CN114602324B - Clamping type filter membrane replacement system capable of being used for environmental DNA sampling - Google Patents

Clamping type filter membrane replacement system capable of being used for environmental DNA sampling Download PDF

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Publication number
CN114602324B
CN114602324B CN202210149471.3A CN202210149471A CN114602324B CN 114602324 B CN114602324 B CN 114602324B CN 202210149471 A CN202210149471 A CN 202210149471A CN 114602324 B CN114602324 B CN 114602324B
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Prior art keywords
driving device
filter
cake
clamp
filter membrane
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CN202210149471.3A
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CN114602324A (en
Inventor
陆苏祥
曾宏辉
熊凡
甘小妮
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Institute of Hydrobiology of CAS
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Institute of Hydrobiology of CAS
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D63/00Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08Flat membrane modules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D65/00Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D65/00Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
    • B01D65/10Testing of membranes or membrane apparatus; Detecting or repairing leaks
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/44Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A20/00Water conservation; Efficient water supply; Efficient water use
    • Y02A20/20Controlling water pollution; Waste water treatment

Abstract

The invention provides a clamping type filter membrane replacement system for sampling environmental DNA, wherein a filter membrane is assembled into a filter cake with an integrated structure by an annular shell, and the filter membrane replacement system comprises: the device comprises a magazine type mechanism, wherein a plurality of filter cakes to be sampled are arranged in the magazine type mechanism; the sampling area is used for receiving a filter cake to be sampled and collecting a water sample of the filter cake to be sampled; the clamping device comprises a clamp, the clamp is used for clamping a filter cake to be sampled to a sampling area for water sample collection, errors caused by different methods through manual replacement of the filter membrane are avoided, and the filter cake is clamped by the clamp, so that the filter membrane replacement precision is greatly improved.

