CN101940181B - Water saving-type farming method of turbot - Google Patents

Water saving-type farming method of turbot Download PDF

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CN101940181B
CN101940181B CN2010102709531A CN201010270953A CN101940181B CN 101940181 B CN101940181 B CN 101940181B CN 2010102709531 A CN2010102709531 A CN 2010102709531A CN 201010270953 A CN201010270953 A CN 201010270953A CN 101940181 B CN101940181 B CN 101940181B
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water
bdellovibrio
turbot
pond
farming
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CN101940181A (en
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蔡俊鹏
何伟杰
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South China University of Technology SCUT
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Abstract

The invention discloses a water saving-type farming method of turbot, comprising the selection of farming water source, the processing of feed, the control of water change period, and the control of farming environment by the bioecological method according to the ecology of farming water, the growing environment requirements of marine animals, the correlations of the animals and the standard of pollution-free farming. The key technology of the invention is characterized in that the bdellovibrio bacteriovorus nectophore liquid is added into the water, meanwhile the feed is soaked by the bdellovibrio bacteriovorus nectophore liquid. The water in the pool can be changed once in each 40 day during the farming period. The invention provides an animal farming method with the characteristics of green, safety, pollution-free and significant effect. The method can improve the water quality, promote the growth of the turbot, enhance the survival rate and immunity of the turbot, and have excellent development prospect. The invention has great significance to the enhancements of economic return and food safety, and the protection of human health.

Description

A kind of water-saving turbot cultural method
Technical field
The invention belongs to the marine fish culture technical field, particularly a kind of raising turbot immunity promotes the turbot growth, improves water quality, saves water and energy, uses manpower and material resources sparingly, the turbot cultural method of the novel non-pollution of green low-carbon.
Background technology
Turbot (Scophthalmus maximus) belongs to the Pleuronectiformes Bothidae, English name Turbot; Be commonly called as brill; Contain abundant colloid, no ossiculum scarifies in the body, visceral mass is little, dressing percentage is high, abundant delicate, the delicious flavour of muscle, nutritious, enjoy the consumer to like.Turbot was introduced over 10 years, and with its breeding advantage, advanced innovation mode and industrialized aquaculture trend become the coastal noticeable important industry of breeding fish of northern China.Yet, along with turbot aquaculture flourish in China, the particularly quick growth of industrial aquaculture scale, breeding environment constantly worsens, and disease is on the rise, and these have become the bottleneck of its aquaculture sustainable development of restriction.
" green house+phreatic water is cultured " is the most general production model of present aquaculture.This aquaculture model needs a large amount of water circulations, and the exhaustive exploitation of underground well water uses, and not only wastes the resource and the energy, causes water resource exhausted, also can have a strong impact on geologic aspects, for geological disaster hides some dangers for.A large amount of breeding wastewaters discharges, and also causes the severe contamination of ocean water body.
Manufactured feed and chemicals, a large amount of water circulations is carried out the fish intensive culture of the low output of high investment, has increased input cost greatly.Traditional antibiotic and chemicals quote many adverse consequencess such as causing endurance strain increase, the sexual organ infringement of fish medicine source, medicament residue, harm ecotope, cause the decline and the outlet obstacle of aquatic products quality.Therefore, " green cultivation " becomes the great demand of country in recent years, causes the extensive concern of aquatic products circle.At present, improve the immunity of turbot, promote the turbot breeding way report as yet at home and abroad of growth fast with the Bdellovibrio telotroch.
Existing research shows, as a kind of effective microorganism preparation, no matter is in food industry or in fields such as (ocean) aquacultures, the application of Bdellovibrio all is safe.For example: abroad; Lenz and Hespell (1978) discovers; Bdellovibrio and animal and people's cell do not had an infectivity [Lenz R.W.; Hespell R.B.Attempts to grow bedellovibrios micurgically-injected into animal cells.Archives of Microbiology, 1978,119 (3): 245-248].At home, Lin Mao etc. (2006) have studied the effect of Bdellovibrio to fish cell, find that it does not have dissemination [Lin Mao, Yang Xianle, Xue Hui, Cao Haipeng, Qiu Junqiang to the fish bacterium.The effect of 02 pair of fish cell of Bdellovibrio BDH21 and pathogen.The microbiology circular, 2006,33 (1): 7-11].
