CN114507602A - Scenedesmus for producing oil and culture application thereof - Google Patents
Scenedesmus for producing oil and culture application thereof Download PDFInfo
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- 241000195663 Scenedesmus Species 0.000 title claims abstract description 56
- 238000004321 preservation Methods 0.000 claims abstract description 10
- 238000009629 microbiological culture Methods 0.000 claims abstract description 6
- 210000004027 cell Anatomy 0.000 claims description 37
- 239000002028 Biomass Substances 0.000 claims description 14
- 239000007789 gas Substances 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 13
- 238000005286 illumination Methods 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 9
- 239000003546 flue gas Substances 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 235000007122 Scenedesmus obliquus Nutrition 0.000 claims description 4
- 238000012136 culture method Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 210000000349 chromosome Anatomy 0.000 claims description 2
- 210000004940 nucleus Anatomy 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 241000195662 Tetradesmus obliquus Species 0.000 claims 2
- 241000795633 Olea <sea slug> Species 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 6
- 230000009919 sequestration Effects 0.000 abstract description 3
- 241000195493 Cryptophyta Species 0.000 description 25
- 229910002092 carbon dioxide Inorganic materials 0.000 description 10
- 230000012010 growth Effects 0.000 description 10
- 150000002632 lipids Chemical class 0.000 description 7
- 239000003225 biodiesel Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000004519 grease Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 108020004463 18S ribosomal RNA Proteins 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000002912 waste gas Substances 0.000 description 4
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 3
- 241000305506 Desmodesmus Species 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 244000249201 Scenedesmus obliquus Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000001651 autotrophic effect Effects 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 240000002834 Paulownia tomentosa Species 0.000 description 1
- 235000010678 Paulownia tomentosa Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
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- 239000002551 biofuel Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
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- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 235000019387 fatty acid methyl ester Nutrition 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 239000002803 fossil fuel Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
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- 239000012535 impurity Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/56—Nitrogen oxides
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/62—Carbon oxides
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- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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Abstract
The invention relates to Scenedesmus elegans for producing oil and culture application thereof, wherein the Scenedesmus elegans strain is Scenedesmus elegans TMJ-D3 which is classified and named as Scenedesmussp.) The strain has been preserved in China general microbiological culture Collection center (CGMCC) at 2018, 2 and 5, with the preservation number of CGMCC No. 15299. The oil-producing scenedesmus provided by the invention has high carbon sequestration efficiency and high oil content, and particularly has the functions of resisting NOx and high temperature.
Description
Technical Field
The invention belongs to the fields of biotechnology and biological energy, and particularly relates to oil-producing scenedesmus and culture application thereof.
Background
Since the 21 st century, the development of economy has a close relationship with the consumption of energy, the problem of energy safety is a strategic problem of each country, and the problem of searching for a clean, economic, efficient and renewable new energy is a problem which is urgently needed to be solved at present. Biological energy has been used as an important reserve technology direction, and with the increasing energy crisis, it is becoming the focus of attention of various countries and algorists. Among the many biomass energy sources, microalgae are important renewable resources. They have the characteristics of wide distribution, large biomass, high photosynthesis efficiency, strong environment adaptability, short growth period, high biomass yield and the like. The solar energy conversion efficiency of the microalgae can reach 3.5 percent, the microalgae is a potential resource for producing medicines, fine chemicals and novel fuels, and fatty acid obtained from the microalgae can be converted into fatty acid methyl ester, namely biodiesel, so the microalgae is often considered as an ideal raw material of third-generation biofuel.
The yield of the microalgae grease can be improved by breeding excellent microalgae strains, optimizing culture conditions, improving culture process and designing a high-performance photobioreactor, and the high grease yield is the key for realizing the industrialization of the microalgae biodiesel. At present, the breeding of the algae strains with superior performance is the basis for developing the physiological research and the industrialized culture of the oil-producing microalgae.
