CN112472733B - A flos Puerariae Lobatae extract for promoting calcium oral absorption - Google Patents

A flos Puerariae Lobatae extract for promoting calcium oral absorption Download PDF

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CN112472733B
CN112472733B CN202011479897.2A CN202011479897A CN112472733B CN 112472733 B CN112472733 B CN 112472733B CN 202011479897 A CN202011479897 A CN 202011479897A CN 112472733 B CN112472733 B CN 112472733B
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calcium
composition
flower extract
extract
pueraria flower
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CN112472733A (en
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庄瑞宾
王建智
陈旭
侯龙辉
叶梓
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Yijia Yuanpin Industrial Xiamen Co ltd
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Yijia Yuanpin Industrial Xiamen Co ltd
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    • A61K31/59—Compounds containing 9, 10- seco- cyclopenta[a]hydrophenanthrene ring systems
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Abstract

The invention relates to a pueraria flower extract for promoting calcium oral absorption. Specifically, the invention provides a pueraria flower extract, which is prepared by the following method: (1) extracting flos Puerariae Lobatae with alcohol solvent to obtain alcohol extractive solution; (2) concentrating the alcohol extract, adding an organic solvent for extraction, and carrying out vacuum distillation on the organic phase to remove the solvent to obtain the pueraria flower extract, wherein the organic solvent comprises one or more of petroleum ether, chloroform and ethyl acetate. The pueraria flower extract can effectively promote the oral absorption of calcium, improve the level of blood calcium and treat calcium deficiency.

Description

A flos Puerariae Lobatae extract for promoting calcium oral absorption
Technical Field
The invention relates to the field of food and medicine, in particular to a pueraria flower extract for promoting calcium oral absorption.
Background
Calcium is an important structural component in the living body and is involved in regulating various physiological processes of cells, for example, physiological functions of calcium in the human body including biological calcification, blood coagulation, muscle contraction, nerve signal transmission, etc., thereby maintaining normal physiological functions.
Calcium deficiency can cause various diseases, especially for infants, children and pregnant women, and often causes osteomalacia, convulsion, waist soreness and backache, arthralgia and the like. Oral calcium tablets such as calcium carbonate tablets are a common calcium supplement method, however, the oral calcium tablets often have the problem of low absorption efficiency, especially for the elderly, so that the calcium tablets need to be taken for a long time in large quantity to supplement calcium, which results in low patient compliance, and in addition, for patients with serious calcium deficiency, the oral calcium tablets often need to be injected to supplement calcium due to slow absorption rate, but the injection calcium supplement cost is high, the patient compliance is low, and the oral calcium tablets are not suitable for the patients with long-term calcium supplement. The prior art lacks medicines for promoting the oral absorption of calcium, thereby limiting the effective treatment of calcium deficiency by oral calcium tablets.
Therefore, there is a need in the art to develop a method of promoting oral absorption of calcium to improve calcium absorption and treat calcium deficiency.
Disclosure of Invention
The invention aims to provide a pueraria flower extract which can effectively promote the oral absorption of calcium, improve the level of blood calcium and treat calcium deficiency.
The invention provides a pueraria flower extract, which is prepared by the following steps:
(1) extracting flos Puerariae Lobatae with alcohol solvent to obtain alcohol extractive solution;
(2) concentrating the alcohol extract, adding an organic solvent for extraction, and carrying out vacuum distillation on the organic phase to remove the solvent to obtain the pueraria flower extract, wherein the organic solvent comprises one or more of petroleum ether, chloroform and ethyl acetate.
Preferably, the pueraria flower extract comprises a dry extract.
Preferably, the pueraria flower extract is a dry extract.
Preferably, in the step (1), the alcohol solvent is a C1-C4 lower alcohol or a C1-C4 lower alcohol aqueous solution.
Preferably, the C1-C4 lower alcohol comprises one or more of methanol, ethanol, propanol and butanol.
Preferably, the volume fraction of the C1-C4 lower alcohol in the C1-C4 lower alcohol aqueous solution is 40-70%, preferably 50-60%, more preferably 55-65%.
Preferably, in the step (2), the organic solvent includes petroleum ether.
