CN114602326A - Lifting type filter membrane replacing device for environmental DNA sampling - Google Patents
Lifting type filter membrane replacing device for environmental DNA sampling Download PDFInfo
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- CN114602326A CN114602326A CN202210156593.5A CN202210156593A CN114602326A CN 114602326 A CN114602326 A CN 114602326A CN 202210156593 A CN202210156593 A CN 202210156593A CN 114602326 A CN114602326 A CN 114602326A
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- top cover
- filter
- base
- cake
- sliding block
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/08—Flat membrane modules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
Abstract
The invention provides a lifting type filter membrane replacing device for environmental DNA sampling, which comprises: the base is of a flat plate structure with a horizontal upper surface; the magazine type mechanism is internally provided with a plurality of filter cakes to be sampled; the sampling assembly is used for collecting a water sample of a filter cake to be sampled; the sampling assembly comprises a top cover, and a space for clamping a filter cake to sample the filter cake is formed between the top cover and the base; the conveying assembly is used for conveying the filter cake to the space and comprises a sliding block and a winding roll, the sliding block is arranged on the base and positioned at the port on the other side of the channel, the winding roll is arranged above the top cover, a first drawn wire is wound on the winding roll, the free end of the first drawn wire is vertically connected to the top cover, and the bottom of the top cover is connected with the sliding block through a second drawn wire; when the winding roll rotates, the top cover is pulled through the first wire drawing, the top cover pulls the sliding block through the second wire drawing, and the sliding block pushes a filter cake in the magazine type mechanism to a position between the top cover and the base for water sample collection.
Description
Technical Field
The invention belongs to the technical field of water sample collection, and particularly relates to a lifting type filter membrane replacing device for environmental DNA sampling.
Background
Along with the promotion of new strategies for global biodiversity management and the implementation of the 'biodiversity convention' of the united nations, the importance of biodiversity investigation and research is more and more prominent. With the development of biodiversity research, large-scale and high-frequency metagenomic analysis of environmental DNA and microorganisms in water is generally required, which requires large-scale filtration of water samples and high-frequency replacement of filtration membranes. Different from the common filter membrane replacement scene, in the environment DNA sampling or microorganism sampling scene, extra attention needs to be paid to avoiding cross contamination among samples and avoiding introducing new pollution in the operation process, so that the independence of the samples and the accuracy of the detection result are ensured. In the conventional method for simply and directly replacing the filter membrane, after a water sample is filtered by the disc filter membrane, an experimenter manually opens the filter head and takes out the filter membrane containing the sample by using clean tweezers, then the clean tweezers are used for clamping a new disc filter membrane to replace the filter head, and finally the filter head is manually installed again. Whether applied to long-term continuous monitoring at a fixed site or to sequential sampling of multiple sites following an investigation vessel, means a large scale, high frequency of replacement disc filter operations. The main problems and disadvantages of the prior art include: due to the current manual replacement of the disk filter membrane, the operation is complicated, and different experimenters may introduce errors due to different operation methods. Moreover, the operation flow of disassembling, taking the membrane, replacing the membrane and re-installing consumes a lot of time and labor, and is not favorable for sampling at high frequency. And thirdly, pollution is easily caused by manual replacement of the filter membrane, so that the deviation of the sample investigation result is caused. Finally, the manual replacement of the filter membrane is not easy to operate, is not beneficial to automatic sampling and filtering, and is not beneficial to standardized long-term continuous sampling or online continuous monitoring activities. The solutions for replacing the filter membranes by pneumatic adsorption used in other fields are liable to cause cross-contamination between the filter membranes. The scheme of replacing the filter membrane by the rotary disc type used in other fields has less filter membrane quantity which can be accommodated, and cannot meet the application of replacing the disc filter membrane scene at large scale and high frequency.
That is, in the conventional filter membrane replacement method, the filter membrane replacement is performed manually, which has the disadvantages of complicated operation, error introduced by different experimenters due to different operation methods, and low replacement efficiency.
Therefore, how to improve the replacement speed of the filter membrane replacement, and reduce the error in the replacement process, thereby improving the replacement efficiency of the filter membrane is a technical problem that needs to be solved urgently by technical personnel in the field.
