CN107027667A - A kind of hydroxyl radical free radical kills the method and apparatus for stimulating cryptonucleus insect packing - Google Patents

Abstract

A method and device for killing and stimulating Cryptocaryon cyst by hydroxyl radicals, equipped with a water storage tank, a breeding pond, a high-frequency high-voltage power supply, a modular oxygen active fragment generator, an online oxygen active fragment detector, and a Venturi gas-liquid Mixer, liquid-liquid mixer, TRO detector, motor, automatic rotating brush, water pump, valve, gas pipeline and liquid main pipeline; the air inlet of the modular oxygen active debris generator is connected to an external oxygen source, and the modular oxygen The oxygen active fragment gas outlet of the active fragment generator is connected with the online oxygen active fragment detector and the suction port of the Venturi gas-liquid miscible in sequence; The liquid inlet of the miscible is connected, the liquid outlet of the Venturi gas-liquid miscible is connected with the liquid inlet of the liquid-liquid miscible, and the liquid outlet of the liquid-liquid miscible is connected with the TRO detector and the water inlet of the breeding pond in turn; An automatic rotating brush controlled by a motor is arranged in the middle of the culture pond; the water outlet at the bottom of the culture pond is externally connected to seawater.

Description

A method and device for killing and stimulating Cryptocaryon cyst by hydroxyl radicals

technical field

The invention relates to the fields of plasma chemistry, seawater aquaculture technology, marine environmental engineering and the like, in particular to a method and device for killing and stimulating Cryptocaryon cyst by hydroxyl radicals.

Background technique

Cryptocaryon stimuli is a kind of ciliate that causes "white spot disease" in marine fish. The disease is prone to outbreaks in water bodies with high stocking density, poor fluidity and rich organic matter content, and usually parasitizes tropical and subtropical marine fish. The skin, fins, gills, etc. of the fish will cause damage and dysfunction of fish organs, cause abnormal activity, epithelial hyperplasia, breathing difficulties and mechanical damage of diseased fish, cause a large number of fish deaths, and cause great harm to the mariculture industry.

The life cycle of Cryptocaryon stimuli includes four stages: "trophoblast", "cyst precursor", "cyst" and "larvae". Trophozoites are parasitic growth and development in the epithelial tissue of diseased fish after the larvae are infected with the diseased fish. The growth period is 3-7 days; after being mature, they will detach from the host diseased fish in the early morning and develop into cyst precursors; after 8-12 hours The cyst precursor sheds its cilia and adheres to the underwater support to form a hard shell cyst; the cyst undergoes a series of asymmetric binary fissions for 3 to 20 days to form progeny larvae, and each cyst can release 200 to 400 Larvae, larvae swim in water and infect host fish to form trophozoites for the next life cycle (Dan Xueming. Studies on the passage, preservation and prevention of Cryptocaryon irritans[D]. Jinan University, 2006).

With the development of our country's economy, people's demand for seafood is gradually increasing. Only relying on traditional marine fishing is far from meeting the market demand, and the marine fish farming industry has developed rapidly. Cryptocaryon stimuli causes great harm to the mariculture industry due to its fast transmission speed, high mortality rate, and difficulty in prevention and control. However, Cryptocaryon stimuli in the stage of cyst life history have several layers of cyst walls, and there are dense substances between the layers. The outer layer of the cyst is difficult to break, and it is difficult for ordinary drugs to enter the inside of the cyst. Traditional methods cannot effectively kill the cyst. . The key to the prevention and control of Cryptocaryonia irritatus is to kill the cysts of Cryptocaryonia stimuli and prevent it from developing and releasing more larvae and reinfecting farmed fish. How to kill Cryptocaryon irritant cysts efficiently, energy-saving and environment-friendly has attracted more and more attention. At present, some physical and chemical methods are often used in mariculture production to kill Cryptocaryon stimuli cysts. The physical methods mainly include low-pressure ultraviolet irradiation, immersion of cysts in fresh water, and rotation breeding, etc., but the effect is not obvious, and often cannot effectively kill the cysts. Chemical methods include splashing copper sulfate, formalin, potassium permanganate solution, etc. into the diseased breeding ponds. Although these methods have a certain killing effect on cysts, they cannot be effectively and completely killed. Cysts can still continue to develop and release larvae to infect host fish, and long-term overdose will also cause problems such as drug residues, toxicity to fish, enhanced drug resistance of cysts, and environmental pollution. Li Anxing et al. use the method of laying pads to remove cysts at the bottom of the culture pond to control and treat Cryptocaryoniasis irritant (seeing Chinese patent CN105532534A). How to efficiently kill Cryptocaryon stimuli cysts in an energy-efficient and environmentally friendly way has attracted more and more attention.

