CN114600804A - Culture treatment system for egg-shaped pompano - Google Patents
Culture treatment system for egg-shaped pompano Download PDFInfo
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- 238000011282 treatment Methods 0.000 title claims abstract description 153
- 241000276694 Carangidae Species 0.000 title claims description 16
- 241000719209 Trachinotus ovatus Species 0.000 claims abstract description 118
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 87
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- 230000005415 magnetization Effects 0.000 claims abstract description 26
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- 238000012545 processing Methods 0.000 claims description 30
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- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 235000019750 Crude protein Nutrition 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 6
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- 238000012258 culturing Methods 0.000 claims description 3
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 claims description 2
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- 238000012360 testing method Methods 0.000 abstract description 15
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- 206010013975 Dyspnoeas Diseases 0.000 description 1
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- 206010027982 Morphoea Diseases 0.000 description 1
- 241000277275 Oncorhynchus mykiss Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 241000719226 Trachinotus Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
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- 229960000686 benzalkonium chloride Drugs 0.000 description 1
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
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- 239000013505 freshwater Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
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- 230000028993 immune response Effects 0.000 description 1
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- 235000013372 meat Nutrition 0.000 description 1
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- 210000003097 mucus Anatomy 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 230000001706 oxygenating effect Effects 0.000 description 1
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- 239000012286 potassium permanganate Substances 0.000 description 1
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- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
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- JNMRHUJNCSQMMB-UHFFFAOYSA-N sulfathiazole Chemical compound C1=CC(N)=CC=C1S(=O)(=O)NC1=NC=CS1 JNMRHUJNCSQMMB-UHFFFAOYSA-N 0.000 description 1
- 229960001544 sulfathiazole Drugs 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The culture treatment system for the trachinotus ovatus capable of preventing and treating cryptocaryon irritans is established for the first time, a plurality of effective physical and chemical treatment means are screened and optimized, and an effective defense effect is achieved for the cryptocaryon irritans to infect the trachinotus ovatus. The culture treatment system for the trachinotus ovatus comprises an acetic acid treatment tank, a starvation treatment area, an ultraviolet light treatment area, a dark treatment area, a magnetization treatment area, an oxygenation treatment area, an ultrasonic treatment area, an orange green light treatment area and a nutrition strengthening tank which are sequentially arranged, and the culture treatment system obtains good effects no matter in a test research stage or a production practice stage.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to a culture treatment system capable of effectively improving the survival rate of trachinotus ovatus.
Background
Trachinotus ovatus, trachinotus, golden pompano, yellow wax pompano, etc. are popular in southeast Asia market because of their tender meat, delicious taste and rich nutrition. The trachinotus ovatus has the characteristics of high growth speed, strong environment adaptability, high survival rate and the like, is generally cultivated in the same year, can reach the specification of commercial products in the same year, has obvious economic benefit, and is popular among cultivation manufacturers. With the development of artificial breeding and culture technology, trachinotus ovatus becomes an economic fish widely cultured in southern coastal areas in China. The culture modes of the trachinotus ovatus mainly comprise net cage culture, pond culture and the like. In recent years, the demand of trachinotus ovatus in domestic markets is rising, and the trachinotus ovatus is also concerned in international markets, particularly in the states, japan, korea and the major aquatic product consuming countries of the european union, and is a bright spot variety for the export of aquatic products. The development prospect of the culture of the pompano is wide in the face of the demands of domestic and international markets for the pompano. However, with the rapid increase of the culture yield of the trachinotus ovatus, the increase of the culture density, the continuous deterioration of the culture environment and the frequent outbreak of the trachinotus ovatus diseases, the development of the marine culture industry in China is seriously disturbed. In recent years, frequent outbreak of white spot disease of trachinotus ovatus along the coast in south China is more and more extensive, becomes a main epidemic disease for the cultivation of marine fishes in Fujian, Zhejiang, Guangdong, Guangxi and Hainan, and causes great economic loss to the marine aquaculture industry in China.
