CN107027667B - Method and device for killing cryptocaryon irritans cysts by hydroxyl free radicals - Google Patents

Abstract

A method and a device for killing cryptocaryon irritans cysts by hydroxyl free radicals are provided with a water storage pool, a culture pool, a high-frequency high-voltage power supply, a modular oxygen active fragment generator, an online oxygen active fragment detector, a Venturi gas-liquid mixing and dissolving device, a liquid-liquid mixing and dissolving device, a TRO detector, a motor, an automatic rotating brush, a water pump, a valve, a gas pipeline and a liquid main pipeline; an air inlet of the modularized oxygen active fragment generator is externally connected with an oxygen source, and an oxygen active fragment air outlet of the modularized oxygen active fragment generator is sequentially connected with an online oxygen active fragment detector and an air suction port of the venturi air-liquid mixing and dissolving device; the water outlet of the water storage tank is connected with the water inlet of a water pump, the water outlet of the water pump is connected with the liquid inlet of a Venturi gas-liquid mixing and dissolving device, the liquid outlet of the Venturi gas-liquid mixing and dissolving device is connected with the liquid inlet of a liquid-liquid mixing and dissolving device, and the liquid outlet of the liquid-liquid mixing and dissolving device is sequentially connected with a TRO detector and the water inlet of a culture pond; the middle of the culture pond is provided with an automatic rotating brush controlled by a motor; the water outlet at the bottom of the culture pond is externally connected with seawater.

Description

A kind of method and device for killing and stimulating Cysts of Cryptonucleus spp. by hydroxyl radicals

technical field

The invention relates to the fields of plasma chemistry, seawater aquaculture technology, marine environmental engineering and the like, in particular to a method and a device for killing and stimulating cysts of Cryptonucleidium by hydroxyl radicals.

Background technique

Cryptonuclearia stimuli is a ciliate that causes "white spot disease" in marine fish. The disease is prone to outbreaks in waters with high breeding density, poor mobility and rich organic matter content, and usually parasitizes tropical and subtropical marine fish The skin, fins, gills, etc. of the fish will cause damage and dysfunction of fish organs, cause abnormal activities of diseased fish, epithelial hyperplasia, breathing difficulties and mechanical damage, and cause a large number of fish deaths, causing great harm to the marine aquaculture industry.

The life cycle of C. stimuli includes four stages: "trophozoite", "cyst precursor", "cyst" and "larva". The trophozoite is formed by larvae infecting the diseased fish and then parasitizing in the epithelial tissue of the diseased fish. The cyst precursor loses its cilia and adheres to the underwater support to form a hard shell cyst; the cyst undergoes a series of asymmetric binary divisions for 3 to 20 days to form progeny larvae, and each cyst can release 200 to 400 cysts Larvae, larvae swim in the water and infect the host fish to form trophozoites for the next life cycle (Dan Xueming. Research on the passage, preservation and control of stimulated Cryptocaryon irritans) [D]. Jinan University, 2006).

With the development of my country's economy, people's demand for seafood has gradually increased, and only relying on traditional marine fishing is far from being able to meet the market demand, and the marine fish aquaculture industry has developed rapidly. C. irritans has caused great harm to the mariculture industry due to its characteristics of rapid spread, high mortality, and difficulty in prevention and control. However, in the life cycle stage of cysts, Cryptonuclearia irritans has several layers of cyst walls, and there are dense substances between the layers. . The key to the prevention and treatment of C. stimuli is to kill the cysts of C. stimuli to prevent them from developing and releasing more larvae and reinfecting farmed fish. How to kill the cysts of C. irritans in an efficient, energy-saving and environmentally friendly way has received more and more attention. At present, some physical and chemical methods are often used in marine aquaculture production to kill C. irritans cysts. Physical methods mainly include low-pressure ultraviolet irradiation, freshwater immersion cysts, and rotation breeding, etc., but the effect is not obvious and often cannot effectively kill cysts. Chemical methods include pouring copper sulfate, formalin, potassium permanganate solution, etc. into the diseased breeding pond. Although these methods have a certain killing effect on cysts, they cannot be effectively and completely killed. Cysts can still continue to develop and release larvae to infect host fish, and long-term overdose will also cause problems such as drug residues, toxicity to fish, increased drug resistance of cysts, and environmental pollution. Li Anxing et al. used the method of laying a cloth at the bottom of the breeding pond to remove the cysts to control and treat the irritable cryptorhizobia (see Chinese patent CN105532534A). How to kill the cysts of C. irritans in an efficient, energy-saving and environmentally friendly way has received more and more attention.

