CN114651771A - Epinephelus coioides breeding treatment system - Google Patents
Epinephelus coioides breeding treatment system Download PDFInfo
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- 238000011282 treatment Methods 0.000 title claims abstract description 137
- 241000357444 Epinephelus coioides Species 0.000 title claims abstract description 132
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- 238000012545 processing Methods 0.000 claims description 28
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- 241000277275 Oncorhynchus mykiss Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
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- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
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- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 1
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- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
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- JNMRHUJNCSQMMB-UHFFFAOYSA-N sulfathiazole Chemical compound C1=CC(N)=CC=C1S(=O)(=O)NC1=NC=CS1 JNMRHUJNCSQMMB-UHFFFAOYSA-N 0.000 description 1
- 229960001544 sulfathiazole Drugs 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
- A01K63/006—Accessories for aquaria or terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The method creates the epinephelus coioides breeding treatment system capable of preventing and treating the cryptocaryon irritans for the first time, screens and optimizes various effective physical and chemical treatment means, and has an effective resistance effect on the cryptocaryon irritans infecting the epinephelus coioides. The epinephelus coioides cultivation treatment system comprises an acetic acid treatment tank, a hunger treatment area, an ultraviolet light treatment area, a magnetization treatment area, an oxygenation treatment area, an ultrasonic treatment area, an orange green light treatment area and a nutrition strengthening tank which are sequentially arranged, and the cultivation treatment system obtains good effects no matter in a test research stage or a production practice stage.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to a culture treatment system capable of effectively improving the survival rate of epinephelus coioides.
Background
The Epinephelus coioides belongs to the order Perciformes, bass and genus Epinephelus, and is highly favored by the southeast Asia market because of its tender meat, delicious taste and rich nutrition. The Epinephelus coioides has the characteristics of high growth speed, strong environmental adaptability, high survival rate and the like, and has remarkable economic benefit, thereby being deeply welcomed by breeding manufacturers. Nowadays, the culture of Epinephelus coioides is one of the important industries of mariculture in China, the culture yield is over 10 ten thousand tons since 2015, the Epinephelus coioides is a main object of mariculture in coastal provinces in south China, and the culture modes of the Epinephelus coioides mainly comprise cage culture, pond culture and the like. However, with the rapid increase of the culture yield of the Epinephelus coioides, the increase of the culture density, the continuous deterioration of the culture environment and the frequent outbreak of the diseases of the Epinephelus coioides, the development of the mariculture industry in China is seriously puzzled. In recent years, the frequent outbreak of white spot disease of Epinephelus coioides in coastal areas in south China is more and more extensive, becomes a main epidemic disease for the mariculture of Fujian, Zhejiang, Guangdong, Guangxi and Hainan, and causes huge economic loss to the mariculture industry in China.
Cryptocaryon irritans disease is also called as 'marine ichthyophthiriasis', is a main disease of marine cultured fishes, mainly occurs in tropical and subtropical marine culture areas, and reports of cryptocaryon irritans outbreak are reported from time to time in Australia, New Zealand, southern United states, Kowitt, Thailand, Singapore, Malaysia, Taiwan in China, and coastal areas in southern continental. The etiological challenge Cryptocaryon irritans Brown 1951 is a ciliate parasite in the gill and skin cuticle of marine teleosts. The clinical symptoms of the disease are: white spots, mucus increase, dyspnea and anorexia appear on the body surface and gills, and diseased fishes show behavior characteristics of easy fright, fast swimming, reverse water flow swimming and the like. Some of the identifying characteristics of cryptocaryon irritans are described in detail by Lom and Dykova (1992), and the method for determining cryptocaryon irritans disease is: the fish body had continuously rotating elliptic ciliates in the gill, skin or fin line, and white spots were visually observed.
