-way connector, and one-way valves are arranged on pipelines between the pretreatment peristaltic pump, the plate washing liquid absorption peristaltic pump, the waste liquid pool liquid absorption peristaltic pump and the liquid path four-way connector.
The multi-parameter full-automatic integrated instrument for pre-treating and detecting urine and saliva has the advantages that the multi-parameter full-automatic integrated instrument for pre-treating and detecting urine and saliva can fully automatically complete the processes of filtering, sample adding, plate washing, detecting and the like of a urine sample, has the functions of calibration and self-cleaning, is simple and convenient to operate, does not need operators to have professional medical backgrounds, and is wide in user range. Compared with a professional medical detection instrument, the medical detection instrument has the advantages of being light and portable, low in cost and the like, can be used in families or communities, and can provide long-term condition monitoring or early prevention for users. The invention is not only suitable for body fluid samples such as urine, saliva and the like, but also can be used for detection as long as the samples accord with the pretreatment and detection paradigm, and has larger application potential.
Drawings
FIG. 1 is a schematic view of a multi-parameter fully automatic integrated urine and saliva pretreatment and detection instrument of the present invention;
FIG. 2 is a schematic diagram of the main housing of the multi-parameter fully automatic integrated apparatus for urine and saliva pre-treatment and detection in FIG. 1;
FIG. 3 is an exploded view of a portion of the multi-parameter fully automated urine and saliva pretreatment and detection integrated instrument of FIG. 1;
FIG. 4 is a schematic diagram of a pre-processing module of the multi-parameter fully automatic urine and saliva pre-processing and detection integrated instrument of FIG. 3;
FIG. 5 is a schematic view of a pre-treatment module mounting plate of the multi-parameter fully automatic urine and saliva pre-treatment and detection integrated instrument of FIG. 4;
FIG. 6 is a schematic diagram of the liquid path four-way joint of the multi-parameter fully automatic integrated urine and saliva pretreatment and detection instrument of FIG. 4;
FIG. 7 is a schematic diagram of a control module of the multi-parameter fully automatic integrated urine and saliva pretreatment and detection instrument of FIG. 3;
FIG. 8 is a schematic view of a detection module of the multi-parameter fully automatic integrated urine and saliva pretreatment and detection instrument of FIG. 3;
FIG. 9 is an exploded view of a portion of the structure of the detection module of the multi-parameter fully automatic urine and saliva pretreatment and detection integrated instrument of FIG. 3;
FIG. 10 is a schematic view of the filter assembly of the multi-parameter fully automated integrated urine and saliva pretreatment and detection instrument of FIG. 1;
FIG. 11 is a schematic diagram of a second sample cell of the multi-parameter fully automated integrated urine and saliva pretreatment and detection instrument of FIG. 1;
FIG. 12 is a schematic diagram of the multi-channel switching valve of the multi-parameter fully automatic integrated urine and saliva pretreatment and detection instrument of FIG. 4;
the device comprises a case body 1, a case top cover 2, a pretreatment module 3, a detection module 4, a control module 5, a touch screen display component 20, a pretreatment module mounting plate 30, a first check valve 310, a second check valve 311, a third check valve 312, a liquid path four-way joint 32, a first two-position two-way solenoid valve 330, a second two-position two-way solenoid valve 331, a third two-position two-way solenoid valve 332, a fourth two-position two-way solenoid valve 333, a two-position three-way solenoid valve 34, a first motor driver 350, a second motor driver 351, a third motor driver 352, a fourth motor driver 353, a fifth motor driver 354, a sixth motor driver 355, a liquid path four-way joint first joint 320, a liquid path four-way joint second joint 321, a liquid path four-way joint third joint 322, a liquid path four-way joint fourth joint 323, a peristaltic pump mounting plate 301, a liquid path four-way joint mounting bracket 302 and a plunger pump mounting plate 303, a first hydroelectric partition plate 304, a pretreatment peristaltic pump 360, a wash plate liquid adding peristaltic pump 361, a wash plate liquid absorbing peristaltic pump 362, a waste liquid pool liquid absorbing peristaltic pump 363, a first micro plunger pump 370, a second micro plunger pump 371, a multi-channel switching valve 38, a module partition plate 19, a first dropper channel 191, a second dropper channel 192, a third dropper channel 193, a fourth dropper channel 194, a fifth dropper channel 195, a first circuit channel 190, a first rectangular boss group 196, a second rectangular boss group 300, a control module mounting plate 50, a third rectangular boss group 500, a plunger pump driver 51, a five-way relay module 52, a photodiode driver 56, a spectrometer driver 57, a light source driver 55, a first lower computer 53, a second lower computer 54, a switching power supply 58, a second circuit channel 501, a third circuit channel 502, a fourth circuit channel 503, a second hydroelectric partition plate 504, a detection module mounting plate 40, a travel switch mounting support 400, an ultraviolet light source mounting support 401, a visible light source mounting support 402, a spectrometer mounting device 41, a photodiode mounting device 42, a dropper mounting device 43, a first waste liquid tank 44, a first lead screw module 45, a first lead screw slide rail 450, a first lead screw slide mount 451, a first lead screw stepper motor 452, a travel switch 46, an ultraviolet light source 47, a visible light source 48, a spectrometer mounting support 410, a spectrometer mounting groove 411, a spectrometer 412, a first light transmission hole 413, a spectrometer positioning threaded hole 414, a spectrometer positioning screw 415, a photodiode mounting support 420, a photodiode gland 421, a photodiode mounting groove 422, a photodiode 423, a second light transmission hole 424, a first left compression screw hole 426, a first right compression screw hole 425, a second left compression screw hole 4211, a second right compression screw hole 4210, a dropper mounting support 430, a second lead screw module 431, a wash plate liquid pipette support 437, a wash plate liquid pipette 433, a wash plate liquid pipette movable through hole 434, a sample pipette 435, a diluent pipette 436, a second lead screw slide 4310, a second lead screw slide 4311, a second lead screw stepping motor 4312, a wash plate liquid pipette 438, an ELISA plate support 453, an ELISA plate 454, a waste liquid pipette 49, a power switch 59, a second sample cell support 10, a filter device support 11, a liquid cell support mounting boss 12, an extraction column support 13, a second sample cell first orifice 140, a second sample cell second orifice 141, a pure water cell orifice 180, a methanol cell orifice 181, a dilute sulfuric acid cell orifice 182, a diluent cell 183, an antibody solution cell orifice 184, a solution developing cell orifice 186, a wash plate liquid cell orifice, a second waste liquid cell orifice 187, an urine sample orifice 150, and a saliva sample orifice 151, an extraction column first pipe orifice 170, an extraction column second pipe orifice 171, a spare pipe orifice 188, a second sample cell 14, a filtering device 15, a liquid cell rack 16, an extraction column 17, a pure water cell 160, a methanol cell 161, a dilute sulfuric acid cell 162, a diluent cell 163, an antibody solution cell 164, a color solution cell 165, a plate washing liquid cell 166, a second waste liquid cell 167, a filter mounting cylinder 152, a first sample cell 154, a filter mounting cover 156, a primary filter 157, a secondary filter 158, a tertiary filter 159, a first vent hole 1521, a first sample cell liquid outlet 155, a filter partition 153, a first filtering device connecting table 1520, a second filtering device connecting table 1560, a second sample cell 142, a second vent hole 143, a second sample cell liquid outlet 144, a multi-channel switching valve liquid outlet 380, a first multi-channel switching valve 381, a second multi-channel switching valve liquid inlet 382, a third multi-channel switching valve liquid inlet 383, a fourth multi-channel switching valve liquid inlet 384, a fifth multi-channel switching valve liquid inlet 385, a sixth multi-channel switching valve liquid inlet 386, a seventh multi-channel switching valve liquid inlet 387, and an eighth multi-channel switching valve liquid inlet 388.
Detailed Description
The invention is described in detail below with reference to the figures and the embodiments.
As shown in fig. 1 to 11, the multi-parameter full-automatic integrated instrument for pretreatment and detection of urine and saliva comprises a case main body 1 and a case top cover 2 which are fixedly connected through trapezoidal bosses with 3 screw holes arranged at the top and the rear part. The case top cover 2 is provided with a touch screen display component 20 for man-machine interaction, the touch screen display component 20 is internally provided with an upper computer, instructions of a user can be transmitted to the first lower computer 53 and the second lower computer 54, and detection data of the first lower computer 53 and the second lower computer 54 are transmitted to the user. The case body 1 is provided with a pretreatment module 3, a detection module 4 and a control module 5.