SUMMARY OF THE INVENTION

The purpose of the present invention is to solve the problems such as poor cyst killing effect and serious environmental pollution during the outbreak period of stimulatory cryptonium worm disease in the existing seawater aquaculture, provide that the cysts of stimuli stimuli can be killed and prevent cysts. A method and device for killing and stimulating Cysts of Cryptonucleus spp. by hydroxyl radicals that develop and release more larvae to re-infect farmed fish.

A device for killing and stimulating cysts of Cryptonucleidium by hydroxyl radicals is provided with a water storage tank, a breeding tank, a high-frequency high-voltage power supply, a modular oxygen-active fragment generator, an online oxygen-active fragment detector, and a venturi gas-liquid mixer. , liquid-liquid mixer, TRO detector, motor, automatic rotating brush, water pump, valve, gas pipeline and liquid main pipeline; the air inlet of the modular oxygen active debris generator is connected to an oxygen source, and the modular oxygen active The oxygen-active debris outlet of the debris generator is sequentially connected with the online oxygen-active debris detector and the suction port of the venturi gas-liquid mixer; the water outlet of the water storage tank is connected with the water inlet of the water pump, and the water outlet of the water pump is connected with the venturi gas The liquid-liquid mixer is connected to the liquid inlet, the liquid outlet of the Venturi gas-liquid mixer is connected to the liquid-liquid mixer, and the liquid-liquid mixer is connected to the TRO detector and the water inlet of the breeding tank in turn. There is an automatic rotating brush controlled by a motor in the middle of the cultivating pond; seawater is externally connected to the water outlet at the bottom of the cultivating pond.

An oxygen valve and a gas flow meter can be arranged in sequence between the oxygen source and the modular oxygen active debris generator; an oxygen-containing active debris detector can be arranged in sequence between the online oxygen active debris detector and the venturi gas-liquid mixer. Fragment gas valve and oxygen-containing active fragment gas flow meter; a main pipeline liquid valve can be arranged between the water storage tank and the water pump, and a liquid flow meter can be arranged between the water pump and the venturi gas-liquid mixer; the culture pond An external discharge liquid valve can be provided between the external seawater; a water inlet main pipeline sampling valve can be set between the TRO detector and the breeding pond, and an external discharge sampling valve can be set between the breeding pond and the external seawater.

A method for killing and stimulating Cysts of Cryptocaryonidium by hydroxyl radicals, comprising the following steps:

1) From July to November every summer, when the water temperature is between 25 and 30 °C to stimulate the outbreak of cryptonium disease, if the fish feed decreases, the body rubs against the wall or bottom of the pool, the surface mucus increases, and there are obvious white spots. And some fry died; observe the white spots on the surface of the dead fish with a microscope, and find that they are black round or pear-shaped, which can confirm the occurrence of irritable cryptorhizobia; at 10:00 in the morning, a large number of cysts are diseased. After the fish fall off, remove all the diseased fish in the diseased breeding pond to other clean breeding ponds without disease to reduce the water level in the diseased breeding pond.

2) Turn on the switch of the modular oxygen active fragment generator, open the oxygen valve to introduce oxygen, and start the high-frequency high-voltage power supply, so that the oxygen passing through the modular oxygen active fragment generator is rapidly ionized and dissociated into gaseous oxygen active fragments; open the oxygen-containing active fragment Fragment valve, after the gaseous oxygen active fragments are detected by the online oxygen active fragment concentration detector, they enter the Venturi gas-liquid mixer;

3) open the water pump and the main pipeline liquid valve, and store clean seawater in the water storage tank and inject the water pump into the venturi gas-liquid mixer through the pipeline; the gaseous oxygen active fragments and seawater entering the venturi gas-liquid mixer in the described step 2) Fully miscible in the Venturi gas-liquid mixer and liquid-liquid mixer to generate a high-concentration hydroxyl radical solution, which enters the culture tank after passing through the TRO detector through the pipeline;

