CN114532349B - Compound pesticide containing spinosad and hexythiazox and application thereof - Google Patents
Compound pesticide containing spinosad and hexythiazox and application thereof Download PDFInfo
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- CN114532349B CN114532349B CN202210276870.6A CN202210276870A CN114532349B CN 114532349 B CN114532349 B CN 114532349B CN 202210276870 A CN202210276870 A CN 202210276870A CN 114532349 B CN114532349 B CN 114532349B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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Abstract
The invention discloses a compound pesticide containing spinosad and hexythiazox and application thereof, wherein the effective components of the composition comprise spinosad and hexythiazox, and the mass ratio of the spinosad to the hexythiazox is (0.1-10): 1. According to the invention, after the spinosad and the hexythiazox are compounded, the insecticidal composition has a certain synergism and an insecticidal spectrum-enlarging activity on the tetranychus, can obviously improve the control effect, reduce the pesticide usage amount, reduce the use cost and reduce the environmental pollution.
Description
Technical Field
The invention belongs to the technical field of pest control, and in particular relates to a compound pesticide with synergistic activity and an expanded insecticidal spectrum for controlling spider mites and application thereof.
Background
Tetranychus cinnabarinus is also called red spider, belongs to Tetranychus spider mite of Acarina, is widely distributed in various regions of China, and is harmful to various solanaceous and flower crops such as eggplants, vegetable peppers, tomatoes, cucumbers, china rose and the like. Generally, the weight of the plant is heavier in summer, and the plant can be seriously damaged in a short time and a large area. The chemical control is a main control method of tetranychus cinnabarinus, but the chemical pesticide is used for a long time, she Manyi generates drug resistance, so that the control effect is reduced, and the ecological environment is destroyed and the human health is threatened by frequently using the chemical pesticide, so that the control of harmful insects and mites is attracting more attention. The pesticide is effective to one or a few insect pests, and has the defect of narrow insecticidal spectrum, so that in practical production, different pesticide varieties or mixed synergism and expanded insecticidal spectrum between biological pesticide varieties and chemical pesticide varieties are adopted in order to delay the drug resistance of the insect pests, expand the insecticidal spectrum and improve the drug effect. The method for judging whether the two medicaments are synergistic, antagonistic or additive or not by mixing can be judged by taking a certain pest or mite as a test object, measuring the toxicity of the two medicaments indoors, screening the optimal proportion of the two medicaments and solving the co-toxicity coefficient of the two medicaments, if the co-toxicity coefficient after compounding is more than 100, the synergistic effect is obtained, and the compound synergistic mixture can further verify the efficacy of the two medicaments by potting and field efficacy. The synergistic compound agent improves the actual control effect, reduces the using amount of pesticides, delays the generation of drug resistance of pests and expands the insecticidal spectrum, thereby being an important means for the comprehensive control of the pests.
The spinosad is a macrolide nuisance-free high-efficiency biological pesticide extracted from fermentation liquor of spinosad sugarcane and polyporus (Saccharopolyspora spinosa), has rapid contact killing and stomach poisoning effects on pests, has strong penetration effect on leaves, can kill pests below epidermis, has long residual effect period and has a certain ovicidal effect on some pests. Has no systemic effect and can effectively prevent and treat lepidoptera, diptera and thysanoptera pests.
The hexythiazox is called as Nixolone, and has the characteristics of killing mites: has strong contact killing and stomach poisoning effects on mites; the plant leaf has stronger penetrating effect on the epidermis of crops, can penetrate into leaves and penetrate to leaf backs to kill harmful mites on the leaf backs, but has no systemic effect; effective against mite eggs, if mites and young mites, and little virulence against adult mites, however, the female adult mites contacted with the agent have low spawning hatchability; the drug effect is slow to play, and the peak of the drug effect is reached generally 7-10 days after the drug administration, and the lasting period is 40-50 days; no significant difference in effect at high or low temperature; the composition is used at common concentration, and has little influence on crops, natural enemies, bees and predatory mites. The hexythiazox can prevent and treat spider mites on various crops such as fruits, forests, tea, cotton, vegetables, all beans, flowers and the like, but has poor rust mite prevention effect.