Disclosure of Invention
The invention provides a pull-up filter membrane replacing device for environmental DNA sampling, which at least solves the technical problem;
in order to solve the above problems, a first aspect of the present invention provides a pull-up type filter membrane exchange device usable for environmental DNA sampling, the filter membrane being assembled with a filter cake of an integrated structure by a housing of an annular shape, the filter membrane exchange device comprising: the base is of a flat plate structure with a horizontal upper surface; the magazine type mechanism is arranged on the base, a plurality of filter cakes to be sampled are arranged in the magazine type mechanism, and a cake passing channel through which one filter cake can pass is arranged at the bottom of the magazine type mechanism; the sampling assembly is arranged on the base and positioned at one side port of the cake passing channel and is used for collecting a water sample of the filter cake; the sampling assembly comprises a top cover, and a cake containing space for clamping the filter cake is formed between the top cover and the base; the conveying assembly comprises a sliding block and a wire winding roll, the sliding block is arranged on the base and positioned at the port on the other side of the cake passing channel, the wire winding roll is arranged above the top cover, a first wire drawing is wound on the wire winding roll, the free end of the first wire drawing is vertically connected to the top cover, and the bottom of the top cover is connected with the sliding block through a second wire drawing; when the winding roll rotates, the top cover is pulled through the first wire drawing, the top cover pulls the sliding block through the second wire drawing, the sliding block penetrates through the port on the other side of the cake passing channel to push the filter cake to be sampled, and the filter cake penetrates through the port on one side of the cake passing channel to move to the cake containing space.
In the first aspect, a pair of magazine through holes are correspondingly formed in a pair of opposite side walls at the lower part of the cylindrical magazine type mechanism, and a plurality of filter cakes to be sampled are arranged in the magazine type mechanism in an up-and-down overlapping mode; the filter membrane replacing device further comprises: the limiting column is vertically arranged on the top cover, and the upper part of the limiting column is in a conical shape; the pair of crank levers are of long rod structures, interference parts are arranged at two ends of each long rod structure, the middle of each long rod structure is connected with the outer wall of the magazine type mechanism through a rotating shaft, one interference part of each long rod structure is arranged in the corresponding magazine through hole and is in contact with the filter cake, and the other interference part of each long rod structure is in contact with the upper part of the limiting column; when the top cover is in an initial position of initial rising, the pair of interference parts of the long rod structure are correspondingly in natural contact with the filter cake and the limiting column, when the top cover is displaced upwards, the conical surface of the limiting column pushes the interference part at the other end of the long rod structure, and the long rod structure rotates around the rotating shaft, so that the interference part at one end of the long rod structure is in close contact with the filter cake at the corresponding side.
In the first aspect, a pulley block is further arranged on the first drawn wire.
In a first aspect, the filter membrane replacing device further includes an elastic member having a telescopic function, one end of the elastic member is connected to one end of the slider away from the magazine mechanism, the other end of the elastic member is connected to the base, and when the elastic member is in a natural extension state, the slider is located at an initial moving position.
In the first aspect, a sliding groove is formed in the base, the direction of the sliding groove is opposite to the other side port, and the sliding block is arranged in the sliding groove and can slide along the sliding groove.
In the first aspect, a guide channel penetrating through the front and back sides of the base is arranged on the base, the guide channel is arranged in the chute and has the same direction with the chute, a sliding column is arranged at the bottom of the sliding block, and the sliding column penetrates through the guide channel and is positioned at the bottom of the base; one end of the second drawn wire is connected with the sliding column, and the other end of the second drawn wire is connected to the top cover through the base in a penetrating manner; the elastic component sets up the lower surface of base, just the one end of elastic component with sliding column connects, the other end of elastic component with the base is kept away from the end connection of magazine-type mechanism.
In the first aspect, a through limiting hole is further formed in the cylinder of the sliding column, the second drawn wire penetrates through the limiting hole to be connected with the elastic piece, the arrangement directions of the second drawn wire and the spring are consistent with the arrangement direction of the guide channel, and the limiting hole and the guide channel are arranged at an included angle of 45-75 degrees.
In a first aspect, the resilient member is a spring or a rubber band.
In a first aspect, the filter membrane replacement device comprises a stop bar; the limiting rod vertically penetrates through the top cover to the base, and the top cover can freely slide up and down along the limiting rod.
In the first aspect, the winding roll is driven by any one of the following driving means: a servo motor or a stepper motor.
