CN114591405A - 猫杯状病毒疫苗 - Google Patents

猫杯状病毒疫苗 Download PDF

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CN114591405A
CN114591405A CN202210237354.2A CN202210237354A CN114591405A CN 114591405 A CN114591405 A CN 114591405A CN 202210237354 A CN202210237354 A CN 202210237354A CN 114591405 A CN114591405 A CN 114591405A
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E.舒胡
A.S.鲍格
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Abstract

本发明涉及猫杯状病毒疫苗。具体涉及新的猫衣壳蛋白，包含该衣壳蛋白的活减毒猫杯状病毒，包含该衣壳蛋白的活重组载体病毒和活减毒杂合猫杯状病毒，包含此类活减毒猫杯状病毒的疫苗，活重组载体病毒和活减毒杂合猫杯状病毒，以及用于制备此类病毒的方法。

Description

猫杯状病毒疫苗
本申请是申请日为2016年12月22日的中国专利申请201680076354.1 “猫杯状病毒疫苗”的分案申请。
技术领域
本发明涉及新的猫杯状病毒衣壳蛋白，包含该衣壳蛋白的活减毒猫杯状病毒，包含该衣壳蛋白的活重组载体病毒和活减毒杂合猫杯状病毒，包含此类活减毒猫杯状病毒的疫苗，活重组载体病毒和活减毒杂合猫杯状病毒，以及用于制备此类病毒的方法。
背景技术
猫杯状病毒(FCV)是属于杯状病毒科中的水疱疹病毒属的单链正义RNA病毒。该病毒是高度感染性的，并且引起猫的上呼吸道疾病(URD)和口腔溃疡。该病毒还与慢性牙龈炎和口腔炎相关。更近来，出现了通常称为VS-FCV的毒性全身性猫杯状病毒，其引起高死亡率、水肿和溃疡性皮肤损害和黄疸。
FCV的传播主要通过与鼻腔或口腔分泌物接触(Scherk, M. A.等人, Journal of Feline Medicine and Surgery (2013) 15, 补充文件)。
杯状病毒科的基因组和基因组组织是本领域中众所周知的。总体概述尤其由Clarke, J.等人(Inf.Diseases 181 (Suppl. 2):S309-316 (2000))公开。猫杯状病毒的第一个完整基因组序列已经于1992年公开(Carter, M. J.等人, Virology 190:443-448(1992))，并且在后来的年代中，多得多猫杯状病毒的完整基因组序列已经公开(尤其通过Oka, T.等人, GenomeA, May/June 2013, vol. 1, issue 3, e00349-13,Genomea.asm.org)，并且可尤其通过Genbank得到。
FCV的基因组仅包含三个开放阅读框；ORF 1、2和3。ORF1编码大非结构性多蛋白。ORF3，短3’-末端ORF，编码被认为参与基因组RNA的衣壳化的小蛋白。
ORF 2是编码FCV衣壳蛋白的开放阅读框。已知衣壳蛋白是触发宿主中的保护性免疫应答的蛋白。因此，衣壳蛋白是用于开发用于针对FCV感染保护猫的疫苗的靶蛋白。
FCV毒株在全世界仅包含一种血清型，并且主要包含一种血清群。然而，在毒株之间存在相当大的遗传性变异，且因此存在抗原性变异。这种高水平的抗原性变异使得难以获得在猫中针对FCV的广泛保护：尽管用同源毒株接种非常有效，但一种毒株针对另一种毒株的交叉保护水平是相当可变的。(Coyne C.P.等人, J. Virol. 86: 11356-11367(2012))。
目前，修饰的活疫苗和灭活疫苗是可得的，并且它们通常全身施用。最初，疫苗曾经是单一疫苗，其主要基于毒株FCV F9或FCV 255。然而，它们都受困于上面鉴定的问题：缺乏广泛的交叉保护。
目前，至少部分地通过施用包含两种不同的FCV毒株(诸如FCV 431和FCV G1)的二价疫苗来在一定程度上绕开该问题。
此类(灭活的)二价疫苗目前在美国和欧洲均商业可得。(Poulet H, 等人, Vaccine 26: 3647–3654 (2008), Chengjin Huang等人, Journal of Feline Medicineand Surgery February 12: 129-137 (2010))。
McCabe V. J.等人开发了活减毒和灭活疫苗的替代方案，其构建了一种活减毒重组载体病毒(LARCV)，在该情况下是粘液瘤病毒，其表达FCV衣壳蛋白，并且成功地将该重组粘液瘤病毒作为LARCV疫苗施用于猫。
此类基于重组粘液瘤-载体的疫苗具有这样的优点：载体是减毒的，不在猫中复制并且仅携带编码FCV衣壳蛋白的FCV ORF。因此，接种后没有FCV或载体病毒脱落至环境中。
表达FCV衣壳蛋白的活减毒重组载体病毒的另一个实例是如由Yokoyama, N.等人(J. Vet. Med. Sci. 60:717-723 (1998))所述的猫疱疹病毒载体。该载体还用于针对FCV接种猫。
然而，还没有开发既安全又显示广泛水平的交叉保护的活减毒FCV疫苗或基于重组载体的疫苗。
发明内容
本发明的一个目标是提供安全且仍显示广泛水平的交叉保护的FCV疫苗。
令人惊讶地发现，存在迄今未知的FCV毒株，其中衣壳蛋白显示针对许多FCV野外毒株的显著广谱的交叉保护。该毒株的代表的衣壳蛋白的实例描绘于SEQ ID NO:34中。其衣壳蛋白序列显示于SEQ ID NO:34中的该新型FCV毒株的代表进一步被称为FCV毒株KalemCrouch。
表1显示针对本发明的新型FCV毒株产生的抗血清与31种其他FCV毒株的交叉中和特性。显示病毒滴度的log10降低。>1.5 log10的滴度降低被认为是显著的。
如下表所示，针对新型FCV毒株Kalem Crouch产生的抗血清令人惊讶地中和了测试的31种FCV毒株中的26种。与常用的F9毒株进行比较。如表1中可见，看到针对F9产生的抗血清仅显示对于测试的22种FCV毒株中的3种的滴度的显著降低。
已将该新FCV毒株的衣壳蛋白的氨基酸序列与24种其他FCV衣壳蛋白的已知氨基酸序列进行比较，并且可以得出结论，该序列与已知的FCV衣壳蛋白相当显著不同。如从图5可见，新毒株的衣壳蛋白和已知的FCV毒株的衣壳蛋白之间的总体序列同一性为约87％。此外，新衣壳蛋白的几个独特氨基酸被鉴定为K89、M90、M100、I317、L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518、I524、S545、S634、F635、P636。此外，氨基酸447-452的序列KLEYEN和氨基酸489-493的GVISD也是独特的。
应该理解的是，对于SEQ ID NO:34的氨基酸序列和编码蛋白的DNA，在该毒株的各个代表之间可以存在较小的天然变异。首先，在核苷酸水平上，存在所谓的“第二和第三碱基的摆动”，解释可以存在这样的核苷酸变化，其在其编码的氨基酸序列中仍然未被注意到：例如，三联体TTA、TTG、TCA、TCT、TCG和TCC都编码亮氨酸。此外，在FCV的代表之间可以存在核苷酸水平上的轻微变异，这可以导致氨基酸序列的微小变异。这些变异可以通过总体序列中的(一个或多个)氨基酸差异或通过所述序列中的(一个或多个)氨基酸的缺失、取代、插入、倒置或添加来反映。基本上不改变生物学和免疫学活性的氨基酸取代已经例如由Neurath等人于"The Proteins" Academic Press New York (1979)中描述。相关氨基酸之间的氨基酸替代或进化中已频繁发生的替代尤其是Ser/Ala、Ser/Gly、Asp/Gly、Asp/Asn、Ile/Val (参见Dayhof, M.D., Atlas of protein sequence and structure, Nat.Biomed. Res. Found., Washington D.C., 1978, vol. 5, suppl. 3)。其他氨基酸取代包括Asp/Glu、Thr/Ser、Ala/Gly、Ala/Thr、Ser/Asn、Ala/Val、Thr/Phe、Ala/Pro、Lys/Arg、Leu/Ile、Leu/Val和Ala/Glu。基于该信息，Lipman和Pearson开发了一种用于快速和灵敏的蛋白比较(Science, 227, 1435-1441, 1985)和确定相同蛋白之间的功能相似性的方法。本发明的示例性实施方案的此类氨基酸取代以及具有缺失和/或插入的变异在本发明的范围内，只要所得蛋白保留其免疫反应性。这解释了为什么根据本发明的FCV衣壳蛋白，当从不同的野外分离株分离时，可以具有约90％的同一性水平，同时仍然代表具有相当的免疫交叉反应性的蛋白。
因此，本发明的第一个实施方案涉及与如SEQ ID NO:34中给出的氨基酸序列具有至少90％的序列同一性的猫杯状病毒衣壳蛋白。
任选地，所述衣壳蛋白与如SEQ ID NO:34中给出的氨基酸序列具有至少91%、92%、93%、94%、95%、96%、97%、98%、99%或100％的序列同一性，按优先顺序递增。
本发明和/或其实施方案的另一个实施方案涉及包含与如SEQ ID NO:34中给出的氨基酸序列具有至少90％的序列同一性的衣壳蛋白的活减毒FCV。
本发明和/或其实施方案的另一个实施方案涉及包含根据本发明和/或其任何实施方案的衣壳蛋白的活减毒FCV。
任选地，所述FCV具有衣壳蛋白，所述衣壳蛋白与如SEQ ID NO:34中给出的氨基酸序列具有至少91%、92%、93%、94%、95%、96%、97%、98%、99%或100％的序列同一性，按优先顺序递增。
此外，本发明涉及包含以下氨基酸中的至少一个的猫杯状病毒衣壳蛋白：K89、M90、M100、I317、L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518、I524、S545、S634、F635、P636。合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸中的至少一个或多个：K89、M90、M100、I317、L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518、I524、S545、S634、F635、P636。合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸中的至少一个、两个、三个、四个、五个或更多个：K89、M90、M100、I317、L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518、I524、S545、S634、F635、P636。
此外，本发明涉及包含以下氨基酸中的至少一个的猫杯状病毒衣壳蛋白：I317、L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518、I524或S545。合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸中的至少一个或多个：I317、L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518、I524或S545。合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸中的至少一个、两个、三个、四个、五个或更多个：I317、L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518、I524或S545。
此外，本发明涉及包含以下氨基酸中的至少一个的猫杯状病毒衣壳蛋白：L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518或I524。合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸中的至少一个或多个：L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518或I524。合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸中的至少一个、两个、三个、四个、五个或更多个：L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518或I524。
合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸：K89、M90和M100。
合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸：I317。
合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸：L390、A391、V392、Q396、S397、K398和N404。
合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸：T426、T431、S438、S437、D440、E445、K447、L448、E451和N452。
合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸：G484、G489和I491。
合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸：N516、S517、E518和I524。
合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸：S545。
合适地，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸：S634、F635和P636。
明确设想根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含上述氨基酸组的组合。例如，根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白包含以下氨基酸：L390、A391、V392、Q396、S397、K398、N404和I317。另一个实例涉及根据本发明和/或其实施方案的猫杯状病毒衣壳蛋白，其包含以下氨基酸：N516、S517、E518、I524和S545。
此外，本发明涉及猫杯状病毒衣壳蛋白，其中氨基酸447-452是KLEYEN且/或其中氨基酸489-493是GVISD。合适地，本发明涉及猫杯状病毒衣壳蛋白，其中氨基酸447-452是KLEYEN且氨基酸489-493是GVISD。合适地，其中氨基酸447-452是KLEYEN且/或其中氨基酸489-493是GVISD的猫杯状病毒衣壳蛋白也包含以下氨基酸中的至少一个：K89、M90、M100、I317、L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、G484、N516、S517、E518、I524、S545、S634、F635或P636。
本发明和/或其实施方案的另一个实施方案涉及包含衣壳蛋白的活减毒FCV，所述衣壳蛋白与如SEQ ID NO:34中给出的氨基酸序列具有至少90％的序列同一性且具有以下氨基酸中的至少一个：K89、M90、M100、I317、L390、A391、V392、Q396、S397、K398、N404、T426、T431、S438、S437、D440、E445、K447、L448、E451、N452、G484、G489、I491、N516、S517、E518、I524、S545、S634、F635、P636。
根据本发明的衣壳蛋白或编码衣壳蛋白的区域诸如ORF2或其片段可以几种方式用于制备用于针对FCV保护猫的疫苗。
包含编码根据本发明和/或其实施方案的衣壳蛋白的区域的DNA片段可以例如用于制备包含根据本发明和/或其实施方案的衣壳蛋白的非-FCV重组载体病毒。它也可以用于制备杂合FCV，如下所述。
因此，本发明的第三个实施方案涉及DNA片段，其特征在于其包含编码根据本发明和/或其实施方案的衣壳蛋白的区域。
在其中使用重组载体作为包含编码根据本发明和/或其实施方案的衣壳蛋白的区域的DNA片段的载体的情况下，衣壳蛋白的表达通常通过将包含编码根据本发明和/或其实施方案的衣壳蛋白的区域的DNA片段置于合适的异源启动子的控制下来获得。
合适的启动子是能够驱动位于宿主细胞(在该情况下，真核的，更具体地猫细胞)中启动子下游的编码区的转录的启动子。用于表达FCV衣壳蛋白的大量合适的启动子是本领域中已知的，其被公认用于它们的有效水平的表达。此类启动子包括经典启动子，诸如(人)巨细胞病毒立即早期启动子(Sun-Young Lee 等人, Journal of BiomedicalScience 6: 8-17 (1999), Seed, B. 等人, Nature 329, 840-842, 1987; Fynan, E.F.等人, PNAS 90, 11478-11482,1993; Ulmer, J.B. 等人, Science 259, 1745-1748,1993)，人巨细胞病毒增强子-启动子(Donofrio G., 等人, Clinical and VaccineImmunology 13: 1246-1254, (2006))，小鼠巨细胞病毒立即早期(MCMVie1)启动子，小鼠巨细胞病毒早期(MCMVe1)启动子，SV40立即早期启动子(Sprague J. 等人, J. Virology45, 773 ,1983)，SV-40启动子(Berman, P.W. 等人, Science, 222, 524-527, 1983)，金属硫蛋白启动子(Brinster, R.L. 等人, Nature 296, 39-42, 1982)，热休克启动子(Voellmy 等人, Proc. Natl. Acad. Sci. USA, 82, 4949-53, 1985)，Ad2的主要晚期启动子，β-肌动蛋白启动子(Tang 等人, Nature 356, 152-154, 1992)和CAG启动子(Miyazaki, J; Takaki, S; Araki, K; Tashiro, F; Tominaga, A; Takatsu, K;Yamamura, K., Gene 79 (2): 269–277 (1989)和Niwa, H; Yamamura, K; Miyazaki,J., Gene 108 (2): 193–199 (1991))。
合适地，将编码衣壳蛋白的区域置于合适启动子的控制下。
包含编码根据本发明和/或其实施方案的衣壳蛋白的区域的DNA片段可以例如是质粒。该质粒可以是环状或线性形式。
鉴于根据本发明和/或其实施方案的衣壳蛋白提供的广泛保护，在活重组载体病毒中使用编码根据本发明和/或其实施方案的衣壳蛋白的区域是有吸引力的。
此类活减毒重组载体病毒(LARCV)是能够感染宿主动物(在该情况下，猫物种)并携带在合适启动子的控制下的外源基因(在该情况下，编码根据本发明和/或其实施方案的衣壳蛋白的区域)的重组病毒。
LARCV及其用途已经尤其由Souza, A.P.D.等人于Braz. J. Med. Biol. Res,38:509-522 (2005)中综述。此类活重组载体病毒的实例是：痘病毒(尤其痘苗病毒)、腺病毒、疱疹病毒、粘液瘤病毒和更近期甲病毒。
因此，本发明的第四个实施方案涉及包含在启动子的控制下的编码根据本发明的衣壳蛋白的区域的活减毒重组载体病毒(LARCV)。
McCabe等人提供了此类减毒活重组载体病毒的实例，McCabe等人描述了粘液瘤病毒作为FCV毒株F9的衣壳蛋白的LARCV的用途(参见上文)。
表达FCV衣壳蛋白的活减毒重组载体病毒的另一个实例是表达FCV衣壳蛋白的猫疱疹病毒载体(诸如由Yokoyama, N.等人描述)(参见上文)。
合适地，活减毒重组载体病毒是粘液瘤病毒或猫疱疹病毒。
本发明和/或其实施方案的另一个实施方案涉及根据本发明和/或其实施方案的活减毒重组载体病毒，其用于针对FCV感染保护猫。
衣壳蛋白及其编码区也可以允许用于针对FCV保护猫的另一种方法。该方法涉及杂合FCV。
本领域已知存在用于针对FCV感染保护猫的活减毒疫苗。这种活减毒FCV的一个实例是FCV毒株F9，已知其在全身施用时提供安全的活疫苗。然而，如上所提及，一般FCV毒株和还有F9毒株都不能针对其他FCV毒株感染猫提供广泛的交叉保护。
现在令人惊讶地发现，包含编码根据本发明和/或其实施方案的衣壳蛋白的区域和来自减毒FCV的ORF1的杂合FCV毒株提供了高水平的安全性和广泛的交叉保护。
另一个实施方案涉及活减毒杂合FCV，其特征在于所述FCV包含编码根据本发明和/或其实施方案的衣壳蛋白的区域，并且包含编码来自减毒FCV的开放阅读框1(ORF1)的减毒的区域。
合适地，本发明和/或其实施方案的活减毒杂合FCV包含编码根据本发明和/或其实施方案的衣壳蛋白的开放阅读框2(ORF2)，并且包含来自减毒FCV的开放阅读框1(ORF1)。
用于构建杂合杯状病毒的方法是本领域众所周知的。对于猫杯状病毒，Neill等人描述了不同FCV毒株之间的衣壳蛋白结构域交换(Neill, D. J.等人, J. Virol 74:1079-1084 (2000))。Thumfart J. O.和Meyers G. (J. Virol. 76:6398-6407 (2002))已经在2002年描述了用于在转染cRNA或cDNA构建体后从细胞中回收重组病毒的其他方法。Aubry,F.等人近期已经描述了使用亚基因组扩增子生成单链正义RNA病毒的甚至更快的方法(J.Gen. Virol 95:2462-2467 (2014))。
减毒病毒可以例如通过如下获得：使根据本发明和/或其实施方案的病毒在诱变剂存在的情况下生长，随后选择显示子代水平和/或复制速度降低的病毒。许多此类试剂是本领域已知的。
另一种频繁使用的减毒方法是系列体外传代。在该过程期间，病毒变得适应于用于系列传代的细胞系。作为结果，它们当随后作为疫苗再次施用于天然宿主时表现出减毒。
获得减毒病毒的又另一种方式是使它们在偏离其天然栖息地温度的温度下生长。温度敏感突变体(Ts-突变体)的选择方法是本领域中众所周知的。此类方法包括使病毒在诱变剂存在的情况下生长，随后在亚最佳温度和最佳温度下生长，在细胞层上滴定子代病毒，以及视觉选择在最佳温度下较慢生长的那些噬斑。此类小噬斑包含缓慢生长且因此期望的减毒活病毒。
用于生成减毒单链正义RNA病毒的更直接和可预测的方法例如由Weeks, S. A.等人于J. of Biol.Chem., 287:31618-31622 (2014)中描述。
任选地，技术人员将使用编码来自开放阅读框1(ORF1)的减毒的区域或来自已有的减毒FCV毒株的ORF1。这种活减毒病毒的众所周知的实例是FCV毒株F9。
Kalunda等人AJVR (1975) 36:353-356描述了作为疫苗的毒株FCV-F9的特性。还参见Bittle,等人, Ibid. (1976) 37:275-278。
合适地，本发明和/或其实施方案涉及根据本发明的活减毒杂合FCV，其包含编码来自开放阅读框1(ORF1)的减毒的区域或获得自FCV毒株F9的开放阅读框1(ORF1)。
在实施例部分中，详细描述了用于制备如上所述的根据本发明和/或其实施方案的杂合FCV的方法。
本发明的再另一个实施方案涉及根据本发明和/或其实施方案的活减毒杂合FCV，其用于针对FCV感染保护猫。
适合于培养活重组载体病毒的哺乳动物细胞是本领域已知的。此类细胞是支持已知LARCV(诸如痘病毒、腺病毒、疱疹病毒、粘液瘤病毒和更近期甲病毒)的生长的细胞。
表达FCV衣壳蛋白的减毒重组的基于粘液瘤的载体病毒可以例如在RK13细胞上生长。表达FCV衣壳的减毒重组的基于猫疱疹病毒的载体病毒可以例如在Crandell-Rees猫肾(CRFK)细胞上生长。
同样地，适合于培养活减毒FCV和活减毒杂合FCV的细胞是本领域中已知的。用于使FCV生长的最常见的细胞是CRFK细胞。
因此，本发明的再另一个实施方案涉及包含根据本发明和/或其实施方案的活减毒FCV、根据本发明的LRCV或根据本发明的活减毒杂合FCV的细胞培养物。
如上所示，根据本发明和/或其实施方案的FCV衣壳蛋白针对各种不同FCV毒株提供广泛水平的交叉保护。
由于该原因，根据本发明和/或其实施方案的活减毒FCV，根据本发明和/或其实施方案的活重组载体病毒和根据本发明和/或其实施方案的活减毒杂合FCV为用于针对FCV保护猫的疫苗提供了非常合适的基础。
因此，本发明的又另一个实施方案涉及用于针对FCV保护猫的疫苗，其中此类疫苗包含根据本发明和/或其实施方案的活减毒FCV和药学上可接受的载体，和/或根据本发明和/或其实施方案的活减毒重组载体病毒和药学上可接受的载体，和/或根据本发明和/或其实施方案的活减毒杂合FCV和药学上可接受的载体。
保护在这方面应当在广义上解释：针对FCV保护猫被认为包含接种以预防疾病，接种以减少疾病的体征和在疾病被诊断后的治疗性接种。
适合于用于根据本发明使用的疫苗中的药学上可接受的载体的实例是无菌水、盐水、含水缓冲液诸如PBS等。此外，根据本发明的疫苗可以包含其他添加剂，诸如稳定剂和/或抗氧化剂。
如上所提及，从野外分离的FCV的毒力相对高：猫杯状病毒感染是上呼吸道感染的原因，并且当毒性FCV被口咽施用时，其引起发热、眼-鼻分泌物、龈口炎、舌炎、体重减轻和不佳身体状况。FCV的毒性全身形式引起发热、血管炎、水肿、四肢上的溃疡性病变、黄疸和死亡。(存在零星的信息：甚至FCV F9的疫苗毒株当口咽施用时确实引起龈口炎)。
如本文所定义的活减毒病毒是当与从野外分离的病毒相比具有降低水平的毒力的病毒。用根据本发明和/或其实施方案的活减毒病毒、活减毒杂合病毒或LRCV接种至少在发热持续时间、口腔溃疡、体重减轻和/或病毒分泌天数的方面当与用野生型FCV感染未接种的动物相比时降低感染的严重程度(临床体征和症状的减少)。
通常，可以在不添加佐剂的情况下使用基于活减毒FCV、LRCV和活减毒杂合FCV的疫苗。尽管如此，如果需要如此，疫苗中可以包括佐剂。
佐剂是以非特异性方式加强宿主的免疫应答的免疫刺激物质。所述佐剂可以是亲水性佐剂，例如氢氧化铝或磷酸铝，或疏水性佐剂，例如基于矿物油的佐剂。
根据本发明和/或其实施方案的基于活减毒FCV、LRCV和活减毒杂合FCV的疫苗可以包含稳定剂。可以将稳定剂添加至根据本发明和/或其实施方案的疫苗中，例如，以保护其免于降解、延长保质期或改善冷冻干燥效率。可用的稳定剂尤其是SPGA (Bovarnik等人,1950, J. Bacteriology, vol. 59, p. 509)，脱脂乳，明胶，牛血清白蛋白，碳水化合物，例如山梨醇，甘露醇，海藻糖，淀粉，蔗糖，葡聚糖或葡萄糖，乳糖，蛋白诸如白蛋白或酪蛋白或其降解产物，和缓冲液，诸如碱金属磷酸盐。为了重构冷冻干燥的组合物，将其悬浮于生理上可接受的稀释剂中。这种稀释剂可以例如与无菌水或生理盐溶液一样简单。以更复杂的形式，冷冻干燥的疫苗可以悬浮于乳剂中，例如如EP 1,140,152中所述。
用于将根据本发明和/或其实施方案的疫苗应用于靶生物体的剂量方案可以是单剂量或多剂量的应用并且以如免疫有效的量应用。
何者构成基于根据本发明和/或其实施方案的病毒的根据本发明的疫苗的“免疫原性有效量”取决于期望的效果。如本文所用的术语“免疫原性有效量”涉及根据本发明的活减毒FCV、活减毒载体病毒或活减毒杂合FCV在与由野生型FCV在未免疫猫中的感染引起的病理效果相比时其减少由野生型FCV病毒感染引起的病理效果的程度上在猫中诱导免疫应答所必需的量。
确定治疗是否是“免疫学有效的”，例如通过将实验性攻击感染施用于接种的动物和接下来确定靶动物的疾病临床体征、血清学参数或通过测量病原体的重新分离，随后将这些发现与攻击未接种的猫后观察到的那些进行比较，完全在技术人员的能力之内。
施用的病毒量将取决于施用途径、可能佐剂的存在和施用的时刻。
包含根据本发明和/或其实施方案的活减毒FCV或活减毒杂合病毒的活疫苗的优选量例如表达为组织培养感染剂量(TCID50)。例如，对于这种活减毒病毒，可以有利地使用每个动物剂量102至108 TCID50的剂量范围；优选使用104至106 TCID50的范围。
疫苗中基于粘液瘤病毒的活重组载体病毒的优选量将在104 – 108噬斑形成单位(PFU)的范围内。
疫苗中基于猫疱疹病毒的活重组载体病毒的优选量也将在104 – 108噬斑形成单位(PFU)的范围内。
可以应用几种施用方式，其都是本领域中已知的。根据本发明的疫苗优选经由注射(优选肌内注射)施用于猫。用于施用的方案可以根据标准FCV或活重组载体病毒接种实践进行优化。
驯化的猫通常针对几种疾病接种。出于易于施用的原因和还有经济原因，期望同时施用几种疫苗，优选作为组合疫苗。此类组合疫苗然后将包含根据本发明和/或其实施方案的活减毒FCV和/或根据本发明和/或其实施方案的活减毒杂合FCV和/或根据本发明和/或其实施方案的活重组载体病毒，并且除了这以外，包含至少一种其他猫致病性微生物或猫致病性病毒和/或所述猫致病性微生物或猫致病性病毒的至少一种其他免疫原性组分和/或编码所述猫致病性微生物或猫致病性病毒的所述其他免疫原性组分的遗传物质。
因此，该实施方案的优选形式涉及用于针对FCV保护猫的疫苗，其中此类疫苗包含根据本发明的活减毒FCV和药学上可接受的载体，和/或根据本发明的活重组载体病毒和药学上可接受的载体和/或根据本发明的活减毒杂合FCV和药学上可接受的载体以及至少一种其他猫致病性微生物或猫致病性病毒和/或所述猫致病性微生物或猫致病性病毒的至少一种其他免疫原性组分和/或编码所述猫致病性微生物或猫致病性病毒的所述其他免疫原性组分的遗传物质。
在该实施方案的一个更优选形式中，至少一种其他猫致病性微生物或猫致病性病毒选自猫全白细胞减少症病毒、鹦鹉热衣原体(Chlamydia psittaci)、支气管炎博德特氏菌(Bordetella bronchiseptica)、猫细小病毒、狂犬病病毒和猫疱疹病毒。
在实施例部分中，提供了构建根据本发明的活减毒杂合FCV的详细实例。基本上，该方法包括优选使用重叠延伸组装第一和第二扩增子(所述第一和第二扩增子各自包含全长病毒基因组的一部分)、产生包含全长病毒基因组的扩增子的步骤。
合适地，第一FCV扩增子包含减毒FCV的完整ORF1区域和ORF2区域的相邻5’-部分，且第二FCV扩增子包含ORF1区域的3’-部分和完全相邻的ORF2//ORF3区域，其中ORF2是编码根据本发明和/或其实施方案的FCV衣壳蛋白的ORF2。
因此存在跨越两个扩增子中均存在的ORF1区域的5’-部分和ORF2区域的3’-部分的重叠区域。这将允许通过重叠延伸来组装第一和第二扩增子。
因此，又另一个实施方案涉及用于获得根据本发明的活减毒杂合FCV的方法，其包括以下步骤：
a. 制备包含减毒FCV的完整ORF1区域和ORF2区域的相邻5’-部分的第一FCV扩增子，
b. 制备包含ORF1区域的3’-部分和完全相邻的ORF2//ORF3区域的第二FCV扩增子，其中ORF2是根据本发明的ORF2，
c. 使用重叠延伸组装第一和第二扩增子，
d. 生成感染性FCV，
e. 用感染性FCV感染易感细胞，
f. 回收感染性子代FCV。
附图说明
图1：用PCR从FCV cDNA扩增覆盖FCV基因组的5’末端起的5349bp或FCV F9和KalemCrouch基因组分别的3’末端起的2422和2416bp的扩增子。
图2：生成FCV F9 (SEQ ID NO:60)、Kalem Crouch (SEQ ID NO:59)、FK (SEQ IDNO:62)和KF (SEQ ID NO:61)的全长重叠延伸组装体，并在1％琼脂糖凝胶上分辨。FCV F9和Kalem Crouch从它们各自的5’和3’扩增子制备作为对照，以证明正确的重叠设计。
图3：用PCR扩增全长重组FK和KF FCV DNA，并在1％琼脂糖凝胶上分辨。
图4：被FCV Kalem Crouch感染的CrFK细胞中FCV的典型CPE(细胞病变效应)的实例。
图5：FCV Kalem Crouch (SEQ ID NO:34)和F9 (SEQ ID NO:35)衣壳蛋白序列与公开的FCV序列(SEQ ID NO:36-58)的比对。氨基酸的编号在核苷酸水平上。
图6：FCV F9 (SEQ ID NO:59)和Kalem Crouch毒株(SEQ ID NO:60)与重组FCV FK(SEQ ID NO:61)和KF毒株(SEQ ID NO:62)的序列比对。
图7：FCV Kalem Crouch (SEQ ID NO:34)和F9 (SEQ ID NO:35)衣壳蛋白序列与公开的FCV序列(SEQ ID NO:36-58)的比对。氨基酸的编号在氨基酸水平上。
图8：在所示的MCS的NcoI位点中具有突变的p22m-GFP质粒的图谱。
图9：源自p22-GFP质粒的p22m-GFP和p22m-4a构建体的比较。
图10：具有突出的pMCPK插入物的整个MR24-Kalem Crouch克隆的基因组的图。
具体实施方式
实施例1：
使用在猫中产生的针对毒株FCV F9和Kalem Crouch的超免疫血清来测定几种FCV毒株的中和指数。该实验如下面部分8中所述进行。数据显示于表1中。从表中变得清楚的是，针对Kalem Crouch产生的血清针对许多其他FCV毒株具有广泛的交叉保护。对于针对Kalem Crouch的血清，针对31种FCV毒株中的16种看到显著的Log10降低(即> 1.5)。表1还显示通常使用的F9毒株的交叉保护低得多。对于22种FCV毒株中的3种，针对F9产生的血清显示显著的Log10降低(即>1.5)。应当注意的是，被F9血清中和或至少显著降低的2种FCV毒株3809，6420，CV-21是F9样病毒。因此，不仅为比F9多得多的FCV毒株提供了Kalem Crouch交叉保护，而且其为非F9毒株提供了交叉保护。
