CN114656292A - Composite microbial agent for producing liquid bio-organic fertilizer and preparation method thereof - Google Patents
Composite microbial agent for producing liquid bio-organic fertilizer and preparation method thereof Download PDFInfo
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- CN114656292A CN114656292A CN202210366163.6A CN202210366163A CN114656292A CN 114656292 A CN114656292 A CN 114656292A CN 202210366163 A CN202210366163 A CN 202210366163A CN 114656292 A CN114656292 A CN 114656292A
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- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 39
- 239000007788 liquid Substances 0.000 title claims abstract description 36
- 239000002131 composite material Substances 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 230000000813 microbial effect Effects 0.000 title claims description 7
- 239000003795 chemical substances by application Substances 0.000 title claims description 4
- 244000144972 livestock Species 0.000 claims abstract description 39
- 244000144977 poultry Species 0.000 claims abstract description 37
- 210000002700 urine Anatomy 0.000 claims abstract description 33
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 31
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 18
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 16
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 14
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 13
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 13
- 241000186605 Lactobacillus paracasei Species 0.000 claims abstract description 13
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 13
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 13
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims abstract description 13
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 13
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 13
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 9
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 9
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 9
- 239000011574 phosphorus Substances 0.000 claims abstract description 9
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 9
- 239000011591 potassium Substances 0.000 claims abstract description 9
- 241000235070 Saccharomyces Species 0.000 claims abstract description 4
- 241000894006 Bacteria Species 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 14
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 14
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 7
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 7
- 229940099596 manganese sulfate Drugs 0.000 claims description 7
- 239000011702 manganese sulphate Substances 0.000 claims description 7
- 235000007079 manganese sulphate Nutrition 0.000 claims description 7
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 7
- 235000013379 molasses Nutrition 0.000 claims description 7
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 7
- 239000001393 triammonium citrate Substances 0.000 claims description 7
- 235000011046 triammonium citrate Nutrition 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 4
- 210000003608 fece Anatomy 0.000 abstract description 14
- 239000003337 fertilizer Substances 0.000 abstract description 13
- 239000002689 soil Substances 0.000 abstract description 11
- 238000000855 fermentation Methods 0.000 abstract description 10
- 230000004151 fermentation Effects 0.000 abstract description 10
- 239000010871 livestock manure Substances 0.000 abstract description 7
- 239000006041 probiotic Substances 0.000 abstract description 6
- 235000018291 probiotics Nutrition 0.000 abstract description 6
- 239000003513 alkali Substances 0.000 abstract description 4
- 230000006872 improvement Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 2
- 238000009335 monocropping Methods 0.000 abstract description 2
- 238000005067 remediation Methods 0.000 abstract description 2
- 235000013311 vegetables Nutrition 0.000 abstract description 2
- 235000013594 poultry meat Nutrition 0.000 description 33
- 238000000926 separation method Methods 0.000 description 8
- 238000003973 irrigation Methods 0.000 description 6
- 230000002262 irrigation Effects 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 3
- 244000061458 Solanum melongena Species 0.000 description 3
- 235000002597 Solanum melongena Nutrition 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000271566 Aves Species 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a composite microbial inoculum for producing liquid bio-organic fertilizer and a preparation method thereof, belonging to the technical field of microorganism, comprising the following steps: lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus acidophilus, Lactobacillus rhamnosus, Saccharomyces longosporangium, Saccharomyces cerevisiae, and Bacillus belgii. The invention provides a simple and easy-to-operate method for producing a biological organic fertilizer from livestock and poultry manure in a farm, the fertilizer efficiency is obvious, and the liquid organic fertilizer rich in organic matters, nitrogen, phosphorus and potassium and various crop probiotics is finally produced by fermentation, so that the problem of environmental pollution caused by a large amount of urine is solved, waste can be changed into valuable, bacterial manure capable of increasing the soil organic matters and the probiotics required by soil and plants is produced, and the biological organic fertilizer can be used for landscaping, field continuous cropping soil remediation, vegetable greenhouse economic crops, saline-alkali soil improvement and the like.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a composite microbial inoculum for producing a liquid bio-organic fertilizer and a preparation method thereof.
