CN102978118A - Scenedesmus sp., CHX1 and use thereof - Google Patents

Scenedesmus sp., CHX1 and use thereof Download PDF

Info

Publication number
CN102978118A
CN102978118A CN2012104691531A CN201210469153A CN102978118A CN 102978118 A CN102978118 A CN 102978118A CN 2012104691531 A CN2012104691531 A CN 2012104691531A CN 201210469153 A CN201210469153 A CN 201210469153A CN 102978118 A CN102978118 A CN 102978118A
Authority
CN
China
Prior art keywords
chx1
scenedesmus
grid algae
algae
grid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2012104691531A
Other languages
Chinese (zh)
Inventor
程海翔
田光明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang University ZJU
Original Assignee
Zhejiang University ZJU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang University ZJU filed Critical Zhejiang University ZJU
Priority to CN2012104691531A priority Critical patent/CN102978118A/en
Publication of CN102978118A publication Critical patent/CN102978118A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention discloses Scenedesmus sp., CHX1 and a use thereof. The Scenedesmus sp., CHX1 is separated from a pig feeding wastewater treatment tank, is named as CHX1, has a class name of Scenedesmus sp., is preserved in the China general microbiological culture collection center and has a preservation number of CGMCC No. 6649. A Scenedesmus sp., CHX1-optimized medium is obtained by preparing solutes of 1ml of an A5+Co solution, 5g of glucose, 3g of NaNO3, 0.04g of K2HPO4.3H2O, 0.075g of MgSO4.7H2O, 0.036g of CaCl2.2H2O, 0.006g of Fe(NH4)3Cl8H10O14, 0.001g of Na2-EDTA, and the balance water into 1L of a solution. The Scenedesmus sp., CHX1 is cultured into a seed culture solution by the Scenedesmus sp., CHX1-optimized medium. The seed culture solution can be used for preparation of biodiesel and treatment on pig feeding wastewater. The Scenedesmus sp., CHX1 can grow well in pig feeding wastewater, can efficiently remove nitrogen and phosphor in wastewater, has a high biological yield and an oil yield, and is suitable for biodiesel production. A fatty acid produced by the Scenedesmus sp., CHX1 has C16-C18 content more than 70%.

