CN107027667A - A kind of hydroxyl radical free radical kills the method and apparatus for stimulating cryptonucleus insect packing - Google Patents
A kind of hydroxyl radical free radical kills the method and apparatus for stimulating cryptonucleus insect packing Download PDFInfo
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- CN107027667A CN107027667A CN201710208886.2A CN201710208886A CN107027667A CN 107027667 A CN107027667 A CN 107027667A CN 201710208886 A CN201710208886 A CN 201710208886A CN 107027667 A CN107027667 A CN 107027667A
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- 238000012856 packing Methods 0.000 title claims abstract description 104
- 241000238631 Hexapoda Species 0.000 title claims abstract description 61
- -1 hydroxyl radical free radical Chemical class 0.000 title claims abstract description 35
- 230000004936 stimulating effect Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 93
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 84
- 239000001301 oxygen Substances 0.000 claims abstract description 84
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 84
- 238000012258 culturing Methods 0.000 claims abstract description 61
- 239000012634 fragment Substances 0.000 claims abstract description 58
- 230000000694 effects Effects 0.000 claims abstract description 54
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 47
- 239000013535 sea water Substances 0.000 claims abstract description 33
- 238000002156 mixing Methods 0.000 claims abstract description 30
- 239000007789 gas Substances 0.000 claims abstract description 22
- 241000251468 Actinopterygii Species 0.000 claims description 31
- 239000000243 solution Substances 0.000 claims description 26
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 claims description 22
- 230000000638 stimulation Effects 0.000 claims description 21
- 201000010099 disease Diseases 0.000 claims description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 7
- 239000007800 oxidant agent Substances 0.000 claims description 7
- 206010011732 Cyst Diseases 0.000 claims description 6
- 208000031513 cyst Diseases 0.000 claims description 6
- 238000007654 immersion Methods 0.000 claims description 6
- 230000002427 irreversible effect Effects 0.000 claims description 6
- 230000001590 oxidative effect Effects 0.000 claims description 6
- 230000009467 reduction Effects 0.000 claims description 6
- 210000003097 mucus Anatomy 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 102000004316 Oxidoreductases Human genes 0.000 claims description 4
- 108090000854 Oxidoreductases Proteins 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 238000007689 inspection Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims description 3
- 230000003647 oxidation Effects 0.000 claims description 3
- 238000007254 oxidation reaction Methods 0.000 claims description 3
- 239000004677 Nylon Substances 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 239000004568 cement Substances 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 238000009287 sand filtration Methods 0.000 claims description 2
- 239000013049 sediment Substances 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- QRSFFHRCBYCWBS-UHFFFAOYSA-N [O].[O] Chemical compound [O].[O] QRSFFHRCBYCWBS-UHFFFAOYSA-N 0.000 claims 1
- 230000001186 cumulative effect Effects 0.000 claims 1
- 230000002147 killing effect Effects 0.000 description 10
- 238000011161 development Methods 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 7
- 238000009360 aquaculture Methods 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 5
- 206010042566 Superinfection Diseases 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000002775 capsule Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000009364 mariculture Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000003812 trophozoite Anatomy 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241001663423 Cryptocaryon irritans Species 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 241000798443 Hornodermoporus martius Species 0.000 description 1
- 241000248484 Ichthyophthirius Species 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 238000003723 Smelting Methods 0.000 description 1
- 241001494106 Stenotomus chrysops Species 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004993 binary fission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004081 cilia Anatomy 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910052593 corundum Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000008508 epithelial proliferation Effects 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 230000029052 metamorphosis Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000008816 organ damage Effects 0.000 description 1
- 230000004768 organ dysfunction Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 208000014837 parasitic helminthiasis infectious disease Diseases 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010129 solution processing Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 229910001845 yogo sapphire Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
A kind of hydroxyl radical free radical kills the method and apparatus for stimulating cryptonucleus insect packing, provided with tank, culturing pool, high-frequency and high-voltage power supply, modularization oxygen activity fragment generator, online oxygen activity fragment detector, venturi gas-Liquid mixing device, liquid liquid dissolving device, TRO detectors, motor, automatic rotating brushes, water pump, valve, gas piping and liquid main line；The external source of oxygen of modularization oxygen activity fragment generator air inlet, the air entry of the oxygen activity fragment gas outlet of modularization oxygen activity fragment generator successively with online oxygen activity fragment detector, venturi gas-Liquid mixing device is connected；Tank delivery port is connected with pump intake, pump outlet is connected with venturi gas-Liquid mixing device inlet, the liquid outlet of venturi gas-Liquid mixing device is connected with liquid liquid dissolving device inlet, and liquid liquid dissolving device liquid outlet is connected with TRO detectors, culturing pool water inlet successively；The automatic rotating brushes by motor control is provided with the middle of culturing pool；The external seawater of culturing pool bottom delivery port.