Summary of the invention
For overcoming the deficiency that exists in the above-mentioned existing cultural technique, the object of the present invention is to provide a kind of water-saving turbot cultural method; This method realizes through in feed and water body, adding the Bdellovibrio telotroch, specifically comprises the breed choosing source, the processing of Chi Shui, the processing of feed; The control of exchange water cycle, its characteristic is learned principle according to breeding ecological, halobiontic growing environment requirement, correlation between the biology and nuisanceless breed standard; The artificial breeding environment of creating reduces input cost, improves water quality; Improve turbot immunity, promote turbot to grow fast, increase economic efficiency.
The object of the invention is realized through following technical proposals: a kind of water-saving turbot cultural method comprises following operating procedure:
(1) gets the free of contamination seawater in marine site as breeding water; Breeding water injects through sand filtration, post precipitation cultures the pond; Above culturing the pond, cover gobo, the control intensity of illumination is 150～200lux;
(2) in culturing the pond, add Bdellovibrio telotroch bacterium liquid, and strengthen aeration, stir, make the concentration of the Bdellovibrio telotroch of Chi Shuizhong reach 10～10 7Pfu/mL;
(3) in culturing the pond, throw in fry;
(4) normal aeration during the breed, the control water temperature is at 15～30 ℃, and daily ration of feeding is the mass percent 3～5% of fish body weight, and bait uses concentration before input be 10～10 7The Bdellovibrio telotroch bacterium liquid of pfu/mL soaks 15～30min; Every changed Chi Shui (full pond) at a distance from 5～40 days once, change adding Bdellovibrio telotroch bacterium liquid behind the water at every turn, make the Bdellovibrio telotroch concentration of Chi Shuizhong reach 10～10 7Pfu/mL.
Described Bdellovibrio telotroch is that Bdellovibrio is free in the outside state of host bacterium;
At the bottom of the pond in the said breed of step (1) pond is matrix or flat, and cleaning and sterilizing is clean before injecting breeding water to culture the pond; The shading rate of said gobo is 95%.
The method of the said adding in step (2) and (4) Bdellovibrio telotroch bacterium liquid is: behind the Chi Shui dilution bacterium liquid of culturing in the pond, and the full pond of evenly splashing.
The aeration rate of the said increasing aeration of step (2) is 90～100L/h/m 3The aeration rate of the said normal aeration of step (4) is 70～80L/h/m 3
The said input of step (3) fry when 15～30 ℃ of water temperatures according to following standard: when fry weight was 25～50g, input density was 300～400 tails/m 2When fry weight was 70～100g, input density was 200～300 tails/m 2When fry weight was 100～200g, input density was 100～150 tails/m 2Temperature then reduces cultivation density when raising.
The said bait of step (4) is granular bait for fish; Said bait in the morning, noon and evening each throw in once, each bait throwing in 5～6 hours at interval, the bait throwing in mode is for perfusing full pond.
Step (2) and (4) said Bdellovibrio are Bdellovibrio (Bdellovibrio sp.) BDFM05, and said Bdellovibrio is preserved in Chinese typical culture collection center on August 7th, 2009, and deposit number is CCTCC NO:M 209172.
Said Bdellovibrio BDFM05 carries out under electron microscope, carrying out morphologic observation: BDFM05 after the negative staining and is unicellular, ellipse, and size is 1.43 * 0.53um, and end is given birth to flagellum, and flagellum length is 2um at least; Said Bdellovibrio BDFM05 cultivates the transparent circular plaque that can form diameter 2～3mm in three days with the double-layer plate method in 28 ℃;
Said Bdellovibrio BDFM05 is obtained through ultraviolet mutagenesis by wild type Bdellovibrio BDS02;
Said Bdellovibrio telotroch bacterium liquid prepares according to following method: inoculation Aeromonas hydrophila (Aeromonas hydrophila purchases the DSMZ of Institute of Micro-biology in Guangdong Province, numbering GIM1.172) is in the nutrient broth liquid nutrient medium; Cultivate 20h for 26～30 ℃, culture fluid is behind the centrifugal 10～20min of 5000～8000rpm, and deposition is added to DNB liquid nutrient medium (nutrient broth 0.8g; Caseinic acid hydrolysate 0.5g, yeast extract 0.1g is dissolved in the 1000mL distilled water; The pH value is 7.2～7.6) in; Insert Bdellovibrio BDFM05, cultivate 36～48h for 28 ℃, culture fluid is behind the centrifugal 15～20min of 6000～8000rpm; With the supernatant of Bdellovibrio telotroch behind the centrifugal 15～20min of 16000～18000rpm; Deposition use DNB liquid nutrient medium, water, physiological saline or 0.2mol/L pH value are 7.2～7.6 phosphate buffer suspension, obtain Bdellovibrio telotroch bacterium liquid, and the concentration of Bdellovibrio BDFM05 telotroch bacterium liquid can reach 10 9Pfu/mL.