CN201110427579.6 discloses a Scenedesmus strain (Scenedesmus sp.Koch)Desmodesmus sp.) ENN2203A, the strain has the characteristics of high temperature resistance and easy collection, has strong environmental adaptability, is suitable for high-density culture, can be used for treating nitrogen-phosphorus-containing sewage, can be used for reducing carbon dioxide emission, and can be used for producing biodiesel or used as bait for aquatic products or animal feed and the like. However, in the course of culturing the algal strain, by introducing a mixed gas of carbon dioxide and air in an amount of 1.5 to 2% into the culture medium,the culture was carried out until day 16, and the final dry weight reached 10.93 g/L. In practical application, when CO is in the environment2When the volume fraction is more than 5v%, the growth of most microalgae is inhibited, and the carbon fixation efficiency is influenced. And when the introduced fossil fuel waste gas contains gases such as SOx, NOx and the like with high concentration, the growth of microalgae can be inhibited and the carbon fixation efficiency can be reduced.
CN106467897A discloses a Scenedesmus rich in oil and its culture application, the algae strain is MH-04, its classification name is Scenedesmus ((A) Scenedesmus)Desmodesmus sp.) And has been preserved in China general microbiological culture Collection center (CGMCC) at 24 days 4 months 4 in 2015 with the preservation number of CGMCC No. 10764. The Scenedesmus can tolerate high concentration of CO2And SO2Use of CO-containing2And SO2The waste gas or the smoke is irradiated for autotrophic growth to obtain the biomass, the carbon fixation efficiency is high, the obtained biomass is rich in grease, and the production of the biodiesel can be carried out.
CN107177505A discloses Scenedesmus as well as culture method and application thereof, the Scenedesmus is HCS-02 which is classified and named Scenedesmus (Scenedesmus)Scenedesmus sp.) And has been preserved in China general microbiological culture Collection center (CGMCC) at 24 days 4 months 4 in 2015 with the preservation number of CGMCC No. 10766. The scenedesmus can tolerate a lower culture temperature, has a large culture temperature range, and is easy for open culture. The biomass accumulation speed is high under different culture conditions, and the obtained biomass is rich in starch and grease and can be used as feed or for producing biodiesel after being treated.
The oleaginous microalgae disclosed in the two patents are directed to SO tolerance2The effect of breeding the microalgae with specific performances such as low temperature resistance and the like is not ideal when the microalgae is used in other aspects, so that the microalgae with more other functions or the microalgae with multiple functions need to be bred, so that the microalgae is more suitable for industrial application.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides oil-producing scenedesmus and culture application thereof. The oil-producing scenedesmus provided by the invention has high carbon sequestration efficiency and high oil content, and particularly has the functions of resisting NOx and high temperature.
The oil-producing scenedesmus TMJ-D3 is named as scenedesmus (Scenedesmus sp.) (Scenedesmus sp.) And the strain is preserved in China general microbiological culture Collection center (CGMCC) at 2018, 2 and 5, with the preservation number of CGMCC 15299.
The scenedesmus TMJ-D3 provided by the invention has green algae cells under a microscope, and is often aggregated into a group, wherein the group consists of four cells, and the cells of the group are arranged in a straight line. The single cells are in the shape of long ova and contain chromosomes, each cell contains a protein nucleus, and the single cells are about 5-7 μm long and about 2-3 μm wide. The upper and lower ends of the cells on both sides of the colony have a long or straight or slightly curved prick, and both ends of the cells in the middle part and the side free parts of the cells on both sides have no prick. The width of the four-cell population is 15-30 microns, and the puncture length is 10-15 microns.
The Scenedesmus TMJ-D3 provided by the invention can tolerate CO2The concentration of (2) can reach 30v%, and the concentration of the tolerant NOx can reach 0.08 v%.
The Scenedesmus TMJ-D3 provided by the invention can endure high temperature, can still normally grow at 40-45 ℃, and keeps higher biomass.