At present, the method for preventing and treating the seawater ichthyophthiriasis mainly comprises four types, the first type is a physical method which mainly comprises heat treatment, ultraviolet ray treatment, ozone treatment and the like, and the methods play a certain role in the process of preventing and treating the seawater ichthyophthiriasis, but have limited effects. Secondly, an ecological method is also explored by foreigners about the ecological prevention and control of cryptocaryon irritans, such as drying treatment, fresh water fish body soaking, alternate culture and the like. And thirdly, chemical prevention and treatment, wherein a lot of researches on soaking medicines are carried out in chemical prevention and treatment measures, such as sodium hypochlorite, benzalkonium chloride, copper sulfate, formalin, potassium permanganate, sulfathiazole and the like, but the chemical treatment measures also have certain defects, for example, some medicines have side effects on fish bodies, and some curative effects are not very definite. Fourthly, an immunological method is adopted, in 1942, Duff feeds the oncorhynchus mykiss with inactivated aeromonas salmonicida for the first time, so that the host fish obtains stronger immune protection capability, and a milestone for research and application of fish vaccines is established; it was found by roche et al (2007) that either intraperitoneal injection or epitopic infection stimulates cryptocaryon larvae, a protective immune response to the parasite in Epinephelus coioides was elicited and antibodies were detected in serum and skin culture supernatants of immunized fish; however, the current vaccine research on the seawater ichthyophthiriasis is still in the laboratory stage, and no stable and reliable vaccine is available on the market. Therefore, in the process of culturing the epinephelus coioides, a culture treatment system capable of effectively resisting the cryptocaryon irritans is urgently needed.
Disclosure of Invention
The invention aims to solve the problem of difficulty in preventing and treating cryptocaryon irritans in the epinephelus coioides breeding process, and provides the epinephelus coioides breeding treatment system which can be used for sequentially treating the epinephelus coioides in a flow manner, is convenient to operate, is safe and effective, has small side effect on the epinephelus coioides, and has a good effect of preventing and treating the cryptocaryon irritans. The method is not only suitable for the Epinephelus coioides infected with the cryptocaryon irritans or suspected to be infected with the cryptocaryon irritans, but also suitable for the Epinephelus coioides not suffering from diseases so as to improve the disease resistance of the Epinephelus coioides.
The method creates the epinephelus coioides cultivation treatment system capable of preventing and treating the cryptocaryon irritans for the first time, screens and optimizes various effective physical and chemical treatment means, and has an effective resisting effect on the cryptocaryon irritans infecting the epinephelus coioides.
The invention is realized by the following technical scheme:
the utility model provides a garrupa culture treatment system, including acetic acid processing pond, hunger processing area, ultraviolet ray processing area, magnetization processing area, oxygenating processing area, ultrasonic treatment district, orange green light processing area, the nutrition enhancement pond of arranging in proper order, acetic acid processing pond is located the foremost of breed processing system, and the nutrition enhancement pond is located the most terminal of breed processing system, hunger processing area, ultraviolet ray processing area, magnetization processing area, oxygenating processing area, ultrasonic treatment district, orange green light processing area are closed breed the room, and it can communicate each other to breed the room above, all separates each other through the gate, all sets up a breed indoor and all set up a breed oneThe driving net, the driving motor and the control switch, wherein the control switch can turn on the driving motor so as to drive the driving net to drive the epinephelus coioides to the next culture room. The areas of the acetic acid treatment tank, the starvation treatment area, the ultraviolet light treatment area, the magnetization treatment area, the oxygenation treatment area, the ultrasonic treatment area, the orange green light treatment area and the nutrition strengthening tank are all 8m2About, the water depth is about 0.8m, wherein, the mass concentration in the acetic acid treatment tank is 60-80ml/m3The acetic acid water solution is characterized in that a positive electrode plate and a negative electrode plate are arranged in an acetic acid treatment pool, the voltage for electrifying the positive electrode plate and the negative electrode plate is 1-1.5V, the current is 10-12mA, an ultraviolet germicidal lamp is arranged in an ultraviolet light treatment area, the generated wavelength is 280-300 nm, a magnetization switch is arranged in the magnetization treatment area, a variable electromagnetic field is introduced into the magnetization treatment area after the magnetization switch is turned on, the introduction time of the electromagnetic field is 1h, the intensity of the electromagnetic field is changed from weak to strong and then becomes weak, the intensity is respectively 230-. An oxygenation pump is arranged in the oxygenation treatment area, and the dissolved oxygen in the oxygenation treatment area reaches more than 11 mg/L. An ultrasonic generator is arranged in the ultrasonic processing area, the power of the generated ultrasonic is 1000W, and the frequency is 28-30 kHz. The orange green light processing area is provided with an orange light lamp, a green light lamp and a switch, after the switch is turned on, the orange light lamp is turned off after being turned on for 15min, the green light lamp is turned off after being turned on for 15min, the wavelength generated by the orange light lamp is 590-600nm, the illumination intensity is 1000-1200 lx, the wavelength generated by the green light lamp is 520-530nm, and the illumination intensity is 800-900 lx.
