CN114236161A - Liquid suction structure and liquid supply device for immunoassay - Google Patents
Liquid suction structure and liquid supply device for immunoassay Download PDFInfo
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- CN114236161A CN114236161A CN202111669153.1A CN202111669153A CN114236161A CN 114236161 A CN114236161 A CN 114236161A CN 202111669153 A CN202111669153 A CN 202111669153A CN 114236161 A CN114236161 A CN 114236161A
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- 239000007788 liquid Substances 0.000 title claims abstract description 93
- 238000003018 immunoassay Methods 0.000 title claims abstract description 17
- 238000006243 chemical reaction Methods 0.000 claims abstract description 68
- 238000005406 washing Methods 0.000 claims abstract description 59
- 238000004140 cleaning Methods 0.000 claims abstract description 42
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 27
- 238000001802 infusion Methods 0.000 claims abstract description 27
- 230000003139 buffering effect Effects 0.000 claims abstract description 4
- 230000007246 mechanism Effects 0.000 claims description 52
- 230000001360 synchronised effect Effects 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims 4
- 239000013589 supplement Substances 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 description 7
- 238000005192 partition Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 238000004020 luminiscence type Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000000504 luminescence detection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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Abstract
The present invention provides a liquid uptake structure for immunoassay, comprising: sucking a needle; the buffer assembly comprises a reaction cup seat, a needle washing groove, a plurality of buffer cups and a plurality of infusion pipelines; wherein, the reaction cup seat is used for loading a reaction cup containing the primary reaction liquid; the needle washing groove is communicated with an infusion pipeline and is used for introducing washing liquid into the needle washing groove to clean the suction needle; at least two buffer cups are respectively communicated with a transfusion pipeline for leading in and buffering reaction or cleaning liquid; during suction, the suction needle extends into the reaction cup or the buffer cup to suck liquid; when cleaning, the suction needle extends into the needle washing groove to clean the needle body. The invention also provides a liquid supply device. The suction needle is matched with the buffer assembly to realize the suction of the primary reaction liquid or the cleaning liquid and the cleaning work of the suction needle, so that the suction efficiency is improved. In addition, a plurality of infusion pipelines are arranged to supplement liquid for reaction or liquid for cleaning into the buffer cup in time so as to realize continuous suction work.
Description
Technical Field
The invention belongs to the technical field of medical detection equipment, and particularly relates to a liquid suction structure and a liquid supply device for immunoassay.
Background
In the field of medical and biological engineering, there are a variety of automated analyzers that use a liquid such as blood, serum, or urine as a sample, detect a specific biological component or chemical substance contained in the sample, and have an important guidance function for diagnosis and prevention of diseases. Automated analyzers continuously perform analysis cycles and require the use of a pipette needle to aspirate multiple liquids in one analysis cycle. In order to shorten the time of the analysis cycle and improve the efficiency of the automated analyzer, it is necessary to move the liquid container reasonably to achieve efficient liquid suction.
Disclosure of Invention
In order to achieve the above object, the present invention is achieved by the following technical solutions.
The present invention provides a liquid uptake structure for immunoassay, comprising:
a suction needle for sucking liquid;
the buffer assembly comprises a reaction cup seat, a needle washing groove, a plurality of buffer cups and a plurality of infusion pipelines;
the reaction cup seat is used for loading a reaction cup containing a primary reaction liquid; the needle washing groove is communicated with the infusion pipeline and is used for introducing washing liquid into the needle washing groove to clean the suction needle; at least two buffer cups are respectively communicated with one infusion pipeline and used for introducing and buffering reaction or cleaning liquid;
during suction, the suction needle extends into the reaction cup or the buffer cup to suck liquid; when cleaning, the suction needle extends into the needle washing groove to clean the needle body.
Preferably, the buffer assembly further comprises a base; the reaction cup seat, the needle washing groove and the plurality of buffer cups are arranged on the base.
Preferably, the base is provided with a plurality of clamping grooves; the buffer cup is clamped in the clamping groove.