表 1：通过血清的Log10降低
Figure 558071DEST_PATH_IMAGE001
实施例2
杂合FCV-克隆的构建。
1. 细胞培养
所有细胞系都在37℃、5% CO2下维持在组织培养烧瓶中。
使Crandell-Rees猫肾(CrFK)细胞在补充有5％胎牛血清、0.15％碳酸氢钠、2mML-谷氨酰胺、100U/ml青霉素、10μg/ml链霉素和2μg/ml两性霉素B的培养基M6B8中生长。
将BsRT7细胞维持在补充有5％胎牛血清、2mM L-谷氨酰胺、1mM丙酮酸钠和1mg/ml遗传霉素(G418)的培养基DMEM中。转染前在细胞接种时移除遗传霉素。
2. 病毒分离
从猫收集口咽/鼻拭子并转运在培养基M6B8中。将拭子短暂涡旋并将病毒悬浮液接种至汇合的CrFK细胞上，并在37℃和5% CO2下孵育，直至观察到FCV特异性的CPE。将感染的烧瓶冻融以裂解细胞，澄清以移除细胞碎片并在-70℃下作为等分试样储存。
3. FCV的生长
吸附适当的病毒稀释液以感染汇合的CrFK单层。将细胞在37℃、5% CO2下孵育，直至观察到FCV特异性的CPE。将感染的烧瓶冻融以裂解细胞，澄清以移除细胞碎片并在-70℃下作为等分试样储存。
4. RNA分离
将澄清的病毒悬浮液在131500 x g、4℃下使用SW28转子离心近似16小时。使用RNeasy® Miniprep试剂盒(Qiagen, Hilden, Germany)从所得沉淀中提取RNA。将RNA洗脱于50μl无RNA酶的水中，等分并在-70℃下储存，直至使用。
5. cDNA合成
FCV RNA用作cDNA合成的模板。使用INVITROGEN Superscript II®试剂盒(Carlsbad, CA)和引物Fr2F (SEQ ID NO:32)和Fr4R (SEQ ID NO:33)合成cDNA。
6. 病毒滴定
使用在生长培养基中病毒的系列十倍稀释液(100μl/孔，每个稀释度5个孔)来感染96孔板中的CrFK细胞的汇合单层。将感染的CrFK细胞在37℃、5% CO2下孵育最长达5天，并检查FCV特异性的CPE。记录存在CPE的孔的数目并使用Reed Muench方法计算滴度。滴度表示为TCID50/ml。
7. FCV抗体的制备
在猫中产生针对FCV毒株的抗体。每个处理组由分别圈养的3只猫组成。猫通过口咽途径或通过皮下注射感染。将猫通过口咽途径用第二剂量的病毒超免疫。在第二次接种后三周从猫收集血浆。
8. 病毒中和测定
将病毒的连续稀释液与等体积的恒定量的血浆稀释液或生长培养基混合，并在37℃下孵育1小时。将病毒或病毒血清混合物接种在96孔板中的汇合CrFK细胞上(每个稀释度5个孔)。将板在37℃、5% CO2下孵育5天。通过计算观察到的滴度的差异来测定中和指数。
9. 用于从FCV cDNA生成重叠DNA扩增子的引物的设计
使用Phusion聚合酶(NEB, Ipswich, MA)与寡核苷酸引物对FKP1F (SEQ ID NO:5)和FKP1R (SEQ ID NO:6)以及表4中所述的PCR条件进行PCR反应以生成覆盖从FCV基因组的5’起的5349bp的扩增子。类似地，还使用Phusion聚合酶(NEB, Ipswich, MA)与寡核苷酸引物对FKP2F (SEQ ID NO:7)和FKP2R (SEQ ID NO:8)使用Phusion聚合酶(NEB, Ipswich,MA)和表5中所述的PCR条件进行PCR反应以生成覆盖从FCV F9的3’末端起的2422bp以及从FCV Kalem Crouch的3’末端起的2416 bp的扩增子。
10. 从PCR反应纯化DNA
使用QIAquick® PCR纯化试剂盒(Qiagen, Hilden, Germany)使用两次柱洗涤纯化所有扩增的DNA。使用Nanodrop仪器(Thermo Scientific, Waltham, MA)测定洗脱的DNA的浓度和纯度。
11. 组合FCV扩增子以生成全长FCV
制备与0.1、0.25或0.5pmol的每种FCV扩增子的等摩尔混合物，所述FCV扩增子如方法部分9中所述生成并且如方法部分10中所述进行纯化。使用足量的这种混合物(通常5μL)作为模板用于使用Phusion聚合酶(NEB, Ipswich, MA)的表6中描述的重叠延伸PCR。
12. 生成全长感染性FCV DNA
使用足量的如上面部分5中所述制备的cDNA反应物或如部分11中所述制备的重叠延伸PCR反应物(通常在1和5μL之间)作为模板以生成全长感染性FCV DNA。分别使用ThPhusion聚合酶(NEB, Ipswich, MA)连同寡核苷酸引物对MBL 446 (SEQ ID NO:1)和MBL447 (SEQ ID NO:2)或FCVT7f (SEQ ID NO:3)和FCVpAr (SEQ ID NO:4)以及表7和8中描述的PCR条件。
13. 将全长感染性FCV DNA转染至BsRT7细胞中
用如部分12中所述生成的全长感染性FCV DNA使用INVITROGEN® Lipofectamine® 3000转染如部分1中所述在24孔板中培养至近似50-70%汇合度的BsRT7细胞。通常每个孔使用3μg DNA。将细胞与DNA-脂质体复合物孵育最长达72小时。
14. 用来自转染的BsRT7细胞的裂解物感染CrFK细胞
通过冻融裂解转染的BsRT7细胞。细胞裂解物用于感染汇合的CrFK细胞单层。
15. FCV的免疫荧光染色
用FCV感染的CrFK细胞用甲醇固定并用PBS洗涤。将固定的细胞依次与多克隆抗FCV血清和抗猫FITC抗体缀合物或小鼠单克隆抗体NCL-1G9 (Leica Microsystems, UK)和抗小鼠FITC抗体缀合物孵育。使用具有I3滤光片的DM1L显微镜(Leica Microsystems, UK)观察荧光。
16. FCV的序列分析
使用寡核苷酸引物MBL 446 (SEQ ID NO:1)和MBL 447 (SEQ ID NO:2)连同Phusion聚合酶(NEB, Ipswich, MA)以及表4中所述的PCR条件从cDNA制备全长FCV DNA。所得全长FCV DNA如方法部分11中所述纯化，并使用来自表3的寡核苷酸引物的任何组合进行测序。DNA样品通过GATC-biotech, UK进行测序。30-100 ng/µl的质粒或10-50 ng/µl的PCR产物与10 pmol/µl的测序引物一起发送。
表1用于生成全长感染性FCV DNA的PCR引物。
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表2 用于生成FCV扩增子的PCR引物。
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表3 用于测序重组FCV基因组的全长的PCR引物。
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表4 用于生成FCV的覆盖从5’末端起5349 bp的扩增子的PCR条件。
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表5 用于生成FCV的覆盖从3’末端起最长达2422 bp的扩增子的PCR条件。
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表6进行组合FCV扩增子以生成全长FCV DNA模板的重叠延伸PCR的PCR条件。
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表7 用于从cDNA扩增全长FCV DNA并添加5’ T7启动子和3’多聚A束的PCR条件。
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表8 用于从重叠延伸PCR模板材料扩增全长FCV DNA并添加5’ T7启动子和3’多聚A束的PCR条件。
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2. Myxo-Kalem Crouch构建体的制备
在p22m-GFP (p22-GFP的衍生物)质粒MCS (多克隆位点)上使用BamHI和XhoI位点克隆pMCPK (Kalem Crouch FCV分离株的主要衣壳蛋白的加工部分)。参见图8。
为了避免向pMCPK添加额外的C-端AA(氨基酸)，通过引入点突变(CCATGG->CCATCG，图1)移除p22-GFP中的MCS的NcoI位点中从起始密码子的翻译。定点诱变用于使用以下引物突变p22-GFP质粒：-
Figure 795597DEST_PATH_IMAGE011
PCR程序：在98℃，30”，20x[在98℃，10”, 在58.3℃，10”，在72℃，2’], 在72℃，5’，在4℃，∞，和Phusion聚合酶(NEB，目录号：M0530L)。
在转化至XL10 gold大肠杆菌(目录号：200315)中前，用DpnI消化将模板p22-GFP质粒从反应中移除。挑取所得大肠杆菌菌落中的几个以建立小量制备培养物，其通过用NcoI消化提取的质粒DNA (QiaPrep Spin Miniprep试剂盒，目录号：27104)来筛选。在1％琼脂糖凝胶上，对应于线性化质粒的独特条带表明成功突变(因为p22m-GFP质粒中唯一剩余的NcoI位点存在于GFP基因的上游)。除了消化后的线性化质粒之外，342bp片段表明存在两个NcoI位点，且因此存在完整的p22-GFP质粒。随后使用测序来证实突变。
插入物pMCPK使用PCR制备，其用以下引物：-
Figure 532609DEST_PATH_IMAGE012
其在pMCPK的5’末端的Kozac序列中包括起始密码子。所用的PCR程序是：在98℃，30”，35x[在98℃，10”，在55℃，10”，在72℃，40”]，在72℃，5’，在4℃，∞，而模板是从分离自被Kalem Crouch FCV感染的CRFK细胞的总RNA制备的cDNA。在1％琼脂糖凝胶上证实正确的扩增子大小后，将PCR反应中的插入物DNA纯化(Qiagen PCR清洁试剂盒)并用BamHI和XhoI与p22m-GFP质粒平行消化。连接后，该程序导致用pMCPK替代GFP插入物和5’和3’ RHDV重复侧翼(图2)。将消化的插入物和质粒DNA上样至1％琼脂糖凝胶上，并切下1664bp(插入物)和4031bp(质粒骨架)的条带并使用StrataPrep DNA凝胶提取试剂盒(目录号：400766)纯化。将骨架和插入物在4℃下连接过夜，且随后将2uL该连接物转化至XL10 gold大肠杆菌(目录号：200315)中。建立小量制备培养物，并通过用BamHI和XhoI两者消化来筛选提取的DNA。通过使用与生成pMCPK插入物的相同PCR反应(参见上文)和随后测序证实插入物的身份。选择构建体p22m-4a用于随后的步骤中。
经5小时将1mL M6B8 + 5％FBS培养基中稀释的50μL MR24材料应用至具有~80%汇合的RK13细胞的6cm皿中，然后洗去所有未吸收的MR-24病毒，补充额外3mL相同的培养基，并用~4.5ug p22m-4a质粒使用Lipofectamine 3000转染。~17小时后，通过温和刮取收获部分细胞并与培养基一起保存。将板上剩余的一半细胞固定(100％ EtOH)，并用FCV-抗血清、随后用FITC-标记的抗猫抗体针对免疫荧光染色，以证实pMCPK的表达。染色的细胞表明pMCPK表达增强以及p22m-4a和MR-24之间可能重组以产生MR-24-Kalem Crouch，因为仅用p22m-4a转染或仅用MR-24感染的对照细胞没有染色(对于重组MR24-Kalem Crouch病毒的图表，参见图10)。
通过连续轮的滴定、表达的pMCPK的免疫荧光检测和富集样品的稀释来进行MR-24-Kalem Crouch重组粘液瘤病毒的富集。简而言之，以一定范围的稀释度用来自感染/转染的病毒感染一系列用RK-13细胞接种的96-孔组织培养皿。3天后，将所有96-孔皿都冷冻并保留为第一轮储备物。第二系列的RK-13接种的96-孔皿然后用来自第一轮储备物的材料(来自每个孔5-10μl)感染。2-3天后，将这些重复皿用冰冷的甲醇固定，并首先用猫抗FCV多克隆抗血清染色，且然后用山羊抗猫IgG FITC标记的第二抗体染色。鉴定含有荧光的感染灶的孔并取出第一轮储备物皿上相应的孔，然后稀释并用于感染第二系列的96-孔皿，其变为第二轮储备物。重复该程序，直至病毒储存物含有大部分重组病毒。通过三轮单灶分离实现最后的纯化。扩增三个最佳染色克隆(即B8、A9和A10)，并将克隆A9用于进一步实验以测定克隆纯度(即在背景中缺乏野生型MR24生长)和插入物(即pMCPK)序列稳定性。将MR24-Kalem Crouch通过每次以0.001 MOI接种而在RK13细胞中传代5次。
为了确定pMCPK插入物的稳定性，比较了来自第1代的MR24-Kalem Crouch DNA和来自第5代的MR24-Kalem Crouch DNA。在p22m-4a相比于MR24-Kalem Crouch-第1代或MR24-Kalem Crouch-第1代相比于MR-24-Kalem Crouch-第5代中未检测到突变，表明p22m-4a中的pMCPK与MR24成功重组并且经病毒的5代保持稳定。
总之，这些实验显示，FCV Kalem Crouch (pMCPK)的加工的主要衣壳蛋白已被插入MR24-Kalem Crouch克隆A9的MGF位点中并从其表达。
结果
1. FCV Kalem Crouch的分离和生长
猫杯状病毒(FCV)毒株Kalem Crouch分离自在2010年12月在Jersey的FCV爆发期间所取的拭子。该拭子源自一只阉割的雄性，2岁6个月，命名为Kalem Crouch，并且由NewEra Veterinary Surgery, St Saviour, Jersey收集。将拭子短暂涡旋并将病毒悬浮液接种至汇合的CrFK细胞上，并在37℃和5% CO2下孵育，直至观察到FCV特异性的CPE。将感染的烧瓶冻融以裂解细胞，澄清以移除细胞碎片并在-70℃下储存。病毒的滴度为106.91 TCID50/ml。
测定分离株的核苷酸序列。该序列在SEQ ID NO:60中注释。
衣壳蛋白的氨基酸序列与公共领域中可用的其他FCV序列进行比对。序列比对在图5和7中注释。
2. 生成重组FCV病毒
2.1. FCV扩增子的制备
如方法部分5中所述制备的FCV F9或Kalem Crouch cDNA用作PCR反应中的模板，所述PCR反应用Phusion聚合酶(NEB, Ipswich, MA)、寡核苷酸引物对FKP1F (SEQ ID NO:9)和FKP1R (SEQ ID NO:10)以及表4中描述的条件以生成覆盖FCV基因组的5’末端起的5349bp的扩增子。类似地，使用寡核苷酸引物对FKP2F (SEQ ID NO:11)和FKP2R (SEQ IDNO:12)以及表5中所述的PCR条件来生成覆盖FCV F9的3’末端起的2422 bp和FCV KalemCrouch的3’末端起的2416 bp的扩增子。通过经1小时以120V在1 x TBE缓冲液(Sigma-Aldrich, St. Louis, MO)中进行电泳来解析这些扩增子和5μL GeneRuler 1kb Plus DNA梯(Thermo Scientific, Waltham, MA)。预期大小的条带显示于图1中。
2.2.使用重叠延伸PCR来组装FCV扩增子
使用QIAquick® PCR纯化试剂盒(Qiagen, Hilden, Germany)纯化结果部分2中生成的FCV扩增子。这些扩增子用于制备杂合病毒：杂合病毒FK在F9背景中包含KalemCrouch衣壳，且杂合病毒KF在Kalem Crouch背景中包含F9衣壳。
为了制备FCV FK和KF模板DNA，用FCV F9 5’末端和FCV Kalem Crouch 3’末端扩增子或FCV Kalem Crouch 5’末端和FCV F9 3’末端扩增子制备含有0.1至0.5pmol每种扩增子的等摩尔混合物。这些混合物用作具有表6中所述的条件的重叠延伸PCR反应中的模板。组装的FK和KF DNA扩增子的预期大小分别为7685bp和7702bp。通过经1小时以120V在1x TBE缓冲液(Sigma-Aldrich, St. Louis, MO)中进行电泳来解析这些样品中组装的DNA和5μL GeneRuler 1kb Plus DNA梯(Thermo Scientific, Waltham, MA)。所得组装的F9、Kalem Crouch、FK和KF的DNA显示于图2中。
2.3. 感染性FCV病毒的生成
使用Phusion聚合酶(NEB, Ipswich, MA)和寡核苷酸引物对FCVT7f (SEQ ID NO:3)和FCVpAr (SEQ ID NO:4)用表8中描述的PCR条件制备感染性FCV FK或KF DNA。感染性FCV FK和KF DNA的预期大小分别为7728bp和7737bp。通过经1小时以120V在1 x TBE缓冲液(Sigma-Aldrich, St. Louis, MO)中进行电泳来解析这些样品中的感染性FCV DNA和5μLGeneRuler 1kb Plus DNA梯(Thermo Scientific, Waltham, MA)。FK和KF的全长感染性DNA分别显示于图3部分A和B中。
2.4. 感染性FCV病毒的回收
使用QIAquick® PCR纯化试剂盒(Qiagen, Hilden, Germany)从全长PCR反应物纯化感染性FCV FK和KF DNA，并使用Invitrogen® Lipofectamine® 3000试剂(Carlsbad,CA)如方法部分13中所述转染至在24-孔板上生长的50-90％汇合的BsRT7细胞上。转染的BsRT7细胞在正常生长条件下与转染复合物一起孵育24-72小时，然后通过冻融裂解。然后将来自每个孔的BsRT7裂解物应用至良好生长至50和100％之间的汇合度的CrFK细胞。在正常生长条件下孵育在BsRT7细胞裂解物存在的情况下生长的CrFK细胞，如方法部分1中所述。通常通过在CrFK细胞的单层中形成噬斑来检测病毒的存在，其类似于图4中所示的那些。
2.5. FCV FK和KF病毒的序列。
将重组FCV病毒进行测序。
已经将这些序列与图6中的FCV F9和Kalem Crouch的序列进行比较。重组FK病毒被表示为SEQ ID NO:61并且包含Kalem Crouch衣壳。重组KF病毒被表示为SEQ ID NO:62并且包含F9衣壳。
Myxo-Kalem Crouch构建体在猫中的效力
实验设计：
将15只8-11周龄的短毛家猫分成两组。一组10只猫，其用上述重组Myxo-KalemCrouch构建体(第5代)(每个剂量106.23 TCID50)间隔三周皮下接种两次；和一组5只对照猫。第二次接种后四周，将猫取拭子并且对照未接种的猫中的两只用毒性FCV毒株KalemCrouch(每只猫104.0 TCID50)鼻内攻击并与剩余的猫混合用于接触攻击。从攻击后的第1天到攻击后的第17天，每天对所有猫取拭子。记录临床观察结果，包括体重和体温。临床发现如下评分，参见表8。(施用退热剂以缓解发热和痛苦。在先前的实验中，证明了退热剂的施用对病毒分泌没有影响)。
表 8：临床体征的评分的概述
临床体征 评分
轻度不适(MA+) 1
明显不适(Ma++) 2
存在溃疡(无论数量或大小) 1
跛/跛行(无论患肢的数量) 2
病毒脱落 1
发热(温度高于≥39.5℃) 1
施用退热剂以缓解发热和痛苦(当温度高于40℃时施用) 10 (每次施用)
与前一天相比体重减轻 1
结果：
猫在接种前没有抗体(第-1天)。在猫中在接种后(第48天)没有观察到强烈的血清-转化。在猫中在攻击后(第66天)观察到强烈的血清-转化。
表 9：抗体的滴度
Figure 55994DEST_PATH_IMAGE013
在实验开始时或攻击前一天，不能从猫分离病毒。可以从第1组和第2组的所有猫分离病毒，在属于两组的猫中观察到与FCV相关的临床体征，表明实质性攻击。
表 10：临床评分
Figure 485838DEST_PATH_IMAGE014
Figure 806092DEST_PATH_IMAGE015
表 11：每组的临床体征：
Figure 81216DEST_PATH_IMAGE016
对数据的Kruskal-Wallis非参数检验显示接种的猫和对照猫之间的总评分(P =0.037)和发热评分(P = 0.020)的统计学显著差异，表明Myxo-Kalem Crouch构建体能够针对FCV攻击感染诱导免疫(攻击后猫的临床评分的减少)。
实验设计
2.7 产生针对FCV的FK和KF杂合病毒的超免疫血清的研究。
该研究包括六只229至432日龄的短毛家猫。这些被分成两组，每组3只猫，其中组之间相对均匀分开雄性猫。每组分开圈养。适应后，用104.6 TCID50/剂量的FCV毒株FK皮下接种属于组1的猫。用104.6 TCID50/剂量的FCV毒株KF皮下接种属于组2的猫。
然后所有猫在两周后(第14天)以105 TCID50/剂量鼻内接受第二剂量的相同病毒。在第二次接种后三周收集血清。
将血清热灭活并进行病毒中和测试。通过病毒诱导的CrFK细胞上的细胞病变效应(CPE)的减少来评价病毒中和。将32-316 TCID50的病毒的5倍重复与等体积的血清的连续稀释液(从1:4开始)混合。然后将病毒/血清混合物在37℃下孵育至少60分钟。然后将100μl病毒-血清混合物添加至用100μl生长培养基中的CrFK细胞接种的96-孔组织培养皿中。连续孵育5天。VN滴度被表示为病毒诱导的CPE完全不存在时的最高血清稀释度的倒数。
结果
表 12：首次接种(s.c.)后的VN滴度
Figure 724687DEST_PATH_IMAGE017
NT：未测试。
表 13：第二次接种(i.n.)后的VN滴度
Figure 59853DEST_PATH_IMAGE018
数据显示重组病毒FK和KF在猫中是免疫原性的。猫中发展的抗体是功能性的(中和的)。抗体的交叉反应性显示与FCV毒株F9和FCV毒株Kalem Crouch之间观察到的层次相似的层次，使得用杂合毒株KF (F9衣壳)接种猫诱导针对毒株F9、但不针对毒株KalemCrouch的病毒中和抗体，而用杂合毒株FK (Kalem Crouch衣壳)接种导致诱导针对毒株F9和Kalem Crouch的病毒中和抗体。
2.8中和指数
使用在猫中产生的针对毒株FCV F9和Kalem Crouch的超免疫血清来测定重组FCV毒株FK和KF的中和指数。数据显示于表7中。
从表中变得清楚的是，FCV毒株FK确实被抗FCV Kalem Crouch超免疫血清强烈中和，而FCV毒株KF确实被抗FCV F9超免疫血清强烈中和。
Figure 664141DEST_PATH_IMAGE019
表14 FCV F9和Kalem Crouch抗血清针对重组FCV FK和KF的中和指数。
表14和获得自从FK和KF接种的猫生成的血清的数据(表13)表明存在病毒中和的单向层次。F9和KF (F9衣壳)抗血清没有有效地中和Kalem Crouch或FK (Kalem Crouch衣壳)病毒，而来自用Kalem Crouch和FK (Calem Crouch衣壳)接种的猫的血清中和自身并且也显著中和F9和KF (F9衣壳)病毒。
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<213> 猫杯状病毒
<400> 27
gcccaaacta tgaaacaag 19
<210> 28
<211> 20
<212> DNA
<213> 猫杯状病毒
<400> 28
aacgccattg gatctgtaac 20
<210> 29
<211> 20
<212> DNA
<213> 猫杯状病毒
<400> 29
attgaaccaa tcgatcctga 20
<210> 30
<211> 20
<212> DNA
<213> 猫杯状病毒
<400> 30
tcaggatcga ttggttcaat 20
<210> 31
<211> 32
<212> DNA
<213> 猫杯状病毒
<400> 31
ttccaggtac ctccggaagg agttctgggt ag 32
<210> 32
<211> 53
<212> DNA
<213> 猫杯状病毒
<400> 32
agagctctct ggctaacgta aaagaaattt gagacaatgt ctcaaactct gag 53
<210> 33
<211> 34
<212> DNA
<213> 猫杯状病毒
<400> 33
ggcaactaga aggcacagcc ctggggttag gcgc 34
<210> 34
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 34
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Lys Leu Val Ile Asp Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Lys Met Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Met Leu Met His His Ile Ile Gly Glu Val Ala Lys
100 105 110
Gly Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Val Thr Thr Pro Glu Gln Gly Thr Met Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Ser Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Ile Ser Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Ile Pro Asp Leu Arg
260 265 270
Asn Ser Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Ile Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Ile Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Leu Ala Val Thr Val Ser Gln Ser Lys Gly
385 390 395 400
Ala Lys Leu Gly Asn Gly Ile Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Thr Lys Leu Thr Pro Thr
420 425 430
Gly Asp Tyr Ala Ile Thr Ser Ser Asp Gly Asn Asp Ile Glu Thr Lys
435 440 445
Leu Glu Tyr Glu Asn Ala Asp Val Ile Lys Asn Asn Thr Asn Phe Arg
450 455 460
Ser Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Gly Phe Ile Thr Thr Gly Val Ile Ser Asp Asn Ser
485 490 495
Ile Ser Pro Ser Asn Thr Ile Asp Gln Ser Lys Ile Val Val Tyr Gln
500 505 510
Asp Asn His Val Asn Ser Glu Val Gln Thr Ser Asp Ile Thr Leu Ala
515 520 525
Ile Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asn Arg
530 535 540
Asp Ser Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Met Lys Leu Gly Tyr Val
565 570 575
Ile Ser Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn Asn Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Val Ser Ser Phe Pro Lys Leu Glu
625 630 635 640
Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 35
<211> 671
<212> PRT
<213> 猫杯状病毒
<400> 35
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Lys Leu Val Ile Asn Pro Asn Asn Phe Leu Ser Val Gly Phe
20 25 30
Cys Ser Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Arg Ser Pro Leu Glu Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Phe Asp Ala Val Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Gly Ala Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Gly Lys Val Ala Ala
100 105 110
Gly Trp Asp Pro Asp Leu Pro Leu Ile Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Ala Pro Glu Gln Gly Thr Met Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Cys Leu Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ala Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Ile Pro Ser Asp Leu Ile Pro Lys Thr Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Tyr Trp Ser Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Ser Val Thr Ile Thr Glu Gln Asn Gly
385 390 395 400
Ala Lys Leu Gly Ile Gly Val Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Gly Glu Leu Ile Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Thr Asn Gly Thr Gly Asn Asp Ile Thr Thr Ala
435 440 445
Thr Gly Tyr Asp Thr Ala Asp Ile Ile Lys Asn Asn Thr Asn Phe Arg
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Thr Leu Asp Gly Asp Asn
485 490 495
Asn Asn Lys Ile Asn Pro Cys Asn Thr Ile Asp Gln Ser Lys Ile Val
500 505 510
Val Phe Gln Asp Asn His Val Gly Lys Lys Ala Gln Thr Ser Asp Asp
515 520 525
Thr Leu Ala Leu Leu Gly Tyr Thr Gly Ile Gly Glu Gln Ala Ile Gly
530 535 540
Ser Asp Arg Asp Arg Val Val Arg Ile Ser Thr Leu Pro Glu Thr Gly
545 550 555 560
Ala Arg Gly Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu
565 570 575
Gly Tyr Val Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His
580 585 590
Thr Ser Arg Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser
595 600 605
Phe Ala Val Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile
610 615 620
Gly Ile Asp Ser Asp Gly Phe Ser Phe Val Gly Val Ser Gly Phe Gly
625 630 635 640
Lys Leu Glu Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala
645 650 655
Lys Ile Arg Leu Ala Ser Asn Ile Arg Ser Pro Met Thr Lys Leu
660 665 670
<210> 36
