CN114651712A - Storage method for preserving vitality of quercus mongolica pollen - Google Patents
Storage method for preserving vitality of quercus mongolica pollen Download PDFInfo
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- CN114651712A CN114651712A CN202210302192.6A CN202210302192A CN114651712A CN 114651712 A CN114651712 A CN 114651712A CN 202210302192 A CN202210302192 A CN 202210302192A CN 114651712 A CN114651712 A CN 114651712A
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- 238000003860 storage Methods 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 26
- 241001480055 Quercus mongolica Species 0.000 title claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 46
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims abstract description 25
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 8
- 239000008223 sterile water Substances 0.000 claims abstract description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 72
- 229920001131 Pulp (paper) Polymers 0.000 claims description 36
- 238000004321 preservation Methods 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 8
- 230000010152 pollination Effects 0.000 abstract description 5
- 238000009396 hybridization Methods 0.000 abstract 1
- 230000010154 cross-pollination Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 241000219492 Quercus Species 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 239000012535 impurity Substances 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000219428 Fagaceae Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000010196 hermaphroditism Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 235000001705 insufficient nutrition Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01D—HARVESTING; MOWING
- A01D91/00—Methods for harvesting agricultural products
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Abstract
The invention discloses a storage method for preserving the vitality of quercus mongolica pollen, which comprises the following steps: firstly, cutting off the flowering branches before powder scattering, putting the flowering branches into a sterile water culture container subjected to high-temperature sterilization and disinfection, and performing water culture indoors; step two, scattering pollen of the flowering branches which begin to scatter pollen and collecting scattered pollen; filtering the collected pollen, separating the pollen from the branches, leaves and petals doped in the pollen, and drying the pollen; and step four, putting the dried pollen into a collection container, sealing the collection container, and then putting the collection container into a refrigerator for storage. The invention relates to the technical field of pollen collection, and the method can realize long-time storage of quercus mongolica pollen and keep the activity of the quercus mongolica pollen after picking out flowering branches 1-7 days before pollen scattering, ensures flowering pollination in the second year, and meets the requirements of hybridization pollination.
Description
Technical Field
The invention relates to the technical field of pollen collection, in particular to a storage method for preserving the vitality of quercus mongolica pollen.
Background
Quercus mongolica Fisch ex Ledeb belonging to Fagaceae, Quercus, deciduous arbor, up to 30 m, and dark longitudinal split. The crown is oval. Alternate single leaf, inverted oval shape of leaf, wavy tooth edge of leaf, dark green surface of leaf, light green back, parthenocarpy, hermaphrodite, oval or elliptical shape of nut, and fleshy cotyledon of seed. Cellular chromosomes: 2 n-24. Is a second-grade precious tree species in China and is also a main secondary tree species in the northeast forest region of China. Mainly distributed in northeast, northwest and northwest China, and a small amount in China.
As the male and female flowers are asynchronous or the temperature in the quercus mongolica distribution area is different, the pollen in the current year sometimes cannot meet the requirement of cross pollination, and the pollen needs to be stored and kept alive as much as possible so as to ensure the pollination for flowering in the next year.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention aims to provide a storage method for preserving the vitality of quercus mongolica pollen, which can preserve the vitality of quercus mongolica pollen for a long time and ensure the need of cross pollination of quercus mongolica in the second year.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme, and the storage method for preserving the vitality of quercus mongolica pollen adopts the technical scheme that the storage method comprises the following steps:
firstly, cutting off the flowering branches before powder scattering, putting the flowering branches into a sterile water culture container subjected to high-temperature sterilization and disinfection, and performing water culture indoors;
step two, scattering pollen of the flowering branches which begin to scatter pollen and collecting scattered pollen;
filtering the collected pollen, separating the pollen from branches, leaves and petals doped in the pollen, and drying the pollen;
and step four, putting the dried pollen into a collection container, sealing the collection container, and then putting the collection container into a refrigerator for storage.
Preferably, the hydroponic culture comprises the following steps:
step one, preparing an aseptic water culture container after high-temperature sterilization and disinfection, and adding a sufficient amount of clear water into the water culture container;
cutting off the cut end of the branch with the flower, which is far away from one end of the flower, 5-8 cm away from the leaf at the bottommost end by using a knife, removing the leaf close to the flower, and inserting the end, which is far away from the cut end of the flower, of the branch with the flower into clear water of a sterile water culture container;
and step three, placing the flower branches inserted into the water culture container in a bright place far away from the sunlight indoors, and culturing at the room temperature of 20-25 ℃.
