CN114507602B - Scenedesmus oleander and culture application thereof - Google Patents
Scenedesmus oleander and culture application thereof Download PDFInfo
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- 241000195663 Scenedesmus Species 0.000 title claims abstract description 42
- 241000893864 Nerium Species 0.000 title description 2
- 238000004321 preservation Methods 0.000 claims abstract description 12
- 239000004519 grease Substances 0.000 claims abstract description 11
- 238000009629 microbiological culture Methods 0.000 claims abstract description 5
- 239000007789 gas Substances 0.000 claims description 18
- 238000005286 illumination Methods 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 13
- 239000001963 growth medium Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 claims description 8
- 239000003546 flue gas Substances 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 238000012136 culture method Methods 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 abstract description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 6
- 230000009919 sequestration Effects 0.000 abstract description 2
- 241000195493 Cryptophyta Species 0.000 description 29
- 239000002028 Biomass Substances 0.000 description 14
- 230000012010 growth Effects 0.000 description 8
- 239000003225 biodiesel Substances 0.000 description 6
- 150000002632 lipids Chemical class 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 108020004463 18S ribosomal RNA Proteins 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000003698 anagen phase Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000002912 waste gas Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
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- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
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- 238000001914 filtration Methods 0.000 description 1
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- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/56—Nitrogen oxides
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/62—Carbon oxides
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- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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- B01D2258/0283—Flue gases
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Abstract
The invention relates to a scenedesmus which is TMJ-D3 and is classified and named scenedesmusScenedesmussp.) The strain is preserved in China general microbiological culture Collection center (CGMCC) in 2 and 5 days of 2018, and the preservation number is CGMCC No.15299. The carbon sequestration efficiency of the oleaginous scenedesmus is high, the grease content is high, and the oleaginous scenedesmus has the function of resisting NOx and high temperature.
Description
Technical Field
The invention belongs to the field of biotechnology and biological energy, and particularly relates to an oleaginous scenedesmus and a culture application thereof.
Background
Since the 21 st century, there is a close relationship between the development of economy and the consumption of energy, the problem of energy safety is a strategic problem in various countries, and the search for a new clean, economical, efficient and renewable energy source becomes a current urgent need to be solved. Biological energy has been an important technological direction of reserve, and as the energy crisis increases, it has become the focus of attention for various countries and algae scientists. Microalgae are important renewable resources among many biomass energy sources. They have the characteristics of wide distribution, large biomass, high photosynthesis efficiency, strong environment adaptability, short growth cycle, high biomass output and the like. The solar energy conversion efficiency of the microalgae can reach 3.5%, and the microalgae is a potential resource for producing medicines, fine chemicals and novel fuels, and fatty acid obtained from the microalgae can be converted into fatty acid methyl ester, namely biodiesel, so that the microalgae are often considered as ideal raw materials for the third-generation biofuel.
The yield of microalgae grease can be improved by breeding excellent algae strains, optimizing culture conditions, improving culture process and designing a high-performance photo-bioreactor, and the high grease yield is a key for realizing industrialization of microalgae biodiesel. At present, the strain with excellent breeding performance is the basis for developing physiological research and industrialized culture of oil-producing microalgae.
CN201110427579.6 discloses a plant of ScenedesmusDesmodesmus sp.) ENN2203A has the characteristics of high temperature resistance and easy collection, has strong environmental adaptability, is suitable for high-density cultivation, can be used for treating sewage containing nitrogen and phosphorus, can be used for reducing emission of carbon dioxide, is used for biodiesel production or is used for aquatic product baits or animal feeds and the like. However, in the cultivation of the strain, the mixed gas of carbon dioxide and air in an amount of 1.5-2% is introduced into the culture solution, and the cultivation is carried out until the 16 th day, and the final dry weight reaches 10.93g/L. In practical application, when CO in the environment 2 When the volume fraction is more than 5v%, most microalgae will be inhibited from growing, affecting the carbon fixation efficiency. And when the introduced fossil fuel exhaust gas contains high concentration of gases such as SOx, NOx, etc., microalgae growth is inhibited and carbon fixation efficiency is lowered.