Cryptocaryon irritans disease is also called as 'marine ichthyophthiriasis', is a main disease of marine cultured fishes, mainly occurs in tropical and subtropical marine culture areas, and reports of cryptocaryon irritans outbreak are reported from time to time in Australia, New Zealand, southern United states, Kowitt, Thailand, Singapore, Malaysia, Taiwan in China, and coastal areas in southern continental. Its etiology stimulates cryptocaryon irritans (Cryptocaryon irritans Brown 1951) Is a ciliate parasite in the gill and skin cuticle of marine teleostean. The clinical symptoms of the disease are: white spots, mucus increase, dyspnea and anorexia appear on the body surface and gills, and diseased fishes show behavior characteristics of easy fright, quick swimming, swimming against water flow and the like. Some of the identifying characteristics of cryptocaryon irritans are described in detail by Lom and Dykova (1992), and the method for determining cryptocaryon irritans disease is: the fish has continuously rotating elliptic ciliates in the gills, skin or fin rays, and white spots are observed visually.
At present, the method for preventing and treating the seawater ichthyophthiriasis mainly comprises four types, the first type is a physical method which mainly comprises heat treatment, ultraviolet ray treatment, ozone treatment and the like, and the methods play a certain role in the process of preventing and treating the seawater ichthyophthiriasis, but have limited effects. Secondly, an ecological method is also explored by foreigners about the ecological prevention and control of cryptocaryon irritans, such as drying treatment, fresh water fish body soaking, alternate culture and the like. And thirdly, chemical prevention and treatment, wherein chemical prevention and treatment measures such as sodium hypochlorite, benzalkonium chloride, copper sulfate, formalin, potassium permanganate, sulfathiazole and the like are researched for soaking medicaments, but the chemical treatment measures also have certain defects, for example, some medicaments have side effects on fish bodies, and some curative effects are not very exact. Fourthly, an immunological method is adopted, in 1942, Duff feeds the oncorhynchus mykiss with inactivated aeromonas salmonicida for the first time, so that the host fish obtains stronger immune protection capability, and a milestone for research and application of fish vaccines is established; crowing spring et al (2007) found that intraperitoneal injection or body surface infection stimulated cryptocaryon larvae caused a protective immune response to the parasite in Epinephelus coioides, and antibodies were detected in both serum and skin culture supernatants of immunized fish; however, the current vaccine research on the seawater ichthyophthiriasis is still in the laboratory stage, and no stable and reliable vaccine is available on the market. Therefore, in the culture process of the trachinotus ovatus, a culture treatment system capable of effectively resisting cryptocaryon irritans is urgently needed.
Disclosure of Invention
The invention aims to solve the problem of difficulty in preventing cryptocaryon irritans in the culture process of trachinotus ovatus, and provides a culture treatment system for trachinotus ovatus, which can be used for sequentially treating trachinotus ovatus in a flow manner, is convenient to operate, safe and effective, has small side effect on trachinotus ovatus, and has a good effect on preventing cryptocaryon irritans. The method is not only suitable for trachinotus ovatus infected with cryptocaryon irritans or suspected cryptocaryon irritans, but also suitable for non-diseased trachinotus ovatus to improve the disease resistance.
The culture treatment system for the trachinotus ovatus capable of preventing and treating cryptocaryon irritans is established for the first time, various effective physical and chemical treatment means are screened and optimized, an effective resisting effect is achieved for the cryptocaryon irritans to infect the trachinotus ovatus, and the culture treatment system achieves good effects no matter in the experimental research stage or the production practice stage.
