CN114557910B

CN114557910B - Lasting whitening antibacterial mouthwash and preparation method thereof

Info

Publication number: CN114557910B
Application number: CN202210367053.1A
Authority: CN
Inventors: 陈鑫; 孟渂偲
Original assignee: Xian Jiaotong University
Current assignee: Xian Jiaotong University
Priority date: 2022-04-08
Filing date: 2022-04-08
Publication date: 2023-04-28
Anticipated expiration: 2042-04-08
Also published as: CN114557910A

Abstract

The invention discloses a durable whitening antibacterial mouthwash and a preparation method thereof, comprising the steps of dissolving lysozyme, glucose oxidase and hemoglobin in Tris-HCl solution, and regulating pH to 7.5 to obtain a first solution; dissolving a mercaptan reducing agent in Tris-HCl solution, and regulating the pH value to 7.5 to obtain a second solution; and adding the second solution into the first solution, and uniformly stirring to obtain the mouthwash with the whitening and antibacterial functions. The mouthwash prepared by the invention has long-acting tooth whitening effect, and simultaneously the mouthwash can effectively sterilize and destroy the microenvironment on which the mouthwash depends, so that the antibacterial effect is more remarkable, and the mouthwash not only has good sterilizing effect on aerobic gram-positive bacteria and gram-negative bacteria on the surface of teeth, but also can kill anaerobic bacteria deep in the teeth.

Description

Lasting whitening antibacterial mouthwash and preparation method thereof

Technical Field

The invention relates to the technical field of daily chemicals, in particular to a durable whitening and sterilizing mouthwash and a preparation method thereof.

Background

In recent years, oral health has been increasingly emphasized, which has become one of the important indicators of the world health organization for evaluating the level of human health. Oral health depends on a good oral micro-ecosystem. However, various pathogenic bacteria introduced continuously in daily life will cause disturbance of this system. After entering the oral cavity, the bacteria can continuously absorb food and sugar in saliva to proliferate in a large amount, form stable dental plaque on the surface of teeth, secrete toxins and other harmful substances to destroy teeth and stimulate gums while affecting the appearance, thereby causing various oral diseases such as decayed teeth, periodontitis and pericoronitis, and further causing systemic diseases such as bacteremia, bacterial endocarditis, pneumonia, diabetes and the like. In addition, for people who ingest cigarettes, tea, coffee and the like for a long time, the continuous deposition of pigments on the surfaces of teeth not only further provides a growing place for bacteria, but also easily forms color spots, and the tooth attractiveness is affected. Thus, there is a need for a continuous effective bactericidal whitening strategy for oral health.

The gargle is one of the common ways of cleaning the oral cavity, can go deep into all parts of the oral cavity, and is expected to realize comprehensive antibacterial whitening effect. Meanwhile, the mouthwash is convenient to use and is beneficial to carrying, and has become an indispensable oral cavity cleaning and hygienic product in daily life. However, the dominant mouthwash in the market at present is sterilized by adding chlorine-containing compounds such as cetylpyridinium chloride, chlorhexidine gluconate and the like, so that the mouthwash has potential threat to human health, and the duration is extremely short, so that the antibacterial effect of the whole length cannot be realized. In addition, the main stream mouthwash only focuses on killing bacteria, does not focus on the problems of a warm bed such as saliva sugar, local anaerobic environment and the like on which the bacteria depend to survive, has limited antibacterial effect, and is difficult to effectively improve dental pigment deposition such as dental plaque, smoke tea stain and the like, and teeth whitening cannot be realized. Therefore, the construction of a mouthwash which is safe in components, can regulate and control bacterial growth microenvironment and realize long-acting antibacterial and whitening is expected to fill the blank of the mouthwash market.

Disclosure of Invention

Aiming at the problems existing in the prior art, the invention aims to provide the long-acting antibacterial and whitening mouthwash and the preparation method thereof, and the mouthwash prepared by the method has the effect of whitening teeth, is simple and feasible, can finish the reaction in one step, and can be used for mass production.

In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:

a collutory with skin whitening and antibacterial effects comprises lysozyme, glucose oxidase, hemoglobin and thiol reducer;

the mass ratio of lysozyme to glucose oxidase to hemoglobin is (1-3): (1-3): (1-3), the dosage ratio of lysozyme to thiol reducing agent is (1-3) g:50mmol.