Contents of the invention

The purpose of the present invention is to solve the problems such as poor cyst killing effect and serious environmental pollution in the existing seawater aquaculture, and to provide a solution that can kill Cryptocaryon stimuli cysts and prevent cysts. A method and device for killing and stimulating Cryptocaryon cysts by hydroxyl free radicals that develop and release more larvae to repeatedly infect farmed fish.

A device for killing and stimulating Cryptocaryon cyst by hydroxyl free radicals is equipped with a water storage tank, a breeding pond, a high-frequency high-voltage power supply, a modular oxygen active fragment generator, an online oxygen active fragment detector, and a Venturi gas-liquid miscible device , liquid-liquid miscible device, TRO detector, motor, automatic rotating brush, water pump, valve, gas pipeline and liquid main pipeline; The oxygen active debris gas outlet of the debris generator is connected with the online oxygen active debris detector and the suction port of the Venturi gas-liquid miscible in sequence; The liquid inlet of the liquid miscible is connected, the liquid outlet of the Venturi gas-liquid miscible is connected with the liquid inlet of the liquid-liquid miscible, and the liquid outlet of the liquid-liquid miscible is connected with the TRO detector and the water inlet of the breeding pond in turn ; The center of the cultivation pond is provided with an automatic rotating brush controlled by a motor; the water outlet at the bottom of the cultivation pond is externally connected to seawater.

An oxygen valve and a gas flow meter can be arranged sequentially between the oxygen source and the modular oxygen active debris generator; oxygen-containing active debris can be arranged sequentially between the online oxygen active debris detector and the Venturi gas-liquid miscible device. Fragment gas valve, oxygen-containing active fragment gas flow meter; a main pipeline liquid valve may be provided between the water storage tank and the water pump, and a liquid flow meter may be provided between the water pump and the Venturi gas-liquid miscible device; the culture pond An external discharge liquid valve can be provided between the external seawater; a sampling valve for the main water inlet pipeline can be provided between the TRO detector and the cultivation pond, and an external discharge sampling valve can be provided between the cultivation pond and the external seawater.

A method for killing and stimulating Cryptocaryon cyst by hydroxyl radicals, comprising the following steps:

1) From July to November every summer, when the water temperature is 25-30°C to stimulate the outbreak of cryptocystosis, if the cultured fish eat less food, rub the pool wall or bottom with their bodies, the mucus on the body surface will increase and there will be obvious white spots, And some fry died; use a microscope to observe the white spots on the surface of the dead fish, and find that they are black round or pear-shaped, and you can confirm the occurrence of cryptocystosis; at 10:00 in the morning, a large number of cysts from the disease After the fish falls off, all the diseased fish in the diseased breeding pond are fished out to other clean breeding ponds without disease, and the water level in the diseased breeding pond is lowered. At this time, there are a large number of irritating Cryptocaryon cysts attached to the bottom of the diseased breeding pond;

2) Turn on the switch of the modular oxygen active fragment generator, open the oxygen valve to feed oxygen, start the high-frequency high-voltage power supply, so that the oxygen passing through the modular oxygen active fragment generator is rapidly ionized and dissociated into gaseous oxygen active fragments; open the oxygen-containing active fragment Fragment valve, the gaseous oxygen active fragments enter the Venturi gas-liquid miscible device after the concentration of the gaseous oxygen active fragments is detected by the online oxygen active fragment concentration detector;

3) Open the water pump and the liquid valve of the main pipeline, and the clean seawater stored in the water storage tank is injected into the Venturi gas-liquid miscible through the pipeline through the water pump; the gaseous oxygen active fragments and seawater entering the Venturi gas-liquid mixing solvent in the step 2) are Fully miscible in the Venturi gas-liquid miscible and liquid-liquid miscible to produce a high-concentration hydroxyl radical solution, which enters the culture pond after passing through the TRO detector through the pipeline;