The spinosad and the hexythiazox have a lot of researches and reports on the biological activity of the tetranychus, but the spinosad is mainly used for controlling the adult mites, and has quick acting and good effect, the hexythiazox has good effect on the adult mites and young mites, and has low toxicity on the adult mites, and the drug effect is slow, but the report on the synergistic effect and the compounding proportion of the spinosad and the hexythiazox is irrelevant, so that the mixed pesticide of the spinosad and the hexythiazox with synergistic activity is designed for solving the problems that the adult mites cannot take account of each other and the drug effect is unstable when the spinosad and the hexythiazox are singly used.
Disclosure of Invention
The invention aims to: the invention aims to solve the technical problem of providing a composition aiming at the defects of the prior art.
The invention also provides an insecticide containing the composition.
The invention also solves the technical problem of providing the application of the composition in preparing pesticides.
In order to solve the first technical problem, the invention discloses a composition, and the effective components of the composition comprise spinosad and hexythiazox.
Wherein the mass ratio of the spinosad to the hexythiazox is (0.1-10): 1; in some embodiments, the mass ratio of spinosad to hexythiazox is (3.7-6.1): 1; in some embodiments, the mass ratio of spinosad to hexythiazox is (4.2-5.6): 1; in some embodiments, the mass ratio of spinosad to hexythiazox is 4.2:1 or 5.6:1.
In order to solve the second technical problem, the invention discloses an insecticide.
Wherein the pesticide comprises spinosad and hexythiazox; in some embodiments, the mass ratio of spinosad to hexythiazox is (0.1-10): 1; in some embodiments, the mass ratio of spinosad to hexythiazox is (3.7-6.1): 1; in some embodiments, the mass ratio of spinosad to hexythiazox is (4.2-5.6): 1; in some embodiments, the mass ratio of spinosad to hexythiazox is 4.2:1 or 5.6:1.
In some embodiments, the controlling spider mites is controlling spider mites in vegetables and/or flowers.
In order to solve the third technical problem, the invention discloses application of a composition in preparing pesticides.
Wherein the active ingredients of the composition comprise spinosad and hexythiazox; in some embodiments, the mass ratio of spinosad to hexythiazox is (0.1-10): 1; in some embodiments, the mass ratio of spinosad to hexythiazox is (3.7-6.1): 1; in some embodiments, the mass ratio of spinosad to hexythiazox is (4.2-5.6): 1; in some embodiments, the mass ratio of spinosad to hexythiazox is 4.2:1 or 5.6:1.
Wherein the pesticide is a pesticide for preventing and controlling spider mites.
In some embodiments, the controlling spider mites is controlling spider mites in vegetables and/or flowers.
The spinosad and the hexythiazox used in the invention can be directly purchased from the market, and at present, a plurality of companies produce the drug in the market, and the source is wide, so long as the proportion range and the application condition of the invention are met.
The beneficial effects are that: compared with the prior art, the invention has the following advantages:
according to the invention, after the spinosad and the hexythiazox are compounded, the insecticidal composition has a certain synergism and an expanded insecticidal spectrum activity on the tetranychus in a certain compounding proportion, can obviously improve the control effect, reduce the use amount of pesticides, reduce the use cost and reduce the environmental pollution.
Detailed Description
The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials, unless otherwise specified, are commercially available.
The following is a detailed description of the synergistic combination of spinosad and hexythiazox, which is provided for illustration only and is not meant to limit the scope of the present invention, wherein the active ingredients are calculated as active ingredients.
Experimental example 1: compound proportion screening and co-toxicity coefficient determination of spinosad and hexythiazox for tetranychus cinnabarinus
1.1 test pests and test methods
Test insects: tetranychus cinnabarinus propagated by eggplant seedlings at school and gardening stations.
1.2 reagents for test
30% spinosyn suspension (Tao Shiyi farm, U.S.); 5% hexythiazox emulsifiable concentrate (Jiangsu Longda lamp chemical Co., ltd.); spinosad and hexythiazox admixture: is prepared from single dosage according to proportioning requirements.