Description
Technical field
The present invention relates to fields such as plasma chemistry, seawater aquaculture technology and marine environment engineerings, especially relate to
And a kind of hydroxyl radical free radical kills the method and apparatus for stimulating cryptonucleus insect packing.
Background technology
It is a kind of infusorian to stimulate cryptonucleus insect, causes seawater fish " ichthyophthirius ", and the disease is cultivation density is too high, mobility
Easily broken out in the abundant water body of the poor, content of organic matter, generally colonize in the skin, fin, the gill of Perenniporia martius fish
Etc., fish organ damage and dysfunction are caused, causes the movable exception of disease fish, epithelial proliferation, expiratory dyspnea and mechanical damage, makes
Fish mortality, huge harm is caused to mariculture industry.
Stimulating the history of life of cryptonucleus insect includes " trophozoite ", " packing precursor ", " packing " and " larva " 4 stages.Nourish
Body is infected to parasitize to grow in disease fish epithelial tissue after sick fish by larva to be formed, and growth period is 3~7d；After after maturation
Depart from host disease fish in the morning and develop into packing precursor；Packing precursor takes off cilium after 8~12h, adheres to water-bed support
Hard shell packing is formed on thing；Packing is by a series of 3~20d asymmetric binary fission formation filial generation larvas, and each packing can
200~400 larvas are discharged, larva moves about in water and infection host fish form trophozoite and carry out next life cycle
(but learn the study on prevention that bright stimulates the passage, preservation and stimulation cryptocaryoniosis of cryptonucleus insect (Cryptocaryon irritans)
[D] Ji'nan University, 2006).
With China's expanding economy, people progressively increase the demand of marine product, rely solely on Conventional marine and fish for
Through that far can not meet the market demand, marine fish culture industry obtains fast development.Stimulate cryptocaryoniosis because its spread speed is fast,
The death rate is high, cause significant damage to mariculture industry the features such as be difficult to preventing and treating.And the hidden core of stimulation of packing Life Stages
Worm has several layers of cyst wall, there is dense matter between layers, and packing outer layer is difficult to crush, and common drug is difficult to enter in packing
Portion, conventional method can not effectively kill packing.Preventing and treating stimulates the key of cryptocaryoniosis to be to kill to stimulate cryptonucleus insect packing, prevents it
The development more larvas of release and superinfection cultured fishes.How to kill high-efficient energy-saving environment friendly stimulation cryptonucleus insect packing by
Increasing concern.Being killed in current sea-farming production frequently with some physics and chemical method stimulates cryptonucleus insect packing.
Physical method mainly has low pressure UV irradiation, freshwater soaking packing, rotation cultivation etc., but effect is not obvious, tends not to
Effectively kill packing.Chemical method includes splashed into ill culturing pool copper sulphate, formalin, liquor potassic permanganate etc., this
Although a little methods have packing certain killing effect, it can not accomplish effectively to kill completely, most of packing still can be after
Supervention educates release larva infection host fish, and also to have medicament residue, the toxicity to fish, packing resistance to for long-term overdose
The problems such as property of medicine enhancing, environmental pollution.Li Anxing etc. removes the method control treatment of packing using shield is laid at culturing pool bottom
Stimulate cryptocaryoniosis (referring to Chinese patent CN105532534A).How to kill high-efficient energy-saving environment friendly stimulation cryptonucleus insect packing by
To increasing concern.
The content of the invention
The purpose of the present invention is to be directed to existing seawater aquaculture moderate stimulation cryptocaryoniosis Onset packing killing effect
It is not good, there is provided can prevent packing development from discharging more to stimulating cryptonucleus insect packing kill the problems such as environmental pollution is serious
A kind of hydroxyl radical free radical of many larva and superinfection cultured fishes kills the method and apparatus for stimulating cryptonucleus insect packing.