The 18S rDNA gene sequencing analysis result of Scenedesmus TMJ-D3 provided by the invention is shown in a sequence table. According to the sequence alignment, the Scenedesmus TMJ-D3 of the invention has difference with the published 18SrDNA data of Scenedesmus strain.
Scenedesmus TMJ-D3 of the invention is used for fixing CO2The use of (1). The strain can tolerate CO in gas2The concentration reaches 30v%, and the better CO is obtained2The efficiency is fixed.
The Scenedesmus TMJ-D3 disclosed by the invention is applied to production of microalgae oil. The algal strain is subjected to light autotrophic growth under a proper growth condition to obtain algal cells rich in grease, the dry weight of the algal cells reaches more than 10g/L, and the total lipid content of the cells accounts for more than 40% of the dry weight of the cells.
Scenedesmus TMJ-D3 of the invention is used for purifying CO2And in NOx exhaust or flue gases. CO in exhaust gas or flue gas2The concentration is less than or equal to 30v percent, and the concentration of NOx is less than or equal to 0.08v percent.
Culture of Scenedesmus Gmelini TMJ-D3 of the present inventionThe method comprises culturing in photobioreactor with BG11 culture medium, SE culture medium, etc., introducing CO2Gas culture with a content of 1.0-30 v%. Further, the gas to be introduced contains NOx at a concentration of not higher than 0.08 v%. The culture conditions were: the illumination intensity is 1500-. And (5) harvesting the microalgae cells after the culture is finished in the stationary phase. Through detection, the dry weight of the algae cells reaches more than 10g/L, and the total lipid content of the cells accounts for more than 40 percent of the dry weight of the cells.
Compared with the prior art, the invention has the following beneficial effects:
(1) the oil-producing scenedesmus TMJ-D3 bred by the invention can tolerate high-concentration CO2Can utilize CO2The carbon sequestration is high, and the problem of greenhouse effect brought by the current industrial society is solved.
(2) The strain has the functions of resisting NOx and high temperature. When NOx exists in the waste gas, the inhibition of the NOx on the photosynthesis of the microalgae during the growth of the microalgae by using the waste gas can be avoided, and the normal growth of the microalgae can be maintained. And the temperature which can be endured is up to 45 ℃, and the flue gas cooling cost is reduced by culturing at high temperature.
(3) The algal strain has the advantages of high growth rate, short growth period, high final biomass, dry weight of algal cells of more than 10g/L and total cell lipid content of more than 40 percent of the dry weight of the cells, and is suitable for producing biodiesel.
Biological material preservation instructions
Scenedesmus TMJ-D3 (provided by the invention)Scenedesmus sp.) The culture is preserved in the China general microbiological culture Collection center; the preservation number is: CGMCC No. 15299; the preservation date is as follows: year 2018, month 2 and day 5; and (4) storage address: the institute of microbiology, national academy of sciences No. 3, Xilu No.1, Beijing, Chaoyang, Beijing.
Detailed Description
The technical solution and effects of the present invention will be further described in detail with reference to the following specific examples. The embodiments are implemented on the premise of the technical scheme of the invention, and detailed implementation modes and specific operation processes are given, but the protection scope of the invention is not limited to the following embodiments.
The experimental procedures in the following examples are, unless otherwise specified, conventional in the art. The experimental materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified. In the present invention, v% is a volume fraction.
Example 1 isolation and acclimatization screening to obtain Scenedesmus TMJ-D3
(1) Obtaining starting algae strains: taking 150mL of water sample from Jiang of Jiang city of Tung of Jilin province in 10 months in 2016, filtering the water sample by using gauze to remove large impurities, taking 50mL of the filtered water sample, inoculating the filtered water sample into 200mL of BG11 culture medium for enrichment culture, wherein the illumination intensity of the culture is 5000Lux, the temperature is 35 ℃, the light-dark period is 24 hours, the light-dark time ratio is 14:10, and the culture medium is green after about half a month of culture. Diluting the water sample of enrichment culture to 10-5And coating the mixture on a BG11 solid plate under aseptic conditions for culture, wherein the illumination intensity of the culture is 5000Lux, the temperature is 35 ℃, green single algae colonies appear on the plate after about 10 days of culture, selecting the single algae colonies to culture on a shake flask, the culture temperature is 35 ℃, the illumination intensity is 5000Lux, after 8 days of culture, observing by a microscope to determine whether the single algae colonies are pure algae strains, and repeating the steps until the pure culture algae strains are determined. After repeated cultivation, the pure strain with the number of TMJ-3 is obtained.