Preferably, the picking standard of the flowering branches is as follows: picking the branch with flower 1-7 days before the scattered powder.
Preferably, the pollen collection comprises the following steps:
taking out the branch with the flower, which is hydroponically cultured to loose powder, from a culture container, sleeving a sulfuric acid paper bag outside the branch with the flower to ensure that the flower is completely positioned in the sulfuric acid paper bag, and sealing and fixing the opening of the sulfuric acid paper bag on the branch with the flower by using a paper clip;
step two, forcibly shaking the sulfuric acid paper bag and the flowering branches, so that pollen on the flowers of the flowering branches is scattered in the sulfuric acid paper bag;
step three, opening the sulfuric acid paper bag, taking out the branch with flowers, and pouring the pollen scattered in the inner cavity of the sulfuric acid paper bag into a freeze dryer for drying;
and step four, pouring the pollen dried by the freeze dryer into a filter sieve for filtering.
Preferably, the temperature condition for collecting the pollen is 20-25 ℃.
Preferably, the drying temperature of the freeze dryer is 13-20 ℃.
Preferably, the pollen filtration comprises the following steps: pouring the pollen dried by the freeze dryer into a filter sieve, shaking the filter sieve to enable the pollen to fall into a collection container, and pouring the filtered petals and branches into a garbage can for treatment to finish the filtration treatment of the pollen.
Preferably, the preservation of pollen comprises the steps of: and sealing the collection container filled with the pollen, and storing the collection container in a refrigerator at the temperature of-20-80 ℃.
(III) advantageous effects
Compared with the prior art, the invention provides a storage method for preserving the vitality of quercus mongolica pollen, which has the following beneficial effects:
1. the method has the advantages that the water culture, collection, filtration and preservation are carried out after the flowering branches 1-7 days before pollen scattering are picked, so that the long-time storage of the quercus mongolicus pollen and the vitality maintenance of the quercus mongolicus pollen can be realized, the flowering pollination in the second year is guaranteed, and the requirement of cross pollination is met; cutting off the part, which is 5-8 cm away from the bottommost leaf, of one end of the flower-carrying branch far away from the flower by using a knife, removing the leaves close to the flower, improving the stability of the flower-carrying branch in water culture, avoiding the death of the flower-carrying branch in the water culture process, and culturing the flower-carrying branch, which is inserted into a water culture container together with the water culture container, at a bright place far away from the sunlight emission indoors at the room temperature of 20-5 ℃ to meet the normal survival of the flower-carrying branch;
2. the water culture effect can be improved by picking flowering branches 1-7 days before the loose powder is picked; the sulfuric acid paper bag is sleeved outside the branch with the flower, so that the flower is completely positioned inside the sulfuric acid paper bag, and the opening of the sulfuric acid paper bag is sealed and fixed on the branch with the flower by using a paper clip, so that scattered pollen can be completely scattered inside the sulfuric acid paper bag, the pollen can be conveniently collected, and pollen waste caused by scattering of the pollen everywhere can be avoided; pollen scattered in the inner cavity of the sulfuric acid paper bag is poured into a freeze dryer for drying, so that the pollen can be conveniently stored, and the storage time of the pollen is prolonged; the pollen-containing collecting container is sealed and placed in a refrigerator to be stored at the temperature of minus 20-80 ℃, the pollen can be stored for a long time by placing the pollen-containing collecting container in the refrigerator at the temperature of minus 20-80 ℃, the activity of the pollen can be kept in the storage process, the pollen can be guaranteed to bloom and pollinate in the next year, and the requirement of cross pollination is met.
Drawings
FIG. 1 is a graph showing the relationship between the preservation time and the in vitro germination rate of the present invention.
Detailed Description
The invention will be further elucidated and described with reference to the embodiments and drawings of the specification:
referring to fig. 1, the present invention: the storage method comprises the following steps: cutting the flowering branches before pollen scattering, putting the flowering branches into an aseptic water culture container after high-temperature sterilization and disinfection, carrying out water culture indoors, conveniently and timely collecting pollen of the flowering branches which begin to scatter the pollen by carrying out water culture on the flowering branches, avoiding pollen scattering caused by failure in timely collection when the quercus mongolica growing in the field scatters the pollen, secondly, scattering the pollen of the flowering branches which begin to scatter the pollen and collecting the scattered pollen, thirdly, filtering the collected pollen, separating the pollen from branches and leaves and petals doped in the pollen, drying the pollen, avoiding impurity doping in the stored pollen and influencing the quality of the pollen, fourthly, putting the dried pollen into a collection container, putting the collection container into a refrigerator after sealing, storing the pollen in the collection container at low temperature by using the refrigerator, can realize the long-time storage of pollen and keep the vitality of the quercus mongolica pollen, ensure the flowering and pollination in the second year and meet the requirement of cross pollination.