The pretreatment module 3 comprises a pretreatment module mounting plate 30 connected with the machine case main body 1 through bolts, the pretreatment module mounting plate 30 is connected with a first check valve 310, a second check valve 311, a third check valve 312, a liquid path four-way joint 32, a first two-position two-way electromagnetic valve 330 and a second two-position two-way electromagnetic valve 331 used for liquid path control through bolts, a third two-position two-way solenoid valve 332, a fourth two-position two-way solenoid valve 333, a two-position three-way solenoid valve 34, a first motor driver 350 for driving a first lead screw stepping motor 452, a second motor driver 351 for driving a second lead screw stepping motor 4312, a third motor driver 352 for driving a pretreatment peristaltic pump 360, a fourth motor driver 353 for driving a plate washing liquid and liquid feeding peristaltic pump 361, a fifth motor driver 354 for driving a plate washing liquid and liquid suction peristaltic pump 362, and a sixth motor driver 355 for driving a waste liquid pool liquid suction peristaltic pump 363. The liquid path four-way joint 32 includes a liquid path four-way joint first joint 320, a liquid path four-way joint second joint 321, a liquid path four-way joint third joint 322, and a liquid path four-way joint fourth joint 323. The first check valve 310, the second check valve 311 and the third check valve 312 can ensure that the liquid flowing through the valve body can only flow from the liquid inlet to the liquid outlet. The pretreatment module mounting plate 30 is further provided with a peristaltic pump mounting plate 301, a plunger pump mounting plate 303, a switching valve mounting bracket 302 and a first hydroelectric separation plate 304 for separating a liquid path and a circuit. The peristaltic pump mounting plate 301 is connected with a pretreatment peristaltic pump 360 for sample pretreatment, a plate washing liquid adding peristaltic pump 361 for adding plate washing liquid dropwise to the elisa plate 454, a plate washing liquid sucking peristaltic pump 362 for sucking the plate washing liquid from the elisa plate 454, and a waste liquid tank sucking peristaltic pump 363 for sucking the waste liquid from the first waste liquid tank 44 through bolts. The plunger pump mounting plate 303 is provided with 4 threaded holes, and a first micro plunger pump 370 for adding a sample to a detection area and a second micro plunger pump 371 for adding a diluent to the detection area are mounted through bolts. The switching valve mounting bracket 302 is provided with 2 threaded holes, to which the multi-channel switching valve 38 is mounted by bolts. The first hydroelectric divider plate 304 is provided with 4 oval through holes for circuit connection of the peristaltic pump to the motor driver.
The case body 1 is provided with a module partition plate 19 for partitioning the pretreatment module 3 and the detection module 4, so that the pretreatment module 3 and the detection module 4 are isolated from water and electricity. The module partition plate 19 is provided with a first drop tube channel 191, a second drop tube channel 192, a third drop tube channel 193, a fourth drop tube channel 194, a fifth drop tube channel 195 for liquid path transmission, and a first circuit channel 190 for signal transmission between the detection module 4 and the pretreatment module 3 and the control module 5. The module partition plate 19 is further provided with 3 first rectangular boss groups 196 with circular screw holes in the center, the pretreatment module mounting plate 30 is provided with 3 support columns, and the top ends of the support columns are respectively provided with second rectangular boss groups 300 with circular screw holes in the center.
The control module 5 comprises a control module mounting plate 50, a third rectangular boss group 500 provided with screw holes of the control module mounting plate 50 is connected with the first rectangular boss group 196 through bolts, and three screw holes of the rear side of the control module mounting plate 50 are connected with the second rectangular boss group 300 through bolts so as to fix the control module 5. The control module mounting plate 50 is provided with a plurality of screw holes, and respectively provided with a plunger pump driver 51 for driving a first miniature plunger pump 370 and a second miniature plunger pump 371, a five-way relay module 52 for controlling a first two-position two-way solenoid valve 330, a second two-position two-way solenoid valve 331, a third two-position two-way solenoid valve 332, a fourth two-position two-way solenoid valve 333 and a two-position three-way solenoid valve 34, a photodiode driver 56 for driving a photodiode 423, a spectrometer driver 57 for driving a spectrometer 412, a light source driver 55 for driving a visible light source 48 and an ultraviolet light source 47, a first motor driver 350, a second motor driver 351, a third motor driver 352, a fourth motor driver 353, a fifth motor driver 354, a sixth motor driver 355, a plunger pump driver 51, a multi-channel switching valve 38, A first lower computer 53 for communicating commands to the five-way relay module 52, a second lower computer 54 for communicating commands to the photodiode driver 56, the spectrometer driver 57 and the light source driver 55, and a switching power supply 58 for powering all the consumers. The first lower computer 53 and the second lower computer 54 can independently communicate with the upper computer in the touch screen display assembly 20, so that stability of data transmission between the second lower computer 54 and the upper computer is guaranteed. The control module mounting board 50 is provided with a second circuit path 501, a third circuit path 502 and a fourth circuit path 503 for circuit connection between the pre-processing module 3 and the control module 5, which facilitates connection of different kinds of signal lines or power lines in a classified manner. The control module mounting plate 50 is also provided with a second hydro-electric separator plate 504 for separating the fluid path and the electrical circuit, which facilitates water-electricity isolation.
The detection module 4 comprises a detection module mounting plate 40 connected to the case body 1 through bolts, the detection module mounting plate 40 is provided with a travel switch mounting support 400, an ultraviolet light source mounting support 401, a visible light source mounting support 402, a spectrometer mounting device 41, a photodiode mounting device 42 and a dropper mounting device 43, and is connected with a first waste liquid pool 44 and a first lead screw module 45 through bolts.
The travel switch mounting support 400 is provided with two waist-shaped holes and is provided with a travel switch 46 for feeding back the position of the microplate holder 453, so that the closed-loop control of the position of the microplate holder 453 is realized conveniently. The ultraviolet light source mounting support 401 is provided with the ultraviolet light source 47 with single wavelength of 340nm, so that accurate detection of the citric acid in the urine is realized. The visible light source mounting support 402 is provided with the visible light source 48 with the wavelength range of 390nm-700nm, so that the detection of oxalic acid in urine and Cyfra21-1 protein in saliva is conveniently realized, and the detection of other markers is also conveniently expanded.
Spectrometer installation device 41 is including installing spectrometer installing support 410 on detection module mounting panel 40, is provided with spectrometer mounting groove 411 on spectrometer installing support 410, installs spectrum appearance 412 in spectrometer mounting groove 411, and opens the first light trap 413 with the same axle center with visible light source 48 below spectrometer mounting groove 411, is convenient for realize like this that the light path of visible light source 48 and spectrum appearance 412 aligns. Spectrometer positioning threaded holes 414 and spectrometer positioning screws 415 for adjusting and fixing the position of the spectrometer 412 are formed on the left and right sides of the spectrometer mounting groove 411, so that the position of the spectrometer 412 can be finely adjusted to obtain an optimal detection signal.
The photodiode mounting device 42 includes a photodiode mounting support 420 and a photodiode cover 421, the photodiode mounting support 420 is provided with a photodiode mounting groove 422, and a photodiode 423 is mounted in the photodiode mounting groove 422. A second light-transmitting hole 424 is formed in the photodiode mounting groove 422 coaxially with the ultraviolet light source 47, so that the light path alignment of the ultraviolet light source 47 and the photodiode 423 is facilitated. The photodiode mounting base 420 is opened with a first left pressing screw hole 426 and a first right pressing screw hole 425 for fastening the photodiode 423. The center of the photodiode gland 421 is provided with a waist-shaped hole for passing through a pin of the photodiode 423, two sides of the photodiode gland 421 are provided with a second left pressing screw hole 4211 and a second right pressing screw hole 4210, the first left pressing screw hole 426 and the second left pressing screw hole 4211 are connected through a bolt, and the first right pressing screw hole 425 and the second right pressing screw hole 4210 are connected through a bolt, so that the position of the photodiode 423 can be conveniently fastened, and the light path deviation is prevented.
The dropper mounting device 43 comprises a dropper mounting support 430 fixedly connected to the detection module mounting plate 40, a second lead screw module 431 and a wash plate liquid and suction dropper support 437, and a wash plate liquid and suction dropper 433, a wash plate liquid and suction dropper movable through hole 434, a sample dropper 435 and a diluent dropper 436 are arranged on the dropper mounting support 430. The distance between the axis of the wash plate liquid adding dropper 433 and the center of the movable through hole 434 of the wash plate liquid adding dropper, the distance between the center of the movable through hole 434 of the wash plate liquid adding dropper and the axis of the sample dropper 435, and the distance between the axis of the sample dropper 435 and the axis of the diluent dropper 436 are all 180 mm. The effective stroke of the second lead screw module 431 is 20mm, and comprises a second lead screw slide rail 4310, a second lead screw sliding table 4311 and a second lead screw stepping motor 4312 for driving the second lead screw sliding table 4311, wherein the second lead screw slide rail 4310 is connected to the dropper mounting support 430 through a bolt, the second lead screw sliding table 4311 is mounted on the second lead screw slide rail 4310, and the plate washing liquid suction dropper support 437 is mounted on the second lead screw sliding table 4311. The forward and backward rotation of the second lead screw stepping motor 4312 can drive the second lead screw sliding table 4311 to move up and down, so that the plate washing liquid pipette support 437 can be driven to move up and down. The plate washing liquid pipette rack 437 is provided with a plate washing liquid pipette 438, and the plate washing liquid pipette 438 is coaxial with the plate washing liquid pipette movable through hole 434, so that the plate washing liquid pipette 438 can move up and down through the plate washing liquid pipette movable through hole 434.