（2）CO2The domestication culture: inoculating the pure algae cultured in the shake flask in the step (1) into a microalgae aeration culture device for acclimatization culture, wherein the illumination intensity is 5000Lux, the temperature is 40 ℃, and CO in the introduced gas2The content of (A) was gradually increased from 5v% to 30v%, each time increased by 5v%, and the culture was terminated after 8 days, and the acclimation culture was repeated 3 times.
(3) And (3) culturing the algae liquid domesticated and cultured in the step (2) by adopting a plate streaking mode to obtain pure algae seeds, wherein the culturing step is the same as the step (1).
(4) Acclimatization and culture of NOx: introducing CO into the algae liquid in the logarithmic growth phase of the algae strains in the step (3)2Adding NO gas into 20v% mixed gas at 40 deg.C and illumination intensity of 5000Lux, performing tolerant culture of algae, and culturingIn the process, the content of NO in the mixed gas is gradually increased from 0.01v% to 0.08v%, the content is increased by 0.01v% every day, the culture is finished for 8 days, and the acclimation culture is repeated for 3 times to obtain the NOx-resistant algae solution.
(5) And (3) obtaining pure algae colonies from the algae liquid obtained by domestication in the step (4) in a plate streaking mode, wherein the culture step is the same as the step (1), and after the culture is finished, selecting larger algae colonies for shake flask culture to obtain a target algae strain named as TMJ-D3.
Example 2 identification of algal strains
Extracting DNA of the obtained TMJ-D3 strain by CTAB method, cloning by 18S rDNA gene, and sequencing the obtained 3 positive clones by Shanghai' S chemical company. The sequencing analysis result of the 18S rDNA gene is shown in a sequence table. The 18S rDNA sequence is logged into a Genbank database for Blast comparison, and the result is displayed and compared withScenedesmus sp.The similarity is the greatest, the BLASTn value is 2562, the Max index value is 99.3 percent, and the TMJ-D3 can be determined as Scenedesmus obliquus (Scenedesmus obliquus)Scenedesmus sp.）。
Example 3 application of Scenedesmus TMJ-D3
Inoculating TMJ-D3 algae solution in logarithmic phase into BG11 culture medium for culture, wherein the formula of BG11 culture medium is shown in tables 1 and 2, culturing in a photobioreactor, and inoculating OD of the culture medium690Is 0.25. Introducing CO from the bottom of the reactor230v% of flue gas, wherein NO content is 0.08 v%. In the culture process, the illumination intensity is 8000Lux, the culture temperature is 35 ℃, the pH value is controlled at 7-8, the illumination period is 24h, the light-dark time ratio is 14:10, and the culture time is 8 days and is in a stable period. And (3) finishing the culture, centrifugally collecting algae liquid, carrying out vacuum freeze drying at the temperature of-60 ℃ to constant weight, measuring the dry weight of algae powder, calculating the biomass yield, and adopting normal hexane: the total lipid content was determined by ethyl acetate method. The biomass yield of the TMJ-D3 strain is 10.5g/L, and the total lipid content of the cells accounts for 42.89% of the dry weight of the cells.
