CN114532371A

CN114532371A - Combined preparation method of natural compound plant disinfectant

Info

Publication number: CN114532371A
Application number: CN202210147541.1A
Authority: CN
Inventors: 李小兵; 康颖倩; 程敏
Original assignee: Guizhou Bailejin Biotechnology Co ltd
Current assignee: Guizhou Bailejin Biotechnology Co ltd
Priority date: 2022-02-17
Filing date: 2022-02-17
Publication date: 2022-05-27

Abstract

The invention relates to the technical field of microbial killing, in particular to a combined preparation method of a natural compound plant disinfectant. The composition for the disinfectant comprises a traditional Chinese medicine composition for destroying cell walls of gram-positive bacteria and a chemical disinfectant, wherein in the sterilization process, the traditional Chinese medicine composition firstly destroys the cell walls of the gram-positive bacteria so that the chemical disinfectant can smoothly enter the bacteria to realize quick sterilization.

Description

Combined preparation method of natural compound plant disinfectant

Technical Field

The invention relates to the technical field of microbial killing, in particular to a combined preparation method of a natural compound plant disinfectant.

Background

Disinfection and sterilization are necessary for people to maintain health. Sterilization includes various methods of physical, biological, and chemical means. The effective and commonly used physical disinfection methods mainly include ultraviolet disinfection, high-temperature high-pressure disinfection, ultrasonic disinfection, air purification by filter membrane filtration and the like. At present, a common ultraviolet lamp is an open lamp column type, the risk is uncontrollable, and the generated harmful substances can cause damage to eyes and skin of a human body. The biological sterilization method is a sterilization method which has appeared in recent years, and the purpose of killing microorganisms is achieved mainly by using some substances (such as bacteriophage, antibacterial peptide, biological enzyme and the like) produced by organisms. The biological disinfection device can inhibit or kill pests, does not continuously damage the environment, but has the problems of uncertain disinfection effect, low disinfection efficiency, difficulty in large-scale application and the like. The chemical disinfection method mainly achieves the purpose of killing pathogenic microorganisms through the redox property of chemical agents. The chemical disinfectants commonly used at present comprise chlorine disinfectants, oxidation disinfectants, iodine disinfectants, aldehyde disinfectants, heterocyclic gas disinfectants, phenol disinfectants, alcohol disinfectants, quaternary amine disinfectants and the like. The chemical disinfectant has the advantages of various varieties, convenient operation, simple facilities, wide application range and the like, and is widely applied to the sanitary disinfection of hospitals, hotels, tourism, food processing industries, families, epidemic sources and the like. However, excessive use of chemical disinfectants can not only harm human health and destroy the environment, but also cause drug residues and increase the drug resistance of pathogenic microorganisms.

Gram-positive bacteria are important components of a plurality of drug-resistant microorganisms due to the special cell wall structure, so that a disinfectant which has a good disinfection effect on gram-positive bacteria, is environment-friendly and does not increase the drug resistance of microorganisms is urgently needed to be developed.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a composition for a disinfectant, which takes traditional Chinese medicines as main raw materials, and is added with a chemical disinfectant to realize compounding, so that the disinfection effect is further improved on the premise of ensuring environmental friendliness, and the compound plant disinfectant with complementary advantages, high efficiency and environmental protection is obtained.

A second object of the present invention is to provide a method for preparing the above composition for disinfectants, which is simple and convenient and is suitable for commercial promotion.

It is another object of the present invention to provide a specific use of the above composition for disinfectants.

In order to solve the above technical problems and achieve the above object, the present invention provides the following technical solutions:

in a first aspect, the present invention provides a composition for disinfectant, which consists of a Chinese medicinal composition for disrupting cell walls of gram-positive bacteria and a chemical disinfectant.

In an alternative embodiment, the gram positive cell wall disrupting traditional Chinese medicine composition comprises at least one of forsythia suspense leaf extract, emblica officinalis fruit extract, or zingiber corallinum hance extract.

In an alternative embodiment, the forsythia suspense leaf extract in the traditional Chinese medicine composition is an alcohol extract of forsythia suspense leaves, the emblic leafflower fruit extract is an alcohol extract of emblic leafflower fruits, and the coral ginger extract is coral ginger oil.

In an alternative embodiment, the oil contains 0.05-0.5 g of the alcoholic extract of forsythia suspense leaves and 0.05-0.5 g of the alcoholic extract of emblic leafflower fruits per ml of the zingiber corallinum oil.

In an alternative embodiment, the chemical sanitizing agent in the composition for a sanitizing agent comprises chlorine dioxide and/or polyhexamethylene guanidine hydrochloride.

In an alternative embodiment, the chemical disinfectant in the composition for disinfectant includes chlorine dioxide and polyhexamethylene guanidine hydrochloride, and the volume ratio of the traditional Chinese medicine composition, the chlorine dioxide and the polyhexamethylene guanidine hydrochloride in the composition for disinfectant is 6: (1-3): (1-3).

In an alternative embodiment, the composition for disinfectants further comprises an auxiliary material; the auxiliary material comprises a taste masking agent.

Preferably, the taste-masking agent comprises lavender essential oil and/or grapefruit essential oil.

Preferably, the volume ratio of the total volume of the traditional Chinese medicine composition and the chemical disinfectant to the taste masking agent is (100-500): 1.

in a second aspect, the present invention provides a method for preparing a composition for disinfectant as described in any one of the previous embodiments, wherein the method comprises adding a chemical disinfectant to a traditional Chinese medicine composition for destroying cell walls of gram-positive bacteria to obtain the composition for disinfectant.