The use method of the Epinephelus coioides breeding treatment system comprises the following steps: putting the epinephelus coioides into an acetic acid treatment tank, wherein the mass concentration of the epinephelus coioides is 60-80ml/m3Soaking the fish in the acetic acid aqueous solution for 20min, applying weak current, voltage of 1-1.5V, current of 10-12mA, and electrifying for 10min to the negative electrode plate and the positive electrode plate, transferring the epinephelus coioides to a starvation treatment area, opening a gate and a control switch of the starvation treatment area after starvation treatment for about 4 hours, and driving a driving net to drive the epinephelus coioides to the ultraviolet light treatment area by a driving motor; after being treated by ultraviolet light with the wavelength of 280-300 nm for 8min, the ultraviolet light positions are opened successivelyThe gate and the control switch of the treatment area drive the motor to drive the driving net to drive the Epinephelus coioides to the magnetization treatment area; opening the magnetization switch, sequentially introducing electromagnetic fields of 230-, 250-, 750-, and 430-450mT for 20min respectively, then sequentially opening the gate and the control switch of the magnetization processing area, and driving the driving net by the driving motor to drive the Epinephelus coioides to the oxygenation processing area; the dissolved oxygen in the oxygenation treatment area reaches the supersaturation level, the dissolved oxygen reaches more than 11mg/L, after the epinephelus coioides stays in the oxygenation treatment area for 40 min, a gate and a control switch of the oxygenation treatment area are opened in sequence, and a driving motor drives a driving net to drive the epinephelus coioides to the ultrasonic treatment area; after ultrasonic treatment for 10min with the power of 1000W and the frequency of 28-30kHz, opening a gate and a control switch of the ultrasonic treatment area in sequence, and driving a driving net by a driving motor to drive the Epinephelus coioides to the orange green light treatment area; opening a switch in the orange-green light treatment area, opening a gate and a control switch of the orange-green light treatment area after an orange light lamp and a green light lamp are turned on for 15min respectively, and driving a driving net by a driving motor to drive the Epinephelus coioides to a nutrition enhancement pool; temporarily culturing Epinephelus coioides in a nutrition-enriched pool for three days, and feeding for 6 times every day: feeding rotifer brachypomus at 7:00, wherein the density of the rotifer is kept at 10-20/ml; feeding the frozen fresh mixed fish meat paste at a ratio of 10:00, wherein the feeding amount is 5-8% of the weight of the fish; 13:00, feeding shrimp meat, wherein the feeding amount is 10-13% of the weight of the fish; feeding mixed solution of artemia and daphnia at a ratio of 16:00, wherein the feeding density is 80-100/mL; 19:00, feeding expanded feed with the particle size of 2 mm and the protein content of less than 30 percent, wherein the feeding amount is 10-13 percent of the weight of the fish; 22:00, feeding the seawater fish with the crude protein content of more than 50 percent by using a complete formula feed, wherein the feeding amount is 7-9 percent of the weight of the fish; after three days of nutrition enhancement, the epinephelus coioides can be transported out of the culture treatment system for normal culture.
The epinephelus coioides breeding treatment system can be used for not only the epinephelus coioides infected with cryptocaryon irritans or suspected to be infected with cryptocaryon irritans, but also the epinephelus coioides which is not diseased, so as to prevent the infection of the cryptocaryon irritans.
The invention has the advantages and effects that: the method has the advantages that the epinephelus coioides breeding treatment system is adopted for preventing and treating cryptocaryon irritans for the first time, various treatment means are screened and optimized for carrying out flow-process treatment on the epinephelus coioides, the operation is easy, no adverse effect is caused on the epinephelus coioides, and the immunity and the disease resistance to cryptocaryon irritans of the epinephelus coioides can be effectively improved.
