CN114540244A - Growth-promoting bacteria and microbial fertilizer for promoting growth of Chinese fir - Google Patents
Growth-promoting bacteria and microbial fertilizer for promoting growth of Chinese fir Download PDFInfo
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- CN114540244A CN114540244A CN202210263591.6A CN202210263591A CN114540244A CN 114540244 A CN114540244 A CN 114540244A CN 202210263591 A CN202210263591 A CN 202210263591A CN 114540244 A CN114540244 A CN 114540244A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention provides growth-promoting bacteria for promoting the growth of China fir, which comprise bacillus licheniformis, bacillus stearothermophilus and bacillus lysimachiae in a weight ratio of 3: 2: 1. the microbial fertilizer adopts growth-promoting bacteria consisting of bacillus licheniformis, bacillus stearothermophilus and bacillus lysinate to be matched with other raw materials for use. The bacillus is used as an active agent, can secrete a large amount of organic acid, dissolves inorganic phosphorus, improves the absorption and utilization of phosphorus by plants, is particularly suitable for the production of forest biological bacterial fertilizers and forest growth promoting microbial inoculum in red soil areas in southeast of Guidong, and can be further developed and developed into microbial compound fertilizers and microbial inoculum. The blended nutrient solution contains various nutrient elements, so that the nutrient composition of the product is improved, and meanwhile, the calcined shell powder is added, so that the activity is enhanced, the raw materials are combined and proportioned, and the fertilizer efficiency of the product is improved.
Description
Technical Field
The invention relates to the technical field of microbial fertilizers, in particular to growth-promoting bacteria for promoting the growth of China fir and a microbial fertilizer.
Background
The biological organic fertilizer is a fertilizer which is compounded by microorganisms with specific functions and organic materials mainly prepared from animal and plant residues (such as livestock and poultry manure, crop straws and the like) through harmless treatment and decomposition. Beneficial microorganisms in the bio-organic fertilizer enter soil and then form a symbiotic proliferation relationship with microorganisms in the soil, inhibit the growth of harmful bacteria and convert the harmful bacteria into beneficial bacteria, promote and influence each other, and play a role in group synergy.
The microbial inoculum adopted by the existing microbial fertilizer is simple in proportioning, not strong in adaptability to China fir rhizosphere and not high in colonization activity, the growth promotion effect is not obvious, and further improvement treatment is needed.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide growth-promoting bacteria and a microbial fertilizer for promoting the growth of China fir.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the invention provides growth-promoting bacteria for promoting the growth of China fir, which comprise bacillus licheniformis, bacillus stearothermophilus and bacillus lysimachiae in a weight ratio of 3: 2: 1.
the microbial fertilizer comprises the following raw materials in parts by weight:
10-30 parts of growth-promoting bacteria, 10-20 parts of nutrient solution, 5-10 parts of modified diatomite, 5-10 parts of straw ash, 1-5 parts of calcined shell powder and 1-3 parts of livestock manure.
Preferably, the microbial fertilizer comprises the following raw materials in parts by weight:
10-30 parts of growth-promoting bacteria, 10-20 parts of nutrient solution, 5-10 parts of modified diatomite, 5-10 parts of straw ash, 1-5 parts of calcined shell powder and 1-3 parts of livestock manure.
Preferably, the calcining method for calcining the shell powder comprises the following steps: the method comprises the steps of feeding shell powder into a 30-40% by weight of horse hydrochloric acid solution according to the weight ratio of 1:3, then washing with water, drying, calcining at 600 ℃ for 10-20min, cooling to room temperature after calcining, and then carrying out heat treatment.
Preferably, the specific operation steps of the heat treatment are as follows: the temperature is firstly increased to 350-450 ℃ at the speed of 1-3 ℃/min, the reaction is carried out for 20-30min, then the temperature is increased to 600 ℃ at the speed of 5 ℃/min, the temperature is continuously kept for 10-20min, and finally the temperature is naturally cooled to the room temperature.
Preferably, the preparation method of the nutrient solution comprises the following steps:
s1: dissolving sodium selenite in water to obtain stock solution with sodium selenite concentration of 30-50 g/l;
s2: mixing urea, diammonium hydrogen phosphate, potassium dihydrogen phosphate and sodium selenite solution according to a volume ratio of 1 (2-3), and then carrying out oscillation chelation at 45 ℃ for 40-50 h;
s3: and (3) reacting the S2 product with the modified bentonite, and obtaining the nutrient solution after the reaction is finished.