A further improvement of the invention is that the thiol reducing agent is tris (2-carboxyethyl) phosphine hydrochloride.

A preparation method of mouthwash with whitening and antibacterial functions comprises the following steps:

1) Dissolving lysozyme, glucose oxidase and hemoglobin in Tris-HCl solution, and regulating pH to 7.5 to obtain a first solution;

dissolving a mercaptan reducing agent in Tris-HCl solution, and regulating the pH value to 7.5 to obtain a second solution;

2) And adding the second solution into the first solution, and uniformly stirring to obtain the mouthwash with the whitening and antibacterial functions.

The invention is further improved in that the mass ratio of lysozyme, glucose oxidase and hemoglobin is (1-3): (1-3): (1-3).

The invention is further improved in that the dosage ratio of lysozyme to thiol reducing agent is 1-3g:50mmol.

A further improvement of the invention is that the concentration of the Tris-HCl solution is 10mmol/L.

The invention is further improved in that the mercaptan reducing agent is tris (2-carboxyethyl) phosphine hydrochloride, and the concentration of the mercaptan reducing agent in the second solution is 45-55mmol/L.

The invention is further improved in that the concentration of lysozyme, glucose oxidase and hemoglobin in the first solution is 1-3mg/mL.

A further improvement of the invention is that the pH regulator is 5mol/L sodium hydroxide solution or sodium hydrogen phosphate solution.

Compared with the prior art, the invention has the beneficial effects that:

compared with the reported mouthwash, the mouthwash prepared by the invention has long-acting tooth whitening effect, and meanwhile, the mouthwash can effectively sterilize and destroy the microenvironment on which the mouthwash depends, so that the antibacterial effect is more remarkable, and the mouthwash not only has good sterilizing effect on aerobic gram-positive bacteria and gram-negative bacteria on the surface of the teeth, but also can kill anaerobic bacteria deep in the teeth.

The lysozyme, the hemoglobin and the glucose oxidase adopted by the invention are all natural components, so that the invention is green and safe, can be degraded and has no toxicity as degradation products. Compared with the prior art, the preparation method is simple, the operation process is simple, the mouthwash can be obtained simply through room temperature reaction, long-term and efficient tooth whitening and oral cavity harmful microorganism inhibition effects can be integrally realized, the reaction period is short, the yield is high, the working efficiency can be effectively improved, no organic waste liquid is generated in the operation process, the preparation method is environment-friendly, large-scale and expensive instruments and equipment are not needed, the production cost is low, and the industrial production is easy to realize. The mouthwash has good biocompatibility and environmental friendliness, has remarkable antibacterial effect, can prevent pigment deposition on the surfaces of teeth, and has wide market prospect.

Drawings

Fig. 1 is a schematic diagram of the preparation and action principle of the persistent whitening antibacterial mouthwash of the present invention.

Fig. 2 is an SEM image and elemental analysis image of the tooth surface treated with the mouthwash of example 2. Wherein, (a) is an SEM image, (b) is a C element, (C) is a Fe element, (d) is an N element, (e) is an S element, and (f) is a Merage image.

Fig. 3 is a statistical plot of the continuous release of oxygen by hemoglobin of the surface of the mouthwash treated teeth of example 3.

FIG. 4 is a statistical plot of the continuous consumption of ambient glucose by the mouthwash treated teeth of example 4, wherein (a) is a single rinse treated tooth used to simulate a single rinse; (b) Daily rinsing was simulated for the once daily mouthwash treatment of teeth.

Fig. 5 is a statistical plot of the continuous production of hydroxyl radicals by the mouthwash treated teeth of example 5 in the presence of glucose. The free radical indicator methyl violet is used to characterize the generation of free radicals. Wherein (a) is a single rinse treatment of teeth for simulating a single rinse; (b) Daily rinsing was simulated for the once daily mouthwash treatment of teeth.

Fig. 6 is a graph showing the inhibition of aerobic bacteria by the permanent whitening antibacterial mouthwash provided in example 6. Wherein (A) a paper sheet antibacterial physical diagram; and (B) is a statistical drawing of the bacteriostasis area.

Fig. 7 is a graph showing the inhibition of anaerobic bacteria by the permanent whitening antibacterial mouthwash provided in example 9.