Preferably, the gram-positive cell wall-disrupting traditional Chinese medicine composition comprises at least one of a forsythia suspense leaf extract, an emblic leafflower fruit extract or a zingiber corallinum hance extract.

Preferably, the forsythia suspense leaf extract and the emblic leafflower fruit extract are prepared by extracting forsythia suspense leaf and emblic leafflower fruit with ethanol, and drying to obtain the forsythia suspense leaf extract and the emblic leafflower fruit extract.

Preferably, the forsythia suspense leaves and the emblic leafflower fruits are crushed and then subjected to ethanol extraction.

Preferably, the forsythia suspense leaves and the emblic leafflower fruits are mixed and crushed, and then ethanol with the volume 4-6 times that of the crushed forsythia suspense leaves and the crushed emblic leafflower fruits is added for ethanol extraction.

Preferably, the Zingiber corallinum Hance extract is a nano Zingiber corallinum Hance oil, and the preparation method of the nano Zingiber corallinum Hance oil comprises the steps of uniformly mixing 40-50 parts of oleic acid, 0-1 part of stearic acid, 10-40 parts of polyethylene glycol, 0-15 parts of propylene glycol, 0-15 parts of ethylene glycol, 0-10 parts of anhydrous ethanol and 0-10 parts of distilled water to obtain a precursor solution; and (3) taking 100 parts of the obtained precursor solution, and uniformly mixing the precursor solution with 1-25 parts of terpinene-4-ol and 1-25 parts of Zingiber corallinum Hance oil to obtain the nanometer preparation type Zingiber corallinum Hance oil.

Preferably, the taste-masking agent is added after the combination of the herbal composition and the chemical disinfectant.

In a third aspect, the present invention provides a use of the composition for disinfectant according to any one of the preceding embodiments or the composition for disinfectant obtained by the production method according to the preceding embodiments in a disinfecting operation or in the production of a disinfected product.

In an alternative embodiment, the sterilization work includes a sterilization work in a residential area, a livestock farm, a public place, or a food processing place.

Preferably, the public place comprises a hospital or hotel.

The composition for the disinfectant comprises a traditional Chinese medicine composition for destroying cell walls of gram-positive bacteria and a chemical disinfectant, wherein in the disinfection process, the traditional Chinese medicine composition firstly destroys the cell walls of the gram-positive bacteria so that the chemical disinfectant can smoothly enter the bacteria to realize quick disinfection.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a graph showing the effect of the compositions for disinfectants according to example 1 and comparative example 3 of the present invention on the absorbance of Staphylococcus aureus;

fig. 2 is a result of zone of inhibition experiments for the composition for disinfectant, the chemical disinfectant and the Chinese medicinal composition provided in example 1 of the present invention;

FIG. 3 is an electron microscope image of Staphylococcus aureus after being exposed to a chemical disinfectant;

FIG. 4 is an electron microscope image of Staphylococcus aureus treated with the Chinese medicinal composition;

fig. 5 is an electron micrograph of staphylococcus aureus after being exposed to the composition for disinfectant provided in example 1.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In a specific embodiment, the present invention provides a composition for disinfectant, which consists of a Chinese medicinal composition for disrupting cell walls of gram-positive bacteria and a chemical disinfectant.

Optionally, the gram-positive cell wall-disrupting traditional Chinese medicine composition comprises at least one of a forsythia suspense leaf extract, an emblic leafflower fruit extract or a zingiber corallinum hance extract.

Fructus forsythiae has antibacterial, antiinflammatory, antiviral, antioxidant, endotoxin resisting, elastase activity inhibiting, phosphodiesterase inhibiting, heart tonifying, diuresis promoting, antipyretic, liver injury resisting, and hepatitis treating effects. Forsythia fruit, fructus forsythiae has many chemical components according to the eyesThe reports show that the extract mainly contains lignans, terpenes, volatile oils, flavonoids, coumarins, phenethyl alcohol and glycosides thereof, and the like. Wherein phillyrin is the main quality control index, and the content of phillyrin in the forsythia suspense medicinal materials is not less than 0.15%. Forsythiaside is lignan glycoside compound with chemical formula C₂₇H₃₄O₁₁The molecular weight is 534, the melting point is 181 ℃, and the nano-silver nano-particles are insoluble in water and soluble in organic solvents such as ethanol and methanol. A large number of research results prove that the chemical components in the forsythia suspense leaves have very good consistency with the chemical components in the forsythia suspense fruits, and the content of many chemical components in the forsythia suspense leaves is far higher than that of the forsythia suspense fruits, for example, the content of phillyrin in the forsythia suspense leaves is about 5%, the content of phillyrin in the forsythia suspense fruits is about 0.2%, and the content difference is over 20 times. The alcohol extract of forsythia suspense leaves is a nontoxic substance and has no mutagenic action, the active ingredients of forsythia suspense leaves include lignans (phillygenin), flavonoids (quercetin and rutin), triterpenes (ursolic acid), phillyrin, phillygenin, polyphenols, chlorogenic acid, (+) -pinoresinol, (-) -pinoresinol and the like, and phillyrin is reported to have strong antibacterial action and can inhibit the activity of cAMP phosphodiesterase.