4) After the hydroxyl radical solution is injected into the culture pond, soak the bottom of the culture pond to stimulate the cysts of Cryptonuclear worms for 0.5-2.0 hours, and the hydroxyl radical solution oxidizes the weak area of the cyst wall to make holes appear inside the cyst, and the basic cells are formed. Structural substances and functional substances undergo irreversible reactions, including oxidative decomposition of amino acids, DNA strand breaks, etc., so that the cysts die and cannot develop further to release larvae, and the remaining hydroxyl radicals are decomposed into O ₂ and H ₂ O; There is an automatic rotating brush controlled by a motor, which automatically rotates and cleans the cysts in the pool after starting; after 10 minutes of rotation, the liquid discharge valve is opened to discharge the dead cysts in the culture pool with the seawater.

In step 1), the culturing pond is a cylindrical-like open cement pond, with a radius of 2 m, a depth of 1.5 m, and a total volume of about 20 m ³ . There is a water inlet on the top of the side of the breeding pond, the bottom of the pond is horizontal and smooth, and there is an automatic rotating brush controlled by a motor in the center of the pond. The brush plate is a smooth polypropylene brush plate with a length of 3.8m and a width of 0.3m, and is covered with bristles made of nylon wire with a length of 0.15m; after the motor is started, the brush can rotate along the stainless steel tube of the central axis, and the speed is 10r/min , There is a drainage outlet at the bottom of the side of the pool wall.

In step 2), the power of the high-frequency high-voltage power supply is 150-300W, the intake air volume of oxygen is 1.0-5.0L/min, and the oxygen is ionized and dissociated after being bombarded by high-energy electrons to form gaseous oxygen active fragments. The gaseous oxygen active fragments mainly include particles such as O ₂ ⁺ , O( ¹ D), O ₂ (a ¹ g), O ₂ ^- , O( ³ P) and O ₃ , and the concentration can reach 50-300 mg/L; The pipeline is a polytetrafluoroethylene pipe.

In step 3), the flow rate of the seawater is 10-50L/min, the concentration of the hydroxyl radical solution is regulated by the water temperature, the concentration of oxygen-active fragments, the equilibrium partial pressure of the oxygen-containing active fragment gas and the gas-liquid volume ratio, and the total oxidant The concentration of TRO is 2-30 mg/L; the water storage tank is a cubic closed cement tank with a total volume of about 20 m ³ , and the stored seawater is clean seawater that has been pretreated by sand filtration.

In step 4), the cysts of the stimulated Cryptocarpus are soaked in the hydroxyl radical solution for 0.5 to 2.0 hours, and after confirming that the cysts are dead by means such as microscopic examination, the automatic rotating brush switch is activated, and after rotating for 10 minutes, the culture is carried out. The dead cysts in the pond are discharged with the sea water, and the liquid pipeline used can be made of stainless steel pipes with anti-corrosion treatment, and the treated pond can continue to breed fish.

The invention utilizes atmospheric pressure ionization discharge to ionize and dissociate oxygen into high-concentration oxygen-active fragments in an extremely narrow discharge gap, and then efficiently and rapidly generates a hydroxyl radical solution within 1s through gas-liquid miscibility and liquid-liquid miscibility. The hydroxyl radical solution acts on the weak area of the cyst wall to cause holes to enter the cyst, and the substances and functional substances that make up the basic structure of the cell undergo irreversible reactions such as amino acid oxidative decomposition, DNA strand breakage, etc., so that the cyst dies and prevents its development and release. More larvae and repeated infection of farmed fish, so as to achieve the effect of controlling stimulatory cryptonium worm disease. The hydroxyl free solution can quickly kill microorganisms such as residual harmful bacteria in the breeding pond while killing the cysts of the irritant Cryptocaryonidium, so as to prevent the occurrence of diseases and insect pests.

In the device for killing and stimulating the cysts of Cryptonuclear worms by hydroxyl radicals of the present invention, the concentration of the oxidant is regulated by the water temperature, the concentration of oxygen active fragments, the equilibrium partial pressure of the oxygen-containing active fragment gas and the gas-liquid volume ratio; The number of attached cysts determines the concentration and soaking time of the hydroxyl radical solution. The hydroxyl radical solution with a concentration of 5.0 to 10.0 mg/L can effectively kill and stimulate the cysts of Cryptonaria in 0.5 to 2.0 hours, making it unable to continue to develop. form larvae.