Fig. 8 is a graph showing the inhibition of bacteria in the tooth environment before and after treatment with the mouthwash of example 7. Wherein (A) is a plate coating bacteriostasis physical image, and (B) is a bacteriostasis statistical image.

Fig. 9 is a graph showing the inhibition of bacteria on the tooth surface before and after the treatment of the mouthwash of example 8. Wherein (A) is a plate coating bacteriostasis physical image, and (B) is a bacteriostasis statistical image.

Fig. 10 is a graph of the whitening effect of the permanent whitening antibacterial mouthwash provided in example 10 on the teeth after dark extraction dyeing, wherein (a) is a real whitening graph and (b) is a statistical whitening graph.

Fig. 11 is a graph of the effect of preventing pigmentation of teeth after the treatment with the permanent whitening antibacterial mouthwash provided in example 11, wherein (a) is a physical graph of preventing redeposition and (b) is a statistical graph of preventing pigmentation.

Detailed Description

The invention is further illustrated, but not limited, by the following examples and figures.

According to the invention, natural macromolecules (egg white lysozyme, glucose oxidase and hemoglobin) are used as main components of the mouthwash, and the whitening antibacterial mouthwash is prepared through one-step reaction.

The invention provides application of the mouth wash containing egg white lysozyme, glucose oxidase and hemoglobin in resisting bacterial infection and whitening teeth, wherein the bacteria are aerobic and anaerobic gram-positive bacteria and gram-negative bacteria.

Referring to fig. 1, the preparation method of the mouthwash with whitening and antibacterial functions of the present invention includes the following steps, but is not limited to the following steps:

1) Lysozyme, glucose oxidase and hemoglobin were dissolved in Tris-HCl solution, and pH was adjusted to 7.5 with pH adjuster to obtain a first solution. The lysozyme is derived from egg white. The concentration of lysozyme in the first solution is 1-3mg/mL, the concentration of glucose oxidase is 1-3mg/mL, and the concentration of hemoglobin is 1-3mg/mL. The pH was adjusted to 7.5 because the isoelectric point of lysozyme was 7.5.

The pH regulator is sodium hydroxide solution or sodium hydrogen phosphate solution, and the concentration is 5mol/L.

Dissolving a mercaptan reducing agent in Tris-HCl solution, and regulating the pH value to obtain a second solution. In the second solution, the concentration of the mercaptan reducing agent is 45-55mmol/L.

The concentration of the Tris-HCl solution is 10mmol/L.

The thiol reducing agent is tris (2-carboxyethyl) phosphine hydrochloride, and other thiol reducing agents have a large taste and are easily oxidized, so that the thiol reducing agent is not suitable.

2) Adding the second solution into the first solution, and stirring at room temperature for 1-10s to uniformly mix to obtain the mouthwash with whitening and antibacterial functions.

The mouthwash prepared by the invention can rapidly form a nano film containing lysozyme, glucose oxidase and hemoglobin on the surface of teeth, and can play the roles of whitening and antibiosis for a long time.

The mechanism of the nano film formation of the invention is as follows: under the action of thiol reducing agent, natural lysozyme has conformational change to form high-adhesiveness amyloid protein aggregation, and further assembles with hemoglobin and glucose oxidase to form nano film.

The mechanism of the invention for resisting bacteria and whitening skin is as follows: hemoglobin continuously captures and provides oxygen, improves the anoxic microenvironment of the oral cavity, and inhibits the growth of anaerobic harmful bacteria. The oxygen provided by the method further acts together with glucose oxidase to oxidize glucose in saliva, cuts off the food source of bacteria, and inhibits the growth of anaerobic and aerobic harmful bacteria. Hydrogen peroxide is generated simultaneously in the oxidation process of glucose, and the hydrogen peroxide can be further catalyzed into hydroxyl radicals by ferrous elements in hemoglobin. The hydroxyl radical with high oxidability has a very strong sterilization effect, so that anaerobic and aerobic harmful bacteria can be killed efficiently. Meanwhile, the hydroxyl free radical with high oxidability can quickly oxidize pigment, so that the whitening effect is achieved. The functions can be realized for a long time due to the stable existence of the nano film on the tooth surface.

Example 1

0.004g of lysozyme, 0.004g of hemoglobin and 0.004g of glucose oxidase were each weighed and dissolved in 2mL (10 mmol/LTris-HCl) of solution, and the pH of the solution was adjusted to 7.5 using 5mol/L sodium hydroxide solution.