Emblic leafflower fruit has different degrees of bacteriostasis to common pathogenic bacteria such as staphylococcus aureus, beta hemolytic streptococcus, salmonella typhi, pseudomonas aeruginosa, dysentery bacillus and the like, and has a wide antimicrobial spectrum. In addition, the compound has good inhibitory action on common food contamination bacteria such as bacillus subtilis and the like, has higher inhibitory activity on gram-positive bacteria than gram-negative bacteria, and is particularly suitable for large-scale special places such as hospitals, food processing plants and the like. In addition, animal model experiments prove that the phyllanthus emblica can protect the respiratory tract of the mouse against bacterial colonization after being fed for a long time, and methyl eugenol, beta-caryophyllene, beta-bylene and thymol in more than 30 chemical components of the phyllanthus emblica are main bacteriostatic components. And the effective components of the antibacterial action of the phyllanthus emblica leaf extract are concentrated at the CC part, and the phyllanthus emblica leaf extract has strong inhibiting effect on two clinically intractable serious drug-resistant strains, namely staphylococcus aureus drug-resistant strains and klebsiella pneumoniae. Moreover, the combination of Emblica officinalis with other herbs can also exert good antibacterial action, for example, TalwarGP, etc. has studied a compound herbal medicine Basant containing Emblica officinalis (herbal mixture containing Emblica officinalis extract, curcumin, Sapindus saponin and Aloe and Rose water), which is believed to have antibacterial action against bacterial gonococci causing genital infection, including penicillin, ciprofloxacin, tetracycline, and nalidixic acid resistant gonococcal strains, and has potential for treating the infection and promoting the recovery thereof.

Zingiber corallinum Hance, also known as YINJIANG, is the rhizome of Zingiber plant of Zingiberaceae, is a perennial herb, is mainly distributed in Yunnan, Guangxi and Guizhou provinces, is a traditional Chinese medicinal material for Miao ethnic group, and is often used as a medicine by coral ginger tubers for minority groups. It is bitter in taste and cold in nature. Has the functions of invigorating stomach, removing food retention, relieving swelling, removing toxic substance, and resisting bacteria, and has high medicinal value. The coral ginger is warm and pungent in smell and contains various volatile components, wherein the coral ginger oil serving as an effective component of the coral ginger has strong antibacterial property and is clinically used as an antibacterial medicament. In the effective components of the coral ginger, terpinene-4-ol is one of the main components, and a plurality of scholars at home and abroad verify that the effective components of the coral ginger, namely the terpinene-4-ol, have the effects of resisting bacteria, killing mites, resisting inflammation, resisting viruses and the like through research. Related documents report that in the antibacterial research of the effective component zingiber corallinum hance oil of zingiber corallinum hance and the effective component terpinen-4-ol, the zingiber corallinum hance and the zingiber strile are effective in inhibiting clinical isolated strains of staphylococcus aureus, escherichia coli, staphylococcus epidermidis, pseudomonas aeruginosa and klebsiella pneumoniae. Because the effective components of the zingiber corallinum hance oil and terpinene-4-ol are not easy to dissolve in water and have poor transdermal absorption, the pharmaceutical effect cannot be fully exerted, and the popularization and the application of the zingiber corallinum hance oil and the terpinene-4-ol are limited.

The folium forsythiae, fructus Phyllanthi and rhizoma Zingiberis Corallini can be extracted with water, ethanol or nanometer to obtain water extract, ethanol extract and nanometer extract.

Optionally, the forsythia suspense leaf extract in the traditional Chinese medicine composition is an alcohol extract of forsythia suspense leaves, the emblic leafflower fruit extract is an alcohol extract of emblic leafflower fruits, and the coral ginger extract is coral ginger oil.

Optionally, the zingiber corallinum hance oil is a nano-formulation zingiber corallinum hance oil.

Optionally, each milliliter of the nano-dosage form Zingiber corallinum Hance oil contains 0.05 to 0.5g of alcohol extract of forsythia suspense leaves and 0.05 to 0.5g of alcohol extract of emblic leafflower fruits.

Optionally, the chemical sanitizing agent in the composition for sanitizing agent includes chlorine dioxide and/or polyhexamethylene guanidine hydrochloride.

The action mechanism of chlorine dioxide is mainly oxidation, the electronic structure of the chlorine dioxide molecule is in an unsaturated state, the outer layer has 19 electrons, and the chlorine dioxide molecule has strong oxidation action force, and mainly attacks atomic groups (such as enzyme containing sulfydryl, sulfide and chloride) rich in electrons (or electron donors) to rob electrons forcibly, so that the chlorine dioxide molecule becomes a substance losing activity and changing properties, and the aim is achieved. The chlorine dioxide releases atomic oxygen to oxidize the enzyme containing sulfhydryl in the cell to play the role of sterilization. Chlorine dioxide is deodorized because it can react with off-flavor substances (e.g., H)₂S、-SOH、-NH₂Etc.) to undergo dehydration reaction and to rapidly oxidize and convert the foreign flavor substances into other substances. It also prevents the decomposition of methionine into ethylene and also destroys the ethylene which has formed, thus delaying decay, while killing microorganisms without reacting with fatty acids and without destroying the structure of the food.