4) After the hydroxyl radical solution is injected into the culture pond, soak the bottom of the culture pond to stimulate Cryptocaryon cysts for 0.5 to 2.0 hours, and the hydroxyl radical solution oxidizes the weak area of the cyst wall to make holes appear and enter the inside of the cyst to form the basic cells. Structural substances and functional substances undergo irreversible reactions, the irreversible reactions include oxidative decomposition of amino acids, DNA strand breaks, etc., so that the cysts die and cannot further develop and release larvae, and the remaining hydroxyl radicals are decomposed into O ₂ and H ₂ O; in the middle of the breeding pool It is equipped with an automatic rotating brush controlled by a motor. After starting, it will automatically rotate to clean the cysts in the pond; after rotating for 10 minutes, open the liquid discharge valve to discharge the dead cysts in the breeding pond with the sea water.

In step 1), the culture pond is a cylinder-like open cement pond with a radius of 2m, a depth of 1.5m, and a total volume of about 20m ³ . There is a water inlet above the side of the breeding pond, and the bottom of the pond is a horizontal and smooth bottom. In the center of the pond, there is an automatic rotating brush controlled by a motor. The rotating axis is a stainless steel tube. The brush plate is a smooth brush plate made of polypropylene with a length of 3.8m and a width of 0.3m, and is clothed with bristles made of nylon wire with a length of 0.15m; after the motor is started, the brush can rotate along the central axis stainless steel tube at a speed of 10r/min , There is a drainage port at the bottom of the side of the pool wall.

In step 2), the power of the high-frequency high-voltage power supply is 150-300W, the oxygen intake rate is 1.0-5.0L/min, and the oxygen is ionized and dissociated after being bombarded by high-energy electrons to form gaseous oxygen active fragments. The active fragments of gaseous oxygen mainly include particles such as O ₂ ⁺ , O( ¹ D), O ₂ (a ¹ g), O ₂ ^- , O( ³ P) and O ₃ , and the concentration can reach 50-300 mg/L; The pipeline is polytetrafluoroethylene tube.

In step 3), the flow rate of the seawater is 10-50 L/min, the concentration of the hydroxyl radical solution is regulated by the water temperature, the oxygen active fragment concentration, the equilibrium partial pressure of the oxygen-containing active fragment gas and the gas-liquid volume ratio, and the total oxidant The concentration of TRO is 2-30mg/L; the storage tank is a square closed cement tank with a total volume of about 20m ³ , and the stored seawater is clean seawater that has been pretreated by sand filtration.

In step 4), the stimulated Cryptocaryon cysts are immersed in the hydroxyl radical solution for 0.5 to 2.0 hours, and after confirming the death of the cysts through microscopic examination, etc., start the automatic rotating brush switch, and after rotating for 10 minutes, culture The dead cysts in the pool are discharged along with the sea water, and the liquid pipeline used can be made of anti-corrosion treated stainless steel pipes, and the treated culture pool can continue to breed fish.

The present invention uses atmospheric pressure ionization discharge to ionize and dissociate oxygen into high-concentration oxygen active fragments in an extremely narrow discharge gap, and then efficiently and rapidly generates a hydroxyl radical solution within 1 second through gas-liquid miscibility and liquid-liquid miscibility. The hydroxyl radical solution acts on the weak area of the cyst wall to make holes appear and enter the interior of the cyst. The substances and functional substances that make up the basic structure of the cell undergo irreversible reactions such as amino acid oxidation and decomposition, DNA strand breaks, etc., so that the cyst dies and prevents its development and release. More larvae will repeatedly infect farmed fish, so as to achieve the effect of controlling cryptocystosis. Hydroxyl free solution can quickly kill residual harmful bacteria and other microorganisms in the culture pond while killing the cysts of Cryptocaryon stimuli, preventing the occurrence of diseases and insect pests.

In the device for killing and stimulating Cryptocaryon cyst by hydroxyl radicals of the present invention, the oxidant concentration is regulated by water temperature, concentration of oxygen active fragments, equilibrium partial pressure and gas-liquid volume ratio of oxygen-containing active fragment gas; The number of attached cysts determines the concentration and soaking time of the hydroxyl radical solution. The hydroxyl radical solution with a concentration of 5.0-10.0mg/L can effectively kill the cysts of the stimulated Cryptocaryonia within 0.5-2.0 hours, preventing them from continuing to develop form larvae.