The acetic acid aqueous solution is weakly acidic, not only can disinfect the fish body, but also can enhance the activity of the epinephelus coioides, stimulate the epinephelus coioides to get rid of cryptocaryon irritans on gills and skins, and meanwhile, a weak current is introduced into the water body by virtue of the electrode plate, so that electrons in the acetic acid solution flow, further stimulate the epinephelus coioides to get rid of the cryptocaryon irritans, and the disease resistance of the epinephelus coioides is improved.
The starvation treatment can reduce the stress response of the subsequent treatment program to the epinephelus coioides, the final nutrition strengthening can effectively supplement the energy consumption of the epinephelus coioides in the treatment process, different baits are fed in six different time periods in a targeted manner, the nutrition is effectively supplemented, and the immunity and the disease resistance of the epinephelus coioides are improved.
The method has the advantages that the vitality and immunity of the epinephelus coioides can be enhanced through ultraviolet light treatment, magnetization treatment, oxygen supersaturation treatment, ultrasonic treatment and orange green light treatment, the treatment technologies are combined together, and through optimizing various treatment parameters, the method is not only beneficial to improving the vitality and disease resistance of the epinephelus coioides, but also capable of generating stimulation to cryptocaryon irritans to stimulate the cryptocaryon irritans to separate from fish bodies, and the method is beneficial to the epinephelus coioides to get rid of the infection of the cryptocaryon irritans.
The ultrasonic waves and the electromagnetic field have medical biological effects, the ultrasonic waves with certain frequency and the electromagnetic field radiation with certain intensity can enhance the activity of enzymes in fish and shrimp bodies, improve the hatching of fertilized eggs of the fish and shrimp, promote the growth and development of seedlings and the like, after the ultrasonic waves with proper light intensity and the electromagnetic field radiation with proper light intensity can improve the growth speed of the fish and shrimp and enhance the immunologic function of the fish and shrimp, and the ultrasonic waves with high intensity and the electromagnetic field radiation with high intensity can break biological cells and reduce the activity of the enzymes and the like. Ultrasonic waves with different frequencies and electromagnetic field radiation with different intensities can have certain influence on the digestibility of the young fishes and shrimps, which is probably because the low-frequency ultrasonic waves and the low-intensity electromagnetic field radiation can change the conformation of certain digestive enzymes in the bodies of the fishes and shrimps to change the catalytic activity of the digestive enzymes, thereby changing the combination degree of enzyme molecules and substrates to influence the digestion of the young fishes and shrimps. The ultrasonic frequency and the electromagnetic field intensity suitable for the epinephelus coioides are optimized and screened by the ultrasonic treatment area and the magnetization treatment area, so that the growth speed of the epinephelus coioides can be improved, the immune function of the epinephelus coioides is enhanced, and the cryptocaryon irritans can be stimulated to separate from the epinephelus coioides.
The orange light and the green light can influence the enzymatic activity in the fish body to a certain extent, can influence the growth and development of the fish body, the orange green light with proper wavelength and light intensity can improve the growth speed of the Epinephelus coioides and improve the disease resistance of the fish body, and simultaneously the orange light and the green light can also generate bad stimulation to the cryptocaryon irritans, so that the cryptocaryon irritans irregularly moves and falls off from the body surface of the host. The orange-green light processing area optimally screens the orange light wavelength light intensity and the green light wavelength light intensity suitable for the epinephelus coioides, which is not only beneficial to the improvement of the disease resistance of the epinephelus coioides, but also beneficial to the stimulation of cryptocaryon irritans to be separated from the epinephelus coioides.