The effective stroke of the first lead screw module 45 is 200mm, and the first lead screw module 45 includes a first lead screw slide rail 450, a first lead screw sliding table 451 and a first lead screw stepping motor 452 for driving the first lead screw sliding table 451. First lead screw slide rail 450 passes through the bolt and installs in detection module mounting panel 40, installs first lead screw slip table 451 on first lead screw slide rail 450, installs ELIAS plate support 453 on the first lead screw slip table 451, has placed ELIAS plate 454 in the ELIAS plate support 453, and the positive and negative rotation of first lead screw step motor 452 can drive moving about first lead screw slip table 451, and then drives moving about ELIAS plate support 453 and ELIAS plate 454. The elisa plate 454 has 8 sample wells, and the center distance between each sample well and its adjacent sample well is 180mm, which is consistent with the distance between the axis of each dropper in the dropper mounting device 43 and the axis of its adjacent dropper, so as to facilitate the execution of the circulation operation. The ELIAS plate support 453 is internally provided with 8 round holes with the diameter of 1mm, which are respectively concentric with 8 sample cells of the ELIAS plate 454, thus being convenient for reducing the refraction and reflection of light and being convenient for the collimation of the light.
The bottom surface of the first waste liquid pool 44 is an inclined surface inclined towards the right rear vertex, and a waste liquid suction pipe 49 is glued at the right rear vertex of the bottom surface of the first waste liquid pool 44, so that waste liquid can be gathered towards the right rear vertex of the first waste liquid pool 44 under the action of gravity, and the waste liquid suction pipe 49 can suck the waste liquid clean to reduce the residue of the waste liquid.
A power switch 59 for controlling the connection and disconnection between an external power supply and the switching power supply 58 is installed on the left side of the casing main body 1. The right side of the case body 1 is provided with a second sample cell support 10, a filter device support 11, a liquid cell support mounting boss 12, an extraction column support 13, a first second sample cell orifice 140, a second sample cell orifice 141, a pure water cell orifice 180, a methanol cell orifice 181, a dilute sulfuric acid cell orifice 182, a diluent cell orifice 183, an antibody solution cell orifice 184, a chromogenic solution cell orifice 185, a plate washing liquid cell orifice 186, a second waste liquid cell orifice 187, a urine sample orifice 150, a saliva sample orifice 151, a first extraction column orifice 170, a second extraction column orifice 171 and a spare orifice 188. A second sample cell 14 is arranged in the second sample cell support 10, a filtering device 15 is arranged in the filtering device support 11, a liquid cell frame 16 is arranged on the liquid cell frame mounting boss 12 through a bolt, an extraction column 17 is arranged on the extraction column support 13, a liquid inlet is arranged at the top of the extraction column 17, and a liquid outlet is arranged at the bottom of the extraction column 17.
The liquid pool frame 16 is provided with 8 liquid pool mounting grooves, and is respectively provided with a pure water pool 160, a methanol pool 161, a dilute sulfuric acid pool 162, a diluent pool 163, an antibody solution pool 164, a color development solution pool 165, a plate washing liquid pool 166 and a second waste liquid pool 167. Pure water pond 160, methyl alcohol pond 161, dilute sulfuric acid pond 162, diluent pond 163, antibody solution pond 164, the drain pipe that is used for the atmospheric round hole of intercommunication and is used for going out liquid is all provided with at the top in coloration solution pond 165 and wash plate liquid pond 166, and the drain pipe all stretches the bottom in liquid pond, the top in second waste liquid pond 167 is provided with the feed liquor pipe that is used for the feed liquor and is used for communicateing atmospheric round hole, be convenient for like this guarantee each liquid pond internal gas pressure stable and be convenient for the high-speed joint of Teflon pipe and liquid pond.
The filtering apparatus 15 includes a filter mounting cylinder 152, a first sample tank 154, a filter mounting cap 156, a primary filter 157, a secondary filter 158, and a tertiary filter 159. The side wall of the filter mounting cylinder 152 is provided with a first vent hole 1521 for communicating with the atmosphere, a first sample cell liquid outlet 155 is arranged below the first sample cell 154, the first sample cell 154 is fixedly connected below the filter mounting cylinder 152, and a filter partition plate 153 with a circular hole at the center is arranged between the filter mounting cylinder 152 and the first sample cell 154. A0.8 um filter membrane is arranged in the primary filter 157, a 0.45um filter membrane is arranged in the secondary filter 158, a 0.22um filter membrane is arranged in the tertiary filter 159, an outlet of the primary filter 157 is connected with an inlet of the secondary filter 158, an outlet of the secondary filter 158 is connected with an inlet of the tertiary filter 159, the tertiary filter 159 is arranged on the filter partition 153, and an outlet of the tertiary filter 159 penetrates through a circular hole in the center of the filter partition 153, so that a sample can respectively pass through the primary filter 157, the secondary filter 158 and the tertiary filter 159 with the filter membranes gradually reduced, the situation that the tertiary filter 159 is blocked by larger impurities is avoided, and the filtered sample enters the first sample pool 154 to be stored. Two first filter device connecting stages 1520 having a screw hole opened at the center are provided at the upper portion of the filter mounting cylinder 152, and second filter device connecting stages 1560 having a screw hole opened at the center are provided at both sides of the filter mounting cover 156, respectively. After the primary filter 157, the secondary filter 158 and the tertiary filter 159 are coupled and mounted in the filter mounting cylinder 152, the first filtering device coupling block 1520 and the second filtering device coupling block 1560 are fastened by bolts. The filter mounting cap 156 is centrally formed with a circular through hole such that the inlet of the primary filter 157 passes through the circular through hole in the center of the filter mounting cap 156, thus facilitating direct sample introduction by the user, and the primary filter 157, the secondary filter 158 and the tertiary filter 159 can be fixed in the filter mounting cylinder 152.
The lower part of the second sample cell 14 is conical, so that the liquid in the second sample cell can be conveniently emptied when the second sample cell is drained. The second sample cell 14 is provided with a second sample cell inlet 142 at the top, a second sample cell outlet 144 at the bottom, and a second vent 143 at the top for communicating with the atmosphere to ensure stable gas pressure inside the second sample cell 14.
The multi-channel switching valve 38 has eight liquid inlets and one multi-channel switching valve liquid outlet 380, wherein the first multi-channel switching valve liquid inlet 381 is connected with a teflon tube passing through the methanol pool tube opening 181, and is finally connected to the liquid outlet of the methanol pool 161. The liquid inlet 382 of the second multi-channel switching valve is connected with a section of Teflon pipe which passes through the pipe orifice 180 of the pure water pool and is finally connected with the liquid outlet pipe of the pure water pool 160. The third multi-channel switching valve liquid inlet 383 is connected with a teflon tube which passes through the urine sample tube opening 150, and when the working mode of the instrument is urine detection, the teflon tube is finally connected to the first sample pool liquid outlet 155. The fourth multi-channel switching valve inlet 384 is connected to a teflon tube passing through the saliva sample tube port 151, and when the operating mode of the apparatus is saliva detection, the teflon tube will be finally connected to the first sample cell outlet 155. The liquid inlet 385 of the fifth multi-channel switching valve is connected with a section of Teflon tube which penetrates through the pipe orifice 182 of the dilute sulfuric acid pool and is finally connected to the liquid outlet pipe of the dilute sulfuric acid pool 162, the liquid inlet 386 of the sixth multi-channel switching valve is connected with a section of Teflon tube which penetrates through the pipe orifice 184 of the antibody solution pool and is finally connected to the liquid outlet pipe of the antibody solution pool 164, the liquid inlet 387 of the seventh multi-channel switching valve is connected with a section of Teflon tube which penetrates through the pipe orifice 185 of the developing solution pool and is finally connected to the liquid outlet pipe of the developing solution pool 165, and the liquid inlet 388 of the eighth multi-channel switching valve is communicated with air. The multi-channel switching valve 38 can select one inlet to communicate with the multi-channel switching valve outlet 380 according to the instruction of the first lower computer 53, and the other inlets are closed, and when the multi-channel switching valve is in a non-working state, the multi-channel switching valve outlet 380 is not communicated with any inlet.