A kind of hydroxyl radical free radical kill stimulate the device of cryptonucleus insect packing provided with tank, culturing pool, high-frequency and high-voltage power supply,
Modularization oxygen activity fragment generator, online oxygen activity fragment detector, venturi gas-Liquid mixing device, liquid liquid dissolving device, TRO inspections
Survey instrument, motor, automatic rotating brushes, water pump, valve, gas piping and liquid main line；The modularization oxygen activity fragment hair
The raw external source of oxygen of device air inlet, the oxygen activity fragment gas outlet of modularization oxygen activity fragment generator successively with online oxygen activity
The air entry connection of fragment detector, venturi gas-Liquid mixing device；The tank delivery port is connected with pump intake, water pump
Delivery port is connected with venturi gas-Liquid mixing device inlet, liquid outlet and the liquid liquid dissolving device inlet of venturi gas-Liquid mixing device
It is connected, liquid liquid dissolving device liquid outlet is connected with TRO detectors, culturing pool water inlet successively；It is provided with the middle of the culturing pool by electricity
The automatic rotating brushes of machine control；The external seawater of culturing pool bottom delivery port.
Oxygen valve, gas flowmeter can be sequentially provided between source of oxygen and modularization oxygen activity the fragment generator；
Can be sequentially provided between the online oxygen activity fragment detector and venturi gas-Liquid mixing device oxygen-content active fragment gas valve,
Oxygen-content active fragment gas flowmeter；Main line Liquid valve, water pump and venturi can be provided between the tank and water pump
Fluid flowmeter can be provided between gas-Liquid mixing device；Exterior liquid body valve can be provided between the culturing pool and external seawater；Institute
State and water inlet main line sample cock can be set between TRO detectors and culturing pool, can be set between culturing pool and external seawater outer
Arrange sample cock.
A kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing, comprises the following steps：
1) annual 7~November of summer, water temperature is when stimulating cryptocaryoniosis easily to break out for 25~30 DEG C, if cultured fishes occur
Ingest reduction, rubbed pool wall or bottom of pond with body, epidermal mucus, which increases, to be had obvious white point and has a dead shape of part fry
Condition；The white point occurred with micro- sem observation dead fish body surface, it is black circle or pyriform to find it, you can confirm occur stimulating hidden core
Parasitosis；In the morning 10：After 00 a large amount of packings come off from sick fish, sick fish in morbidity culturing pool is all pulled out other disease-free
In the clean culturing pool of evil, water level in reduction morbidity culturing pool, culturing pool bottom of now falling ill, which is attached with, largely stimulates cryptonucleus insect packing；
2) modularization oxygen activity fragment generator switch is opened, oxygen valve is opened and is passed through oxygen, start high-frequency high-voltage
Source, makes to ionize rapidly by the oxygen of modularization oxygen activity fragment generator, is dissociated into gaseous oxygen biologically active fragment；Open oxygen-containing work
Property fragment valve, gaseous oxygen biologically active fragment is by after line oxygen activity debris concentration detector detects its concentration, into venturi
Gas-Liquid mixing device；
3) open storage clean sea water in water pump and main line Liquid valve, tank and venturi is injected by water pump by the road
In gas-Liquid mixing device；The step 2) in enter gaseous oxygen biologically active fragment and the seawater of the molten device of venturi gas-liquid mixed in venturi
It is fully miscible in gas-Liquid mixing device and liquid liquid dissolving device, high concentration hydroxyl radical solution is produced, passes through TRO detectors by the road
Enter afterwards in culturing pool；
4) after hydroxyl radical solution injection culturing pool, immersion culturing pool bottom of pond stimulates cryptonucleus insect 0.5~2.0h of packing, hydroxyl
Base free-atom aqueous solution oxidation package cyst wall weak area makes it hole occur hence into packing inside, constitutes the thing of cell basic structure
Irreversible reaction occurs for matter and functional mass, and the irreversible reaction includes amino-acid oxidase is decomposed, DNA is broken etc., makes bag
Capsule death can not further develop release larva, and residual hydroxyl free radical resolves into O2And H2O；It is provided with the middle of culturing pool by motor
The automatic rotating brushes of control, automatic rotary cleaning pond packing after startup；Opening exterior liquid body valve after rotation 10min makes cultivation
Dead packing is discharged with seawater in pond.
In step 1) in, the culturing pool is the opening cement pit of class cylinder, and radius is 2m, and depth is 1.5m, overall
Product is about 20m3.Culturing pool side top is provided with water inlet, and bottom of pond is the smooth bottom of pond of level, is provided with pond center by motor control
Automatic rotating brushes, rotation central axis is stainless steel tube, away from there is brush plate at the 0.13m of its bottom, brush plate be length be 3.8m,
Width is the 0.3m smooth brush board of polypropylene material, is furnished with length for 0.15m nylon yarn material bristles；After electric motor starting, hairbrush
It can be rotated along axis stainless steel tube, rotating speed is 10r/min, pool wall side bottom is provided with discharge outlet.