Preferably, the pueraria flower extract is prepared by the following method:
adding 55-65% (v/v) ethanol water solution 6-10 times the amount of flos Puerariae Lobatae, heating and reflux extracting for 4-8 hr, filtering, concentrating the filtrate under reduced pressure to obtain concentrated solution, adding petroleum ether into the concentrated solution for extraction, distilling the obtained petroleum ether organic phase layer under reduced pressure to remove solvent, and drying to obtain flos Puerariae Lobatae extract.
Preferably, the 55-65% (v/v) ethanol aqueous solution is 60% (v/v) ethanol aqueous solution.
In a second aspect of the present invention, there is provided a use of the pueraria flower extract according to the first aspect of the present invention for preparing a composition for promoting oral absorption of calcium.
Preferably, the composition is a pharmaceutical composition, a food composition or a health care composition.
Preferably, the composition further comprises a pharmaceutically, food or nutraceutical acceptable carrier.
Preferably, the composition is in a solid or liquid form.
Preferably, the composition is in the form of oral preparation or injection preparation.
Preferably, the calcium comprises calcium from calcium carbonate.
Preferably, the food composition is a beverage or yogurt.
Preferably, the composition is in the form of emulsion, tablet, capsule, oral liquid, granule, powder or syrup.
Preferably, the composition further comprises vitamin D.
Preferably, said vitamin D comprises vitamin D3.
Preferably, the ratio (mg: IU) of the pueraria flower extract to the vitamin D is (1-40): (80-320), preferably (5-20): (100- & ltSUB & gt 300) ], more preferably (5-15): (150-250), more preferably (8-12): (180-220).
Preferably, the composition further comprises L-arabinose.
In a third aspect, the present invention provides a composition comprising a pueraria flower extract according to the first aspect of the present invention and vitamin D.
Preferably, said vitamin D comprises vitamin D3.
Preferably, the composition further comprises L-arabinose.
Preferably, the composition further comprises calcium carbonate.
Preferably, the composition is a pharmaceutical composition, a food composition, a nutraceutical composition or a beverage composition.
Preferably, the composition further comprises a pharmaceutically, food or nutraceutical acceptable carrier.
Preferably, the composition is in a solid or liquid form.
Preferably, the composition is in the form of oral preparation or injection preparation.
Preferably, the composition is a food composition, and the food composition is a beverage or yogurt.
Preferably, the composition is in the form of emulsion, tablet, capsule, oral liquid, granule, powder or syrup.
Preferably, the beverage is a healthy balanced nutritional beverage or a sports beverage.
Preferably, the yoghurt is yoghurt, fermented milk, flavored yoghurt or flavored fermented milk.
Preferably, the ratio (mg: IU) of the pueraria flower extract to the vitamin D is (1-40): (80-320), preferably (5-20): (100- & ltSUB & gt 300) ], more preferably (5-15): (150-250), more preferably (8-12): (180-220).
Preferably, the weight ratio of the pueraria flower extract to the calcium carbonate is (1-40): (500-1200), preferably (5-20): (600- > 1000), more preferably (5-15): (700-900), more preferably (8-12): (750-850).
Preferably, the content of the pueraria flower extract is 1-99 wt%, preferably 10-90 wt%, more preferably 20-80 wt%, based on the total weight of the composition.
Preferably, the vitamin D is present in an amount of 1 to 99 wt%, preferably 10 to 90 wt%, more preferably 20 to 80 wt%, based on the total weight of the composition.
In a third aspect, the present invention provides a use of the composition of the third aspect of the present invention for preparing a preparation for promoting oral absorption of calcium.
Preferably, the calcium comprises calcium from calcium carbonate.
Preferably, the formulation is an oral formulation.
In a fifth aspect, the present invention provides a calcium-containing composition comprising a pueraria flower extract according to the first aspect of the present invention, vitamin D, and calcium carbonate.
Preferably, the calcium-containing composition is a beverage or a yoghurt.
Preferably, the dosage form of the calcium-containing composition is an oral formulation.
Preferably, said vitamin D comprises vitamin D3.
Adding 55-65% (v/v) ethanol water solution 6-10 times the amount of flos Puerariae Lobatae, heating and reflux extracting for 4-8 hr, filtering, concentrating the filtrate under reduced pressure to obtain concentrated solution, adding petroleum ether into the concentrated solution for extraction, distilling the obtained petroleum ether organic phase layer under reduced pressure to remove solvent, and drying to obtain flos Puerariae Lobatae extract.