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 36
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Lys Leu Ile Ile Asn Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asn Cys Asn Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Val Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asn Glu Ala Gly Lys Leu Phe Gln
85 90 95
Pro His Pro Gly Ile Leu Met His His Ile Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Pro Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Ala Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Ser His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Ile Val Pro Pro Gly Ile
225 230 235 240
Glu Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Asn Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Ile Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Ala Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Ile Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Thr Asp Ile Thr Glu Phe Val Val Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Val Thr Val Ser Glu Lys Gly Gly
385 390 395 400
Ser Lys Leu Gly Ile Gly Val Ala Thr Asp Ser Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Ser Ile Ser Asp Thr Leu Ile Pro Ser
420 425 430
Gly Asp Tyr Ala Ile Thr Ser Gly Asn Gly Asn Asp Ile Ile Thr Gly
435 440 445
Ala Asp Tyr Asp Ser Ala Asp Val Ile Lys Asn Asn Thr Asn Phe Arg
450 455 460
Ser Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Val Ser Glu Thr Ala Phe Ile Thr Thr Ala Val Arg Asn Glu Asn Ser
485 490 495
Ile Ser Pro Ser Asn Val Ile Asp Ala Thr Lys Leu Ile Val Tyr Gln
500 505 510
Asp Ala His Val Glu Thr Asp Val Gln Thr Ser Asp Val Thr Leu Ala
515 520 525
Ile Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Leu Asp Arg
530 535 540
Asp Lys Val Val Arg Ile Ser Ile Leu Pro Glu Thr Gly Thr Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Met Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn Asn Tyr Ser Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Ala Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Ile Ser Asn Val Gly Lys Leu Ile
625 630 635 640
Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ala Met Thr Lys Leu
660 665
<210> 37
<211> 669
<212> PRT
<213> 猫杯状病毒
<400> 37
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Arg Leu Thr Ile Asn Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asn Gln Ser Pro Leu Glu Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Cys Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Ser Glu Val Ala Lys
100 105 110
Gly Trp Asp Pro Asn Leu Pro Ser Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Pro Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Ser Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Gly Lys Ala Phe Phe Ser Phe His Thr Ser
165 170 175
Val Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln
180 185 190
Ser Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ala Lys Leu
195 200 205
Tyr Val Ala Trp Ser Gly Ser Val Glu Val Arg Phe Ser Ile Ser Gly
210 215 220
Ser Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly
225 230 235 240
Val Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu
245 250 255
Phe Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu
260 265 270
Arg Asn Ser Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu
275 280 285
Val Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Ser Asp Ser
290 295 300
Asn Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp
305 310 315 320
Phe Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly
325 330 335
Ser Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly
340 345 350
Asn Arg Tyr Trp Ser Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val
355 360 365
Phe Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp
370 375 380
Ser Thr Pro Arg Phe Arg Pro Leu Thr Ile Thr Ile Ser Val Glu Gly
385 390 395 400
Gly Ala Lys Leu Gly Thr Gly Val Ala Thr Asp Tyr Ile Val Pro Gly
405 410 415
Ile Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Glu Lys Leu Thr Pro
420 425 430
Ala Gly Asp Tyr Ala Ile Thr Asp Gly Asn Gly Ser Asp Ile Val Thr
435 440 445
Ala Ala Lys Tyr Asp Thr Ala Asp Val Ile Lys Asn Asn Thr Asn Phe
450 455 460
Lys Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys
465 470 475 480
Lys Ile Ser Asn Thr Ala Phe Ile Thr Thr Gly Thr Val Glu Asn Asn
485 490 495
Ser Ile Glu Pro Ser Asn Thr Ile Asp Ala Thr Lys Leu Ala Val Phe
500 505 510
Gln Asp Thr His Val Gly His Glu Val Gln Thr Ser Asp Asp Thr Leu
515 520 525
Ala Leu Leu Gly Tyr Thr Gly Ile Gly Glu Gln Ala Ile Gly Ser Asp
530 535 540
Arg Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg
545 550 555 560
Gly Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr
565 570 575
Val Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser
580 585 590
Arg Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser Phe Ala
595 600 605
Val Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile
610 615 620
Asp Ser Asp Gly Phe Ser Phe Val Gly Val Ser Ser Ile Thr Lys Leu
625 630 635 640
Glu Phe Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile
645 650 655
Arg Leu Ala Ser Asn Ile Arg Gly Gln Met Thr Lys Ile
660 665
<210> 38
<211> 669
<212> PRT
<213> 猫杯状病毒
<400> 38
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Arg Leu Thr Ile Asn Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Glu Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Cys Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Ser Glu Val Ala Lys
100 105 110
Gly Trp Asp Pro Asn Leu Pro Ser Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Pro Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Ser Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Gly Lys Ala Phe Phe Ser Phe His Thr Ser
165 170 175
Val Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln
180 185 190
Ser Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ala Lys Leu
195 200 205
Tyr Val Ala Trp Ser Gly Ser Val Glu Val Arg Phe Ser Ile Ser Gly
210 215 220
Ser Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly
225 230 235 240
Val Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu
245 250 255
Phe Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu
260 265 270
Arg Asn Ser Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu
275 280 285
Val Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Ser Asp Ser
290 295 300
Asn Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp
305 310 315 320
Phe Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly
325 330 335
Ser Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly
340 345 350
Ile Cys Leu Trp Arg Gly Ile Thr Asp Phe Val Ile Arg Pro Phe Val
355 360 365
Phe Gln Val Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp
370 375 380
Ser Thr Pro Arg Phe Arg Pro Leu Thr Ile Thr Ile Ser Val Lys Glu
385 390 395 400
Gly Ala Asn Ser Glu Leu Gly Val Ala Thr Asp Tyr Ile Val Pro Gly
405 410 415
Ile Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Glu Lys Leu Thr Pro
420 425 430
Ala Gly Asp Tyr Ala Ile Thr Asp Ser Ser Gly Ser Asp Ile Thr Thr
435 440 445
Ala Ala Lys Tyr Asp Ala Ala Asp Val Ile Glu Asn Asn Thr Asn Phe
450 455 460
Lys Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys
465 470 475 480
Lys Ile Ser Asn Thr Ala Phe Ile Thr Thr Gly Thr Val Glu Asp Asn
485 490 495
Phe Ile Glu Pro Ser Asn Thr Ile Asp Ala Thr Lys Leu Ala Val Phe
500 505 510
Gln Asp Thr His Val Gly Arg Glu Val Gln Thr Ser Asp Asp Thr Leu
515 520 525
Ala Leu Leu Gly Tyr Thr Gly Ile Gly Glu Gln Ala Ile Gly Ser Asp
530 535 540
Arg Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg
545 550 555 560
Gly Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr
565 570 575
Val Val Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser
580 585 590
Arg Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser Phe Ala
595 600 605
Val Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile
610 615 620
Asp Ser Asp Gly Phe Ser Phe Val Gly Val Ser Ser Ile Thr Lys Leu
625 630 635 640
Glu Phe Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile
645 650 655
Arg Leu Ala Ser Asn Ile Arg Ser Gln Met Thr Lys Ile
660 665
<210> 39
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 39
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asn Trp Asp Pro
1 5 10 15
His Phe Lys Leu Val Ile Asn Pro Asn Lys Phe Leu Pro Ile Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Lys Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His Tyr Ile Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Val Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ser Gln Met Ser Thr Ala Ala Asp Met Ala Ser Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ser Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Ile Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Val Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Ser Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Val Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Arg Thr Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Phe Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Val Thr Ile Ser Glu Lys Asp Gly
385 390 395 400
Ala Lys Leu Gly Ile Gly Val Ala Thr Asp Phe Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Gly Glu Arg Leu Val Pro Val
420 425 430
Gly Asp Tyr Ala Ile Thr Asn Ser Ala Gly Ile Asp Ile Thr Thr Ala
435 440 445
Ser Gln Tyr Asp Thr Ala Gly Val Ile Lys Asn Asn Thr Asn Phe Arg
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Thr Val Asn Arg Asn Asp
485 490 495
Leu Ile Pro Ser Asn Val Ile Asp Gln Thr Lys Ile Ala Ile Phe Gln
500 505 510
Asp Asn His Val Gly Val Asp Val Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asp Arg
530 535 540
Glu Arg Val Val Arg Ile Ser Thr Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Val Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn Asn Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Thr Asp Gly Phe Ser Phe Val Gly Val Ser Asn Ile Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Thr Met Ile Lys Leu
660 665
<210> 40
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 40
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Ile Arg Leu Val Ile Asp Pro Asn Arg Phe Leu Ser Val Ser Phe
20 25 30
Cys Asp Lys Pro Leu Leu Cys Cys Tyr Pro Asp Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asn Gln Ser Pro Leu Glu Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Phe Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Glu Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Asn Gln Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Ser Pro Glu Gln Gly Thr Met Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Asp Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asn Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Ser Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Ile Thr Ile Ser Glu Lys Asp Gly
385 390 395 400
Ser Lys Leu Gly Ile Gly Val Ala Met Asp Ser Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Glu Lys Leu Val Pro Ala
420 425 430
Gly Asn Tyr Ala Ile Ala Asn Gly Thr Gly Asn Asp Ile Thr Thr Ala
435 440 445
Lys Asp Tyr Asp Ser Ala Thr Val Ile Gln Asn Asn Thr Asn Phe Lys
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Thr Arg Ser Asp Asn Thr
485 490 495
Ile Thr Pro Ser Asn Val Ile Asp Pro Thr Lys Ile Ala Val Tyr Gln
500 505 510
Asp Thr His Val Gly Ala Glu Val Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Ile Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asp Arg
530 535 540
Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn Asn Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Thr Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Ala Ser Asn Val Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 41
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 41
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Gly Trp Asp Pro
1 5 10 15
His Phe Arg Leu Val Val Asn Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Asp Leu Leu Pro Glu Phe
35 40 45
Gly Thr Leu Trp Asp Cys Asp Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Ser Val Pro Pro Met His Trp Asp Ala Met Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Ile Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Ala Pro Glu Gln Gly Thr Val Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Thr Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Asp Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Tyr Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Ile Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Ser Asp Thr Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Ile Pro Ala Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Tyr Trp Ser Asp Ile Thr Glu Phe Val Val Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Val Thr Val Ser Glu Ser Gly Gly
385 390 395 400
Ser Lys Leu Gly Ile Gly Val Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Glu Lys Leu Thr Pro Ala
420 425 430
Gly Asn Tyr Ala Ile Thr Thr Ser Asn Asn Ser Asp Ile Ala Thr Ala
435 440 445
Thr Glu Tyr Asp His Ala Asp Glu Ile Lys Asn Asn Thr Asn Phe Lys
450 455 460
Ser Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Val Lys Glu Gly Asn Ser
485 490 495
Ile Thr Pro Ser Asn Thr Ile Asp Met Thr Lys Leu Val Val Tyr Gln
500 505 510
Asp Ala His Val Gly Asn Asp Val Gln Thr Ser Asp Val Thr Leu Ala
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu Gln Ala Ile Gly Ser Asp Arg
530 535 540
Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Thr Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Val Ser Ser Leu Pro Thr Leu Glu
625 630 635 640
Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 42
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 42
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Arg Leu Ile Ile Asn Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Glu Ile Phe Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Ser Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Ser Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Thr Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Thr His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Thr Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Tyr Trp Ser Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Ile Thr Ile Ser Val Lys Glu Ser
385 390 395 400
Ala Lys Leu Gly Thr Gly Val Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Glu Glu Leu Thr Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Thr Asn Ser Ser Gly Asn Asp Ile Thr Thr Ala
435 440 445
Ala Gly Phe Asp Ala Ala Asp Val Ile Lys Asn Asn Thr Asn Phe Arg
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Gly Thr Val Glu Gly Gly Lys
485 490 495
Ile Arg Pro Ser Asn Val Ile Asp Gln Thr Lys Ile Ala Val Phe Gln
500 505 510
Asp Thr His Ala Asn Arg Glu Val Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asp Arg
530 535 540
Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn His Tyr Leu Leu Ser Pro Asp Ser Leu Ala Val
595 600 605