In step 2) in, the power of the high-frequency and high-voltage power supply is 150~300W, and oxygen air inflow is 1.0~5.0L/
Min, oxygen is ionized after being bombarded through high energy electron, dissociated, and forms gaseous oxygen biologically active fragment, and the gaseous oxygen biologically active fragment is main
Including O2 +、O(1D)、O2(a1g)、O2 -、O(3) and O P3Deng particle, concentration is up to 50~300mg/L；The pipeline is polytetrafluoroethyl-ne
Alkene pipe.
In step 3) in, the flow of the seawater is 10~50L/min, and the concentration of hydroxyl radical solution is by water temperature, oxygen
Biologically active fragment concentration, the equilibrium partial pressure of oxygen-content active fragment gas and gas-liquid volume ratio regulate and control, and total oxidant TRO concentration is 2
~30mg/L；Tank is cube enclosed water mud sump, and total measurement (volume) is about 20m3, stored seawater is by the pretreatment such as sand filtration
Clean sea water.
In step 4) in, the stimulation cryptonucleus insect is encapsulated in 0.5~2.0h of immersion in hydroxyl radical solution, by micro-
After the modes such as mirror microscopy determine that packing is dead, start after automatic rotating brushes switch, rotation 10min, dead packing in culturing pool
Discharged with seawater, liquid line used can use the stainless steel pipe through preservative treatment, the culturing pool after processing can continue to support
Breeding fish.
Oxygen is ionized in pole narrow discharge gap, is dissociated into high concentration oxygen activity by the present invention using atmospheric pressure ionic discharge
Fragment, then hydroxyl radical solution is efficiently and rapidly generated in 1s by gas-Liquid mixing, the miscible effect of liquid liquid.Hydroxyl radical free radical
Solution effects cyst wall weak area makes it hole occur hence into packing inside, constitutes the material and function of cell basic structure
Such as amino-acid oxidase decomposition, DNA fracture irreversible reaction occur for material, packing death is prevented that its development release is more
Larva and superinfection cultured fishes, the effect of cryptocaryoniosis is stimulated so as to reach and administer.Hydroxyl free solution is killing stimulation
While cryptonucleus insect packing, the microorganism such as remaining harmful bacteria in culturing pool can quickly be killed, prevent and treat the hair of pest and disease damage
It is raw.
The hydroxyl radical free radical of the present invention is killed in the device for stimulating cryptonucleus insect packing, and oxidant concentration is by water temperature, oxygen activity
Debris concentration, the equilibrium partial pressure of oxygen-content active fragment gas and gas-liquid volume ratio regulate and control；Adhered to according to disease incidence and bottom of pond
Packing quantity determines the concentration and soak time of hydroxyl radical solution, and concentration is molten for 5.0~10.0mg/L hydroxyl radical free radical
Liquid can efficiently kill stimulation cryptonucleus insect packing in 0.5~2.0h, it can not be continued development and form larva.
The present invention is killed using the hydroxyl radical free radical (OH) generated based on atmospheric pressure ionic discharge stimulates cryptonucleus insect bag
The method of capsule, is mainly used in seawater aquaculture and kills stimulation cryptonucleus insect packing aspect, use the hydroxyl based on high-level oxidation technology
Base free radical (OH), OH is green strong oxidizer, with it is quick, efficient, can be reacted with large biological molecule, it is and raw after reaction
Into free of contamination H2O and O2, the characteristic such as no Residuals.Being killed based on hydroxyl radical free radical stimulates the method and dress of cryptonucleus insect packing
Being set to seawater aquaculture preventing and treating stimulates cryptocaryoniosis to provide new solution route.