Preferably, the method further comprises the following steps:
a rotating mechanism connected to the cache assembly;
a lifting mechanism connected to the suction needle;
the buffer memory component is driven by the rotating mechanism to rotate horizontally, so that the reaction cup seat or the needle washing groove or the buffer memory cup is positioned in the working area of the suction needle; the lifting mechanism drives the suction needle downwards to be inserted into a cup or a groove in a working area.
Preferably, the device further comprises a fixed seat, and the rotating mechanism is mounted on the fixed seat; the rotating mechanism is a synchronous belt transmission mechanism, one end of the rotating shaft of the driven wheel is rotatably connected to the fixed seat, and the other end of the rotating shaft of the driven wheel is coaxially connected to the cache assembly.
Preferably, a baffle is sleeved on the periphery of the rotating shaft of the driven wheel, and a correlation type photoelectric sensor is arranged on the fixed seat; the baffle plate and the correlation type photoelectric sensor are matched to position a rotation stop point of the cache assembly.
Preferably, the baffle is of an annular structure and is provided with a plurality of notches; the plurality of gaps are respectively corresponding to the positions of the reaction cup seat or the needle washing groove or the cache cup.
Preferably, the lifting mechanism comprises a fixing plate, a first motor, a screw rod and a sleeve which are matched; the screw rod is coaxially connected to the output shaft of the first motor; the fixing plate is respectively fixed on the sleeve and the suction needle;
the first motor drives the screw rod to rotate, so that the sleeve drives the suction needle to move along the screw rod.
Preferably, two through holes are formed in the bottom of the needle washing groove and used for being communicated with the infusion pipeline and the liquid discharge pipeline respectively.
The present invention also provides a liquid supply apparatus comprising:
a liquid take-up structure for immunoassay as described above;
one end of the conveying pipeline is communicated with the suction needle, and the other end of the conveying pipeline is communicated with the measuring pool;
and the plunger pump is used for forming negative pressure, so that the suction needle sucks liquid and then leads the liquid into the measuring pool through the conveying pipeline.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a liquid suction structure for immunodetection, which is characterized in that a buffer assembly is provided with a reaction cup seat, a needle washing groove, a plurality of buffer cups and a plurality of infusion pipelines, wherein the reaction cup seat is used for loading the reaction cups filled with primary reaction liquid, at least two buffer cups are respectively used for containing reaction or cleaning liquid, and a suction needle is matched with the buffer assembly to realize suction of the primary reaction liquid or the cleaning liquid and cleaning of the suction needle, so that the suction efficiency is improved. In addition, a plurality of infusion pipelines are arranged to supplement liquid for reaction or liquid for cleaning into the buffer cup in time so as to realize continuous suction work.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings. The detailed description of the present invention is given in detail by the following examples and the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic view of an assembled three-dimensional structure of a buffer assembly, a rotating mechanism and a fixing seat of the present invention;
FIG. 2 is a top view of an assembly structure of the buffer assembly, the rotating mechanism and the fixing base of the present invention;
FIG. 3 is a schematic perspective view of the assembly of the suction structure body and the housing of the present invention;
FIG. 4 is an enlarged view taken at A in FIG. 3;
FIG. 5 is a side view of an assembly structure of the supply device body, the measuring cell and the liquid outlet pipe according to the present invention.
In the figure: 100. a supply device body;
101. a suction structure body; 10. sucking a needle; 20. a cache component; 21. a reaction cup seat; 22. a needle washing groove; 23. caching a cup; 231. a first cache cup; 232. a second cache cup; 233. a third cache cup; 241. a first infusion line; 242. a second infusion pipeline; 243. a third infusion pipeline; 25. a base; 251. a card slot; 30. a rotation mechanism; 31. a driven wheel; 311. a driven wheel rotating shaft; 32. a synchronous belt; 33. a driving wheel; 34. a second motor; 40. a lifting mechanism; 41. a fixing plate; 42. a first motor; 43. a screw rod; 44. a sleeve; 45. a guide post; 50. a fixed seat; 61. a baffle plate; 611. a notch; 62. a correlation type photoelectric sensor; 70. a bearing; 80. a connecting plate;
102. a delivery line;
103. a plunger pump;
104. a housing; 1041. a partition plate; 10411. mounting holes;
200. a measuring cell;
300. and a liquid outlet pipeline.
