CN114601846A - Complex vitamin amino acid injection for livestock and preparation method and application thereof - Google Patents
Complex vitamin amino acid injection for livestock and preparation method and application thereof Download PDFInfo
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- CN114601846A CN114601846A CN202011412495.0A CN202011412495A CN114601846A CN 114601846 A CN114601846 A CN 114601846A CN 202011412495 A CN202011412495 A CN 202011412495A CN 114601846 A CN114601846 A CN 114601846A
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- amino acid
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Abstract
The invention belongs to the technical field of animal husbandry and veterinary medicine, and discloses a compound vitamin amino acid injection for livestock and a preparation method and application thereof. The preparation process is simple, and the injection prepared by the improved oil-in-water process has high clarity and stable property, is suitable for vitamin and amino acid deficiency diseases of cattle, dogs and the like, and is beneficial to enhancing the animal constitution and assisting in treating parvovirus infection. The invention solves the problems of unstable property and common effect of the injection of the existing compound amino acid injection by adjusting the component proportion of the raw materials and adding vitamins, amino acid and other auxiliary substances in a targeted manner. The product of the invention is suitable for preventing and treating diseases of livestock.
Description
Technical Field
The invention belongs to the technical field of livestock and veterinary medicine, and relates to an injection for livestock and a preparation method and application thereof, in particular to a compound vitamin amino acid injection for livestock and a preparation method and application thereof.
Background
The deficiency of amino acid, vitamin and the like of animals easily causes the animal dysplasia, slow metabolism, loose and soft bones, weakness and sickliness, and even further influences the body to absorb other nutrient substances. If the symptoms are not improved in time, the animals grow slowly and even die from diseases, and further more economic loss is brought to the breeding industry.
At present, in the process of animal breeding, timely supplementing insufficient nutrient components obtained from feed by animals through medicine injection or feeding is an effective means for enhancing the physique of the animals and improving the production benefit. However, the existing injection for enhancing animal performance mainly uses glucose injection, sodium chloride injection and glucose and sodium chloride injection, can adjust the balance of electrolytes in an organism, but cannot improve the condition of animal organism nutrient deficiency, and cannot supplement various amino acids and vitamins required in the animal organism. The existing compound amino acid vitamin injection has complex components, particularly fat-soluble vitamins are easy to separate out, and a water phase and an oil phase are layered, so that the injection is turbid in state and poor in stability, and is not suitable for long-term storage.
In order to solve the problems, the invention provides the complex vitamin amino acid injection which has comprehensive nutrition supplement, high clarity and stable property, is beneficial to enhancing the physique of animals and is suitable for long-term storage, and needs to adjust the components and the proportion of the complex vitamin amino acid preparation, improve the preparation method and promote the dissolution of fat-soluble components.
Disclosure of Invention
In order to solve the defects in the prior art, the invention aims to provide the compound vitamin amino acid injection for the livestock, so as to achieve the purposes of supplementing more comprehensive nutrition, improving the clarity and stability of the injection, enhancing the physique of the animals and storing for a long time;
the invention also aims to provide a preparation method of the compound vitamin amino acid injection for livestock;
the invention also aims to provide an application of the compound vitamin amino acid injection for livestock.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a compound vitamin amino acid injection for livestock is prepared from the following raw materials of active ingredients in 1000 parts by volume:
is prepared from vitamin B hydrochloride11 to 10 parts by weight0.1 to 5 weight portions of riboflavin sodium phosphate and vitamin B60.7-5 parts by weight, 4-15 parts by weight of nicotinamide, 0.5-5 parts by weight of vitamin C, 1-10 parts by weight of dexpanthenol and vitamin B120.04-0.1 weight part of water-soluble vitamin and 0.05-0.1 weight part of biotin;
② fat-soluble vitamin which is composed of 0.8-10 parts of vitamin A oil, 0.5-7 parts of vitamin D oil and 0.5-5 parts of vitamin E oil;
③ the compound amino acid which consists of 0.3 to 5 weight portions of lysine hydrochloride, 0.6 to 4 weight portions of arginine hydrochloride, 0.4 to 2 weight portions of leucine, 0.4 to 2 weight portions of isoleucine, 0.8 to 1 weight portion of tyrosine and 0.5 to 1 weight portion of tryptophan;
fourthly, auxiliary materials consisting of 0.05 to 0.5 weight portion of antioxidant, 0.05 to 0.5 weight portion of complexing agent, 20 to 250 weight portions of solubilizer and 20 to 200 volume portions of auxiliary emulsion;
wherein the proportion of the parts by weight to the parts by volume is kg: and L.