The invention is realized by the following technical scheme:
the utility model provides a processing system is bred to oval pompano, is including acetic acid processing pond, hunger processing area, ultraviolet ray processing area, dark processing area, magnetization processing area, oxygenating processing area, ultrasonic treatment district, orange green light processing area, the nutrition intensification pond that arranges in proper order, the acetic acid processing pond is located the foremost end of breeding processing system, and the nutrition intensification pond is located the rearmost end of breeding processing system, hunger processing area, ultraviolet ray processing area are handledThe area, the dark processing area, the magnetization processing area, the oxygenation processing area, the ultrasonic processing area and the orange green light processing area are all closed culture rooms, the culture rooms can be communicated with one another and are separated from one another through a gate, a driving net, a driving motor and a control switch are arranged in each culture room, the control switch can open the driving motor, and then the driving net is driven to drive the oval pompano to the next culture room. The areas of the acetic acid treatment tank, the starvation treatment area, the ultraviolet light treatment area, the dark treatment area, the magnetization treatment area, the oxygenation treatment area, the ultrasonic treatment area, the orange green light treatment area and the nutrition strengthening tank are all 10m2About 1m in water depth, wherein the mass concentration in the acetic acid treatment tank is 80-100ml/m3The acetic acid water solution is characterized in that a positive electrode plate and a negative electrode plate are arranged in an acetic acid treatment pool, the voltage for electrifying the positive electrode plate and the negative electrode plate is 2-2.5V, the current is 15-18mA, an ultraviolet germicidal lamp is arranged in an ultraviolet light treatment area, the generated wavelength is 320-350 nm, the dark treatment area is completely light-proof, a magnetization switch is arranged in the magnetization treatment area, after the magnetization switch is opened, a variable electromagnetic field is introduced into the magnetization treatment area, the introduction time of the electromagnetic field is 1.5h, the intensity of the electromagnetic field is weakened from weak to strong and is respectively 230-. An oxygenation pump is arranged in the oxygenation treatment area, and the dissolved oxygen in the oxygenation treatment area reaches more than 10 mg/L. An ultrasonic generator is arranged in the ultrasonic processing area, the power of the generated ultrasonic is 1000W, and the frequency is 20-22 kHz. The orange green light processing area is provided with an orange light lamp, a green light lamp and a switch, after the switch is turned on, the orange light lamp is turned off after being turned on for 10min, the green light lamp is turned off after being turned on for 10min, the wavelength generated by the orange light lamp is 600-610 nm, the illumination intensity is 1800-2000 lx, the wavelength generated by the green light lamp is 550-560 nm, and the illumination intensity is 1000-1500 lx.
The application method of the trachinotus ovatus culture treatment system comprises the following steps: placing the oval pompano into an acetic acid treatment pool, wherein the mass concentration is 80-100ml/m3Soaking in acetic acid aqueous solution for 15min, and applying weak current with voltage of 2-2.5V, current of 15-18mA and energization time of 2-2.5V and 15-18mAAfter 20min, the trachinotus ovatus is transferred into a starvation treatment area, after 5-6 hours of starvation treatment, a gate and a control switch of the starvation treatment area are opened in sequence, and a driving motor drives a driving net to drive the trachinotus ovatus to the ultraviolet light treatment area; after being treated by ultraviolet light of 320-350 nm for 10min, the gate and the control switch of the ultraviolet light treatment area are opened in sequence, and the driving motor drives the driving net to drive the trachinotus ovatus to the dark treatment area; after 3 hours of dark treatment (namely, allowing the fishes to stand in the dark treatment area), opening a gate and a control switch of the dark treatment area, and driving a driving net by a driving motor to drive the oval pompano to a magnetization treatment area; opening the magnetization switch, sequentially introducing electromagnetic fields of 230-; the dissolved oxygen of the oxygenation treatment area reaches a supersaturation level, the dissolved oxygen reaches more than 10mg/L, after the trachinotus ovatus stays in the oxygenation treatment area for 30min, a gate and a control switch of the oxygenation treatment area are opened in sequence, and a driving motor drives a driving net to drive the trachinotus ovatus to the ultrasonic treatment area; after ultrasonic treatment for 5min with the power of 1000W and the frequency of 20-22kHz, opening a gate and a control switch of an ultrasonic treatment area in sequence, and driving a driving net by a driving motor to drive the trachinotus ovatus to an orange green light treatment area; opening a switch in the orange green light processing area, opening a gate and a control switch of the orange green light processing area after the orange light lamp and the green light lamp are respectively lighted for 10min, and driving a driving net to drive the oval pompano to a nutrition strengthening pool by a driving motor; temporarily culturing trachinotus ovatus in a nutrition-enhanced pond for three days, and feeding for 5 times every day: 7:00, feeding a mixed solution of rotifers and artemia, wherein the feeding density is 18-20/mL; 10:00, feeding the complete compound feed for the marine fish with the crude protein content of more than 40 percent, wherein the feeding amount is 18-20 percent of the weight of the fish; 13:00, feeding the minced fish and the shrimp, wherein the feeding amount is 8-10% of the weight of the fish; feeding mixed liquid of the daphnia and the chlorella at a ratio of 16:00, wherein the feeding density is 80-100 per mL; 19:00, feeding expanded feed with the particle size of 2 mm and the crude protein content of less than 30 percent, wherein the feeding amount is 10-13 percent of the weight of the fish; after three days of nutrition enrichment, the trachinotus ovatus can be transported out of the culture treatment system for normal culture.