Description

Clamping type filter membrane replacement system capable of being used for environmental DNA sampling
Technical Field
The invention belongs to the technical field of water sample collection, and particularly relates to a clamping type filter membrane replacement system capable of being used for environmental DNA sampling.
Background
Along with the advancement of new strategies for global biodiversity management and the implementation of the united nations 'biodiversity convention', the importance of biodiversity investigation and research is becoming more and more prominent. With the development of biodiversity research, large-scale and high-frequency metagenomic analysis of environmental DNA and microorganisms in water is generally required, which requires large-scale filtration of water samples and high-frequency replacement of filter membranes. Different from the common filter membrane replacement scene, in the environment DNA sampling or microorganism sampling scene, special attention is required to avoid cross contamination between samples and avoid introducing new pollution in the operation process, thereby ensuring the independence of the samples and the accuracy of detection results. In the past, the method for simply and directly replacing the filter membrane is that after a water sample is filtered by a wafer filter membrane, an experimenter manually opens the filter head and takes out the filter membrane containing the sample by using clean tweezers, then the filter head is manually reinstalled after the filter membrane is replaced by a new wafer filter membrane by using the clean tweezers. Whether applied to long-term continuous monitoring at a fixed site or to sequential sampling of multiple sites following a survey vessel, this means a large-scale high-frequency disc filter replacement operation. The major problems and disadvantages of the prior art include: due to the current manual replacement of the disc filter membrane, the operation is complicated, and different experimenters may introduce errors due to different operation methods. And the operation flow of disassembling, film taking, film changing and reinstalling is excessively consumed, so that a great deal of time and labor are wasted, and the high-frequency sampling is not facilitated. Again, manual replacement of the filters also tends to introduce contamination, causing deviations in the sample survey results. Finally, the manual filter membrane replacement mode is not separated from the operation of people, is not beneficial to automatic sampling and filtering, and is also not beneficial to standardized long-term continuous sampling or online continuous monitoring activities. The scheme of replacing the filter membranes by pneumatic adsorption used in other fields is easy to cause cross contamination between the filter membranes. The scheme of rotating disc type filter membrane replacement used in other fields has the advantages that the number of the filter membranes which can be accommodated is small, and the large-scale high-frequency application of the scene of disc filter membrane replacement cannot be met.
That is, in the prior art, the filter membrane is replaced manually, so that there are drawbacks in that the operation is complicated, errors may be introduced by different experimenters due to different operation methods, and the replacement efficiency is low.
Therefore, how to improve the replacement speed of the filter membrane and reduce the error occurring in the replacement process, so as to improve the replacement efficiency of the filter membrane is a technical problem that needs to be solved by those skilled in the art.
Disclosure of Invention
The invention provides a clamping type filter membrane replacement system for sampling environmental DNA, which at least solves the technical problems:
in order to solve the above-mentioned problems, a first aspect of the present invention provides a cartridge-type filter membrane replacement system usable for environmental DNA sampling, the filter membrane being assembled as a filter cake of unitary structure by an annular housing, the filter membrane replacement system comprising: the cartridge magazine type mechanism is internally provided with a plurality of filter cakes to be sampled; the sampling area is used for receiving the filter cake to be sampled and collecting a water sample of the filter cake to be sampled; the clamping device comprises a clamp, and the clamp is used for clamping a filter cake to be sampled to the sampling area.
In a first aspect, the sampling area comprises a semicircular base, a top cover is arranged on one side of the upper part of the base, and a cake containing space for receiving the filter cake is formed between the base and the top cover; the magazine type mechanism comprises a magazine barrel, a plurality of filter cakes to be sampled in the magazine barrel are arranged in a vertically stacked mode, the magazine barrel is positioned on the other side of the upper part of the base, and a cake passing channel capable of passing through the height of one filter cake is reserved between the magazine barrel and the upper part of the base; the clamping device further comprises a first driving device, wherein the first driving device is used for executing a rotating action, is arranged at the circle center of the base, is in driving connection with the clamp and is used for driving the clamp to rotate around the circle center; wherein, the cake containing space and the cake passing channel are both positioned on a path of the clamp rotating around the circle center; when the first driving device executes a rotation action and enables the clamp to do reciprocating rotation along the cake passing channel to the cake containing space, filter cakes to be sampled in the channel are clamped to the cake containing space one by one so as to collect water samples.
In a first aspect, the clamp comprises: the device comprises a left claw finger, a right claw finger and a second driving device, wherein the left claw finger and the right claw finger are arc-shaped, one ends of the left claw finger and the right claw finger are coupled with the second driving device through a gear set, when the second driving device is driven, the gear set drives the left claw finger and the right claw finger to open and close together at one end far away from the coupling end, and the left claw finger and the right claw finger jointly form a clamping space which is suitable for the outer edge of a filter cake.
In a first aspect, the left and right jaw fingers are each provided with an anti-slip layer on opposite sides thereof.
In a first aspect, the filter membrane replacement system comprises: the detection device is used for detecting the position of the clamp and generating position information; the control device is connected with the detection device and used for acquiring the position information; the control device is also connected with the first driving device and the second driving device; when the position information received by the control device is a preset position, the first driving device is controlled to execute the rotation action, and the second driving device is controlled to be driven.