Detailed Description
The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent to those skilled in the art from the following detailed description, which, taken in conjunction with the annexed drawings, discloses a full and partial embodiment of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The terms "first," "second," "primary," "secondary," and the like in the description and in the claims of the present application do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. Also, the use of the terms "a," "an," or "the" and similar referents do not denote a limitation of quantity, but rather denote the presence of at least one. The word "comprise" or "comprises", and the like, means that the element or item listed before "comprises" or "comprising" covers the element or item listed after "comprising" or "comprises" and its equivalents, and does not exclude other elements or items. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, and the like are used based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back. These relative terms are for convenience of description and are not generally intended to require a particular orientation. Terms concerning attachments, coupling and the like (e.g., "connected" and "attached") refer to a relationship wherein structures are secured or attached, either directly or indirectly, to one another through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that any combination of the embodiments or technical features described below can be used to form a new embodiment without conflict.
Example 1
The present invention provides a liquid suction structure for immunoassay, as shown in fig. 1 and fig. 2, comprising a suction structure body 101, wherein the suction structure body 101 comprises:
a suction needle 10 for sucking a liquid;
the buffer component 20 comprises a reaction cup seat 21, a needle washing groove 22, a plurality of buffer cups 23 and a plurality of infusion pipelines;
wherein, the reaction cup seat 21 is used for loading a reaction cup containing a primary reaction liquid; the needle washing groove 22 is communicated with the infusion pipeline and is used for introducing washing liquor into the needle washing groove 22 so as to clean the suction needle 10; at least two buffer cups 23 are respectively communicated with one infusion pipeline for introducing and buffering reaction or cleaning liquid;
during suction, the suction needle 10 extends into the reaction cup or the buffer cup 23 to suck liquid; when cleaning, the suction needle 10 extends into the needle washing groove 22 to clean the needle body.
In this embodiment, the buffer assembly 20 is provided with a reaction cup holder 21, a needle washing groove 22, a plurality of buffer cups 23, and a plurality of infusion pipelines, the reaction cup holder 21 is used for loading a reaction cup containing a preliminary reaction liquid, at least two buffer cups 23 are used for containing liquids for reaction or cleaning, the suction needle 10 is matched with the buffer assembly 20 to realize suction work of the preliminary reaction liquid or the liquids for reaction or the liquids for cleaning and cleaning work of the suction needle, and further suction efficiency is improved. In addition, a plurality of infusion pipelines are arranged to supplement reaction liquid or cleaning liquid into the buffer cup 23 in time so as to realize continuous suction work; wherein, an infusion pipeline is used for spraying washing liquor into the needle washing groove 22 to realize the cleaning work of the suction needle 10.
In an embodiment, the raw material of the preliminary reaction solution includes a sample to be detected and a part of reaction reagent, the sample to be detected and the part of reaction reagent are mixed and perform incubation reaction, the preliminary reaction solution is obtained after the reaction is completed, and the reaction cup containing the preliminary reaction solution is loaded into the reaction cup holder 21. The reaction liquid supplied in the buffer cup 23 is a luminescent substrate. The aspiration structure body 101 is aspirated with a liquid in the step of luminescence reaction of immunoassay.
In one embodiment, as shown in fig. 1 and 4, the buffer assembly 20 further includes a base 25; the reaction cup holder 21, the needle washing groove 22 and the plurality of buffer cups 23 are mounted on the base 25. Specifically, the base 25 is provided to facilitate assembling the reaction cup holder 21, the needle washing groove 22, the buffer cups 23, and the infusion pipelines.
Further, the base 25 is provided with a plurality of card slots 251; the buffer cup 23 is clamped in the clamping groove 251 so as to be convenient for assembling and disassembling the buffer cup 23.