CN106467897A discloses grease-rich scenedesmus, the strain of which is MH-04, and its classification is named scenedesmus @, and its culture applicationDesmodesmus sp.) The microbial strain has been preserved in China for 24 days of 4 months of 2015The preservation number of the general microbiological center of the preservation management committee is CGMCC No.10764. The scenedesmus can tolerate high concentration of CO 2 And SO 2 CO-containing can be used 2 And SO 2 The waste gas or the flue gas is subjected to illumination autotrophic growth to obtain biomass, the carbon fixing efficiency is high, the obtained biomass is rich in grease, and the production of biodiesel can be performed.
CN107177505A discloses a plant of scenedesmus, HCS-02, named scenedesmus @ in classification, its culture method and applicationScenedesmus sp.) The strain is preserved in China general microbiological culture Collection center (CGMCC) for 24 days, 4 months and 24 days in 2015, and the preservation number is CGMCC No.10766. The scenedesmus can resist lower culture temperature, has large culture temperature range and is easy for open culture. Under different culture conditions, the biomass has a relatively high biomass accumulation speed, and the obtained biomass is rich in starch and grease, and can be used as feed or for biodiesel production after treatment.
The oleaginous microalgae disclosed in the above two patents are directed to tolerance to SO 2 The microalgae bred with specific performances such as low temperature resistance have less ideal effects when being used in other aspects, so that microalgae with more other functions are bred, or microalgae with multiple functions are bred, so that the microalgae are more suitable for industrial application.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an oleaginous scenedesmus and a culture application thereof. The carbon sequestration efficiency of the oleaginous scenedesmus is high, the grease content is high, and the oleaginous scenedesmus has the function of resisting NOx and high temperature.
The classification of the scenedesmus oil production TMJ-D3 is named scenedesmus oil production TMJ-D3Scenedesmus sp.) The strain is preserved in China general microbiological culture Collection center (CGMCC) in 2 and 5 days of 2018, and the preservation number is CGMCC No.15299.
The scenedesmus TMJ-D3 provided by the invention has green algae cells under a microscope, and is often aggregated into groups, wherein the groups are composed of four cells, and the group cells are arranged in a straight line. The individual cells are oval in shape with pigment bodies within, each cell containing a single protein core, the individual cells being about 5-7 μm long and about 2-3 μm wide. The upper and lower ends of the cells at both sides of the population are provided with a long or straight or slightly bent thorns respectively, and both ends of the cells at the middle part and the free parts at the side surfaces of the cells at both sides are free from thorns. The four-cell population is 15-30 microns wide and 10-15 microns long.
The scenedesmus TMJ-D3 provided by the invention can resist CO 2 The concentration of (2) can reach 30% by volume, and the concentration of the tolerant NOx can reach 0.08% by volume.
The scenedesmus TMJ-D3 provided by the invention can resist high temperature, can still grow normally at 40-45 ℃ and keeps higher biomass.
The 18S rDNA gene sequencing analysis result of the scenedesmus TMJ-D3 provided by the invention is shown in a sequence table. According to sequence alignment, the scenedesmus TMJ-D3 of the invention is different from the published 18SrDNA data of scenedesmus strain.
The scenedesmus TMJ-D3 of the invention fixes CO 2 Is used in the field of applications. The strain can tolerate CO in gas 2 The concentration reaches 30v percent, and has better CO 2 Fixing efficiency.
The invention discloses an application of scenedesmus TMJ-D3 in the production of microalgae grease. The algae strain is subjected to illumination autotrophy growth under proper growth conditions to obtain algae cells rich in grease, the dry weight of the algae cells reaches more than 10g/L, and the total grease content of the cells accounts for more than 40% of the dry weight of the cells.
The scenedesmus TMJ-D3 of the invention can purify CO 2 And in NOx exhaust gas or flue gas. CO in exhaust gas or flue gas 2 The concentration is less than or equal to 30v percent, and the concentration of NOx is less than or equal to 0.08v percent.