Preferably, the 55-65% (v/v) ethanol aqueous solution is 60% (v/v) ethanol aqueous solution.
Preferably, the ratio (mg: IU) of the pueraria flower extract to the vitamin D is (1-40): (80-320), preferably (5-20): (100- & ltSUB & gt 300) ], more preferably (5-15): (150-250), more preferably (8-12): (180-220).
Preferably, the weight ratio of the pueraria flower extract to the calcium carbonate is (1-40): (500-1200), preferably (5-20): (600- > 1000), more preferably (5-15): (700-900), more preferably (8-12): (750-850).
In a sixth aspect the present invention provides the use of a calcium-containing composition according to the fifth aspect of the invention for the preparation of a medicament for (i) the prevention and/or treatment of calcium deficiency; and/or (ii) an agent for the prevention and/or treatment of a disease associated with calcium deficiency.
In a preferred embodiment, said calcium deficiency comprises a blood calcium deficiency.
In a preferred embodiment, the calcium deficiency comprises blood calcium deficiency.
It is to be understood that within the scope of the present invention, the above-described features of the present invention and those specifically described below (e.g., in the examples) may be combined with each other to form new or preferred embodiments.
Detailed Description
The invention develops a pueraria flower extract which can effectively promote the oral absorption of calcium, improve the level of blood calcium and treat calcium deficiency. The experimental research of the invention also shows that the pueraria flower extract and the vitamin D can synergistically promote the oral absorption of calcium, so that the pueraria flower extract and the vitamin D can be used together to further promote the oral absorption of calcium and treat calcium deficiency.
Term(s) for
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
As used herein, the terms "comprising," "including," and "containing" are used interchangeably and include not only open-ended definitions, but also semi-closed and closed-ended definitions. In other words, the term includes "consisting of … …", "consisting essentially of … …".
As used herein, the "flower of kudzu" is a flower of the leguminous plant kudzu vine lobata (Willd.) Ohwi, kudzu p.
As used herein, the term "IU" is an international unit.
As used herein, the terms "60% (v/v) ethanol" or "60% (v/v) aqueous ethanol" are used interchangeably to refer to an aqueous ethanol solution having a volume fraction of ethanol of 60%, e.g., 60% ethanol is formulated from 60ml absolute ethanol +40ml water.
Vitamin D3(Vitamin D3) is one of Vitamin D.
Flos puerariae lobatae extract and its use
The invention provides a pueraria flower extract, which is preferably a dry extract or a dry extract.
Preferably, the pueraria flower extract is prepared by the following method:
(1) extracting flos Puerariae Lobatae with alcohol solvent to obtain alcohol extractive solution;
(2) concentrating the alcohol extract, adding an organic solvent for extraction, and carrying out vacuum distillation on the organic phase to remove the solvent to obtain the pueraria flower extract, wherein the organic solvent comprises one or more of petroleum ether, chloroform and ethyl acetate.
Preferably, in the step (1), the alcohol solvent is a C1-C4 lower alcohol or a C1-C4 lower alcohol aqueous solution.
Preferably, the C1-C4 lower alcohol comprises one or more of methanol, ethanol, propanol and butanol.
Preferably, the volume fraction of the C1-C4 lower alcohol in the C1-C4 lower alcohol aqueous solution is 40-70%, preferably 50-60%, more preferably 55-65%.
Preferably, in the step (2), the organic solvent is petroleum ether.
Preferably, the pueraria flower extract is prepared by the following method:
adding 55-65% (v/v) ethanol water solution 6-10 times the amount of flos Puerariae Lobatae, heating and reflux extracting for 4-8 hr, filtering, concentrating the filtrate under reduced pressure to obtain concentrated solution, adding petroleum ether into the concentrated solution for extraction, distilling the obtained petroleum ether organic phase layer under reduced pressure to remove solvent, and drying to obtain flos Puerariae Lobatae extract.
The invention also provides application of the pueraria flower extract in preparing a composition for promoting oral absorption of calcium.
Preferably, the calcium comprises calcium from calcium carbonate.
Composition and use thereof
The invention also provides a composition which can be a pharmaceutical composition, a food composition or a health-care product composition.
The composition also comprises a carrier acceptable in pharmacy, food or health care products.