Detailed Description
EXAMPLE one optimization of acetic acid concentration and electrode plate energization time in an acetic acid treatment tank
Six sets of acetic acid concentrations were set: 20ml/m3、40ml/m3、60ml/m3、80ml/m3、100ml/m3、120ml/m3Separately soaking the epinephelus coioides infected with cryptocaryon irritans for 20min, counting the cryptocaryon irritans on gills of the epinephelus coioides before and after soaking, and observing the stress response of the epinephelus coioides. The test result shows that the concentration is 20ml/m3、40ml/m3、60ml/m3、80ml/m3、100ml/m3、120ml/m3After the fish is soaked in the acetic acid solution, the cryptocaryon irritans at gills of the epinephelus coioides are respectively reduced by 10%, 20%, 34%, 37% and 38%. Epinephelus coioides 20ml/m3、40ml/m3、60ml/m3、80ml/m3Has no stress response to the concentration of (2), and is 100ml/m3、120ml/m3The concentration of (a) shows a stress response. Therefore, the concentration of the acetic acid in the acetic acid treatment pool is selected to be 60-80ml/m3。
The mass concentration is 60-80ml/m3The method comprises the steps of inserting an electrode plate into an acetic acid aqueous solution, electrifying weak current, enabling the voltage to be 1-1.5V and the current to be 10-12mA, setting five test groups and a blank control group, wherein the electrifying time of the five test groups is respectively 5min, 10min, 15min, 20min and 25min, the blank control group is not electrified, putting the Epinephelus coioides with the same amount of infection of the Epinephelus coioides into the six test groups, counting the Epinephelus coioides at the gill part of the Epinephelus coioides before and after treatment, and observing the stress response of the Epinephelus coioides. Test results show that the cryptocaryon irritans at gills of epinephelus coioides are reduced by 15%, 35%, 36%, 37% and 38% respectively by electrifying for 5min, 10min, 15min, 20min and 25 min. The number of cryptocaryon irritans in the blank control group did not change. The Epinephelus coioides is electrified for 5min and 10min without stress reaction, and the Epinephelus coioides is electrified for 15min, 20min and 25min to generate stress reaction. Thus, the power-on time was selected to be 10 min.
EXAMPLE two optimization of UV treatment time
And (3) respectively treating the epinephelus coioides infected with the cryptocaryon irritans for 0min, 4min, 6min, 8min, 10min and 12min by using ultraviolet light with the wavelength of 280-300 nm, counting the cryptocaryon irritans at the gill parts of the epinephelus coioides before and after treatment, and observing the stress response of the epinephelus coioides. Test results show that 0min, 4min, 6min, 8min, 10min and 12min of treatment respectively reduce the cryptocaryon irritans at the gills of the epinephelus coioides by 0%, 5%, 10%, 28%, 29% and 30%. The method has no stress reaction when the Epinephelus coioides is treated for 0min, 4min, 6min and 8min, and the Epinephelus coioides has stress reaction when the power is applied for 10min and 12 min. Therefore, the UV light is selected for 8 min.
EXAMPLE III optimization of the electromagnetic field intensity in the magnetized treatment zone
Four test groups are arranged, the first group of electromagnetic field strength is 230-250mT, the second group of electromagnetic field strength is 730-750mT, the third group of electromagnetic field strength is 430-450mT, the fourth group of electromagnetic field is a variable electromagnetic field which is changed from weak to strong and then becomes weak, and the blank control group is not accessed with the electromagnetic field. Respectively treating the Epinephelus coioides infected with the cryptocaryon irritans for 1h, and respectively introducing electromagnetic fields of 230-750 mT, 730-750mT and 430-450mT for 20min in the fourth group, and respectively counting the cryptocaryon irritans at the gill parts of the Epinephelus coioides before and after treatment. The test result shows that the fourth group of electromagnetic field treatment has the largest influence on cryptocaryon irritans, so that the cryptocaryon irritans at the gill part of the epinephelus coioides is reduced by 39%. The number of cryptocaryon irritans in the blank control group did not change. No obvious stress reaction of the epinephelus coioides of the four test groups occurs.
EXAMPLE four optimization of ultrasonic frequency in sonication zone
Respectively treating Epinephelus coioides infected with cryptocaryon irritans with ultrasonic waves with frequencies of 18-20kHz, 28-30kHz, 38-40kHz, 48-50kHz and 58-60kHz for 10min, respectively counting the cryptocaryon irritans at the branchia of the Epinephelus coioides before and after treatment, and observing the stress response of the Epinephelus coioides. Test results show that ultrasonic treatment with the frequency of 28-30kHz has the largest influence on cryptocaryon irritans, so that the cryptocaryon irritans on gills of Epinephelus coioides is reduced by 45%. No obvious stress reaction occurs in the epinephelus coioides in the five groups of experiments.