Said DNB liquid nutrient medium is that nutrient broth 0.8g, caseinic acid hydrolysate 0.5g and yeast extract 0.1g are dissolved in the 1000mL distilled water, regulates pH value to 7.2～7.6.
Compared with prior art, the present invention has following beneficial effect:
Developing nuisanceless breed, improve the immunity of turbot, promote the quick growth of turbot, reduce production costs, increase economic efficiency, is the only way of China's mariculture industry development.Bdellovibrio can be improved the aquaculture organism intestinal environment, improves immunity, promotes growth.To the fish free of toxic effects, to human no pathogenicity.
It is very fast that the Bdellovibrio telotroch is invaded the process of host bacteria, can accomplish in general several seconds.For Bdellovibrio leech plastid, though exist the weakness that preparation is loaded down with trivial details, be difficult for to preserve, with the control of Bdellovibrio telotroch with to eliminate the ocean pathogenic or potentially pathogenic organism is rapid-action, energetic.These advantages make the Bdellovibrio telotroch be suitable as equally to restrain or remove and cause a disease in organism and the environment thereof or the biological cleaning factor of potentially pathogenic organism.
Because the Bdellovibrio telotroch has strong cracking strength to pathogenic or the potentially pathogenic organism, particularly vibrio marinopraesens of aquatic products, and water quality is also had certain catharsis; Soak feed with the Bdellovibrio telotroch, and the certain density Bdellovibrio telotroch bacterium liquid of splashing in the pool water within, the growth of turbot can be promoted; Improve immunity, can purify water again, and length can be changed (full pond) in 40 days, and water once; Thereby side effect and a large amount of breeding wastewater exhaust emission environment that the antibiotic abuse brings have been overcome; Use manpower and material resources sparingly,, improve raiser's income for the mankind provide safety, green, non-harmful aquatic products.
Embodiment
Below in conjunction with embodiment the present invention is done further detailed description, but embodiment of the present invention is not limited thereto.
Practical implementation of the present invention place is a Shandong Province plant, and the enforcement time is 8 months.Experimental field the source quality in marine site meets State Standard of the People's Republic of China's water quality standard for fishery (GB11607-89) requirement; Get into after the sand filtration of seawater process, the precipitation process and culture the pond, meet (NY5052-2001) requirement of Ministry of Agriculture's " pollution-free food mariculture water water quality ".The breed pond of turbot is built in and is cultured in the booth, and each sump area is 40m 2, 1.2 meters of the depth of waters, the matrix of trying one's best at the bottom of the pond helps operations such as draining soil pick-up; But also can have flat.It is 95% gobo that pond top covers obscurity, avoids illumination strong excessively.Intensity of illumination is controlled at 150～200lux.
Select physical health, individual turbot of uniform size (Scophthalmus maximus) juvenile fish, average weight is 28 ± 2.5g, and average body length is 11 ± 0.55cm, adopts the randomized block experiment design, according to 300 tails/m 2Cultivation density drop in the pond, be equally divided into A-D4 experimental group, B, C, each experimental group of D have 8 groups, are respectively B1, C1, D1; B2, C2, D2; , B3, C3, D3; B4, C4, D4; B5, C5, D5; B6, C6, D6; B7, C7, D7; B8, C8, B8; The every group of B1～D8 is further divided into 4 groups, i.e. B1-1～B1-4 ... D8-1～D8-4, every group of 2 repetitions.Test processing is following:
A group: normal flowing water, the normal pellet of throwing something and feeding (breeding way of plant produced);
B group: the Bdellovibrio telotroch bacterium liquid of in water, splashing makes that the telotroch bacterial concentration reaches 10,10 respectively in the water body 3, 10 5, 10 7Pfu/mL throws in pellet;
The C group: the concentration of throwing something and feeding is 10,10 3, 10 5, 10 7Pellet after the Bdellovibrio telotroch bacterium liquid of pfu/mL soaks;
D group: in water, add Bdellovibrio telotroch bacterium liquid, make cell concentration reach 10,10 respectively 3, 10 5, 10 7Pfu/mL, the concentration of throwing something and feeding respectively simultaneously is 10,10 3, 10 5, 10 7Pellet after the Bdellovibrio telotroch bacterium liquid of pfu/mL soaks;
