CN114602322B - Magazine type filter membrane replacement system capable of being used for environmental DNA sampling - Google Patents

Magazine type filter membrane replacement system capable of being used for environmental DNA sampling Download PDF

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CN114602322B
CN114602322B CN202210142650.4A CN202210142650A CN114602322B CN 114602322 B CN114602322 B CN 114602322B CN 202210142650 A CN202210142650 A CN 202210142650A CN 114602322 B CN114602322 B CN 114602322B
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filter
magazine
water
cake
top cover
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CN114602322A (en
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陆苏祥
曾宏辉
熊凡
甘小妮
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Institute of Hydrobiology of CAS
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Institute of Hydrobiology of CAS
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A20/00—Water conservation; Efficient water supply; Efficient water use
    • Y02A20/20—Controlling water pollution; Waste water treatment

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hydrology & Water Resources (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention provides a magazine type filter membrane replacement system for sampling environmental DNA, wherein a filter membrane is covered by a shell to form a filter cake with an integrated structure, and the system comprises: the device comprises a plurality of magazine-type mechanisms, sampling areas and telescopic rods, wherein a plurality of filter cakes are arranged in each magazine-type mechanism, and a channel capable of horizontally passing through one filter cake is arranged at the bottom of each magazine-type mechanism; the sampling areas are arranged on one sides of the magazine-type mechanisms and are used for correspondingly receiving the filter cakes to be sampled so as to collect water samples of the filter cakes to be sampled; the telescopic rod is positioned at the other side of the magazine type mechanism, a plurality of pushing parts are arranged at the telescopic end of the telescopic rod, each pushing part is correspondingly positioned at the inlet of one channel, and the pushing parts of the telescopic rod are used for pushing the filter cakes so as to realize the technical effect of simultaneously replacing a plurality of filter cakes.

Description

Magazine type filter membrane replacement system capable of being used for environmental DNA sampling
Technical Field
The invention belongs to the technical field of water sample collection, and particularly relates to a magazine type filter membrane replacement system capable of being used for environmental DNA sampling.
Background
Along with the advancement of new strategies for global biodiversity management and the implementation of the united nations 'biodiversity convention', the importance of biodiversity investigation and research is becoming more and more prominent. With the development of biodiversity research, large-scale and high-frequency metagenomic analysis of environmental DNA and microorganisms in water is generally required, which requires large-scale filtration of water samples and high-frequency replacement of filter membranes. Different from the common filter membrane replacement scene, in the environment DNA sampling or microorganism sampling scene, special attention is required to avoid cross contamination between samples and avoid introducing new pollution in the operation process, thereby ensuring the independence of the samples and the accuracy of detection results. In the past, the method for simply and directly replacing the filter membrane is that after a water sample is filtered by a wafer filter membrane, an experimenter manually opens the filter head and takes out the filter membrane containing the sample by using clean tweezers, then the filter head is manually reinstalled after the filter membrane is replaced by a new wafer filter membrane by using the clean tweezers. Whether applied to long-term continuous monitoring at a fixed site or to sequential sampling of multiple sites following a survey vessel, this means a large-scale high-frequency disc filter replacement operation. The major problems and disadvantages of the prior art include: due to the current manual replacement of the disc filter membrane, the operation is complicated, and different experimenters may introduce errors due to different operation methods. And the operation flow of disassembling, film taking, film changing and reinstalling is excessively consumed, so that a great deal of time and labor are wasted, and the high-frequency sampling is not facilitated. Again, manual replacement of the filters also tends to introduce contamination, causing deviations in the sample survey results. Finally, the manual filter membrane replacement mode is not separated from the operation of people, is not beneficial to automatic sampling and filtering, and is also not beneficial to standardized long-term continuous sampling or online continuous monitoring activities. The scheme of replacing the filter membranes by pneumatic adsorption used in other fields is easy to cause cross contamination between the filter membranes. The scheme of rotating disc type filter membrane replacement used in other fields has the advantages that the number of the filter membranes which can be accommodated is small, and the large-scale high-frequency application of the scene of disc filter membrane replacement cannot be met.
That is, in the prior art, the filter membrane is replaced manually, so that there are drawbacks in that the operation is complicated, errors may be introduced by different experimenters due to different operation methods, and the replacement efficiency is low.
Therefore, how to improve the replacement speed of the filter membrane and reduce the error occurring in the replacement process, so as to improve the replacement efficiency of the filter membrane is a technical problem that needs to be solved by those skilled in the art.