The multi-channel switching valve liquid outlet 380 is connected with a section of Teflon pipe which passes through the first pipe orifice 170 of the extraction column and is finally connected to the liquid inlet of the extraction column 17, and the liquid outlet of the extraction column 17 is connected with a section of Teflon pipe which passes through the second pipe orifice 171 of the extraction column and is finally connected to the liquid inlet of the pretreatment peristaltic pump 360. The two-position three-way solenoid valve 34 has a first outlet, a second outlet and a fluid inlet. The two-position three-way electromagnetic valve 34 can select a liquid inlet of the two-position three-way electromagnetic valve 34 to be communicated with one liquid outlet according to the instruction of the first lower computer 53. A liquid outlet of the pretreatment peristaltic pump 360 is connected to a liquid inlet of a two-position three-way electromagnetic valve 34 through a section of Teflon pipe, a first liquid outlet of the two-position three-way electromagnetic valve 34 is connected to a liquid inlet of a first one-way valve 310 through a section of Teflon pipe, a liquid outlet of the first one-way valve 310 is connected to a first joint 320 of a liquid path four-way joint through a section of Teflon pipe, a fourth joint 323 of the liquid path four-way joint is connected with a section of Teflon pipe which penetrates through a pipe orifice 187 of the second waste liquid tank, and finally is connected to a liquid inlet pipe of the second waste liquid tank 167; the second liquid outlet of the two-position three-way electromagnetic valve 34 is connected with a teflon tube which passes through the second sample cell first pipe orifice 140 and is finally connected to the second sample cell liquid inlet 142, the second sample cell liquid outlet 144 is connected with a teflon tube which passes through the second sample cell second pipe orifice 141 and is finally connected to the liquid inlet of the first two-position two-way valve 330, the liquid outlet of the first two-position two-way valve 330 is connected to the liquid inlet of the first micro plunger pump 370 through a teflon tube, the liquid outlet of the first micro plunger pump 370 is connected to the liquid inlet of the second two-position two-way valve 331 through a teflon tube, and the liquid outlet of the second two-position two-way valve 331 is connected with a teflon tube which passes through the first dropping tube passage 191 and is finally connected to the sample dropping tube 435. This connection allows the treated intermediate solution to flow the unusable portion to the second waste reservoir 167 via the pretreatment peristaltic pump 360, to flow the usable portion to the second sample reservoir 14, and further to be transferred to the sample dropper 435 via the first micro-plunger pump 370 for sample application. The first one-way valve 310 can ensure that the useless part of the treated intermediate solution flows to the liquid path four-way joint 32 in one way and further flows into the second waste liquid pool 167, and the waste liquid backflow pollution sample is avoided when the pre-treatment peristaltic pump 360 does not work. When the first micro plunger pump 370 absorbs liquid, the first two-position two-way valve 330 is opened, the second two-position two-way valve 331 is closed, when the first micro plunger pump 370 drains liquid, the first two-position two-way valve 330 is closed, and the second two-position two-way valve 331 is opened, so that high-precision sample adding is realized conveniently.
The liquid outlet pipe of the diluent pool 163 is connected with a section of teflon pipe which penetrates through a diluent pool pipe orifice 183 and is finally connected to a liquid inlet of a third two-position two-way electromagnetic valve 332, a liquid outlet of the third two-position two-way electromagnetic valve 332 is connected to a liquid inlet of a second micro plunger pump 371 through a section of teflon pipe, a liquid outlet of the second micro plunger pump 371 is connected to a liquid inlet of a fourth two-position two-way electromagnetic valve 333 through a section of teflon pipe, a liquid outlet of the fourth two-position two-way electromagnetic valve 333 is connected with a section of teflon pipe which penetrates through a second dropper channel 192 and is finally connected to a diluent dropper 436. When the second micro plunger pump 371 sucks liquid, the third two-position two-way valve 332 is opened, the fourth two-position two-way valve 333 is closed, when the second micro plunger pump 371 discharges liquid, the third two-position two-way valve 332 is closed, and the fourth two-position two-way valve 333 is opened, so that high-precision sample adding is convenient to realize.
The liquid outlet pipe of the plate washing liquid pool 166 is connected with a Teflon pipe which penetrates through a pipe orifice 186 of the plate washing liquid pool and is finally connected to a liquid inlet of a plate washing liquid adding peristaltic pump 361, and a liquid outlet of the plate washing liquid adding peristaltic pump 361 is connected with a Teflon pipe which penetrates through a third dropper channel 193 and is finally connected to a plate washing liquid adding dropper 433. When the plate washing liquid adding peristaltic pump 361 works, the plate washing liquid is conveyed from the plate washing liquid pool 166 to the plate washing liquid adding dropper 433, and the liquid adding of the plate washing liquid is completed.
The plate washing liquid sucking dropper 438 is connected with a section of teflon tube which passes through the fourth dropper channel 194 and is finally connected to the liquid inlet of the plate washing liquid sucking peristaltic pump 362, the liquid outlet of the plate washing liquid sucking peristaltic pump 362 is connected to the liquid inlet of the second check valve 311 through a section of teflon tube, and the liquid outlet of the second check valve 311 is connected to the liquid path four-way connector second connector 321 through a section of teflon tube. The plate washing liquid suction peristaltic pump 362 can transport the plate washing waste liquid to the liquid path four-way joint 32 and then to the second waste liquid pool 167 when in operation. The second check valve 311 can ensure that the waste liquid can only flow in one direction, thereby avoiding sample contamination.
The waste liquid pipette 49 is connected with a section of teflon tube passing through the fifth pipette channel 195 and finally connected to the liquid inlet of the waste liquid pool peristaltic pump 363, the liquid outlet of the waste liquid pool peristaltic pump 363 is connected to the liquid inlet of the third check valve 312 through a section of teflon tube, and the liquid outlet of the third check valve 312 is connected to the liquid path four-way joint third joint 322 through a section of teflon tube. When the waste liquid pool liquid suction peristaltic pump 363 works, waste liquid in the first waste liquid pool 44 can be transported to the second waste liquid pool 167, and the third one-way valve 312 can ensure that the waste liquid can only flow in one direction to avoid backflow of the waste liquid when the waste liquid pool liquid suction peristaltic pump 363 does not work.
The working process of the invention is as follows:
(1) sufficient pure water, methanol solution, dilute sulfuric acid solution, antibody solution developing solution and plate washing solution are added into the pure water pool 160, the methanol pool 161, the dilute sulfuric acid pool 162, the diluent pool 163, the antibody solution pool 164, the developing solution pool 165 and the plate washing solution pool 166, respectively. The microplate 454 to which the reaction solution has been added is placed in the microplate holder 453, and the instrument top cover 2 is attached, and the power switch 59 is turned on.
(2) When the operation mode is urine calibration, the second sample cell 14 is replaced with a new one, and a sufficient standard solution is injected into the second sample cell 14 from the second vent 143 of the second sample cell 14 using a syringe. And selecting a urine calibration mode in an upper computer interface displayed by the touch screen display component 20, inputting parameters prompted by the upper computer, and clicking a start button. The upper computer transmits the working parameters to the first lower computer 53 and the second lower computer 54, respectively.
(2.1) under the control of the first lower machine 53, the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the ELISA plate support 453 to move leftwards until the ELISA plate support 453 touches the travel switch 46 and then stops. At this time, the first two-position two-way valve 330 is opened, the second two-position two-way valve 331 is closed, and the first micro plunger pump 370 performs a liquid sucking operation. This step is defined as the pipetting process of the first micro-plunger pump 370.
(2.2) the first two-position two-way valve 330 is closed, the second two-position two-way valve 331 is opened, and the first micro plunger pump 370 performs a liquid discharge action. This step is defined as the draining process of the first micro-plunger pump 370.
(2.3) the standard solution in the second sample cell 14 is sucked into the liquid path until the air in the teflon tube connected to the first micro-plunger pump 370 is completely exhausted and the standard solution stops when it starts to drop from the sample dropping tube 435. Subsequently, the third two-position two-way valve 332 is opened, the fourth two-position two-way valve 333 is closed, and the second micro plunger pump 371 performs a liquid suction operation. This step is defined as a pipetting process of the second micro plunger pump 371.
(2.4) the third two-position two-way valve 332 is closed, the fourth two-position two-way valve 333 is opened, and the second micro plunger pump 371 performs a liquid discharge action. This step is defined as the draining process of the second micro-plunger pump 371.
(2.5) the diluent in the diluent pool 163 is sucked into the liquid path until the air in the teflon tube connected to the second micro-plunger pump 371 is completely exhausted, and the diluent stops when the diluent begins to drop from the diluent dropping tube 436.
(2.6) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the ELISA plate support 453 to move rightwards until the rightmost sample cell of the ELISA plate 454 is just positioned right below the sample dropper 435, then the first micro peristaltic pump 370 performs a liquid discharge process, and standard solution is added into the sample cell according to the amount specified by the first lower computer 53. Then the first lead screw stepping motor 452 continues to rotate, so that the first lead screw sliding table 451 drives the microplate holder 453 to move 180mm rightward, at this time, the rightmost sample cell of the microplate 454 is just positioned right below the diluent dropper 436, and the second sample cell of the microplate 454 counted from right to left is just positioned right below the sample dropper 435. At this time, the second micro-plunger pump 371 performs a liquid discharge process, and adds a diluent to the sample cell in an amount prescribed by the first lower computer 53, so that the added standard solution and the diluent are mixed into a solution of a first set of concentration prescribed in the upper computer. And the first micro plunger pump 370 carries out liquid discharge again, adds standard solution into the second sample cell of the enzyme label plate 454 from right to left according to the amount specified by the first lower computer 53, and repeats the above processes until all the specified sample cells are matched with the solution with corresponding concentration according to the concentration gradient specified by the upper computer. In the whole process, the pipetting process is automatically performed when the solution in the first micro plunger pump 370 or the second micro plunger pump 371 is exhausted.
(2.7) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate support 453 to move, and the first sample cell on the rightmost side of the microplate 454 is moved to be right above the ultraviolet light source 47 and then stops. At this time, under the control of the second lower computer 54, the ultraviolet light source 47 emits light with a specific intensity, the light penetrates through a hole of 1mm of the elisa plate support 453, passes through the bottom of the elisa plate 454 and the solution in the elisa plate 454, and then enters the photodiode 423, and the photodiode 423 transmits the measured light intensity signal to the upper computer through the second lower computer 54 for storage, so that the citric acid detection of one sample is completed. The detection module 4 will then repeat the above process until the citric acid detection of the other samples to be detected is completed.
