CN107460216B - Method for producing microalgae grease by using flue gas - Google Patents

Method for producing microalgae grease by using flue gas Download PDF

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CN107460216B
CN107460216B CN201610386730.9A CN201610386730A CN107460216B CN 107460216 B CN107460216 B CN 107460216B CN 201610386730 A CN201610386730 A CN 201610386730A CN 107460216 B CN107460216 B CN 107460216B
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microalgae
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scenedesmus
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师文静
王鹏翔
廖莎
孙启梅
李晓姝
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China Petroleum and Chemical Corp
Sinopec Fushun Research Institute of Petroleum and Petrochemicals
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    • C—CHEMISTRY; METALLURGY
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    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract

The invention discloses a method for producing microalgae grease by using smoke, which comprises the steps of (1) adding a microalgae culture medium and a Chlorella Kelvin FSH-Y3 seed liquid into a photobioreactor, adjusting the pH of the culture system to 10-12, and introducing CO2Culturing for 2-5 days by using smoke with the volume content of 1-5 v%; (2) adjusting the pH value of the culture system to 8-10, and inoculating Scenedesmus obliquus (Scenedesmus obliquus) (A)Desmodesmus sp.) MH-04, Mono-needled algae (MonorapHidium sp) One or two of SS-B1 seed liquids are inoculated with fibroalgae (A), (B), (C)Ankistrodesmus sp.) Mixed culture is carried out on SS-B7 seed liquid, and CO is introduced2And (3) culturing the smoke with the volume content of 5-45 v% to a stable period under the condition of continuous illumination, and harvesting the microalgae cells. The method improves the microalgae culture system to high-concentration CO2The tolerance and the solubility of the microalgae oil improve the carbon fixation efficiency, improve the tolerance to SOx and NOx in the flue gas, and obviously improve the yield of the microalgae oil.