Tyr Arg Ile Thr Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Val Ser Asn Ile Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Val Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 43
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 43
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Lys Leu Val Ile Asn Pro Asn Lys Phe Leu Pro Ile Gly Phe
20 25 30
Cys Asp Asn Pro Leu Leu Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Gly Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Glu Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met Asn Tyr Ile Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Ala Pro Glu Gln Gly Thr Val Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ser Gln Met Ser Thr Ala Ala Asp Met Ala Ser Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ser Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Ile Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Asn Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Arg Thr Ser Ser Leu Trp Val Gly Asn
340 345 350
Arg Phe Trp Gly Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Gln Phe Arg Pro Ile Thr Val Thr Ile Ser Val Lys Asn Gly
385 390 395 400
Glu Lys Leu Gly Ile Gly Ile Ala Thr Asp Phe Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Gly Glu Glu Leu Val Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Thr Thr Ser Ser Gly Asn Asp Ile Thr Thr Ala
435 440 445
Gln Gln Tyr Asp Ala Ala Asp Ile Ile Asn Asn Asn Thr Asn Phe Lys
450 455 460
Ser Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Thr Val Ser Gly Asn Lys
485 490 495
Leu Ile Pro Ser Asn Val Ile Asp Gln Thr Lys Ile Ala Ile Phe Gln
500 505 510
Asp Thr His Ala Asn Gln Gly Val Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Ile Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asn Arg
530 535 540
Glu Arg Val Val Arg Ile Ser Thr Leu Pro Glu Ala Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Thr Met Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn Asn Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Thr Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Thr Asp Gly Phe Ser Phe Val Gly Val Ser Ser Ile Gly Asn Leu Glu
625 630 635 640
Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Thr Met Thr Lys Leu
660 665
<210> 44
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 44
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Ile Gln Leu Val Ile Asn Pro Asn Lys Phe Leu Pro Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Phe Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Phe Glu Ala Val Glu
65 70 75 80
Pro Ser Val Pro Pro Met His Trp Asp Glu Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Met Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Thr His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Ile
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Asp Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Ser Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Ile Thr Ile Ser Val Asn Asn Ala
385 390 395 400
Ala Lys Leu Gly Thr Gly Val Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Ser Glu Leu Thr Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Thr Asn Ser Ser Gly Asn Asp Ile Thr Thr Ala
435 440 445
Val Gly Tyr Asp Thr Ala Asp Glu Ile Lys Asn Asn Thr Asn Phe Arg
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Gly Ile Val Thr Gly Asn Lys
485 490 495
Leu Gln Pro Ser Asn Thr Ile Asp Gln Thr Lys Ile Ala Val Phe Gln
500 505 510
Asp Thr His Ala Asn Lys Asp Pro Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asp Arg
530 535 540
Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Ala Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn His Tyr Leu Leu Ser Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Glu Gly Phe Ser Phe Val Gly Val Ser Ser Ile Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Thr Leu Thr Lys Leu
660 665
<210> 45
<211> 667
<212> PRT
<213> 猫杯状病毒
<400> 45
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Arg Leu Ile Ile Asn Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Glu Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Ser Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Ser Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Thr Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Thr His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Gly Val Asp
225 230 235 240
Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe Asp
245 250 255
Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu Arg Ser
260 265 270
Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val Ile
275 280 285
Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Thr Asn Ser
290 295 300
Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe Lys
305 310 315 320
Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser Val
325 330 335
Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn Arg
340 345 350
Tyr Trp Ser Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe Gln
355 360 365
Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser Thr
370 375 380
Pro Arg Phe Arg Pro Ile Thr Ile Thr Ile Ser Val Lys Glu Leu Ala
385 390 395 400
Lys Leu Gly Thr Gly Val Ala Thr Asp Tyr Ile Val Pro Gly Ile Pro
405 410 415
Asp Gly Trp Pro Asp Thr Thr Ile Pro Glu Glu Leu Thr Pro Ala Gly
420 425 430
Asp Tyr Ala Ile Thr Asn Ser Ser Gly Asn Asp Ile Thr Thr Ala Ala
435 440 445
Asp Phe Asp Ala Ala Asp Met Ile Lys Asn Asn Thr Asn Phe Arg Gly
450 455 460
Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys Ile
465 470 475 480
Ser Asn Thr Ala Phe Ile Thr Thr Gly Thr Val Glu Gly Gly Lys Ile
485 490 495
Arg Pro Ser Asn Val Ile Asp Gln Thr Lys Ile Ala Val Phe Gln Asp
500 505 510
Thr His Val Asn Gln Glu Val Gln Thr Ser Asp Asp Thr Leu Ala Leu
515 520 525
Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asp Arg Asp
530 535 540
Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly Gly
545 550 555 560
Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val Ile
565 570 575
Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg Gln
580 585 590
Leu Ser Leu Asn His Tyr Leu Leu Ser Pro Asp Ser Leu Ala Val Tyr
595 600 605
Arg Ile Thr Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp Ser
610 615 620
Asp Gly Phe Ser Phe Val Gly Val Ser Asn Ile Gly Lys Leu Glu Phe
625 630 635 640
Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Val Arg Leu
645 650 655
Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 46
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 46
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Lys Leu Val Ile Asn Pro Asn Lys Phe Leu Ala Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asn Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Glu Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Ser Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ala Ala Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Thr Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ala Ser Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Ile
225 230 235 240
Asn Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Ile Pro Asp Leu Arg
260 265 270
Asn Ser Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Thr Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Ile Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Tyr Trp Ser Asp Ile Thr Asp Phe Ile Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Val Thr Ile Ser Gln Lys Glu Gly
385 390 395 400
Glu Lys Leu Gly Ile Gly Val Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Gly Lys Leu Thr Pro Val
420 425 430
Gly Asn Tyr Ala Ile Thr Asp Glu Ser Gly Ser Asp Ile Val Thr Ala
435 440 445
Ala Glu Phe Asp Ser Ala Thr Gly Ile Lys Asn Asn Thr Glu Phe Arg
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Lys Val Asn Asn Asn Met
485 490 495
Leu Glu Pro Ser Asn Val Ile Asp Gln Thr Lys Ile Ala Ile Phe Gln
500 505 510
Asp Asn His Val Gly Arg Asp Val Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu Gln Ala Ile Gly Ser Asp Arg
530 535 540
Asp Arg Val Val Arg Ile Ser Ile Leu Pro Glu Ala Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Leu Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn His Tyr Leu Leu Ser Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Val Ser Ala Ile Gly Lys Leu Glu
625 630 635 640
Tyr Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Val Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 47
<211> 669
<212> PRT
<213> 猫杯状病毒
<400> 47
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Arg Leu Val Ile Asp Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asn Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ala Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Phe Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asn Gly Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Ile Leu Thr His His Leu Leu Ser Glu Val Ala Lys
100 105 110
Gly Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Gly Asp Ser Asp
115 120 125
Ser Ser Val Thr Thr Pro Glu Gln Gly Thr Met Val Gly Gly Val Ile
130 135 140
Ala Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Ser Gly
145 150 155 160
Lys Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser
165 170 175
Val Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln
180 185 190
Ser Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ala Lys Leu
195 200 205
Tyr Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly
210 215 220
Ser Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly
225 230 235 240
Val Asn Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu
245 250 255
Phe Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Ile Pro Asp Leu
260 265 270
Arg Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu
275 280 285
Val Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Thr
290 295 300
Asn Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp
305 310 315 320
Phe Arg Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly
325 330 335
Ser Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly
340 345 350
Asn Arg His Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val
355 360 365
Phe Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp
370 375 380
Ser Thr Pro Arg Phe Arg Pro Leu Thr Val Thr Ile Ser Gln Lys Asp
385 390 395 400
Gly Asp Lys Leu Gly Thr Gly Val Ala Thr Asp Tyr Ile Val Pro Gly
405 410 415
Ile Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Gly Glu Leu Ile Pro
420 425 430
Ala Gly Asp Tyr Ala Ile Thr Thr Gly Asn Gly Thr Asp Ile Thr Thr
435 440 445
Ala Gln Val Phe Asp Ser Ala Asp Val Ile Gln Asn Asn Thr Asn Phe
450 455 460
Arg Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys
465 470 475 480
Lys Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Ile Val Lys Gly Gly
485 490 495
Lys Leu Arg Pro Ser Asn Val Ile Asp Gln Thr Lys Ile Ala Ile Phe
500 505 510
Gln Asp Asn His Val Gln Ala Gly Ile Gln Thr Ser Asp Asp Thr Leu
515 520 525
Ala Val Leu Gly Tyr Thr Gly Ile Gly Glu Gln Ala Ile Gly Ala Asp
530 535 540
Arg Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg
545 550 555 560
Gly Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr
565 570 575
Val Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser
580 585 590
Arg Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser Phe Ala
595 600 605
Val Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile
610 615 620
Asp Ser Asp Gly Phe Ser Phe Val Gly Val Ser Asn Ile Gly Lys Leu
625 630 635 640
Glu Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile
645 650 655
Arg Leu Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 48
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 48
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Arg Leu Ile Ile Asn Pro Asn Lys Phe Leu Pro Ile Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Asp Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr His Glu Ala Ile Asp
65 70 75 80
Pro Ser Val Pro Pro Met His Trp Asp Ser Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Ala Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Ser Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ser Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Val Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Val Leu Thr His Gly Ser
325 330 335
Ile Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Tyr Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Ile Thr Ile Ser Glu Lys Asn Gly
385 390 395 400
Ser Lys Leu Gly Ile Gly Val Ala Thr Asp Tyr Ile Ile Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Ala Asp Lys Leu Ile Pro Ala
420 425 430
Gly Asp Tyr Ser Ile Thr Thr Gly Glu Gly Asn Asp Ile Lys Thr Ala
435 440 445
Gln Ala Tyr Asp Thr Ala Ala Val Val Lys Asn Thr Thr Asn Phe Arg
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Ile Arg Asp Gly Asn Glu
485 490 495
Ile Lys Pro Ser Asn Thr Ile Asp Met Thr Lys Leu Ala Val Tyr Gln
500 505 510
Asp Thr His Val Gly Gln Glu Val Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ser Asn Arg
530 535 540
Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Ala Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Glu Gly Phe Ser Phe Val Gly Val Ser Asp Ile Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Arg Met Thr Lys Leu
660 665
<210> 49
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 49
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asn Trp Asp Pro
1 5 10 15
His Ile Lys Leu Ser Ile Asn Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Met Trp Asp Cys Asp Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Glu Met Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Ile Ile Gly Glu Val Ala Lys
100 105 110
Gly Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Met Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Pro Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Gln His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Asp Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Pro Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Tyr Arg Pro Ile Thr Val Ser Ile Ser Gln Gln Glu Gly
385 390 395 400
Ala Lys Leu Gly Ile Gly Val Ala Ile Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Arg Ser Thr Ile Pro Ser Lys Leu Val Pro Ala
420 425 430
Gly Gly Tyr Ala Ile Thr Ala Lys Asn Gly Asn Asp Ile Thr Thr Ala
435 440 445
Ala Gln Tyr Asp Ala Ala Gly Glu Ile Val Asn Asn Thr Asn Phe Lys
450 455 460
Ser Met Tyr Ile Cys Gly Ala Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Val Ser Asn Thr Ala Phe Val Thr Thr Ala Thr Val Thr Gly Asn Thr
485 490 495
Leu Lys Pro Ser Asn Thr Ile Asp Asn Thr Lys Ile Ala Ile Tyr Gln
500 505 510
Asp Asn His Val Asn Ser Asp Val Gln Thr Ser Asp Val Thr Leu Ser
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Val Gly Ala Asp Arg
530 535 540
Asp Lys Leu Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn Asn Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ala Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Val Ser Asn Ile Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Thr Met Thr Lys Leu
660 665
<210> 50
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 50