The present invention technique effect and advantage be：1. oxygen is passed into pole narrow discharge gap 0.1mm, sub-micron
Al2O3Smelting is pasted into the miniaturization non-equilibrium plasma source of thin dielectric layer, realizes the generation of atmospheric pressure ionic discharge highly concentrated
Spend oxygen active particles；Using the integrated source of modular array formula plasma and it is combined, obtains controllable, the oxygen of big yield
Active particle, cooperates with water jet cavitation gas-Liquid mixing technology, obtains high concentration hydroxyl radical solution, total oxidant TRO concentration
In 2.0~30.0mg/L；2. adhere to culturing pool bottom stimulation cryptonucleus insect be encapsulated in concentration be 5.0~10.0mg/L hydroxyl from
0.5~2.0h is soaked in based sols, can be achieved to stimulate the complete killing of cryptonucleus insect packing, the more childrens of its development release are prevented
Worm and superinfection cultured fishes；3. hydroxyl free solution can be carried out while stimulation cryptonucleus insect packing is killed to culturing pool
Disinfection, pre- preventing disease and pest；4. can thoroughly be removed using automatic rotating brushes adhere to culturing pool bottom dead stimulate it is hidden
Core worm packing, dead packing is directly discharged with residual seawater in pond；5. to implement simple, operation cost low for the invention described device,
Drug residue free, environmental nonpollution non-toxic to fish, are seawater aquaculture while efficient killing stimulates cryptonucleus insect packing
The killing of moderate stimulation cryptonucleus insect packing provides the efficient device of health environment-friendly.
Brief description of the drawings
Fig. 1 is the structure composition signal that hydroxyl radical free radical of the present invention kills the device embodiment for stimulating cryptonucleus insect packing
Figure.
Packing metamorphosis before and after Fig. 2 kills for the hydroxyl radical free radical of the embodiment of the present invention.In fig. 2, A, C are control group
Packing form photo；Packing form photo when B, D are TRO=5.27mg/L.
Embodiment
With reference to accompanying drawing, the invention will be further described.But protection scope of the present invention is not limited to the embodiment.
Referring to Fig. 1, the hydroxyl radical free radical, which is quickly killed, stimulates the device of cryptonucleus insect packing, provided with tank 1, venturi
Gas-Liquid mixing device 5, high-frequency and high-voltage power supply 6, modularization oxygen activity fragment generator 7, online oxygen activity fragment detector 8, liquid liquid
Dissolving device 9, TRO detectors 10, motor 11, automatic rotating brushes 12 and culturing pool 13.
The delivery port of tank 1 is connected with the water inlet of water pump 31, and the 1st valve 21 is provided between tank 1 and water pump 31；
The delivery port of water pump 31 is connected with the inlet of venturi gas-Liquid mixing device 5, and is set between water pump 31 and venturi gas-Liquid mixing device 5
1 flowmeter 41；The air inlet of modularization oxygen activity fragment generator 7 connects external source of oxygen, modularization oxygen activity fragment generator 7 with
The 2nd valve 22 and the 2nd flowmeter 42 are set gradually between source of oxygen；The oxygen activity fragment of modularization oxygen activity fragment generator 7
Air entry of the gas outlet successively with online oxygen activity fragment detector 8, venturi gas-Liquid mixing device 5 is connected, and online oxygen activity is broken
The 3rd valve 23, the 3rd flowmeter 43 are set gradually between piece detector 8 and venturi gas-Liquid mixing device 5；Venturi gas-Liquid mixing
The liquid outlet of device 5 is connected with the inlet of liquid liquid dissolving device 9；The liquid outlet of liquid liquid dissolving device 9 successively with TRO detectors 10, support
The water inlet connection of pond 13 is grown, water inlet main line sample cock 24 is set between TRO detectors 10 and culturing pool 13；In culturing pool 13
Between provided with the automatic rotating brushes 12 that is controlled by motor 11；The external seawater of bottom delivery port of culturing pool 13, between set and outer arrange the 6th
Valve 26 and outer row's sample cock 25.
Hydroxyl radical free radical given below kills the method for stimulating cryptonucleus insect packing：
1) annual 7~November of summer, water temperature is when stimulating cryptocaryoniosis easily to break out for 25~30 DEG C, when cultured fishes occur
Ingest reduction, rubbed pool wall or bottom of pond with body, epidermal mucus, which increases, to be had obvious white point and has a dead shape of part fry
Condition；The white point occurred with micro- sem observation dead fish body surface, it is black circle or pyriform solid fraction to find it, you can confirmation is stimulated
Cryptocaryoniosis.In the morning 10：After 00 a large amount of packings come off from sick fish, sick fish in morbidity culturing pool 13 is all pulled out it
In its disease-free clean culturing pool, the reduction morbidity water level of culturing pool 13, the bottom of culturing pool 13 of now falling ill is attached with a large amount of stimulations
Cryptonucleus insect packing.High-frequency and high-voltage power supply 6 is opened, makes modularization oxygen activity fragment generator 7 is outer to produce driving voltage, opens simultaneously
Valve 22, high-purity oxygen enters modularization oxygen activity fragment generator 7 by flowmeter 42 makes it be ionized, dissociated, and produces
The raw gas containing oxygen activity fragment, 1/15~1/10 gas containing oxygen activity fragment enters online oxygen activity fragment detector
8 detect oxygen activity debris concentration, and remaining 9/10~14/15 enters venturi gas-Liquid mixing device 5.The high-frequency and high-voltage power supply
Power is 150~300W, and the air inflow of oxygen is 1.0~5.0L/min；The oxygen activity debris concentration detector gas flow
For 0.1~0.5mL/min, the oxygen activity debris concentration is 50~300mg/L.
