CN114657121A - LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的应用 - Google Patents

LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的应用 Download PDF

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CN114657121A
CN114657121A CN202210290086.0A CN202210290086A CN114657121A CN 114657121 A CN114657121 A CN 114657121A CN 202210290086 A CN202210290086 A CN 202210290086A CN 114657121 A CN114657121 A CN 114657121A
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王现伟
张淑红
徐新慧
孙永琨
姚景柯
白晓源
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Abstract

本发明公开了LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的应用，同时本发明设计了用于检测BMSCs中LOX1基因表达量的试剂盒。本发明首次证明了LOX1基因在骨髓间充质干细胞中的作用，且证明LOX1基因在流体剪切力作用下能够促进骨髓间充质干细胞的成骨分化，因此，LOX1基因可以用于制备流体剪切力作用下骨髓间充质干细胞成骨分化的促进剂，来加速骨髓间充质干细胞的成骨分化，从而克服细胞移植来源少的问题。

Description

LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的 应用
技术领域
本发明属于BMSCs成骨分化促进剂技术领域，具体涉及LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的应用。
背景技术
骨缺损是创伤骨科关注的一大问题，骨缺损治疗目前首选的方法是自体骨或异体骨移植，自体骨移植取材有限、取骨有创，且有并发症的出现，其疗效仍不满意。因此，运用组织工程技术在体外成骨后移植回体内是一种新的治疗方式。骨髓间充质干细胞（BMSCs）是一种具有前景的骨再生种子细胞，是骨对环境刺激响应的主要效应细胞。干细胞受多因素、多条信号通路的调控。如何高效的促进种子细胞骨向分化是急需解决的问题。研究BMSCs成骨分化的机制对于临床骨组织再生治疗具有重要意义。
发明内容
本发明的目的是提供了LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的应用，其中LOX1(olr1)是蛋白编码基因，本发明通过实验证实在流体剪切力（4dyns/cm2，2h/d，连续3d）作用下，LOX1基因的表达增加，且可以促进BMSCs成骨分化现象，并与成骨分化的marker基因的表达呈正相关；同时本发明实验设计了LOX1基因（大鼠源Gene ID:293070）表达量检测的试剂盒。
本发明为实现上述目的采用如下技术，LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的应用。
进一步限定，所述流体剪切力的条件为4dyns/cm2，2h/d，连续3d。
进一步限定，用于检测BMSCs中LOX1基因表达量的试剂盒，其特征在于包含特异性扩增LOX1基因的引物；
引物中正向引物序列为：
5'-GCTATCCTTTCTTGGGTGTAAAAC-3'；
引物中反向引物序列为：
5'-TTGCTTCTGGTCTTTGTCTCTG-3'。
本发明与现有技术相比具有以下优点和有益效果：本发明首次证明了LOX1基因在骨髓间充质干细胞中的作用，且证明LOX1基因在流体剪切力作用下能够有效促进骨髓间充质干细胞的成骨分化，因此，LOX1基因可以用于制备流体剪切力作用下骨髓间充质干细胞成骨分化的促进剂，进而来加速骨髓间充质干细胞的成骨分化，从而克服细胞移植来源少的问题。
附图说明
图1是在流体剪切力（F）作用下，BMSCs中LOX1基因表达增加；
图2是在剪切力刺激下，LOX1在表达差异基因中的蛋白互作网络；
图3是慢病毒干扰低表达LOX1的干扰效率；
图4是LOX1基因能够促进BMSCs细胞的成骨分化，增加ALP活性，并与成骨分化标志性基因表达成正相关；
图5是剪切力作用下LOX1基因促进BMSCs细胞的成骨分化现象。
具体实施方式
结合附图详细描述本发明的技术方案。
实施例1
本实施例用于说明在流体剪切力（F）作用下，BMSCs中LOX1基因表达增加，并富集于血液循环系统、细胞连接和活性氧代谢等信号系统。
A、流体剪切力刺激下，成骨诱导的BMSCs中LOX1表达增加