Disclosure of Invention
The invention provides a magazine type filter membrane replacement system for environmental DNA sampling, which at least solves the technical problems.
To solve the above problems, a first aspect of the present invention provides a cartridge-type filter membrane replacement system usable for environmental DNA sampling, the filter membrane being assembled by a housing as a filter cake of unitary structure, the system comprising: the device comprises a plurality of magazine-type mechanisms, wherein a plurality of filter cakes are arranged in each magazine-type mechanism, and a cake passing channel capable of horizontally passing through one filter cake is arranged at the bottom of each magazine-type mechanism; the sampling areas are arranged on one sides of the magazine-type mechanisms and are used for correspondingly receiving the filter cakes to be sampled so as to collect water samples of the filter cakes to be sampled; the telescopic rod is positioned at the other sides of the magazine-type mechanisms, a plurality of pushing parts are arranged at the telescopic ends of the telescopic rod, and each pushing part is correspondingly positioned at the inlet of one cake passing channel.
In the first aspect, each magazine mechanism comprises a magazine barrel, wherein a plurality of filter cakes are arranged in the magazine barrel in a stacked manner, and one filter cake naturally falls into the cake passage; the sampling area is internally provided with a bottom plate and a top cover, a plurality of water inlet pipelines for introducing sampling water are arranged on the top cover at intervals, a plurality of water outlet pipelines for discharging the water are arranged on the bottom plate at intervals, and each water inlet pipeline and one water outlet pipeline which is correspondingly arranged between the bottom plate and the top cover are correspondingly positioned at the outlet of one cake passage.
In a first aspect, a plurality of elastic members are arranged between the bottom plate and the top cover at intervals, and the elastic members are connected between the bottom plate and the top cover; one end of a pull rope is arranged on the top plate, and the other end of the pull rope penetrates through the telescopic rod and is connected to the pushing part of the telescopic rod; the filter membrane replacement system further comprises a guide member comprising: the guide ring is fixed above the top cover, the guide ring is sleeved on the pull rope, and the pulling direction of the pull rope is guided to be consistent with the vertical lifting direction of the top cover; when the telescopic rod executes the pushing action, the telescopic end pulls the pull rope, the other end of the pull rope pulls the top cover, the elastic piece is elastically deformed, a cake containing space for containing a cake is formed between the top cover and the bottom plate, meanwhile, the telescopic end stretches into the cake passing channel to push the cake in the cake passing channel to the position between the top plate and the bottom plate, and when the telescopic rod executes the resetting action, the pull rope returns to the original position, so that the elastic piece contracts, and the top cover is pressed on the cake.
In a first aspect, each of the thrusters is adapted to the shape of the outer contour of the filter cake.
In a first aspect, the filter cake has a metal layer disposed on a peripheral sidewall thereof; the inner side of the pushing part is provided with a layer of magnetic sheet.
In the first aspect, a silica gel gasket is arranged on one side of the pushing part, which is close to the filter cake.
In a first aspect, the system further comprises a water intake assembly comprising: the integrated water valve is provided with a plurality of valves with an integrated structure, and the outlet of each valve is communicated with a water inlet pipeline; the water sucking pipes are communicated with the inlets of the valves, and one end of each water sucking pipe close to the inlet of each valve is flush; the water pump is arranged on the water inlet pipeline.
In a first aspect, each of the pumping pipes is provided with a scale, and a start point of the scale is located at a flush end of the pumping pipe.
In a first aspect, the water suction tube is a telescoping tube.
In a first aspect, a pontoon is provided at the flush end of the draft tube.
The beneficial effects are that: the invention provides a magazine type filter membrane replacement system for environmental DNA sampling, which is characterized in that a plurality of magazine type mechanisms are arranged, pushing parts of telescopic rods are arranged in a plurality of ways, filter cakes in the plurality of magazine type mechanisms are correspondingly pushed to a sampling area through the plurality of pushing parts, so that the technical effect of simultaneously replacing a plurality of filter cakes is realized, the mode of manually replacing the filter membrane in the prior art is replaced by the way of automatically pushing the telescopic rods, the operation error caused by the difference of manual operation methods is solved, and the filter cake replacement efficiency and the filter cake replacement precision are improved.
Drawings
In order to more clearly illustrate the embodiments of the present description or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a block diagram of a cartridge filter replacement system for environmental DNA sampling in accordance with a first embodiment of the present invention;
FIG. 2 is a diagram showing the structural relationship between the magazine barrel, the pushing portion and the filter cake in the first embodiment of the present invention;
FIG. 3 is a schematic view showing the connection orientations of the elastic member with the top cover and the bottom plate in the first embodiment of the present invention.