Description

A kind of grid algae and application thereof
Technical field
The present invention relates to a kind of grid algae and application thereof, be specifically related to this grid algae in the application of bioenergy and purifying pig-raising field of wastewater.
Technical background
21 century, facing mankind two large crises: energy dilemma and water resources crisis.According to estimates, crude oil reserve and the natural gas reserves in the whole world will be used up after 40 years and 64 years respectively, and therefore, exploiting economy, efficient novel energy are imperative, and wherein biological new forms of energy are one of main study hotspots.Meanwhile, the countries and regions in the whole world 40% are faced with water shortage problem, and the sustainable use of water resources is extremely urgent.Reuse of wastewater provides a new way as the city second water source for the problem in short supply that solves city water resource, but the nitrogen phosphorus of high level causes easily and the eutrophication of water body affects the utilization of sewage in the sewage.Therefore, searching efficiently, the ecological waste water recovery technique is one of important means that solves current water resources crisis cheaply.
Little algae is a class photosynthetic autotrophs unicellular organism, has aboundresources, of a great variety, fast growth, strong adaptability and fat content high.In recent years, along with the mankind to the going deep into of little algae understanding, little algae has been carried out a large amount of correlative studys having obtained increasing concern aspect preparation biofuel and the sewage deep denitrogenation dephosphorizing.Algal grown need to be with nitrogen phosphorus as substrate, therefore utilizes little algae can remove in the sewage pollutents such as nitrogen phosphorus; Simultaneously, little algae can be with CO by photosynthesis 2Be fixed as organic carbon (protein, grease etc.), the triglyceride in the frustule grease is the main raw material of preparation biofuel, therefore, can utilize sewage to cultivate the simultaneously production biofuel of denitrogenation dephosphorizing advanced treatment of microalgae for carrying out sewage.
At present, domestic to carry out the research report that the advanced treatment of wastewater of cultivating based on little algae and production of biodiesel be coupled less, the suitable sewage of screening cultivate and the grease productive rate high, the little algae algae strain that is easy to conversion biodiesel is prerequisite and the key of this technique.And many merely high oil-containing algae kinds take the preparation biofuel as purpose such as chlorella, braunii ball algae might not be in sewage normal growth and a large amount of accumulation greases, and these algae strain ubiquity culture condition require the problems such as high, that biological yield is low or the grease productive rate is low.Therefore, the good algae kind of screening has important practical significance for addressing the above problem.
Summary of the invention
The purpose of this invention is to provide a kind of grid algae and application thereof.
The grid algae is located away from the purification tank for liquid waste of raising pigs, and called after CHX1, its Classification And Nomenclature are Scenedesmus sp., and oneself is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and its deposit number is CGMCC No. 6649.
Described grid algae Optimal Medium is settled to 1 liter with following solute: 1ml A 5+ Co solution, 5g glucose, 3g NaNO 3, 0.04g K 2HPO 43H 2O, 0.075g MgSO 47H 2O, 0.036g CaCl 22H 2O, 0.006g Fe (NH 4) 3C 18H 10O 14, 0.001g Na 2-EDTA, surplus are water; Described A 5+ Co solution is settled to 1 liter with following solute and obtains: 2.86 g H 3BO 3, 1.81 MnCl 2H 2O, 0.222 ZnSO 47H 2O, 0.079 CuSO 45H 2O, 0.39 Na 2MoO 42H 2O, 0.049 Co (NO 3) 26H 2O, surplus are water; The initial pH=8 of Optimal Medium, the starting point concentration of Optimal Medium is OD behind the inoculation grid algae culturing liquid 690=0.16.
Grid algae Scenedesmus sp. is cultivated in the application of described grid algae Optimal Medium, and CHX1 obtains seed culture fluid.
The dry weight biomass of described seed culture fluid and total grease productive rate are respectively: 814.1 mg/litre. day (scope is 809.6 ~ 818.6 mg/litre. day) and 128.3 mg/litre. day (scope is 127.5 ~ 129.0 mg/litre. day); Form that the C16-C18 component content accounts for 70% in the lipid acid of total grease, can be used for preparing biofuel.
The application of described grid algae in waste water is raised pigs in processing.
The described wastewater treatment of raising pigs is for denitrogenating and/or dephosphorization.
Beneficial effect of the present invention: grid algae culture condition requires low, and well-grown in sewage can efficiently be removed the nitrogen phosphorus in the sewage simultaneously.Grid algae self fast growth, biological yield is high, and the grease productive rate is high, can be for the preparation of biofuel.In sum, grid algae CHX1 has broad application prospects in the wastewater treatment coupling high value biomass production application of raising pigs.
Description of drawings
Fig. 1 is the phylogenetic tree that makes up based on 18S rDNA partial sequence.