Has the advantages that: the invention provides a pull-up filter membrane replacing device for environmental DNA sampling, which is characterized in that a magazine type mechanism provided with a plurality of filter membranes and a sampling assembly arranged on one side of the magazine type mechanism are arranged on a horizontal base, then filter cakes in the magazine type mechanism are conveyed to the sampling assembly one by one through a conveying assembly for water sample collection, wherein the magazine type mechanism is cylindrical, a channel through which the filter cakes can pass is arranged at the bottom of the magazine type mechanism, the collecting assembly comprises a top cover, a space is formed between the top cover and the base and is aligned with one side port of the channel, the conveying assembly comprises a sliding block and a wire winding disc, the sliding block is arranged at the other side port of the channel, the wire winding disc is arranged above the top cover and is wound with a first wire drawing, the free end of the first wire drawing is connected with the top cover, the pull-up filter membrane replacing device also comprises a second wire drawing, one end of the second wire drawing is connected with the bottom of the top cover, the other end of the second wire drawing is connected with the sliding block, when the take-up reel rotated, the pulling top cap made the top cap upwards take place the displacement, and then the pulling second wire drawing promotes the slider displacement to the passageway in, made the filter cake that is located the passageway promoted to the space between top cap and the base to carry out water sample collection. Through this kind of mode, avoided artifical contact filter cake for the operating error that leads to because the difference of manual skill, and then avoid the manual work to carry out the inefficiency that the filter membrane was changed.
Drawings
In order to more clearly illustrate the embodiments of the present disclosure or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present disclosure, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a first diagram of a drawing-up filter replacing apparatus for environmental DNA sampling according to a first embodiment of the present invention;
FIG. 2 is a second diagram of the structure of a pull-up type filter exchange device for environmental DNA sampling according to the first embodiment of the present invention.
Description of reference numerals:
1. a magazine type mechanism;
2. a base;
3. a filter cake;
4. a take-up reel;
5. first wire drawing;
6. a slider;
7. a top cover;
8. an elastic member;
9. second wire drawing;
10. a water inlet pipe;
11. a water outlet pipeline;
12. a limiting column;
13. a magazine through hole;
14. a crank lever;
15. a rotating shaft;
16. a sliding post;
17. a limiting rod;
18. a guide channel.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments that can be derived by one of ordinary skill in the art from the embodiments given herein are intended to be within the scope of the present invention.
Meanwhile, in the embodiments of the present description, when an element is referred to as being "fixed to" another element, it may be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used in the embodiments of the present specification are for illustrative purposes only and are not intended to limit the invention.
The first embodiment is as follows:
as shown in fig. 1-2, the first embodiment provides a pull-up filter membrane replacing device for environmental DNA sampling, in which a filter membrane is assembled into a filter cake 3 of an integrated structure by a ring-shaped housing, and the filter membrane replacing device includes: the device comprises a base 2, a magazine type mechanism 1, a sampling assembly and a conveying assembly;
the base 2 is a flat plate structure with a horizontal upper surface;
the magazine type mechanism 1 is cylindrical, the magazine type mechanism 1 is arranged on the base 2, a plurality of filter cakes 3 to be sampled are arranged in the magazine type mechanism 1, and a cake passage through which one filter cake 3 can pass is arranged at the bottom of the magazine type mechanism 1;
the sampling assembly is arranged at one side port of the cake passing channel on the base 2 and is used for collecting a water sample of a filter cake 3 to be sampled; the sampling assembly comprises a top cover 7, a cake containing space for clamping the filter cake 3 to sample the filter cake is formed between the top cover 7 and the base 2, the top cover is connected with an external water inlet pipeline 10 for receiving a water source to be sampled, and the base is connected with an external water outlet pipeline 11 for discharging water filtered by the filter cake;
the conveying assembly is used for conveying the filter cake 3 to the cake containing space; the conveying assembly comprises a sliding block 6 and a winding roll 4, the sliding block 6 is arranged on the base 2 and is positioned at the port of the other side of the cake passing channel, the winding roll 4 is arranged above the top cover 7, a first drawn wire 5 is wound on the winding roll 4, the free end of the first drawn wire 5 is vertically connected to the top cover 7, and the bottom of the top cover 7 is connected with the sliding block 6 through a second drawn wire 9;
when the winding roll 4 rotates, the top cover 7 is pulled through the first wire drawing 5, the top cover 7 pulls the sliding block 6 through the second wire drawing 9, the sliding block 6 penetrates through the port on the other side to push the filter cake 3 to be sampled in the cake passing channel, and the filter cake 3 penetrates through the port on one side of the cake passing channel to move to the cake containing space.