EXAMPLE V optimization of treatment time in orange-green treatment zone
Eight test groups are set, the first group is orange light irradiation for 10min, the second group is orange light irradiation for 15min, the third group is orange light irradiation for 20min, the fourth group is green light irradiation for 10min, the fifth group is green light irradiation for 15min, the sixth group is green light irradiation for 20min, the seventh group is orange light irradiation and green light irradiation for 10min, the eighth group is orange light irradiation and green light irradiation for 15min, the blank control group is not irradiation, the orange light wavelength is 590-152 nm, the illumination intensity is 1000-1200 lx, the green light wavelength is 520-530nm, and the illumination intensity is 800-900 lx. Before and after treatment, the cryptocaryon irritans at the gill parts of the epinephelus coioides are counted respectively, and the stress response of the epinephelus coioides is observed. Test results show that the eighth group of treatments had the greatest effect on cryptocaryon irritans, reducing cryptocaryon irritans in gills of Epinephelus coioides by 41%. The number of cryptocaryon irritans in the blank control group did not change. No obvious stress response of the epinephelus coioides of the eight test groups occurs.
EXAMPLE VI optimization of nutrient enrichment
The control group was fed with conventional diet 3 times a day. Two test groups are provided, wherein the first group feeds the bait 6 times a day and feeds conventional baits; the second group was fed 6 times per day: feeding rotifer brachypomus at 7:00, wherein the density of the rotifer is kept at 10-20/ml; 10:00, feeding the frozen fresh trash fish meat paste, wherein the feeding amount is 5-8% of the weight of the fish; 13:00, feeding shrimp meat, wherein the feeding amount is 10-13% of the weight of the fish; feeding mixed solution of artemia and daphnia at a ratio of 16:00, wherein the feeding density is 80-100/mL; 19:00, feeding expanded feed with the particle size of 2 mm and the protein content of less than 30 percent, wherein the feeding amount is 10-13 percent of the weight of the fish; 22:00, and feeding the seawater fish with the crude protein content of more than 50 percent by using a complete formula feed, wherein the feeding amount is 7-9 percent of the weight of the fish. The feeding time is 3 days, and the fed object is the epinephelus coioides which is infected with the cryptocaryon irritans and sequentially subjected to acetic acid treatment, hunger treatment, ultraviolet light treatment, magnetization treatment, oxygenation treatment, ultrasonic treatment and orange green light treatment. And measuring the weight change of the Epinephelus coioides before and after feeding. The test results show that the second group of feeding modes has the greatest influence on the fish body weight, so that the fish body weight is increased by 9 percent. The control group increased by 2% and the first group by 3%. And the vitality of the epinephelus coioides fed by the second group is obviously better than that of the other two groups.
EXAMPLE VII application of Epinephelus coioides cultivation treatment System
Putting the epinephelus coioides infected with cryptocaryon irritans into an acetic acid treatment pool, soaking for 20min by using an acetic acid aqueous solution with the mass concentration of 60-80ml/m 3, then introducing weak current, the voltage of 1-1.5V, the current of 10-12mA and the energization time of 10min to a negative electrode plate and a positive electrode plate, then transferring the epinephelus coioides into a hunger treatment area, after hunger treatment for about 4 hours, successively opening a gate and a control switch of the hunger treatment area, and driving a driving net by a driving motor to drive the epinephelus coioides to an ultraviolet light treatment area; after the treatment for 8min by ultraviolet light of 280-300 nm, opening a gate and a control switch of the ultraviolet light treatment area in sequence, and driving a driving net by a driving motor to drive the epinephelus coioides to a magnetization treatment area; opening the magnetization switch, sequentially introducing electromagnetic fields of 230-, 250-, 750-, and 430-450mT for 20min respectively, then sequentially opening the gate and the control switch of the magnetization processing area, and driving the driving net by the driving motor to drive the Epinephelus coioides to the oxygenation processing area; the dissolved oxygen in the oxygenation treatment area reaches the supersaturation level, the dissolved oxygen reaches more than 11mg/L, after the epinephelus coioides stays in the oxygenation treatment area for 40 min, a gate and a control switch of the oxygenation treatment area are opened in sequence, and a driving motor drives a driving net to drive the epinephelus coioides to the ultrasonic treatment