1.3 Experimental methods
1.3.1 determination of the virulence of spinosad and hexythiazox on Tetranychus cinnabarinus
The experiment adopts a leaf dipping method. Based on the pretreatment test results, the formulations (spinosad and hexythiazox) were formulated and diluted sequentially to 5 series of concentration gradients (200 ppm,100ppm,50ppm,25ppm,12.5 ppm). Immersing the collected eggplant leaves with the back clustered spider mites (20-25 heads) into the liquid medicine, standing the leaves in the liquid medicine for 40 seconds, taking out the leaves with forceps, placing the leaves in a clean culture dish, airing the leaves at normal temperature, and wrapping absorbent cotton at the petioles of the leaves for moisturizing treatment. Placing the spider mites into a culture dish added with water-absorbing filter paper for storage, sealing the spider mites by using a sealing film, testing the spider mites under the environment conditions that the temperature is 20-25 ℃, the light and dark period is 10-14 h and the relative humidity is 75-85%, checking the death (living) insect population (touching an insect body with a writing brush tip under an dissecting mirror and judging whether the spider mites survive) at 48h, 72h and 120h respectively, and recording, sorting and calculating the death rate of the insect population of the spider mites and correcting the death rate. The data are summarized on Excel software, and the virulence base line, the correlation coefficient, the medium death amount and the like of each medicament on tetranychus cinnabarinus are calculated.
1.3.2 screening of synergistic Agents of different combinations of spinosad and hexythiazox
According to the toxicity baseline of 2 acaricides measured in test 1.3.1 on tetranychus cinnabarinus, LC is calculated respectively 50 Value according to LC 50 Preparing 70mg/L spinosad aqueous solution and 50mg/L hexythiazox aqueous solution, preparing 7 mixed solutions with different volume ratios from the 70mg/L spinosad aqueous solution and the 50mg/L hexythiazox aqueous solution according to the ratio of 1:1, 2:1, 3:1, 4:1, 1:2, 1:3 and 1:4, testing the insecticidal activity of the mixed agent on the tetranychus by adopting a leaf dipping method, recording the number of insect population before and after the medicine, calculating the death rate of the insect population 24h after the medicine, wherein the death rate of the mixed combination is higher than 50% and the highest death rate is the optimal mixed combination, and comparing the co-toxicity factors of the analysis mixture.
Judging the compound synergy degree of the 3 combinations by adopting a co-virulence factor (CTF) method, and taking the synergistic effect when the co-virulence factor ratio is obviously more than 20; antagonism is seen at significantly less than-20, with additive effects between-20 and 20.
Co-virulence factor (CTF) = (actual mortality-expected mortality)/expected mortality x 100.
1.3.3 determination of co-toxicity coefficient of Duocin and hexythiazox mixture at different ratios for Tetranychus cinnabarinus
The toxicity of the spinosad and hexythiazox mixture to Tetranychus cinnabarinus was measured according to method 1.3.1.
The method for calculating the co-toxicity coefficient is as follows
From the result of the bioassay, the method of Finney (1952) probability value analysis is used to respectively calculate the virulence regression equation and LC of spinosad, hexythiazox and the mixture of spinosad and hexythiazox for tetranychus cinnabarinus 50 Value and relative virulence index. Finally, according to the method published in 1960 by Sun Yunpei, the Co-toxicity coefficient (Co-Toxicity Coefficient, abbreviated as C.T.C) of the mixture is calculated according to the following formula. A and B respectively represent two single agents to be mixed, and finally the co-toxicity coefficient of the mixed agent is obtained.
Theoretical (a+b) mixture virulence index=aSingle sheetThe percent of agent toxicity index x A in the cocktail + the percent of agent toxicity index x B in the cocktail.
C.T.C.ltoreq.80 indicates antagonism after mixing two single agents
80< C.T.C <120 means that the two single agents have additive effect after being mixed
And C.T.C.gtoreq.120 represents that the two single agents have synergistic effect after being mixed.
1.4 results and analysis
1.4.1 toxicity of pleocidin and other Agents against Tetranychus cinnabarinus
As can be seen from Table 1, spinosad and hexythiazox were found to have virulence of 67.39ppm and 46.24ppm, respectively, after 72 hours of treatment with the agent.