Reference numerals illustrate:
1. a bottom plate;
2. a top cover;
3. a magazine mechanism;
4. a filter cake;
5. a fixing device;
6. a telescopic rod;
602. a pushing part;
7. a cake passage;
8. an elastic member;
9. a water inlet pipe;
10. and a water outlet pipeline.
Detailed Description
The following description of the embodiments of the present invention will be made more apparent and fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which are derived by a person skilled in the art based on the embodiments of the invention, fall within the scope of protection of the invention.
Meanwhile, in the embodiment of the present specification, when an element is referred to as being "fixed to" another element, it may be directly on the other element or may be present with an intervening element. When a component is considered to be "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical", "horizontal", "left", "right" and the like are used in the embodiments of the present specification for the purpose of illustration only and are not intended to limit the present invention.
Embodiment one:
as shown in fig. 1-3, a cartridge filter replacement system for sampling environmental DNA is provided, in which a filter is enclosed by a housing to form a filter cake 4, the system comprising: a plurality of magazine mechanisms 3, sampling areas and telescopic rods 6;
a plurality of filter cakes 4 are arranged in each magazine-type mechanism 3, and a cake passing channel 7 which can horizontally pass through one filter cake 4 is arranged at the bottom of each magazine-type mechanism 3;
the sampling areas are arranged on one sides of the magazine-type mechanisms 3 and are used for correspondingly receiving the filter cakes 4 to be sampled so as to collect water samples of the filter cakes 4 to be sampled;
the telescopic rod 6 is positioned at the other side of the magazine-type mechanisms 3, a plurality of pushing parts 602 are arranged at the telescopic end of the telescopic rod 6, and each pushing part 602 is correspondingly positioned at the inlet of one cake passage 7.
In the technical scheme of the first embodiment, the plurality of magazine-type mechanisms 3 are arranged, the pushing parts 602 of the telescopic rods 6 are arranged in a plurality of ways, the filter cakes 4 in the plurality of magazine-type mechanisms 3 are correspondingly pushed to the sampling area through the plurality of pushing parts 602 at the same time, so that the technical effect of simultaneously replacing the plurality of filter cakes 4 is realized, the mode of manually replacing the filter membrane in the prior art is replaced by the mode of automatically pushing the telescopic rods 6, the operation error caused by the difference of manual operation methods is solved, and the filter cake 4 replacing efficiency and the replacing precision are improved;
further, for the specific arrangement of the magazine mechanism 3 and the sampling area, the present invention provides an embodiment comprising: each magazine mechanism 3 comprises a magazine barrel 301, a plurality of filter cakes 4 are arranged in the magazine barrel 301 in a stacked manner up and down, and one filter cake 4 naturally falls into the cake passage 7; a bottom plate 1 and a top cover 2 are arranged in the sampling area, a plurality of water inlet pipelines 9 for introducing sampling water are arranged on the top cover 2 at intervals, a plurality of water outlet pipelines 10 for discharging the sampled sampling water are arranged on the bottom plate 1 at intervals, wherein each water inlet pipeline 9 is correspondingly positioned at the outlet of one cake passage 7 with a water outlet pipeline 10 correspondingly arranged between the bottom plate 1 and the top cover 2, when the filter cake 4 is pushed to the sampling area, the filter cake can move to the bottom plate 1 and the top cover 2, and suction filtration sampling and discharge are carried out through the water inlet pipelines 9 and the water outlet pipelines 10 arranged on the bottom plate 1 and the top cover 2
Meanwhile, as for the mating connection relationship between the top cover 2 and the bottom plate 1 in the above-described embodiment, the embodiment also proposes an embodiment including: a plurality of elastic pieces 8 are arranged between the bottom plate 1 and the top cover 2 at intervals, and the elastic pieces 8 are connected between the bottom plate 1 and the top cover 2; one end of a pull rope is arranged on the top plate, and the other end of the pull rope passes through the telescopic rod 6 and is connected to the pushing part 602 of the telescopic rod 6; so that the telescopic rod 6 can pull the top cover 2 to rise when pushing, and a cake containing space is reserved for water sample collection of the filter cake 4; meanwhile, in order to ensure that the direction in which the pull cord pulls the top cover 2 remains vertical, this embodiment further proposes an embodiment including: the system further includes a guide member comprising: the guide ring and the connecting rod are used for fixing the guide ring above the top cover 2, sleeving the guide ring on the pull rope and guiding the pulling direction of the pull rope to be consistent with the vertical rising direction of the top cover 2; when the telescopic rod 6 executes pushing action, the telescopic end pulls the pull rope, the other end of the pull rope pulls the top cover 2, the elastic piece 8 is elastically deformed, a cake containing space for placing the filter cake 4 is formed between the top cover 2 and the bottom plate 1, meanwhile, the telescopic end stretches into the cake passing channel 7 to push the filter cake 4 positioned in the cake passing channel 7 between the top plate and the bottom plate 1, and when the telescopic rod 6 executes resetting action, the pull rope returns to the original position, so that the elastic piece 8 is contracted, and the top cover 2 is pressed on the filter cake 4;
further, in the telescopic rod 6 technology of the first embodiment, the pushing portion 602 of the telescopic rod 6 is used to push the filter cake 4, so as to ensure the sufficient contact between the pushing portion 602 and the outer wall of the filter cake 4, the first embodiment provides an implementation manner, which includes: each pushing portion 602 is configured to be adapted to the outer contour of the filter cake 4, so that the pushing portions 602 can be fully attached to the filter cake 4 when in pushing contact.