The antioxidant is at least one of tert-butyl hydroxy anisole, dibutyl hydroxy toluene and tert-butyl hydroquinone.
As a second limitation of the present invention, the complexing agent is at least one of disodium edetate, sodium calcium edetate and gentioinolamine.
As a third definition of the present invention, the solubilizing agent is at least one of Tween-80, Tween-20, Poloxamer-68, lecithin, RH-40 and EL-35.
As a fourth limitation of the present invention, the co-emulsion is at least one of propylene glycol, polyethylene glycol-300, polyethylene glycol-400 and glycerol.
The invention also provides a preparation method of the compound vitamin amino acid injection for livestock, which is sequentially carried out according to the following steps:
I. heating water for injection, adding complexing agent and compound amino acid, mixing, cooling, adding water soluble vitamin, dissolving, and mixing to obtain water phase solution A;
II, mixing the antioxidant, the auxiliary emulsion and the solubilizer uniformly, heating, dissolving, mixing uniformly, adding the fat-soluble vitamin, and mixing uniformly to obtain an oil-phase solution B;
III, pouring the solution B into the solution A at the speed of 300-; and (3) fixing the volume, adjusting the pH value, filtering and sterilizing to obtain the compound vitamin amino acid injection for the livestock.
Adding a complexing agent and amino acid to obtain a good dissolving effect at a high temperature; then water-soluble vitamins are added to dissolve in warm water, so that the effects of difficult decomposition and stable content in long-term storage are achieved.
The preparation method is an improved oil-in-water preparation method.
As a further limitation of the invention, in the step I, the heating is to raise the temperature to 80-90 ℃, and the cooling is to lower the temperature to 40-50 ℃; in step II, the heating is carried out so that the temperature rises to 50-60 ℃.
As still further defined in the present invention, in step III, the pH is adjusted to 4-6; the filtration comprises coarse filtration and fine filtration, wherein the coarse filtration is filtration after activated carbon adsorption, and the fine filtration is filtration by a filter membrane.
The activated carbon adsorption is carried out by 0.01-0.2 percent (mass of activated carbon/volume of injection); the fine filtration is carried out by a microporous membrane with the aperture of 0.45um or 0.22 um.
The activated carbon adsorption realizes decolorization and impurity adsorption, and improves clarity and stability of the injection.
The invention also provides an application of the livestock compound vitamin amino acid injection, and the livestock compound vitamin amino acid injection is used as an livestock drug to enhance the animal constitution and treat vitamin and amino acid deficiency.
Due to the adoption of the technical scheme, compared with the prior art, the invention has the following beneficial effects:
(1) according to the livestock multivitamin amino acid injection, the water-in-water oil preparation method is adopted for improving water-soluble vitamins, fat-soluble vitamins and various amino acids, the amino acids and the vitamins in the livestock multivitamin amino acid injection are interacted after entering an animal body, the vitamins can promote the absorption of the amino acids, and the amino acids can promote the vitamins to play a greater medicinal value, so that the nutrition is better supplemented, the metabolic level of animals is improved, the blood flow running is accelerated, the body recovery is promoted, the physique of the animals is enhanced, and the production performance is improved;
(2) the compound vitamin amino acid injection for livestock of the invention is added with a plurality of amino acids, has good protection effect on liver: wherein leucine and isoleucine are branched chain amino acids, can improve the unbalance of branched chain amino acids and aromatic amino acids of the organism, prevent the hepatic coma of animals caused by overhigh concentration of the aromatic amino acids in the brain, and can be oxidized into energy sources at the periphery, so that the energy intake is improved under the condition of not increasing the burden of the liver;
(3) the livestock multivitamin amino acid injection is added with leucine and isoleucine as substrates of gluconeogenesis, the substrates are oxidized in vivo to form cyclic metabolism with alanine in raw materials, more heat is generated, and the amino acid is used as a protein synthesis raw material to directly influence the metabolic level and the production performance of animals;
(4) the livestock compound vitamin amino acid injection is added with tryptophan, and through the interaction of the tryptophan and the added riboflavin sodium phosphate, the synthesis of nicotinic acid and heme is promoted, the antibody expression level in the newborn of the pregnant animals is obviously improved, and meanwhile, the livestock compound vitamin amino acid injection has the function of promoting lactation of dairy cattle in the lactation period;
(5) the compound vitamin amino acid injection for livestock is added with a plurality of vitamins participating in the amino acid metabolic process, and the raw material proportion is reasonable, so that the injection has the effects of increasing appetite, maintaining the nerve function and promoting growth and development;
(6) according to the preparation method of the compound vitamin amino acid injection for the livestock, disclosed by the invention, an oil-in-water process is improved, the water phase is prepared in the step I, the oil phase is prepared in the step II, the oil phase is slowly added into the water phase in the step III, and the two phases are fully and uniformly mixed by stirring at a high speed while adding, so that a clear nano-emulsion preparation is formed; the mixing temperature of the oil phase and the water phase of the fat-soluble vitamin is adjusted, the fat-soluble vitamin is emulsified and dispersed in the water for injection by heating and cooling for several times and adjusting the adding sequence of the components, so that a good nano-scale oil-in-water emulsification system is formed, the solubility of the fat-soluble vitamin is improved, and the mixed system has high clarity and stable property and is suitable for long-term storage.