The present invention uses the hydroxyl radical ( OH) generated based on atmospheric pressure ionization discharge to kill the cyst of Cryptocarium stimuli, which is mainly used for killing the cyst of C. stimuli in seawater aquaculture. Hydroxyl radicals ( _· OH), _· OH are green strong oxidants, which are fast, efficient, and can react with biological macromolecules. The method and device for killing the cysts of C. stimuli based on hydroxyl radicals provide a new solution for the prevention and control of C. stimuli in seawater aquaculture.

The technical effects and advantages of the present invention are as follows: (1) Oxygen is introduced into a miniaturized non-equilibrium plasma source with a very narrow discharge gap of 0.1 mm and sub-micron Al ₂ O ₃ smelted into a thin dielectric layer, thereby achieving atmospheric pressure strong ionization Generate high-concentration oxygen-active particles by discharge; use modular array plasma integrated sources and any combination to obtain adjustable, large-volume oxygen-active particles, and cooperate with water jet cavitation gas-liquid miscibility technology to obtain high-concentration hydroxyl radicals Solution, the total oxidant TRO concentration is 2.0 ~ 30.0mg/L; ②The cysts of Cryptonucleus irritans adhering to the bottom of the culture tank are soaked in the hydroxyl radical solution with a concentration of 5.0 ~ 10.0mg/L for 0.5 ~ 2.0h, and the Realize the complete killing of the cysts of C. stimuli, preventing them from developing and releasing more larvae and re-infecting farmed fish; ③The hydroxyl free solution can kill the cysts of C. stimuli and sterilize the breeding pool at the same time. , to prevent diseases and insect pests; 4. The dead cysts of Cryptonucleidium irritans adhering to the bottom of the culture pond can be completely removed by using the automatic rotating brush, and the dead cysts are directly discharged with the remaining seawater in the pond; It has no drug residues, no toxicity to fish, and no environmental pollution while efficiently killing the cysts of C. stimuli. It provides a healthy, environmentally friendly and efficient device for the killing of C. stimuli cysts in seawater aquaculture.

Description of drawings

FIG. 1 is a schematic structural composition diagram of an embodiment of the device for killing and stimulating Cysts of Cryptonariae by hydroxyl radicals according to the present invention.

Fig. 2 is the change of cyst morphology before and after the killing of hydroxyl radicals in the embodiment of the present invention. In Figure 2, A and C are pictures of cyst morphology in the control group; B and D are pictures of cyst morphology when TRO=5.27 mg/L.

Detailed ways

The present invention will be further described with reference to the accompanying drawings. However, the protection scope of the present invention is not limited to the embodiment.

Referring to Fig. 1, the device for rapidly killing and stimulating the cysts of Cryptonuclear worms by the hydroxyl radicals is provided with a water storage tank 1, a venturi gas-liquid mixer 5, a high-frequency high-voltage power supply 6, and a modular oxygen-active fragment generator 7. , On-line oxygen active debris detector 8, liquid-liquid mixer 9, TRO detector 10, motor 11, automatic rotating brush 12 and culture pool 13.

The water outlet of the water storage tank 1 is connected with the water inlet of the water pump 31, and a first valve 21 is arranged between the water storage tank 1 and the water pump 31; the water outlet of the water pump 31 is connected with the liquid inlet of the venturi gas-liquid mixer 5, and the water pump 31 is connected to A first flow meter 41 is arranged between the Venturi gas-liquid mixer 5; the air inlet of the modular oxygen active fragment generator 7 is connected to an external oxygen source, and a second flow meter 41 is arranged between the modular oxygen active fragment generator 7 and the oxygen source in sequence. The valve 22 and the second flow meter 42; the oxygen active debris outlet of the modular oxygen active debris generator 7 is sequentially connected to the online oxygen active debris detector 8 and the suction port of the Venturi gas-liquid mixer 5, and the online oxygen active debris A third valve 23 and a third flow meter 43 are arranged between the debris detector 8 and the Venturi gas-liquid mixer 5 in sequence; the liquid outlet of the Venturi gas-liquid mixer 5 and the liquid inlet of the liquid-liquid mixer 9 The outlet of the liquid-liquid mixer 9 is connected to the TRO detector 10 and the water inlet of the breeding tank 13 in sequence. The automatic rotating brush 12 controlled by the motor 11 is provided; the water outlet at the bottom of the culture pond 13 is externally connected to seawater, and the sixth outer valve 26 and the outer sampling valve 25 are arranged therebetween.