In the technical scheme of the first embodiment, a magazine type mechanism 1 provided with a plurality of filter membranes and a sampling assembly arranged on one side of the magazine type mechanism 1 are arranged on a horizontal base 2, then filter cakes 3 in the magazine type mechanism 1 are conveyed to the sampling assembly one by one through a conveying assembly for water sample collection, wherein the magazine type mechanism 1 is cylindrical, a cake passing channel through which one filter cake 3 can pass is arranged at the bottom of the magazine type mechanism, the collecting assembly comprises a top cover 7, a cake containing space is formed between the top cover 7 and the base 2 and is aligned to a port on one side of the cake passing channel, the conveying assembly comprises a sliding block 6 and a winding roll 4, the sliding block 6 is arranged at a port on the other side of the cake passing channel, the winding roll 4 is arranged above the top cover 7 and is wound with a first drawn wire 5, the free end of the first drawn wire 5 is connected with the top cover 7, the conveying assembly further comprises a second drawn wire 9, one end of the second drawn wire 9 is connected with the bottom of the top cover 7, the other end of second wire drawing 9 is connected with slider 6, and when take-up reel 4 rotated, pulling top cap 7 made top cap 7 upwards take place the displacement, and then pulling second wire drawing 9 promotes slider 6 displacement to cross the cake passageway in, makes the filter cake 3 that is located to cross the cake passageway pushed the appearance cake space between top cap 7 and the base 2 to carry out water sample collection. In this way, manual contact with the filter cake 3 is avoided, so that operation errors caused by different manual methods are avoided, and the low efficiency of manual filter membrane replacement is avoided.
Specifically, for the magazine type mechanism 1 itself, the first embodiment proposes an implementation mode, which includes: a pair of magazine through holes 13 are correspondingly arranged on a pair of opposite side walls at the lower part of the cylindrical magazine type mechanism 1, and a plurality of filter cakes 3 are arranged in the magazine type mechanism 1 in an up-and-down overlapping manner; as for the filter membrane replacing device in the first embodiment, a specific embodiment is provided in the first embodiment, in which the magazine through hole 13 of the magazine mechanism 1 is combined, and the specific embodiment includes: the limiting column 12 is vertically arranged on the top cover 7, and the upper part of the limiting column 12 is conical; the pair of crank levers 14 are made of elastic metal materials, the crank levers 14 are long rod structures, interference parts are arranged at two ends of each long rod structure in a protruding mode, the middle of each long rod structure is connected with the outer wall of the magazine type mechanism 1 through a rotating shaft 15, one interference part of each long rod structure is arranged in a magazine through hole 13 on the corresponding side and is in contact with the filter cake 3, and the interference part of the other end of each long rod structure is in contact with the upper portion of the limiting column 12; when the top cover 7 is at an initial position of initial rising, the pair of interference parts of the long rod structure correspondingly and naturally contact with the filter cake 3 and the limiting column 12, when the top cover 7 moves upwards, the conical surface of the limiting column 12 pushes the interference part at the other end of the long rod structure, the long rod structure rotates around the rotating shaft 15, so that the interference part at one end of the long rod structure is in close contact with the filter cake 3 at the corresponding side, and thus, the filter cake 3 is pushed, and the filter cake 3 is clamped by one end, which is extended into the magazine through hole 13 and is in contact with the side wall of the filter cake 3, so that the pressure of the filter cake 3 in the channel is reduced, and further, the friction force between the filter cake 3 and the base 2 is reduced when the filter cake 3 is pushed;
further, with respect to the technical solution of the first embodiment in which the power output is performed by the winding roll 4 in cooperation with the first wire 5, the first embodiment also proposes an implementation mode, which includes: a pulley block is arranged on the first drawn wire 5 to improve the stability of the first drawn wire 5 when being pulled by the winding roll 4;
after the slide block 6 in the first embodiment is pulled to push the filter cake 3 between the top cover 7 and the base 2, the first embodiment further provides an implementation manner, which includes: set up an elastic component 8 that has flexible function, the one end of elastic component 8 is connected with the one end that the slider 6 kept away from magazine-type mechanism 1, and the other end of elastic component 8 is connected with base 2, and when elastic component 8 was in the natural extension state, slider 6 was located initial movement position, and after slider 6 was promoted, elastic component 8 was elongated, and when the pulling force of slider 6 disappeared, then utilized the elasticity of elastic component 8 to make slider 6 resume to the normal position.