(2.8) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate support 453 to move, and the first sample cell on the rightmost side of the microplate 454 is moved to be right above the visible light source 48 and then stops. At this time, under the control of the second lower computer 54, the visible light source 48 emits light with a specific intensity, the light penetrates through a hole of 1mm of the elisa plate support 453, passes through the bottom of the elisa plate 454 and the solution in the elisa plate 454, enters the spectrometer 412, and the spectrometer 412 transmits the measured light intensity signal to the upper computer through the second lower computer 54 for storage, so that the oxalic acid detection of one sample is completed. And then the detection module 4 repeats the above processes until the oxalic acid detection of other samples to be detected is completed.
(2.9) the upper computer arranges all the measured data into calibration curves to be displayed in the touch screen display component 20 and stores the calibration curves.
(3) When the working mode is saliva calibration, the extraction column 17 is detached and the Teflon pipes of the liquid inlet and the liquid outlet are connected together by pipeline joints. The second sample cell 14 is replaced with a new one and sufficient standard solution is injected into the second sample cell 14 from the second vent 143 of the second sample cell 14 using a syringe. And selecting a saliva calibration mode in an upper computer interface displayed by the touch screen display component 20, inputting parameters prompted by the upper computer, and clicking a start button. The upper computer transmits the working parameters to the first lower computer 53 and the second lower computer 54, respectively.
(3.1) under the control of the first lower machine 53, the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the ELISA plate support 453 to move leftwards until the ELISA plate support 453 touches the travel switch 46 and then stops. At this time, the first micro plunger pump 370 performs a pipetting process and then a draining process. The standard solution in the second sample cell 14 is sucked into the liquid path until the air in the teflon tube connected to the first micro-plunger pump 370 is completely exhausted, and the standard solution stops when the standard solution starts to drip from the sample dripping tube 435. Next, the second micro-plunger pump 371 performs a liquid suction process and then performs a liquid discharge process. The diluent in the diluent reservoir 163 is sucked into the liquid path until the air in the teflon tube connected to the second micro-plunger pump 371 is completely exhausted, and the diluent stops when the diluent begins to drop from the diluent dropping tube 436.
(3.2) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the ELISA plate support 453 to move rightwards until the rightmost sample cell of the ELISA plate 454 is just positioned right below the sample dropper 435, then the first micro peristaltic pump 370 performs a liquid discharge process, and standard solution is added into the sample cell according to the amount specified by the first lower computer 53. Then the first lead screw stepping motor 452 continues to rotate, so that the first lead screw sliding table 451 drives the microplate holder 453 to move 180mm rightward, at this time, the rightmost sample cell of the microplate 454 is just positioned right below the diluent dropper 436, and the second sample cell of the microplate 454 counted from right to left is just positioned right below the sample dropper 435. At this time, the second micro-plunger pump 371 performs a liquid discharge process, and adds a diluent to the sample cell in an amount prescribed by the first lower computer 53, so that the added standard solution and the diluent are mixed into a solution of a first set of concentration prescribed in the upper computer. And the first micro plunger pump 370 carries out liquid discharge again, adds standard solution into the second sample cell of the enzyme label plate 454 from right to left according to the amount specified by the first lower computer 53, and repeats the above processes until all the specified sample cells are matched with the solution with corresponding concentration according to the concentration gradient specified by the upper computer. In the whole process, the pipetting process is automatically performed when the solution in the first micro plunger pump 370 or the second micro plunger pump 371 is exhausted. Subsequently, the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the elisa plate support 453 to move leftward until the elisa plate support 453 touches the travel switch 46 and then stops. The microplate 454 was allowed to rest in this position for 30 minutes, waiting for the sample to fully react.
(3.3) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the ELISA plate bracket 453 to move rightwards, and the movement is stopped until the rightmost sample pool of the ELISA plate 454 is just positioned right below the plate washing liquid-adding dropper 433. At this time, the wash plate solution adding peristaltic pump 361 is operated to transport the wash plate solution in the wash plate solution reservoir 166 to the wash plate solution adding dropper 433, and to drop the wash plate solution into the sample reservoir directly below the dropper, and the operation is stopped after dropping about 0.3 mL. Then the first lead screw stepping motor 452 continues to rotate, so that the first lead screw sliding table 451 drives the elisa plate bracket 453 to move 180mm rightwards, at this time, the rightmost sample pool of the elisa plate 454 is just positioned under the plate washing liquid pipette 438, and the second sample pool of the elisa plate 454 counted from right to left is just positioned under the plate washing liquid pipette 433. The wash plate solution adding peristaltic pump 361 is operated to transport the wash plate solution in the wash plate solution reservoir 166 to the wash plate solution adding dropper 433 and drop it into the sample reservoir directly below it, and stops after dropping about 0.3 mL. The second lead screw stepping motor 4312 rotates to make the second lead screw sliding table 4311 drive the rack 437 of the plate washing liquid pipette drop tube to move downwards until the plate washing liquid pipette drop tube 438 extends to the bottom of the sample cell right below the rack. The wash solution suction peristaltic pump 362 is activated to transport all the wash solution in the sample well to the second waste reservoir 167 and then stopped. The second lead screw stepping motor 4312 rotates to make the second lead screw sliding table 4311 drive the rack 437 of the plate washing liquid pipette drop to move upward until the plate washing liquid pipette drop 438 returns to the original position. The detection module 4 will then repeat the above actions until all the specified wells have completed the dropping and sucking of the wash solution. This step is defined as the plate washing process.
(3.4) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the ELISA plate support 453 to move leftwards until the ELISA plate support 453 touches the travel switch 46 and then stops. The first micro-plunger pump 370 will repeat the pipetting and draining process until the entire solution in the second sample cell 14 is transported to the first waste cell 44. The multi-channel switching valve 38 connects the second multi-channel switching valve liquid inlet 382 with the multi-channel switching valve liquid outlet 380, and the pretreatment peristaltic pump 360 operates to transport the pure water in the pure water tank 160 to the second sample tank 14 until the second sample tank 14 is filled. The first micro-plunger pump 370 will repeat the pipetting and draining processes until all of the pure water in the second sample cell 14 is transported to the first waste liquid cell 44. During this time, when the liquid in the first waste liquid pool 44 reaches the two-thirds volume of the first waste liquid pool 44, the waste liquid pool peristaltic pump 363 operates to transport all the liquid in the first waste liquid pool 44 to the second waste liquid pool 167. This step is defined as the cleaning process.
(3.5) the multi-channel switching valve 38 communicates the sixth multi-channel switching valve inlet 386 with the multi-channel switching valve outlet 380, and the pretreatment peristaltic pump 360 operates to transport the antibody solution in the antibody solution tank 164 to the second sample tank 14, and stops when the amount reaches the amount specified by the first lower computer 53. The first lead screw stepping motor 452 rotates to drive the first lead screw sliding table 451 to drive the microplate support 453 to move rightwards, and the movement is stopped until the rightmost sample cell of the microplate 454 is just positioned under the sample dropper 435, and then the first micro peristaltic pump 370 performs a liquid discharge process, and an antibody solution is added into the sample cell according to the amount specified by the first lower computer 53. Then the first lead screw stepping motor 452 continues to rotate, so that the first lead screw sliding table 451 drives the microplate holder 453 to move 180mm rightwards, and the second sample cell of the microplate 454 counted from right to left is just positioned right below the sample dropper 435. The first micro-plunger pump 370 again performs the liquid discharge operation, and adds the antibody solution into the second sample cell of the microplate 454 from right to left in the amount specified by the first lower computer 53, and repeats the above process until all the specified sample cells are added with the antibody solution in the amount specified by the upper computer. Throughout the process, the pipetting process will be performed automatically when the solution in the first micro-plunger pump 370 is emptied. Subsequently, the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate holder 453 to move leftward until the microplate holder 453 touches the travel switch 46, and then the first lead screw stepping motor 452 stops. The microplate 454 is allowed to rest in this position for 30 minutes, waiting for the sample to fully react.
(3.6) the sensing module 4 performs the plate washing process and the rinsing process again. Then, the multi-channel switching valve 38 communicates the seventh multi-channel switching valve liquid inlet 387 with the multi-channel switching valve liquid outlet 380, and the pretreatment peristaltic pump 360 operates to transport the color developing solution in the color developing solution tank 165 to the second sample tank 14, and stops when the amount reaches the amount specified by the first lower computer 53. The first lead screw stepping motor 452 rotates to drive the first lead screw sliding table 451 to drive the microplate support 453 to move rightwards, and the movement is stopped until the rightmost sample cell of the microplate 454 is just positioned under the sample dropper 435, and then the first micro peristaltic pump 370 executes a liquid discharge process, and a chromogenic solution is added into the sample cell according to the amount specified by the first lower computer 53. Then the first lead screw stepping motor 452 continues to rotate, so that the first lead screw sliding table 451 drives the microplate holder 453 to move 180mm rightwards, and the second sample cell of the microplate 454 counted from right to left is just positioned right below the sample dropper 435. The first micro plunger pump 370 performs the liquid discharging action again, adds the color developing solution into the second sample cell of the microplate 454 from right to left according to the amount specified by the first lower computer 53, and repeats the above processes until all the specified sample cells are added with the color developing solution of the amount specified by the upper computer. Throughout the process, the pipetting process will be performed automatically when the solution in the first micro-plunger pump 370 is emptied. Subsequently, the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate holder 453 to move leftward until the microplate holder 453 touches the travel switch 46, and then the first lead screw stepping motor 452 stops. The microplate 454 was allowed to rest in this position for 30 minutes, waiting for the sample to fully react.