Polyhexamethylene guanidine hydrochloride (international common name: PHMG) is a safe, efficient and broad-spectrum high-molecular antimicrobial agent, and is widely applied to the fields of sanitation and disinfection, water treatment, sanitary products, textile, plastics, aquaculture, agricultural planting and the like. It is a semitransparent amorphous resin-like solid, has no special smell, is easily dissolved in water, and has no color of water solution to light yellow, pH close to neutral, and no corrosion to metal. The molecule is high polymer, is not easy to be absorbed by animal tissues, and has low biological toxicity. Has no irritation to skin, no carcinogenesis, no mutagenicity, and no gonadotropic effect. The antibacterial action mode and mechanism are as follows: guanidine has high activity, can generate ionization in aqueous solution, has strong electropositivity on a hydrophilic group part, is easy to adsorb various bacteria and viruses with electronegativity, enters a cell membrane, inhibits the synthesis of intramembrane liposome to cause cell apoptosis, and simultaneously blocks a breathing channel of a microorganism by a membrane formed by a polymer to achieve a sterilization effect. It forms a thin sterilizing film on the surface of the sterilized object, and can be used together with other disinfectants.

According to the information, the forsythia suspense leaf, emblic leafflower fruit and zingiber corallinum hance extract have a wide bactericidal spectrum, and the compounds in the forsythia suspense leaf, emblic leafflower fruit and zingiber corallinum hance extract have the best bactericidal effect on gram-positive bacteria such as staphylococcus aureus, in addition, chemical extracts in the forsythia suspense leaf, emblic leafflower fruit and zingiber corallinum hance extract also have a certain drug resistance to severe drug-resistant staphylococcus aureus and klebsiella pneumoniae, and terpinen-4-ol and zingiber corallinum hance oil extracted from zingiber corallinum hance also show good antibacterial performance on drug-resistant bacteria through k-b method tests after nanocrystallization. The cell wall of the gram-positive bacteria consists of a glycan skeleton, a pentapeptide cross-linking bridge and a tetrapeptide side chain, and the gram-negative bacteria lack the pentapeptide cross-linking bridge, so the cell wall of the gram-positive bacteria is strong in toughness, the cell wall of the gram-negative bacteria is weak in toughness, the gram-positive bacteria, the cell wall of the gram-negative bacteria and the cell wall of the gram-negative bacteria are compounded in a certain mode, the gram-positive bacteria and the cell wall of the gram-negative bacteria can be synergistically enhanced, and particularly the wall breaking effect of the gram-positive bacteria with thicker cell walls is more obvious, so that chlorine dioxide and polyhexamethylene guanidine hydrochloride can easily enter the interior of the bacteria, and the sterilization effect is greatly enhanced, but not a simple additive effect. And chlorine dioxide is prepared by the action of sodium chlorate, sulfuric acid and methanol or the action of sodium chlorate and sulfur dioxide, low-concentration chlorine dioxide has mixed smell of grass and soil, has irritant smell similar to chlorine gas at high concentration, has strong irritability, mainly causes respiratory tract irritation after contacting, can cause pulmonary edema when being inhaled at high concentration, can kill the disease, can cause serious damage to the respiratory tract, and has irritability to skin due to high-concentration gas. The skin can be exposed to or ingested with the product in high concentration, which can cause strong irritation and corrosion, and chronic bronchitis due to long-term exposure to high concentration. The chemical components of the emblic leafflower fruit, such as methyl eugenol, beta-caryophyllene, beta-bylene, thymol and the like, can protect the respiratory tract of people, resist bacterial colonization and certain chemical stimulation, and can reduce and even offset the adverse effect of chlorine dioxide and polyhexamethylene guanidine hydrochloride disinfectant on human bodies to the maximum extent.

Optionally, the chemical disinfectant in the composition for disinfectant comprises chlorine dioxide and polyhexamethylene guanidine hydrochloride, and the volume ratio of the traditional Chinese medicine composition to the chlorine dioxide to the polyhexamethylene guanidine hydrochloride in the composition for disinfectant is 6: (1-3): (1-3).

Optionally, the composition for the disinfectant also contains auxiliary materials; the auxiliary material comprises a taste masking agent;

preferably, the taste-masking agent comprises lavender essential oil and/or grapefruit essential oil;

the added chlorine dioxide chemical disinfectant is smelly, irritates human bodies and is not suitable for food catering places, so that the lavender essential oil and/or the grapefruit essential oil are added into the composite disinfectant, wherein the lavender essential oil can be used for killing insects and cleaning wounds, and when world war antibiotics are lacked, the lavender essential oil is used for replacing anti-inflammation, a small amount of lavender essential oil can be added into vegetables to remove flatulence and increase flavor, Roman often uses incense to remove bacteria in the air, and simultaneously, the chlorine dioxide chemical disinfectant can relieve the pain caused by headache and related nervous tension and relieve anxiety, and is particularly suitable for public places such as hospitals; the grapefruit essential oil is obtained by squeezing pericarp, has fresh and sweet smell, has citrus fruit fragrance, has the effects of promoting body fluid discharge, promoting urination and detoxifying, has the effects of losing weight and soothing nerves, can relieve symptoms such as headache and fatigue, has a certain promotion effect on bile secretion, and can help to digest fat. In addition, the medicine has the effects of resisting bacteria and diminishing inflammation, can regulate oily and pimpled skins, can help the kidney to expel toxin and can prevent the occurrence of kidney stones. It also has light fragrance, and can relieve stress.