In a first aspect, the detection means comprises a camera.
In a first aspect, the detecting device includes an angle sensor, where the angle sensor is disposed at a driving end of the first driving device, and when the first driving device performs a rotation motion, it is detected whether the rotation angle of the first driving device reaches a preset rotation angle, and if so, it is determined that the first driving device rotates to a preset position.
In a first aspect, the filter membrane replacement system comprises: and the driving end of the third driving device is connected with the clamp, and the third driving device is used for executing tangential rotation, so that the clamp rotates along the horizontal tangential direction when the third driving device executes the tangential motion.
In a first aspect, the first drive means, the second drive means and the third drive means each comprise one of the following motors: a stepper motor or a servo motor.
The beneficial effects are that: the invention provides a clamping type filter membrane replacement system for environmental DNA sampling, which is characterized in that a plurality of filter cakes are arranged in a magazine type mechanism, and then the filter cakes in the magazine type mechanism are clamped through a clamping device so as to be conveyed into a sampling area for water sample collection.
Drawings
In order to more clearly illustrate the embodiments of the present description or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a diagram showing a first embodiment of a system for replacing a membrane for sampling environmental DNA;
FIG. 2 is a diagram showing a second embodiment of a system for replacing a membrane for sampling environmental DNA;
FIG. 3 is a top view of a cartridge filter replacement system for environmental DNA sampling according to a first embodiment of the present invention;
fig. 4 is a schematic diagram of the change in position of the clamp to grip the filter cake.
Reference numerals illustrate:
1. a magazine mechanism;
101. a magazine barrel;
2. a base;
3. a clamp;
301. left paw finger;
302. a right paw finger;
4. a filter cake;
5. a first driving device;
6. a second driving device;
7. a fourth driving device;
8. a limit rod;
9. a top cover;
10. a spring;
11. a limiting piece;
12. a limit column;
13. a crank lever;
14. a water inlet pipe;
15. a water outlet pipe;
16. a magazine through hole;
17. a rotating shaft;
18. a fixing frame;
19. and a fixing frame through hole.
Detailed Description
The following description of the embodiments of the present invention will be made more apparent and fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which are derived by a person skilled in the art based on the embodiments of the invention, fall within the scope of protection of the invention.
Meanwhile, in the embodiment of the present specification, when an element is referred to as being "fixed to" another element, it may be directly on the other element or may be present with an intervening element. When a component is considered to be "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical", "horizontal", "left", "right" and the like are used in the embodiments of the present specification for the purpose of illustration only and are not intended to limit the present invention.
Embodiment one:
as shown in fig. 1 to 4, a first embodiment provides a clamping filter membrane replacement system for sampling environmental DNA, in which a filter membrane is assembled into a filter cake 4 with an integral structure by an annular housing, and the filter membrane replacement system includes: the device comprises a magazine mechanism 1, a sampling area and a clamping device;
a plurality of filter cakes 4 to be sampled are arranged in the magazine mechanism 1;
the sampling area is used for receiving the filter cake 4 to be sampled and collecting a water sample of the filter cake 4 to be sampled;
the clamping device comprises a clamp 3, and the clamp 3 is used for clamping a filter cake 4 to be sampled to a sampling area for water sample collection.
In the technical scheme of the first embodiment, the filter cakes 4 are arranged in the magazine type mechanism 1, and then the filter cakes 4 in the magazine type mechanism 1 are clamped through the clamping device so as to be conveyed into the sampling area for water sample collection, the clamping device comprises the clamp 3, the mechanization of filter membrane replacement is realized through the clamping mode of the clamp 3, and the error caused by different methods in the prior art due to manual filter membrane replacement is solved. The magazine mechanism 1 comprises a magazine barrel 101, the magazine barrel 101 is fixed through a fixing frame 18, and a fixing frame through hole 19 is formed in the side wall of the fixing frame 18.
Further, for the filter membrane replacement system in the first embodiment, the first embodiment proposes an implementation manner, which includes: the sampling area comprises a semicircular base 2, a top cover 9 is arranged on one side of the upper part of the base 2, and a cake containing space for receiving a filter cake 4 is formed between the base 2 and the top cover 9; the magazine type mechanism 1 comprises a magazine barrel 101, a plurality of filter cakes 4 to be sampled in the magazine barrel 101 are arranged in a mode of stacking up and down, the magazine barrel 101 is positioned on the other side of the base 2, and a cake passing channel capable of passing through the height of one filter cake 4 is reserved on the upper surface of the magazine barrel 101 away from the base 2; the clamping device further comprises a first driving device 5, wherein the first driving device 5 is used for executing a rotating action, and the first driving device 5 is arranged at the circle center position of the semicircular base 2 and is in driving connection with the clamp 3 and used for driving the clamp 3 to rotate around the circle center; wherein, the cake containing space and the cake passing channel are both positioned on the path of the clamp 3 rotating around the circle center; when the first driving device 5 executes a rotation action and makes the clamp 3 do reciprocating rotation along the cake passing channel to the cake containing space, the filter cakes 4 to be sampled in the cake passing channel are clamped to the cake containing space one by one so as to collect water samples.
Specifically, as for the jig 3, the present embodiment proposes an embodiment including: the left claw finger 301, the right claw finger 302 and the second driving device 6, wherein the left claw finger 301 and the right claw finger 302 are arc-shaped, one end of the left claw finger 301 and one end of the right claw finger 302 are coupled with the second driving device 6 through a gear set, and when the second driving device 6 drives, the gear set drives one end of the left claw finger 301 and one end of the right claw finger 302 far away from the coupling end to open and close together, and the left claw finger 301 and the right claw finger 302 jointly form a cake clamping space which is suitable for the outer edge of the filter cake 4; and the opening angle of the left claw finger 301 and the right claw finger 302 is larger than 90 degrees;