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Gly Trp Asp Pro
1 5 10 15
His Phe Arg Leu Thr Ile Asn Pro Asn Asp Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asn Asp Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Gln Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Leu Gly Lys Val Ala Lys
100 105 110
Gly Trp Asp Pro Asn Leu Pro Ser Phe Arg Leu Glu Ser Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Leu Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Thr Gln Met Ala Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Thr Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Asn
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Ile Ala Gln Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Asp Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Gln Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Ile Pro Asp Leu Arg
260 265 270
Ser Asn Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Ile Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Val His Gly Ser
325 330 335
Ile Pro Ser Asn Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Ser Asp Ile Thr Asp Phe Val Ile Arg Pro Ser Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Lys Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Met Thr Ile Thr Ile Ser Gln Lys Gln Ser
385 390 395 400
Ala Lys Leu Gly Ile Ala Val Ala Leu Glu Ser Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Asp Ser Asn Leu Ile Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Thr Asn Gln Ala Asp Asn Asp Ile Thr Thr Ala
435 440 445
Met Glu Tyr Asp Ala Ala Thr Glu Ile Lys Asn Asn Thr Asn Phe Arg
450 455 460
Ser Met Tyr Ile Cys Gly Ala Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Val Ser Asn Thr Ala Phe Ile Thr Thr Ala Asp Leu Asp Gly Asn Thr
485 490 495
Ile Lys Pro Asn Asn Val Ile Asn Gln Ser Arg Ile Ile Val Tyr Gln
500 505 510
Asp Asn His Val Asn Leu Gln Val Gln Thr Ser Glu Val Thr Leu Ala
515 520 525
Met Leu Gly Tyr Thr Gly Ile Gly Glu Glu Val Ile Gly Ala Asn Arg
530 535 540
Asp Arg Val Val Arg Ile Asn Val Leu Pro Glu Val Ser Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Tyr Tyr Lys Asn Ser Leu Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ala Leu Asn Asn Tyr Leu Leu Asp Pro Asp Cys Phe Ala Val
595 600 605
Tyr Arg Ile Thr Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Tyr Ser Gly Phe Ser Phe Val Gly Val Ser Asn Ile Gly Ala Leu Glu
625 630 635 640
Phe Pro Leu Thr Ala Ser Tyr Thr Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 51
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 51
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Gly Trp Asp Pro
1 5 10 15
His Phe Asp Leu Val Ile Asn Pro Asn Asp Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asn Asp Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Lys Gln Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly His Leu Met His His Leu Ile Gly Lys Val Ser Lys
100 105 110
Gly Trp Asp Pro Asn Leu Pro Asn Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Ala Pro Glu Gln Gly Thr Leu Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Thr Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Gly His Leu Ala Gln Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Asp Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Arg Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Ile Pro Asp Leu Arg
260 265 270
Ser Ser Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Gly Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Ile Thr Val Glu Thr Lys Pro Gly Ala Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Val Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Thr Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Ala Asp Ile Thr Asp Phe Val Ile Arg Pro Ser Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Lys Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Met Thr Val Thr Ile Ser Gln Ser Gly Gly
385 390 395 400
Ala Lys Leu Gly Ile Gly Ile Ala His Glu Thr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Gly Glu Leu Val Pro Ala
420 425 430
Gly Glu Tyr Ala Ile Val Asn Gln Thr Ser Asn Asp Ile Ala Thr Ala
435 440 445
Val Asp Tyr Asp Thr Ala Thr Ala Ile Thr Asn Asn Thr Asn Phe Lys
450 455 460
Ser Met Tyr Ile Cys Gly Ala Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Asp Leu Asn Gly Asn Lys
485 490 495
Ile Lys Pro Asn Asn Val Ile Asn Gln Gln Arg Ile Val Ile Phe Gln
500 505 510
Asp Asn His Val Gly Ser Glu Ala Gln Thr Ser Asp Val Thr Leu Ala
515 520 525
Met Leu Gly Tyr Thr Gly Ile Gly Glu Glu Val Ile Gly Ala Asp Arg
530 535 540
Asp Arg Val Val Arg Ile Asn Val Leu Pro Glu Val Ser Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Thr Leu Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ala Leu Asn Asn Tyr Leu Leu Asp Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Thr Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Tyr Ser Gly Phe Ser Phe Val Gly Val Ser Asn Ile Gly Glu Leu Glu
625 630 635 640
Phe Pro Leu Thr Ala Ser Tyr Met Gly Val Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 52
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 52
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asn Trp Asp Pro
1 5 10 15
His Phe Lys Met Val Val Asn Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Lys Pro Leu Leu Cys Cys Tyr Pro Asp Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asn Gln Ser Pro Leu Glu Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Phe Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Asp Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Ser Glu Val Ala Lys
100 105 110
Gly Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Ala Pro Glu Gln Gly Thr Val Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Pro Gln Met Ala Val Ala Ala Asp Thr Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ser Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Leu Pro Asp Leu Arg
260 265 270
Asn Ser Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Val Val Thr Val Glu Thr Lys Pro Gly Ala Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Arg Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Tyr Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Ile Thr Ile Ser Glu Lys Asn Gly
385 390 395 400
Glu Lys Leu Gly Ile Gly Val Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Ala Glu Lys Leu Thr Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Thr Thr Gly Asp Gly Asn Asp Ile Ile Thr Ala
435 440 445
Asn Ala Tyr Asp Thr Ala Asp Val Val Lys Asn Asn Thr Asn Phe Arg
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Gly Thr Val Ser Asp Asn Lys
485 490 495
Ile Lys Pro Ser Asn Val Ile Asp Pro Thr Lys Leu Ala Val Phe Gln
500 505 510
Asp Asn His Val Gly Ala Asp Ala Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu His Ala Ile Gly Ala Asp Arg
530 535 540
Glu Arg Val Val Arg Ile Ser Thr Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Val Thr Asn Ile Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Ile Lys Leu
660 665
<210> 53
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 53
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asn Trp Asp Pro
1 5 10 15
His Phe Lys Leu Val Ile Asn Pro Asn Lys Phe Leu Ser Ile Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asn Glu Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Ile Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Ala Pro Glu Gln Gly Thr Val Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ser Gln Met Ser Thr Ala Ala Asp Met Ala Ser Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ser Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Ile Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Ser Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Thr Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Phe Trp Ser Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Val Thr Ile Ser Glu Lys Asn Gly
385 390 395 400
Ala Lys Leu Gly Val Gly Val Ala Thr Asp Phe Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Gly Glu Lys Leu Val Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Thr Asn Gly Ser Gly Asn Asp Ile Thr Thr Ala
435 440 445
Asn Gln Tyr Asp Ala Ala Asp Ile Ile Arg Asn Asn Thr Asn Phe Lys
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Thr Val Glu Gly Asn Asp
485 490 495
Leu Ile Pro Ser Asn Val Ile Asp Gln Thr Lys Ile Ala Ile Phe Gln
500 505 510
Asp Asn His Val Gln Asp Glu Val Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asn Arg
530 535 540
Glu Arg Val Val Arg Ile Ser Thr Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Val Gly Ile Asp
610 615 620
Phe Asp Gly Phe Ser Phe Val Gly Val Ser Asp Val Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Thr Met Thr Lys Leu
660 665
<210> 54
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 54
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Ile Arg Leu Val Ile Asp Pro Asn Arg Phe Leu Ser Val Ser Phe
20 25 30
Cys Asp Lys Pro Leu Leu Cys Cys Tyr Pro Asp Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asn Gln Ser Pro Leu Glu Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr Phe Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Glu Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Asn Gln Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Ser Pro Glu Gln Gly Thr Met Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Asp Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asn Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Ser Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Ile Thr Ile Ser Glu Lys Asp Gly
385 390 395 400
Ser Lys Leu Gly Ile Gly Val Ala Met Asp Ser Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Glu Lys Leu Val Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Ala Asn Gly Thr Gly Asn Asp Ile Thr Thr Ala
435 440 445
Lys Asp Tyr Asp Ser Ala Thr Val Ile Gln Asn Asn Thr Asn Phe Lys
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Thr Arg Ser Asp Asn Thr
485 490 495
Ile Thr Pro Ser Asn Val Ile Asp Pro Thr Lys Ile Ala Val Tyr Gln
500 505 510
Asp Thr His Val Gly Ala Glu Val Gln Thr Ser Asp Asp Thr Leu Ala
515 520 525
Ile Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asp Arg
530 535 540
Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn Asn Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Thr Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Ala Ser Asn Val Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Thr Lys Leu
660 665
<210> 55
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 55
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Arg Leu Asn Val Asn Pro Asn Glu Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Lys Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Ser Arg Ala Pro Leu Glu Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ser Ser Thr His Glu Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Glu Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Asn Lys Val Ala Arg
100 105 110
Ala Trp Asp Pro Asn Leu Pro Leu Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Leu Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ser Gln Met Ser Ala Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ser Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Val Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Asp Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Asn Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Leu Ser Val Thr Ile Ser Gln Ser Gly Gly
385 390 395 400
Ala Lys Leu Gly Ile Gly Ile Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Gly Glu Leu Thr Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Val Asn Gln Asn Asn Ser Asp Ile Ala Thr Lys
435 440 445
Gln Ala Tyr Glu Ser Ala Thr Ser Ile Glu Asn Asn Thr Asn Phe Lys
450 455 460
Ser Met Tyr Ile Cys Gly Ser Leu His Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Thr Val Asp Gly Asn Asn
485 490 495
Leu Ile Pro Ser Asn Thr Ile Gly Gln Asp Lys Ile Ala Val Phe Gln
500 505 510
Asp Asn His Val Asn Ala Glu Val Gln Thr Ser Asp Val Thr Leu Ala
515 520 525
Thr Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Ile Arg
530 535 540
Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ala Met Lys Leu Gly Tyr Val
565 570 575
Ile Gly Asp Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn Asn Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Val Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Val Ser Ser Ile Pro Lys Leu Glu
625 630 635 640
Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Thr Met Thr Lys Leu
660 665
<210> 56
<211> 671
<212> PRT
<213> 猫杯状病毒
<400> 56
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Gly Trp Asp Pro
1 5 10 15
His Phe Lys Leu Val Ile Asn Pro Asn Asn Phe Leu Pro Val Gly Phe
20 25 30
Cys Ser Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Arg Ser Pro Leu Glu Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Phe Asp Ala Val Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Ser Ala Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His His Leu Ile Gly Lys Val Ala Ala
100 105 110
Gly Trp Asp Pro Asp Leu Pro Leu Ile Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Ala Pro Glu Gln Gly Thr Met Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ser Thr Ala Ala Asp Met Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ala Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Thr Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Tyr Trp Ser Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Ser Val Thr Ile Ser Gln Gln Asp Gly
385 390 395 400
Ala Lys Leu Gly Ile Gly Val Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Gly Glu Leu Ile Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Thr Asn Asn Ile Gly Asp Asp Ile Thr Thr Ala
435 440 445
Thr Gly Tyr Asp Thr Ala Asp Ile Ile Lys Asn Asn Thr Asn Phe Lys
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Ala Thr Leu Ser Gly Asn Asn
485 490 495
Asn Asn Lys Ile Asn Pro Cys Asn Thr Ile Asp Gln Ser Lys Ile Val
500 505 510
Val Phe Gln Asp Asn His Val Gly Lys Glu Val Gln Thr Ser Asp Asp
515 520 525
Thr Leu Ala Leu Leu Gly Tyr Thr Gly Ile Gly Glu Gln Ala Ile Gly
530 535 540
Ser Asp Arg Asp Arg Val Val Arg Ile Ser Thr Leu Pro Glu Thr Gly
545 550 555 560
Ala Arg Gly Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu
565 570 575
Gly Tyr Val Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His
580 585 590
Thr Ser Arg Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser
595 600 605
Phe Ala Val Tyr Arg Ile Ile Asp Ser Asn Gly Ser Trp Phe Asp Ile
610 615 620
Gly Ile Asp Ser Asp Gly Phe Ser Phe Val Gly Val Ser Gly Phe Gly
625 630 635 640
Lys Leu Glu Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala
645 650 655
Lys Ile Arg Leu Ala Ser Asn Ile Arg Ser Pro Met Thr Lys Leu
660 665 670
<210> 57
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 57
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Ile Arg Leu Val Ile Asn Pro Asn Lys Phe Leu Pro Ile Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Tyr Asp Ala Ile Asp