利用体外剪切力装置，将原代培养的大鼠骨髓间充质干细胞（BMSCs）成骨诱导，然后施加一定参数的剪切力刺激（4dyns/cm2, 2h/d, 连续3d），经RNA测序鉴定LOX1基因的表达。结果可见，与对照组（C）相比较，剪切力组（F）的LOX1基因表达增加，如图1中A所示，p < 0.05。
B、Real-time PCR 验证LOX1表达增加
分别取对照组细胞和剪切力刺激组细胞进行qPCR验证，结果如图1中B所示，F组中LOX1表达增加，与测序结果趋势一致，****p <0.0001。
C、KEGG富集LOX1的信号通路
利用R软件包，对测序结果中的LOX1基因进行KEGG富集分析，ggplot2包用于可视化。如图1中C所示，LOX1基因富集于血液循环系统、细胞连接和活性氧代谢等信号系统。本实验结果为研究其促进成骨分化的分子机制提供线索。
实施例2
本实施例用于说明在剪切力刺激下，LOX1在表达差异基因中的蛋白互作网络。
利用R软件包筛选了测序结果中对照组和剪切力刺激组的差异基因，利用string网站进行蛋白互作网络PPI的构建，并进行相关聚类分析。Cytoscape软件进行可视化作图，cytohubba包进行核心基因的筛选。如图2所示，lox1基因为核心基因的TOP30，并通过蛋白聚类分析预测可能与F3等基因具有分子调控关系。为研究LOX1促进成骨分化的分子机制提供理论基础。
实施例3
本实施例用于验证慢病毒干扰低表达LOX1的干扰效率。
图3为BMSCs中慢病毒干扰低表达LOX1的干扰效率。（图3中A）分别验证三个siRNA片段的干扰效率，取干扰效果最佳的片段进行后续的慢病毒质粒构建；（图3中B）构建慢病毒干扰质粒并进行慢病毒干扰，低表达LOX1基因；（图3中C）慢病毒干扰LOX1的效率鉴定，与对照组相比，干扰效率显著，p <0.05。
实施例4
本实施例用于验证LOX1基因促进BMSCs细胞的成骨分化现象。
A、在对照组（C）、剪切力组（F）和剪切力+LOX1干扰组（F-V-LOX1）中进行qPCR验证成骨分化marker基因ALP的表达。如图4中A所示，流体剪切力刺激增加了ALP基因的表达，而低表达LOX1基因后，ALP基因表达减少；
B、qPCR验证成骨分化marker基因Runx2的表达，低表达LOX1基因后，Runx2基因表达减少；
C、分别于C、F和F-V-LOX1组检测ALP活性，图4中C所示低表达LOX1基因后，ALP活力降低。
图4为LOX1基因可以促进BMSCs细胞的成骨分化，且与成骨分化的marker基因ALP和Runx2的表达呈正相关。
实施例5
本实施例用于说明LOX1基因促进BMSCs细胞的成骨分化现象。
A、利用ALP染色检测试剂盒，检测对照组、剪切力组和剪切力+低表达LOX1基因组中ALP的含量。图5中A所示，低表达LOX1基因减弱了ALP的染色强度；
B、茜素红（ARS）染色对照组、剪切力组和剪切力+低表达LOX1基因组。图5中B所示，低表达LOX1基因减弱了ARS染色程度。
图5为LOX1基因可增加BMSCs的成骨分化。
表1 荧光定量RT-PCR引物序列
Figure DEST_PATH_IMAGE001
以上显示和描述了本发明的基本原理，主要特征和优点，在不脱离本发明精神和范围的前提下，本发明还有各种变化和改进，这些变化和改进都落入要求保护的本发明的范围。
SEQUENCE LISTING
<110> 新乡医学院
<120> LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的应用
<130> 2022
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<213> 人工序列（artificial sequence）
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gctatccttt cttgggtgta aaac 24
<210> 2
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<212> DNA
<213> 人工序列（artificial sequence）
<400> 2
ttgcttctgg tctttgtctc tg 22
序列表
<110> 新乡医学院
<120> LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的应用
<130> 2022
<160> 2
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<213> 人工序列(artificial sequence)
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<212> DNA
<213> 人工序列(artificial sequence)
<400> 2
ttgcttctgg tctttgtctc tg 22

Claims (3)