The present invention uses hydroxyl free radicals (OH) generated based on atmospheric pressure ionization discharge to kill and stimulate Cryptocaryon cysts. Hydroxyl radical (·OH), ·OH is a green and strong oxidant, which has the characteristics of fast, efficient, and can react with biological macromolecules, and generate non-polluting H ₂ O and O ₂ after the reaction, without residual chemicals. The method and device for killing Cryptocaryon stimuli cysts based on hydroxyl radicals provide a new solution for seawater aquaculture to prevent and control Cryptocaryon stimuli.

The technical effects and advantages of the present invention are: ①The oxygen is passed into the miniaturized non-equilibrium plasma source with an extremely narrow discharge gap of 0.1 mm and submicron Al ₂ O ₃ paste into a thin dielectric layer, realizing atmospheric pressure ionization Discharge generates high-concentration oxygen active particles; adopt modular array plasma integrated sources and combine them arbitrarily to obtain adjustable and large-scale production of oxygen active particles, and cooperate with water jet cavitation gas-liquid miscibility technology to obtain high-concentration hydroxyl radicals Solution, the total oxidant TRO concentration is 2.0-30.0mg/L; ②Cryptonia irritatus cysts adhering to the bottom of the culture pond are soaked in the hydroxyl radical solution with a concentration of 5.0-10.0mg/L for 0.5-2.0h, which can Realize the complete killing of Cryptocaryon stimulatory cysts and prevent them from developing and releasing more larvae to repeatedly infect farmed fish; ③The hydroxyl free solution can disinfect and sterilize the breeding pool while killing Cryptocaryon stimulatory cysts , prevent pests and diseases; ④Use the automatic rotating brush to completely remove the dead Cryptocaryon stimuli cysts adhering to the bottom of the culture pond, and the dead cysts are directly discharged with the remaining seawater in the pond; ⑤The device described in the invention is simple to implement and low in operating costs Low, no drug residue, no toxicity to fish, and no pollution to the environment while efficiently killing Cryptocaryon irritant cysts, providing a healthy, environmentally friendly and efficient device for killing Cryptocaryon irritant cysts in seawater aquaculture.

Description of drawings

FIG. 1 is a schematic diagram of the structure and composition of an embodiment of the device for killing and stimulating Cryptocaryon cysts by hydroxyl radicals of the present invention.

Fig. 2 is the change of capsule morphology before and after the killing of hydroxyl radicals according to the embodiment of the present invention. In Fig. 2, A and C are photos of cyst morphology in the control group; B and D are photos of cyst morphology when TRO=5.27mg/L.

detailed description

The present invention will be further described in conjunction with the accompanying drawings. However, the scope of protection of the invention is not limited to the examples described.

Referring to Fig. 1, the device for quickly killing and stimulating Cryptocaryon cysts by hydroxyl radicals is provided with a water storage tank 1, a Venturi gas-liquid miscible device 5, a high-frequency high-voltage power supply 6, and a modular oxygen active debris generator 7 , online oxygen active debris detector 8, liquid-liquid miscible device 9, TRO detector 10, motor 11, automatic rotating brush 12 and culture pond 13.

The water outlet of the water storage tank 1 is connected to the water inlet of the water pump 31, and the first valve 21 is arranged between the water storage tank 1 and the water pump 31; The first flow meter 41 is set between the Venturi gas-liquid miscibles 5; the air inlet of the modular oxygen active debris generator 7 is connected to an external oxygen source, and the second flow meter is sequentially set between the modular oxygen active debris generator 7 and the oxygen source. The valve 22 and the second flowmeter 42; the oxygen active fragment gas outlet of the modular oxygen active fragment generator 7 is connected with the suction port of the online oxygen active fragment detector 8 and the Venturi gas-liquid miscible device 5 successively, and the online oxygen active fragment The third valve 23 and the third flowmeter 43 are arranged in sequence between the debris detector 8 and the Venturi gas-liquid miscible device 5; the liquid outlet of the Venturi gas-liquid miscible device 5 and the liquid inlet of the liquid-liquid miscible device The liquid outlet of the liquid-liquid miscible device 9 is connected with the TRO detector 10 and the water inlet of the culture pond 13 successively, and the main water inlet pipeline sampling valve 24 is set between the TRO detector 10 and the culture pond 13; in the middle of the culture pond 13 An automatic rotating brush 12 controlled by a motor 11 is provided; the water outlet at the bottom of the culture pond 13 is externally connected to seawater, and the sixth valve 26 of the outer row and the sampling valve 25 of the outer row are arranged between them.