(3.7) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate support 453 to move, and the first sample cell on the rightmost side of the microplate 454 is moved to be right above the visible light source 48 and then stops. At this time, under the control of the second lower computer 54, the visible light source 48 emits light with a specific intensity, the light penetrates through a hole of 1mm of the elisa plate support 453, passes through the bottom of the elisa plate 454 and the solution in the elisa plate 454, and enters the spectrometer 412, and the spectrometer 412 transmits the measured light intensity signal to the upper computer through the second lower computer 54 for storage, so that the detection of the Cyfra21-1 protein of one sample is completed. The detection module 4 will then repeat the above process until the detection of Cyfra21-1 protein of other samples to be detected is completed.
And (3.8) the upper computer arranges all the measured data into calibration curves to be displayed in the touch screen display component 20 and stores the calibration curves.
(4) When the mode of operation is urine testing. The filter device 15, the second sample cell 14 and the extraction column 17 to which the solid phase extraction packing has been added are replaced with new ones. A section of teflon tube which passes through the urine sample tube orifice 150 and is connected with the third multi-channel switching valve liquid inlet 383 is connected with the first sample pool liquid outlet 155. A 5ml sample of urine is injected into filter apparatus 15 from the inlet of primary filter 157 using a syringe. The urine sample is filtered by the first filter 157, the second filter 158 and the third filter 159 in sequence and then stored in the first sample tank 154. The urine detection mode is selected from the upper computer interface displayed by the touch screen display component 20, the parameters prompted by the upper computer are input, and the start button is clicked. The upper computer transmits the working parameters to the first lower computer 53 and the second lower computer 54, respectively.
(4.1) under the control of the first lower machine 53, the multi-channel switching valve 38 firstly connects the first multi-channel switching valve liquid inlet 381 with the multi-channel switching valve liquid outlet 380, and the liquid inlet of the two-position three-way solenoid valve 34 is connected with the first liquid outlet of the two-position three-way solenoid valve 34 but not with the second liquid outlet. The pre-treatment peristaltic pump 360 is activated and about 5ml of the methanol solution in the methanol reservoir 161 will be transported to the extraction column 17 and slowly pass through the packing to activate the packing, and then the methanol solution will flow through the two-position three-way solenoid valve 34 to the second waste reservoir 167. Then, the pretreatment peristaltic pump 360 stops operating. The multi-channel switching valve 38 connects the second multi-channel switching valve liquid inlet 382 with the multi-channel switching valve liquid outlet 380, the pre-treatment peristaltic pump 360 is actuated, about 5ml of pure water in the pure water tank 160 will be transported to the extraction column 17 and slowly pass through the packing to dilute and drain the methanol solution in the pipeline, and then the pure water will flow to the second waste liquid tank 167 through the two-position three-way solenoid valve 34. Then, the pretreatment peristaltic pump 360 stops operating. The multi-channel switching valve 38 connects the eighth multi-channel switching valve liquid inlet 388 with the multi-channel switching valve liquid outlet 380, and the pretreatment peristaltic pump 360 starts to operate, so that about 5ml of air is introduced into the pipeline, and pure water in the pipeline is completely discharged into the second waste liquid tank 167. Then, the pretreatment peristaltic pump 360 stops operating. The multi-channel switching valve 38 connects the third multi-channel switching valve inlet 383 to the multi-channel switching valve outlet 380, the pre-treatment peristaltic pump 360 is actuated, and 5ml of the urine sample in the first sample tank 154 is transported to the extraction column 17 and slowly passes through the packing, which is enriched in the citric acid and oxalic acid in the urine and the other unwanted components flow into the second waste tank 167. Then, the pretreatment peristaltic pump 360 stops operating. The multi-channel switching valve 38 connects the fifth multi-channel switching valve inlet 385 with the multi-channel switching valve outlet 380, and the inlet of the two-position three-way solenoid valve 34 is connected to the second outlet of the two-position three-way solenoid valve 34 but not to the first outlet. The pre-treatment peristaltic pump 360 is activated and about 5ml of the dilute sulfuric acid sample in the dilute sulfuric acid tank 162 will be transported to the extraction column 17 and slowly through the packing, where the dilute sulfuric acid will dissolve the citric acid and oxalic acid enriched in the packing and flow into the second sample tank 14. Then, the pretreatment peristaltic pump 360 stops operating. The multi-channel switching valve 38 connects the eighth multi-channel switching valve liquid inlet 388 with the multi-channel switching valve liquid outlet 380, the pre-treatment peristaltic pump 360 starts to operate, and about 5ml of air is introduced into the pipeline, so that all the dilute sulfuric acid in the pipeline passes through the packing of the extraction column 17 and is finally stored in the second sample cell 14. The pre-treatment peristaltic pump 360 then stops.
(4.2) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the ELISA plate support 453 to move leftwards until the ELISA plate support 453 touches the travel switch 46 and then stops. At this time, the first micro plunger pump 370 performs a pipetting process and then a draining process. The sample solution in the second sample cell 14 is sucked into the liquid path until the air in the teflon tube connected to the first micro-plunger pump 370 is completely exhausted, and the sample solution stops when it starts to drip from the sample dripping tube 435.
(4.3) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate support 453 to move rightwards until the rightmost sample cell of the microplate 454 is just positioned right below the sample dropper 435, and then the first micro peristaltic pump 370 performs a liquid discharge process and adds a sample solution into the sample cell according to the amount specified by the first lower computer 53. Then the first lead screw stepping motor 452 continues to rotate, so that the first lead screw sliding table 451 drives the microplate holder 453 to move 180mm rightwards, and at this time, the second sample cell of the microplate 454 counted from right to left is just positioned right below the sample dropper 435. The first micro-plunger pump 370 performs the liquid discharging operation again, and the same amount of the sample solution is added to the second well of the microplate 454 from right to left, and the above process is repeated until the prescribed amount of the sample solution is added to all the prescribed wells. Throughout the process, the pipetting process will be performed automatically when the solution in the first micro-plunger pump 370 is emptied.
(4.4) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate support 453 to move, and the first sample cell on the rightmost side of the microplate 454 is moved to be right above the ultraviolet light source 47 and then stops. At this time, under the control of the second lower computer 54, the ultraviolet light source 47 emits light with a specific intensity, the light penetrates through a hole of 1mm of the elisa plate support 453, passes through the bottom of the elisa plate 454 and the solution in the elisa plate 454, and then enters the photodiode 423, and the photodiode 423 transmits the measured light intensity signal to the upper computer through the second lower computer 54 for storage, so that the citric acid detection of one sample is completed. The detection module 4 will then repeat the above process until the citric acid detection of the other samples to be detected is completed.
(4.5) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate support 453 to move, and the first sample cell on the rightmost side of the microplate 454 is moved to be right above the visible light source 48 and then stops. At this time, under the control of the second lower computer 54, the visible light source 48 emits light with a specific intensity, the light penetrates through a hole of 1mm of the elisa plate support 453, passes through the bottom of the elisa plate 454 and the solution in the elisa plate 454, enters the spectrometer 412, and the spectrometer 412 transmits the measured light intensity signal to the upper computer through the second lower computer 54 for storage, so that the oxalic acid detection of one sample is completed. And then the detection module 4 repeats the above processes until the oxalic acid detection of other samples to be detected is completed.
(4.6) the upper computer arranges all the measured data into calibration curves to be displayed in the touch screen display component 20 and stores the calibration curves.
(5) When the working mode is saliva detection, the new filtering device 15 and the second sample cell 14 are replaced, the extraction column 17 is detached, the teflon pipes of the liquid inlet and the liquid outlet are connected together through pipeline joints, and the section of the teflon pipe connected with the liquid inlet 384 of the fourth multi-channel switching valve, which penetrates through the saliva sample pipe port 151, is connected with the liquid outlet 155 of the first sample cell. A 5ml saliva sample was injected into the filter apparatus 15 from the inlet of the primary filter 157 using a syringe. The saliva sample is filtered by a primary filter 157, a secondary filter 158 and a tertiary filter 159 in sequence and then stored in a first sample reservoir 154. And selecting a saliva detection mode in an upper computer interface displayed by the touch screen display component 20, inputting parameters prompted by the upper computer, and clicking a start button. The upper computer transmits the working parameters to the first lower computer 53 and the second lower computer 54, respectively.
(5.1) the multi-way switching valve 38 communicates a fourth multi-way switching valve liquid inlet 384 with the multi-way switching valve liquid outlet 380, and a liquid inlet of the two-position three-way solenoid valve 34 communicates with a second liquid outlet of the two-position three-way solenoid valve 34 but does not communicate with the first liquid outlet. The pre-treatment peristaltic pump 360 is activated to transport all saliva samples in the first sample reservoir 154 to the second sample reservoir 14 for storage.