Further, as for the base, the first embodiment provides an implementation manner, which includes: the base is provided with a sliding groove, the direction of the sliding groove is opposite to the port of the other side, the sliding block 6 is arranged in the sliding groove and can slide along the sliding groove, and the sliding groove plays a role in guiding and limiting the direction of the sliding block 6.
Further, for the floor, the first embodiment also proposes a technical solution for improving the structure itself, and proposes a new embodiment with the technical solution and the sliding block 6, and the new embodiment comprises: a guide channel 18 penetrating through the front side and the back side of the base 2 is arranged on the base 2, the guide channel 18 is arranged in the sliding groove and is in the same direction with the sliding groove, a sliding column 16 is arranged at the bottom of the sliding block 6, and the sliding column 16 penetrates through the guide channel 18 and is positioned at the bottom of the base 2; one end of the second drawn wire 9 is connected with the sliding column 16, and the other end of the second drawn wire 9 is connected with the top cover 7 through the base 2; the elastic piece 8 is arranged on the lower surface of the base 2, one end of the elastic piece is connected with the sliding column 16, and the other end of the elastic piece is connected with the end part of the base 2 far away from the magazine type mechanism 1; this allows the slider 6 to be restored to its original position by the elastic member 8 after being pulled.
Further, as for the sliding column 16 of the first embodiment, the first embodiment further proposes an implementation manner, which includes: the cylinder body of the sliding column 16 is also provided with a through limiting hole, the second wire drawing 9 passes through the limiting hole to be connected with the elastic part 8, the setting directions of the second wire drawing 9 and the spring are consistent with the setting direction of the guide channel 18, and the limiting hole and the direction of the guide channel 18 form an included angle of 45-75 degrees.
Further, as for the elastic member 8 in the first embodiment, the first embodiment proposes an implementation mode, which includes: the elastic element 8 comprises one of the following means: springs or rubber bands.
As for the ascending direction of the top cover 7 in the first embodiment, the first embodiment further proposes an implementation manner, which includes: a stopper rod 17; the limiting rod 17 vertically penetrates through the top cover 7 to the base 2, and the top cover 7 can freely slide up and down along the limiting rod 17.
Finally, as for the driving source of the winding roll 4, the first embodiment also proposes an embodiment, which includes: a servo motor or a stepper motor.
Since the second embodiment and the first embodiment are the same in the same inventive concept, and the structure of the second embodiment is completely the same as that of the first embodiment, the detailed description of the structure of the second embodiment that is substantially the same as that of the first embodiment is omitted, and the detailed description of the structure of the second embodiment is omitted.
Finally, it should be noted that: the above-mentioned embodiments are merely specific embodiments of the present invention, which are used for illustrating the technical solutions of the present invention and not for limiting the same, and the protection scope of the present invention is not limited thereto, although the present invention is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: any person skilled in the art can modify or easily conceive the technical solutions described in the foregoing embodiments or equivalent substitutes for some technical features within the technical scope of the present disclosure; and the modifications, changes or substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention. Are intended to be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.
Claims (10)
1. A pull-up filter membrane exchange device usable for environmental DNA sampling, the filter membrane being assembled with a filter cake of an integral structure by a housing of an annular shape, the filter membrane exchange device comprising:
the base is of a flat plate structure with a horizontal upper surface;
the magazine type mechanism is arranged on the base, a plurality of filter cakes to be sampled are arranged in the magazine type mechanism, and a cake passing channel through which one filter cake can pass is arranged at the bottom of the magazine type mechanism;
the sampling assembly is arranged on the base and positioned at one side port of the cake passing channel and is used for collecting a water sample of the filter cake; the sampling assembly comprises a top cover, and a cake containing space for clamping the filter cake is formed between the top cover and the base;
the conveying assembly comprises a sliding block and a wire winding roll, the sliding block is arranged on the base and is positioned at the port on the other side of the cake passing channel, the wire winding roll is arranged above the top cover, a first wire drawing is wound on the wire winding roll, the free end of the first wire drawing is vertically connected to the top cover, and the bottom of the top cover is connected with the sliding block through a second wire drawing;
when the winding roll rotates, the top cover is pulled through the first wire drawing, the top cover pulls the sliding block through the second wire drawing, the sliding block penetrates through the port on the other side of the cake passing channel to push the filter cake to be sampled, and the filter cake penetrates through the port on one side of the cake passing channel to move to the cake containing space.