2) valve 21 and water pump 31 are opened, makes to store seawater in tank 1 by entering venturi gas-liquid after flowmeter 41
Dissolving device 5；Water-carrying capacity is determined according to pending stimulation cryptonucleus insect packing quantity, can be 10~50L/min；Seawater and step 1) in
The gas containing oxygen activity fragment prepared generates high after being sufficiently mixed in venturi gas-Liquid mixing device 5 and liquid liquid dissolving device 9
Concentration hydroxyl radical solution, hydroxyl radical solution concentration is by water temperature, oxygen activity debris concentration, oxygen-content active fragment gas
Equilibrium partial pressure and gas-liquid volume ratio regulate and control, by entering cultivation after the detection gross activity oxidant concentration of line TRO detectors 10
Pond 13, total oxidant TRO concentration is in 2~30mg/L.
3) step 2) in generation high concentration hydroxyl radical solution injection culturing pool 13 after, water injection time is 1h, current
Measure as 10~50L/min, water surface elevation is 0.1~0.5m；Hydroxyl radical solution soaks the stimulation for sticking to the bottom of culturing pool 13
Cryptonucleus insect 0.5~2h of packing, checks stimulation cryptonucleus insect packing death condition, if packing lighter using sediments microscope inspection mode
And be to be judged as death without obvious split coil method.
4) step 3) in hydroxyl radical solution immersion stimulate cryptonucleus insect packing make after its death, open motor 11, make from
The dynamic rotary work of rotating brushes 12, hairbrush is rotated along axis with 10r/min rotating speed；Rotate and motor 11 is closed after 10min, open
Valve 26 makes packing dead after the completion of processing be directly discharged into offshore sea waters with seawater.
Given below kill stimulates cryptonucleus insect packing specific embodiment：
Hydroxyl radical solution, which is killed, stimulates the experiment of cryptonucleus insect packing.
Occur in mid-August, Xiamen City little Deng islands cultivation factory porgy culturing pool fry ingest reductions, with body friction pond
Wall or bottom of pond, body surface gill portion mucus, which increase, to be had obvious white point and has a situations such as part fry death.Its culturing pool water temperature be 27~
28 DEG C, salinity is that 25.6 ‰, pH is 7.75, and dissolved oxygen is 6.78mg/L.Find to wrap in sick fish epidermal mucus by microscope inspection
It is wrapped with that a large amount of black, shape be rounded or polypide of pyriform；And culturing pool bottom is with the presence of particle adamant, judge that it is pierced
Swash cryptocaryoniosis.Collecting pit bottom stimulates cryptonucleus insect packing to carry out killing test, to stimulating cryptonucleus insect packing under different OH dosage
Killing situation characterized.Experiment is used to stimulate cryptonucleus insect packing number to be 100, and discharge power is 200W, passes through regulation
Gas-Liquid mixing ratio is come hydroxyl radical solution concentration needed for adjusting.Four experimental groups, respectively control group and three differences are set
Concentration hydroxyl radical free radical treatment group, treatment group concentration is 1.32mg/L, 2.63mg/L and 5.27mg/L.By various concentrations hydroxyl certainly
It is passed directly into equipped with the beaker for stimulating cryptonucleus insect packing, hydroxyl radical free radical is killed using microscope by based sols after immersion 2h
Front and rear stimulation cryptonucleus insect packing form is observed.As a result as shown in Fig. 2 control group capsule shell is complete, in black pyriform or
Obvious split coil method can be observed in circle, and packing；Packing is positioned over a period of time in the clean seawater that temperature is 25.2 DEG C
It was found that release larva can be hatched.And concentration is the stimulation cryptonucleus insect packing after 5.27mg/L hydroxyl radical solution processing, its
Cyst wall is substantially complete, but packing color substantially shoals, and exists without split coil method；It is 25.2 that packing after processing is positioned over into temperature
DEG C clean seawater in find packing can not continue development release larva.Various concentrations hydroxyl radical solution is to stimulating cryptonucleus insect
The killing effect of packing is as shown in table 1.