B1, C1, D1 organize per 5 days and change (full pond) water once, add bacterium again after changing water;
B2, C2, D2 organize per 10 days and change (full pond) water once, add bacterium again after changing water;
B3, C3, D3 organize per 15 days and change (full pond) water once, add bacterium again after changing water;
B4, C4, D4 organize per 20 days and change (full pond) water once, add bacterium again after changing water;
B5, C5, D5 organize per 25 days and change (full pond) water once, add bacterium again after changing water;
B6, C6, D6 organize per 30 days and change (full pond) water once, add bacterium again after changing water;
B7, C7, D7 organize per 35 days and change (full pond) water once, add bacterium again after changing water;
B8, C8, D8 organize per 40 days and change (full pond) water once, add bacterium again after changing water;
B1-1, C1-1, D1-1 ... The bacterial concentration that D8-1 throws in is 10pfu/mL;
B1-2, C1-2, D1-2 ... The bacterial concentration that D8-2 throws in is 10 3Pfu/mL;
B1-3, C1-3, D1-3 ... The bacterial concentration that D8-3 throws in is 10 5Pfu/mL;
B1-4, C1-4, D1-4 ... The bacterial concentration that D8-4 throws in is 10 7Pfu/mL;
Particles used People's Republic of China's agricultural industry criteria " the manufactured feed safety limit is used in the pollution-free food fishing " regulation (NY5072-2002) that meets.Duration of test, the mass fraction of daily ration of feeding and fish body weight is 3-5%, throwing something and feeding every day, three times (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.Growth indexes, the immune indexes of fish measured in off-test.
(1), the assay method of fish bulk-growth index is following:
Randomly draw 40 tail fishes for every group and detect growing state, length of fish body is measured and adopted precision is the slide measure tolerance of 1mm, and body is long holds the distance of vertebrae between bottom for the kiss of fish.Body weight adopts electronic balance weighing.The test growth indexes is calculated by following formula:
Survival rate (%)=N t/ N o* 100%;
Rate of body weight gain (%)=(W t-W o)/W o* 100%;
The long growth rate (%) of body=(L t-L o)/L o* 100%;
Coefficient of condition=W o/ L iOr W t/ L e
N in the formula oBe the total mantissa of on-test macrura reevesii body; N tBe the total mantissa of off-test macrura reevesii body; W oBe on-test macrura reevesii body weight; W tBe off-test macrura reevesii body weight; L oFish length during for on-test; L tFish length during for off-test;
Turbot each item growth indexes that test records is seen table one～table four: can find out that from table A composition motility rate, rate of body weight gain, the long growth rate of body, coefficient of condition are respectively 81.45%, 1792%, 56.65%, 21.43.Compare with the A group, B, C, the survival rate of D group, rate of body weight gain, the long growth rate of body, coefficient of condition all are significantly improved.Explanation is under the breeding way of routine, and not changing water can influence the survival rate of turbot, rate of body weight gain, the long growth rate of body, coefficient of condition.Under identical exchange water cycle, along with the rising of Bdellovibrio concentration, survival rate, rate of body weight gain, the long growth rate of body, coefficient of condition are all raising gradually, and are all organizing apparently higher than A.
B1-1～B8-1 ... Survival rate between D1-4～D8-4, rate of body weight gain, the long growth rate of body, coefficient of condition no significant difference.Explanation is in that water neutralization/or in feed, to add concentration be 10～10 7Pfu/mL Bdellovibrio telotroch bacterium liquid changed (full pond) water in 5-40 days once, and is not obvious to survival rate, rate of body weight gain, the long growth rate of body, coefficient of condition influence, all can promote growth.
This shows that no matter in water body, still adding concentration in the feed is 10～10 7Pfu/mL Bdellovibrio telotroch bacterium liquid changed (full pond) water once, and all can promote the turbot growth in 40 days.Under the identical exchange water cycle, Bdellovibrio telotroch bacterial concentration is high more, and effect is good more.In water with in the feed, add simultaneously, effect is better.