2. The pull-up filter exchange device for environmental DNA sampling according to claim 1, wherein:
a pair of magazine through holes are correspondingly formed in a pair of opposite side walls at the lower part of the cylindrical magazine type mechanism, and a plurality of filter cakes to be sampled are arranged in the magazine type mechanism in an up-and-down overlapping mode;
the filter membrane replacement device further comprises:
the limiting column is vertically arranged on the top cover, and the upper part of the limiting column is in a conical shape;
the pair of crank levers are of long rod structures, interference parts are arranged at two ends of each long rod structure, the middle of each long rod structure is connected with the outer wall of the magazine type mechanism through a rotating shaft, one interference part of each long rod structure is arranged in the corresponding magazine through hole and is in contact with the filter cake, and the other interference part of each long rod structure is in contact with the upper part of the limiting column; when the top cover is in an initial position of initial rising, the pair of interference parts of the long rod structure are correspondingly in natural contact with the filter cake and the limiting column, when the top cover is displaced upwards, the conical surface of the limiting column pushes the interference part at the other end of the long rod structure, and the long rod structure rotates around the rotating shaft, so that the interference part at one end of the long rod structure is in close contact with the filter cake at the corresponding side.
3. The pull-up filter exchange device for environmental DNA sampling according to claim 1, wherein:
and the first wire drawing is also provided with a pulley block.
4. The pull-up filter exchange device for environmental DNA sampling according to claim 1, wherein:
the filter membrane replacing device further comprises an elastic piece with a telescopic function, one end of the elastic piece is connected with one end, far away from the magazine type mechanism, of the sliding block, the other end of the elastic piece is connected with the base, and when the elastic piece is in a natural extension state, the sliding block is located at an initial moving position.
5. The pull-up filter exchange device for environmental DNA sampling according to claim 4, wherein:
the base is provided with a sliding groove, the direction of the sliding groove is opposite to the other side port, and the sliding block is arranged in the sliding groove and can slide along the sliding groove.
6. The pull-up filter exchange device for environmental DNA sampling according to claim 5, wherein:
the base is provided with a guide channel penetrating through the front side and the back side of the base, the guide channel is arranged in the sliding groove and has the same direction with the sliding groove, the bottom of the sliding block is provided with a sliding column, and the sliding column penetrates through the guide channel and is positioned at the bottom of the base;
one end of the second drawn wire is connected with the sliding column, and the other end of the second drawn wire is connected to the top cover through the base in a penetrating manner;
the elastic component sets up the lower surface of base, just the one end of elastic component with sliding column connects, the other end of elastic component with the base is kept away from the end connection of magazine-type mechanism.
7. The pull-up filter exchange device for environmental DNA sampling according to claim 6, wherein:
the cylinder body of the sliding column is also provided with a through limiting hole, the second drawn wire penetrates through the limiting hole to be connected with the elastic part, the setting directions of the second drawn wire and the spring and the guide channel are consistent, and the limiting hole and the guide channel are arranged at an included angle of 45-75 degrees.
8. The pull-up filter exchange device for environmental DNA sampling according to claim 7, wherein:
the elastic piece is a spring or a rubber band.
9. The pull-up filter exchange device for environmental DNA sampling according to claim 1, wherein:
the filter membrane replacing device comprises a limiting rod; the limiting rod vertically penetrates through the top cover to the base, and the top cover can freely slide up and down along the limiting rod.
10. The pull-up filter exchange device for environmental DNA sampling according to claim 1, wherein:
the winding roll is driven by any one of the following driving devices:
a servo motor or a stepper motor.
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| CN202210156593.5A CN114602326B (en) | 2022-02-21 | 2022-02-21 | Lifting type filter membrane replacement device capable of being used for environmental DNA sampling |
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| CN114602326B CN114602326B (en) | 2023-05-09 |
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| JP2003063647A (en) * | 2001-08-28 | 2003-03-05 | Toshiba Electric Appliance Co Ltd | Goods storing/ejecting apparatus |
| US20100288128A1 (en) * | 2009-05-13 | 2010-11-18 | Apm Engineering Co., Ltd. | Filter cassette magazine and apparatus for sequentially gathering fine particles using the same |
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| CN114602326B (en) | 2023-05-09 |
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