The following gives the method of hydroxyl radicals to kill and stimulate the cysts of Cryptonucleus:

1) From July to November every summer, when the water temperature is between 25 and 30 °C to stimulate the outbreak of cryptonium disease, when the fish feed decreases, the body rubs against the wall or bottom of the pool, the surface mucus increases, and there are obvious white spots, And some fry died; observe the white spots on the surface of the dead fish with a microscope, and find that they are black round or pear-shaped solid, which can confirm the occurrence of irritable cryptorhizobia. After a large number of cysts fell off from the diseased fish at 10:00 in the morning, all the diseased fish in the diseased breeding pond 13 were removed to other clean breeding ponds without disease, and the water level of the diseased breeding pond 13 was lowered. At this time, the bottom of the diseased breeding pond 13 was attached There is a large number of stimulatory Cryptonaria cysts. Turn on the high-frequency high-voltage power supply 6 to generate excitation voltage outside the modular oxygen active fragment generator 7, and open the valve 22 at the same time. For the gas containing oxygen active fragments, 1/15~1/10 of the gas containing oxygen active fragments enters the online oxygen active fragment detector 8 to detect the concentration of oxygen active fragments, and the remaining 9/10~14/15 enter the Venturi gas-liquid miscibility device 5. The power of the high-frequency high-voltage power supply is 150-300W, the intake air volume of oxygen is 1.0-5.0L/min; the gas flow rate of the oxygen active fragment concentration detector is 0.1-0.5mL/min, and the oxygen active fragment concentration It is 50～300mg/L.

2) Open the valve 21 and the water pump 31, so that the seawater stored in the storage tank 1 enters the Venturi gas-liquid mixer 5 after passing through the flow meter 41; the water flow rate is determined according to the number of cysts to be stimulated to stimulate Cryptonaria, and can be 10 to 50L /min; seawater and the gas containing oxygen-active fragments prepared in step 1) are fully mixed in the Venturi gas-liquid mixer 5 and liquid-liquid mixer 9 to generate a high-concentration hydroxyl radical solution, the concentration of hydroxyl radical solution It is regulated by water temperature, oxygen active fragment concentration, equilibrium partial pressure of oxygen-containing active fragment gas and gas-liquid volume ratio. L.

3) After the high-concentration hydroxyl radical solution generated in step 2) is injected into the culture tank 13, the water injection time is 1 h, the water flow is 10-50 L/min, and the water surface height is 0.1-0.5 m; Cysts of C. irritans at the bottom of the breeding tank 13 for 0.5 to 2 hours, and the death of C. irritans cysts was examined by microscopy.

4) After soaking in the hydroxyl radical solution to stimulate the Cysts of Cryptonariae to die in step 3), turn on the motor 11 to make the automatic rotating brush 12 rotate and work, and the brush rotates along the central axis at a speed of 10 r/min; rotate for 10 min Then, the motor 11 is turned off, and the valve 26 is opened, so that the dead cysts are discharged directly into the offshore waters along with the seawater.

The specific embodiment of killing and stimulating Cryptonaria cysts is given below:

Experiment on killing and stimulating Cysts of Cryptonucleus spp. with hydroxyl radical solution.