In an embodiment, the number of the buffer cups 23 is three, including a first buffer cup 231, a second buffer cup 232, and a third buffer cup 233; the number of the liquid conveying pipelines is three, including a first liquid conveying pipeline 241, a second liquid conveying pipeline 242, and a third liquid conveying pipeline 243. The first infusion pipeline 241 is communicated with the first buffer cup 231 and used for conveying cleaning liquid into the first buffer cup 231; the second infusion pipeline 242 is communicated with the second buffer cup 232 and used for conveying reaction liquid into the second buffer cup 232; the third liquid supply line 243 is connected to the needle washing tank 22 for supplying the washing liquid for washing the aspiration needle into the needle washing tank 22. The third buffer cup 233 is used for manually adding a cleaning liquid, and for example, when the suction structure body 101 is maintained or when an unusual cleaning liquid needs to be added in a specific case, the third buffer cup 233 is manually filled with the corresponding cleaning liquid. Furthermore, the third buffer cup 233 is fastened to the base 25, and when the cleaning liquid is manually added, the third buffer cup 233 is first detached from the base 25, and then the cleaning liquid is manually injected, and then the third buffer cup 233 is fastened to the base 25, which is convenient for operation.
In one embodiment, the aspiration needle 10 can perform three-dimensional movement to be positioned with the reaction cup holder 21 or the needle washing slot 22 or several buffer cups 23 at different positions on the buffer assembly 20 to perform aspiration or cleaning work of the aspiration needle.
In another embodiment, as shown in fig. 1 to 3, the method further includes:
a rotating mechanism 30 connected to the buffer assembly 20;
an elevating mechanism 40 connected to the aspiration needle 10;
wherein, the rotating mechanism 30 drives the buffer assembly 20 to rotate horizontally, so that the reaction cup holder 21 or the needle washing groove 22 or the buffer cup 23 is located in the working area of the aspiration needle 10; the elevation mechanism 40 drives the aspiration needle 10 downward to be inserted into a cup or a groove located at a work area. Specifically, the rotating mechanism 30 rotates to switch the region of the buffer member 20 opposed to the position of the aspiration needle 10. When the suction needle 10 sucks the preliminary reaction liquid, the rotation mechanism 30 rotates to make the reaction cup holder 21 correspond to the suction needle 10 in position, so that the suction needle 10 can be inserted into the reaction cup mounted on the reaction cup holder 21 by being driven by the elevation mechanism 40. When the aspiration needle 10 needs to be cleaned, the rotation mechanism 30 is rotated so that the needle wash groove 22 corresponds to the position of the aspiration needle 10. When the aspiration needle 10 aspirates the liquid for washing or the liquid for reaction, the rotation mechanism 30 rotates so that the buffer cup 23 corresponds to the position of the aspiration needle 10. The rotating mechanism 30 is matched with the lifting mechanism 40, so that the positioning work between different parts of the suction needle 10 and the cache assembly 20 is realized, the operation is simple and convenient, the mechanism structure is simple and small, the suction needle 10 moves in the vertical direction, the cache assembly 20 moves in the horizontal direction, and the space required by the movement is saved. In addition, when the rotating mechanism 30 rotates, the plurality of infusion pipelines of the buffer memory assembly 20 keep moving synchronously, so as to prevent the infusion pipelines from being pulled and damaged.
Further, as shown in fig. 4 and 5, the region right below the aspiration needle 10 is the aspiration region or the cleaning region of the aspiration needle 10 for alignment and positioning, and during the operation, the rotation mechanism 30 rotates the corresponding portion on the buffer assembly 20 into the aspiration region or the cleaning region. Wherein, the suction area and the cleaning area are at the same position for distinguishing different working modes of the suction needle 10. When the sucking needle 10 sucks, the reaction cup seat 21 or a buffer cup 23 is positioned in the sucking area; when the aspiration needle 10 is cleaned, the cleaning bath 22 is located in the cleaning area.