TABLE 1 BG11 culture Medium
Table 2 composition of a5+ Co solution in table 1
Example 4 comparison of the culture Effect of TMJ-D3 and TMJ-3
Inoculating strain solutions of TMJ-D3 and TMJ-3 in logarithmic phase into BG11 culture medium, culturing in photobioreactor, and inoculating OD of the culture solution690Is 0.25. According to the test requirements, the CO with different contents is prepared2And NO mixed gas is introduced from the bottom of the reactor. In the culture process, the illumination intensity is 8000Lux, the temperature is 35 ℃, the pH value is controlled between 7 and 8, the illumination period is 24 hours, the light-dark time ratio is 14:10, the culture time is 8 days, the algae cells are collected after the culture is finished, and the dry weight of the cells is measured. The results are shown in Table 3.
TABLE 3 comparison of the culture Effect of TMJ-D3 and TMJ-3
As can be seen from Table 3, the TMJ-D3 bred by the present invention has better tolerance to NO than the original strain TMJ-3. Meanwhile, the removal rate can reach more than 80 percent through detecting the content of NO in the exhaust gas.
Example 5 comparison of culture temperatures of TMJ-D3 and TMJ-3
Inoculating the strain solutions of TMJ-D3 and TMJ-3 in logarithmic phase into BG11 culture medium, culturing in a photobioreactor, and determining OD of the inoculated culture solution690Is 0.25. Introducing CO from the bottom of the reactor210v% of flue gas, wherein the NO content is 0.02. In the culture process, the illumination intensity is 8000Lux, the pH value is controlled to be 7-8, the illumination period is 24h, the light-dark time ratio is 14:10, and the culture time is 8 days. After the completion of the culture, algal cells were collected and the dry weight of the cells was measured, and the results are shown in Table 4.
TABLE 4 comparison of culture results of TMJ-D3 and TMJ-3
As can be seen from Table 4, TMJ-D3 of the present invention has better high temperature resistance than the original strain TMJ-3, but neither strain tolerates low temperatures.
Comparative example 1
The difference from example 3 is that Scenedesmus MH-04 (disclosed in CN 106467897A)Desmodesmus sp.) The preservation number is CGMCC No.10764, and the culture is finished for 8 days. The biomass yield of the MH-04 algal strain is detected to be 3.2g/L, and the total lipid content of the cells accounts for 33.5 percent of the dry weight of the cells.
Comparative example 2
The difference from example 3 is that Scenedesmus algae HCS-02 (disclosed in CN 107177505A)Scenedesmus sp.) The preservation number is CGMCC No.10766, and the culture is finished for 8 days. Through detection, the biomass yield of the HCS-02 algal strain is 2.1g/L, and the total lipid content of the cells accounts for 30.56% of the dry weight of the cells.
Sequence listing
<110> China petrochemical Co., Ltd
China Petroleum & Chemical Corporation Dalian Petrochemical Research Institute
<120> oil-producing scenedesmus and culture application thereof
<130> New patent application
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1420
<212> DNA
<213> Scenedesmus sp.