Therefore, the green natural disinfectant formed by mixing the forsythia suspense leaves, the zingiber corallinum hance, the emblic leafflower fruit, the chlorine dioxide, the polyhexamethylene biguanide hydrochloride, the lavender essential oil and the grapefruit essential oil can be applied to disinfection and sterilization of commercial and industrial scenes such as markets, public places and the like, can also be applied to disinfection of residential areas, manufacturing or processing units, animal husbandry and breeding, can completely meet the requirements of disinfection and sterilization of daily work and living environment, and provides powerful guarantee for healthy life.

In another embodiment, the present invention provides a method for preparing the composition for disinfectant as set forth in any one of the preceding embodiments, wherein the method comprises adding a chemical disinfectant to the traditional Chinese medicine composition for gram-positive bacteria cell wall disruption to obtain the composition for disinfectant.

Preferably, the gram-positive bacteria cell wall-disrupting traditional Chinese medicine composition comprises at least one of forsythia suspense leaf extract, emblica officinalis fruit extract or zingiber corallinum hance extract.

Preferably, the Zingiber corallinum Hance extract is a nano Zingiber corallinum Hance oil, and the preparation method of the nano Zingiber corallinum Hance oil comprises the steps of uniformly mixing 40-50 parts of oleic acid, 0-1 part of stearic acid, 10-40 parts of polyethylene glycol, 0-15 parts of propylene glycol, 0-15 parts of ethylene glycol, 0-10 parts of anhydrous ethanol and 0-10 parts of distilled water to obtain a precursor solution; respectively taking 100 parts of the obtained precursor solution, and uniformly mixing the precursor solution with 1-25 parts of terpinene-4-ol and 1-25 parts of Zingiber corallinum Hance oil to obtain the nanometer preparation type Zingiber corallinum Hance oil.

In another embodiment, the present invention provides a use of the composition for disinfectant according to any one of the preceding embodiments or the composition for disinfectant obtained by the method for preparing the preceding embodiments in a disinfecting operation or in the preparation of a disinfecting product.

Preferably, the public place comprises a hospital or hotel.

Some embodiments of the invention are described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.

Example 1

The embodiment provides a composition for a disinfectant, which consists of a traditional Chinese medicine composition, chlorine dioxide, polyhexamethylene guanidine hydrochloride and a taste masking agent, wherein the volume ratio of the traditional Chinese medicine composition to the chlorine dioxide to the polyhexamethylene guanidine hydrochloride is 3:1:1, the volume ratio of the total volume of the traditional Chinese medicine composition, the chlorine dioxide and the polyhexamethylene guanidine hydrochloride to the taste masking agent is 500:1, and the taste masking agent consists of lavender essential oil and grapefruit essential oil in the volume ratio of 1: 1. The traditional Chinese medicine composition is a nano-dosage form of coral ginger oil containing 10g of alcohol extract of forsythia suspense leaves and 10g of alcohol extract of emblic leafflower fruits, the total volume is 100ml, and the coral ginger oil further comprises 40 parts of oleic acid, 1 part of stearic acid, 19 parts of polyethylene glycol, 10 parts of propylene glycol, 10 parts of ethylene glycol, 10 parts of absolute ethyl alcohol, 10 parts of distilled water, 25 parts of terpinene-4-alcohol and 25 parts of coral ginger oil according to parts by weight.

The preparation method of the ethanol extract of the forsythia suspense leaves and the ethanol extract of the emblic leafflower fruits comprises the steps of mixing the forsythia suspense leaves and the emblic leafflower fruits, crushing, adding 5 times of ethanol for ethanol extraction, and volatilizing the ethanol to obtain the corresponding ethanol extract.

The preparation method of the nano-dosage form Zingiber corallinum Hance oil comprises the steps of uniformly mixing 40 parts of oleic acid, 1 part of stearic acid, 19 parts of polyethylene glycol, 10 parts of propylene glycol, 10 parts of ethylene glycol, 10 parts of absolute ethyl alcohol and 10 parts of distilled water to obtain a precursor solution; and uniformly mixing the precursor solution with 25 parts of terpinene-4-ol and 25 parts of Zingiber corallinum Hance oil to obtain the nanometer Zingiber corallinum Hance oil.

Example 2

The present example provides a composition for disinfectant, which is different from example 1 in the amount of only a part of components, and comprises a herbal composition, chlorine dioxide, polyhexamethylene guanidine hydrochloride, and a taste-masking agent, wherein the volume ratio of the herbal composition, chlorine dioxide, and polyhexamethylene guanidine hydrochloride is 3:1:1, the volume ratio of the total volume of the traditional Chinese medicine composition, the chlorine dioxide and the polyhexamethylene guanidine hydrochloride to the taste masking agent is 500:1, and the taste masking agent consists of lavender essential oil and grapefruit essential oil in the volume ratio of 1: 1. The traditional Chinese medicine composition is a nano-dosage form of coral ginger oil containing 5g of an alcohol extract of forsythia suspense leaves and 5g of an alcohol extract of emblic leafflower fruits, the total volume is 100ml, and the coral ginger oil further comprises 40 parts by weight of oleic acid, 10 parts by weight of polyethylene glycol, 10 parts by weight of propylene glycol, 10 parts by weight of ethylene glycol, 10 parts by weight of absolute ethyl alcohol, 10 parts by weight of distilled water, 5 parts by weight of terpinene-4-ol and 5 parts by weight of coral ginger oil.