Background
In recent years, with the rapid development of livestock and poultry breeding in our country, the scale and intensification degree of livestock and poultry breeding is continuously improved, a large amount of excrement and sewage generated by livestock and poultry breeding become important pollution sources, and especially around large cities, the environment around the cities is worsened due to the pollution of the excrement and urine of the livestock and poultry, and surface water and underground water are seriously polluted. At present, livestock and poultry breeding pollution becomes an important source of agricultural non-point source pollution, and is not neglected or avoidable, and the problem of breaking comprehensive utilization of excrement is urgent and must be looked at. The harmless treatment and recycling comprehensive utilization of livestock and poultry manure are key points, the problem of environmental pollution caused by the manure of a farm is solved, waste is changed into valuable, the manure is converted into organic fertilizer, meanwhile, the yield and income of agricultural production are increased, and the economic sustainable development is realized.
In large-scale beasts and birds breeding enterprise, it is at present generally to adopt dry wet separation method to carry out preliminary treatment to beasts and birds excrement and sewage, and dry part mainly carries out microbial fermentation and produces the fertilizer, has eliminated poisonous harmful substance wherein through fermentation treatment, and is rich in a large amount of active substance profitable to the crop, includes: the organic acid fertilizer has the advantages of providing comprehensive nutrition for crops, increasing and updating soil organic matters, promoting microbial propagation, improving physical and chemical properties and biological activity of soil, and being capable of serving as a main nutrient for green food production. In addition, liquid dung dirt, urine and the like obtained after the dung dirt of the farm is subjected to dry-wet separation can be converted into liquid bio-organic fertilizers through microbial fermentation, and water and fertilizer integration is realized.
The water and fertilizer integration technology is used for comprehensively regulating and managing farmland water and nutrients according to crop demands, promoting fertilizer by water and regulating water by fertilizer to realize water and fertilizer coupling, has important popularization and application values for water conservation and efficient utilization of farmland fertilizers, particularly in general water-deficient areas in the northern China, and can be used as water-soluble fertilizers for landscaping, hillside forest lands, saline-alkali land improvement, field rotation irrigation and water-saving drip irrigation.
The traditional fecal sewage treatment is mainly characterized in that after dry-wet separation, solid part is subjected to compost solid state fermentation, while liquid part has no particularly suitable treatment method, and currently, the harmless treatment such as denitrification, desalination, dephosphorization and the like is mainly carried out through an aerobic aeration tank, an anaerobic sedimentation tank and biogas production, and finally generated reclaimed water and biogas slurry are recycled, but the treatment cost is high, and secondary pollution is easily caused.
Disclosure of Invention
In view of the above, the invention aims to provide a complex microbial inoculum and a complex microbial inoculum for producing a liquid-state bio-organic fertilizer and a preparation method thereof.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a composite microbial inoculum for producing liquid bio-organic fertilizer, which comprises the following components: lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus acidophilus, Lactobacillus rhamnosus, Saccharomyces longosporangium, Saccharomyces cerevisiae, and Bacillus belgii.
Preferably, the number of viable bacteria of lactobacillus plantarum in each gram of the composite microbial inoculum is 180-220 hundred million, the number of viable bacteria of lactobacillus paracasei is 180-220 hundred million, the number of viable bacteria of lactobacillus acidophilus is 180-220 hundred million, the number of viable bacteria of lactobacillus rhamnosus is 180-220 hundred million, the number of viable bacteria of lodoromyces longosporus is 180-220 hundred million, the number of viable bacteria of saccharomyces cerevisiae is 1800-2200 hundred million, and the number of viable bacteria of bacillus belgii is 45000-55000 hundred million.
The invention also provides a method for preparing the liquid bio-organic fertilizer by using the composite microbial inoculum, which comprises the following steps: and mixing the composite microbial agent with the livestock and poultry urine, and standing and fermenting for more than 3 days at the temperature of 20-30 ℃ to obtain the liquid biological organic fertilizer.
Preferably, the volume ratio of the mass of the composite microbial inoculum to the livestock urine is 100-120 g:1m3。
Preferably, each cubic meter of livestock and poultry urine also contains 2-4L of molasses, 0.3-0.8 kg of dipotassium hydrogen phosphate, 0.03-0.08 kg of magnesium sulfate, 0.1-0.3 kg of triammonium citrate, 0.05-0.15 kg of calcium carbonate and 0.03-0.08 kg of manganese sulfate.
Preferably, the complex microbial inoculum is preferably dissolved in water and then is inoculated in the urine of livestock and poultry by spraying.
Preferably, the mass ratio of the composite microbial inoculum to water is 100-120: 10.