The detection of table one turbot growth indexes
Figure BSA00000254527200071
The detection of table two turbot growth indexes
Figure BSA00000254527200072
Figure BSA00000254527200081
The detection of table three turbot growth indexes
Figure BSA00000254527200082
Figure BSA00000254527200091
The detection of table four turbot growth indexes
Figure BSA00000254527200101
(2), the assay method of fish body immune indexes is following:
1, antalzyme activity is measured
Serum lysozyme active through the turbidity colorimetric method for determining (Ellis A E.Lysozymeassays. [M] //Stolen J S; Fletcher T C; Anderson D P, et al.Techniques in FishImmunology I.USA:SOS Publications, 1990:101-103.)
Under the experiment condition, it is 1 unit of enzyme activity (U) that the every min light absorption value of every mL serum reduces 0.001.
2, the mensuration of phenol oxidase (PO) vigor
Phenoloxidase activity is measured (Hern á ndez-L ó pez J such as adopting Hern á ndez-L ó pez; Gollas-Galvan T.Vargas-Albores F.Activation of the prophenoloxidase system ofthe brown Penaeus californiensis Holmes [J] .Comp Biochem Physiol, method 1996.113C:61-66.).Under experiment condition, the every min extinction of every mL serum increases by 0.001, is defined as 1 unit of enzyme activity (U).
3, superoxide dismutase (SOD) vigor
Superoxide dismutase (SOD) vigor advances by improved 1,2,3,-thrihydroxy-benzene autoxidation methods such as Deng Biyu.(Deng Biyu, Yuan Qinsheng, Li Wenjie. the 1,2,3,-thrihydroxy-benzene autoxidation of improvement is measured the method [J] of superoxide dismutase activity. biochemistry and biophysics progress, 1991,18 (2): 163～163)
The mensuration result of turbot each item immune indexes sees table five～table eight:
Can find out that from table A group antalzyme activity, superoxide dismutase activity, phenol oxidase vigor are respectively 18.06U/mL, 209.78U/mL, 5.58U/mL.Compare with the A group, antalzyme activity, superoxide dismutase activity, the phenol oxidase vigor of B, C, D group all are significantly improved.Compare with the A group, antalzyme activity, superoxide dismutase activity, the phenol oxidase vigor of B, C, D group all are significantly improved.Explanation is under the breeding way of routine, and not changing water can influence the antalzyme activity of turbot, superoxide dismutase activity, phenol oxidase vigor.Under identical exchange water cycle, along with the concentration rising of Bdellovibrio telotroch, antalzyme activity, superoxide dismutase activity, phenol oxidase vigor are all raising gradually.The concentration that the Bdellovibrio telotroch is described is high more, and the immunity of turbot is strong more.B1-1～B8-1 ... Antalzyme activity between D1-4～D8-4, superoxide dismutase activity, phenol oxidase vigor no significant difference.Be illustrated in that water neutralization/or in feed, to add concentration be 10～10 7Pfu/mL Bdellovibrio telotroch bacterium liquid changed (full pond) water in 5-40 days once, and is little to antalzyme activity, superoxide dismutase activity, phenol oxidase effect of vigor, all can improve immunity.
Result of the test shows that the breeding way of B, C, D group all can improve the immunity of fish, promote growth, and D group best results.That is: no matter in water or in feed, adding concentration is 10～10 7Pfu/mL Bdellovibrio telotroch bacterium liquid changed (full pond) water and once all can improve antalzyme activity, superoxide dismutase activity, the phenol oxidase vigor of turbot in 40 days; The concentration of Bdellovibrio telotroch is high more, and effect is good more.In water with in the feed, add best results simultaneously.
A group total number of bacteria and vibrios sum are respectively 2.75 * 10 3Cfu/mL and 6.48 * 10 2Cfu/mL.The total number of bacteria of B, C, D group is respectively 5.98 * 10 2Cfu/mL, 6.99 * 10 2Cfu/mL, 4.63 * 10 2Cfu/mL; Vibrios sum 8.5 * 10cfu/mL, 9.1 * 10cfu/mL, 4.6 * 10cfu/mL compare with the A group, have all reduced by 1 one magnitude.The pH of A, B, C, D group is all between 7.78～8.12.
This shows no matter in water or in feed, adding concentration is 10～10 7The Bdellovibrio telotroch bacterium liquid of pfu/mL all can improve immunity, the promotion of fish and grow, but also the potential pathogenic or potentially pathogenic organism in the controlled water body etc., and then improve water quality.In water, use simultaneously with feed, effect is optimum.