Fig. 2 is that grid algae CHX1 optimal absorption peak is selected.
Fig. 3 is the growing state of grid algae CHX1 under different training methods.
Fig. 4 is the growing state of grid algae CHX1 under different carbon sources.
Fig. 5 is the growing state of grid algae CHX1 under different glucose dosages.
Fig. 6 is grid algae CHX1 orthogonal optimization bioorganism volume production rate.
Embodiment
Following embodiment is convenient to understand better the present invention, but the present invention is not limited to described embodiment.Experimental technique among the following embodiment if no special instructions, is ordinary method.Used test materials among the following embodiment if no special instructions, is all bought from routine biochemistry reagent shop.Quantitative experiment in following examples all arranges repeated experiments three times, results averaged.
The isolation identification of embodiment 1 grid algae CHX1
One, sample collecting
Contain from the wastewater disposal basin collection of XiaoShan, HangZhou City, Zhejiang Province district plant in July, 2011
The water sample of green alga.
Two, the separation and purification of grid algae CHX1
Purification procedures: with the water sample centrifugal concentrating (5000 r/min, 4 ℃) that gathers, with the sterilized water dilution, it is rear after 0.45 μ m filter membrane to cross 60 microns order protozoon sieves.Repeatedly wash filter membrane with sterilized water, residual cell on the filter membrane is transferred in 12 porocyte culture plates (every Kong Hanyi sterilising treatment BG-11 nutrient solution 1ml, sterilization method is 121 ℃ of lower high pressure steam sterilizations 20 minutes), place and carry out enrichment culture in the illumination box (culture condition is whole day illumination, and intensity of illumination is 40 μ mol photon/ m 2. s, temperature is 25 ℃).After the enrichment culture 7 days, suitably dilute algae liquid, draw dilution algae liquid and be applied on the BG-11 solid medium that contains 1.5% agar, (culture condition: whole day illumination, intensity of illumination are 40 μ mol photon/ m to place the interior cultivation a few days of illumination box 2. s, temperature is 25 ℃), then select single bacterium colony and in liquid B G-11 nutrient solution, carry out again enlarged culturing (culture condition: whole day illumination, intensity of illumination are 40 μ mol photon/ m 2. s, temperature is 25 ℃, shaking table speed 150 r min -1).Obtain the pure algae strain of a strain, called after CHX1.
Three, the evaluation of grid algae CHX1
1. the identification of morphology of grid algae CHX1
Cell is green, the normal gathering in groups; Rounded or the elliposoidal of individual cells form, diameter are 3-6 μ m; Chromatoplast Zhousheng includes fat and drips; Cell contains an apparent pyrenoids.
2. the Molecular Identification of grid algae CHX1
Collect the cell of exponential phase of growth, extract test kit (Bioisystech Co., Ltd is built up in Nanjing) with plant genome DNA and extract genomic dna, take it as template amplification 18S rDNA gene fragment.The pcr amplification system is 20 μ l, wherein contains 1 μ l 50ng dna profiling, 0.2 μ l 1.6-Unit Taq polysaccharase, 2 μ l 10xPCR reaction systems, 2 μ l 2.5mM dNTP, positive each 0.5 μ l of anti-primer (0.2 μ M), two steaming aqua sterilisa 13.8 μ l.The pcr amplification program is: 94 ℃ of denaturation 5min; 94 ℃ of sex change 40s, 58 ℃ of renaturation 35s, 72 ℃ are extended 35s, totally 36 circulations; 72 ℃ are extended 10min again.
The primer that is used for amplification 18S rDNA is eukaryote 18S rDNA specific primer, and sequence is as follows:
Forward primer (18SF): 5 '-CAGGTCTGTGATGCCC-3 ';
Reverse primer (18SR): 5 '-ACGGGCGGTG TGTAC-3 ';
Pcr amplification product reclaims test kit with glue and reclaims after 1% agarose gel electrophoresis separates, transfer to the order-checking of the large gene sequencing company limited of Shanghai China.Pcr amplification product is 18S rDNA partial sequence, and sequencing result is shown in the sequence of sequence table.
Sequencing result is through NCBI website BLAST compare of analysis, CHX1 and Scenedesmaceaesp .(AY197639) it is 96% that Query coverage value reaches 100%, Max-Ident.Carry out the multisequencing homology analysis with MEGA 4.0 softwares, and according to NJ method phylogenetic tree construction (see Fig. 1, the numerical value on the branch is bootstrapping 100).Little algae CHX1 and Scenedesmaceaesp .Gather well at a branch.
Result based on identification of morphology and Molecular Identification, CHX1 is accredited as grid algae (Scenedesmus sp.), oneself is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on October 10th, 2012, and its deposit number is CGMCC No. 6649.
Embodiment 2 grid algae CHX1 growth conditionss are optimized
One, the best extinction density value of grid algae CHX1 determines
For better describing grid algae CHX1 growth, determine the best extinction density value of grid algae CHX1, the BG11 nutrient solution that will contain little algae carries out automatically scanning and determines in the 600-700nm scope.Scanning result is seen Fig. 2.
Two, the optimization of grid algae CHX1 growth conditions is determined