The culture treatment system for the trachinotus ovatus can be used for the trachinotus ovatus infected with cryptocaryon irritans or suspected to be infected with cryptocaryon irritans, and can also be used for the trachinotus ovatus which is not diseased to prevent the infection of the cryptocaryon irritans.
The invention has the advantages and effects that: the trachinotus ovatus culture treatment system is used for preventing and treating cryptocaryon irritans for the first time, various treatment means are screened and optimized to carry out flow treatment on the trachinotus ovatus, the operation is easy, no adverse effect is caused on the trachinotus ovatus, and the immunity and the disease resistance of the trachinotus ovatus to cryptocaryon irritans can be effectively improved.
The acetic acid aqueous solution is weakly acidic, not only can sterilize the bodies of the fishes, but also can enhance the activity of the trachinotus ovatus, stimulate the trachinotus ovatus to get rid of cryptocaryon irritans on the gill parts and the skin, and meanwhile, a weak current is introduced into the water body by virtue of the electrode plate, so that electrons in the acetic acid solution flow, further stimulate the trachinotus ovatus to get rid of the cryptocaryon irritans, and improve the disease resistance of the trachinotus ovatus.
The starvation treatment can reduce the stress response of the subsequent treatment program to the trachinotus ovatus, the final nutrition strengthening can effectively supplement the energy consumption of the trachinotus ovatus in the treatment process, different baits are purposefully fed in five different time periods every day, the nutrition is effectively supplemented, and the immunity and the disease resistance of the trachinotus ovatus are improved.
The vitality and immunity of the trachinotus ovatus can be enhanced by ultraviolet light treatment, dark treatment, magnetization treatment, oxygen supersaturation treatment, ultrasonic treatment and orange green light treatment, the vitality and the immunity of the trachinotus ovatus are improved by combining the treatment technologies, and the treatment parameters are optimized, so that the vitality and the disease resistance of the trachinotus ovatus are improved, the cryptocaryon irritans can be stimulated to separate from fish bodies, and the trachinotus ovatus can be free from the infection of the cryptocaryon irritans.
The ultrasonic waves and the electromagnetic field have medical biological effects, the ultrasonic waves with certain frequency and the electromagnetic field radiation with certain intensity can enhance the activity of enzymes in fish and shrimp bodies, improve the hatching of fertilized eggs of the fish and shrimp, promote the growth and development of seedlings and the like, after the ultrasonic waves with proper light intensity and the electromagnetic field radiation with proper light intensity can improve the growth speed of the fish and shrimp and enhance the immunologic function of the fish and shrimp, and the ultrasonic waves with high intensity and the electromagnetic field radiation with high intensity can break biological cells and reduce the activity of the enzymes and the like. Ultrasonic waves with different frequencies and electromagnetic field radiation with different intensities can have certain influence on the digestibility of the young fish and shrimp, which is probably because the low-frequency ultrasonic waves and the low-intensity electromagnetic field radiation can change the conformation of some digestive enzymes in the bodies of the fish and shrimp to change the catalytic activity of the digestive enzymes, thereby changing the combination degree of enzyme molecules and substrates to influence the digestion of the young fish and shrimp. The ultrasonic treatment area and the magnetization treatment area optimally screen the ultrasonic frequency and the electromagnetic field intensity suitable for the trachinotus ovatus, so that the growth speed of the trachinotus ovatus can be improved, the immune function of the trachinotus ovatus can be enhanced, and cryptocaryon irritans can be stimulated to separate from the trachinotus ovatus body.