further, based on the basis of clamping the filter cake 4 by the clamp 3 in the first embodiment, the first embodiment proposes a technical solution of a detection assembly to improve the accuracy of clamping, where the technical solution includes: detection means for detecting the position of the jig 3 and generating position information; the control device is connected with the detection device and used for acquiring the position information; the control device is also connected with a first driving device 5 and a second driving device 6; when the position information received by the control device is the preset position, the first driving device 5 is controlled to execute the rotation action, and the second driving device 6 is controlled to drive.
Further, for the detection device, the first embodiment proposes an implementation manner, which includes: and the camera is used for acquiring the position information of the clamp 3.
Further, for the detection device, the first embodiment provides another implementation manner, which includes: the angle sensor is arranged at the driving end of the first driving device 5, and when the first driving device 5 executes a rotating action, whether the rotating angle of the first driving device 5 reaches a preset rotating angle or not is detected, and if so, the first driving device 5 is judged to rotate to a preset position;
further, as for the detection device, an embodiment of the present embodiment is also proposed, which includes detecting the positional accuracy of the jig 3 in combination with a camera and an angle sensor.
As for the filter membrane replacement system in the first embodiment, the first embodiment also proposes an implementation manner, which includes: and the driving end of the third driving device is connected with the clamp 3, and the third driving device is used for executing tangential rotation, and when the third driving device executes tangential action, the clamp 3 is enabled to rotate along the horizontal tangential direction so as to turn over the filter membrane positioned in the filter cake 4.
Specifically, as the first driving device 5, the second driving device 6, and the third driving device, as one embodiment of the first driving device 5, the second driving device 6, and the third driving device, the present embodiment proposes a solution including: the first driving device 5, the second driving device 6 and the third driving device can be stepping motors or servo motors.
Further, the first embodiment also proposes an implementation manner, which includes: the filter membrane replacement system further comprises: the spring 10, the limiting piece 11 and the limiting rod 8, the limiting rod 8 is penetrated to the base 2 by the top cover 9, the top cover 9 can slide up and down along the limiting rod 8, the spring 10 is sleeved on the limiting rod 8, the limiting piece 11 is arranged at one end of the limiting rod 8 far away from the base 2, the spring 10 is limited between the limiting piece 11 and the top cover 9, and when the spring 10 is in an automatic extension state, the top cover 9 is pressed on the base 2; the limiting column 12 is vertically arranged, and the upper part of the limiting column 12 is conical; the crank levers 13 are made of elastic metal materials, the crank levers 13 are of long rod structures, interference parts are arranged at two ends of each long rod structure in a protruding mode, the middle of each long rod structure is connected with the outer wall of the magazine type mechanism 1 through a rotating shaft 17, one end interference part of each long rod structure is in contact with the filter cake 4 and arranged in a magazine through hole 16 on the corresponding side, and the other end interference part of each long rod structure is in contact with the upper part of the limiting column 12; when the top cover 9 is in an initial position of initial rising, a pair of interference parts of the long rod structure naturally contact with the corresponding filter cakes 4 and the limiting columns 12, and when the top cover 9 is displaced upwards, the conical surface of the limiting columns 12 pushes the interference parts of the other ends of the long rod structure, and the long rod structure rotates around the rotating shaft 17, so that the interference parts of one ends of the other ends of the long rod structure are in close contact with the corresponding filter cakes 4; one end of the connecting plate is fixedly connected with the top cover 9, and the upper surface of the connecting plate is used for bearing the limit column 12; the driving end of the fourth driving device 7 is arranged on the lower surface of the connecting plate, and the fourth driving device 7 is used for executing the extending action and the contracting action; when the filter membrane is clamped by the clamp 3, the fourth driving device 7 pushes the connecting plate to move upwards, so that the connecting plate pushes the limiting column 12, and the limiting column 12 applies force to the crank lever 13, so that the crank lever 13 holds the filter cake 4 in the magazine through hole 16, the filter cake 4 in the cake passing channel is smoothly clamped, and after the clamp 3 is separated from the cake passing channel, the fourth driving mechanism drives the connecting plate to reset, so that the filter cake 4 in the magazine barrel 101 falls into the cake passing channel smoothly. Wherein, be provided with the inlet channel 14 that is used for introducing outside water source that awaits the sample on the top cap, be provided with the outlet conduit 15 that discharges that will be gathered on the base.
Finally, it should be noted that: the above examples are only specific embodiments of the present invention, and are not intended to limit the scope of the present invention, but it should be understood by those skilled in the art that the present invention is not limited thereto, and that the present invention is described in detail with reference to the above examples: any person skilled in the art may modify or easily conceive of the technical solution described in the foregoing embodiments, or perform equivalent substitution of some of the technical features, while remaining within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit of the corresponding technical solutions. Are intended to be encompassed within the scope of the present invention. Therefore, the protection scope of the invention is subject to the protection scope of the claims.
Although embodiments of the present invention have been disclosed above, it is not limited to the details and embodiments shown and described, it is well suited to various fields of use for which the invention would be readily apparent to those skilled in the art, and accordingly, the invention is not limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.