In order to make the faster and better growth of grid algae CHX1, carry out training method, carbon source, glucose dosage and culture condition Combinatorial Optimization at the BG-11 medium base.
1. training method optimization is determined
Take the BG-11 substratum as the basis, establish light autotrophy, heterotrophism and raise together with three kinds of training methods, wherein light autotrophy culture condition is illumination+BG-11 nutrient solution; The heterotrophism culture condition is BG-11 nutrient solution+1g/L glucose; Raising together with culture condition is illumination+BG-11 nutrient solution+1g/L glucose.Grid algae CHX1 is seeded to respectively in each nutrient solution (with OD 690To be 0.637 grid algae CHX1 be inoculated in each nutrient solution the OD of grid algae in nutrient solution with 10% volume ratio to value 690Value is about 0.067), to cultivate 3 days for 25 ℃, intensity of illumination is 40 μ mol photon/ m 2. s.Detect the OD in each nutrient solution 690Value is according to OD 690And the conversion relation formula between the frustule dry matter is calculated the biomass productive rate, the results are shown in Figure 3.
2. carbon source optimizing is determined
In the BG-11 substratum, remove the basis of yellow soda ash composition and add different carbon sources, with etc. the volumetric molar concentration carbon content set each carbon source addition.Grid algae CHX1 is seeded to respectively in each nutrient solution (with OD 690To be 1.70 grid algae CHX1 be inoculated in each nutrient solution the OD of grid algae in nutrient solution with 20% volume ratio to value 690Value is about 0.38), to cultivate 3 days for 25 ℃, intensity of illumination is 40 μ mol photon/ m 2. s.Detect the OD in each nutrient solution 690Value detects the OD in each nutrient solution 690Value is according to OD 690And the conversion relation formula between the frustule dry matter is calculated the biomass productive rate, the results are shown in Figure 4.
3. the optimization of glucose dosage is determined
On the basis of determining optimum carbon source, in cultivating, BG-11 substitutes yellow soda ash as carbon source with glucose, set different glucose dosages.Grid algae CHX1 is seeded to respectively in each nutrient solution (with OD 690To be 0.8 grid algae CHX1 be inoculated in each nutrient solution the OD of grid algae in nutrient solution with 10% volume ratio to value 690Value is about 0.08), to cultivate 3 days for 25 ℃, intensity of illumination is 40 μ mol photon/ m 2. s.Detect the OD in each nutrient solution 690Value is according to OD 690And the conversion relation formula between the frustule dry matter is calculated the biomass productive rate, the results are shown in Figure 5.
4. the optimal culture condition combination is determined
Adopt L 9(3 4) the further optimum culture condition combination of orthogonal design table, take glucose dosage, nitrate dosage, initial pH and inoculum density as investigating index, the screening top condition makes up, and the specific design scheme sees Table 1.Grid algae CHX1 is seeded to respectively in each nutrient solution, cultivated 3 days for 25 ℃, intensity of illumination is 40 μ mol photon/ m 2.s.Detect the OD in each nutrient solution 690Value is according to OD 690And the conversion relation formula between the frustule dry matter is calculated the biomass productive rate, and concrete outcome is seen Fig. 6.
Table 1 L 9(3 4) the orthogonal design table design
Figure 522106DEST_PATH_IMAGE002
According to above-mentioned optimum result, determined optimum culture condition and the Optimal Medium of grid algae CHX1.Optimal Medium is settled to 1 liter with following solute water and obtains: add 1ml A 5+ Co solution, 5g glucose, 3g NaNO 3, 0.04g K 2HPO 43H 2O, 0.075g MgSO 47H 2O, 0.036g CaCl 22H 2O, 0.006g Fe (NH 4) 3C 18H 10O 14, 0.001g Na 2-EDTA.
A 5+ Co solution is following solute water to be settled to 1 liter obtain: 2.86 g H 3BO 3, 1.81 MnCl 2H 2O, 0.222 ZnSO 47H 2O, 0.079 CuSO 45H 2O, 0.39 Na 2MoO 42H 2O, 0.049 Co (NO 3) 26H 2O.The initial pH=8 of substratum, inoculum size is 10%(v/v, OD 690=0.16).
Growth and the grease yield of embodiment 3 grid algae CHX1
One, the cultivation of grid algae CHX1
According to condition and the culture medium culturing grid algae CHX1 that embodiment 2 optimizes, 25 ℃ of culture temperature, whole day illumination 40 μ mol photon/ m 2.s cultivated 3 days under the condition.
Two, the growth of grid algae CHX1 and grease yield are determined
1. grid algae CHX1 biomass is determined
Get nutrient solution and place centrifuge tube, centrifugal 10min(10000 r/min, 4 ℃), abandoning supernatant is transferred to the concentrated biological amount on the tinfoil paper lyophilize.Every liter of nutrient solution obtain the dry frond of 2.44g (the biomass average yield is 814.1 mg/L. d, and scope is 809.6 ~ 818.6 mg/litre. day), the biomass productive rate is higher.
2. grid algae CHX1 grease productive rate is determined