As for the magazine 301 technology in the first embodiment, the first embodiment proposes an implementation manner, which includes: the metal layer is arranged on the peripheral side wall of the filter cake 4, and the permanent magnetic sheet is arranged on the inner side of the pushing part 602, so that the pushing part 602 can be firmly adsorbed on the outer wall of the filter cake 4 when being contacted with the metal layer of the filter cake, and the stability of the filter cake during pushing is improved.
As for the manner of fixing the magazine, the first embodiment provides an embodiment, which includes: a fixing device 5, wherein the fixing device 5 is used for fixing the magazine barrel;
also, as for the pushing portion 602 in the above-mentioned first embodiment, the first embodiment proposes an implementation manner, which includes: a silica gel gasket is arranged on one side of the pushing part 602 close to the filter cake 4, so that the pushing part 602 is more attached to the outside of the filter cake 4;
specifically, for the cartridge filter replacement system that can be used for environmental DNA sampling in the above-described embodiment one, this embodiment one also proposes an implementation manner, which includes: the integrated water valve, the water suction pipes and the water pump are arranged as a plurality of valves with integrated structures, and the outlet of each valve is communicated with a water inlet pipeline 9; each water pumping pipe is communicated with an inlet of a valve, a plurality of water pumping pipes are arranged to be of preset length, and one end of each water pumping pipe, which is close to the inlet of the valve, is flush; therefore, the end parts of the water pumping pipes far away from the leveling ends are arranged according to the length of up-down interleaving, and water samples with different water levels can be pumped through the water pumping pipes arranged in up-down interleaving; and the water pump is arranged on the water inlet pipeline 9, and the power source for water sample extraction is realized through the water pump.
Further, as for the water pumping pipe in the first embodiment, the first embodiment also proposes an implementation manner, which includes: the scale is arranged on each water pumping pipe, and the starting point of the scale is positioned at the flush end of the water pumping pipe, so that the water level can be observed through the scale.
Still further, in order to better fully perform the function of pumping water at different water levels, the first embodiment also proposes an embodiment including: the water pumping pipe is arranged as the telescopic pipe, so that the change of the pumping water level can be realized through the expansion and the contraction of the telescopic pipe.
Further, for the water inlet assembly, the first embodiment also proposes an implementation manner, which includes: the floating pontoon is arranged at the flush end of the water pumping pipe, so that the water pumping pipe and the integrated water valve can be directly placed in water, floating on water is realized through the floating pontoon, and the problem that the water pumping assembly is fixed on water manually is avoided.
With continued reference to fig. 3, fig. 3 is a schematic connection diagram of the elastic member, the top cover and the bottom plate are connected together through the elastic member, and then the top cover is pulled through the pull rope and then reset through the elastic member.
Since the second embodiment and the first embodiment are an embodiment under the same inventive concept, the partial structures thereof are completely the same, and therefore, the structure substantially the same as that of the first embodiment in the second embodiment will not be described in detail, and the detailed description will be omitted herein.