200. Mu.L (0.5 mol/L, pH=6.8) of Tris (2-carboxyethyl) phosphine hydrochloride solution was weighed into 1800. Mu.L (10 mmol/L Tris-HCl), and the solution pH was adjusted using 5mol/L sodium hydrogen phosphate solution so that the concentration of Tris (2-carboxyethyl) phosphine hydrochloride became 50mmol/L.

Mixing the tris (2-carboxyethyl) phosphine hydrochloride solution with the lysozyme solution containing glucose oxidase and hemoglobin according to the volume ratio of 1:1, and repeatedly sucking for several times by using a pipette gun to fully mix the solutions to obtain the mouthwash.

Preferably, in the embodiment of the present invention, the concentration of lysozyme in the first solution is 2mg/mL, ph=7.5; tris (2-carboxyethyl) phosphine hydrochloride concentration 50mmol/L, ph=7.5; glucose concentration was 25mmol/L; the concentration of hemoglobin is 2mg/mL; the glucose oxidase concentration was 2mg/mL.

Example 2

The mouthwash of example 1 was used to soak teeth, left to stand at room temperature for 1min, and the teeth rinsed with deionized water. Vacuum drying at 37deg.C for 16h, and testing surface structure and chemical composition by scanning electron microscope and elemental analysis. As can be seen from fig. 2 (a), (b), (c), (d), (e) and (f), the mouthwash forms a nano-film consisting of 200nm nano-spheres consisting of hemoglobin, glucose oxidase and lysozyme on the tooth surface.

Example 3

1000. Mu.L of PBS was purged with nitrogen for 10min, followed by placing the mouthwash-treated teeth in PBS, and sealing by adding 1000. Mu.L of paraffin solution. A dissolved oxygen detector was used to monitor the change in oxygen in the solution. Referring to fig. 3, it is known from the analysis result that the hemoglobin covered on the tooth surface treated with the mouthwash can effectively release oxygen under the condition of hypoxia, thereby improving the hypoxia environment.

Example 4

The mouthwash of example 1 was taken to soak 2 teeth, left to stand at room temperature for 1min, and the teeth were rinsed with deionized water. Teeth were placed in solutions with a glucose concentration of 25mm/L, respectively, and recorded as solution A, solution B, respectively. After one day, the glucose content of the A, B solution was measured using a blood glucose meter. The teeth in solution B were then removed and the mouthwash of example 1 was used to soak the teeth and allowed to stand at room temperature for 1min. After rinsing the teeth with deionized water, it was placed in solution B. After one day, the above procedure was repeated, and the single rinsing process was simulated separately with the A experiment, and the B experiment simulated one rinsing process per day. Referring to fig. 4 (a) and (b), it is understood from the analysis results that a film capable of continuously consuming glucose can be formed by rinsing only once, and the film function can be enhanced by rinsing every day, and more glucose is consumed.

Example 5

A0.0005 g/mL methyl violet (free radical indicator) solution was prepared, followed by glucose addition to a glucose concentration of 25mm/L. 2 teeth were immersed in the mouthwash of example 1, and left to stand at room temperature for 1min, and the teeth were rinsed with deionized water. The teeth were placed in the methyl violet solutions, and the methyl violet in the solutions was detected using an ultraviolet spectrophotometer and recorded as solution a, solution B, respectively. After one day, the methyl violet content of the A, B solution was detected using an ultraviolet spectrophotometer. Taking out the teeth in the solution B, taking the mouthwash in the first embodiment to soak and change the teeth, and standing for 1min at room temperature. After rinsing the teeth with deionized water, it was placed in solution B. After one day, the above procedure was repeated, and the single rinsing process was simulated separately with the A experiment, and the B experiment simulated one rinsing process per day. Referring to fig. 5 (a) and (b), it is known from the analysis result that a nano film capable of continuously generating hydroxyl radicals by consuming glucose can be formed by rinsing the mouth once, and glucose oxidase can be continuously supplemented every day, so that the generation efficiency of the hydroxyl radicals can be continuously enhanced.