(weight is to take by weighing certain mass lyophilize algae sample W 0 ), add solvent (chloroform: methyl alcohol=2:1, volume ratio), 30 ℃ of ultrasound bath 30min, leave standstill for a moment, collect supernatant liquor and cross glass fibre filter, filtrate transferring to contains in the centrifuge tube of 0.9% NaCl solution, shake up the centrifugal 10min of static rear centrifugal 1000 r/min, read lower floor's liquid volume and (be designated as V 0 ), draw certain volume and (be designated as V 1 ) lower floor's liquid be transferred to dry and (being designated as of weighing W 1 ) in the new centrifuge tube, nitrogen blows to the organic solvent volatilization to be done, the weighing centrifuge tube is heavy (to be designated as W 2 ), calculating grid algae fat contents (%)=( W 2 - W 1 ) * V 0 / ( W 0 * V 1 ) * 100, grease productive rate (mg/L. d)=fat content (%) * biomass productive rate (mg/L. d).The result shows that average grease productive rate is 128.3 mg/L. d, and scope is 127.5 ~ 129.0 mg/litre. day (fat content is 15.8%).
3. grid algae CHX1 Determination of Fatty acid Components
Take by weighing 0.1g lyophilize algae powder and place hydrolysis pipe with nut, add 8ml 0.5M KOH-CH 3OH solution fills N 2Seal behind the 1min, with SCQ-250 type ultrasonic cleaner (33kHz, 250W) ultrasonic extraction 5min.Abundant compound sample is placed 100 ℃ of water-bath 15min, be cooled to room temperature, use 0.7M HCl-CH 3OH solution is regulated pH＜2, again 100 ℃ of water-bath 15min.After being cooled to room temperature, add the saturated NaCl solution of 2ml, then extract at twice with the 2ml normal hexane.United extraction liquid blows to 20 ~ 50 μ l with nitrogen and to analyze for GC-MS in tool plug centrifuge tube.
The GC-MS instrument includes DB-5-MS capillary chromatographic column and ion flame detector, and routine analyzer is 80 ℃ of column temperatures, and then constant temperature 5min is warming up to 290 ℃ with 4 ℃/min speed, constant temperature 5min.The sampler temperature is 250 ℃, and detector temperature is 230 ℃.Carrier gas is helium, and carrier gas flux is 1ml/min.The result shows that take short chain fatty acid as main, wherein C16-C18 accounts for 70% of total fatty acid content, is suitable for the production biofuel in the grid algae CHX1 fatty acid component.Specifically see Table 2.
Table 2 grid algae CHX1 Analysis of Fatty Acids Composition
Fatty acid type Account for the content (%) of total fatty acids
C12:0 0.13
C14:0 0.44
C16:0 28.3
C16:2 1.61
C18:0 0.86
C18:1 28.1
C18:2 8.91
C18:3 2.18
C20:1 0.62
C22:0 0.13
Unknown 28.72
Situation is removed in raise pigs in waste water growth and nitrogen phosphorus of embodiment 4, grid algae CHX1
The waste water of raising pigs is taken from the water outlet of inhabitants in Xiaoshan pig farm stabilization pond, adopt air stripping method (aeration rate 1500 ml/min, aeration time 12 h/d, continuous aeration 7d) that breeding wastewater is carried out pre-treatment in the laboratory, reduce ammonia-nitrogen content, alleviate the growth inhibitory effect to the grid algae.Breeding wastewater after the pre-treatment is crossed 0.45 μ m filter membrane, and unsterilised, water-quality guideline sees Table 3.
The table 3 waste water quality index of raising pigs
One, the cultivation of grid algae CHX1
The waste water of raising pigs is used for need carrying out pre-treatment before little algae is cultivated, to remove high-concentration ammonia-nitrogen to the growth inhibitory effect of little algae.BG-11 nutrient solution (the OD that will contain grid algae CHX1 690=1.24) with 20%(v/v) be seeded to (the bottled 250ml of each taper) in the 500ml Erlenmeyer flask, whole day illumination (intensity of illumination 100 μ mol photo/m 2.s) blast simultaneously and contain 5% CO 2Air (aeration rate is 100ml/min, continuous aeration), 27 ℃ of lower incubation times 8 days.
Two, removal efficiency of nitrogen and phosphorus determines
Get the waste water nutrient solution and carry out determination of total nitrogen content (GB11894-89, alkaline alkaline potassium per-sulfate digestion ultraviolet spectrophotometry is adopted in the water quality total nitrogen concentration determination), ammonia nitrogen determination (HJ535-2009, the nessler reagent spectrophotometry is adopted in the Water quality ammonia nitrogen concentration determination) and total phosphorus determination (GB/T 11893-1989, ammonium molybdate scene light-intensity method is adopted in the water quality total phosphorus concentration determination), the results are shown in Table 4 (mean values of three repetitions).
Water phosphorus content before and after table 4 grid algae CHX1 cultivates
Total nitrogen content (mg/L) before cultivating Total nitrogen content (mg/L) after cultivating Nitrogen removal rate (%)
110.3 13.4 87.9
Ammonia-nitrogen content (mg/L) before cultivating Ammonia-nitrogen content (mg/L) after cultivating Ammonia nitrogen removal frank (%)
109.3 3.0 97.3
Total phosphorous (mg/L) before cultivating Total phosphorous (mg/L) after cultivating Total tp removal rate (%)
8.53 0.58 93.2
Three, biomass and fat content are measured
Measuring method is with the step of embodiment 2.The average biomass productive rate is that 869 mg/L. d(scopes are 867.4 ~ 870.6 mg/L. d), fat content is 13.6%, average grease productive rate is that 118.2 mg/L. d(scopes are 118.0 ~ 118.4 mg/L. d).
The result shows, grid algae CHX1 lives in after the pre-treatment in the breeding wastewater, and biomass and grease productive rate are all higher, and nitrogen, phosphorus are had stronger removal effect, are a strain has very large potentiality in the wastewater treatment coupling high value biomass production application of raising pigs little algaes.