Preferably, the modified bentonite modification method comprises the following steps: and (2) delivering the bentonite into a calcining furnace for calcining at the calcining temperature of 500 ℃ for 10-20min, cooling to 40 ℃ after the calcining is finished, then stirring in an activating solution, washing with water after the stirring is finished, and drying to obtain the modified bentonite.
Preferably, the activating solution is lanthanum chloride and hydrochloric acid in a weight ratio of 1:3, and the raw materials are stirred until the raw materials are fully mixed to obtain the activating solution.
Preferably, the activation solution is stirred at a rotation speed of 100-500r/min for 10-20 min.
Preferably, the stirring speed is 300r/min, and the stirring time is 15 min.
Compared with the prior art, the invention has the following beneficial effects:
the microbial fertilizer adopts growth-promoting bacteria consisting of bacillus licheniformis, bacillus stearothermophilus and bacillus lysinate to be matched with other raw materials for use. The bacillus is used as an active agent, can secrete a large amount of organic acid, dissolves inorganic phosphorus, improves the absorption and utilization of phosphorus by plants, is particularly suitable for the production of forest biological bacterial fertilizers and forest growth promoting microbial inoculum in red and yellow soil areas in southeast of Guidong, and can be further developed and developed into microbial compound fertilizers and microbial inoculum. The blended nutrient solution contains various nutrient elements, so that the nutrient composition of the product is improved, and meanwhile, the calcined shell powder is added, so that the activity is enhanced, the raw materials are combined and proportioned, and the fertilizer efficiency of the product is improved.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1.
The growth-promoting bacteria for promoting the growth of the fir comprise bacillus licheniformis, bacillus stearothermophilus and bacillus lysimachiae, and the weight ratio of the growth-promoting bacteria to the bacillus licheniformis to the bacillus stearothermophilus is 3: 2: 1.
the microbial fertilizer comprises the following raw materials in parts by weight:
10 parts of growth-promoting bacteria, 10 parts of nutrient solution, 5 parts of modified diatomite, 5 parts of straw ash, 1 part of calcined shell powder and 1 part of livestock manure.
The calcination method for calcining the shell powder in the embodiment is as follows: and (2) feeding the shell powder into a 30 mass percent solution of horse hydrochloric acid according to the weight ratio of 1:3, then washing with water, drying, calcining at 500 ℃ for 10min, cooling to room temperature after the calcination is finished, and then carrying out heat treatment to finish the treatment.
The specific operation steps of the heat treatment in this embodiment are: the temperature is firstly increased to 350 ℃ at the speed of 1 ℃/min, the reaction is carried out for 20min, then the temperature is increased to 600 ℃ at the speed of 5 ℃/min, the heat preservation is continued for 10min, and finally the natural cooling is carried out to the room temperature.
The preparation method of the nutrient solution of the embodiment comprises the following steps:
s1: dissolving sodium selenite in water to prepare an original mother solution, wherein the concentration of the sodium selenite is 30 g/l;
s2: mixing urea, diammonium hydrogen phosphate, potassium dihydrogen phosphate and sodium selenite solution in a volume ratio of 1:2, and then carrying out oscillation chelation for 40h at 45 ℃;
s3: and (3) reacting the S2 product with the modified bentonite, and obtaining the nutrient solution after the reaction is finished.
The modification method of the modified bentonite in this example is: and (2) delivering the bentonite into a calcining furnace for calcining at the calcining temperature of 500 ℃ for 10min, cooling to 40 ℃ after the calcining is finished, then stirring in an activating solution, washing with water after the stirring is finished, and drying to obtain the modified bentonite.
The activating solution of this example is lanthanum chloride and hydrochloric acid in a weight ratio of 1:3, and is obtained by stirring the raw materials until the raw materials are fully mixed.
The activating solution of this example was stirred at a rotation speed of 100r/min for 10 min.
Example 2.
The growth-promoting bacteria for promoting the growth of the fir comprise bacillus licheniformis, bacillus stearothermophilus and bacillus lysimachiae, and the weight ratio of the growth-promoting bacteria to the bacillus licheniformis to the bacillus stearothermophilus is 3: 2: 1.
the microbial fertilizer comprises the following raw materials in parts by weight:
30 parts of growth-promoting bacteria, 20 parts of nutrient solution, 10 parts of modified diatomite, 10 parts of straw ash, 5 parts of calcined shell powder and 3 parts of livestock manure.