The orange light and the green light can influence the enzyme activity in the fish body to a certain extent, can influence the growth and development of the fish body, the orange green light with proper wavelength and light intensity can improve the growth speed of the trachinotus ovatus and improve the disease resistance of the fish body, and simultaneously the orange light and the green light can also generate bad stimulation to cryptocaryon irritans, so that the cryptocaryon irritans irregularly moves and falls off from the body surface of the host. The orange-green light processing area optimally screens the orange light wavelength light intensity and the green light wavelength light intensity suitable for the trachinotus ovatus, thereby being beneficial to the improvement of the disease resistance of the trachinotus ovatus and the stimulation of cryptocaryon to be separated from the trachinotus ovatus body. Detailed Description
EXAMPLE one optimization of acetic acid concentration and electrode plate energization time in an acetic acid treatment tank
Setting the concentration of five groups of acetic acid: 40ml/m3、60ml/m3、80ml/m3、100ml/m3、120ml/m3Soaking trachinotus ovatus infected with cryptocaryon irritans for 15min, counting cryptocaryon irritans at the gill part of trachinotus ovatus before and after soaking, and observing stress reaction of trachinotus ovatus. The test result shows that the concentration is 40ml/m3、60ml/m3、80ml/m3、100ml/m3、120ml/m3After the carangid is soaked in the acetic acid solution, cryptocaryon irritans at the gill part of the trachinotus ovatus are respectively reduced by 11%, 19%, 35%, 36% and 38%. Trachinotus ovatus 40ml/m3、60ml/m3、80ml/m3、100ml/m3Has no stress response to the concentration of 120ml/m3The concentration of (a) shows a stress response. Therefore, the concentration of the acetic acid in the acetic acid treatment pool is selected to be 80-100ml/m3。
The mass concentration is 80-100ml/m3Inserting an electrode plate into the acetic acid aqueous solution, electrifying weak current, wherein the voltage is 2-2.5V, the current is 15-18mA, setting six test groups and a blank control group, wherein the electrifying time of the six test groups is respectively 5min, 10min, 15min, 20min, 25min and 30min, the blank control group is not electrified, placing the trachinotus ovatus with the corresponding cryptocaryon irritans infection amount into the seven groups, counting the cryptocaryon irritans at the branchia part of the trachinotus ovatus before and after treatment, and observing the stress reaction of the trachinotus ovatus. Test results show that the cryptocaryon irritans at the gill part of the trachinotus ovatus are respectively reduced by 8%, 15%, 21%, 35%, 36% and 37% by electrifying for 5min, 10min, 15min, 20min, 25min and 30 min. The number of cryptocaryon irritans in the blank control group did not change. The electrified trachinotus ovatus has no stress reaction for 5min, 10min, 15min and 20min, and the electrified trachinotus ovatus has stress reaction for 25min and 30 min. Therefore, the power-on time is selected to be 20 min.
EXAMPLE two optimization of UV treatment time
And (3) respectively treating the trachinotus ovatus infected with cryptocaryon irritans for 0min, 5min, 10min, 15min and 20min by using ultraviolet light with the wavelength of 320-350 nm, counting the cryptocaryon irritans at the branchia part of the trachinotus ovatus before and after treatment, and observing the stress response of the trachinotus ovatus. Test results show that cryptocaryon irritans at the branchia of trachinotus ovatus are reduced by 0%, 8%, 26%, 27% and 28% respectively by treatment for 0min, 5min, 10min, 15min and 20 min. Stress reaction does not occur in the trachinotus ovatus treated for 0min, 5min and 10min, and stress reaction occurs in the trachinotus ovatus treated for 15min and 20min after electrification. Thus, the UV light was selected for 10 min.
EXAMPLE III optimization of the electromagnetic field intensity in the magnetization treatment zone
Four test groups are arranged, the first group of electromagnetic field strength is 230-250mT, the second group of electromagnetic field strength is 730-750mT, the third group of electromagnetic field strength is 430-450mT, the fourth group of electromagnetic field is a variable electromagnetic field which is changed from weak to strong and then becomes weak, and the blank control group is not accessed with the electromagnetic field. Respectively treating Trachinotus ovatus infected with cryptocaryon irritans for 1.5h, and respectively introducing electromagnetic fields of 230-. Test results show that the fourth group of electromagnetic field treatment has the largest influence on cryptocaryon irritans, so that the cryptocaryon irritans at the branchia part of the trachinotus ovatus is reduced by 41%. The number of cryptocaryon irritans in the blank control group did not change. No obvious stress reaction occurs in the four test groups of trachinotus ovatus.