In mid-August, in the red snapper breeding pond of a breeding plant on Xiaodeng Island in Xiamen City, the fish fry had decreased feeding, rubbed the wall or bottom of the pond with their bodies, increased mucus on the body surface, obvious white spots, and some fry died. The water temperature of the breeding pond is 27-28℃, the salinity is 25.6‰, the pH is 7.75, and the dissolved oxygen is 6.78mg/L. Microscopic examination found that there were a large number of black, round or pear-shaped worms wrapped in the mucus on the surface of the diseased fish; and there were granular hard substances at the bottom of the breeding pond, and it was judged that it had irritated cryptorrhosis. Collected Cysts stimulated Cryptocaryon at the bottom of the pool for killing experiments, and characterized the killing of Cysts stimulated by Cryptonucleus under different ·OH doses. The number of cysts used in the experiment was 100 and the discharge power was 200W. The required concentration of hydroxyl radical solution was adjusted by adjusting the gas-liquid miscibility ratio. Four experimental groups were set up, namely the control group and three groups treated with different concentrations of hydroxyl radicals. The concentrations of the treatment groups were 1.32 mg/L, 2.63 mg/L and 5.27 mg/L. The hydroxyl radical solutions of different concentrations were directly poured into the beaker containing the cysts of C. stimuli, and after soaking for 2 h, the morphology of the cysts of C. stimuli before and after the killing of hydroxyl radicals was observed by microscope. The results are shown in Figure 2. In the control group, the outer shell of the cysts was complete, black pear-shaped or round, and an obvious split phase was observed in the cysts; the cysts were placed in clean seawater at a temperature of 25.2 °C for a period of time and found Hatching releases larvae. In contrast, the cysts of C. stimuli treated with hydroxyl radical solution with a concentration of 5.27 mg/L showed that the cyst walls were generally intact, but the color of the cysts was obviously lighter, and there was no split phase; the treated cysts were placed in It was found that the cysts could not continue to develop and release larvae in clean seawater at a temperature of 25.2 °C. Table 1 shows the killing effects of different concentrations of hydroxyl radical solutions on the cysts stimulated by Cryptonuclearia.

Table 1 The killing effect of different concentrations of hydroxyl radical solution on the cysts of Cryptonsis

The hydroxyl radical solution can oxidize the weak area of the cyst wall to cause holes to enter the cyst, and the substances and functional substances that make up the basic structure of the cell undergo irreversible reactions such as oxidative decomposition of amino acids, DNA chain breakage, etc., so that the cyst cannot be further developed. The larvae were released, and the remaining hydroxyl radicals were decomposed into O ₂ and H ₂ O, and the hydroxyl radical solution with a concentration of 5.27 mg/L could completely kill and stimulate the cysts of Cryptonucleus.

Claims (5)