65 70 75 80
Pro Val Val Pro Pro Met His Trp Asp Asp Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His Phe Leu Ile Asn Glu Val Ala Lys
100 105 110
Gly Trp Asp Pro Ser Leu Pro Ser Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Ala Val Gly Gly Val Ile Ala
130 135 140
Gln Pro Ser Ala Gln Met Ser Ala Ala Ala Asp Met Ala Ser Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Glu Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Thr Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Glu His Leu Ser Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Asp Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Val
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Ser Leu Pro Asp Leu Arg
260 265 270
Asn Ser Leu Tyr His Leu Ile Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Glu Ala Asn
290 295 300
Ser Ser Gly Cys Ile Val Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Ile Pro Ser Asp Leu Ile Pro Arg Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg His Trp Thr Asp Ile Thr Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Ile Asn Ile Ser Gln Lys Glu Gly
385 390 395 400
Ala Arg Leu Gly Thr Ala Ile Ala Val Asp Thr Ile Leu Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Thr Val Leu Thr Pro Ala
420 425 430
Gly Lys Tyr Ala Ile Thr Ser Gly Asn Asn Ser Asp Ile Leu Thr Arg
435 440 445
Asn Asp Tyr Glu Asn Ala Asp Val Ile Lys Asn Asn Thr Asn Phe Arg
450 455 460
Ser Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Asp
465 470 475 480
Ile Ser Asn Thr Ala Phe Ile Thr Thr Gly Thr Val Asn Gly Asn Asn
485 490 495
Ile Glu Pro Ser Asn Thr Ile Asp Pro Thr Lys Leu Ala Val Phe Gln
500 505 510
Asp Asn His Val Lys Ser Glu Val Gln Thr Ser Asp Val Thr Leu Ser
515 520 525
Val Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Val Gly Ala Asp Arg
530 535 540
Asp Lys Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Met Lys Leu Gly Tyr Val
565 570 575
Ile Ser Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn His Tyr Leu Leu Pro Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Thr Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Asn Asp Gly Phe Ser Phe Val Gly Val Ser His Ile Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Ser Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Ser Met Ile Lys Leu
660 665
<210> 58
<211> 668
<212> PRT
<213> 猫杯状病毒
<400> 58
Met Cys Ser Thr Cys Ala Asn Val Leu Lys Tyr Tyr Asp Trp Asp Pro
1 5 10 15
His Phe Arg Leu Val Ile Asp Pro Asn Lys Phe Leu Ser Val Gly Phe
20 25 30
Cys Asp Asn Pro Leu Met Cys Cys Tyr Pro Glu Leu Leu Pro Glu Phe
35 40 45
Gly Thr Val Trp Asp Cys Asp Gln Ser Pro Leu Gln Ile Tyr Leu Glu
50 55 60
Ser Ile Leu Gly Asp Asp Glu Trp Ala Ser Thr Tyr Glu Ala Ile Asp
65 70 75 80
Pro Ser Val Pro Pro Met His Trp Asp Ser Ala Gly Lys Ile Phe Gln
85 90 95
Pro His Pro Gly Val Leu Met His Tyr Ile Ile Gly Glu Val Ala Lys
100 105 110
Ala Trp Asp Pro Asn Leu Pro Asn Phe Arg Leu Glu Ala Asp Asp Gly
115 120 125
Ser Ile Thr Thr Pro Glu Gln Gly Thr Met Val Gly Gly Val Ile Ala
130 135 140
Glu Pro Ser Ala Gln Met Ala Thr Ala Ala Asp Thr Ala Thr Gly Lys
145 150 155 160
Ser Val Asp Ser Glu Trp Glu Ala Phe Phe Ser Phe His Thr Ser Val
165 170 175
Asn Trp Ser Thr Ser Glu Thr Gln Gly Lys Ile Leu Phe Lys Gln Ser
180 185 190
Leu Gly Pro Leu Leu Asn Pro Tyr Leu Thr His Leu Ala Lys Leu Tyr
195 200 205
Val Ala Trp Ser Gly Ser Ile Glu Val Arg Phe Ser Ile Ser Gly Ser
210 215 220
Gly Val Phe Gly Gly Lys Leu Ala Ala Ile Val Val Pro Pro Gly Ile
225 230 235 240
Asp Pro Val Gln Ser Thr Ser Met Leu Gln Tyr Pro His Val Leu Phe
245 250 255
Asp Ala Arg Gln Val Glu Pro Val Ile Phe Thr Ile Pro Asp Leu Arg
260 265 270
Ser Thr Leu Tyr His Leu Met Ser Asp Thr Asp Thr Thr Ser Leu Val
275 280 285
Ile Met Val Tyr Asn Asp Leu Ile Asn Pro Tyr Ala Asn Asp Ser Asn
290 295 300
Ser Ser Gly Cys Ile Leu Thr Val Glu Thr Lys Pro Gly Pro Asp Phe
305 310 315 320
Lys Phe His Leu Leu Lys Pro Pro Gly Ser Met Leu Thr His Gly Ser
325 330 335
Val Pro Ser Asp Leu Ile Pro Lys Ser Ser Ser Leu Trp Ile Gly Asn
340 345 350
Arg Tyr Trp Ser Asp Ile Asn Asp Phe Val Ile Arg Pro Phe Val Phe
355 360 365
Gln Ala Asn Arg His Phe Asp Phe Asn Gln Glu Thr Ala Gly Trp Ser
370 375 380
Thr Pro Arg Phe Arg Pro Ile Thr Val Thr Ile Ser Val Lys Glu Ser
385 390 395 400
Ala Lys Leu Gly Ile Gly Val Ala Thr Asp Tyr Ile Val Pro Gly Ile
405 410 415
Pro Asp Gly Trp Pro Asp Thr Thr Ile Pro Thr Asp Leu Thr Pro Ala
420 425 430
Gly Asp Tyr Ala Ile Thr Asp Ser Thr Gly Asn Asp Ile Thr Thr Pro
435 440 445
Ser Gly Tyr Asp Ser Ala Ile Ser Ile Val Asn Asn Thr Asn Phe Arg
450 455 460
Gly Met Tyr Ile Cys Gly Ser Leu Gln Arg Ala Trp Gly Asp Lys Lys
465 470 475 480
Val Ser Asn Thr Ala Phe Ile Thr Thr Gly Ile Val Thr Gly Asn Lys
485 490 495
Leu Glu Pro Ser Asn Thr Ile Asp Gln Thr Lys Ile Ala Ile Phe Gln
500 505 510
Asp Thr His Ala Asn Arg Asp Thr Gln Ile Ser Asp Asp Thr Leu Ala
515 520 525
Leu Leu Gly Tyr Thr Gly Ile Gly Glu Glu Ala Ile Gly Ala Asp Arg
530 535 540
Asp Arg Val Val Arg Ile Ser Val Leu Pro Glu Thr Gly Ala Arg Gly
545 550 555 560
Gly Asn His Pro Ile Phe Tyr Lys Asn Ser Ile Lys Leu Gly Tyr Val
565 570 575
Ile Arg Ser Ile Asp Val Phe Asn Ser Gln Ile Leu His Thr Ser Arg
580 585 590
Gln Leu Ser Leu Asn His Tyr Leu Leu Ser Pro Asp Ser Phe Ala Val
595 600 605
Tyr Arg Ile Thr Asp Ser Asn Gly Ser Trp Phe Asp Ile Gly Ile Asp
610 615 620
Ser Asp Gly Phe Ser Phe Val Gly Val Asn Asn Ile Gly Lys Leu Glu
625 630 635 640
Phe Pro Leu Thr Ala Ser Tyr Met Gly Ile Gln Leu Ala Lys Ile Arg
645 650 655
Leu Ala Ser Asn Ile Arg Ser Thr Met Thr Lys Leu
660 665
<210> 59
<211> 7685
<212> DNA
<213> 猫杯状病毒
<400> 59
gtaaaagaaa tttgagacaa tgtctcaaac tctgagcttc gtgcttaaaa ctcacagcgt 60
ccgcaaggac tttgtgcact ccgtcaagct aacgcttgca cggaggcgcg atcttcagta 120
tttctataac aagctctctc gttctatgcg agctgaagct tgtccttctt gtgccagtta 180
tgatgtatgt cctaactgca cttctagtga tatccctgat gatggttcat caacaacgtt 240
gatgccatca tgggaggatg tcacaaaaac ctctacttac tctcttctac tctccgaaga 300
tacttctgac gagttgtctc ctgacgaatt ggttcacgtt gctgctcaca ttcgcaaggc 360
gctttccact cagtctcatc cggccaatgt tgacatgtgc aaagaacagc tcacgtcatt 420
attggtgatg gctgaagcta tgctgcctca acggtcacga gcaacaatcc cacttcatca 480
acaacaccag acggctcgca ttgaatggag ggaaaagttc ttctctaagc cccttaactt 540
cattcttgaa aagattggtg tttctaaaga tattcttcag gttactgccc tttggaaaat 600
catcttggag aaagcttgct actgcaaatc ctatggtgaa gagtggttcg atgctgcaaa 660
gaacaaacta agggagatga gaagcttcga aagcaacaca ctcaaaccac tggttggagc 720
tttcattgac ggcttaaggt ttatgactgt ggataacccc aaccctatgg cattcttacc 780
caagctaatt gggcttatca aacccctcaa tttggcaatg ataatcgaca atcatgaaaa 840
cacactatca ggatggatca ttaccctcac tgcaattatg gagttataca acatcacaga 900
atgcacaatt gacataatca cttcactggt aacagcattc tatgacaaaa ttgctaaagc 960
ccctaaattc tacagtcaga tcaaagcact gttcacggga tttaggacag aggatgtggc 1020
taattcattt tggtatatgg ctgctgcaat tctatgttac ctgattacgg gtataatccc 1080
aaacaatggg aggttttcaa agattaaaac ctgcctgtct ggtgcaacga ccctggtatc 1140
gggtataatt gccacacaga aacttgcagc aatgttcgct acatggaact ctgaatccat 1200
tgttaacgaa ttatctgcaa ggacagtggc tatttctgaa ttgaataacc caactactac 1260
ctctgacact gactctgttg agcggttgct cgaattggct aagatcttgc acgaggagat 1320
caaagttcac actctgaatc caattatgca atcatacaac ccaattctta gaaatttaat 1380
gtccacacta gacggtgttg tcacctcatg caacaagaga agagcaattg ccaaaaagag 1440
gcaagtccca gtttgctaca tccttactgg accacctgga tgtggcaaga ccactgcggc 1500
cctagccctt gcaaagaagc tctctgatca agagccatca gtgattaatt tggatgttga 1560
tcatcatgat acttacactg gcaatgaagt gtgcattgtt gatgagtttg attcatcaga 1620
caaagtggac tatgcaaatt ttgtgattgg tatggttaat tcggcaccaa tggttctaaa 1680
ttgtgatatg cttgaaaata agggaaagct ctttacctct aagtacataa taatgacttc 1740
taactctgaa acccctgtaa aaccctcttc taagcgtgca ggtgctttct accgaagagt 1800
tacaatcatt gacgttgcaa accctttggt ggaatctcac aagcgcgcgc gccctggaac 1860
atctgtccct cgaagctgct acaagaagaa cttttcacac ctttcacttg ctaaacgagg 1920
ggctgagtgc tggtgcaagg agtatgtttt ggaccccaag ggacttcagc atcagagtat 1980
caaggctcct cctcccactt ttcttaacat tgattccctt gcccaaacta tgaaacaaga 2040
cttcattctt aagaacatgg catttgaggc agagaatggc tctagtgagc acaggtttgg 2100
ctttgtctgt cagcagagtg aggtcgacac tgttcgcagg ctgcttaatg caataagggc 2160
acggcttaac gcgacattca ctgtgtgcgt tgggcctgag tcctcaaatt ccattgggtg 2220
tactgctcat gtcctcactc ccgatgagcc tttcaatggg aagaggtttg ttgtatctcg 2280
atgcaacgaa gcctcccttg cagcactaga aggaaattgc gtgcagactg cgttgggcgt 2340
atgcatgtcc aacaaggatt taacccacct gtgccatttc ataagaggga agattgtcaa 2400
tgatagtgtc aggctggacg aactacccgc caatcaacat gtggtaaccg tgaattcggt 2460
atttgatttg gcctgggctc ttcgccgtca cctcacacta tctggacagt ttcaagcaat 2520
cagagccgca tatgatgtgc taactgtccc cgacaaaatt ccagctatgt taaggcactg 2580
gatggatgaa acatcctttt cggatgagca tgtagtcaca cagtttgtaa cgccaggtgg 2640
tattgtcatc ttggaatcat gtggtggagc acgtatctgg gctttgggtc acaatgtgat 2700
ccgcgctgga ggtatcactg caacaccaac tggtggttgt gtcagattaa tgggtctctc 2760
tgccccttct atgccatggt ctgaactctt tcgagaactt ttctctttat tgggtaggat 2820
atggtctagt gttaaagtct ccactcttgt tctgaccgct cttggaatgt acgcatctag 2880
atttagaccc aaatcagaag ctaagggaaa aacaaaatcc aaggttgggc cctacagagg 2940
tcgtggtgta gcgctcactg atgatgagta cgatgaatgg agggaacaca acgccacacg 3000
aaaattggac ttatcagtag aagattttct gatgctgcgc catcgtgcag cacttggtgc 3060
tgatgatgca gatgcagtta aatttagatc atggtggaac tcaagatcaa aattggcgga 3120
tgattttgaa gatgtcaccg tgattggaaa aggtggtgtt aaacatgaaa gaatcagaac 3180
aaacaatctc agagctgtgg agcgtggata tgacgttagc tttgctgaag aatcaggacc 3240
cggcacaaaa ttccacaaaa acgccattgg atctgtaact gatgtttgtg gtgagcacaa 3300
gggatactgt gtccatatgg gtcatggggt gtacgcctca gtagcccatg tggtgaaagg 3360
tgattcattc tttcttggtg agagaatatt tgacctcaaa actaatggtg agttttgctg 3420
ctttcgcagt acaaagatct tgcccagtgc tgcgcctttc ttctcaggca aacccacccg 3480
tgacccgtgg ggctcacctg ttgccacaga atggaagcca aaaccataca ccactacatc 3540
agggaagatt atagggtgtt ttgctacaac ttcaactgaa acccacccag gtgattgtgg 3600
tctaccttac atcgatgaca atggtagagt cactggactg cacacaggat ccggtggacc 3660
caaaactccc agcgcaaaat tggttgtccc ctatgtccat attgatatga aaacaaaatc 3720
tgttactgcc caaaagtatg acattacaaa gcctgatatt agttacaaag gattgatttg 3780
caaacaattg gatgaaattc ggataatacc aaaaggaaca cgcctccacg tttctcctgc 3840
ccacacagat gattatgagg aatgttcgca ccaaccagca tcactaggta gtggtgatcc 3900
acgatgtcca aaatccctta ctgctattgt agttgactcc ttaaaaccct actgtgataa 3960
ggttgagggc cccccccatg atgttttgca tagggttcag aaaatgctaa ttgatcacct 4020
ctctggcttt gttcctatga atatctcttc tgaaaactca atgctctctg catttcacaa 4080
actcaatcat gatacatcct gtggcccata cttaggtgga agaaagaaag atcatatggt 4140
aaatggtgag cctgacaagg cactgttgga tttgctatct tcaaaatgga aacttgccac 4200
tcaagggatt gctctccctc atgagtacac aattgggcta aaagatgaat tgagaccaat 4260
tgagaaagtt caggaaggaa agagaaggat gatctggggt tgtgatgttg gggttgccac 4320
agtttgcgct gctgcattta aaggtgttag tgacgcaata acggcaaacc accagtatgg 4380
acctgttcaa gttgggatta acatggatag ccccagtgtt gaggcacttt accaacgaat 4440
caagagcgct gccaaggttt ttgcggtcga ttactccaag tgggattcga ctcaatcgcc 4500
tcgggttagt gctgcctcaa ttgatattct tagatacttc tctgatagat ctccaattgt 4560
tgattcagct gcaaacacct taaaaagtcc tccaatagcg gtgttcaatg gtgttgcagt 4620
aaaggtttcg tctggtcttc catctggtat gcctctgact tctgtaatta actctttaaa 4680
tcactgccta tatgttggtt gtgctatcct tcaatctctg gaatccaaaa acatacctgt 4740
cacttggaat ctgttctcct catttgacat gatgacttac ggtgatgatg gtgtttacat 4800
gttccctatc atgtttgcta gtgttagtga tcaaatcttt ggaaacctta ctgcttatgg 4860
cttaaaaccc acaagggtcg acaaatcagt tggagctatt gaaccaatcg atcctgaatc 4920
tgttgttttc ctaaaaagaa ccatccaaag gacccctagt ggcattcgag gtcttcttga 4980
tcgcagctca atcctcagac agttctatta cattaaaggt gaaaattcgg atgattggaa 5040
aactcctcca aaagtcatag atcccacctc cagaggtcaa cagctgtgga acgcttgtct 5100
ttatgctagc caacatggtg ttgagtttta caataaggtt ctcaaattgg cccagaaagc 5160
tgttgaatac gaaggattac acctcgaccc cccaagcttc caatcggcat tggaacatta 5220
caataaccaa tttagtggtg tggaggcgcg gaatgaccag atcgattcga gtggcatgtc 5280
cgccctacac tgtgatgtgt tcgaagtttg agcatgtgct caacctgcgc taacgtgctt 5340
aaatactatg attgggatcc ccacttcaaa ttggtgatcg accctaataa atttctttct 5400
gttggattct gtgataaccc gcttatgtgt tgctatcctg agttgcttcc agaattcggc 5460
actgtgtggg attgtgatca atctccactg caaatttacc ttgaatctat tcttggtgat 5520
gatgaatggt cttctaccta tgaagccatt gaccctgtcg ttccaccaat gcactgggac 5580
aaaatgggga aaatcttcca acctcaccct ggtatgctaa tgcaccatat catcggtgaa 5640
gttgccaagg gatgggatcc aaacttgcct ttgttccgat tggaggccga tgatggatcg 5700
gtgacaaccc ctgaacaagg aacaatggtt ggcggagtca ttgctgaacc cagcgcccaa 5760
atgtcaactg ctgctgacat ggcctctggg aaaagtgttg actccgaatg ggaggctttc 5820
ttctcatttc acactagtgt aaattggagt acatctgaaa cacaaggaaa gattctattc 5880
aaacaatcac ttggacccct gctaaaccca tatcttgaac acatatctaa gctttatgtt 5940
gcatggtctg gatctataga ggttaggttt tctatttctg gttctggtgt ttttggggga 6000
aaactcgctg ctattgttgt gccgcctggg gtagacccag tgcaaagcac atcaatgcta 6060
cagtatcctc atgttctatt tgatgcccgt caggtagaac ctgttatctt ttcaatacct 6120
gatttgagaa acagcttgta ccatcttatg tcagatactg atactacctc ccttgtaatt 6180
atggtgtaca atgatcttat taatccttat gcaaatgatt ctaactcttc tggatgtatt 6240
gtcactgttg agactaaacc tggaatcgat ttcaagtttc accttctgaa accccctgga 6300
tctatgctta cccatggctc tatcccttca gatttaatac caaaatcttc ttctctttgg 6360
atagggaatc gtcactggac tgacataacc gattttgtca ttaggccttt tgtgttccaa 6420
gcaaaccgtc attttgattt taatcaggaa acagctggtt ggagtacgcc aagattccga 6480
ccactagccg taacggttag ccaaagtaaa ggagcaaaat tggggaatgg tattgcgacc 6540
gactacatcg tgcctgggat tcctgatgga tggccagaca ctacaatccc aacaaaactg 6600
acacccacag gcgattatgc aattacatct agtgatggta atgacattga aacaaaactt 6660
gaatacgaaa atgctgatgt gattaaaaac aacacaaact tcaggagcat gtacatttgt 6720
gggtcgctcc aaagagcctg gggtgataag aagatttcta atactggttt cataactact 6780
ggtgttatct ctgataattc cataagccca agcaatacca ttgatcaaag caaaatcgtg 6840
gtctatcaag acaatcatgt caatagtgaa gttcaaacct cagacatcac acttgcaatt 6900
cttggctaca ctggtattgg tgaggaggca attggcgcca atcgggactc ggttgtacgc 6960
ataagtgtgc tgccagaaac aggtgcccgt gggggaaacc atccaatctt ttataagaat 7020
tctatgaagc ttggttacgt gatcagctcc attgatgtgt ttaattccca gatcttgcac 7080
acctctagac aactatcact gaataactac ttactacctc cagattcttt tgctgtctat 7140
cgaattattg attctaatgg gtcttggttt gatataggca ttgattctga tggattttct 7200
tttgttggtg tctcaagttt tcctaagtta gagtttccac tttctgcctc ctatatggga 7260
attcagttgg caaagattcg tcttgcctct aatattagga gttctatgac taaattatga 7320
attcaatatt aggcttaatt gacactgtta caaatacaat tggcaaagct caacaaattg 7380
aattagataa agctgcactt ggtcaacaac gcgaactggc tttgcaacga attaaccttg 7440
atcgccaggc gttgaacaac caagttgagc aatttaacaa aattcttgag caaagggtac 7500
aaggccccat ccagtctgtg cggctcgcac gtgctgctgg atttagggtt gacccttact 7560
catacacaaa tcaaaatttt tacgatgacc aattgaatgc aattagacta tcttatagga 7620
atttgtttaa ataattggcc acaagtatcc cttcgggctg ccgcatttgc gcctaacccc 7680
agggc 7685
<210> 60
<211> 7690