The calcination method for calcining the shell powder in the embodiment is as follows: and (2) feeding the shell powder into a 40 mass percent solution of horse hydrochloric acid according to the weight ratio of 1:3, then washing with water, drying, calcining at 600 ℃ for 20min, cooling to room temperature after the calcination is finished, and then carrying out heat treatment to finish the treatment.
The specific operation steps of the heat treatment in this embodiment are: the temperature is firstly increased to 450 ℃ at the speed of 3 ℃/min, the reaction is carried out for 30min, then the temperature is increased to 600 ℃ at the speed of 5 ℃/min, the heat preservation is continued for 20min, and finally the temperature is naturally cooled to the room temperature.
The preparation method of the nutrient solution of the embodiment comprises the following steps:
s1: dissolving sodium selenite in water to prepare an original mother solution, wherein the concentration of the sodium selenite is 50 g/l;
s2: mixing urea, diammonium hydrogen phosphate, potassium dihydrogen phosphate and sodium selenite solution in a volume ratio of 1:3, and then carrying out oscillation chelation for 50h at 45 ℃;
s3: and (3) reacting the S2 product with the modified bentonite, and obtaining the nutrient solution after the reaction is finished.
The modification method of the modified bentonite in this example is: and (2) delivering the bentonite into a calcining furnace for calcining at the calcining temperature of 500 ℃ for 20min, cooling to 40 ℃ after the calcining is finished, then stirring in an activating solution, washing with water after the stirring is finished, and drying to obtain the modified bentonite.
The activating solution of this example is lanthanum chloride and hydrochloric acid in a weight ratio of 1:3, and is obtained by stirring the raw materials until the raw materials are fully mixed.
The activating solution of this example was stirred at a speed of 500r/min for 20 min.
Example 3.
The growth-promoting bacteria for promoting the growth of the Chinese fir comprise bacillus licheniformis, bacillus stearothermophilus and bacillus lysimachiae in a weight ratio of 3: 2: 1.
the microbial fertilizer comprises the following raw materials in parts by weight:
10-30 parts of growth-promoting bacteria, 10-20 parts of nutrient solution, 5-10 parts of modified diatomite, 5-10 parts of straw ash, 1-5 parts of calcined shell powder and 1-3 parts of livestock manure.
The calcination method for calcining the shell powder in the embodiment is as follows: and (2) feeding the shell powder into a 35% by weight of maleic acid solution according to the weight ratio of 1:3, then washing with water, drying, calcining at 550 ℃ for 10-20min, cooling to room temperature after the calcination is finished, and then carrying out heat treatment to finish the treatment.
The specific operation steps of the heat treatment in this embodiment are: heating the temperature to 400 ℃ at the speed of 2 ℃/min, reacting for 25min, heating to 600 ℃ at the speed of 5 ℃/min, keeping the temperature for 10-20min, and naturally cooling to room temperature.
The preparation method of the nutrient solution of the embodiment comprises the following steps:
s1: dissolving sodium selenite in water to prepare an original mother solution, wherein the concentration of the sodium selenite is 40 g/l;
s2: mixing urea, diammonium hydrogen phosphate, potassium dihydrogen phosphate and sodium selenite solution in a volume ratio of 1:2.5, and then carrying out oscillation chelation at 45 ℃ for 45 hours;
s3: and (3) reacting the S2 product with the modified bentonite, and obtaining the nutrient solution after the reaction is finished.
The modification method of the modified bentonite in this example is: and (2) delivering the bentonite into a calcining furnace for calcining at the calcining temperature of 500 ℃ for 15min, cooling to 40 ℃ after the calcining is finished, then stirring in an activating solution, washing with water after the stirring is finished, and drying to obtain the modified bentonite.
The activating solution of this example is lanthanum chloride and hydrochloric acid in a weight ratio of 1:3, and is obtained by stirring the raw materials to mix thoroughly.
The activating solution of this example was stirred at a rotation speed of 300r/min for 15 min.
Comparative example 1.
The only difference from example 3 is that no modified bentonite was added.