(7) According to the preparation method of the compound vitamin amino acid injection for livestock, the clarity is improved and the stable properties of the amino acid and the vitamin are ensured by adjusting the pH value, so that the compound vitamin amino acid injection is suitable for long-term storage; step I, adding a complexing agent and amino acid to achieve a good dissolving effect at high temperature; then adding water-soluble vitamins to dissolve in warm water, so as to achieve the effects of difficult decomposition and stable content in long-term storage;
(8) the preparation method of the compound vitamin amino acid injection for livestock adopts a coarse filtration and fine filtration double filtration method, ensures the components of the injection to be stable, and simultaneously decolorizes and removes microorganisms and impurities, so that the produced injection has safety guarantee;
in conclusion, the compound vitamin amino acid injection for livestock and the preparation method and application thereof can improve the conditions of incomplete nutrition supplement, poor clarity and poor stability of the existing amino acid injection; the preparation method is easy to control and suitable for industrial production; the preparation method is suitable for preparing the compound vitamin amino acid injection for the livestock, and the prepared compound vitamin amino acid injection for the livestock is used as a medicament for the livestock to enhance the physique of the animals and treat vitamin and amino acid deficiency.
Detailed Description
The present invention is further illustrated by the following specific examples, which are to be construed as merely illustrative, and not limitative of the remainder of the disclosure.
Example 1A method for preparing a multivitamin amino acid injection for livestock
The preparation of the complex vitamin amino acid injection for livestock comprises the following steps in sequence:
I. get notesHeating water for injection to 85 deg.C, adding disodium edetate 0.5kg, adding lysine hydrochloride 5kg, arginine hydrochloride 1kg, leucine 2kg, isoleucine 2kg, aminobutyric acid 1kg and tryptophan 1kg, stirring for dissolving, mixing, cooling to 40 deg.C, adding vitamin B hydrochloride110kg, riboflavin sodium phosphate 1kg, vitamin B65kg, 15kg of nicotinamide, 5kg of vitamin C, 10kg of dexpanthenol and vitamin B120.1kg of biotin and 0.1kg of biotin are dissolved and mixed evenly to obtain a solution A;
II, putting 0.5kg of butylated hydroxytoluene, 50L of propylene glycol and 6850 kg of poloxamer into a liquid preparation tank, uniformly mixing, heating to 60 ℃ to dissolve solids, uniformly stirring, adding 10kg of vitamin A oil, 5kg of vitamin D oil and 5kg of vitamin E oil, and uniformly stirring to obtain a solution B;
III, pouring the solution B into the solution A at a speed of 400L/h, stirring at a speed of 1500r/min while adding, and uniformly mixing to form an oil-in-water nano-emulsion solution; metering volume to 1000L, adjusting pH to 4.0, adding 1kg of active carbon, stirring, filtering with 1um sand filter stick after 30 min to remove active carbon, fine-filtering with microporous membrane with pore size of 0.45um, filtering, and sterilizing with 100 deg.C flowing steam for 30 min to obtain compound vitamin amino acid injection (labeled as M1) for livestock.