Further, as shown in fig. 1 and fig. 2, the device further includes a fixing seat 50, and the rotating mechanism 30 is mounted on the fixing seat 50; the rotating mechanism 30 is a synchronous belt transmission mechanism, one end of a driven wheel rotating shaft 311 is rotatably connected to the fixed seat 50, and the other end is coaxially connected to the buffer assembly 20. Specifically, the fixing seat 50 is configured to provide a supporting force for the buffer assembly 20 and the rotating mechanism 30. The synchronous belt transmission mechanism has large transmission force and stable operation. The rotating mechanism 30 includes a driven wheel 31, a synchronous belt 32, a driving wheel 33, and a second motor 34, the second motor 34 is fixed on the fixing base 50, the driving wheel 33 is coaxially connected to an output end of the second motor 34, the driven wheel 31 rotates under the driving of the synchronous belt 32, and then the buffer module 20 is driven to rotate.
Further, as shown in fig. 1, one end of the driven wheel rotating shaft 311 is rotatably connected to the fixed seat 50 through a bearing 70.
In an embodiment, as shown in fig. 1 and fig. 2, a baffle 61 is sleeved on the periphery of the driven wheel rotating shaft 311, and a correlation type photoelectric sensor 62 is arranged on the fixed seat 50; the baffle 61 and the opposite type photoelectric sensor 62 are matched to position a rotation stop point of the buffer assembly 20, so that accurate positioning is realized.
Further, the baffle 61 is of an annular structure and is provided with a plurality of notches 611; the notches 611 correspond to the positions of the reaction cup holder 21, the needle washing groove 22, and the buffer cup 23, respectively. Specifically, the baffle 61 is simple in structure and easy to assemble. Through the design of arranging a plurality of notches 611 on the baffle 61 and the position of each notch 611, the position detection of each structure on the buffer assembly 20 is realized by matching with the correlation type photoelectric sensor 62. For example, when the needle washing slot 22 rotates to a position right below the suction needle 10, the notch 611 corresponding to the position of the needle washing slot 22 rotates to the position of the correlation type photoelectric sensor 62, the correlation type photoelectric sensor 62 receives the optical signal and sends a stop signal to the second motor 34, the rotation mechanism 30 stops operating, the buffer assembly 20 stops rotating, so that the needle washing slot 22 stops right below the suction needle 10, and the suction needle 10 moves downward to extend into the needle washing slot 22. The positioning principle of the reaction cup seat 21 and the buffer cup 23 is the same as that of the needle washing groove 22, and the description thereof is omitted.
In one embodiment, as shown in fig. 3 and 4, the lifting mechanism 40 includes a fixing plate 41, a first motor 42, and a screw 43 and a sleeve 44; the screw rod 43 is coaxially connected to the output shaft of the first motor 42; the fixing plate 41 is fixed to the sleeve 44 and the aspiration needle 10 respectively;
the first motor 42 drives the screw rod 43 to rotate, so that the sleeve 44 drives the suction needle 10 to move along the screw rod 43. Specifically, a plurality of balls are arranged between the screw rod 43 and the sleeve 44, the screw rod 43 and the sleeve 44 are matched to form a transmission structure, so as to convert the rotation driving force of the first motor 42 into a linear driving force, the sleeve 44 moves up and down along the screw rod 43, and the fixing plate 41 drives the suction needle 10 to realize lifting movement.
Further, the lifting mechanism 40 further includes a guide post 45, and two ends of the guide post are respectively fixed on the surface of the installation object through a connection plate 80; the fixing plate 41 is sleeved on the guide post 45 and is in sliding fit with the guide post 45. When the sleeve 44 drives the fixing plate 41 to move up and down, the fixing plate 41 slides up and down along the guide post 45, and the guide post 45 plays a role of supporting the fixing plate 41.
Further, when the sleeve 44 or the fixing plate 41 moves upward to abut against the connecting plate 80 located at the upper portion of the guide pillar 45, the suction needle 10 is located at the home position, and the rotation of the buffer assembly 20 is not affected by the suction needle 10; when the sleeve 44 or the fixing plate 41 moves down to abut against the connecting plate 80 located at the lower portion of the guide post 45, the suction needle 10 is located at the working position (suction area or washing area). I.e. the guide post 45, can also be used to define the stroke length of the aspiration needle 10, in order to facilitate an electrically controlled design.