The various concentrations hydroxyl radical solution of table 1 is to stimulating the killing effect of cryptonucleus insect packing
The oxidable cyst wall weak area of hydroxyl radical solution makes it hole occur hence into packing inside, constitutes cell
Such as amino-acid oxidase decomposition, DNA fracture irreversible reaction occur for the material and functional mass of basic structure, make packing dead
Release larva can not be further developed, residual hydroxyl free radical resolves into O2And H2O, concentration is 5.27mg/L hydroxyl radical free radical
Solution can complete lethal stimulation cryptonucleus insect packing.
Claims (10)
1. a kind of hydroxyl radical free radical, which is killed, stimulates the device of cryptonucleus insect packing, it is characterised in that provided with tank, culturing pool, high frequency
High voltage power supply, modularization oxygen activity fragment generator, online oxygen activity fragment detector, venturi gas-Liquid mixing device, liquid liquid are mixed
Molten device, TRO detectors, motor, automatic rotating brushes, water pump, valve, gas piping and liquid main line；The modularization oxygen
The external source of oxygen of biologically active fragment generator air inlet, the oxygen activity fragment gas outlet of modularization oxygen activity fragment generator successively with
The air entry connection of online oxygen activity fragment detector, venturi gas-Liquid mixing device；The tank delivery port and water feeding of water pump
Mouth is connected, and pump outlet is connected with venturi gas-Liquid mixing device inlet, liquid outlet and the liquid liquid of venturi gas-Liquid mixing device
Dissolving device inlet is connected, and liquid liquid dissolving device liquid outlet is connected with TRO detectors, culturing pool water inlet successively；The culturing pool
Centre is provided with the automatic rotating brushes by motor control；The external seawater of culturing pool bottom delivery port.
2. a kind of hydroxyl radical free radical, which is killed, as claimed in claim 1 stimulates the device of cryptonucleus insect packing, it is characterised in that the oxygen
Oxygen valve, gas flowmeter are sequentially provided between source of the gas and modularization oxygen activity fragment generator；The online oxygen activity is broken
Oxygen-content active fragment gas valve, oxygen-content active fragment gas stream are sequentially provided between piece detector and venturi gas-Liquid mixing device
Gauge；Main line Liquid valve is provided between the tank and water pump, liquid is provided between water pump and venturi gas-Liquid mixing device
Flowmeter body；Exterior liquid body valve is provided between the culturing pool and external seawater；Set between the TRO detectors and culturing pool
Put into water main line sample cock, outer row's sample cock is set between culturing pool and external seawater.
3. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing, it is characterised in that comprise the following steps：
1) annual 7~November of summer, water temperature is when stimulating cryptocaryoniosis easily to break out for 25~30 DEG C, if cultured fishes are ingested
Reduce, rubbed pool wall or bottom of pond with body, epidermal mucus increases the situation for having obvious white point and having part fry death；With
The white point that micro- sem observation dead fish body surface occurs, it is black circle or pyriform to find it, that is, confirms occur stimulating cryptocaryoniosis；
Morning 10：After 00 a large amount of packings come off from sick fish, sick fish in morbidity culturing pool is all pulled out other disease-free clean
In culturing pool, water level in reduction morbidity culturing pool, culturing pool bottom of now falling ill is attached with a large amount of stimulation cryptonucleus insect packings；
2) modularization oxygen activity fragment generator switch is opened, oxygen valve is opened and is passed through oxygen, start high-frequency and high-voltage power supply, make
Ionized rapidly by the oxygen of modularization oxygen activity fragment generator, be dissociated into gaseous oxygen biologically active fragment；Open oxygen-content active broken
Chip valve, gaseous oxygen biologically active fragment is by after line oxygen activity debris concentration detector detects its concentration, into venturi gas-liquid
Dissolving device；
3) open storage clean sea water in water pump and main line Liquid valve, tank and venturi gas-liquid is injected by water pump by the road
In dissolving device；The step 2) in enter gaseous oxygen biologically active fragment and the seawater of the molten device of venturi gas-liquid mixed in venturi gas-liquid
It is fully miscible in dissolving device and liquid liquid dissolving device, high concentration hydroxyl radical solution is produced, it is laggard by TRO detectors by the road
Enter in culturing pool；
4) after hydroxyl radical solution injection culturing pool, immersion culturing pool bottom of pond stimulates cryptonucleus insect 0.5~2.0h of packing, and hydroxyl is certainly
It hole is occurred hence into packing inside by based sols oxidation package cyst wall weak area, constitute cell basic structure material and
Irreversible reaction occurs for functional mass, and the irreversible reaction includes amino-acid oxidase is decomposed, DNA is broken etc., makes packing dead
Release larva can not further be developed by dying, and residual hydroxyl free radical resolves into O2And H2O；It is provided with the middle of culturing pool by motor control
Automatic rotating brushes, automatic rotary cleaning pond packing after startup；Opening exterior liquid body valve after rotation 10min makes in culturing pool
Dead packing is discharged with seawater.
4. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step
1) in, the culturing pool is the opening cement pit of class cylinder, and radius is 2m, and depth is 1.5m, and cumulative volume is about 20m3。
5. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step
1) in, culturing pool side top is provided with water inlet, and bottom of pond is the smooth bottom of pond of level, is provided with pond center by the automatic of motor control
Rotating brushes, rotation central axis is stainless steel tube, away from there is brush plate at the 0.13m of its bottom, and brush plate length is that 3.8m, width are
0.3m；Brush plate uses the nylon yarn material bristle for being furnished with that length is 0.15m on the smooth brush board of polypropylene material, brush plate；Electricity
After machine starts, hairbrush rotates along axis stainless steel tube, and rotating speed is 10r/min, and pool wall side bottom is provided with discharge outlet.
6. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step
2) in, the power of the high-frequency and high-voltage power supply is 150~300W, and oxygen air inflow is 1.0~5.0L/min, and oxygen is through high energy electricity
It is ionized, dissociates after son bombardment, forms gaseous oxygen biologically active fragment.
7. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 6, it is characterised in that the gas
State oxygen activity fragment includes O2 +、O(1D)、O2(a1g)、O2 -、O(3) and O P3Particle, concentration is up to 50~300mg/L；The pipeline is
Polyfluortetraethylene pipe.
8. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step
3) in, the flow of the seawater is 10~50L/min, and the total oxidant TRO concentration of hydroxyl radical solution is in 2~30mg/L.
9. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step
3) in, tank is cube enclosed water mud sump, and total measurement (volume) is 20m3, stored seawater is the cleaning sea pre-processed by sand filtration
Water.
10. a kind of hydroxyl radical free radical kills the method for stimulating cryptonucleus insect packing as claimed in claim 3, it is characterised in that in step
4) in, the stimulation cryptonucleus insect is encapsulated in 0.5~2.0h of immersion in hydroxyl radical solution, is determined by sediments microscope inspection mode
After packing is dead, start after automatic rotating brushes switch, rotation 10min, dead packing is discharged with seawater in culturing pool.
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| CN201710208886.2A CN107027667B (en) | 2017-03-31 | 2017-03-31 | Method and device for killing cryptocaryon irritans cysts by hydroxyl free radicals |
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| CN111183935A (en) * | 2018-11-15 | 2020-05-22 | 中山大学 | Method for controlling cryptocaryon irritans disease of fish by using ozone |
| CN114403060A (en) * | 2021-12-13 | 2022-04-29 | 山东耕海海洋科技有限公司 | Culture system for preventing and treating cryptocaryon disease by using jellyfish and disease treatment method |
| CN114651771A (en) * | 2020-12-08 | 2022-06-24 | 申广彦 | Epinephelus coioides breeding treatment system |
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| CN105660462A (en) * | 2015-12-18 | 2016-06-15 | 中山大学 | Cyst inactivation method for controlling cryptocaryon irritans disease of fishes |
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| CN114651771A (en) * | 2020-12-08 | 2022-06-24 | 申广彦 | Epinephelus coioides breeding treatment system |
| CN114403060A (en) * | 2021-12-13 | 2022-04-29 | 山东耕海海洋科技有限公司 | Culture system for preventing and treating cryptocaryon disease by using jellyfish and disease treatment method |
| CN114403060B (en) * | 2021-12-13 | 2022-11-15 | 山东耕海海洋科技有限公司 | Culture system for preventing and treating cryptocaryon disease by using jellyfish and disease treatment method |
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