As used herein, the term "pharmaceutically, comestibly or nutraceutically acceptable carrier" refers to: one or more compatible solid, semi-solid, liquid or gel fillers suitable for human or animal use and of sufficient purity and sufficiently low toxicity.
It is to be understood that, in the present invention, the carrier is not particularly limited and may be selected from materials commonly used in the art, or prepared by a conventional method, or commercially available. Examples of the pharmaceutically acceptable carrier moiety are cellulose and its derivatives (e.g., methylcellulose, ethylcellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, etc.), gelatin, talc, solid lubricants (e.g., stearic acid, magnesium stearate), calcium sulfate, vegetable oils (e.g., soybean oil, sesame oil, etc.), polyols (e.g., propylene glycol, glycerin, sorbitol, etc.), emulsifiers (e.g., tween), wetting agents (e.g., sodium laurylsulfate), buffers, chelating agents, thickeners, pH adjusters, transdermal enhancers, colorants, flavors, stabilizers, antioxidants, preservatives, bacteriostats, pyrogen-free water, etc.
In the present invention, the dosage form of the pharmaceutical composition includes, but is not limited to, oral preparations or injectable preparations.
Typically, the composition is in the form of emulsion, tablet, capsule, oral liquid, granule, powder or syrup.
Typically, the composition, preparation or calcium-containing composition of the invention is a beverage or a yoghurt.
Typically, the composition, preparation or calcium-containing composition according to the invention is a beverage. The pueraria flower extract can be prepared into a beverage, and the promotion of the oral absorption of calcium can be simply and conveniently realized through the oral beverage, so that the calcium supplement of the public can be conveniently realized.
In the present invention, the beverage is not particularly limited, and may be, for example, a solid beverage, a liquid beverage, a semisolid beverage, or the like.
In a preferred embodiment of the invention, the beverage further comprises a calcium supplement, such as calcium gluconate.
Typically, the beverage of the present invention is a healthy balanced nutritional beverage or a sports beverage.
Typically, the beverage of the present invention may be a healthy and balanced nutritional beverage component, for example, the beverage of the present invention may contain nutritional components such as amino acids, monosaccharides (e.g., glucose, fructose), fatty acids, vitamins, electrolytes (e.g., calcium ions, sodium ions, potassium ions), egg yolk lecithin, and sodium casein, and the nutritional components can supplement calcium while achieving other nutritional components, and particularly, the beverage is suitable for calcium supplementation and balanced nutritional supplementation for children, the elderly and other people.
Typically, the beverage of the present invention is also a sports beverage. The sports drink is prepared according to the characteristics of physiological consumption during sports, can purposefully supplement nutrition lost during sports, and plays the roles of maintaining and improving the sports ability and accelerating the fatigue elimination after sports. The sports beverage according to the present invention may comprise one or more ingredients selected from the group consisting of: sucrose, glucose, vitamins (e.g., vitamin B12, vitamin C), electrolytes (e.g., calcium, sodium, potassium), citric acid, and the like. When the sports beverage is taken before or after sports, the pueraria flower extract can promote the oral absorption of calcium, thereby supplementing the calcium loss or calcium deficiency caused by sports in time.
Typically, the composition, preparation or calcium-containing composition according to the invention is a yoghurt. The pueraria flower extract can be prepared into yoghourt, and the promotion of the oral absorption of calcium can be simply and conveniently realized through the oral yoghourt, so that the calcium supplement of the public can be conveniently realized.
The yoghourt is a sour and sweet milk beverage, and is a milk product which is prepared by taking milk as a raw material, adding beneficial bacteria (leavening agent) into the milk after pasteurization, fermenting, and then cooling and filling. In the market, most of the yoghourt products are set type, stirring type and fruit taste type added with various auxiliary materials such as fruit juice, jam and the like.
The pueraria flower extract can promote the absorption of calcium, so that the pueraria flower extract can be prepared into yoghourt to better promote the absorption of calcium in a yoghourt product.
Yoghurts of the invention include, but are not limited to, yogurt, fermented milk, flavored yogurt, or flavored fermented milk.
The yogurt is prepared from raw cow (sheep) milk or milk powder as raw material by sterilizing, inoculating Streptococcus thermophilus and Lactobacillus bulgaricus (Lactobacillus delbrueckii subsp. bulgaricus), and fermenting.