<212> DNA
<213> 猫杯状病毒
<400> 60
gtaaaagaaa tttgagacaa tgtctcaaac tctgagcttc gtgcttaaaa ctcacagtgt 60
ccgaaaggac tttgtgcact ctgtcaagtt aacacttgca cggaggcgcg atcttcagta 120
tatttataac aagctctcac gcactatacg tgctgaggct tgcccttctt gtgctagtta 180
cgacgtatgt cctaactgca cctctggtga cgtcccggat gacgggtctt cgacaatgtc 240
gattccatca tgggaggatg tcacaaagtc ttcaacctac tctctcctgc tctctgagga 300
cacctccgac gagttatgcc ctgaggactt ggttaatgtg gctgctcata tccgtaaggc 360
gctatccact cagtcccacc cggctaatgc tgaaatgtgc aaagaacagc tcactttctt 420
attagtcatg gctgaggcga tgctgcccca acgatcccga gcgtcaatcc cactgcacca 480
acaacacacg gctgcacggt tggaatggag ggagaaattc ttctctaaac ctcttgactt 540
tctccttgaa agagttggtg tgtcaaaaga tattctccaa actactgcga tttggaaaat 600
tatcttggaa aaagcatgct attgtaaatc ctatggagag cagtggttca ctgctgccaa 660
acaaaagtta agggaaatga aaaactttga gagtgatacg ctaaaacctc ttattggtgg 720
atttatagat ggtctacggt tcttgaccgt ggacaaccca aacccgatgg gtttcctccc 780
aaaactcata gggcttgtaa aacccctgaa tttggcgatg attattgata atcatgaaaa 840
cacaatatct ggctggatca tcacattaac cgcaataatg gagctataca acatcaccga 900
atgcaccata gatattataa catcagtcat tacggctttc tatgataaaa ttggcaaggc 960
aaccaaattt tacagttgtg ttaaggcgct gttcactgga tttagatctg aggacgtagc 1020
gaattccttt tggtacatgg cagcagcgat tctatgttac ctgatcactg ggttaatccc 1080
aaacaacggc agattttcaa agataaaagc ttgcctggcc ggagcgacaa ctcttgtatc 1140
aggtatagtt gccacacaaa agctagctgc aatgttcgca acatggaact ctgagtccat 1200
tgttaatgag ttgtcagcaa gaactgttgc cctatcagag ctaaacaatc caacaacaac 1260
gtctgacacg gattcggtag aacgactgct agaattggct aagatcttgc atgaggagat 1320
caagattcat actctaaacc ccatcatgca atcatacaat ccaatcttga gaaatttaat 1380
gtcaaccttg gacggtgtta taacatcatg caacaagagg aaagctattg ctcggaagag 1440
acaggtgcca gtttgttaca tattaactgg cccacctgga tgtgggaaaa caaccgcagc 1500
tcaagcatta gctaagaaat tgtctgatca agagccgtcg gtaattaacc ttgatgttga 1560
tcatcatgac acatatactg gaaatgaggt atgtatcatt gatgagtttg attcgtctga 1620
caaggtagac tatgcaaatt ttgtgattgg gatggtaaac tctgctccta tggtgttaaa 1680
ttgtgacatg cttgagaaca agggaaagct cttcacctca aagtatataa ttatgacttc 1740
caactctgaa actccagtga aaccctcttc caagcgcgca ggtgcattct accgaagggt 1800
tactatcatc gatgtaacta acccttttgt ggagtcgcac aagcgtgcaa ggcctgggac 1860
gtctgttcct cgtagctgct ataagaaaaa cttctcccat ctctcacttg ctaaacgagg 1920
ggccgagtgc tggtgcaagg aatacgttct tgaccctaag gggcttcaac accaaagcat 1980
gaaggctccc cctcctacct ttcttaacat tgactcttta gctcagacga tgaagcaaga 2040
cttcttgctc aagaacatgg cttttgaggc tgaagatggg tgcgcagaac atcgatatgg 2100
gtttgtgtgt caacaggaag aggttgaaac tgttcgcagg ctcctcaacg cggttagggc 2160
aaggatgaat gctaccttca ctgtgtgtgt tggacccgag acctcgcact cgattggttg 2220
cactgcgcac gtgttaactc ccaatgagac ctttaatgga aagaagtttg ttgtgtcacg 2280
atgtaacgaa gcatcacttt ctgcccttga aggtaactgt gtaaagtcag ctttgggcgt 2340
gtgtatgtca gataaggatc tcactcattt atgccacttc attaaaggga aaattgtcaa 2400
tgacagtgtt aggttggatg aactacccgc caatcagcat gtggtaaccg ttaattcggt 2460
gtttgatttg gcctgggctg ttcgtcgtca tctcacactg gcagggcagt ttcaagctat 2520
cagagccgca tatgatgtgc ttactgtccc cgacaagatc cctgcaatgt tgcgtcactg 2580
gatggatgag acctcctttt ctgatgacca cgttgtaaca caatttgtaa cacctggtgg 2640
catagtcatc ctggagtctt gcggtggtgc acgcatctgg gctttaggtc gcaacgtgat 2700
ccgagcagga ggtgtcaccg caaccccaac cggaggatgt gttaggttaa tgggattatc 2760
tgcgccaacc atgccatggt ccgagatctt tcgtgagctc ttctctctct taggtagaat 2820
ttggtcatct gtcaaagtat cagccctagt actaactgct cttggaatgt acgcatctag 2880
atttagacca aaatcggaag caaaaggaaa aaccaaattg aagattggga catacagggg 2940
tcgcggtgta gcgctgactg atgacgagta cgatgagtgg cgcgaacaca acgcctccag 3000
aaaattggat ttgtcagtgg aggatttctt aatgttgcgc catcgtgccg ctctaggagc 3060
cgacgacaat gatgcagtaa aattccggtc gtggtggaac tccagaacca aaatggccaa 3120
tgattatgag gatgtcaccg taattggcaa aggtggcgtc aaacatgaaa agatcagaac 3180
caatacccta aaagctgtgg atcgtggtta tgacgtcagc tttgctgagg aatcaggacc 3240
aggcaccaag ttccacaaga atgcaattgg atctgtcaca gatgtttgtg gggagcacaa 3300
aggctattgc atccacatgg gccacggtgt ttacgcatcc gtagctcacg tggtgaaagg 3360
ggattcattt ttcttgggtg aaaggatttt tgatcttaag actaatggtg aattttgctg 3420
ctttcgcagc acgaaaattc tacctagtgc tgcacctttc ttttctggga agcccactcg 3480
tgatccgtgg ggatcccccg tggcaactga gtggaagcct aaaatgtaca caacaacctc 3540
tggaaagatt ctggggtgct ttgcaacaac ttcaactgaa actcacccgg gagactgtgg 3600
cctcccatat attgatgaca acgggagggt gaccggcctc cacactggct ctgggggacc 3660
caaaacccca agtgccaagt tggtggtgcc atatgtgcat attgacatga agactaaatc 3720
cgtcactgct caaaagtatg acgtaacaaa gcctgatata agttacaaag gcttaatttg 3780
taagcaattg gatgagatta ggattatacc aaaaggcaca cgtctccatg tctccccagc 3840
ccacactgag gattatcaag aatgctcaca ccaacccgca tcacttggaa gcggggatcc 3900
ccgctgtcca aaatctctca ctgctatagt tgttgattct ctaaaaccat actgtgagaa 3960
cgttgagggt cctccacatg atgttttgca cagagttcaa aagatgctta tcgaccacct 4020
ttcaggcttt gtccctatga acatttcctc ggaaacctct atgctctcag ctttccacaa 4080
actcaatcat gatacttcct gtggaccata cttgggtggc agaaagaaag atcacatggc 4140
taacggtgag ccggacaagc agttattgga tctcctgtct gcaaaatgga aattggcaac 4200
ccaaggcata gcactaccac atgagtacac aattgggcta aaggacgagt taaggcccgt 4260
ggagaaggtt agtgaaggga agagaaggat gatttggggt tgtgatgttg gcgtcgctac 4320
tgtctgtgca gctgcgttca agggtgttag cgatgccatc acagcaaacc accagtacgg 4380
gcctatacag gttggtatca acatggatag ccccagcgtc gaagcgctgt tccaaaggat 4440
caaaagcgcg gccaaggtat ttgcggtcga ttattccaaa tgggattcga cccaatcgcc 4500
tcgtgtcagt gcagcttcaa ttgacatcct tcgttacttt tccgatcgct ctccaattgt 4560
tgactcagcc tctaacacac tgaagagccc tcctgttgca atctttaatg gtgttgctgt 4620
aaaagtgtcc tctggcttac catctggaat gcctcttacc tcagtaatca attcccttaa 4680
tcattgtctg tatgttgggt gtgccattct tcaatcccta gaagctaagg ccattcccgt 4740
cacttggaac cttttctcaa cttttgatat catgacttac ggggatgatg gtgtctacat 4800
gtttcctatt atgtatgcaa gtattagtga ccaaattttt ggaaatcttt cttcctatgg 4860
cctgaaacca actcgggttg acaagtccgt tggagcaatt gagcctattg atcctgactc 4920
tgttgttttc ttgaagagaa caatcacaag gacacctcag gggataaggg gtttacttga 4980
tcgcagctct ataataagac aattctatta tattaaaggt gagaactccg atgactggaa 5040
gagcccccca aaacatattg acccaacatc tcgagggcaa cagctttgga atgcctgtct 5100
gtacgctagc caacatggct tggagttttt caacaaggtt tacaggctgg ccgagagggc 5160
tgttgaatat gaagagctgc actttgaacc cccaacatat gcttcggctt tggatcatta 5220
caacagccag ttcaatggcg tggaggcgcg gtctgaccag atcgactcga gtggcatgac 5280
cgccctacac tgtgatgtgt tcgaagtttg agcatgtgct caacctgcgc taacgtgctt 5340
aaatattatg attgggaccc ccatttcaaa ttggtaatca accccaacaa cttcctctct 5400
gttggctttt gtagtaaccc tttaatgtgt tgctacccag aactccttcc ggaatttgga 5460
actgtttggg attgcgatcg gtcaccactt gaaatttacc tagaatcaat acttggtgat 5520
gatgaatggg catccacttt tgacgctgtt gacccagtcg ttcccccaat gcactggggt 5580
gctgctggaa aaattttcca gccacacccc ggtgttctca tgcaccatct cattggtaag 5640
gttgctgcag gttgggaccc cgatctgcct ctaattcgac tcgaggcgga tgacgggtca 5700
atcacagcac ccgagcaagg aacaatggtt ggcggcgtca tcgctgaacc cagcgcccag 5760
atgtcaacag ctgctgatat ggccaccggg aaaagcgttg attctgagtg ggaggcattc 5820
ttctcctttc acaccagcgt caattggagt acatctgaaa cccaaggaaa gattctcttc 5880
aaacaatcct taggcccttt gctcaaccca tatctagaac accttgctaa gctatatgtt 5940
gcgtggtctg ggtcgattga ggttaggttc tctatctctg gctctggtgt ctttggtggg 6000
aagctcgcag ctattgttgt acctcctggg gttgatccag tgcagagtac ttcgatgcta 6060
caataccccc atgttttgtt tgatgctcgt caggtggaac cagttatctt ctgtcttcct 6120
gatctaagaa gcaccctgta ccaccttatg tctgacactg acactacatc cttggtcatt 6180
atggtgtaca atgatctcat caatccctat gccaatgatg ccaactcttc tgggtgtatt 6240
gtcactgtcg agacaaaacc tggccctgac ttcaagtttc acctccttaa gccacccgga 6300
tctatgctaa cccatggctc tatcccttct gatttaattc ccaaaacatc ttcgctctgg 6360
atcggtaacc gctactggtc agacataact gattttgtga ttcggccgtt tgtcttccaa 6420
gcaaatcgtc attttgactt taatcaagag accgcagggt ggagcacacc acggtttcgg 6480
cctatatctg ttaccattac tgaacagaac ggagcaaaat tgggcattgg ggtggcaaca 6540
gattacatag tgcctggaat ccctgatggc tggcctgaca ccacaattcc tggggagttg 6600
ataccagctg gtgattacgc aatcaccaat ggtactggca atgacatcac cacggctaca 6660
ggatatgaca ctgctgatat aattaagaac aataccaact ttaggggcat gtacatatgt 6720
ggttcgctcc agcgtgcctg gggtgataag aaaatttcca acactgcctt tatcaccact 6780
gccaccctag atggtgacaa caacaacaag atcaatccct gtaataccat agaccagtca 6840
aagatcgtcg tgtttcaaga caaccatgtt ggaaagaaag cgcaaacctc agacgataca 6900
ttggccctgc ttggttacac tggcattggt gagcaggcca tcgggtctga tagggaccgg 6960
gttgtgcgca tcagcactct ccctgaaact ggtgctcgag gcggtaacca cccaattttc 7020
tacaagaact ccattaaatt gggatatgta attaggtcta ttgatgtctt taattcacaa 7080
atcttgcaca cttccagaca gttatcgcta aatcattacc tactcccacc tgattctttt 7140
gccgtctata gaataattga ctcaaatggc tcgtggtttg atattggaat tgatagtgat 7200
gggttctctt ttgttggtgt ttctggcttt ggtaaattag aatttcccct ttctgcctcc 7260
tacatgggaa tacaattggc aaagatccgg cttgcctcta acattaggag tcccatgact 7320
aagttatgaa ttcaatatta ggcctgattg atactgttac taacactatt ggtaaagctc 7380
agcagattga attggacaaa gctgcacttg gtcaacagcg tgaattggct ctccagcgca 7440
ttggcttgga ccgccaagcc ttaaacaacc aagttgagca gtttaacaaa attcttgagc 7500
aaagggtaca aggcccaatc caatccgttc gccttgcgcg cgcggctgga tttagggttg 7560
acccttactc atacacaaac caaaactttt atgacgatca attaaatgca attagactat 7620
catataaaaa tttgtttaaa atttgatcat atatcccttt gggctgccgc acctgcgcct 7680
aaccccaggg 7690
<210> 61
<211> 7690
<212> DNA
<213> 猫杯状病毒
<400> 61
gtaaaagaaa tttgagacaa tgtctcaaac tctgagcttc gtgcttaaaa ctcacagcgt 60
ccgcaaggac tttgtgcact ccgtcaagct aacgcttgca cggaggcgcg atcttcagta 120
tttctataac aagctctctc gttctatgcg agctgaagct tgtccttctt gtgccagtta 180
tgatgtatgt cctaactgca cttctagtga tatccctgat gatggttcat caacaacgtt 240
gatgccatca tgggaggatg tcacaaaaac ctctacttac tctcttctac tctccgaaga 300
tacttctgac gagttgtctc ctgacgaatt ggttcacgtt gctgctcaca ttcgcaaggc 360
gctttccact cagtctcatc cggccaatgt tgacatgtgc aaagaacagc tcacgtcatt 420
attggtgatg gctgaagcta tgctgcctca acggtcacga gcaacaatcc cacttcatca 480
acaacaccag acggctcgca ttgaatggag ggaaaagttc ttctctaagc cccttaactt 540
cattcttgaa aagattggtg tttctaaaga tattcttcag gttactgccc tttggaaaat 600
catcttggag aaagcttgct actgcaaatc ctatggtgaa gagtggttcg atgctgcaaa 660
gaacaaacta agggagatga gaagcttcga aagcaacaca ctcaaaccac tggttggagc 720
tttcattgac ggcttaaggt ttatgactgt ggataacccc aaccctatgg cattcttacc 780
caagctaatt gggcttatca aacccctcaa tttggcaatg ataatcgaca atcatgaaaa 840
cacactatca ggatggatca ttaccctcac tgcaattatg gagttataca acatcacaga 900
atgcacaatt gacataatca cttcactggt aacagcattc tatgacaaaa ttgctaaagc 960
ccctaaattc tacagtcaga tcaaagcact gttcacggga tttaggacag aggatgtggc 1020
taattcattt tggtatatgg ctgctgcaat tctatgttac ctgattacgg gtataatccc 1080
aaacaatggg aggttttcaa agattaaaac ctgcctgtct ggtgcaacga ccctggtatc 1140
gggtataatt gccacacaga aacttgcagc aatgttcgct acatggaact ctgaatccat 1200
tgttaacgaa ttatctgcaa ggacagtggc tatttctgaa ttgaataacc caactactac 1260
ctctgacact gactctgttg agcggttgct cgaattggct aagatcttgc acgaggagat 1320
caaagttcac actctgaatc caattatgca atcatacaac ccaattctta gaaatttaat 1380
gtccacacta gacggtgttg tcacctcatg caacaagaga agagcaattg ccaaaaagag 1440
gcaagtccca gtttgctaca tccttactgg accacctgga tgtggcaaga ccactgcggc 1500
cctagccctt gcaaagaagc tctctgatca agagccatca gtgattaatt tggatgttga 1560
tcatcatgat acttacactg gcaatgaagt gtgcattgtt gatgagtttg attcatcaga 1620
caaagtggac tatgcaaatt ttgtgattgg tatggttaat tcggcaccaa tggttctaaa 1680
ttgtgatatg cttgaaaata agggaaagct ctttacctct aagtacataa taatgacttc 1740
taactctgaa acccctgtaa aaccctcttc taagcgtgca ggtgctttct accgaagagt 1800
tacaatcatt gacgttgcaa accctttggt ggaatctcac aagcgcgcgc gccctggaac 1860
atctgtccct cgaagctgct acaagaagaa cttttcacac ctttcacttg ctaaacgagg 1920
ggctgagtgc tggtgcaagg agtatgtttt ggaccccaag ggacttcagc atcagagtat 1980
caaggctcct cctcccactt ttcttaacat tgattccctt gcccaaacta tgaaacaaga 2040
cttcattctt aagaacatgg catttgaggc agagaatggc tctagtgagc acaggtttgg 2100
ctttgtctgt cagcagagtg aggtcgacac tgttcgcagg ctgcttaatg caataagggc 2160
acggcttaac gcgacattca ctgtgtgcgt tgggcctgag tcctcaaatt ccattgggtg 2220
tactgctcat gtcctcactc ccgatgagcc tttcaatggg aagaggtttg ttgtatctcg 2280
atgcaacgaa gcctcccttg cagcactaga aggaaattgc gtgcagactg cgttgggcgt 2340
atgcatgtcc aacaaggatt taacccacct gtgccatttc ataagaggga agattgtcaa 2400
tgatagtgtc aggctggacg aactacccgc caatcaacat gtggtaaccg tgaattcggt 2460
atttgatttg gcctgggctc ttcgccgtca cctcacacta tctggacagt ttcaagcaat 2520
cagagccgca tatgatgtgc taactgtccc cgacaaaatt ccagctatgt taaggcactg 2580
gatggatgaa acatcctttt cggatgagca tgtagtcaca cagtttgtaa cgccaggtgg 2640
tattgtcatc ttggaatcat gtggtggagc acgtatctgg gctttgggtc acaatgtgat 2700
ccgcgctgga ggtatcactg caacaccaac tggtggttgt gtcagattaa tgggtctctc 2760
tgccccttct atgccatggt ctgaactctt tcgagaactt ttctctttat tgggtaggat 2820
atggtctagt gttaaagtct ccactcttgt tctgaccgct cttggaatgt acgcatctag 2880
atttagaccc aaatcagaag ctaagggaaa aacaaaatcc aaggttgggc cctacagagg 2940
tcgtggtgta gcgctcactg atgatgagta cgatgaatgg agggaacaca acgccacacg 3000
aaaattggac ttatcagtag aagattttct gatgctgcgc catcgtgcag cacttggtgc 3060
tgatgatgca gatgcagtta aatttagatc atggtggaac tcaagatcaa aattggcgga 3120
tgattttgaa gatgtcaccg tgattggaaa aggtggtgtt aaacatgaaa gaatcagaac 3180
aaacaatctc agagctgtgg agcgtggata tgacgttagc tttgctgaag aatcaggacc 3240
cggcacaaaa ttccacaaaa acgccattgg atctgtaact gatgtttgtg gtgagcacaa 3300
gggatactgt gtccatatgg gtcatggggt gtacgcctca gtagcccatg tggtgaaagg 3360
tgattcattc tttcttggtg agagaatatt tgacctcaaa actaatggtg agttttgctg 3420
ctttcgcagt acaaagatct tgcccagtgc tgcgcctttc ttctcaggca aacccacccg 3480
tgacccgtgg ggctcacctg ttgccacaga atggaagcca aaaccataca ccactacatc 3540
agggaagatt atagggtgtt ttgctacaac ttcaactgaa acccacccag gtgattgtgg 3600
tctaccttac atcgatgaca atggtagagt cactggactg cacacaggat ccggtggacc 3660
caaaactccc agcgcaaaat tggttgtccc ctatgtccat attgatatga aaacaaaatc 3720
tgttactgcc caaaagtatg acattacaaa gcctgatatt agttacaaag gattgatttg 3780
caaacaattg gatgaaattc ggataatacc aaaaggaaca cgcctccacg tttctcctgc 3840
ccacacagat gattatgagg aatgttcgca ccaaccagca tcactaggta gtggtgatcc 3900
acgatgtcca aaatccctta ctgctattgt agttgactcc ttaaaaccct actgtgataa 3960
ggttgagggc cccccccatg atgttttgca tagggttcag aaaatgctaa ttgatcacct 4020
ctctggcttt gttcctatga atatctcttc tgaaaactca atgctctctg catttcacaa 4080
actcaatcat gatacatcct gtggcccata cttaggtgga agaaagaaag atcatatggt 4140
aaatggtgag cctgacaagg cactgttgga tttgctatct tcaaaatgga aacttgccac 4200
tcaagggatt gctctccctc atgagtacac aattgggcta aaagatgaat tgagaccaat 4260
tgagaaagtt caggaaggaa agagaaggat gatctggggt tgtgatgttg gggttgccac 4320
agtttgcgct gctgcattta aaggtgttag tgacgcaata acggcaaacc accagtatgg 4380
acctgttcaa gttgggatta acatggatag ccccagtgtt gaggcacttt accaacgaat 4440
caagagcgct gccaaggttt ttgcggtcga ttactccaag tgggattcga ctcaatcgcc 4500
tcgggttagt gctgcctcaa ttgatattct tagatacttc tctgatagat ctccaattgt 4560
tgattcagct gcaaacacct taaaaagtcc tccaatagcg gtgttcaatg gtgttgcagt 4620
aaaggtttcg tctggtcttc catctggtat gcctctgact tctgtaatta actctttaaa 4680