EXAMPLE four optimization of ultrasonic frequency in sonication zone
Respectively treating Trachinotus ovatus infected with Cryptocaryon irritans for 5min by using ultrasonic waves with frequencies of 20-22kHz, 30-32kHz, 40-42kHz, 50-52kHz and 60-62kHz, respectively counting the Cryptocaryon irritans at the gill part of Trachinotus ovatus before and after treatment, and observing the stress response of Trachinotus ovatus. Test results show that the ultrasonic treatment with the frequency of 20-22kHz has the largest influence on cryptocaryon irritans, so that the cryptocaryon irritans at the gill part of the trachinotus ovatus is reduced by 36%. No obvious stress reaction occurs in the trachinotus ovatus in five groups of experiments.
EXAMPLE V optimization of treatment time in orange-Green treatment zone
Five test groups are set, the first group is irradiated by an orange light for 10min, the second group is irradiated by the orange light for 20min, the third group is irradiated by a green light for 10min, the fourth group is irradiated by the green light for 20min, the fifth group is irradiated by the orange light and the green light for 10min respectively, the blank control group is not irradiated, the wavelength of the orange light is 600610 nm, the illumination intensity is 1800-2000 lx, the wavelength of the green light is 550560 nm, and the illumination intensity is 1000-1500 lx. Before and after treatment, cryptocaryon irritans at the gill part of the trachinotus ovatus are counted, and the stress response of the trachinotus ovatus is observed. The test result shows that the fifth group of treatments has the largest influence on cryptocaryon irritans, so that the cryptocaryon irritans at the branchia part of the trachinotus ovatus is reduced by 37%. The number of cryptocaryon irritans in the blank control group did not change. No obvious stress reaction occurs in five test groups of trachinotus ovatus.
EXAMPLE VI optimization of nutrient enrichment
The control group was fed with conventional diet 3 times a day. Two test groups are provided, wherein the first group feeds the bait 5 times a day and feeds conventional bait; the second group was fed 5 times per day: 7:00, feeding a mixed solution of rotifers and artemia, wherein the feeding density is 18-20/mL; 10:00, feeding the complete compound feed for the marine fish with the crude protein content of more than 40 percent, wherein the feeding amount is 18-20 percent of the weight of the fish; 13:00, feeding the minced fish and the shrimp, wherein the feeding amount is 8-10% of the weight of the fish; feeding mixed liquid of the daphnia and the chlorella at a ratio of 16:00, wherein the feeding density is 80-100 per mL; 19:00, feeding expanded feed with the particle size of 2 mm and the crude protein content of less than 30 percent, wherein the feeding amount is 10-13 percent of the weight of the fish. The feeding time is 3 days, and the fed object is trachinotus ovatus which is infected with cryptocaryon irritans and is sequentially subjected to acetic acid treatment, hunger treatment, ultraviolet light treatment, dark treatment, magnetization treatment, oxygenation treatment, ultrasonic treatment and orange green light treatment. And measuring the weight change of the trachinotus ovatus before and after feeding. Test results show that the second group of feeding modes has the largest influence on the fish body weight, so that the fish body weight is increased by 7%. The control group increased by 1.5% and the first group increased by 2.5%. And the vitality of the trachinotus ovatus fed by the second group is obviously better than that of the trachinotus ovatus fed by the other two groups.