The invention also provides a liquid biological organic fertilizer prepared by the preparation method based on the technical scheme, wherein the liquid biological organic fertilizer contains more than 40% of organic matters, more than 10% of nitrogen, phosphorus and potassium and more than 10 hundred million cfu/mL of effective viable bacteria.
The compound microbial inoculum for producing the liquid bio-organic fertilizer provided by the invention is based on various environment-friendly plant probiotics, namely strains with plant growth promoting and disease resisting characteristics, carries out reasonable nutrition proportioning on the pig urine after dry-wet separation, and fully mixes and ferments to realize concentrated harmless treatment of the pig urine and feces, thereby removing peculiar smell substances in the pig urine and producing the liquid organic bacterial fertilizer rich in organic matters, nitrogen, phosphorus and potassium and various crop probiotics through fermentation.
The invention has the beneficial effects that:
the invention provides a simple and easy-to-operate method for producing a biological organic fertilizer from livestock and poultry manure in a farm, the fertilizer efficiency is obvious, and the liquid organic fertilizer rich in organic matters, nitrogen, phosphorus and potassium and various crop probiotics is finally produced by fermentation, so that the problem of environmental pollution caused by a large amount of urine is solved, waste can be changed into valuable, bacterial manure capable of increasing the soil organic matters and the probiotics required by soil and plants is produced, and the biological organic fertilizer can be used for landscaping, field continuous cropping soil remediation, vegetable greenhouse economic crops, saline-alkali soil improvement and the like.
Detailed Description
The invention provides a composite microbial inoculum for producing a liquid bio-organic fertilizer, which comprises the following components: lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus acidophilus, Lactobacillus rhamnosus, Saccharomyces longosporangium, Saccharomyces cerevisiae, and Bacillus belgii.
In the present invention, the lactobacillus plantarum is preferably lactobacillus plantarum ACCC 11016; the lactobacillus paracasei is preferably lactobacillus paracasei CGMCC 1.9089; the lactobacillus acidophilus is preferably lactobacillus acidophilus ACCC 19940; the lactobacillus rhamnosus is preferably lactobacillus rhamnosus ACCC 10534; the described long spore lode yeast is preferably long spore lode yeast ACCC 20322; the saccharomyces cerevisiae is preferably saccharomyces cerevisiae ACCC 20065; the Bacillus belgii is preferably Bacillus belgii ACCC 02747.
In the invention, the composite strain has the following functions: the Bacillus belgii is used as a decomposed strain, can quickly absorb soluble and easily decomposed organic matters and has a main effect on fermentation and temperature rise. The yeast can be decomposed and converted into ammonia gas and hydrogen sulfide by using nitrogen-containing and sulfur-containing organic matters, and the lactobacillus can generate organic acid by using a substrate, so that main peculiar smell gases such as ammonia gas, hydrogen sulfide, mercaptan, methyl mercaptan and the like can be effectively decomposed.
In the invention, the number of viable bacteria of lactobacillus plantarum in each gram of the composite microbial inoculum is preferably 180-220 hundred million, the number of viable bacteria of lactobacillus paracasei is preferably 180-220 hundred million, the number of viable bacteria of lactobacillus acidophilus is preferably 180-220 hundred million, the number of viable bacteria of lactobacillus rhamnosus is preferably 180-220 hundred million, the number of viable bacteria of lodoromyces longisporum is preferably 180-220 hundred million, the number of viable bacteria of saccharomyces cerevisiae is preferably 1800-2200 hundred million, and the number of viable bacteria of bacillus belgii is preferably 45000-55000 hundred million; the number of viable bacteria of the lactobacillus plantarum is preferably 200 hundred million, the number of viable bacteria of the lactobacillus paracasei is preferably 200 hundred million, the number of viable bacteria of the lactobacillus acidophilus is preferably 200 hundred million, the number of viable bacteria of the lactobacillus rhamnosus is preferably 200 hundred million, the number of viable bacteria of the lodoromyces longosporus is preferably 200 hundred million, the number of viable bacteria of the saccharomyces cerevisiae is preferably 2000 hundred million, and the number of viable bacteria of the bacillus beiLeisi is preferably 50000 hundred million. The invention also provides a method for preparing the liquid bio-organic fertilizer by using the composite microbial inoculum, which comprises the following steps: and mixing the composite microbial agent with the livestock and poultry urine, and standing and fermenting for more than 5 days at the temperature of 20-30 ℃ to obtain the liquid biological organic fertilizer.