The product properties of examples 1-3 and comparative example 1 were tested as follows:
it will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. The growth-promoting bacteria for promoting the growth of the Chinese fir are characterized by comprising bacillus licheniformis, bacillus stearothermophilus and bacillus lysimachiae in a weight ratio of 3: 2: 1.
2. the microbial fertilizer according to claim 1, which comprises the following raw materials in parts by weight:
10-30 parts of growth-promoting bacteria, 10-20 parts of nutrient solution, 5-10 parts of modified diatomite, 5-10 parts of straw ash, 1-5 parts of calcined shell powder and 1-3 parts of livestock manure; and soaking the modified diatomite in hydrochloric acid for modification treatment to obtain the modified diatomite.
3. The microbial fertilizer according to claim 1, which comprises the following raw materials in parts by weight:
10-30 parts of growth-promoting bacteria, 10-20 parts of nutrient solution, 5-10 parts of modified diatomite, 5-10 parts of straw ash, 1-5 parts of calcined shell powder and 1-3 parts of livestock manure.
4. A microbial fertilizer according to claim 1, wherein the calcining method of the calcined shell powder comprises the following steps: the method comprises the steps of feeding shell powder into a 30-40 wt% equine hydrochloric acid solution according to the weight ratio of 1:3, then washing with water, drying, calcining at the temperature of 500-600 ℃ for 10-20min, cooling to room temperature after calcining, and then carrying out heat treatment.
5. A microbial fertilizer according to claim 4, wherein the heat treatment comprises the following specific steps: the temperature is firstly increased to 350-450 ℃ at the speed of 1-3 ℃/min, the reaction is carried out for 20-30min, then the temperature is increased to 600 ℃ at the speed of 5 ℃/min, the temperature is continuously kept for 10-20min, and finally the temperature is naturally cooled to the room temperature.
6. The microbial fertilizer according to claim 1, wherein the preparation method of the nutrient solution comprises the following steps:
s1: dissolving sodium selenite in water to obtain stock solution with sodium selenite concentration of 30-50 g/l;
s2: mixing urea, diammonium hydrogen phosphate, potassium dihydrogen phosphate and sodium selenite solution according to a volume ratio of 1 (2-3), and then carrying out oscillation chelation at 45 ℃ for 40-50 h;
s3: and (3) reacting the S2 product with the modified bentonite, and obtaining the nutrient solution after the reaction is finished.
7. The microbial fertilizer according to claim 6, wherein the modified bentonite is modified by the following method: and (2) delivering the bentonite into a calcining furnace for calcining at the calcining temperature of 500 ℃ for 10-20min, cooling to 40 ℃ after the calcining is finished, then stirring in an activating solution, washing with water after the stirring is finished, and drying to obtain the modified bentonite.
8. The microbial fertilizer as claimed in claim 7, wherein the activating solution is lanthanum chloride and hydrochloric acid in a weight ratio of 1:3, and is obtained by stirring the raw materials and fully mixing the raw materials.
9. The microbial fertilizer as claimed in claim 7, wherein the activation solution is stirred at a rotation speed of 100-500r/min for 10-20 min.
10. A microbial fertilizer according to claim 9, wherein the stirring speed is 300r/min and the stirring time is 15 min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210263591.6A CN114540244A (en) | 2022-03-17 | 2022-03-17 | Growth-promoting bacteria and microbial fertilizer for promoting growth of Chinese fir |
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| CN202210263591.6A CN114540244A (en) | 2022-03-17 | 2022-03-17 | Growth-promoting bacteria and microbial fertilizer for promoting growth of Chinese fir |
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| CN111440616A (en) * | 2020-04-27 | 2020-07-24 | 徐州生物工程职业技术学院 | Saline-alkali soil conditioner and preparation method thereof |
| CN112209778A (en) * | 2020-09-26 | 2021-01-12 | 上海康恒环境修复有限公司 | Novel slag soil conditioner and preparation method thereof |
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2022
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Patent Citations (8)
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| CN103351188A (en) * | 2013-08-14 | 2013-10-16 | 苏州荣基生态生物科技有限公司 | Preparation method of agricultural organic fertilizer by treating pure straws as raw material |
| CN106116807A (en) * | 2016-06-21 | 2016-11-16 | 刘月华 | A kind of ornamental trees and shrubs dedicated fertilizer and preparation technology thereof |
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