Example application of culture treatment system for trachinotus ovatus
Placing the trachinotus ovatus infected with cryptocaryon irritans into an acetic acid treatment pool, and using the mass concentration of 80-100ml/m3Soaking in acetic acid aqueous solution for 15min, applying weak current, voltage of 2-2.5V, current of 15-18mA, and electrifying for 20min to the negative electrode plate and the positive electrode plate, transferring the trachinotus ovatus to a starvation treatment area, opening a gate and a control switch of the starvation treatment area after starvation treatment for 5-6 hr, and driving a driving net to drive the trachinotus ovatus to the ultraviolet light treatment area by a driving motor; after being treated by ultraviolet light of 320-350 nm for 10min, the gate and the control switch of the ultraviolet light treatment area are opened in sequence, and the driving motor drives the driving net to drive the trachinotus ovatus to the dark treatment area; after 3 hours of dark treatment (namely, the fish is stood in the dark treatment area), the gate and the control switch of the dark treatment area are opened, and the driving motor drives the driving net to drive the oval pompano to the magnetization treatment area(ii) a Opening the magnetization switch, sequentially introducing electromagnetic fields of 230-; the dissolved oxygen in the oxygenation treatment area reaches the supersaturation level, the dissolved oxygen reaches more than 10mg/L, after the trachinotus ovatus stays in the oxygenation treatment area for 30min, a gate and a control switch of the oxygenation treatment area are opened sequentially, and a driving motor drives a driving net to drive the trachinotus ovatus to the ultrasonic treatment area; after ultrasonic treatment for 5min with the power of 1000W and the frequency of 20-22kHz, opening a gate and a control switch of an ultrasonic treatment area in sequence, and driving a driving net by a driving motor to drive the trachinotus ovatus to an orange green light treatment area; opening a switch in the orange green light processing area, opening a gate and a control switch of the orange green light processing area after the orange light lamp and the green light lamp are sequentially lightened for 10min, and driving a driving net to drive the oval pompano to a nutrition strengthening pool by a driving motor; temporarily culturing trachinotus ovatus in a nutrition-enhanced pond for three days, and feeding 5 times every day: 7:00, feeding a mixed solution of rotifers and artemia, wherein the feeding density is 18-20/mL; 10:00, feeding the complete compound feed for the marine fish with the crude protein content of more than 40 percent, wherein the feeding amount is 18-20 percent of the weight of the fish; 13:00, feeding the minced fish and the shrimp, wherein the feeding amount is 8-10% of the weight of the fish; feeding mixed liquid of the daphnia and the chlorella at a ratio of 16:00, wherein the feeding density is 80-100 per mL; 19:00, feeding expanded feed with the particle size of 2 mm and the crude protein content of less than 30 percent, wherein the feeding amount is 10-13 percent of the weight of the fish; and after three days of nutrition enrichment, transporting the trachinotus ovatus out of the culture treatment system, and detecting the cryptocaryon irritans infection on the body surface and the branchia part of the trachinotus ovatus, wherein the cryptocaryon irritans residue is not detected, namely the cryptocaryon irritans completely fall off.
Comparative examples 1 to 9: comparative examples 1 to 9 were compared with example seven, in which one treatment zone (acetic acid treatment tank, starvation treatment zone, ultraviolet light treatment zone, dark treatment zone, magnetization treatment zone, oxygenation treatment zone, ultrasonic treatment zone, orange-green light treatment zone, nutrient enrichment tank) was reduced, and other compositions and treatment methods were the same as example seven. Finally, the infection condition of the treated trachinotus ovatus is detected, and a small amount of cryptocaryon irritans residues are detected in the comparative examples 1 to 9, namely, the cryptocaryon irritans do not completely fall off.
Claims (7)
1. A kind of oval pompano breeds the processing system, characterized by that: the cultivation system comprises an acetic acid treatment pool, a hunger treatment area, an ultraviolet light treatment area, a dark treatment area, a magnetization treatment area, an oxygenation treatment area, an ultrasonic treatment area, an orange green light treatment area and a nutrition strengthening pool which are sequentially arranged, wherein the acetic acid treatment pool is positioned at the foremost end of a cultivation treatment system, the nutrition strengthening pool is positioned at the rearmost end of the cultivation treatment system, the hunger treatment area, the ultraviolet light treatment area, the dark treatment area, the magnetization treatment area, the oxygenation treatment area, the ultrasonic treatment area and the orange green light treatment area are all closed cultivation rooms, the cultivation rooms can be mutually communicated and are mutually separated through a gate, a driving net, a driving motor and a control switch are arranged in each cultivation room, the control switch can open the driving motor, and then the driving net is driven to drive the trachinotus ovatus to the next cultivation room; the areas of the acetic acid treatment tank, the starvation treatment area, the ultraviolet light treatment area, the dark treatment area, the magnetization treatment area, the oxygenation treatment area, the ultrasonic treatment area, the orange green light treatment area and the nutrition strengthening tank are all 10m2About 1m in depth of water, wherein the mass concentration in the acetic acid treatment tank is 80-100ml/m3The acetic acid water solution is characterized in that a positive electrode plate and a negative electrode plate are arranged in an acetic acid treatment pool, the voltage for electrifying the positive electrode plate and the negative electrode plate is 2-2.5V, the current is 15-18mA, an ultraviolet germicidal lamp is arranged in an ultraviolet light treatment area, the generated wavelength is 320-350 nm, the dark treatment area is completely light-proof, a magnetization switch is arranged in the magnetization treatment area, after the magnetization switch is opened, a variable electromagnetic field is introduced into the magnetization treatment area, the introduction time of the electromagnetic field is 1.5h, the intensity of the electromagnetic field is weakened from weak to strong and is respectively 230-.