The invention relates to a culture method of scenedesmus TMJ-D3, which comprises the steps of utilizing BG11 culture medium, SE culture medium and the like to culture in a photobioreactor, and introducing CO 2 And culturing with gas of which the content is 1.0-30v%. Further, the inlet gas contains NOx, and the concentration of the NOx is not higher than 0.08v%. The culture conditions are as follows: the illumination intensity is 1500-20000Lux, the pH value is 6-9, the temperature is 20-45 ℃, the illumination period is 24 hours, and the light-dark time ratio is 14:10. Culturing until the stationary phase is over, and harvesting microalgae cells. The detection shows that the dry weight of the algae cells reaches more than 10g/L, and the total lipid content of the cells accounts for more than 40% of the dry weight of the cells.
Compared with the prior art, the invention has the following beneficial effects:
(1) The scenedesmus oleaginous TMJ-D3 bred by the invention can tolerate high-concentration CO 2 CO can be utilized 2 Long autotrophy and high carbon fixation efficiency are carried out, and the problem of greenhouse effect brought by the current industrial society is relieved.
(2) The strain has the functions of resisting NOx and high temperature. When NOx exists in the waste gas, the inhibition of NOx on photosynthesis of microalgae when the microalgae grows by using the waste gas can be avoided, and the normal growth of the microalgae is maintained. And the temperature which can be tolerated is up to 45 ℃, and the cultivation under high temperature is beneficial to reducing the flue gas cooling cost.
(3) The algae strain has the advantages of high growth rate, short growth period, higher final biomass, more than 10g/L of algae cell dry weight and more than 40% of total cell lipid content, and is suitable for producing biodiesel.
Description of biological Material preservation
The invention provides scenedesmus TMJ-D3Scenedesmus sp.) Preserving in China general microbiological culture Collection center; preservation number: CGMCC No.15299; preservation date: 2.5.2018; preservation address: the institute of microorganisms of national academy of sciences of China, no.1, no. 3, north Chen West Lu, the Korean region of Beijing.
Detailed Description
The technical scheme and effects of the present invention are further described in detail below with reference to specific examples. The embodiments and specific operation procedures are given on the premise of the technical scheme of the invention, but the protection scope of the invention is not limited to the following embodiments.
The experimental methods in the following examples, unless otherwise specified, are all conventional in the art. The experimental materials used in the examples described below, unless otherwise specified, were purchased from conventional biochemical reagent stores. In the present invention, v% is the volume fraction.
EXAMPLE 1 isolation and domestication screening to give scenedesmus TMJ-D3
(1) Obtaining of the starting strain: jiang Quhui water sample 150mL from Jilin province drawing river city drawing of 2016, filtering the water sample with gauze, removingRemoving large impurities, inoculating 50mL of filtered water sample into 200mL of BG11 culture medium for enrichment culture, wherein the illumination intensity of culture is 5000Lux, the temperature is 35 ℃, the light-dark period is 24 hours, the light-dark time ratio is 14:10, and the culture medium is green after about half a month of culture. Diluting the enriched water sample to 10 -5 Culturing in BG11 solid plate under aseptic condition at light intensity of 5000Lux and temperature of 35deg.C for about 10 days, collecting single algae, culturing in shake flask at 35deg.C and light intensity of 5000Lux, culturing for 8 days, observing with microscope to determine whether pure algae is cultivated, if not, repeating the above steps until pure algae is determined. Repeatedly culturing to obtain pure algae strain No. TMJ-3.
（2）CO 2 Is domesticated and cultured: inoculating the pure algae cultured in the shake flask in the step (1) into a microalgae aeration culture apparatus for domestication culture, wherein the illumination intensity is 5000Lux, the temperature is 40 ℃, and CO is in the gas 2 The content of (2) is gradually increased from 5v% to 30v%, each time the content is increased by 5v%, the culture is finished after 8 days, and the domestication culture is repeated for 3 times.
(3) Culturing the domesticated and cultured algae liquid in the step (2) by adopting a plate streaking mode to obtain pure algae seeds, wherein the culturing step is the same as the step (1).
(4) Domestication culture of NOx: introducing CO into the algae liquid in the logarithmic growth phase of the algae strain in the step (3) 2 In the mixed gas with the content of 20v percent, the illumination intensity is 5000Lux, the temperature is 40 ℃, NO gas is injected into the mixed gas to perform tolerance culture on algae seeds, the content of NO in the mixed gas is gradually increased from 0.01v percent to 0.08v percent in the culture process, the content of NO in the mixed gas is increased by 0.01 percent every day, the culture is finished for 8 days, the domestication culture is repeated for 3 times, and the algae liquid with the tolerance to NOx is obtained.