In the invention, the ratio of the mass of the composite microbial inoculum to the volume of the livestock and poultry urine is excellentIs selected to be 100-120 g:1m3(ii) a More preferably 100g:1m3. In the invention, per cubic meter of livestock and poultry urine also preferably contains 2-4L of molasses, 0.3-0.8 kg of dipotassium hydrogen phosphate, 0.03-0.08 kg of magnesium sulfate, 0.1-0.3 kg of triammonium citrate, 0.05-0.15 kg of calcium carbonate and 0.03-0.08 kg of manganese sulfate; more preferably, 3L of molasses, 0.5kg of dipotassium hydrogen phosphate, 0.05kg of magnesium sulfate, 0.2kg of triammonium citrate, 0.1kg of calcium carbonate and 0.05kg of manganese sulfate are contained. The reagents are used as substrates, and the addition of the reagents is beneficial to the growth and utilization of fermentation strains, the fermentation process of the organic fertilizer is accelerated, the growth of the strains is promoted, and the fermentation efficiency is improved.
In the invention, the livestock and poultry urine is preferably livestock and poultry urine obtained after the dry-wet separation of livestock and poultry feces. In the present invention, the livestock and poultry preferably include pigs and/or chickens.
In the invention, the complex microbial inoculum is preferably dissolved in water and then is inoculated in the urine of livestock and poultry by spraying. In the invention, the mass ratio of the complex microbial inoculum to water is preferably 100-120: 10, and more preferably 100: 10.
The invention provides a liquid biological organic fertilizer prepared by the preparation method based on the technical scheme, which contains more than 40% of organic matters, more than 10% of nitrogen, phosphorus and potassium and more than 10 hundred million cfu/mL of effective viable bacteria. In the invention, the liquid bio-organic fertilizer has a light acid fragrance.
In the invention, the application method of the liquid bio-organic fertilizer preferably comprises the following steps: preferably, the liquid bio-organic fertilizer is diluted by 20-100 times by using water and then is subjected to drip irrigation or furrow application on plants, such as landscape plants, landscaping fertilization, field crop fertilization or improvement on saline-alkali soil.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Dissolving 110g of the composite microbial inoculum in 10L of water, spraying and inoculating the mixture in 1 cubic meter of livestock and poultry urine, and standing and fermenting for 5 days at 30 ℃ to obtain the liquid biological organic fertilizer.
And (4) carrying out dry-wet separation on the livestock and poultry feces to obtain the livestock and poultry urine.
The compound microbial inoculum added in each ton of livestock and poultry urine is as follows: lactobacillus plantarum 10g (20 hundred million/gram), lactobacillus paracasei 10g (20 hundred million/gram), lactobacillus acidophilus 10g (20 hundred million/gram) and lactobacillus rhamnosus 10g (20 hundred million/gram), lodoromyces longosporus 10g (200 hundred million/gram), saccharomyces cerevisiae 10g (200 hundred million/gram), bacillus belgii 50 g (1000 hundred million/gram).
2L of molasses, 0.3kg of dipotassium phosphate, 0.03kg of magnesium sulfate, 0.1kg of triammonium citrate, 0.05kg of calcium carbonate and 0.03kg of manganese sulfate are also added into per cubic meter of livestock and poultry urine.
The prepared liquid bio-organic fertilizer contains 26% of organic matters, 8% of nitrogen, phosphorus and potassium and 5 hundred million cfu/mL of effective viable bacteria.
After being diluted by 50 times, the liquid biological organic fertilizer is applied to eggplants by drip irrigation, and as a result, the crop yield can be improved by 8%.
Example 2
110g of the composite microbial inoculum is dissolved in 10L of water, sprayed and inoculated in 1 cubic meter of livestock and poultry urine, and then kept stand and fermented for 5 days at 30 ℃ to obtain the liquid biological organic fertilizer.
And (4) carrying out dry-wet separation on the livestock and poultry feces to obtain the livestock and poultry urine.
The compound microbial inoculum added in each ton of livestock and poultry urine is as follows: lactobacillus plantarum 10g (18 hundred million/gram), lactobacillus paracasei 10g (18 hundred million/gram), lactobacillus acidophilus 10g (18 hundred million/gram) and lactobacillus rhamnosus 10g (18 hundred million/gram), lodoromyces longosporus 10g (180 hundred million/gram), saccharomyces cerevisiae 10g (180 hundred million/gram), bacillus belgii 50 g (900 hundred million/gram).