Claims (8)

1. A clamping filter membrane replacement system for environmental DNA sampling, the filter membrane being assembled as a filter cake of unitary construction by an annular housing, the filter membrane replacement system comprising:
the cartridge magazine type mechanism is internally provided with a plurality of filter cakes to be sampled;
the sampling area is used for receiving the filter cake to be sampled and collecting a water sample of the filter cake to be sampled; the sampling area comprises a semicircular base, a top cover is arranged on one side of the upper part of the base, and a cake containing space for receiving the filter cake is formed between the base and the top cover;
the magazine type mechanism comprises a magazine barrel, a plurality of filter cakes to be sampled in the magazine barrel are arranged in a vertically stacked mode, the magazine barrel is positioned on the other side of the upper part of the base, and a cake passing channel capable of passing through the height of one filter cake is reserved between the magazine barrel and the upper part of the base;
the clamping device comprises a clamp which is used for clamping a filter cake to be sampled to the sampling area;
the clamping device further comprises a first driving device, wherein the first driving device is used for executing a rotating action, is arranged at the circle center of the base, is in driving connection with the clamp and is used for driving the clamp to rotate around the circle center;
wherein, the cake containing space and the cake passing channel are both positioned on a path of the clamp rotating around the circle center; when the first driving device executes a rotating action and enables the clamp to do reciprocating rotation along the cake passing channel to the cake containing space, the filter cakes to be sampled in the cake passing channel are clamped to the cake containing space one by one so as to collect water samples;
the filter membrane replacement system further comprises: the limiting column is vertically arranged, and the upper part of the limiting column is conical; the crank lever is made of elastic metal, the crank lever is of a long rod structure, two ends of the long rod structure are provided with interference parts in a protruding mode, the middle of the long rod structure is connected with the outer wall of the magazine type mechanism through a rotating shaft, one end interference part of the long rod structure is arranged in a magazine through hole on the corresponding side and is in contact with a filter cake, and the other end interference part of the long rod structure is in contact with the upper part of the limit post; when the top cover is in an initial position of initial rising, a pair of interference parts of the long rod structure naturally contact with the corresponding filter cakes and the limiting columns, and when the top cover is displaced upwards, the conical surface of the limiting columns pushes the interference parts of the other ends of the long rod structure, and the long rod structure rotates around the rotating shaft, so that the interference parts of the other ends of the long rod structure are in close contact with the filter cakes on the corresponding sides; one end of the connecting plate is fixedly connected with the top cover, and the upper surface of the connecting plate is used for bearing the limit column; and the driving end of the fourth driving device is arranged on the lower surface of the connecting plate, and the fourth driving device is used for executing the extending action and the contracting action.
2. The system for changing a filter membrane for environmental DNA sampling of claim 1, wherein said clamp comprises:
the device comprises a left claw finger, a right claw finger and a second driving device, wherein the left claw finger and the right claw finger are arc-shaped, one ends of the left claw finger and the right claw finger are coupled with the second driving device through a gear set, when the second driving device is driven, the gear set drives the left claw finger and the right claw finger to open and close together at one end far away from the coupling end, and the left claw finger and the right claw finger jointly form a clamping space which is suitable for the outer edge of a filter cake.
3. The system for changing a filter membrane for environmental DNA sampling of claim 2, wherein:
the left claw finger and the right claw finger are both provided with anti-slip layers on opposite sides.
4. The system of claim 2, wherein the filter exchange system comprises:
the detection device is used for detecting the position of the clamp and generating position information;
the control device is connected with the detection device and used for acquiring the position information; the control device is also connected with the first driving device and the second driving device;
when the position information received by the control device is a preset position, the first driving device is controlled to execute the rotation action, and the second driving device is controlled to be driven.
5. The system for changing a filter membrane for environmental DNA sampling of claim 4, wherein:
the detection device comprises a camera.
6. The system for changing a filter membrane for environmental DNA sampling of claim 4, wherein:
the detection device comprises an angle sensor, the angle sensor is arranged at the driving end of the first driving device, when the first driving device executes a rotation action, whether the rotation angle of the first driving device reaches a preset rotation angle is detected, and if yes, the first driving device is judged to rotate to a preset position.
7. The system of claim 2, wherein the filter exchange system comprises:
and the driving end of the third driving device is connected with the clamp, and the third driving device is used for executing tangential rotation, so that the clamp rotates along the horizontal tangential direction when the third driving device executes the tangential rotation.
8. The system of claim 7, wherein the first drive, the second drive, and the third drive each comprise one of the following motors:
a stepper motor or a servo motor.
CN202210149471.3A 2022-02-18 2022-02-18 Clamping type filter membrane replacement system capable of being used for environmental DNA sampling Active CN114602324B (en)

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Citations (2)

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