<400> 1
gtagtcatat gcttgtctca aagattaagc catgcatgtc taagtataaa ctgcttatac 60
tgtgaaactg cgaatggctc attaaatcag ttatagttta tttggtggta ccttcttact 120
cggaataacc gtaagaaaat tagagctaat acgtgcgtaa atcccgactt ctggaaggga 180
cgtatatatt agataaaagg ccgaccggac tttgtccgac ccgcggtgaa tcatgatatc 240
ttcacgaagc gcatggcctt gtgccggcgc tgttccattc aaatttctgc cctatcaact 300
ttcgatggta ggatgatctg tagccatggt ggtaacgggt gacggaggat tagggttcga 360
ttccggagag ggagcctgag aaacggctac cacatccaag gaaggcagca ggcgcgcaaa 420
ttacccaatc ctgatacggg gaggtagtga caataaataa caataccggg cattttatgt 480
ctggtaattg gaatgagtac aatctaaatc ccttaacgag gatccattgg agggcaagtc 540
tggtgccagc agccgcggta attccagctc caatagcgta tatttaagtt gttgcagtta 600
aaaagctcgt agttggattt cgggtgggtt tcagcggtcc gcctatggtg agcactgctg 660
tggccttcct tactgtcggg gacctgcttc tgggcttcat tgtccgggac agggattcgg 720
catggttact ttgagtaaat tagagtgttc aaagcaggct tacgccgtga atactttagc 780
atggaataac acgataggac tctgccctat tctgttggcc tgtaggagtg gagtaatgat 840
taagaggaac agtcgggggc attcgtattt cattgtcaga ggtgaaattc ttggatttat 900
gaaagacgaa ctactgcgaa agcatttgcc aaggatgttt tcattaatca agaacgaaag 960
ttgggggctc gaagacgatt agataccgtc gtagtctcaa ccataaacga tgccgactag 1020
ggattggcgg acgtttttgc atgactccgt cagcaccttg agagaaatca aagtttttgg 1080
gtttcggggg gagtatggtc gcaaggctga aacttaaagg aattgacgga agggcaccac 1140
caggcgtgga gccgacggct taatttgact caacacggga aaacttacca ggtccagaca 1200
taggaaggat tgacagattg agagctcttt cttgattcta tgggtggtgg tgcatggccg 1260
ttcttagttg gtgggttgtc ttgtcaggtt gattccggta acgaacgaga cctcagcctt 1320
taaatagtca cagtcgcttt ttgcgggtgg tttgacttct tagagggaca gttggcgttt 1380
agtcaacgga agtatgaggc aataacaggt ctagtgatta 1420
Claims (10)
1. Scenedesmus for producing oil, the Scenedesmus is a Scenedesmus for producing oilAlgae TMJ-D3, its classification name is Scenedesmus obliquus (Scenedesmus obliquus)Scenedesmussp.) And the strain is preserved in China general microbiological culture Collection center (CGMCC) at 2018, 2 and 5, with the preservation number of CGMCC 15299.
2. The oil producing scenedesmus of claim 1, wherein: under a microscope, algal cells of the algal strain are green and are often aggregated into a group, the group consists of four cells, and the cells of the group are arranged in a straight line; the single cell is long oval, contains chromosome, and contains one protein nucleus, and has a length of about 5-7 μm and a width of about 2-3 μm; the upper and lower ends of the cells on both sides of the colony are respectively provided with a long or straight or slightly bent prick, and both ends of the cells in the middle part and the side free parts of the cells on both sides are provided with no prick; the width of the four-cell population is 15-30 microns, and the puncture length is 10-15 microns.
3. The oil producing scenedesmus of claim 1, wherein: the strain can tolerate CO2Up to 30v%, and can tolerate NOx concentrations up to 0.08 v%.
4. The oil producing scenedesmus of claim 1, wherein: the strain can endure high temperature, can grow normally at 40-45 ℃, and keeps high biomass.
5. The oil producing scenedesmus of claim 1 immobilized CO2The use of (1).
6. Use of Scenedesmus olea according to claim 1 for the production of microalgal oil.
7. The method of claim 1 for purifying oil-producing scenedesmus containing CO2And in NOx exhaust or flue gases.
8. The method for culturing Scenedesmus oleaginous according to claim 1, comprising: introducing CO into photobioreactor with BG11 culture medium or SE culture medium2The content is 1.0v%30v% of gas was used for the culture.
9. The culture method according to claim 8, wherein: the gas contains NOx, and the concentration of NOx is not higher than 0.08 v%.
10. The culture method according to claim 8 or 9, wherein: the culture conditions were: the illumination intensity is 1500-.
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| CN117327587A (en) * | 2023-11-20 | 2024-01-02 | 广东能源集团科学技术研究院有限公司 | Scenedesmus and application thereof |
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Effective date of registration: 20231129 Address after: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec (Dalian) Petrochemical Research Institute Co.,Ltd. Address before: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: DALIAN RESEARCH INSTITUTE OF PETROLEUM AND PETROCHEMICALS, SINOPEC Corp. |