Example 6

A sheet of 6mm paper was prepared, autoclaved and oven dried at 80 ℃. The paper pieces were immersed in the mouthwash solution of example one. Preparing glucose solution with the concentration of 25mm/L to dilute the bacterial solution to 1 x 10 ⁵ CFU/g of E.coli (ACTCC 25922) and Staphylococcus aureus (ATCC 29213) were uniformly spread on the solid medium. The paper sheet soaked in the mouthwash is placed on a solid culture medium full of bacteria and incubated at 37℃for 12 hours. Referring to FIGS. 6 (A) and (B), using the measuring tool, a mouthwash-containing sheet of paper was found to form approximately 13.04cm on a solid medium of Staphylococcus aureus ² About the zone of inhibition, the paper sheet containing the mouthwash forms about 5.87cm on the solid medium of the escherichia coli ² A left bacteriostasis ring and a right bacteriostasis ring.

Example 7

According to the culture method of anaerobic bacteria, respectively adding a mouthwash solution into the anaerobic bacteria solution, monitoring the OD 600 value of the bacteria solution every day, and according to the formula:

where A1 is the OD 600 value of the sample set and A0 is the initial OD 600 value of the sample set. B1 is the OD 600 value of the blank group, B0 is the initial OD 600 value of the blank group, and C is the bacteriostasis rate. Referring to fig. 8 (a) and (B), it is known from the analysis result that the mouthwash can change the anaerobic environment by hemoglobin, and the carried oxygen can promote the conversion of glucose, thereby promoting the generation of hydroxyl radicals, improving the anaerobic environment and thus killing anaerobic bacteria.

Example 8

The tooth was immersed in the mouthwash of example 1, then placed in the bacterial liquid of example six, and after the tooth was left at 37℃for 72 hours, 10. Mu.L of the bacterial liquid was taken out and uniformly spread on a solid medium. Referring to fig. 9 (a) and (B), it is clear from the analysis results that the antibacterial rate against gram-positive bacteria and the antibacterial rate against gram-negative bacteria of the teeth treated with the mouthwash can reach 99.73148% and 96.79428%.

Example 9

After the tooth of example 7 was removed, bacteria on the tooth surface were removed by using sterilized water, and then 2mL of sterilized water was used to thoroughly shake the tooth, and bacteria on the tooth surface were shaken off. 10. Mu.L of the solution was applied to the solid medium. Referring to fig. 7, it is known that the surface of the plastic sheet is treated with the coating solution to effectively kill bacteria and prevent bacterial infection.

Example 10

The teeth were immersed in the high-concentration dark extraction solution for 15 days to simulate staining of the teeth. Preparing PBS solution containing glucose 25mm/L, treating stained teeth with mouthwash, and placing the treated teeth into PBS solution containing glucose. The experimental group is the group for carrying out mouthwash treatment on teeth, and the control group is the group for not treating dyed teeth. After one day, the teeth in the experimental group were removed and treated again with mouthwash. The above operation is repeated. Referring to fig. 10, the course of tooth change was recorded using a camera, and quantitative analysis statistics were performed on the photographs using the international commission on illumination Lab mode. Wherein the W value is defined as the degree of tooth whitening;

W＝L-a-b

from this, it can be seen that: the tooth treated by the mouthwash can effectively remove pigment on the tooth.

Example 11

The stained teeth were immersed in the mouthwash solution, and the treated teeth were then placed in the rhodamine solution of example 5. The experimental group is the tooth group after the coating treatment, and the control group is the control group which is not treated by using the mouthwash. After one day, the teeth in the experimental group were removed and treated again with mouthwash. The above operation is repeated. The course of tooth changes were recorded using a camera and quantitative analysis statistics were performed on the photographs using the international committee of illumination Lab model. Wherein the W value is defined as the whitening degree;

W＝L-a-b

referring to fig. 11 (a) and (b), it is understood from the results that the nano-film formed on the tooth surface after the mouthwash treatment can prevent the redeposition of pigment.

Example 12

1) Lysozyme (0.004 g), glucose oxidase and hemoglobin are dissolved in 10mmol/L Tris-HCl solution, and 5mol/L sodium hydrogen phosphate solution is adopted to adjust the pH value to 7.5, so as to obtain a first solution; wherein the concentration of lysozyme, glucose oxidase and hemoglobin in the first solution is 1mg/mL;

dissolving Tris (2-carboxyethyl) phosphine hydrochloride in 10mmol/L Tris-HCl solution, and regulating the pH to 7.5 by adopting 5mol/L sodium hydrogen phosphate solution to obtain a second solution with the concentration of 45 mmol/L;

2) And adding the second solution into the first solution, and uniformly stirring to obtain the mouthwash with the whitening and antibacterial functions. Wherein, the dosage ratio of lysozyme to tris (2-carboxyethyl) phosphine hydrochloride is 1g:50mmol.