In this embodiment: hydroponic cultivation comprises the following steps: step one, preparing an aseptic water culture container after high-temperature sterilization and disinfection, avoiding the death of the flower-carrying branches of water culture caused by bacteria in the container, placing the water culture container in boiling water for boiling for a period of time when the water culture container is sterilized, meeting the purpose of sterilization and disinfection at the temperature of the boiling water, realizing the sterilization of the water culture container, adding a sufficient amount of clear water into the water culture container, providing a certain nutrition for the water culture of the flower-carrying branches through the clear water, adding a certain amount of nutrient solution into the clear water, avoiding the transnational situation that the flower-carrying branches die due to insufficient nutrition in the water culture process, improving the stability of the water culture, step two, cutting off the cut flower-carrying branches by a knife at a position 5-8 cm away from the bottommost leaves at one end of the flowers, removing the leaves close to the flowers, and removing the leaves close to the flowers, the method can avoid competition of nutrition between leaves and flowers and influence on development of the flowers, one end, far away from cut-off of the flowers, of the flower-bearing branch is inserted into clear water of a sterile water culture container, stability of the flower-bearing branch during water culture can be improved, and the situation that the flower-bearing branch dies during the water culture is avoided.
In this embodiment: picking standards of the boughs with flowers: picking the flowering branches 1-7 days before the pollen scattering, the water culture effect can be improved, if the picking time is too short from the pollen scattering time, pollen is inconvenient to collect in time, and if the picking time is too short from the pollen scattering time, the flowering branches need to be cultured for a long time, so that the pollen storage cost is increased.
In this embodiment: the pollen collection comprises the following steps: step one, taking out the boughs with flowers from a culture container, sleeving a sulfuric acid paper bag outside the boughs with flowers, enabling the flowers to be completely positioned inside the sulfuric acid paper bag, and sealing and fixing the tape openings of the sulfuric acid paper bag on the boughs with flowers by using clips, wherein the sulfuric acid paper bag has the characteristics of pure paper, high strength, good transparency, no deformation, sun resistance, high temperature resistance, ageing resistance and the like, can prevent pollen from scattering around due to the fact that the sulfuric acid paper bag is punctured by the boughs when the boughs with flowers are shaken, and waste of the pollen is caused, and by sleeving the sulfuric acid paper bag outside the boughs, the flowers are completely positioned inside the sulfuric acid paper bag, and the tape openings of the sulfuric acid paper bag are sealed and fixed on the boughs with flowers by using clips, the scattered pollen can be completely scattered inside the sulfuric acid paper bag, pollen is convenient to collect the pollen, and the pollen is prevented from scattering around, the pollen is prevented from flowing out of the bag opening of the sulfuric acid paper bag, the situation that the sulfuric acid paper bag is separated from the flower-carrying branch can be conveniently caused when the flower-carrying branch is shaken, the sulfuric acid paper bag and the flower-carrying branch are shaken forcibly to enable pollen on the flower of the flower-carrying branch to scatter inside the sulfuric acid paper bag, when the flower-carrying branch and the sulfuric acid paper bag are shaken, the flower-carrying branch and the inner wall of the sulfuric acid paper bag are collided to generate vibration, so that the pollen falls off and floats in the inner cavity of the sulfuric acid paper bag, the pollen is collected, the flower-carrying branch is taken out, the pollen scattered in the inner cavity of the sulfuric acid paper bag is poured into a freeze-drying machine to be dried, the pollen can be dried, the pollen is conveniently stored, and the pollen storage time is prolonged, and step four, pouring the pollen dried by the freeze dryer into a filter sieve for filtering.
In this embodiment: the temperature condition for pollen collection is 20-25 ℃, the activity of pollen in the collection process can be ensured, and the influence of pollen activity reduction caused by improper outside temperature on later-stage use of pollen is avoided.
In this embodiment: the drying temperature of the freeze dryer is 13-20 ℃, so that the pollen can be dried, and the storage time is prolonged.