Claims (6)

1. grid algae, it is characterized in that: be located away from the purification tank for liquid waste of raising pigs, called after CHX1, its Classification And Nomenclature are Scenedesmus sp., oneself is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and its deposit number is CGMCC No. 6649.
2. a grid algae Optimal Medium as claimed in claim 1 is characterized in that: following solute is settled to 1 liter: 1ml A 5+ Co solution, 5g glucose, 3g NaNO 3, 0.04g K 2HPO 43H 2O, 0.075g MgSO 47H 2O, 0.036g CaCl 22H 2O, 0.006g Fe (NH 4) 3C 18H 10O 14, 0.001g Na 2-EDTA, surplus are water; Described A 5+ Co solution is settled to 1 liter with following solute and obtains: 2.86 g H 3BO 3, 1.81 MnCl 2H 2O, 0.222 ZnSO 47H 2O, 0.079 CuSO 45H 2O, 0.39 Na 2MoO 42H 2O, 0.049 Co (NO 3) 26H 2O, surplus are water; The initial pH=8 of Optimal Medium, the starting point concentration of Optimal Medium is OD behind the inoculation grid algae culturing liquid 690=0.16.
3. the application of a grid algae Optimal Medium as claimed in claim 2 is characterized in that: described grid algae Optimal Medium is cultivated grid algae Scenedesmus sp., and CHX1 obtains seed culture fluid.
4. application as claimed in claim 3 is characterized in that: the dry weight biomass of described seed culture fluid and total grease productive rate are respectively: the 809.6-818.6 mg/litre. sky and 127.5-129.0 mg/litre. day; Form that the C16-C18 component content accounts for 70% in the lipid acid of total grease, for the preparation of biofuel.
5. the application of a grid algae as claimed in claim 1 in waste water is raised pigs in processing.
6. application as claimed in claim 5 is characterized in that: the described wastewater treatment of raising pigs is for denitrogenating and/or dephosphorization.
CN2012104691531A 2012-11-20 2012-11-20 Scenedesmus sp., CHX1 and use thereof Pending CN102978118A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2012104691531A CN102978118A (en) 2012-11-20 2012-11-20 Scenedesmus sp., CHX1 and use thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2012104691531A CN102978118A (en) 2012-11-20 2012-11-20 Scenedesmus sp., CHX1 and use thereof