The method for killing and stimulating Cryptocaryon cysts by hydroxyl radicals is given below:

1) Every summer from July to November, when the water temperature is 25-30°C and cryptocystosis is prone to outbreaks, when the cultured fish eat less food, rub the pool wall or bottom with their bodies, the mucus on the body surface increases and there are obvious white spots, And some fish fry died; use a microscope to observe the white spots on the surface of the dead fish, and find that they are black round or pear-shaped solid, which can confirm the occurrence of Cryptocaryoniasis irritant. At 10:00 in the morning, after a large number of cysts came off from the diseased fish, all the diseased fish in the diseased breeding pond 13 were fished out into other clean breeding ponds without disease, and the water level of the diseased breeding pond 13 was reduced. There are a large number of irritating Cryptocaryon cysts. Turn on the high-frequency high-voltage power supply 6 to generate an excitation voltage outside the modular oxygen active fragment generator 7, and open the valve 22 at the same time, and high-purity oxygen enters the modular oxygen active fragment generator 7 through the flow meter 42 to ionize and dissociate to generate For the gas containing oxygen active fragments, 1/15 to 1/10 of the gas containing oxygen active fragments enters the online oxygen active fragment detector 8 to detect the concentration of oxygen active fragments, and the remaining 9/10 to 14/15 enters the Venturi gas-liquid miscibility device 5. The power of the high-frequency high-voltage power supply is 150-300W, and the intake rate of oxygen is 1.0-5.0L/min; the gas flow rate of the oxygen active fragment concentration detector is 0.1-0.5mL/min, and the oxygen active fragment concentration 50-300mg/L.

2) Open the valve 21 and the water pump 31, so that the seawater stored in the water storage tank 1 passes through the flowmeter 41 and then enters the Venturi gas-liquid miscible device 5; the water flow rate is determined according to the number of Cryptocaryon cysts to be treated, which can be 10-50L /min; the gas containing oxygen active fragments prepared in seawater and step 1) is fully mixed in the Venturi gas-liquid miscible device 5 and the liquid-liquid miscible device 9 to generate a high-concentration hydroxyl radical solution, and the concentration of the hydroxyl radical solution is It is regulated by water temperature, oxygen active fragment concentration, equilibrium partial pressure of oxygen-containing active fragment gas, and gas-liquid volume ratio. After the online TRO detector 10 detects the total active oxidant concentration, it enters the culture pond 13. The total oxidant TRO concentration is 2-30mg/ L.

3) After the high-concentration hydroxyl radical solution generated in step 2) is injected into the culture pond 13, the water injection time is 1 hour, the water flow rate is 10-50 L/min, and the water surface height is 0.1-0.5 m; the hydroxyl radical solution soaks and adheres to The Cryptocaryon stimulatory cysts at the bottom of the culture pond 13 were examined for 0.5-2 hours using a microscope to check the death of the Cryptocaryon stimulatory cysts. If the color of the cysts became lighter and there was no obvious cleavage phase, it was judged as dead.

4) After soaking in the hydroxyl radical solution in step 3) to stimulate Cryptocaryon cysts to make them die, turn on the motor 11 to make the automatic rotating brush 12 rotate and work, and the brush rotates along the central axis at a speed of 10r/min; rotate for 10min Close motor 11 at last, open valve 26 and make the dead cyst directly discharge into offshore sea area with seawater after the processing is completed.

The specific examples of killing and stimulating Cryptocaryon cysts are given below:

Hydroxyl radical solution kills and stimulates Cryptocaryon cysts.