In this embodiment: the pollen filtration comprises the following steps: pouring the pollen dried by the freeze dryer into a filter sieve, shaking the filter sieve to enable the pollen to fall into a collection container, pouring the filtered petals and branches and leaves into a garbage can for treatment, completing the filtration treatment of the pollen, and avoiding the influence on the quality of the pollen caused by impurities doped in the collected and stored pollen.
In this embodiment: the preservation of pollen comprises the following steps: the collection container filled with the pollen is sealed and is placed in a refrigerator to be stored at the temperature of minus 20-80 ℃, the pollen can be stored for a long time by placing the sealed collection container filled with the pollen in the refrigerator at the temperature of minus 20-80 ℃, the activity of the pollen can be kept in the storage process, the pollen can be guaranteed to bloom and pollinate in the next year, and the requirement of cross pollination is met.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (8)
1. A storage method for preserving the vitality of quercus mongolica pollen is characterized in that: the storage method comprises the following steps:
firstly, cutting off the flowering branches before powder scattering, putting the flowering branches into a sterile water culture container subjected to high-temperature sterilization and disinfection, and performing water culture indoors;
step two, scattering pollen for the branch with the flower which starts to scatter the pollen and collecting the scattered pollen;
filtering the collected pollen, separating the pollen from branches, leaves and petals doped in the pollen, and drying the pollen;
and step four, putting the dried pollen into a collection container, sealing the collection container, and then putting the collection container into a refrigerator for storage.
2. The storage method for preserving the vigor of quercus mongolica pollen according to claim 1, wherein: the hydroponic culture comprises the following steps:
step one, preparing an aseptic water culture container after high-temperature sterilization and disinfection, and adding a sufficient amount of clear water into the water culture container;
cutting off the cut end of the branch with the flower, which is far away from one end of the flower, 5-8 cm away from the leaf at the bottommost end by using a knife, removing the leaf close to the flower, and inserting the end, which is far away from the cut end of the flower, of the branch with the flower into clear water of a sterile water culture container;
and step three, placing the flower branches inserted into the water culture container in a bright place far away from the sunlight indoors, and culturing at the room temperature of 20-25 ℃.
3. The storage method for preserving the vigor of quercus mongolica pollen according to claim 1, wherein: the picking standard of the branch with flowers is as follows: picking the branch with flower 1-7 days before the scattered powder.
4. The storage method for preserving the vigor of quercus mongolica pollen according to claim 1, wherein: the pollen collection comprises the following steps:
taking out the branch with the flower, which is hydroponically cultured to loose powder, from a culture container, sleeving a sulfuric acid paper bag outside the branch with the flower to ensure that the flower is completely positioned in the sulfuric acid paper bag, and sealing and fixing the opening of the sulfuric acid paper bag on the branch with the flower by using a paper clip;
step two, forcibly shaking the sulfuric acid paper bag and the boughs to enable pollen on the flowers of the boughs to fall into the sulfuric acid paper bag;
step three, opening the sulfuric acid paper bag, taking out the branch with flowers, and pouring the pollen scattered in the inner cavity of the sulfuric acid paper bag into a freeze dryer for drying;
and step four, pouring the pollen dried by the freeze dryer into a filter sieve for filtering.
5. The storage method for preserving the vitality of quercus mongolica pollen according to claim 4, wherein: the temperature condition for collecting the pollen is 20-25 ℃.
6. The storage method for preserving the vitality of quercus mongolica pollen according to claim 4, wherein: the drying temperature of the freeze dryer is 13-20 ℃.
7. The storage method for preserving the vigor of quercus mongolica pollen according to claim 1, wherein: the pollen filtration comprises the following steps: pouring the pollen dried by the freeze dryer into a filter sieve, shaking the filter sieve to enable the pollen to fall into a collection container, and pouring the filtered petals and branches into a garbage can for treatment to finish the filtration treatment of the pollen.
8. The storage method for preserving the vigor of quercus mongolica pollen according to claim 1, wherein: the preservation of the pollen comprises the following steps: and sealing the collection container filled with the pollen, and storing the pollen in a refrigerator at the temperature of-20-80 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210302192.6A CN114651712A (en) | 2022-03-24 | 2022-03-24 | Storage method for preserving vitality of quercus mongolica pollen |
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| CN202210302192.6A CN114651712A (en) | 2022-03-24 | 2022-03-24 | Storage method for preserving vitality of quercus mongolica pollen |
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Application publication date: 20220624 |