2. An oxygenation pump is arranged in the oxygenation treatment area, and the dissolved oxygen in the oxygenation treatment area reaches more than 10 mg/L.
3. An ultrasonic generator is arranged in the ultrasonic processing area, the power of the generated ultrasonic is 1000W, and the frequency is 20-22 kHz.
4. The orange green light processing area is provided with an orange light lamp, a green light lamp and a switch, after the switch is turned on, the orange light lamp is turned off after being turned on for 10min, the green light lamp is turned off after being turned on for 10min, the wavelength generated by the orange light lamp is 600-610 nm, the illumination intensity is 1800-2000 lx, the wavelength generated by the green light lamp is 550-560 nm, and the illumination intensity is 1000-1500 lx.
5. The application method of the trachinotus ovatus culture treatment system comprises the following steps: placing the trachinotus ovatus into an acetic acid treatment pool, wherein the mass concentration is 80-100ml/m3Soaking in acetic acid aqueous solution for 15min, applying weak current, voltage of 2-2.5V, current of 15-18mA, and electrifying for 20min to the negative electrode plate and the positive electrode plate, transferring the trachinotus ovatus to a starvation treatment area, opening a gate and a control switch of the starvation treatment area after starvation treatment for 5-6 hr, and driving a driving net to drive the trachinotus ovatus to the ultraviolet light treatment area by a driving motor; after being treated by ultraviolet light of 320-350 nm for 10min, the gate and the control switch of the ultraviolet light treatment area are opened in sequence, and the driving motor drives the driving net to drive the trachinotus ovatus to the dark treatment area; after 3 hours of dark treatment, opening a gate and a control switch of the dark treatment area in sequence, and driving a driving net to drive the oval pompano to the magnetization treatment area by a driving motor; opening the magnetization switch, sequentially introducing electromagnetic fields of 230-; the dissolved oxygen in the oxygenation treatment area reaches the supersaturation level, the dissolved oxygen reaches more than 10mg/L, after the trachinotus ovatus stays in the oxygenation treatment area for 30min, a gate and a control switch of the oxygenation treatment area are opened sequentially, and a driving motor drives a driving net to drive the trachinotus ovatus to the ultrasonic treatment area; after ultrasonic treatment with power of 1000W and frequency of 20-22kHz for 5min, the gate of the ultrasonic treatment area is opened successivelyAnd a control switch, wherein the driving motor drives the driving net to drive the trachinotus ovatus to the orange green light treatment area; opening a switch in the orange green light processing area, opening a gate and a control switch of the orange green light processing area after the orange light lamp and the green light lamp are respectively lighted for 10min, and driving a driving net to drive the oval pompano to a nutrition strengthening pool by a driving motor; temporarily culturing trachinotus ovatus in a nutrition-enhanced pond for three days, and feeding 5 times every day: 7:00, feeding a mixed solution of rotifers and artemia, wherein the feeding density is 18-20/mL; 10:00, feeding the complete compound feed for the marine fish with the crude protein content of more than 40 percent, wherein the feeding amount is 18-20 percent of the weight of the fish; 13:00, feeding the minced fish and the shrimp, wherein the feeding amount is 8-10% of the weight of the fish; feeding mixed liquid of the daphnia and the chlorella at a ratio of 16:00, wherein the feeding density is 80-100 per mL; 19:00, feeding expanded feed with the particle size of 2 mm and the crude protein content of less than 30 percent, wherein the feeding amount is 10-13 percent of the weight of the fish; after three days of nutrition enrichment, the trachinotus ovatus can be transported out of the culture treatment system for normal culture.
6. The culture treatment system for trachinotus ovatus according to claim 1, wherein the culture treatment system comprises: the culture treatment system for the trachinotus ovatus is not only suitable for the trachinotus ovatus infected with cryptocaryon irritans or suspected to be infected with cryptocaryon irritans, but also suitable for the trachinotus ovatus which is not diseased.
7. The culture treatment system for trachinotus ovatus according to claim 1, wherein the culture treatment system comprises: the concentration of the acetic acid aqueous solution is 100ml/m3。
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