Finally, it should be noted that: the above examples are only specific embodiments of the present invention, and are not intended to limit the scope of the present invention, but it should be understood by those skilled in the art that the present invention is not limited thereto, and that the present invention is described in detail with reference to the above examples: any person skilled in the art may modify or easily conceive of the technical solution described in the foregoing embodiments, or perform equivalent substitution of some of the technical features, while remaining within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit of the corresponding technical solutions. Are intended to be encompassed within the scope of the present invention. Therefore, the protection scope of the invention is subject to the protection scope of the claims.
Although embodiments of the present invention have been disclosed above, it is not limited to the details and embodiments shown and described, it is well suited to various fields of use for which the invention would be readily apparent to those skilled in the art, and accordingly, the invention is not limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.

Claims (7)

1. A cartridge filter replacement system for environmental DNA sampling, the filter being assembled by a housing as a filter cake of unitary construction, the system comprising:
the device comprises a plurality of magazine-type mechanisms, wherein a plurality of filter cakes are arranged in each magazine-type mechanism, and a cake passing channel capable of horizontally passing through one filter cake is arranged at the bottom of each magazine-type mechanism; each magazine type mechanism comprises a magazine barrel, wherein a plurality of filter cakes are arranged in the magazine barrel in a vertically stacked manner, and one filter cake naturally falls into the cake passing channel;
the sampling areas are arranged on one sides of the magazine-type mechanisms and are used for correspondingly receiving the filter cakes to be sampled so as to collect water samples of the filter cakes to be sampled; a bottom plate and a top cover are arranged in the sampling area, a plurality of water inlet pipelines for introducing sampling water are arranged on the top cover at intervals, a plurality of water outlet pipelines for discharging the water are arranged on the bottom plate at intervals, and each water inlet pipeline and one water outlet pipeline which is correspondingly arranged between the bottom plate and the top cover are correspondingly positioned at the outlet of one cake passage;
the telescopic rod is positioned at the other sides of the magazine-type mechanisms, a plurality of pushing parts are arranged at the telescopic ends of the telescopic rod, and each pushing part is correspondingly positioned at the inlet of one cake passing channel; each pushing part is matched with the shape of the outer contour of the filter cake;
a plurality of elastic pieces are arranged between the bottom plate and the top cover at intervals, and the elastic pieces are connected between the bottom plate and the top cover;
one end of a pull rope is arranged on the top cover, and the other end of the pull rope penetrates through the telescopic rod and is connected to the pushing part of the telescopic rod;
the filter membrane replacement system further comprises a guide member comprising: the guide ring is fixed above the top cover, the guide ring is sleeved on the pull rope, and the pulling direction of the pull rope is guided to be consistent with the vertical lifting direction of the top cover;
when the telescopic rod executes the pushing action, the telescopic end pulls the pull rope, the other end of the pull rope pulls the top cover, the elastic piece is elastically deformed, a cake containing space for containing the filter cake is formed between the top cover and the bottom plate, meanwhile, the telescopic end stretches into the cake passing channel to push the filter cake in the cake passing channel to the position between the top cover and the bottom plate, and when the telescopic rod executes the resetting action, the pull rope returns to the original position, so that the elastic piece contracts, and the top cover is pressed on the filter cake.
2. The cartridge filter replacement system for environmental DNA sampling of claim 1, wherein:
a metal layer is arranged on the peripheral side wall of the filter cake;
the inner side of the pushing part is provided with a layer of magnetic sheet.
3. The cartridge filter replacement system for environmental DNA sampling of claim 1, wherein:
and a silica gel gasket is arranged on one side of the pushing part, which is close to the filter cake.
4. The system for replacing a magazine-type filter membrane for environmental DNA sampling of claim 1, further comprising a water intake assembly comprising:
the integrated water valve is provided with a plurality of valves with an integrated structure, and the outlet of each valve is communicated with a water inlet pipeline;
the water sucking pipes are communicated with the inlets of the valves, and one end of each water sucking pipe close to the inlet of each valve is flush;
the water pump is arranged on the water inlet pipeline.
5. The system for replacing a cartridge filter membrane for environmental DNA sampling of claim 4, wherein:
the water pumping pipes are each provided with a scale, and the starting points of the scales are located at the flush ends of the water pumping pipes.
6. The system for replacing a magazine type filter membrane for environmental DNA sampling of claim 5, wherein:
the water suction pipe is a telescopic pipe.
7. The system for replacing a magazine type filter membrane for environmental DNA sampling of claim 5, wherein:
a pontoon is arranged at the flush end of the water pumping pipe.
CN202210142650.4A 2022-02-16 2022-02-16 Magazine type filter membrane replacement system capable of being used for environmental DNA sampling Active CN114602322B (en)

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