In one embodiment, two through holes are formed at the bottom of the needle washing groove 22 for respectively connecting a liquid delivery pipeline and a liquid discharge pipeline. Specifically, the third infusion pipeline 243 is connected to the bottom of the needle washing groove 22 to spray the washing solution upwards from the bottom of the needle washing groove 22, so as to prolong the time of the washing solution remaining on the outer peripheral surface of the suction needle 10, improve the cleaning force and save the amount of the washing solution. One end of the liquid discharge pipeline is communicated with the waste liquid collecting container so as to discharge the waste liquid in the needle washing groove 22 in time.
Example 2
The present invention provides a liquid supply device, as shown in fig. 1 to 5, including a supply device body 100, the supply device body 100 including:
a liquid take-up structure for immunoassay as described above;
a delivery line 102, one end of which is communicated with the suction needle 10 and the other end of which is communicated with the measuring cell 200;
and a plunger pump 103 for generating negative pressure so that the aspiration needle 10 aspirates liquid and then introduces the liquid into the measurement cell through the first conveying pipeline 102.
In this embodiment, the suction structure body 101, the transfer line 102, and the plunger pump 103 are coupled, a preliminary reaction liquid is supplied to the measuring cell 200 through the cuvette mounted on the cuvette holder 21, a remaining required reaction liquid is continuously supplied to the measuring cell through the at least one buffer 23, and liquid suction and liquid transfer to the measuring cell 200 are performed simultaneously, thereby efficiently supplying the liquid.
Further, the conveying pipeline 102 is a light shielding structure. Specifically, because immunoassay needs to be performed based on weak luminescence of the luminescent substrate, the luminescence reaction needs to be performed in a dark room environment when proceeding, so as to improve the luminescence detection accuracy. The measuring cell 200 is a light-shielding structure to provide a dark room environment for the luminescence reaction. The luminous substrate is supplied through the buffer cup 23, the preliminary reaction liquid is supplied through the reaction cup on the reaction cup seat 21, the luminous substrate and the preliminary reaction liquid are respectively put into the measuring pool 200 through the conveying pipeline 102, the light shielding structure of the measuring pool 200 is matched when the feeding device body 100 realizes the high-efficiency putting of each substance in reaction, so that the light shielding environment required by the luminous reaction is formed, the structure is simple, and the liquid supply is high-efficiency.
In one embodiment, plunger pump 103 is disposed on effluent line 300 in communication with measurement cell 200.
In one embodiment, the supply device body 100 is provided with a housing 104, and the suction structure body 101 is mounted to the housing 104. Specifically, the rotating mechanism 30 and the fixing seat 50 are fixed to the housing 104; a partition 1041 is arranged inside the shell 104 to divide the inner space of the shell 104 into an upper cavity and a lower cavity; the buffer memory assembly 20 is positioned in the casing 104 and below the partition 1041, and the first motor 42 is installed on the upper surface of the partition 1041; two connection plates 80 are fixed to the inner side wall of the housing 104. The partition plate 1041 is provided with a mounting hole 10411, the upper end of the suction needle 10 is mounted in the mounting hole 10411 through a seal ring, and the conveying pipeline 102 is connected to the mounting hole 10411 in a penetrating manner to be communicated with the suction needle 10.
In one embodiment, the number of the suction structure bodies 101 is at least two to realize the liquid supply work by different measuring cells.