(5.2) under the control of the first lower machine 53, the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the ELISA plate support 453 to move leftwards until the ELISA plate support 453 touches the travel switch 46 and then stops. At this time, the first micro plunger pump 370 performs a pipetting process and then a draining process. The sample solution in the second sample cell 14 is sucked into the liquid path until the air in the teflon tube connected to the first micro-plunger pump 370 is completely exhausted, and the sample solution stops when it starts to drip from the sample dripping tube 435.
(5.3) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate support 453 to move rightwards until the rightmost sample cell of the microplate 454 is just positioned right below the sample dropper 435, and then the first micro peristaltic pump 370 performs a liquid discharge process and adds a sample solution into the sample cell according to the amount specified by the first lower computer 53. Then the first lead screw stepping motor 452 continues to rotate, so that the first lead screw sliding table 451 drives the microplate holder 453 to move 180mm rightwards, and at this time, the second sample cell of the microplate 454 counted from right to left is just positioned right below the sample dropper 435. The first micro-plunger pump 370 performs the liquid discharging operation again, and the same amount of the sample solution is added to the second well of the microplate 454 from right to left, and the above process is repeated until the prescribed amount of the sample solution is added to all the prescribed wells. Throughout the process, the pipetting process will be performed automatically when the solution in the first micro-plunger pump 370 is emptied.
(5.4) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the ELISA plate support 453 to move leftwards until the ELISA plate support 453 touches the travel switch 46 and then stops. The microplate 454 is allowed to rest in this position for 30 minutes, waiting for the sample to fully react.
(5.5) the sensing module 4 performs a plate washing process and a rinsing process.
(5.6) the multi-channel switching valve 38 communicates the sixth multi-channel switching valve inlet 386 with the multi-channel switching valve outlet 380, and the pretreatment peristaltic pump 360 is operated to transport the antibody solution in the antibody solution tank 164 to the second sample tank 14, and stops when the amount reaches the amount specified by the first lower computer 53. The first lead screw stepping motor 452 rotates to drive the first lead screw sliding table 451 to drive the microplate support 453 to move rightwards, and the movement is stopped until the rightmost sample cell of the microplate 454 is just positioned under the sample dropper 435, and then the first micro peristaltic pump 370 performs a liquid discharge process, and an antibody solution is added into the sample cell according to the amount specified by the first lower computer 53. Then the first lead screw stepping motor 452 continues to rotate, so that the first lead screw sliding table 451 drives the microplate holder 453 to move 180mm rightwards, and the second sample cell of the microplate 454 counted from right to left is just positioned right below the sample dropper 435. The first micro-plunger pump 370 again performs the liquid discharge operation, and adds the antibody solution into the second sample cell of the microplate 454 from right to left in the amount specified by the first lower computer 53, and repeats the above process until all the specified sample cells are added with the antibody solution in the amount specified by the upper computer. Throughout the process, the pipetting process will be performed automatically when the solution in the first micro-plunger pump 370 is emptied. Subsequently, the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate holder 453 to move leftward until the microplate holder 453 touches the travel switch 46, and then the first lead screw stepping motor 452 stops. The microplate 454 is allowed to rest in this position for 30 minutes, waiting for the sample to fully react.
(5.7) the sensing module 4 performs the plate washing process and the washing process again. Then, the multi-channel switching valve 38 communicates the seventh multi-channel switching valve liquid inlet 387 with the multi-channel switching valve liquid outlet 380, and the pretreatment peristaltic pump 360 operates to transport the color developing solution in the color developing solution tank 165 to the second sample tank 14, and stops when the amount reaches the amount specified by the first lower computer 53. The first lead screw stepping motor 452 rotates to drive the first lead screw sliding table 451 to drive the microplate support 453 to move rightwards, and the movement is stopped until the rightmost sample cell of the microplate 454 is just positioned under the sample dropper 435, and then the first micro peristaltic pump 370 executes a liquid discharge process, and a chromogenic solution is added into the sample cell according to the amount specified by the first lower computer 53. Then the first lead screw stepping motor 452 continues to rotate, so that the first lead screw sliding table 451 drives the microplate holder 453 to move 180mm rightwards, and the second sample cell of the microplate 454 counted from right to left is just positioned right below the sample dropper 435. The first micro plunger pump 370 performs the liquid discharging action again, adds the color developing solution into the second sample cell of the microplate 454 from right to left according to the amount specified by the first lower computer 53, and repeats the above processes until all the specified sample cells are added with the color developing solution of the amount specified by the upper computer. Throughout the process, the pipetting process will be performed automatically when the solution in the first micro-plunger pump 370 is emptied. Subsequently, the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate holder 453 to move leftward until the microplate holder 453 touches the travel switch 46, and then the first lead screw stepping motor 452 stops. The microplate 454 is allowed to rest in this position for 30 minutes, waiting for the sample to fully react.
(5.8) the first lead screw stepping motor 452 rotates to enable the first lead screw sliding table 451 to drive the microplate support 453 to move, and the first sample cell on the rightmost side of the microplate 454 is moved to be right above the visible light source 48 and then stops. At this time, under the control of the second lower computer 54, the visible light source 48 emits light with a specific intensity, the light penetrates through a hole of 1mm of the elisa plate support 453, passes through the bottom of the elisa plate 454 and the solution in the elisa plate 454, and enters the spectrometer 412, and the spectrometer 412 transmits the measured light intensity signal to the upper computer through the second lower computer 54 for storage, so that the detection of the Cyfra21-1 protein of one sample is completed. The detection module 4 will then repeat the above process until the detection of Cyfra21-1 protein of other samples to be detected is completed.
(5.9) the upper computer arranges all the measured data into calibration curves to be displayed in the touch screen display component 20 and stores the calibration curves.
When all the processes are completed, the instrument will automatically perform the cleaning process. After the cleaning process is finished, the liquids in the pure water pool 160, the methanol pool 161, the dilute sulfuric acid pool 162, the diluent pool 163, the antibody solution pool 164, the color development solution pool 165, the plate washing solution pool 166 and the second waste solution pool 167 are emptied, the elisa plate 454 is taken out, and finally the power switch 59 is turned off.
The above-described embodiments are intended to illustrate rather than to limit the invention, and any modifications and variations of the present invention are within the spirit of the invention and the scope of the appended claims.

Claims (7)

1. The utility model provides a full-automatic urine of multi-parameter and saliva pretreatment and detection integration instrument which characterized in that: the device comprises a case main body, a case top cover, a pretreatment module, a control module, a detection module and a liquid pool frame; the top cover of the case is arranged on the case main body; the top cover of the case is provided with a touch screen display component for man-machine interaction, and an upper computer is arranged in the touch screen display component; the pretreatment module, the control module and the detection module are arranged in the case main body, the control module is connected with the upper computer, and the pretreatment module and the detection module are connected with the control module;
the liquid pool frame is arranged on the outer side of the case main body and comprises eight liquid pool mounting grooves which are respectively provided with a pure water pool, a methanol pool, a dilute sulfuric acid pool, a diluent pool, an antibody solution pool, a developing solution pool, a plate washing liquid pool and a second waste liquid pool;
the pretreatment module comprises a multi-channel switching valve, a pretreatment peristaltic pump, a plate washing liquid adding peristaltic pump, a plate washing liquid sucking peristaltic pump, a waste liquid pool sucking peristaltic pump, a first micro plunger pump and a second micro plunger pump; the multi-channel switching valve is provided with eight liquid inlets and one liquid outlet, and the liquid inlets are respectively connected with a methanol pool, a pure water pool, a first sample pool, a dilute sulfuric acid pool, an antibody solution pool, a developing solution pool and air; the first sample pool stores urine or saliva, and different multi-channel switching valve liquid inlets are used when the urine or the saliva is detected; the liquid outlet is connected to a liquid inlet of the pretreatment peristaltic pump through an extraction column; a liquid outlet of the pretreatment peristaltic pump is connected with a liquid inlet of a two-position three-way electromagnetic valve; the two-position three-way electromagnetic valve is provided with two liquid outlets which are respectively connected to the liquid inlets of the second waste liquid pool and the second sample pool; the liquid outlet of the second sample cell is connected with the liquid inlet of the first miniature plunger pump; a liquid outlet of the first miniature plunger pump is connected with a sample dropper in the detection module; the liquid inlet of the second miniature plunger pump is connected with the diluent pool, and the liquid outlet of the second miniature plunger pump is connected with the diluent drop pipe of the detection module; a liquid inlet of the plate washing liquid adding peristaltic pump is connected with the plate washing liquid pool, and a liquid outlet of the plate washing liquid adding peristaltic pump is connected with a plate washing liquid adding dropper of the detection module;
the detection module comprises a spectrometer, a photodiode, a dropper mounting device, a first waste liquid pool and a first lead screw module; the dropper mounting device comprises a dropper mounting support, a second lead screw module and a plate washing liquid imbibing dropper bracket; the dropper mounting support is provided with a plate washing liquid adding dropper, a movable through hole of the plate washing liquid absorbing dropper, a sample dropper and a diluent dropper; the second lead screw module is arranged on the dropper mounting support; the washing plate liquid imbibition burette bracket is arranged on the second screw rod module, the washing plate liquid imbibition burette is arranged on the washing plate liquid imbibition burette bracket, passes through the movable through hole of the washing plate liquid imbibition burette, and can move up and down under the drive of the second screw rod module and the limitation of the movable through hole of the washing plate liquid imbibition burette; an ELISA plate bracket with an ELISA plate is arranged on the first lead screw module, and the first waste liquid pool is arranged below the four droppers; the plate washing liquid imbibition dropper is connected with a liquid inlet of the plate washing liquid imbibition peristaltic pump; a liquid outlet of the plate washing liquid suction peristaltic pump is connected to the second waste liquid pool; the first waste liquid pool is connected with a liquid inlet of a waste liquid pool liquid suction peristaltic pump, and a liquid outlet of the waste liquid pool liquid suction peristaltic pump is connected with the second waste liquid pool; the spectrometer is used for detecting the absorbance of oxalic acid in the urine sample in the ELISA plate and the absorbance of Cyfra21-1 protein in the saliva sample, and the photodiode is used for detecting the absorbance of citric acid in the urine sample in the ELISA plate;
the control module is used for receiving a control instruction of the upper computer, controlling the first lead screw module and the second lead screw module in the detection module to move, controlling the liquid inlet switching of a multi-channel switching valve in the pretreatment module, controlling the liquid outlet switching of a two-position three-way electromagnetic valve, and controlling the work of the pretreatment peristaltic pump, the plate washing liquid adding peristaltic pump, the plate washing liquid peristaltic pump, the waste liquid pool peristaltic pump, the first miniature plunger pump and the second miniature plunger pump; and simultaneously, receiving signal data of the spectrograph and the photodiode and feeding back the signal data to the upper computer.