TABLE 1 toxicity of spinosad and hexythiazox insecticide against Tetranychus cinnabarinus
1.4.2 comparison of acaricidal Activity of Mixed Agents with different ratios
Spinosad and hexythiazox are respectively used for approaching LC 50 The mass concentrations of the values are 70mg/L and 50mg/L, which are compounded in pairs according to different volume ratios, and the results are shown in Table 2. The mortality of test insects treated by the spinosad and the hexythiazox in each proportion is higher than the expected mortality, the co-toxicity factor is between 11.36 and 48, and the additive and synergistic effects are shown, wherein the co-toxicity factor of the spinosad and the hexythiazox in the volume ratio of 3:1 to 4:1 is higher, the two concentrations are respectively 44 and 48, the toxicity of the compound agent to tetranychus cinnabarinus is measured by the two concentrations, and the co-toxicity factor is calculated to further determine whether the two concentrations have the synergistic effects.
Table 2 comparison of indoor biological Activity of combination insecticides with different ratios of spinosad and hexythiazox on Tetranychus cinnabarinus
1.4.3 determination of virulence and Co-toxicity coefficient of different combinations of Mixed medicaments for tetranychus cinnabarinus
The combined action of the 4:1 and 3:1 ratio combination of spinosad and hexythiazox on tetranychus cinnabarinus is measured by adopting a co-toxicity coefficient method. The results show that: the co-toxicity coefficients of the 2 compound combinations are all larger than 120, and the compound combinations have obvious synergistic effect, wherein the co-toxicity coefficients of spinosad and hexythiazox in the ratio of (4:1) reach 531.20, the synergistic effect is most obvious, the insecticidal activity is highest, and the control effect is better.
TABLE 3 virulence and co-toxicity coefficient of different combinations of mixed medicaments on tetranychus cinnabarinus adults
Example 2 field efficacy of a formulated pesticide such as spinosad and hexythiazox on Tetranychus cinnabarinus
The field test is respectively provided with 3 cells for treatment, each cell is divided into a certain limit, and the drug effects of the mixed agents with different concentrations and the single agents with corresponding concentrations are respectively measured. And (3) setting isolation rows among the cells by using clear water as a Contrast (CK). And (3) taking 3 leaves from each point every 24 hours, 48 hours and 72 hours after the medicine, counting the total number of insect population and the number of dead insect population, calculating the death rate of the insect population after different treatment time, and calculating the correction and prevention effects.
As can be seen from table 4: the field control effect of the 4 treatment agents is not obviously different, the control effect of the spinosad and the hexythiazox compound agent on the tetranychus is 52% under the condition that the proportion is 3:1 and the concentration is 30ppm, the control effect of the spinosad and the hexythiazox compound agent on the tetranychus is 60% under the condition that the proportion is 4:1 and the concentration is 30ppm, the control effects of the spinosad and the hexythiazox compound agent are higher than the control effects of the spinosad single agent and the hexythiazox compound agent are obviously different, and the two mixed agents can effectively reduce the field application concentration of the agents and obtain the control effect higher than that of the single agent.
Table 4 results of field trials of formulated insecticides such as spinosad and hexythiazox on tetranychus cinnabarinus
Note that: the different lowercase after the same column of data indicates significant differences (P < 0.05), respectively.
The invention provides a compound pesticide containing spinosad and hexythiazox, as well as an application thought and a method thereof, and the method and the way for realizing the technical scheme are numerous, the above description is only a preferred embodiment of the invention, and it should be pointed out that a plurality of improvements and modifications can be made by those skilled in the art without departing from the principle of the invention, and the improvements and modifications are also regarded as the protection scope of the invention. The components not explicitly described in this embodiment can be implemented by using the prior art.
Claims (5)
1. A composition, characterized in that the active ingredients of the composition comprise spinosad and hexythiazox;
the mass ratio of the spinosad to the hexythiazox is 1:4-4:1.
2. The composition according to claim 1, wherein the mass ratio of spinosad to hexythiazox is 1:1, 2:1, 3:1, 4:1, 1:2, 1:3 or 1:4.
3. An insecticide comprising the composition of claim 1 or 2.
4. Use of a composition according to claim 1 or 2 for the preparation of a pesticide for controlling spider mites.
5. The use according to claim 4, wherein the control of spider mites is control of spider mites in vegetables and/or flowers.
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| CN108330071B (en) * | 2017-01-19 | 2021-10-08 | 中国科学院分子植物科学卓越创新中心 | Beauveria bassiana strain with insect intestinal infection effect and pest control application thereof |
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