Each cubic meter of livestock and poultry urine is also added with 3L of molasses, 0.5kg of dipotassium phosphate, 0.05kg of magnesium sulfate, 0.2kg of triammonium citrate, 0.1kg of calcium carbonate and 0.05kg of manganese sulfate.
The prepared liquid bio-organic fertilizer contains 42% of organic matters, 10% of nitrogen, phosphorus and potassium and 10 hundred million cfu/mL of effective viable bacteria.
After being diluted by 50 times, the liquid biological organic fertilizer is applied to eggplants by drip irrigation, and the yield of crops can be improved by 15 percent.
Example 3
110g of the composite microbial inoculum is dissolved in 10L of water, sprayed and inoculated in 1 cubic meter of livestock and poultry urine, and then kept stand and fermented for 5 days at 30 ℃ to obtain the liquid biological organic fertilizer.
And (4) carrying out dry-wet separation on the livestock and poultry feces to obtain the livestock and poultry urine.
The compound microbial inoculum added in each ton of livestock and poultry urine is as follows: 10g (22 hundred million/gram) of Lactobacillus plantarum, 10g (22 hundred million/gram) of Lactobacillus paracasei, 10g (22 hundred million/gram) of Lactobacillus acidophilus and 10g (22 hundred million/gram) of Lactobacillus rhamnosus, 10g (220 hundred million/gram) of Saccharomyces cerevisiae, and 50 g (1100 hundred million/gram) of Bacillus belgii.
4L of molasses, 0.8kg of dipotassium hydrogen phosphate, 0.08kg of magnesium sulfate, 0.3kg of triammonium citrate, 0.15kg of calcium carbonate and 0.08kg of manganese sulfate are also added into per cubic meter of livestock and poultry urine.
The prepared liquid bio-organic fertilizer contains 35% of organic matters, 12% of nitrogen, phosphorus and potassium and 10 hundred million cfu/mL of effective viable bacteria.
After being diluted by 50 times, the liquid biological organic fertilizer is applied to eggplants by drip irrigation, and the yield of crops can be improved by 12 percent.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (8)
1. A composite microbial inoculum for producing liquid bio-organic fertilizer, which is characterized by comprising: lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus acidophilus, Lactobacillus rhamnosus, Saccharomyces longosporangium, Saccharomyces cerevisiae, and Bacillus belgii.
2. The composite microbial inoculum according to claim 1, wherein the number of viable bacteria of lactobacillus plantarum is 180-220 billion per gram of composite microbial inoculum, the number of viable bacteria of lactobacillus paracasei is 180-220 billion, the number of viable bacteria of lactobacillus acidophilus is 180-220 billion, the number of viable bacteria of lactobacillus rhamnosus is 180-220 billion, the number of viable bacteria of lodoromyces longisporum is 180-220 billion, the number of viable bacteria of saccharomyces cerevisiae is 1800-2200 billion, and the number of viable bacteria of bacillus belgii is 45000-55000 billion.
3. A method for preparing a liquid bio-organic fertilizer by using the composite microbial inoculum of claim 1 or 2, which comprises the following steps: and mixing the composite microbial agent with the livestock and poultry urine, and standing and fermenting for more than 5 days at the temperature of 20-30 ℃ to obtain the liquid biological organic fertilizer.
4. The preparation method according to claim 3, wherein the volume ratio of the mass of the complex microbial inoculum to the livestock urine is 100-120 g:1m3。
5. The preparation method of claim 3, wherein the livestock and poultry urine per cubic meter further comprises 2-4L of molasses, 0.3-0.8 kg of dipotassium hydrogen phosphate, 0.03-0.08 kg of magnesium sulfate, 0.1-0.3 kg of triammonium citrate, 0.05-0.15 kg of calcium carbonate and 0.03-0.08 kg of manganese sulfate.
6. The preparation method according to claim 3, wherein the complex microbial inoculum is preferably dissolved in water and then is inoculated in the urine of livestock and poultry by spraying.
7. The preparation method according to claim 6, wherein the mass ratio of the complex microbial inoculum to water is 100-120: 10.
8. The liquid bio-organic fertilizer prepared by the preparation method of any one of claims 3 to 7 contains more than 40% of organic matters, more than 10% of nitrogen, phosphorus and potassium and more than 10 hundred million cfu/mL of effective viable bacteria.
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