Example 3

The present example provides a composition for disinfectant, which is different from example 1 in the amount of only a part of components, and comprises a herbal composition, chlorine dioxide, polyhexamethylene guanidine hydrochloride, and a taste-masking agent, wherein the volume ratio of the herbal composition, chlorine dioxide, and polyhexamethylene guanidine hydrochloride is 3:1:1, the volume ratio of the total volume of the traditional Chinese medicine composition, the chlorine dioxide and the polyhexamethylene guanidine hydrochloride to the taste-masking agent is 500:1, and the volume ratio of the taste-masking agent is 1:1 lavender essential oil and grapefruit essential oil. The traditional Chinese medicine composition is a nano-dosage form of coral ginger oil containing 50g of alcohol extract of forsythia suspense leaves and 50g of alcohol extract of emblic leafflower fruits, the total volume is 100ml, and the coral ginger oil comprises 45 parts of oleic acid, 10 parts of polyethylene glycol, 5 parts of propylene glycol, 5 parts of ethylene glycol, 5 parts of absolute ethyl alcohol, 10 parts of distilled water, 10 parts of terpinene-4-alcohol and 10 parts of coral ginger oil according to parts by weight.

Example 4

This example provides a specific method of preparing the composition for disinfectants described in example 1, comprising the steps of:

(1) cleaning folium forsythiae and fructus Phyllanthi with water, drying, pulverizing with ultrasonic grinder, mixing, soaking in anhydrous ethanol solution for 48 hr to obtain soaking solution, wherein the volume ratio of the mixture to anhydrous ethanol is 1:5, filtering with filter paper, and evaporating off anhydrous ethanol from the filtrate with rotary evaporator to obtain ethanol extract of folium forsythiae and fructus Phyllanthi.

(2) Firstly, uniformly mixing oleic acid, stearic acid, polyethylene glycol, propylene glycol, ethylene glycol, absolute ethyl alcohol and distilled water, reacting and stirring for 30-60 minutes at the temperature of 30-50 ℃ through an ultrasonic wave or an emulsifying machine, standing, and cooling until the solution is clear and transparent to obtain a precursor solution; and (3) uniformly mixing the precursor solution with terpinene-4-ol and Zingiber corallinum Hance oil by ultrasonic wave to obtain the nanometer Zingiber corallinum Hance oil.

(3) And (3) adding the alcohol extracts of the forsythia suspense leaves and the emblic leafflower fruits obtained in the step (1) into the nano-dosage form zingiber corallinum hance oil obtained in the step (2) to obtain the traditional Chinese medicine composition.

(4) Adding two chemical disinfectants, namely chlorine dioxide and polyhexamethylene biguanide hydrochloride, into the traditional Chinese medicine composition obtained in the step (3), wherein the volume ratio of the chlorine dioxide to the polyhexamethylene biguanide hydrochloride is 3:1: 1.

(5) And (3) adding equal volumes of lavender essential oil and grapefruit essential oil into the mixture obtained in the step (4), wherein the volume ratio of the disinfectant to the two essential oils is 500:1, and obtaining the final composition for the disinfectant.

Comparative example 1

This comparative example provides a composition for disinfectant, to which alcohol extract of forsythia suspense leaves was not added, compared to example 1.

Comparative example 2

This comparative example provides a composition for disinfectant, to which alcohol extract of emblic leafflower fruit is not added, compared to example 1.

Comparative example 3

This comparative example provides a composition for a disinfectant, which does not incorporate nano-dosage forms of Zingiber corallinum Hance oil, as compared to example 1.

Comparative example 4

This comparative example provides a composition for a disinfectant, without the addition of chlorine dioxide, compared to example 1.

Comparative example 5

This comparative example provides a composition for a disinfectant, in which polyhexamethylene guanidine hydrochloride was not added, compared to example 1.

Comparative example 6

This comparative example provides a composition for disinfectant, in which the alcohol extract of forsythia suspense leaves was replaced with the alcohol extract of cinnamon, as in example 1, in the same manner as the alcohol extract of forsythia suspense leaves in example 4.

Comparative example 7

This comparative example provides a composition for disinfectant, in which the alcohol extract of the emblic leafflower fruit was replaced with the alcohol extract of the leaves of the emblic leafflower fruit, as compared with example 1, in the same manner as the alcohol extract of the emblic leafflower fruit of example 4.

Comparative example 8

This comparative example provides a composition for a disinfectant, which compared to example 1, replaced the nano-formulation zingiber corallinum oil with a common commercially available non-nano zingiber corallinum oil.

Examples of effects

1. Evaluation of Sterilization Effect

According to the method specified in the disinfectant laboratory sterilization effect test method (2020 edition), the sterilization and disinfection effects of the prepared compound plant disinfectant are detected, and the sterilization and disinfection effects of the composition for the disinfectant provided in examples 1 to 3 and comparative examples 1 to 8 are observed by using a suspension quantitative sterilization test.

Experimental strains: staphylococcus aureus standard strain (purchased from China center for microbiological Collection, strain ATCC 25923), and Staphylococcus aureus resistant strain.