(5) And (3) obtaining pure algae by adopting a plate streaking mode to the algae liquid obtained by domestication in the step (4), and after the cultivation step (1) is finished, selecting larger algae to perform shake flask cultivation to obtain a target algae strain named TMJ-D3.
EXAMPLE 2 identification of algal strains
Extracting DNA of TMJ-D3 strainThe 3 positive clones obtained were sequenced by CTAB and by 18S rDNA gene cloning, to Shanghai Ind. The sequencing analysis result of the 18S rDNA gene is shown in a sequence table. Logging the 18S rDNA sequence into a Genbank database for Blast alignment, and displaying the result and the resultScenedesmus sp.The maximum similarity is that the BLASTN value is 2562, the Max index value is 99.3%, and TMJ-D3 can be determined as scenedesmus @Scenedesmus sp.）。
EXAMPLE 3 application of scenedesmus TMJ-D3
Inoculating TMJ-D3 algae solution in logarithmic growth phase into BG11 culture medium, culturing in photobioreactor, and inoculating culture medium OD 690 0.25. CO is introduced from the bottom of the reactor 2 The content of NO in the flue gas is 30v%, and the content of NO in the flue gas is 0.08v%. In the culture process, the illumination intensity is 8000Lux, the culture temperature is 35 ℃, the pH value is controlled to 7-8, the illumination period is 24 hours, the light-dark time ratio is 14:10, and the culture time is 8 days and is in the stable period. After finishing the culture, centrifugally collecting the algae liquid, vacuum freeze-drying to constant weight at-60 ℃, measuring the dry weight of the algae powder, calculating the biomass yield, and adopting n-hexane: the total lipid content was measured by ethyl acetate method. The detection shows that the biomass yield of the TMJ-D3 strain is 10.5g/L, and the total lipid content of the cells accounts for 42.89% of the dry weight of the cells.
TABLE 1 BG11 Medium
* Table 2 composition of A5+Co solution in Table 1
Example 4 comparison of culture Effect of TMJ-D3 and TMJ-3
Inoculating TMJ-D3 and TMJ-3 algae solution in logarithmic growth phase into BG11 culture medium, culturing in photobioreactor, and OD of the inoculated culture solution 690 0.25. According to the test requirement, CO with different contents is prepared 2 The NO mixture is then introduced from the bottom of the reactor. The illumination intensity is 8000Lux, the temperature is 35 ℃, the pH value is controlled between 7 and 8, the illumination period is 24 hours, the light-dark time ratio is 14:10, the culture time is 8 days, the algae cells are collected after the culture is finished, and the dry weight of the cells is measured. The results are shown in Table 3.
TABLE 3 comparison of culture effects of TMJ-D3 and TMJ-3
As can be seen from Table 3, TMJ-D3 bred by the method has better tolerance to NO than the initial strain TMJ-3. Meanwhile, the removal rate can reach more than 80% by detecting the NO content in the exhaust gas.
Example 5 comparison of culture temperatures of TMJ-D3 and TMJ-3
Inoculating TMJ-D3 and TMJ-3 algae solution in logarithmic growth phase into BG11 culture medium, culturing in photobioreactor, and OD of the inoculated culture solution 690 0.25. CO is introduced from the bottom of the reactor 2 10v% flue gas, wherein the NO content is 0.02. In the culture process, the illumination intensity is 8000Lux, the pH value is controlled to be 7-8, the illumination period is 24 hours, the light-dark time ratio is 14:10, and the culture time is 8 days. After the completion of the culture, algal cells were collected, and the dry weight of the cells was measured, and the results are shown in Table 4.
TABLE 4 comparison of TMJ-D3 and TMJ-3 culture results
As can be seen from Table 4, TMJ-D3 of the present invention had better high temperature tolerance than the initial strain TMJ-3, but neither was tolerant to low temperatures.
Comparative example 1
The difference from example 3 is that the scenedesmus MH-04 disclosed in CN106467897A is adoptedDesmodesmus sp.) The preservation number is CGMCC No.10764, and the culture is finished for 8 days. Through detection, the biomass yield of MH-04 strain is 3.2g/L, and the total lipid content of cells accounts for cells33.5% of dry weight.