The working process of the suction structure body 101 is as follows:
s1, sucking the primary reaction liquid
The rotating mechanism 30 drives the reaction cup seat 21 to rotate to a suction area below the suction needle 10, the lifting mechanism 40 drives the suction needle 10 to extend downwards into a reaction cup loaded on the reaction cup seat 21, and after the preliminary reaction liquid is sucked, the suction needle 10 moves upwards to return to the original position;
s2, cleaning the suction needle
The rotating mechanism 30 drives the needle washing groove 23 to rotate to a cleaning area below the suction needle 10, the lifting mechanism 40 drives the suction needle 10 to extend downwards into the needle washing groove 23, a liquid path pipeline communicated with the needle washing groove 23 sprays cleaning liquid to clean the suction needle 10, and after cleaning is finished, the suction needle 10 moves upwards to return to the original position;
s3, aspirating reaction liquid
The rotating mechanism 30 drives the buffer cup 23 containing the reaction liquid to rotate to the suction area below the suction needle 10, the lifting mechanism 40 drives the suction needle 10 to extend downwards into the buffer cup 23, and after the suction of the reaction liquid is finished, the suction needle 10 moves upwards to return to the original position;
s4, sucking the cleaning liquid
The rotating mechanism 30 drives the buffer cup 23 containing the cleaning liquid to rotate to the suction area below the suction needle 10, the lifting mechanism 40 drives the suction needle 10 to extend downwards into the buffer cup 23, and after the cleaning liquid is sucked, the suction needle 10 moves upwards to return to the original position;
s5, aspirating reaction liquid
The step is the same as step S3.
For step S5, after the single test in the measurement cell 200 is completed, the absorption structure body 101 absorbs the reaction liquid and injects the reaction liquid into the measurement cell 200, so as to wash the measurement cell 200, wash the cleaning liquid and discharge the cleaning liquid out of the measurement cell 200, and keep a certain amount of reaction liquid in the measurement cell 200 for the next test. In one embodiment, the reaction liquid is a luminescent substrate.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner; those skilled in the art can readily practice the invention as shown and described in the drawings and detailed description herein; however, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the scope of the invention as defined by the appended claims; meanwhile, any changes, modifications, and evolutions of the equivalent changes of the above embodiments according to the actual techniques of the present invention are still within the protection scope of the technical solution of the present invention.
Claims (10)
1. A liquid take-up structure for immunoassay, comprising:
a suction needle (10) for sucking liquid;
the buffer component (20) comprises a reaction cup seat (21), a needle washing groove (22), a plurality of buffer cups (23) and a plurality of infusion pipelines;
wherein, the reaction cup seat (21) is used for loading a reaction cup containing primary reaction liquid; the needle washing groove (22) is communicated with the infusion pipeline and is used for introducing washing liquor into the needle washing groove (22) to clean the suction needle (10); at least two buffer cups (23) are respectively communicated with one infusion pipeline and are used for introducing and buffering reaction or cleaning liquid;
during suction, the suction needle (10) extends into the reaction cup or the buffer cup (23) to suck liquid; when cleaning, the suction needle (10) extends into the needle washing groove (22) to clean the needle body.
2. The liquid aspiration structure for immunoassay according to claim 1, wherein the buffer assembly (20) further comprises a base (25); the reaction cup seat (21), the needle washing groove (22) and the buffer cups (23) are arranged on the base (25).
3. The liquid aspiration structure for immunoassay according to claim 2, wherein the base (25) is provided with a plurality of card slots (251); the buffer cup (23) is clamped in the clamping groove (251).
4. The liquid take-up structure for immunoassay according to any one of claims 1 to 3, further comprising:
a rotation mechanism (30) connected to the buffer assembly (20);
a lifting mechanism (40) connected to the suction needle (10);
wherein, a rotating mechanism (30) drives the buffer assembly (20) to rotate horizontally, so that the reaction cup seat (21) or the needle washing groove (22) or the buffer cup (23) is positioned in the working area of the suction needle (10); the lifting mechanism (40) drives the suction needle (10) downwards to be inserted into a cup or a groove in a working area.
5. The liquid sucking structure for immunodetection according to claim 4, further comprising a fixing base (50), wherein the rotating mechanism (30) is mounted to the fixing base (50); the rotating mechanism (30) is a synchronous belt transmission mechanism, one end of a driven wheel rotating shaft (311) is rotatably connected to the fixed seat (50), and the other end of the driven wheel rotating shaft is coaxially connected to the buffer assembly (20).
6. The liquid suction structure for the immunodetection as claimed in claim 5, wherein a baffle (61) is sleeved on the periphery of the driven wheel rotating shaft (311), and a correlation type photoelectric sensor (62) is arranged on the fixed seat (50); the baffle (61) and the correlation type photoelectric sensor (62) are matched to position a rotation stop point of the buffer assembly (20).