1.LOX1基因作为流体剪切力作用下BMSCs成骨分化促进剂的应用。
2.根据权利要求1所述的应用，其特征在于：所述流体剪切力的条件为4dyns/cm2，2h/d，连续3d。
3.用于检测BMSCs中LOX1基因表达量的试剂盒，其特征在于包含特异性扩增LOX1基因的引物：
引物中正向引物序列为：
5'-GCTATCCTTTCTTGGGTGTAAAAC-3'；
引物中反向引物序列为：
5'-TTGCTTCTGGTCTTTGTCTCTG-3'。
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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060252045A1 (en) * 2003-06-06 2006-11-09 Moitreyee Chatterjee-Kishore Methods and materials for identifying agents which modulate bone remodeling and agents identified thereby
US20080267984A1 (en) * 2007-02-23 2008-10-30 Baylor Research Institute Activation of Human Antigen-Presenting Cells Through Dendritic Cell Lectin-Like Oxidized LDL Receptor-1 (LOX-1)
CN101426536A (zh) * 2006-01-20 2009-05-06 妇女儿童健康研究院公司 治疗、预防和诊断骨病变的方法
US20100291080A1 (en) * 2007-05-31 2010-11-18 Lee Randall J Compositions and methods for tissue repair
CN103889433A (zh) * 2011-08-10 2014-06-25 德普伊新特斯产品有限责任公司 使用脐带组织来源的细胞治疗外周血管疾病
CN104388384A (zh) * 2014-10-29 2015-03-04 河南省华隆生物技术有限公司 氧化型低密度脂蛋白在诱导骨髓间充质干细胞向心肌样细胞分化的应用
KR20180045921A (ko) * 2016-10-25 2018-05-08 강원대학교산학협력단 인간 치조골 유래 중간엽 줄기세포의 증식 및 골형성 분화 촉진 방법 및 그 조성물

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060252045A1 (en) * 2003-06-06 2006-11-09 Moitreyee Chatterjee-Kishore Methods and materials for identifying agents which modulate bone remodeling and agents identified thereby
CN101426536A (zh) * 2006-01-20 2009-05-06 妇女儿童健康研究院公司 治疗、预防和诊断骨病变的方法
US20100330085A1 (en) * 2006-01-20 2010-12-30 Women's & Children's Health Research Institute Inc. Method of treatment, prophylaxis and diagnosis of pathologies of the bone
US20080267984A1 (en) * 2007-02-23 2008-10-30 Baylor Research Institute Activation of Human Antigen-Presenting Cells Through Dendritic Cell Lectin-Like Oxidized LDL Receptor-1 (LOX-1)
CN101668772A (zh) * 2007-02-23 2010-03-10 贝勒研究院 通过树突细胞凝集素样氧化型ldl受体-1(lox-1)激活人抗原呈递细胞
US20100291080A1 (en) * 2007-05-31 2010-11-18 Lee Randall J Compositions and methods for tissue repair
CN103889433A (zh) * 2011-08-10 2014-06-25 德普伊新特斯产品有限责任公司 使用脐带组织来源的细胞治疗外周血管疾病
CN104388384A (zh) * 2014-10-29 2015-03-04 河南省华隆生物技术有限公司 氧化型低密度脂蛋白在诱导骨髓间充质干细胞向心肌样细胞分化的应用
KR20180045921A (ko) * 2016-10-25 2018-05-08 강원대학교산학협력단 인간 치조골 유래 중간엽 줄기세포의 증식 및 골형성 분화 촉진 방법 및 그 조성물

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
FENXI ZHANG 等: ""Lectin-like oxidized LDL receptor-1 expresses in mouse bone marrow-derived mesenchymal stem cells and stimulates their proliferation"", 《EXPERIMENTAL CELL RESEARCH》, vol. 319, pages 1056 *
JI YOON LEE 等: ""Fluid shear stress regulates the expression of Lectin-like oxidized low density lipoprotein receptor-1 via KLF2-AP-1 pathway depending on its intensity and pattern in endothelial cells"", 《ATHEROSCLEROSIS》, vol. 270, pages 76 - 88, XP085354552, DOI: 10.1016/j.atherosclerosis.2018.01.038 *
KIMIKO OHGI 等: ""Toll-like receptor 2 activation primes and upregulates osteoclastogenesis via lox-1"", 《LIPIDS IN HEALTH AND DISEASE》, vol. 17, pages 132 - 140 *
SHAHAB ALIZADEH 等: ""Vitamin D3 Induces Gene Expression of Ox-LDL Scavenger Receptors in Streptozotocin-Induced Diabetic Rat Aortas: New Insight into the Role of Vitamin D in Diabetic Atherosclerosis"", 《REPORTS OF BIOCHEMISTRY & MOLECULAR BIOLOGY》, vol. 6, no. 2, pages 172 *
曹盛楠 等: ""生物力学作用对骨髓间充质干细胞增殖 和成骨分化影响研究进展"", 《山东科学》, vol. 34, no. 2, pages 65 - 74 *

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