The method comprises the following steps: test suspensions were prepared to a concentration of 1X 10⁸cfu/mL. Taking a large sterile test tube, sequentially adding 0.5mL of test bacterial suspension, 0.5mL of organic interference substance and 4.0mL of disinfectant, and rapidly mixing uniformly; after the experimental bacteria and the disinfectant interact for a certain time, removing the residual disinfectant by using a filtration flushing method, finally, pasting a filter membrane (with the aperture of 0.45um) with the bacterial surface facing upwards on the surface of a nutrient agar culture medium flat plate, placing the nutrient agar culture medium flat plate in an incubator at 37 ℃ for culturing for 48 hours, then measuring the bacterial colony number, replacing the disinfectant with standard hard water, and using 1After 0-fold dilution, the residual disinfectant was removed by filtration and washing, and the viable bacteria concentration was measured as a positive control. The experiment was repeated 3 times, the viable bacteria concentration (cfu/mL) of each group was calculated and converted to a log (N), and then the log kill value (KL) was calculated as the log of the average viable bacteria concentration of the control group (No) -the log of the viable bacteria concentration of the experimental group (Nx).

Evaluation indexes are as follows: the log kill values for the coliform group and golden staphylococcus bacteria were greater than or equal to 5 according to the national food safety standard disinfectant (GB14930.2-2012), and the results are shown in table 1.

TABLE 1 Bactericidal Effect of the compositions for disinfectants provided in examples 1 to 3 and comparative examples 1 to 8

Detailed description of the preferred embodiments	Sterilizing effect
		Example 1	99.99％
Example 2	99.99％
		Example 3	99.99％
Comparative example 1	68.99％
		Comparative example 2	81.25％
Comparative example 3	68.11％
		Comparative example 4	77.53％
Comparative example 5	71.69％
		Comparative example 6	51.67％
Comparative example 7	52.00％
		Comparative example 8	76.23％

2. Evaluation of stability

2.1 high speed centrifugation test

Taking a proper amount of the composition for the disinfectant, putting the composition into a 2mL centrifuge tube, sealing the tube opening, putting the centrifuge tube into a high-speed centrifuge, centrifuging the centrifuge tube at 14000r/min for 10min, and observing whether the phenomena of precipitation, turbidity, delamination and the like occur.

2.2 temperature test

40mL of the composition for a disinfectant was dispensed into 4 brown glass bottles, which were sealed and placed in a-4 ℃ refrigerator and a 25 ℃, 37 ℃, 60 ℃, 80 ℃, 100 ℃ water bath cabinet, respectively, for 1 day, and the appearance was observed to see if there was a change.

2.3 light test

The compound disinfectant solution is sealed in a transparent glass bottle, placed for 7 days under the irradiation of a 20w fluorescent lamp, and the appearance change is observed on the 1 st, 3 rd, 5 th and 7 th days respectively.

As a result: through high-speed centrifugal test and temperature and light stability test investigation, the composition for the disinfectant provided by the embodiment 1-3 is still clear and transparent, no phenomena such as precipitation, turbidity, layering and precipitation occur, and the solution has no change of properties and color, which shows that the stability is good.

2.4 according to the corresponding method specified in the technical specification for disinfection (2002), the compositions for disinfectants provided in examples 1-3 and comparative example 3 were placed in a constant temperature incubator at 37 ℃ (relative humidity > 75%) and stored for 90 days, the bactericidal effect of the compound disinfectant on the test strains was tested, and the test was repeated 3 times, with the results shown in table 2.

TABLE 2 Long-acting germicidal Effect of the compositions for disinfectants provided in examples 1 to 3 and comparative example 3

Evaluation indexes are as follows: the killing logarithm value of the staphylococcus aureus and the escherichia coli group is more than or equal to 5 according to the standard of a national food safety standard disinfectant (GB 14930.2-2012).

As a result: the compound disinfectant has the effect of sterilizing standard staphylococcus aureus strains (ATCC 25923) for more than 99.9 percent in 2 minutes, and meets the national standard.

3. Composition for disinfectant having effect on cell wall of Staphylococcus aureus

The main component of the bacterial cell wall is peptidoglycan, so that the nonionic surfactant triton x-100 has complete permeability to the plasma membrane of the bacterial cell, so that it was selected as a positive control, and the ability of the composition for disinfectant provided in example 1 and comparative example 3 of the present invention to act on the cell wall was judged using 1 × PBS buffer as a negative control, whenever the peptidoglycan structure could be broken or the substance inhibiting its synthesis could damage the cell wall. As shown in fig. 1, after TnitonX-100 acts on bacteria, the concentration of the bacteria is reduced from the initial OD630 ═ 0.058 to 0.031 after 3 hours, which shows that the bacteria have obvious destructive effect on the bacterial cell walls; as can be seen from the results of the disinfectant group in example 1, the destructive effect on cell walls is stronger within 30min than that of a positive control group, the disinfectant is gradually stable after 30min of action, and the action capacity is equivalent to 70.4% of that of TritonX-100 within 3h, which indicates that the disinfectant has better destructive effect on the cell walls of staphylococcus aureus.

By adopting a zone of inhibition experiment, the bacteriostatic effect of the traditional Chinese medicine composition used in example 1 on staphylococcus aureus (ATCC 25923) is examined, chlorine dioxide and polyhexamethylene guanidine hydrochloride in an amount equal to that in example 1 are used as a chemical disinfectant, the bacteriostatic effect of the traditional Chinese medicine composition and the chemical disinfectant on staphylococcus aureus (ATCC 25923) is examined, and the bacteriostatic effects of the traditional Chinese medicine composition and the chemical disinfectant are compared with that in example 1, as shown in fig. 2, it can be seen that the composition for disinfectant, the chemical disinfectant and the traditional Chinese medicine composition provided in example 1 of the present invention all have bacteriostatic effects on staphylococcus aureus (ATCC 25923), wherein the zone of inhibition generated by the composition for disinfectant provided in example 1 is the largest, and therefore, the bactericidal effect is the strongest.