area; after the fish is treated by ultrasonic waves with the power of 1000W and the frequency of 28-30kHz for 10min, a gate and a control switch of an ultrasonic treatment area are opened in sequence, and a driving motor drives a driving net to drive the Epinephelus coioides to a green orange light treatment area; opening a switch in the orange-green light treatment area, opening a gate and a control switch of the orange-green light treatment area after an orange light lamp and a green light lamp are sequentially and respectively lighted for 15min, and driving a driving net to drive the epinephelus coioides to a nutrition strengthening pool by a driving motor; temporarily culturing Epinephelus coioides in a nutrition-enriched pool for three days, and feeding for 6 times every day: feeding rotifer brachypomus at 7:00, wherein the density of the rotifer is kept at 10-20/ml; 10:00, feeding the frozen fresh trash fish meat paste, wherein the feeding amount is 5-8% of the weight of the fish; 13:00, feeding shrimp meat, wherein the feeding amount is 10-13% of the weight of the fish; feeding mixed solution of artemia and daphnia at a ratio of 16:00, wherein the feeding density is 80-100/mL; 19:00, feeding expanded feed with the particle size of 2 mm and the protein content of less than 30 percent, wherein the feeding amount is 10-13 percent of the weight of the fish; 22:00, feeding the seawater fish with the crude protein content of more than 50 percent by using a complete formula feed, wherein the feeding amount is 7-9 percent of the weight of the fish; after three days of nutrition enhancement, the epinephelus coioides is transported out of the culture treatment system, cryptocaryon irritans infection conditions of body surfaces and gills of the epinephelus coioides are detected, and cryptocaryon irritans residue is not detected, namely the cryptocaryon irritans completely fall off.
Comparative examples 1 to 8: comparative examples 1 to 8 compared with example seven, one treatment zone (acetic acid treatment tank, starvation treatment zone, ultraviolet light treatment zone, magnetization treatment zone, oxygenation treatment zone, ultrasonic treatment zone, orange-green light treatment zone, nutrient enrichment tank) was reduced, and other compositions and treatment methods were the same as example seven. Finally, the infection condition of the treated Epinephelus coioides is detected, and a small amount of residual cryptocaryon irritans is detected in comparative examples 1 to 8, namely the cryptocaryon irritans does not completely fall off.
Claims (6)
1. The utility model provides an oblique area grouper breed processing system which characterized in that: the device comprises an acetic acid treatment tank, a hunger treatment area, an ultraviolet light treatment area, a magnetization treatment area, an oxygenation treatment area, an ultrasonic treatment area, an orange green light treatment area and a nutrition strengthening tank which are sequentially arranged, wherein the acetic acid treatment tank is positioned at the foremost end of a culture treatment system, the nutrition strengthening tank is positioned at the rearmost end of the culture treatment system, the hunger treatment area, the ultraviolet light treatment area, the magnetization treatment area, the oxygenation treatment area, the ultrasonic treatment area and the orange green light treatment area are all closed culture chambers, the culture chambers can be mutually communicated and are mutually separated through a gate, a driving net, a driving motor and a control switch are arranged in each culture chamber, and the control switch can open the driving motor to drive the epinephelus coioides to the next culture chamber; the areas of the acetic acid treatment tank, the starvation treatment area, the ultraviolet light treatment area, the magnetization treatment area, the oxygenation treatment area, the ultrasonic treatment area, the orange green light treatment area and the nutrition strengthening tank are all 8m2About, the water depth is about 0.8m, wherein, the mass concentration in the acetic acid treatment tank is 60-80ml/m3The acetic acid water solution is characterized in that a positive electrode plate and a negative electrode plate are arranged in an acetic acid treatment pool, the voltage for electrifying the positive electrode plate and the negative electrode plate is 1-1.5V, the current is 10-12mA, an ultraviolet germicidal lamp is arranged in an ultraviolet light treatment area, the generated wavelength is 280-300 nm, a magnetization switch is arranged in the magnetization treatment area, a variable electromagnetic field is introduced into the magnetization treatment area after the magnetization switch is turned on, the introduction time of the electromagnetic field is 1h, the intensity of the electromagnetic field is changed from weak to strong and then becomes weak, the intensity is respectively 230-.