The fermented milk is prepared from raw cow (sheep) milk or milk powder as raw material by sterilizing and fermenting to obtain product with reduced pH value.
The yoghourt of the flavored yogurt is added with other components such as food additives, fruits, vegetables or grains and the like besides milk/milk powder. The milk/milk powder content is more than 80 percent, and the protein content is more than or equal to 2.3 percent.
Besides milk/milk powder, other components are added after inoculation and fermentation of the flavored fermented milk.
Typically, the yoghurt of the invention is a flavoured yoghurt. The taste of the flavored yogurt is better suitable for children and old people who supplement calcium, so that the flavored yogurt prepared from the pueraria flower extract can better and effectively promote children and old people to improve the oral supplement of calcium by orally taking the yogurt, and the calcium deficiency is treated.
In a preferred embodiment of the present invention, the composition comprises the pueraria flower extract and vitamin D.
Preferably, said vitamin D comprises vitamin D3.
Preferably, the composition further comprises L-arabinose.
Preferably, the composition further comprises calcium carbonate.
Preferably, the ratio (mg: IU) of the pueraria flower extract to the vitamin D is (1-40): (80-320), preferably (5-20): (100- & ltSUB & gt 300) ], more preferably (5-15): (150-250), more preferably (8-12): (180-220).
Preferably, the weight ratio of the pueraria flower extract to the calcium carbonate is (1-40): (500-1200), preferably (5-20): (600- > 1000), more preferably (5-15): (700-900), more preferably (8-12): (750-850).
The invention also provides an application of the composition provided by the invention in preparing a preparation for promoting the oral absorption of calcium.
Preferably, the calcium comprises calcium from calcium carbonate.
Preferably, the formulation is an oral formulation.
In a preferred embodiment of the present invention, the present invention further provides a calcium-containing composition, wherein the calcium-containing composition comprises the pueraria flower extract of the present invention, vitamin D and calcium carbonate.
Preferably, the calcium-containing composition is a beverage or a yoghurt.
Preferably, the dosage form of the calcium-containing composition is an oral formulation.
Preferably, said vitamin D comprises vitamin D3.
Preferably, the ratio (mg: IU) of the pueraria flower extract to the vitamin D is (1-40): (80-320), preferably (5-20): (100- & ltSUB & gt 300) ], more preferably (5-15): (150-250), more preferably (8-12): (180-220).
Preferably, the weight ratio of the pueraria flower extract to the calcium carbonate is (1-40): (500-1200), preferably (5-20): (600- > 1000), more preferably (5-15): (700-900), more preferably (8-12): (750-850).
The present invention also provides the use of a calcium-containing composition according to the invention for the preparation of a medicament for (i) the prevention and/or treatment of calcium deficiency; and/or (ii) an agent for the prevention and/or treatment of a disease associated with calcium deficiency.
In a preferred embodiment, said calcium deficiency comprises a blood calcium deficiency.
In a preferred embodiment, the calcium deficiency comprises blood calcium deficiency.
The main technical effects of the invention comprise:
1. the pueraria flower extract can obviously improve the oral absorption of calcium and improve the treatment of calcium deficiency by oral calcium.
2. The pueraria flower extract and the vitamin D can synergistically promote the oral absorption of calcium, so that the pueraria flower extract and the vitamin D can be used together to further promote the oral absorption of calcium and treat calcium deficiency.
3. The pueraria flower extract can be orally taken in a beverage or yoghourt mode to promote calcium absorption, so that the taking compliance of children and old people can be improved.
The invention will be further illustrated with reference to the following specific examples. It should be understood that the following specific examples are provided to illustrate the detailed embodiments and specific procedures, but the scope of the present invention is not limited to these examples.
Example 1
(1) Preparing a pueraria flower extract, wherein the pueraria flower extract is prepared by the following method:
(a) weighing 50g of pueraria flower, crushing, adding 400ml of 60% (v/v) ethanol water solution, heating, refluxing, extracting for 6 hours, filtering, and concentrating the filtrate under reduced pressure to 100ml to obtain a concentrated solution;
(b) extracting the concentrated solution obtained in the step (a) with 300mL of petroleum ether each time, extracting twice in total, combining petroleum ether layers obtained by two times of separation (namely petroleum ether extract), removing the petroleum ether by reduced pressure distillation, and drying to obtain the pueraria flower extract.