Example 2-6A method for preparing a complex vitamin amino acid injection for livestock
Examples 2 to 6 are respectively a method for preparing a complex vitamin amino acid injection for livestock, the steps of which are substantially the same as those of example 1, except for the differences in raw material dosage and process parameters, and are specifically shown in tables 1 and 2:
table 1 examples 2-6 raw material usage meter
Table 2 table of process parameters for examples 2-6
Example 7 quality control of a multivitamin amino acid injection for livestock
According to the requirements related to veterinary drug research technical guidelines (pharmaceutical section), the quality of the livestock complex vitamin amino acid injection M1-M6 prepared in examples 1-6 is tested according to the veterinary drug stability test technical specifications (trial) in the veterinary drug test technical specification compilation, and specifically comprises the following steps: detecting the content of nutrient components, observing the color and properties of the injection, and measuring the pH value and sterilization condition of the injection, wherein the corresponding labeled amount of vitamins and amino acids is controlled to be 90.0-110.0% according to the quality standard of 'drug Standard of State food and drug administration' WS1- (X-324) -2003Z, and the labeled amount is calculated according to the content of raw materials in the obtained solution/the corresponding amount of the raw materials, and the specific experimental results are shown in Table 3:
TABLE 3 quality test results of vitamin complex amino acid injection M1-M6 for livestock
According to the experimental results, the quality of the compound vitamin amino acid injection M1-M6 for livestock prepared in the examples 1-6 reaches the standard: the contents of vitamins and amino acids meet the standard, the color is yellow clear liquid, the clarity is high, the pH value is stable at 4.0-6.0, and the sterilization condition is qualified.
Example 8 stability assay of a Complex vitamin amino acid injection for livestock
The stability tests of the livestock complex vitamin amino acid injection M1-M6 prepared in the examples 1-6 and a certain commercially available livestock vitamin amino acid injection are carried out, and the test is specifically carried out by an accelerated test, and the method and the results are as follows:
strictly in accordance with the guidelines (pharmaceutical part) of veterinary drug research techniques, the method comprises placing a livestock complex vitamin amino acid injection M1-M6 and a commercially available vitamin amino acid injection for veterinary use prepared in examples 1-6 according to the technical specifications (trial) of veterinary drug stability tests in the compilation of veterinary drug test technical specifications in a microcomputer constant temperature and humidity chamber, adjusting the temperature to 40 + -2 deg.C and the relative humidity to 75 + -5%, standing for 6 months, sampling and detecting at the beginning, at the end of 1, 2, 3 and 6 months, wherein the standard pH value of the injection during the standing process is 4-6, the labeled amount of vitamins and amino acids is controlled to 90.0-110.0% according to the quality standard WS1- (X-324) -2003Z of the State food and drug administration (national food and drug administration), the calculation of the marked amount is calculated according to the content of the raw materials in the obtained solution/the corresponding raw material using amount, and specific detection data are shown in tables 4-10:
TABLE 4 stability test results of vitamin A complex amino acid injection M1 for livestock
TABLE 5 stability test results of vitamin A complex amino acid injection M2 for livestock
TABLE 6 stability test results of M3 for compound vitamin amino acid injection for livestock
TABLE 7 stability test results of M4 for compound vitamin amino acid injection for livestock
TABLE 8 stability test results of M5 for compound vitamin amino acid injection for livestock
TABLE 9 stability test results of vitamin amino acid complex injection M6 for livestock
TABLE 10 stability test results for a commercially available vitamin amino acid injection
As can be seen from tables 4 to 10, the vitamin amino acid complex injection M1-M6 for livestock prepared in examples 1 to 6 has no obvious change in each index through an accelerated test, and has good stability in color property and nutrient content; and the stability of the commercially available vitamin amino acid injection is poor, and particularly the content of fat-soluble vitamins is unstable and turbidity appears after 3 months.
Example 9 application of complex vitamin amino acid injection for livestock
Test one: experiment for effect of compound vitamin amino acid injection for livestock on promoting growth and development of sick cows
The test is carried out in a large-scale cow farm, the cows have symptoms of anorexia, emaciation, general weakness, milk yield reduction and the like, and the test is combined with clinical symptoms, local veterinarians confirm that the cows have vitamin and amino acid deficiency, 35 Chinese Holstein cows are selected and randomly divided into 7 groups, 5 groups of the cows are respectively a control group and an MA1-MA6 group, wherein the control group is given 5 percent of glucose injection intravenous injection, the MA1-MA6 group is correspondingly given one intravenous injection of compound vitamin amino acid injection M1-M6 for livestock one by one, the injection is continuously carried out for 14 days, the average weight increase value after 14 days, the average feed intake when starting and after 14 days, the average milk yield when starting and after 14 days, the milk fat, the milk protein and the milk lactose content in the milk when starting and after 14 days are recorded in the test period, and the specific results are shown in the following table:
TABLE 10 application effect of a compound vitamin amino acid injection for livestock on deficiency of vitamins and amino acids in milk cow
As can be seen from Table 10, the average weight gain of the MA1-MA6 group is increased by more than 0.95kg compared with the control group, the average feed intake of the MA1-MA6 group is increased by more than 1.9kg/d compared with the control group, the milk yield of the MA1-MA6 group is increased by more than 5.21kg/d compared with the control group, the milk fat content of the MA1-MA6 group is increased by more than 0.51 percent compared with the control group, the milk protein content of the MA1-MA6 group is increased by more than 0.3 percent compared with the control group, and the lactose content of the MA1-MA6 group is increased by more than 0.7 percent compared with the control group.