Comparative example 2
The difference from example 3 is that Scenedesmus HCS-02 [. Sup.Scenedesmus sp.) The preservation number is CGMCC No.10766, and the culture is finished for 8 days. The detection shows that the biomass yield of the HCS-02 strain is 2.1g/L, and the total lipid content of the cells accounts for 30.56% of the dry weight of the cells.
Sequence listing
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China Petroleum & Chemical Corporation Dalian Petrochemical Research Institute
<120> an oleaginous scenedesmus strain and its culture application
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ttcacgaagc gcatggcctt gtgccggcgc tgttccattc aaatttctgc cctatcaact 300
ttcgatggta ggatgatctg tagccatggt ggtaacgggt gacggaggat tagggttcga 360
ttccggagag ggagcctgag aaacggctac cacatccaag gaaggcagca ggcgcgcaaa 420
ttacccaatc ctgatacggg gaggtagtga caataaataa caataccggg cattttatgt 480
ctggtaattg gaatgagtac aatctaaatc ccttaacgag gatccattgg agggcaagtc 540
tggtgccagc agccgcggta attccagctc caatagcgta tatttaagtt gttgcagtta 600
aaaagctcgt agttggattt cgggtgggtt tcagcggtcc gcctatggtg agcactgctg 660
tggccttcct tactgtcggg gacctgcttc tgggcttcat tgtccgggac agggattcgg 720
catggttact ttgagtaaat tagagtgttc aaagcaggct tacgccgtga atactttagc 780
atggaataac acgataggac tctgccctat tctgttggcc tgtaggagtg gagtaatgat 840
taagaggaac agtcgggggc attcgtattt cattgtcaga ggtgaaattc ttggatttat 900
gaaagacgaa ctactgcgaa agcatttgcc aaggatgttt tcattaatca agaacgaaag 960
ttgggggctc gaagacgatt agataccgtc gtagtctcaa ccataaacga tgccgactag 1020
ggattggcgg acgtttttgc atgactccgt cagcaccttg agagaaatca aagtttttgg 1080
gtttcggggg gagtatggtc gcaaggctga aacttaaagg aattgacgga agggcaccac 1140
caggcgtgga gccgacggct taatttgact caacacggga aaacttacca ggtccagaca 1200
taggaaggat tgacagattg agagctcttt cttgattcta tgggtggtgg tgcatggccg 1260
ttcttagttg gtgggttgtc ttgtcaggtt gattccggta acgaacgaga cctcagcctt 1320
taaatagtca cagtcgcttt ttgcgggtgg tttgacttct tagagggaca gttggcgttt 1380
agtcaacgga agtatgaggc aataacaggt ctagtgatta 1420
Claims (7)
1. The scenedesmus is the scenedesmus which is TMJ-D3, and the classification is named scenedesmus @Scenedesmus sp.) The strain is preserved in China general microbiological culture Collection center (CGMCC) in 2 and 5 days of 2018, and the preservation number is CGMCC No.15299.
2. The method for fixing CO by using scenedesmus as claimed in claim 1 2 Is used in the field of applications.
3. The use of scenedesmus oil production according to claim 1 for the production of microalgae grease.
4. The method for purifying CO-containing scenedesmus according to claim 1 2 And in NOx exhaust gas or flue gas.
5. A method of culturing the scenedesmus oleaginous of claim 1, characterized by: introducing CO into a photobioreactor by utilizing BG11 culture medium or SE culture medium 2 Culturing with gas with the content of 1.0-30 v%.
6. The culture method according to claim 5, wherein: the inlet gas contains NOx, and the concentration of the NOx is not higher than 0.08v percent.
7. The culture method according to claim 5 or 6, wherein: the culture conditions are as follows: the illumination intensity is 1500-20000Lux, the pH value is 6-9, the temperature is 20-45 ℃, the illumination period is 24 hours, and the light-dark time ratio is 14:10.
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Effective date of registration: 20231129 Address after: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec (Dalian) Petrochemical Research Institute Co.,Ltd. Address before: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: DALIAN RESEARCH INSTITUTE OF PETROLEUM AND PETROCHEMICALS, SINOPEC Corp. |