The mensuration result of table five immune indexes
Figure BSA00000254527200121
Figure BSA00000254527200131
The mensuration result of table six immune indexes
Figure BSA00000254527200141
The mensuration result of table seven immune indexes
Figure BSA00000254527200142
The mensuration result of table eight immune indexes
Figure BSA00000254527200151
D group in the foregoing description is preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.

Claims (7)

1. water-saving turbot cultural method is characterized in that comprising following operating procedure:
(1) gets the free of contamination seawater in marine site as breeding water; Breeding water injects and cultures the pond through sand filtration, post precipitation; Above culturing the pond, cover gobo, the control intensity of illumination is 150～200lux;
(2) in culturing the pond, add Bdellovibrio telotroch bacterium liquid, and strengthen aeration, stir, make the initial concentration of the Bdellovibrio telotroch of Chi Shuizhong reach 10～10 7Pfu/mL; Said Bdellovibrio is Bdellovibrio (Bdellovibrio sp.) BDFM05, and said Bdellovibrio is preserved in Chinese typical culture collection center on August 7th, 2009, and deposit number is CCTCC NO:M 209172;
(3) in culturing the pond, throw in fry;
(4) normal aeration during the breed, the control water temperature is at 15～30 ℃, and daily ration of feeding is the mass percent 3～5% of fish body weight, and bait is before input, and using concentration is 10～10 7Pfu/mL Bdellovibrio telotroch bacterium liquid soaks 15～30min; Whenever changed Chi Shui once, change at every turn and add Bdellovibrio telotroch bacterium liquid behind the water, make the Bdellovibrio telotroch concentration of Chi Shuizhong reach 10～10 at a distance from 5～40 days 7Pfu/mL; Said Bdellovibrio is Bdellovibrio (Bdellovibrio sp.) BDFM05, and said Bdellovibrio is preserved in Chinese typical culture collection center on August 7th, 2009, and deposit number is CCTCC NO:M 209172;
The said input of step (3) fry when 15～30 ℃ of water temperatures according to following standard: when fry weight was 25～50g, input density was 300～400 tails/m 2When fry weight was 70～100g, input density was 200～300 tails/m 2When fry weight was 100～200g, input density was 100～150 tails/m 2
2. a kind of water-saving turbot cultural method according to claim 1 is characterized in that: at the bottom of the pond in the said breed of step (1) pond is matrix or flat, and cleaning and sterilizing is clean before injecting breeding water to culture the pond; The shading rate of said gobo is 95%.
3. a kind of water-saving turbot cultural method according to claim 1 is characterized in that: the method for the said adding in step (2) and (4) Bdellovibrio telotroch bacterium liquid is: behind the Chi Shui dilution bacterium liquid of culturing in the pond, and the full pond of evenly splashing.
4. a kind of water-saving turbot cultural method according to claim 1 is characterized in that: the aeration rate of the said increasing aeration of step (2) is every cubic metre of water of 90～100L/h; The aeration rate of the said normal aeration of step (4) is every cubic metre of water of 70～80L/h.
5. a kind of water-saving turbot cultural method according to claim 1 is characterized in that: the said bait of step (4) is granular bait for fish; Said bait in the morning, noon and evening each throw in once, each bait throwing in 5～6 hours at interval, the bait throwing in mode is for perfusing full pond.
6. a kind of water-saving turbot cultural method according to claim 1; It is characterized in that: said Bdellovibrio telotroch bacterium liquid prepares according to following method: inoculation Aeromonas hydrophila (Aeromonas hydrophila) is in the nutrient broth liquid nutrient medium; Cultivate 20h for 26～30 ℃, culture fluid is behind the centrifugal 10～20min of 5000～8000rpm, and deposition is added in the DNB liquid nutrient medium; Insert Bdellovibrio BDFM05; Cultivate 36～48h for 28 ℃, culture fluid behind the centrifugal 15～20min of 6000～8000rpm, with the supernatant of Bdellovibrio telotroch behind the centrifugal 15～20min of 16000～18000rpm; Deposition use DNB liquid nutrient medium, water, physiological saline or 0.2mol/L pH value are 7.2～7.6 phosphate buffer suspension, and obtaining concentration is 10 9Pfu/mL Bdellovibrio telotroch bacterium liquid.
7. a kind of water-saving turbot cultural method according to claim 6; It is characterized in that: said DNB liquid nutrient medium is that nutrient broth 0.8g, caseinic acid hydrolysate 0.5g and yeast extract 0.1g are dissolved in the 1000mL distilled water, regulates pH value to 7.2～7.6.
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