And (2) test II: experiment for promoting recovery effect of parvovirus infected dogs by using compound vitamin amino acid injection for livestock
56 parvovirus infected dogs are selected from clinical treatment dogs in a pet hospital and randomly divided into 7 groups, wherein each group comprises 8 dogs and is respectively a control group and an MB1-MB6 group, wherein the control group is administered with vitamin C injection, sodium chloride injection, canine parvovirus monoclonal antibody and the like, and the administration scheme refers to the use instruction of the medicament; the livestock compound vitamin amino acid injection M1-M6 is applied to MB1-MB6 groups in a one-to-one correspondence mode, the injection is continuously carried out for 7 days, the average body weight, the average rectal temperature, the respiratory frequency and the pulse rate of a sick dog before and after the test are observed and recorded in the test period, the biochemical indexes for representing the functions of the liver and the kidney are detected, and partial results are shown in the following table:
TABLE 11 Experimental results of the effect of vitamin complex amino acid injection for livestock in promoting recovery of parvovirus infected dogs
The results show that the average body weight, the average rectal temperature, the respiratory rate and the pulse rate of the sick dogs are not obviously influenced during the administration period and after the withdrawal of the medicine, and the biochemical indexes for representing the functions of the liver and the kidney are not obviously changed, which shows that the complex vitamin amino acid injection for livestock is safe and nontoxic; comparing the physiological indexes such as average body weight, average rectal temperature, respiratory rate, pulse rate and the like of the control group and the MB1-MB6 group, the body state of sick dogs of the MB1-MB6 group is better, and the complex vitamin amino acid injection for livestock has a promoting effect on recovery of the dogs infected with parvovirus.
Although the present invention has been described in detail with reference to the above embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described above, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.
Claims (9)
1. The compound vitamin amino acid injection for livestock is characterized in that the raw materials for preparing the effective components of the compound vitamin amino acid injection in each 1000 volume parts comprise:
wherein the proportion of the parts by weight to the parts by volume is kg: and L.
2. The complex vitamin amino acid injection for livestock according to claim 1, wherein the antioxidant is at least one of tert-butyl hydroxy anisole, dibutyl hydroxy toluene and tert-butyl hydroquinone.
3. The complex vitamin amino acid injection for livestock according to claim 1 or 2, wherein the complexing agent is at least one of disodium edetate, sodium calcium edetate and gentioinolamine.
4. The vitamin-amino acid complex injection for livestock according to claim 1 or 2, wherein the solubilizer is at least one of tween-80, tween-20, poloxamer-68, lecithin, RH-40 and EL-35.
5. The complex vitamin amino acid injection for livestock according to claim 1 or 2, wherein the co-emulsion is at least one of propylene glycol, polyethylene glycol-300, polyethylene glycol-400 and glycerol.
6. The process for preparing a multi-vitamin amino acid injection for livestock as set forth in any one of claims 1 to 5, which comprises the following steps in sequence:
7. The method for preparing the complex vitamin amino acid injection for livestock according to claim 6, which comprises the following steps
Heating to raise the temperature to 80-90 ℃, and cooling to lower the temperature to 40-50 ℃; step (ii) of
Wherein the heating is carried out such that the temperature is raised to 50-60 ℃.
8. The method for preparing the complex vitamin amino acid injection for livestock according to claim 6 or 7, which is characterized by comprising the following steps
Adjusting the pH value to 4-6; the filtration comprises coarse filtration and fine filtration, wherein the coarse filtration is filtration after activated carbon adsorption, and the fine filtration is filtration by a filter membrane.
9. The use of a livestock complex vitamin amino acid injection as claimed in any of claims 1-5, wherein the livestock complex vitamin amino acid injection is used as livestock drug for improving animal body constitution and treating vitamin and amino acid deficiency.
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