2. An oxygenation pump is arranged in the oxygenation treatment area, and the dissolved oxygen in the oxygenation treatment area reaches more than 11 mg/L; an ultrasonic generator is arranged in the ultrasonic processing area, the power of the generated ultrasonic is 1000W, and the frequency is 28-30 kHz.
3. The orange green light processing area is provided with an orange light lamp, a green light lamp and a switch, after the switch is turned on, the orange light lamp is turned off after being turned on for 15min, the green light lamp is turned off after being turned on for 15min, the wavelength generated by the orange light lamp is 590-600nm, the illumination intensity is 1000-1200 lx, the wavelength generated by the green light lamp is 520-530nm, and the illumination intensity is 800-900 lx.
4. The use method of the Epinephelus coioides breeding treatment system comprises the following steps: putting the epinephelus coioides into an acetic acid treatment tank, wherein the mass concentration of the epinephelus coioides is 60-80ml/m3Soaking the fish in the acetic acid aqueous solution for 20min, applying weak current, voltage of 1-1.5V, current of 10-12mA, and electrifying for 10min to the negative electrode plate and the positive electrode plate, transferring the epinephelus coioides to a starvation treatment area, opening a gate and a control switch of the starvation treatment area after starvation treatment for about 4 hours, and driving a driving net to drive the epinephelus coioides to the ultraviolet light treatment area by a driving motor; after the treatment for 8min by ultraviolet light of 280-300 nm, opening a gate and a control switch of the ultraviolet light treatment area in sequence, and driving a driving net by a driving motor to drive the epinephelus coioides to a magnetization treatment area; opening the magnetization switch, sequentially introducing electromagnetic fields of 230-, 250-, 750-, and 430-450mT for 20min respectively, then sequentially opening the gate and the control switch of the magnetization processing area, and driving the driving net by the driving motor to drive the Epinephelus coioides to the oxygenation processing area; the dissolved oxygen in the oxygenation treatment area reaches the supersaturation level, the dissolved oxygen reaches more than 11mg/L, after the epinephelus coioides stays in the oxygenation treatment area for 40 min, a gate and a control switch of the oxygenation treatment area are opened in sequence, and a driving motor drives a driving net to drive the epinephelus coioides to the ultrasonic treatment area; after ultrasonic treatment for 10min with the power of 1000W and the frequency of 28-30kHz, opening a gate and a control switch of the ultrasonic treatment area in sequence, and driving a driving net by a driving motor to drive the Epinephelus coioides to the orange green light treatment area; opening a switch in the orange-green light treatment area, opening a gate and a control switch of the orange-green light treatment area after an orange light lamp and a green light lamp are respectively lighted for 15min, and driving a driving net to drive the epinephelus coioides to a nutrition strengthening pool by a driving motor; temporarily culturing Epinephelus coioides in a nutrition-enriched pool for three days, and feeding for 6 times every day: 7:00, feeding fold armThe density of the tail rotifer is kept between 10 and 20 rotifers/ml; 10:00, feeding the frozen fresh trash fish meat paste, wherein the feeding amount is 5-8% of the weight of the fish; 13:00, feeding shrimp meat, wherein the feeding amount is 10-13% of the weight of the fish; feeding mixed solution of artemia and daphnia at a ratio of 16:00, wherein the feeding density is 80-100/mL; 19:00, feeding expanded feed with the particle size of 2 mm and the protein content of less than 30 percent, wherein the feeding amount is 10-13 percent of the weight of the fish; 22:00, feeding the seawater fish with the crude protein content of more than 50 percent by using a complete formula feed, wherein the feeding amount is 7-9 percent of the weight of the fish; after three days of nutrition enhancement, the epinephelus coioides can be transported out of the culture treatment system for normal culture.
5. The Epinephelus coioides cultivation processing system of claim 1, wherein: the epinephelus coioides breeding treatment system is not only suitable for the epinephelus coioides infected with cryptocaryon irritans or suspected to be infected with cryptocaryon irritans, but also suitable for the epinephelus coioides which is not diseased.
6. The Epinephelus coioides cultivation processing system of claim 1, wherein: the concentration of the acetic acid aqueous solution is 80ml/m3。
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