1. a method for hydroxy radicals to kill and stimulate Cryptocystis cysts, it is characterized in that the device of hydroxy radicals to kill and stimulate Cryptocystis cysts is provided with water storage tank, cultivating pond, high frequency high voltage power supply, modular oxygen Active debris generator, online oxygen active debris detector, Venturi gas-liquid mixer, liquid-liquid mixer, TRO detector, motor, automatic rotating brush, water pump, valve, gas pipeline and liquid main pipeline; all The air inlet of the modular oxygen active debris generator is connected to an external oxygen source, and the oxygen active debris gas outlet of the modular oxygen active debris generator is sequentially connected to the online oxygen active debris detector and the suction port of the Venturi gas-liquid mixer; The water outlet of the storage tank is connected with the water inlet of the water pump, the water outlet of the water pump is connected with the liquid inlet of the Venturi gas-liquid mixer, and the liquid outlet of the Venturi gas-liquid mixer is connected with the liquid inlet of the liquid-liquid mixer, The liquid-liquid mixer outlet is connected to the TRO detector and the water inlet of the breeding tank in turn; an automatic rotating brush controlled by a motor is arranged in the middle of the breeding tank; seawater is connected to the water outlet at the bottom of the breeding tank; the oxygen source is connected to the modular oxygen source. An oxygen valve and a gas flow meter are arranged between the active debris generators in sequence; an oxygen-containing active debris gas valve and an oxygen-containing active debris gas flow rate are arranged between the online oxygen active debris detector and the venturi gas-liquid mixer in sequence. There is a main pipeline liquid valve between the water storage tank and the water pump, a liquid flow meter is arranged between the water pump and the Venturi gas-liquid mixer; an external discharge liquid valve is arranged between the culture tank and the external seawater; Between the TRO detector and the culturing pond, a sampling valve of the main water inlet is set, and between the culturing pond and the external seawater, an efflux sampling valve is set; The method includes the following steps: 1) From July to November every year, when the water temperature is at 25 to 30 °C to stimulate the outbreak of cryptonium disease, if the fish feed decreases, the body rubs against the wall or bottom of the pool, the surface mucus increases, and there are obvious white spots, and Some fry died; the white spots on the surface of the dead fish were observed with a microscope, and it was found to be black round or pear-shaped, which confirmed the presence of irritable cryptorhizobia; at 10:00 in the morning, a large number of cysts emerged from the diseased fish. After falling off, remove all the diseased fish in the diseased breeding pond into other clean breeding ponds without disease to reduce the water level in the diseased breeding pond. There is a water inlet above the side of the diseased breeding pond, the bottom of the pond is a horizontal and smooth bottom, and there is an automatic rotating brush controlled by a motor in the center of the pond. The length of the brush plate is 3.8m and the width is 0.3m; the brush plate is made of polypropylene smooth brush plate, and the brush plate is covered with nylon wire bristles with a length of 0.15m; after the motor is started, the automatic rotating brush rotates along the The stainless steel tube of the central axis rotates at a speed of 10r/min, and there is a drain at the bottom of the side wall of the pool; 2) Turn on the switch of the modular oxygen active fragment generator, open the oxygen valve to introduce oxygen, and start the high-frequency high-voltage power supply, so that the oxygen passing through the modular oxygen active fragment generator is rapidly ionized and dissociated into gaseous oxygen active fragments; open the oxygen-containing active fragment Fragment valve, after the gaseous oxygen active fragments are detected by the online oxygen active fragment concentration detector, they enter the Venturi gas-liquid mixer; 3) open the water pump and the main pipeline liquid valve, and store the clean seawater in the storage tank and inject the water pump into the venturi gas-liquid miscible through the pipeline; the gaseous oxygen active fragments and seawater entering the venturi gas-liquid miscible in the described step 2) Fully miscible in the Venturi gas-liquid mixer and liquid-liquid mixer to generate a high-concentration hydroxyl radical solution, which passes through the TRO detector through the pipeline and enters the diseased breeding tank; the flow rate of the seawater is 10-50L/ min, the total oxidant TRO concentration of the hydroxyl radical solution is 2～30mg/L; 4) After the hydroxyl radical solution is injected into the diseased breeding pond, soak the bottom of the diseased breeding pond for 0.5 to 2.0 hours to stimulate the cysts of Cryptonuclear worms, and the hydroxyl radical solution oxidizes the weak area of the cyst wall to make holes appear and enter the inside of the cyst. Substances and functional substances in the basic structure of cells undergo irreversible reactions, including oxidative decomposition of amino acids and DNA strand breaks, so that the cysts die and cannot develop further to release larvae, and the remaining hydroxyl radicals are decomposed into O ₂ and H ₂ O; disease breeding There is an automatic rotating brush controlled by a motor in the middle of the pond, which automatically rotates and cleans the cysts at the bottom of the pond after starting; after 10 minutes of rotation, the efflux liquid valve is opened to discharge the dead cysts in the diseased breeding pond with the seawater; The cysts were immersed in the hydroxyl radical solution for 0.5-2.0 hours, and after the cysts were confirmed dead by microscope inspection, the automatic rotary brush switch was activated. 2. a kind of hydroxyl radical as claimed in claim 1 kills and stimulates the method for Cryptocaryon cyst, it is characterized in that in step 1), described disease culturing pond is class cylinder open type cement pond, and radius is 2m , with a depth of 1.5m and a total volume of 20m ³ . 3. a kind of hydroxyl radical kills and stimulates the method for the cyst of Cryptonaria as claimed in claim 1, it is characterized in that in step 2) in, the power of described high-frequency high-voltage power supply is 150～300W, and oxygen intake The oxygen is ionized and dissociated after being bombarded by high-energy electrons to form gaseous oxygen active fragments. 4. as claimed in claim 3, a kind of hydroxyl radical kills and stimulates the method for cyst of Cryptocaryon worm, it is characterized in that described gaseous oxygen active fragment comprises O ₂ ⁺ , O ( ¹ D), O ₂ (a ¹ g ), O ₂ ^- , O( ³ P) and O ₃ particles, and the concentration reaches 50-300 mg/L; the pipeline is a polytetrafluoroethylene tube. 5. as claimed in claim 1, a kind of hydroxyl radical kills and stimulates the method for Cyst of Cryptocartonia, it is characterized in that in step 3) in, the water storage tank is a cubic closed cement tank, and the total volume is 20m ³ , so The stored seawater is clean seawater that has been pretreated by sand filtration.

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