Publications (1)

Publication Number Publication Date
CN102978118A true CN102978118A (en) 2013-03-20

Family

ID=47852460

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2012104691531A Pending CN102978118A (en) 2012-11-20 2012-11-20 Scenedesmus sp., CHX1 and use thereof

Country Status (1)

Country Link
CN (1) CN102978118A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105132404A (en) * 2015-10-12 2015-12-09 山东大学 Method for fast accumulating grease through ultrasonic stimulation micro algae
CN110982859A (en) * 2019-12-23 2020-04-10 哈尔滨工业大学 Method for producing carbohydrate by using pig-raising sewage
CN114507602A (en) * 2020-10-28 2022-05-17 中国石油化工股份有限公司 Scenedesmus for producing oil and culture application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102250773A (en) * 2011-05-31 2011-11-23 中国科学院青岛生物能源与过程研究所 Scenedesmus as well as culturing method and application thereof
CN102442726A (en) * 2011-10-26 2012-05-09 南昌大学 Fungi-mediated microalgae immobilization wastewater treatment method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102250773A (en) * 2011-05-31 2011-11-23 中国科学院青岛生物能源与过程研究所 Scenedesmus as well as culturing method and application thereof
CN102442726A (en) * 2011-10-26 2012-05-09 南昌大学 Fungi-mediated microalgae immobilization wastewater treatment method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHENG H ET AL: "GenBank JX258841.1", 《GENBANK JX258841.1》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105132404A (en) * 2015-10-12 2015-12-09 山东大学 Method for fast accumulating grease through ultrasonic stimulation micro algae
CN110982859A (en) * 2019-12-23 2020-04-10 哈尔滨工业大学 Method for producing carbohydrate by using pig-raising sewage
CN110982859B (en) * 2019-12-23 2023-05-05 哈尔滨工业大学 Method for producing carbohydrate by utilizing pig raising sewage
CN114507602A (en) * 2020-10-28 2022-05-17 中国石油化工股份有限公司 Scenedesmus for producing oil and culture application thereof
CN114507602B (en) * 2020-10-28 2023-07-04 中国石油化工股份有限公司 Scenedesmus oleander and culture application thereof

Similar Documents

Publication Publication Date Title
Wu et al. Lipid accumulating microalgae cultivation in textile wastewater: Environmental parameters optimization
CN102093955B (en) Chlorella strain and application thereof
CN104611228B (en) Highly oil-containing monoraphidium and culture and application thereof
CN102703326B (en) Plant height CO2Tolerance and fixed rate microalgae and breeding method thereof
CN101285075B (en) Coupling method for biogas fermentation and autotrophic freshwater microalgae culture
CN106467896B (en) A kind of kelvin being resistant to high PH intends chlorella and its culture application
Zhang et al. Domestic wastewater treatment and biofuel production by using microalga Scenedesmus sp. ZTY1
Osundeko et al. Implications of sludge liquor addition for wastewater-based open pond cultivation of microalgae for biofuel generation and pollutant remediation
CN104388315A (en) Scnedesmus quadricauda for efficiently treating typical domestic sewage, and culture method and application thereof
CN102815839A (en) Method and device for low-carbon emission energy regeneration by coupling waste gas and waste water
CN102583767B (en) System for treating sewage and producing biological oil by using microalgae and method
CN104004658B (en) One strain utilizes high concentration CO2show the limnetic chlorella of heterotrophic growth characteristic
CN103184156B (en) Scenedesmus algal strain and its use
Solmaz et al. Effect of sludge retention time on biomass production and nutrient removal at an algal membrane photobioreactor
CN103881923A (en) Method for culturing microalgae by using coking wastewater
CN103555584B (en) Acquisition and application of grease-producing monoraphidium LB50
CN104611227A (en) Scenedesmus obliquus with tolerance to high pH and breeding method thereof
CN106467897A (en) A kind of rich grease-contained scenedesmus and its culture application
CN103571754A (en) Chlorella Chlorella sp.HQ culture method and application in water purification and oil production
CN102746991B (en) Method for carrying out heterotrophic culture on chlorella with acid-producing wastewater
Xu et al. Screening of freshwater oleaginous microalgae from South China and its cultivation characteristics in energy grass digestate
CN102978118A (en) Scenedesmus sp., CHX1 and use thereof
CN103540533B (en) Obtaining and application of oil-producing monoraphidium LB59
CN103160440B (en) The one algae strain of strain grid algae and application thereof
CN102943044B (en) Scenedesmus sp. and use thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20130320