tcactgccta tatgttggtt gtgctatcct tcaatctctg gaatccaaaa acatacctgt 4740
cacttggaat ctgttctcct catttgacat gatgacttac ggtgatgatg gtgtttacat 4800
gttccctatc atgtttgcta gtgttagtga tcaaatcttt ggaaacctta ctgcttatgg 4860
cttaaaaccc acaagggtcg acaaatcagt tggagctatt gaaccaatcg atcctgaatc 4920
tgttgttttc ctaaaaagaa ccatccaaag gacccctagt ggcattcgag gtcttcttga 4980
tcgcagctca atcctcagac agttctatta cattaaaggt gaaaattcgg atgattggaa 5040
aactcctcca aaagtcatag atcccacctc cagaggtcaa cagctgtgga acgcttgtct 5100
ttatgctagc caacatggtg ttgagtttta caataaggtt ctcaaattgg cccagaaagc 5160
tgttgaatac gaaggattac acctcgaccc cccaagcttc caatcggcat tggaacatta 5220
caataaccaa tttagtggtg tggaggcgcg gaatgaccag atcgattcga gtggcatgac 5280
cgccctacac tgtgatgtgt tcgaagtttg agcatgtgct caacctgcgc taacgtgctt 5340
aaatattatg attgggaccc ccatttcaaa ttggtaatca accccaacaa cttcctctct 5400
gttggctttt gtagtaaccc tttaatgtgt tgctacccag aactccttcc ggaatttgga 5460
actgtttggg attgcgatcg gtcaccactt gaaatttacc tagaatcaat acttggtgat 5520
gatgaatggg catccacttt tgacgctgtt gacccagtcg ttcccccaat gcactggggt 5580
gctgctggaa aaattttcca gccacacccc ggtgttctca tgcaccatct cattggtaag 5640
gttgctgcag gttgggaccc cgatctgcct ctaattcgac tcgaggcgga tgacgggtca 5700
atcacagcac ccgagcaagg aacaatggtt ggcggcgtca tcgctgaacc cagcgcccag 5760
atgtcaacag ctgctgatat ggccaccggg aaaagcgttg attctgagtg ggaggcattc 5820
ttctcctttc acaccagcgt caattggagt acatctgaaa cccaaggaaa gattctcttc 5880
aaacaatcct taggcccttt gctcaaccca tatctagaac accttgctaa gctatatgtt 5940
gcgtggtctg ggtcgattga ggttaggttc tctatctctg gctctggtgt ctttggtggg 6000
aagctcgcag ctattgttgt acctcctggg gttgatccag tgcagagtac ttcgatgcta 6060
caataccccc atgtcttgtt tgatgctcgt caggtggaac cagttatctt ctctattcct 6120
gatctaagaa gcaccctgta ccaccttatg tctgacactg acactacatc cttggtcatt 6180
atggtgtaca atgatctcat caatccctat gccaatgatg ccaactcttc tgggtgtatt 6240
gtcactgtcg agacaaaacc tggccctgac ttcaagtttc acctccttaa gccacccgga 6300
tctatgctaa cccatggttc tgtcccttct gatttaattc ccaaaacatc ttcgctctgg 6360
atcggtaacc gctactggtc agacataact gattttgtga ttcggccgtt tgtcttccaa 6420
gcaaatcgtc attttgactt taatcaagag accgcagggt ggagcacacc acggtttcgg 6480
cctatatctg ttaccattac tgaacagaac ggagcaaaat tgggcattgg agtggcaaca 6540
gattacatag tgcctggaat ccctgatggc tggcctgaca ccacaattcc tggggagttg 6600
ataccagctg gcgattacgc aatcaccaat ggtactggca atgacatcac cacggctaca 6660
ggatatgaca ctgctgatat aattaagaac aataccaact ttaggggcat gtacatatgt 6720
ggttcgctcc agcgtgcctg gggtgataag aaaatttcca acactgcctt tatcaccact 6780
gccaccctag atggtgacaa caacaacaag atcaatccct gtaataccat agaccagtca 6840
aagatcgtcg tgtttcaaga caaccatgtt ggaaagaaag tgcaaacctc agacgataca 6900
ttggccctgc ttggttacac tggcattggt gagcaggcca tcgggtctga tagggaccgg 6960
gttgtgcgca tcagcactct ccctgaaact ggtgctcgag gcggtaacca cccaattttc 7020
tacaagaact ccattaaatt gggatatgta attaggtcta ttgatgtctt taattcacaa 7080
atcttgcaca cttccagaca gttatcgcta aatcattacc tactcccacc tgattctttt 7140
gccgtctata gaataattga ctcaaatggc tcgtggtttg atattggaat tgatagtgat 7200
gggttctctt ttgttggtgt ttctggcttt ggtaaattag aatttcccct ttctgcctcc 7260
tacatgggaa tacaattggc aaagatccgg cttgcctcta acattaggag tcccatgact 7320
aagttatgaa ttcaatatta ggcctgattg atactgttac taacactatt ggtaaagctc 7380
aacagattga attggacaaa gctgcacttg gtcaacagcg tgaattggct ctccagcgca 7440
ttggcttgga ccgccaagcc ttaaacaacc aagttgagca gtttaacaaa attcttgagc 7500
aaagggtaca aggcccaatc caatccgttc gccttgcacg cgcggctgga tttagggttg 7560
acccttactc atacacaaac caaaactttt atgacgatca attaaatgca attagactat 7620
catataaaaa tttgtttaaa atttgatcat atatcccttt gggctgccgc acctgcgcct 7680
aaccccaggg 7690
<210> 62
<211> 7684
<212> DNA
<213> 猫杯状病毒
<220>
<221> misc_feature
<222> (5829)..(5829)
<223> n是a, c, g,或t
<400> 62
gtaaaagaaa tttgagacaa tgtctcaaac tctgagcttc gtgcttaaaa ctcacagtgt 60
ccgaaaggac tttgtgcact ctgtcaagtt aacacttgca cggaggcgcg atcttcagta 120
tatttataac aagctctcac gcactatacg tgctgaggct tgcccttctt gtgctagtta 180
cgacgtatgt cctaactgca cctctggtga cgtcccggat gacgggtctt cgacaatgtc 240
gattccatca tgggaggatg tcacaaagtc ttcaacctac tctctcctgc tctctgagga 300
cacctccgac gagttatgcc ctgaggactt ggttaatgtg gctgctcata tccgtaaggc 360
gctatccact cagtcccacc cggccaatgc tgaaatgtgc aaagaacagc tcacttcctt 420
attagtcatg gctgaggcga tgctgcccca acgatcccga gcgtcaatcc cactgcacca 480
acaacacacg gctgcacggt tggaatggag ggagaaattc ttctctaaac ctcttgactt 540
tctccttgaa agagttggtg tgtcaaaaga tattctccaa actactgcga tttggaaaat 600
tatcttggaa aaagcatgct attgtaaatc ctatggagag cagtggttca ctgctgccaa 660
gcaaaagtta agggaaatga aaaactttga gagtgatacg ctaaaacctc ttattggtgg 720
atttatagat ggtctacggt tcttgaccgt ggacaaccca aacccgatgg gtttcctccc 780
aaaactcata gggcttgtaa aacccctgaa tttggcgatg attattgata atcatgaaaa 840
cacaatatct ggctggatca tcacattaac cgcaataatg gagctataca acatcaccga 900
atgcaccata gatattataa catcagtcat tacggctttc tatgataaaa ttggcaaggc 960
aaccaaattt tacagttgtg ttaaggcgct gttcactgga tttagatctg aggacgtagc 1020
gaattccttt tggtacatgg cagcagcgat tctatgttac ctgatcactg ggttaatccc 1080
aaacaatggc agattttcaa agataaaagc ttgcctggcc ggagcgacaa ctcttgtatc 1140
aggtatagtt gccacacaaa agctagctgc aatgttcgca acatggaact ctgagtccat 1200
tgttaatgag ttgtcagcaa gaactgttgc cctatcagag ctaaacaatc caacaacaac 1260
gtctgacacg gattcggtag aacgactgct agaattggct aagatcttgc atgaggagat 1320
caagattcat actctaaacc ccatcatgca atcatacaat ccaatcttga gaaatttaat 1380
gtcaaccttg gacggtgtta taacatcatg caacaagagg aaagctattg ctcggaagag 1440
acaggtgcca gtttgttaca tattaactgg cccacctgga tgtgggaaga caaccgcagc 1500
tcaagcatta gctaagaaat tgtctgatca agagccgtcg gtaattaacc ttgatgttga 1560
tcatcatgac acatatactg gaaatgaggt atgtatcatt gatgagtttg attcgtctga 1620
caaggtagac tatgcaaatt ttgtgattgg gatggtaaac tctgctccta tggtgttaaa 1680
ttgtgacatg cttgagaaca agggaaagct cttcacctca aagtatataa ttatgacttc 1740
caactctgaa actccagtga aaccctcttc caagcgcgca ggtgcattct accgaagggt 1800
tactatcatc gatgtaacta accctttggt ggagtcgcac aagcgtgcaa ggcctgggac 1860
gtctgttcct cgtagctgct ataagaaaaa cttctcccat ctctcacttg ctaaacgagg 1920
ggccgagtgc tggtgcaagg aatacgttct tgaccctaag gggcttcaac accaaagcat 1980
gaaggctccc cctcctacct ttcttaacat tgactcttta gctcagacga tgaagcaaga 2040
cttcttgctc aagaacatgg cttttgaggc tgaagatggg tgcgcagaac atcgatatgg 2100
gtttgtgtgt caacaggaag aggttgaaac tgttcgcagg ctcctcaacg cggttagggc 2160
aaggatgaat gctaccttca ctgtgtgtgt tggacccgag acctcgcact cgattggttg 2220
cactgcgcac gtgttaactc ccaatgagac ctttaatgga aagaagtttg ttgtgtcacg 2280
atgtaacgaa gcatcacttt ctgcccttga aggtaactgt gtaaagtcag ctttgggcgt 2340
gtgtatgtca gataaggatc tcactcattt atgccacttc attaaaggga aaattgtcaa 2400
tgacagtgtt aggttggatg aactacccgc caatcagcat gtggtaaccg ttaattcggt 2460
gtttgatttg gcctgggctc ttcgtcgtca tctcacactg gcagggcagt ttcaagctat 2520
cagagccgca tatgatgtgc ttactgtccc cgacaagatc cctgcaatgt tgcgtcactg 2580
gatggatgag acctcctttt ctgatgacca cgttgtgaca caatttgtaa cacctggtgg 2640
catagtcatc ctggagtctt gcggtggtgc acgcatctgg gctttaggtc acaacgtgat 2700
ccgagcagga ggtgtcaccg caaccccaac cggaggatgt gttaggttaa tgggattgtc 2760
tgccccaacc atgccatggt ccgagatctt tcgtgagctc ttctctctct taggtagaat 2820
ttggtcatct gtcaaagtat cagccctagt actaactgct cttggaatgt acgcatctag 2880
atttagacca aaatcggaag caaaaggaaa aaccaaattg aagattggaa catacagggg 2940
tcgcggtgta gcgctgactg atgacgagta cgatgagtgg cgcgaacaca acgcctccag 3000
aaaattggat ttgtcagtgg aggatttctt aatgttgcgc catcgtgccg ctctaggagc 3060
cgacgacaat gatgcagtaa aattccggtc gtggtggaac tccagaacca aaatggccaa 3120
tgattatgag gatgtcaccg taattggcaa aggtggcgtc aaacatgaaa agatcagaac 3180
caatacccta aaagctgtgg atcgtggtta tgacgtcagc tttgctgagg aatcaggacc 3240
aggcaccaag ttccacaaga atgcaattgg atctgtcaca gatgtttgtg gggagcacaa 3300
aggctattgc atccacatgg gccacggtgt ttacgcatcc gtagctcacg tggtgaaagg 3360
ggattcattt ttcttgggtg aaaggatttt tgatcttaag actaatggtg aattttgctg 3420
ctttcgcagc acgaagattc tacctagtgc tgcacctttc ttttctggga agcccactcg 3480
tgatccgtgg ggatcccccg tggcaactga gtggaagcct aaaatgtaca caacaacctc 3540
tggaaagatt ctggggtgct ttgcaacaac ttcaactgaa actcacccgg gagactgtgg 3600
cctcccatat attgatgaca acgggagggt gaccggcctc cacactggct ctgggggacc 3660
caaaacccca agtgccaagt tggtggtgcc atatgtgcat attgacatga agactaaatc 3720
cgtcactgct caaaagtatg acgtaacaaa gcctgatata agttacaaag gcttaatttg 3780
taagcaattg gatgagatta ggattatacc aaaaggcaca cgtctccatg tctccccagc 3840
ccacactgag gattatcaag aatgctcaca ccaacccgca tcacttggaa gcggggatcc 3900
ccgctgtcca aaatctctca ctgctatagt tgttgattct ctaaaaccat actgtgagaa 3960
ggttgagggt cctccacatg atgttttgca cagagttcaa aagatgctta tcgaccacct 4020
ttcaggcttt gtccctatga acatttcctc ggaaacctct atgctctcag ctttccacaa 4080
actcaatcat gatacttcct gtggaccata cttgggtggc agaaagaaag atcacatggc 4140
taacggtgag ccggacaagc agttattgga tctcctgtct gcaaaatgga aattggccac 4200
ccaaggcata gcactaccac atgagtacac aattgggcta aaggacgagt taaggcccgt 4260
ggagaaggtt agtgaaggga agagaaggat gatttggggt tgtgatgttg gcgtcgctac 4320
tgtctgtgca gctgcgttca agggtgttag cgatgccatc acagcaaacc accagtacgg 4380
gcctatacag gttggtatca acatggatag ccccagcgtc gaagcgctgt tccaaaggat 4440
caaaagcgcg gccaaggtat ttgcggtcga ttattccaaa tgggattcga cccaatcgcc 4500
tcgtgtcagt gcagcttcaa ttgacatcct tcgttacttc tctgatcgct ctccaattgt 4560
tgactcagcc tctaacacac tgaagagccc tcctgttgca atctttaatg gtgttgctgt 4620
aaaagtgtcc tctggcttac catctggaat gcctcttacc tcagtaatca attcccttaa 4680
tcattgtctg tatgttgggt gtgccattct tcaatcccta gaagctaagg ccattcccgt 4740
cacttggaac cttttctcaa cttttgatat catgacttac ggggatgatg gtgtctacat 4800
gtttcctatt atgtatgcaa gtattagtga ccaaattttt ggaaatcttt cgtcctatga 4860
cctgaaacca actcgggttg acaagtccgt tggagcaatt gagcctattg atcctgactc 4920
tgttgttttc ttgaagagaa caatcacaag gacacctcag gggataaggg gtttacttga 4980
tcgcagctct ataataagac aattctatta tattaaaggt gagaactccg atgactggaa 5040
gagcccccca aaacatattg acccaacatc tcgagggcaa cagctttgga atgcctgtct 5100
gtacgctagc caacatggct tggagttttt caacaaggtt tacaggctgg ccgagagggc 5160
tgttgaatat gaagagctgc actttgaacc cccaacatat gcttcggctt tggatcatta 5220
caacagccag ttcaatggcg tggaggcgcg gtctgaccag atcgactcga gtggcatgac 5280
cgccctacac tgtgatgtgt tcgaagtttg agcatgtgct caacctgcgc taacgtgctt 5340
aaatactatg attgggatcc ccacttcaaa ttggtgatcg accctaataa atttctttct 5400
gttggattct gtgataaccc gcttatgtgt tgctatcctg agttgcttcc agaattcggc 5460
actgtgtggg attgtgatca atctccactg caaatttacc ttgaatctat tcttggtgat 5520
gatgaatggt cttctaccta tgaagccatt gaccctgtcg ttccaccaat gcactgggac 5580
aaaatgggga aaatcttcca acctcaccct ggtatgctaa tgcaccatat catcggtgaa 5640
gttgccaagg gatgggatcc aaacttgcct ttgttccgat tggaggccga tgatggatcg 5700
gtgacaaccc ctgaacaagg aacaatggtt ggcggagtca ttgctgaacc cagcgcccaa 5760
atgtcaactg ctgctgacat ggcctctggg aaaagtgttg actccgaatg ggaggctttc 5820
ttctcattnc acactagtgt aaattggagt acatctgaaa cacaaggaaa gattctattc 5880
aaacaatcac ttggacccct gctaaaccca tatcttgaac acatatctaa gctttatgtt 5940
gcatggtctg gatctataga ggttaggttt tctatttctg gttctggtgt ttttggggga 6000
aaactcgctg ctattgttgt gccgcctggg gtagacccag tgcaaagcac atcaatgcta 6060
cagtatcctc atgttctatt tgatgcccgt caggtagaac ctgttatctt ttcaatacct 6120
gatttgagaa acagcttgta ccatcttatg tcagatactg atactacctc ccttgtaatt 6180
atggtgtaca atgatcttat taatccttat gcaaatgatt ctaactcttc tggatgtatt 6240
gtcactgttg agactaaacc tggaatcgat ttcaagtttc accttctgaa accccctgga 6300
tctatgctta cccatggctc tatcccttca gatttaatac caaaatcttc ttctctttgg 6360
atagggaatc gtcactggac tgacataacc gattttgtca ttaggccttt tgtgttccaa 6420
gcaaaccgtc attttgattt taatcaggaa acagctggtt ggagtacgcc aagattccga 6480
ccactagccg taacggttag ccaaagtaaa ggagcaaaat tggggaatgg tattgcgacc 6540
gactacatcg tgcctgggat tcctgatgga tggccagaca ctacaatccc aacaaaactg 6600
acacccacag gcgattatgc aattacatct agtgatggta atgacattga aacaaaactt 6660
gaatacgaaa atgctgatgt gattaaaaac aacacaaact tcaggagcat gtacatttgt 6720
gggtcgctcc aaagagcctg gggtgataag aagatttcta atactggttt cataactact 6780
ggtgttatct ctgataattc cataagccca agcaatacca ttgatcaaag caaaatcgtg 6840
gtctatcaag acaatcatgt caatagtgaa gttcaaacct cagacatcac acttgcaatt 6900
cttggctaca ctggtattgg tgaggaggca attggcgcca atcgggactc ggttgtacgc 6960
ataagtgtgc tgccagaaac aggtgcccgt gggggaaacc atccaatctt ttataagaat 7020
tctatgaagc ttggttacgt gatcagctcc attgatgtgt ttaattccca gatcttgcac 7080
acctctagac aactatcact gaataactac ttactacctc cagattcttt tgctgtctat 7140
cgaattattg attctaatgg gtcttggttt gatataggca ttgattctga tggattttct 7200
tttgttggtg tctcaagttt tcctaagtta gagtttccac tttctgcctc ctatatggga 7260
attcagttgg caaagattcg tcttgcctct aatattagga gttctatgac taaattatga 7320
attcaatatt aggcttaatt gacactgtta caaatacaat tggcaaagct caacaaattg 7380
aattagataa agctgcactt ggtcaacaac gcgaactggc tttgcaacga attaaccttg 7440
atcgccaggc gttgaacaac caagttgagc aatttaacaa aattcttgag caaagggtac 7500
aaggccccat ccagtctgtg cggctcgcac gtgctgctgg atttagggtt gacccttact 7560
catacacaaa tcaaaatttt tacgatgacc aattgaatgc aattagacta tcttatagga 7620
atttgtttaa ataattggcc acaagtatcc cttcgggctg ccgcatttgc gcctaacccc 7680
aggg 7684
<210> 63
<211> 24
<212> DNA
<213> 猫杯状病毒
<400> 63
catcgatcga tgtcgacgga tcca 24
<210> 64
<211> 25
<212> DNA
<213> 猫杯状病毒
<400> 64
gtgcatccgt cgacatcgat cgatg 25
<210> 65
<211> 45
<212> DNA
<213> 猫杯状病毒
<400> 65
tcgaggatcc gccaccatgg ccgatgatgg atcggtgaca acccc 45
<210> 66
<211> 46
<212> DNA
<213> 猫杯状病毒
<400> 66
tcgactcgag tcataattta gtcatagaac tcctaatatt agaggc 46

Claims (15)

1.与如SEQ ID NO:34中给出的氨基酸序列具有至少90％的序列同一性的猫杯状病毒(FCV)衣壳蛋白，其包含以下氨基酸：K90、M91、M101、I318、L391、A392、V393、Q397、S398、K399、N405、T427、T432、S439、S440、D441、E446、K448、L449、E452、N453、G485、G490、 I492、N517、S518、E519、I525、S546、S635、F636、P637。
2.包含根据权利要求1所述的衣壳蛋白的活减毒FCV。
3.DNA片段，其特征在于其包含编码根据权利要求1所述的衣壳蛋白的区域。
4.根据权利要求3所述的DNA片段，其特征在于将编码衣壳蛋白的区域置于合适启动子的控制下。
5.活减毒重组载体病毒(LARCV)，其特征在于所述载体病毒包含在合适启动子控制下的编码根据权利要求1所述的猫杯状病毒(FCV)衣壳蛋白的区域。
6.根据权利要求5所述的活减毒重组载体病毒，其特征在于所述LARCV是粘液瘤病毒或猫疱疹病毒。
7.根据权利要求5或6所述的活减毒重组载体病毒在制备药物中的用途，所述药物用于针对FCV保护猫。
8.活减毒杂合FCV，其特征在于所述FCV包含编码根据权利要求1所述的衣壳蛋白的开放阅读框2(ORF2)或根据权利要求3所述的DNA片段，并且包含来自减毒FCV的开放阅读框1(ORF1)。
9.根据权利要求8所述的活减毒杂合FCV，其特征在于所述FCV包含来自FCV毒株F9的ORF1。
10.根据权利要求8或9所述的活减毒杂合FCV在制备药物中的用途，所述药物用于针对FCV保护猫。
11.细胞培养物，其包含根据权利要求2所述的活减毒FCV、根据权利要求5或6所述的LARCV或根据权利要求8或9所述的活减毒杂合FCV。
12.用于针对FCV保护猫的疫苗，其特征在于所述疫苗包含根据权利要求2所述的活减毒FCV和/或根据权利要求5或6所述的活减毒重组载体病毒和/或根据权利要求8或9所述的活减毒杂合FCV以及药学上可接受的载体。
13.根据权利要求12所述的疫苗，其特征在于所述疫苗包含至少一种其他猫致病性微生物或猫致病性病毒和/或所述猫致病性微生物或猫致病性病毒的至少一种其他免疫原性组分和/或编码所述猫致病性微生物或猫致病性病毒的所述其他免疫原性组分的遗传物质。
14.根据权利要求13所述的疫苗，其特征在于所述至少一种其他猫致病性微生物或猫致病性病毒选自猫全白细胞减少症病毒、鹦鹉热衣原体(Chlamydia psittaci)、支气管炎博德特氏菌(Bordetella bronchiseptica)、猫细小病毒、狂犬病病毒和猫疱疹病毒。
15.用于获得根据权利要求8所述的活减毒杂合FCV的方法，其包括以下步骤：
a. 制备包含减毒FCV的完整ORF1区域和ORF2区域的相邻5’-部分的第一FCV扩增子，
b. 制备包含ORF1区域的3’-部分和完整的相邻ORF2//ORF3区域的第二FCV扩增子，其中ORF2是编码根据权利要求1所述的FCV衣壳蛋白的ORF2，
c. 使用重叠延伸组装第一和第二扩增子，
d. 生成感染性FCV，
e. 用感染性FCV感染易感细胞，
f. 回收感染性后代FCV。
CN202210237354.2A 2015-12-23 2016-12-22 猫杯状病毒疫苗 Pending CN114591405A (zh)

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