7. The fluid aspirating structure for immunoassay according to claim 6, wherein said baffle (61) is a ring-shaped structure provided with a plurality of notches (611); the notches (611) correspond to the positions of the reaction cup seat (21) or the needle washing groove (22) or the buffer cup (23) respectively.
8. The liquid sucking structure for immunodetection according to claim 4, wherein the lifting mechanism (40) comprises a fixing plate (41), a first motor (42) and a matched screw rod (43) and sleeve (44); the screw rod (43) is coaxially connected to an output shaft of the first motor (42); the fixing plate (41) is respectively fixed on the sleeve (44) and the suction needle (10);
the first motor (42) drives the screw rod (43) to rotate, so that the sleeve (44) drives the suction needle (10) to move along the screw rod (43).
9. The fluid sucking structure for immunoassay according to claim 1, wherein the bottom of the needle washing tank (22) is provided with two through holes for respectively connecting a fluid delivery pipeline and a fluid discharge pipeline.
10. A liquid supply device, comprising:
a liquid take-up structure for immunoassay according to any one of claims 1 to 9;
a conveying pipeline (102), one end of which is communicated with the suction needle (10), and the other end of which is communicated with the measuring pool;
a plunger pump (103) for forming negative pressure, so that the suction needle (10) sucks liquid and then leads the liquid into the measuring pool through the conveying pipeline (102).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111669153.1A CN114236161A (en) | 2021-12-31 | 2021-12-31 | Liquid suction structure and liquid supply device for immunoassay |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111669153.1A CN114236161A (en) | 2021-12-31 | 2021-12-31 | Liquid suction structure and liquid supply device for immunoassay |
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| CN114236161A true CN114236161A (en) | 2022-03-25 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114965888A (en) * | 2022-05-18 | 2022-08-30 | 深圳无疆生命科学有限公司 | Magnetic separation cleaning device and sample analyzer |
| CN115446032A (en) * | 2022-09-15 | 2022-12-09 | 长沙海柯生物科技有限公司 | Liquid adding cleaning structure and liquid adding cleaning method for liquid sucking needle of immunoassay analyzer |
-
2021
- 2021-12-31 CN CN202111669153.1A patent/CN114236161A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114965888A (en) * | 2022-05-18 | 2022-08-30 | 深圳无疆生命科学有限公司 | Magnetic separation cleaning device and sample analyzer |
| CN114965888B (en) * | 2022-05-18 | 2024-03-01 | 深圳无疆生命科学有限公司 | Magnetic separation cleaning device and sample analyzer |
| CN115446032A (en) * | 2022-09-15 | 2022-12-09 | 长沙海柯生物科技有限公司 | Liquid adding cleaning structure and liquid adding cleaning method for liquid sucking needle of immunoassay analyzer |
| CN115446032B (en) * | 2022-09-15 | 2023-11-10 | 长沙海柯生物科技有限公司 | Liquid adding and cleaning structure and liquid adding and cleaning method for liquid suction needle of immunity analyzer |
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Address after: 211300 No. 3, ancient Tan Road, Gaochun District Economic Development Zone, Nanjing, Jiangsu Applicant after: Nanjing Guoke Precision Medical Technology Co.,Ltd. Address before: 210000 No.3 Gutan Avenue, economic development zone, Gaochun District, Nanjing City, Jiangsu Province Applicant before: NANJING GUOKE MEDICAL TECHNOLOGY DEVELOPMENT Co.,Ltd. |
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Effective date of registration: 20240318 Address after: 211300 No. 3, ancient Tan Road, Gaochun District Economic Development Zone, Nanjing, Jiangsu Applicant after: Nanjing Guoke Precision Medical Technology Co.,Ltd. Country or region after: China Applicant after: Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Address before: 211300 No. 3, ancient Tan Road, Gaochun District Economic Development Zone, Nanjing, Jiangsu Applicant before: Nanjing Guoke Precision Medical Technology Co.,Ltd. Country or region before: China |