2. The multi-parameter full-automatic integrated instrument for pretreatment and detection of urine and saliva according to claim 1, wherein: the pretreatment module comprises a pretreatment module mounting plate connected in the case main body, and the pretreatment module mounting plate is connected with a first two-position two-way electromagnetic valve, a second two-position two-way electromagnetic valve, a third two-position two-way electromagnetic valve, a fourth two-position two-way electromagnetic valve, a two-position three-way electromagnetic valve, a first motor driver for driving a first screw module, a second motor driver for driving a second screw module, a third motor driver for driving a pretreatment peristaltic pump, a fourth motor driver for driving a plate washing liquid adding peristaltic pump, a fifth motor driver for driving a plate washing liquid absorbing peristaltic pump and a sixth motor driver for driving a waste liquid pool absorbing peristaltic pump, wherein the first two-position two-way electromagnetic valve, the second two-position two-way electromagnetic valve, the third two-position two-way electromagnetic valve, the fourth two-position two-way electromagnetic valve and the two-position three-way electromagnetic valve are used for controlling liquid paths; the pretreatment module mounting plate is also provided with a peristaltic pump mounting plate, a plunger pump mounting plate, a switching valve mounting bracket and a first hydroelectric partition plate for separating a liquid path and a circuit; the peristaltic pump mounting plate is connected with a pretreatment peristaltic pump for sample pretreatment, a plate washing liquid adding peristaltic pump for dropwise adding a plate washing liquid to an ELISA plate, a plate washing liquid sucking peristaltic pump for sucking the plate washing liquid from the ELISA plate and a waste liquid tank sucking peristaltic pump for sucking a waste liquid from a first waste liquid tank; the plunger pump mounting plate is provided with a first micro plunger pump for adding a sample to the detection module and a second micro plunger pump for adding a diluent to the detection module; the switching valve mounting bracket is provided with a multi-channel switching valve; the first hydroelectric partition plate is provided with 4 oval through holes for circuit connection of the peristaltic pump and the motor drivers, and each motor driver is connected with the control module.
3. The multi-parameter fully-automatic integrated instrument for pre-treatment and detection of urine and saliva according to claim 1, characterized in that: the control module is provided with a photodiode driver for driving a photodiode, a spectrometer driver for driving a spectrometer, a light source driver for driving an ultraviolet light source and a visible light source, and a first lower computer for transmitting instructions to the first motor driver, the second motor driver, the third motor driver, the fourth motor driver, the fifth motor driver, the sixth motor driver, the plunger pump driver, the multi-channel switching valve and the five-way relay module; the second lower computer is used for transmitting instructions to the photodiode driver, the spectrometer driver and the light source driver, and the switching power supply is used for supplying power to all electric equipment; the first lower computer and the second lower computer can be communicated with an upper computer in the touch screen display assembly.
4. The multi-parameter fully-automatic integrated instrument for pre-treatment and detection of urine and saliva according to claim 1, characterized in that: the detection module also comprises a travel switch mounting support, an ultraviolet light source mounting support, a visible light source mounting support, a spectrometer mounting device and a photodiode mounting device;
the travel switch mounting support is provided with two waist-shaped holes and a travel switch for feeding back the position of the ELISA plate bracket, the ultraviolet light source mounting support is provided with a 340nm single-wavelength ultraviolet light source, and the visible light source mounting support is provided with a visible light source with the wavelength range of 390nm-700 nm; the ultraviolet light source and the visible light source are controlled by the control module to emit light;
the spectrometer mounting device comprises a spectrometer mounting support arranged on the detection module mounting plate, a spectrometer mounting groove is formed in the spectrometer mounting support, the spectrometer is mounted in the spectrometer mounting groove, a first light-transmitting hole is formed in the lower portion of the spectrometer mounting groove, spectrometer positioning threaded holes and spectrometer positioning screws for adjusting and fixing the position of the spectrometer are arranged on the left side and the right side of the spectrometer mounting groove, and the first light-transmitting hole and the visible light source are coaxial;
the photodiode mounting device comprises a photodiode mounting support and a photodiode gland, wherein the photodiode mounting support is provided with a photodiode mounting groove, the photodiode is mounted in the photodiode mounting groove, a second light-transmitting hole is formed in the photodiode mounting groove, and the second light-transmitting hole and the ultraviolet light source are coaxial; the photodiode mounting support is provided with a first left pressing screw hole and a first right pressing screw hole which are used for fastening the photodiode; the center of the photodiode gland is provided with a waist-shaped hole, and two sides of the photodiode gland are provided with a second left pressing screw hole and a second right pressing screw hole for fastening the photodiode;
5. the multi-parameter fully-automatic integrated instrument for pre-treatment and detection of urine and saliva according to claim 1, characterized in that: the bottom surface of the primary waste liquid pool is an inclined plane inclined towards the right rear vertex, and a waste liquid suction pipe is connected to the right rear vertex of the bottom surface of the primary waste liquid pool in an adhesive mode.
6. The multi-parameter full-automatic integrated instrument for pretreatment and detection of urine and saliva according to claim 1, wherein: the outer side of the case main body is also provided with a filtering device, the filtering device comprises a filter mounting cylinder, a first sample tank, a filter mounting cover, a first-stage filter, a second-stage filter and a third-stage filter, the side wall of the filter mounting cylinder is provided with a first vent hole for communicating with the atmosphere, a first sample tank liquid outlet is arranged below the first sample tank, the first sample tank liquid outlet is connected with a multi-channel switching valve liquid inlet, the first sample tank is fixedly connected below the filter mounting cylinder, and a filter partition plate with a circular hole in the center is arranged between the filter mounting cylinder and the first sample tank; the filter comprises a primary filter, a secondary filter, a tertiary filter and a filter partition plate, wherein a 0.8um filter membrane is arranged in the primary filter, a 0.45um filter membrane is arranged in the secondary filter, a 0.22um filter membrane is arranged in the tertiary filter, an outlet of the primary filter is connected to an inlet of the secondary filter, an outlet of the secondary filter is connected to an inlet of the tertiary filter, the tertiary filter is arranged on the filter partition plate, and an outlet of the tertiary filter penetrates through a circular hole in the center of the filter partition plate; a circular through hole is formed in the center of the filter mounting cover, and an inlet of the primary filter penetrates through the circular through hole in the center of the filter mounting cover;
the lower part of the second sample cell is conical, a second sample cell liquid inlet and a second vent hole used for communicating atmosphere are arranged at the top of the second sample cell, and a second sample cell liquid outlet is arranged at the bottom of the second sample cell.
7. The multi-parameter fully-automatic integrated instrument for pre-treatment and detection of urine and saliva according to claim 1, characterized in that: the pretreatment peristaltic pump, the plate washing liquid suction peristaltic pump, the waste liquid pool liquid suction peristaltic pump and the second waste liquid pool are connected through a liquid path four-way joint, and one-way valves are arranged on pipelines among the pretreatment peristaltic pump, the plate washing liquid suction peristaltic pump, the waste liquid pool liquid suction peristaltic pump and the liquid path four-way joint.
CN202210265613.2A 2022-03-17 2022-03-17 Multi-parameter full-automatic urine and saliva pretreatment and detection integrated instrument Pending CN114705878A (en)

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CN202210265613.2A CN114705878A (en) 2022-03-17 2022-03-17 Multi-parameter full-automatic urine and saliva pretreatment and detection integrated instrument

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CN202210265613.2A CN114705878A (en) 2022-03-17 2022-03-17 Multi-parameter full-automatic urine and saliva pretreatment and detection integrated instrument

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11761885B1 (en) * 2023-05-05 2023-09-19 King Faisal University Device for determining fish gender

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11761885B1 (en) * 2023-05-05 2023-09-19 King Faisal University Device for determining fish gender

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