In mid-August, in the red sea bream breeding pond of a breeding factory in Xiaodeng Island, Xiamen City, fish fry decreased their feeding intake, rubbed their bodies on the wall or bottom of the pond, increased gill mucus on the body surface with obvious white spots, and some fry died. The water temperature in the culture pond is 27-28°C, the salinity is 25.6‰, the pH is 7.75, and the dissolved oxygen is 6.78mg/L. Through microscopic examination, it was found that there were a large number of black, round or pear-shaped worms wrapped in the mucus on the surface of the diseased fish; and there were granular hard objects at the bottom of the breeding tank, and it was judged that it had cryptocystitis irritating. The stimulated Cryptocaryon cysts were collected at the bottom of the pool for killing experiments, and the killing conditions of stimulated Cryptocaryon cysts under different OH doses were characterized. The number of stimulated Cryptocaryon cysts used in the experiment was 100, the discharge power was 200W, and the concentration of the required hydroxyl radical solution was adjusted by adjusting the gas-liquid miscibility ratio. Four experimental groups were set up, namely the control group and three treatment groups with different concentrations of hydroxyl radicals. The concentrations of the treatment groups were 1.32mg/L, 2.63mg/L and 5.27mg/L. The solutions of different concentrations of hydroxyl radicals were directly passed into the beaker containing the cysts of Cryptocaryon stimuli, and after soaking for 2 hours, the morphology of the cysts of Cryptocaryon stimuli before and after killing by hydroxyl free radicals was observed with a microscope. The results are shown in Figure 2. The cyst shell of the control group was complete, black pear-shaped or round, and obvious split phases could be observed in the cyst; Hatching releases larvae. And concentration is the 5.27mg/L hydroxyl free radical solution treatment after the stimulation Cryptocaryon cyst, its cyst wall is intact substantially, but the cyst color becomes lighter obviously, and there is no cleavage phase to exist; The cyst after treatment is placed in In clean seawater with a temperature of 25.2°C, it was found that the cysts could not continue to develop and release larvae. Table 1 shows the killing effects of different concentrations of hydroxyl radical solutions on stimulating Cryptocaryon cysts.

Table 1 The killing effect of different concentrations of hydroxyl radical solutions on stimulating Cryptocaryon cysts

The hydroxyl free radical solution can oxidize the weak area of the cyst wall to cause holes to enter the interior of the cyst, and the substances and functional substances that make up the basic structure of the cell undergo irreversible reactions such as oxidative decomposition of amino acids and DNA strand breaks, making the cyst dead and unable to develop further The larvae were released, and the remaining hydroxyl radicals were decomposed into O ₂ and H ₂ O. The solution of hydroxyl radicals with a concentration of 5.27mg/L could completely kill and stimulate Cryptocaryon cysts.

Claims (10)

1. a kind of hydroxyl radical free radical, which is killed, stimulates the device of cryptonucleus insect packing, it is characterised in that provided with tank, culturing pool, high frequency High voltage power supply, modularization oxygen activity fragment generator, online oxygen activity fragment detector, venturi gas-Liquid mixing device, liquid liquid are mixed Molten device, TRO detectors, motor, automatic rotating brushes, water pump, valve, gas piping and liquid main line；The modularization oxygen The external source of oxygen of biologically active fragment generator air inlet, the oxygen activity fragment gas outlet of modularization oxygen activity fragment generator successively with The air entry connection of online oxygen activity fragment detector, venturi gas-Liquid mixing device；The tank delivery port and water feeding of water pump Mouth is connected, and pump outlet is connected with venturi gas-Liquid mixing device inlet, liquid outlet and the liquid liquid of venturi gas-Liquid mixing device Dissolving device inlet is connected, and liquid liquid dissolving device liquid outlet is connected with TRO detectors, culturing pool water inlet successively；The culturing pool Centre is provided with the automatic rotating brushes by motor control；The external seawater of culturing pool bottom delivery port.

2. a kind of hydroxyl radical free radical, which is killed, as claimed in claim 1 stimulates the device of cryptonucleus insect packing, it is characterised in that the oxygen Oxygen valve, gas flowmeter are sequentially provided between source of the gas and modularization oxygen activity fragment generator；The online oxygen activity is broken Oxygen-content active fragment gas valve, oxygen-content active fragment gas stream are sequentially provided between piece detector and venturi gas-Liquid mixing device Gauge；Main line Liquid valve is provided between the tank and water pump, liquid is provided between water pump and venturi gas-Liquid mixing device Flowmeter body；Exterior liquid body valve is provided between the culturing pool and external seawater；Set between the TRO detectors and culturing pool Put into water main line sample cock, outer row's sample cock is set between culturing pool and external seawater.

3. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing, it is characterised in that comprise the following steps：

1) annual 7~November of summer, water temperature is when stimulating cryptocaryoniosis easily to break out for 25~30 DEG C, if cultured fishes are ingested Reduce, rubbed pool wall or bottom of pond with body, epidermal mucus increases the situation for having obvious white point and having part fry death；With The white point that micro- sem observation dead fish body surface occurs, it is black circle or pyriform to find it, that is, confirms occur stimulating cryptocaryoniosis； Morning 10：After 00 a large amount of packings come off from sick fish, sick fish in morbidity culturing pool is all pulled out other disease-free clean In culturing pool, water level in reduction morbidity culturing pool, culturing pool bottom of now falling ill is attached with a large amount of stimulation cryptonucleus insect packings；

2) modularization oxygen activity fragment generator switch is opened, oxygen valve is opened and is passed through oxygen, start high-frequency and high-voltage power supply, make Ionized rapidly by the oxygen of modularization oxygen activity fragment generator, be dissociated into gaseous oxygen biologically active fragment；Open oxygen-content active broken Chip valve, gaseous oxygen biologically active fragment is by after line oxygen activity debris concentration detector detects its concentration, into venturi gas-liquid Dissolving device；

3) open storage clean sea water in water pump and main line Liquid valve, tank and venturi gas-liquid is injected by water pump by the road In dissolving device；The step 2) in enter gaseous oxygen biologically active fragment and the seawater of the molten device of venturi gas-liquid mixed in venturi gas-liquid It is fully miscible in dissolving device and liquid liquid dissolving device, high concentration hydroxyl radical solution is produced, it is laggard by TRO detectors by the road Enter in culturing pool；

4) after hydroxyl radical solution injection culturing pool, immersion culturing pool bottom of pond stimulates cryptonucleus insect 0.5~2.0h of packing, and hydroxyl is certainly It hole is occurred hence into packing inside by based sols oxidation package cyst wall weak area, constitute cell basic structure material and Irreversible reaction occurs for functional mass, and the irreversible reaction includes amino-acid oxidase is decomposed, DNA is broken etc., makes packing dead Release larva can not further be developed by dying, and residual hydroxyl free radical resolves into O₂And H₂O；It is provided with the middle of culturing pool by motor control Automatic rotating brushes, automatic rotary cleaning pond packing after startup；Opening exterior liquid body valve after rotation 10min makes in culturing pool Dead packing is discharged with seawater.

4. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step 1) in, the culturing pool is the opening cement pit of class cylinder, and radius is 2m, and depth is 1.5m, and cumulative volume is about 20m³。

5. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step 1) in, culturing pool side top is provided with water inlet, and bottom of pond is the smooth bottom of pond of level, is provided with pond center by the automatic of motor control Rotating brushes, rotation central axis is stainless steel tube, away from there is brush plate at the 0.13m of its bottom, and brush plate length is that 3.8m, width are 0.3m；Brush plate uses the nylon yarn material bristle for being furnished with that length is 0.15m on the smooth brush board of polypropylene material, brush plate；Electricity After machine starts, hairbrush rotates along axis stainless steel tube, and rotating speed is 10r/min, and pool wall side bottom is provided with discharge outlet.

6. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step 2) in, the power of the high-frequency and high-voltage power supply is 150~300W, and oxygen air inflow is 1.0~5.0L/min, and oxygen is through high energy electricity It is ionized, dissociates after son bombardment, forms gaseous oxygen biologically active fragment.

7. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 6, it is characterised in that the gas State oxygen activity fragment includes O₂ ⁺、O(¹D)、O₂(a¹g)、O₂ ^-、O(³) and O P₃Particle, concentration is up to 50~300mg/L；The pipeline is Polyfluortetraethylene pipe.

8. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step 3) in, the flow of the seawater is 10~50L/min, and the total oxidant TRO concentration of hydroxyl radical solution is in 2~30mg/L.

9. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step 3) in, tank is cube enclosed water mud sump, and total measurement (volume) is 20m³, stored seawater is the cleaning sea pre-processed by sand filtration Water.

10. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step 4) in, the stimulation cryptonucleus insect is encapsulated in 0.5~2.0h of immersion in hydroxyl radical solution, is determined by sediments microscope inspection mode After packing is dead, start after automatic rotating brushes switch, rotation 10min, dead packing is discharged with seawater in culturing pool.