Example 13

1) Lysozyme (0.004 g), glucose oxidase and hemoglobin are dissolved in 10mmol/L Tris-HCl solution, and 5mol/L sodium hydrogen phosphate solution is adopted to adjust the pH value to 7.5, so as to obtain a first solution; wherein the concentration of lysozyme, glucose oxidase and hemoglobin in the first solution is 2mg/mL;

dissolving Tris (2-carboxyethyl) phosphine hydrochloride in 10mmol/L Tris-HCl solution, and regulating the pH to 7.5 to obtain a second solution with the concentration of 55mmol/L;

2) And adding the second solution into the first solution, and uniformly stirring to obtain the mouthwash with the whitening and antibacterial functions. Wherein, the dosage ratio of lysozyme to tris (2-carboxyethyl) phosphine hydrochloride is 2g:50mmol.

Example 14

1) Lysozyme (0.004 g), glucose oxidase and hemoglobin are dissolved in 10mmol/L Tris-HCl solution, and 5mol/L sodium hydrogen phosphate solution is adopted to adjust the pH value to 7.5, so as to obtain a first solution; wherein the concentration of lysozyme, glucose oxidase and hemoglobin in the first solution is 3mg/mL;

dissolving Tris (2-carboxyethyl) phosphine hydrochloride in 10mmol/L Tris-HCl solution, and regulating the pH to 7.5 by adopting 5mol/L sodium hydrogen phosphate solution to obtain a second solution with the concentration of 55mmol/L;

2) And adding the second solution into the first solution, and uniformly stirring to obtain the mouthwash with the whitening and antibacterial functions. Wherein, the dosage ratio of lysozyme to tris (2-carboxyethyl) phosphine hydrochloride is 3g:50mmol.

Example 15

1) Lysozyme (0.004 g), glucose oxidase and hemoglobin are dissolved in 10mmol/L Tris-HCl solution, and 5mol/L sodium hydroxide solution is adopted to adjust the pH value to 7.5, so as to obtain a first solution; wherein the concentration of lysozyme in the first solution is 1mg/mL; the concentration of glucose oxidase in the first solution was 2mg/mL, and the concentration of hemoglobin in the first solution was 3mg/mL.

Dissolving Tris (2-carboxyethyl) phosphine hydrochloride in 10mmol/L Tris-HCl solution, and adopting 5mol/L sodium hydroxide solution to adjust the pH value to 7.5 to obtain a second solution with the concentration of 52 mmol/L;

Claims

1. A mouthwash with whitening and antibacterial functions is characterized by comprising lysozyme, glucose oxidase, hemoglobin and a mercaptan reducing agent;

the mass ratio of lysozyme to glucose oxidase to hemoglobin is (1-3): (1-3): (1-3), the dosage ratio of lysozyme to thiol reducing agent is (1-3) g:50mmol;

the mercaptan reductant is tris (2-carboxyethyl) phosphine hydrochloride;

wherein the concentration of the mercaptan reducing agent is 45-55mmol/L;

the concentration of lysozyme, glucose oxidase and hemoglobin is 1-3mg/mL.

2. The preparation method of the mouthwash with the whitening and antibacterial functions is characterized by comprising the following steps of:

2) Adding the second solution into the first solution, and uniformly stirring to obtain the mouthwash with whitening and antibacterial functions;

the mass ratio of lysozyme, glucose oxidase to hemoglobin is (1-3): (1-3): (1-3); the mercaptan reductant is tris (2-carboxyethyl) phosphine hydrochloride;

the dosage ratio of lysozyme to thiol reducer is 1-3g:50mmol;

the concentration of the mercaptan reducing agent in the second solution is 45-55mmol/L;

the concentration of lysozyme, glucose oxidase and hemoglobin in the first solution is 1-3mg/mL.

3. The method for preparing the mouthwash with whitening and antibacterial functions according to claim 2, wherein the concentration of the Tris-HCl solution is 10mmol/L.

4. The method for preparing the mouthwash with whitening and antibacterial functions according to claim 2, wherein the pH regulator is 5mol/L sodium hydroxide solution or sodium hydrogen phosphate solution.