(2) The calcium absorption-promoting effect of the pueraria flower extract prepared in this example was examined
Laboratory animal
Experimental animals and their rearing: KM mice, pathogen Free (SPF) grade, male, 6 weeks old, adapted for 1 week prior to experimental use.
Experimental methods
KM mice were fasted at 21:00 nights without water prohibition before the experiment, and were randomly divided into experiment group 1, experiment group 2, experiment group 3, experiment group 4 and control group at 8:00 morning the next day, 8 mice in each group, wherein mice of experiment group 1 were gavaged with calcium carbonate aqueous suspension (calcium carbonate dosage is 0.8g/kg body weight), mice of experiment group 2 were gavaged with calcium carbonate and flos Puerariae Lobatae extract aqueous suspension (calcium carbonate dosage is 0.8g/kg body weight and flos Puerariae Lobatae extract dosage is 10mg/kg body weight), mice of experiment group 3 were gavaged with calcium carbonate and vitamin D3 aqueous suspension (calcium carbonate dosage is 0.8g/kg body weight and vitamin D3 dosage is 200IU/kg body weight), mice of experiment group 4 were gavaged with calcium carbonate, flos Puerariae Lobatae extract and vitamin D3 aqueous suspension (calcium carbonate dosage is 0.8g/kg body weight, water is not prohibited, and mice of experiment group 4 are controlled by 8:00 morning the following day, The administration dose of the pueraria lobata flower extract was 10mg/kg body weight and the administration dose of the vitamin D3 was 200IU/kg body weight), and the mice in the control group were gavaged with physiological saline. The mice of each group are subjected to blood sampling before (0h) and 1h after the intragastric administration, serum is obtained through centrifugation, the calcium ion concentration in the serum is measured by adopting a full-automatic biochemical analyzer, and the mice of each group are fasted without water supply after the administration.
Statistical analysis:
metering using
Figure BDA0002837145810000101
Analyzing data by SPSS software, comparing two by t test, and taking p<0.05 is statistically significant.
(3) Results of the experiment
Ca in serum before and after administration to mice of each group2+The contents are shown in table 1:
TABLE 1 Ca in serum before and after administration to mice of each group2+The content of (c) (mmol/L,
Figure BDA0002837145810000102
n＝8)
Figure BDA0002837145810000103
p <0.05 and p <0.01, compared to 1h after administration to the control group.
As can be seen from table 1, the pueraria lobata extract can effectively promote the oral absorption of calcium and increase the blood calcium level. From the data of examples 1-4, it can be seen that the pueraria lobata flower extract and vitamin D3 synergistically promote the intestinal absorption of calcium, thereby significantly increasing the blood calcium level and treating calcium deficiency and related diseases such as osteoporosis.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims (6)

1. A composition for promoting calcium absorption by oral administration, wherein said composition is prepared from pueraria flower extract and vitamin D3;
the ratio mg: IU of the pueraria flower extract to the vitamin D3 is 5-20: 100-300;
the pueraria flower extract is prepared by the following method:
(1) extracting flos Puerariae Lobatae with 55-65% ethanol solvent to obtain ethanol extractive solution;
(2) concentrating the alcohol extract, adding an organic solvent for extraction, and carrying out vacuum distillation on the organic phase to remove the solvent to obtain the pueraria flower extract, wherein the organic solvent is petroleum ether.
2. The composition of claim 1, wherein the composition is a food composition and the food composition is a beverage or yogurt.
3. The composition of claim 2, wherein the beverage is a healthy balanced nutritional beverage or a sports drink.
4. The composition of claim 1, wherein the pueraria lobata extract is prepared by the following method:
adding 55-65% ethanol water 6-10 times the volume of flos Puerariae Lobatae, heating and reflux extracting for 4-8 hr, filtering, concentrating the filtrate under reduced pressure to obtain concentrated solution, adding petroleum ether into the concentrated solution for extraction, distilling the obtained petroleum ether organic phase layer under reduced pressure to remove solvent, and drying to obtain flos Puerariae Lobatae extract.
5. The composition of claim 1, wherein the ratio of the pueraria lobata flower extract to the vitamin D3 mg IU is 8-12: 180-220.
6. Use of a composition according to claim 1 for the preparation of a formulation for promoting oral absorption of calcium.
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