The interaction between the three bactericides and staphylococcus aureus is observed by using a field emission scanning electron microscope, the result is shown in figures 3-5, and in order to better reflect the bacteriostasis of the three bactericides, a region where live bacteria and inactivated bacteria coexist is selected for shooting. Wherein fig. 3 is an interaction result of the chemical disinfectant with staphylococcus aureus, fig. 4 is an interaction result of the traditional Chinese medicine composition with staphylococcus aureus, and fig. 5 is an interaction result of the composition for disinfectant provided in example 1 with staphylococcus aureus. In FIGS. 3 to 5, the left image is an electron micrograph at magnification of 40000 times, and the right image is a scanning electron micrograph at magnification of 20000 times.

As can be seen from fig. 3, the inactivated staphylococcus aureus still has a complete cell wall contour, which proves that the chemical disinfectant does not damage the cell wall, but directly permeates the membrane to realize sterilization, while as can be seen from fig. 4, under the action of the traditional Chinese medicine composition, the cell wall of the staphylococcus aureus is damaged in a large area, independent thalli cannot be distinguished, but a large amount of residues are remained in the thalli with the damaged cell wall, and as can be seen from a visual point of view in fig. 5, the cell wall of the staphylococcus aureus is basically damaged in the inactivation area, and compared with fig. 4, the residues of the inactivated thalli are significantly reduced.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition for a disinfectant, which consists of a traditional Chinese medicine composition for destroying cell walls of gram-positive bacteria and a chemical disinfectant.

2. The disinfectant composition according to claim 1, wherein said cell wall-disrupting herbal composition comprises at least one of forsythia suspensa leaf extract, emblica officinalis fruit extract, or zingiber corallinum hance extract.

3. The composition for disinfectant according to claim 2, wherein the forsythia suspense leaf extract in the traditional Chinese medicine composition is an alcohol extract of forsythia suspense leaves, the emblic leafflower fruit extract is an alcohol extract of emblic leafflower fruits, and the coral ginger extract is coral ginger oil;

preferably, the Zingiber corallinum Hance oil is nanometer Zingiber corallinum Hance oil.

4. The composition for disinfectant as set forth in claim 3, wherein the alcoholic extract of forsythia suspense leaves is contained in an amount of 0.05-0.5 g and the alcoholic extract of emblic leafflower fruits is contained in an amount of 0.05-0.5 g per ml of the nano-formulation zingiber corallinum oil.

5. A composition for disinfectants according to claim 1, wherein the chemical disinfectant in the composition for disinfectants comprises chlorine dioxide and/or polyhexamethylene guanidine hydrochloride.

6. The composition for disinfectant, according to claim 5, wherein said chemical disinfectant in said composition for disinfectant comprises chlorine dioxide and polyhexamethylene guanidine hydrochloride, and said composition for disinfectant has a volume ratio of the traditional Chinese medicine composition, chlorine dioxide and polyhexamethylene guanidine hydrochloride of 6: (1-3): (1-3).

7. The composition for disinfectant according to any one of claims 1 to 6, further comprising an auxiliary material; the auxiliary material comprises a taste masking agent;

8. the method for preparing a composition for disinfectant according to any one of claims 1 to 7, wherein the method for preparing the composition for disinfectant comprises adding a chemical disinfectant to a Chinese medicinal composition for destroying cell walls of gram-positive bacteria to obtain the composition for disinfectant;

preferably, the gram-positive cell wall-disrupting traditional Chinese medicine composition comprises at least one of a forsythia suspense leaf extract, an emblic leafflower fruit extract or a zingiber corallinum hance extract;

preferably, the forsythia suspense leaf extract and the emblic leafflower fruit extract are prepared by extracting forsythia suspense leaf and emblic leafflower fruit with ethanol, and drying to obtain a forsythia suspense leaf extract and an emblic leafflower fruit extract;

preferably, the forsythia suspense leaves and the emblic leafflower fruits are subjected to ethanol extraction after being crushed;

preferably, the forsythia suspense leaves and the emblic leafflower fruits are mixed and crushed, and then ethanol with the volume 4-6 times that of the forsythia suspense leaves and the emblic leafflower fruits is added for ethanol extraction;

preferably, the Zingiber corallinum Hance extract is a nano Zingiber corallinum Hance oil, and the preparation method of the nano Zingiber corallinum Hance oil comprises the steps of uniformly mixing 40-50 parts of oleic acid, 0-1 part of stearic acid, 10-40 parts of polyethylene glycol, 0-15 parts of propylene glycol, 0-15 parts of ethylene glycol, 0-10 parts of anhydrous ethanol and 0-10 parts of distilled water to obtain a precursor solution; taking 100 parts of the obtained precursor solution, and uniformly mixing the precursor solution with 1-25 parts of terpinen-4-ol and 1-25 parts of Zingiber corallinum Hance oil to obtain nanometer preparation type Zingiber corallinum Hance oil;

9. Use of the composition for disinfectant according to any one of claims 1 to 7 or the composition for disinfectant obtained by the preparation method according to claim 8 in a disinfection operation or in the preparation of a disinfectant product.

10. Use according to claim 9, wherein the sterilization work comprises sterilization work in residential areas, animal husbandry places, public places or food processing places;

preferably, the public place comprises a hospital or hotel.