Description

Method for producing microalgae grease by using flue gas
Technical Field
The invention belongs to the field of biotechnology and biological energy, and particularly relates to a method for producing microalgae grease by using flue gas.
Background
as fossil energy is decreasing and the greenhouse effect is increasing due to the use of fossil energy, more and more researchers are focusing on the development and utilization of renewable energy. Biomass can be the most important renewable energy on earth, and comprises forestry biomass, crops, aquatic plants, agricultural wastes and the like. Among the many biomass energy sources, microalgae are important renewable resources. They have the characteristics of wide distribution, large biomass, high photosynthesis efficiency, strong environment adaptability, short growth period, high biomass yield and the like. The cells contain unique primary or secondary metabolites and are chemically complex. The solar energy conversion efficiency of the microalgae can reach 3.5 percent, the microalgae is a potential resource for producing medicines, fine chemicals and novel fuels, and fatty acid obtained from the microalgae can be converted into fatty acid methyl ester, namely biodiesel.
With the development of the world economy, the use and consumption of a large amount of fossil energy, resulting in the shortage of energy and the increasing deterioration of the environment, particularly CO2The greenhouse effect is getting more and more serious due to the sharp increase of the amount of the organic compound. Short growth period of microalgae, high photosynthetic efficiency, and CO2High fixing efficiency which is more than 10 times of that of terrestrial plants under certain conditions, and can reduce CO2The discharge and the culture cost are reduced; CO removal2Besides, some components such as SOx, NOx and the like in the exhaust gas are purified along with the metabolism of microalgae, so that the emission of harmful gases is effectively reduced, and therefore, the biodiesel produced by using the microalgae grease as a raw material is a renewable energy source which is most likely to meet the requirements of fuels required by world transportation at present.
At present, more researches are carried out on oil-producing microalgae such as chlorella and scenedesmus. CN20110144545.6 discloses a Scenedesmus algae strain, which can grow by using artificial culture medium or properly treated waste water, and is characterized by that its oil yield is higher than that of most of present algae-separating strains, and its application field includes CO2The fixation, the purification of waste water, and the production of grease, protein, pigment, starch, polysaccharide and nucleic acid. CN20120154470.4 discloses a nannochloropsis oculata strain of oil-rich marine microalgae (nannochloropsis oculata: (Nannochloropsis gaditana ) The algae strain can normally grow under the environment that the pH =4.5, and the oil content of the algae strain can reach 35%. CN20111019480.X discloses a microalgae strainMychonases sp.) And application of the strain in producing biodiesel, and the strain can be used for producing biodiesel with high added valuePolyunsaturated fatty acids, including linolenic acid C18:3 and nervonic acid C24:1, yield high value-added by-products while simultaneously yielding biodiesel. CN102703326A discloses a high CO2The tolerance and the fixed rate of the microalgae and the breeding method thereof, but the algae strain provided by the patent does not relate to the oil content of the algae strain. The above patents either cannot utilize CO efficiently2Oil is produced, or the content of oil in the obtained biomass is not high enough. Especially in practical applications when CO is present2at concentrations above 5%, the growth of most microalgae will be inhibited, and the CO in the gas discharged industrially will be2The concentration is generally 10% -20%, and the microalgae also contains substances with toxic effects on the microalgae, such as SOx, NOx and the like. Therefore, for direct fixation of CO in industrial exhaust gases2In addition to the requirement for CO2Has high conversion rate, fast growth rate, wide pH tolerance range and high CO tolerance2Concentration and tolerance of harmful substances such as SOx, NOx and the like.
Liu Ping Huai et al (organic carbon source influences growth, oil accumulation and photosynthesis of the monoraphidium, bioengineering, 2012, 33(18): 224-246) introduce a production mode for culturing the monoraphidium by using the organic carbon source, the biomass exceeds 10g/L after the culture is finished, the mode is a heterotrophic culture mode for the monoraphidium, the organic carbon source such as glucose is used for realizing the cell growth in the culture process, and CO is not used in the culture mode2And the addition of the organic carbon source is easy to cause the problem of bacterial contamination in the culture process and influence the growth of algae cells.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for producing microalgae grease by using flue gas. The method improves the microalgae culture system to high-concentration CO2The tolerance and the solubility of the microalgae oil improve the carbon fixation efficiency, improve the tolerance to SOx and NOx in the flue gas, and obviously improve the yield of the microalgae oil.
The method for producing the microalgae grease by using the flue gas comprises the following steps:
(1) Mixing microalgae culture medium with Chlorella KelvinAdding the FSH-Y3 seed liquid into a photobioreactor, adjusting the pH of a culture system to 10-12, and introducing CO2Culturing for 2-5 days by using smoke with the volume content of 1-5 v%;
(2) Adjusting the pH value of the culture system to 8-10, and inoculating Scenedesmus obliquus (Scenedesmus obliquus) (A)Desmodesmus sp.) MH-04 seed liquid, Mono-needled algae (MH-04 seed liquid)MonorapHidium sp) One or two of SS-B1 seed liquids are inoculated with fibroalgae (A), (B), (C)Ankistrodesmus sp.) Mixed culture is carried out on SS-B7 seed liquid, and CO is introduced2Culturing smoke with the volume content of 5-45 v% to a stable period under the condition of continuous illumination, and harvesting microalgae cells;
Wherein said Chlorella chekiangensis is selected from (A), (B), (CParachlorella kessleri) FSH-Y3, Scenedesmus obliquus (Scenedesmus obliquus)Desmodesmus sp.) MH-04, Mono-needled algae (MonorapHidium sp) SS-B1 and Cellulosium (A)Ankistrodesmus sp.) SS-B7, which is respectively preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms (CGMCC), with the preservation numbers of CGMCC No. 9238, CGMCC No. 10764, CGMCC No. 7479 and CGMCC No.7478, at 26 days 5 and 24 days 2015 and 15 days 2013 and 4 and 15 days 2013, and the preservation addresses are the institute for microbiology of China academy of sciences No. 3, North Cheng West Lu 1, the sunward area of Beijing. Wherein the algae of Dinophyta (MonorapHidium sp) SS-B1 is an algal strain disclosed in CN 201310537912.8.
In the invention, the chlorella cathelii FSH-Y3 algal strain has spherical and elliptical algal cells under a microscope, and a periphytic, cup-shaped or sheet-shaped chromosome is arranged in the algal cells; asexual propagation, each cell can produce 2, 4, 8 or 16 similar parent spores, the blast cell is broken when the blast cell is mature, the spores are escaped, and the grown-up blast cell is a new individual. The algal strain can better absorb and utilize carbon dioxide under a high pH value, and can quickly grow and propagate. The Scenedesmus MH-04 strain is a freshwater green alga, algae cells are green under a microscope and are often aggregated into a group, single cells are oval, cell walls are smooth, endosomes are provided, each cell contains a protein nucleus, the length of each single cell is about 5-6 mu m, and the width of each single cell is about 2-3 mu m. The unicladus SS-B1 strain is a freshwater green algae, the algae cell is long-leaf-shaped and green, the algae seed is 10-20 μm long and 2-4 μm wide, and the algae seed containsPigment, dull algae colony shape is S-shaped, dark green. Scenedesmus MH-04 and Monochoria SS-B1 are able to tolerate high concentrations of CO2And SO2Can use CO-containing2And SO2The waste gas or the smoke is subjected to illumination autotrophic growth to obtain biomass rich in grease, and the carbon fixation efficiency is high. The Cellulomonas SS-B7 strain is a fresh water green algae, and under microscope, the algae cell is green, sickle-shaped or bow-shaped, clustered, bent, tapered from the center to the two ends, and has a tip end, a length of about 5-6 μm, and a width of about 2 μm at the center. The strain can tolerate high concentration of CO2And NOx, can utilize CO-containing2And the waste gas or the smoke of NOx is subjected to illumination autotrophic growth to obtain the biomass rich in grease, so that the carbon sequestration efficiency is high.
In the present invention, as the microalgae culture medium, a liquid culture medium for culturing microalgae, such as BG11, SE, or BBM, which is well known to those skilled in the art, is used.
In the invention, the preparation of the Chlorella Kelvin FSH-Y3 seed liquid is as follows: adjusting the pH value of the culture medium to 10-12, and carrying out shake culture to logarithmic phase under the conditions that the temperature is 20-30 ℃, the illumination period is 24 hours, the light-dark time ratio is 14:10, and the illumination intensity is 2000-20000 Lux. Preferably, Scenedesmus obliquus (Scenedesmus obliquus) described in CN201310537896.2 is added simultaneouslyScenedesmus obliqnus) FSH-Y2 seed liquid, wherein the strain is preserved in 'China general microbiological culture Collection center' on 9/11/2012, with the preservation number of CGMCC 6551 and the preservation address of China academy of sciences microbial research institute No. 3 of North West Lu No. 1 Homeh of the Chaozhou, Beijing. The preparation method of the Scenedesmus obliquus FSH-Y2 seed liquid is the same as that of Chlorella chekiangensis FSH-Y3, and the adding amount is that the volume ratio of the seed liquid to the culture medium is 1: 40-1: 10.
The preparation method of Scenedesmus MH-04 seed liquid and Monochoria SS-B1 seed liquid comprises the following steps: adjusting the pH value of a microalgae culture medium to 7-9, performing shaking culture at the temperature of 20-30 ℃, the illumination period of 24 hours, the light-dark time ratio of 14:10 and the illumination intensity of 2000-20000 Lux until the logarithmic growth phase.
In the invention, the fibroalgae SS-B7 seed solution is prepared as follows: adjusting the pH value of the culture medium to 7-9, performing shaking culture at the temperature of 20-30 ℃, the illumination period of 24h, the light-dark time ratio of 14:10 and the illumination intensity of 2000-10000 Lux until the logarithmic phase.
The total inoculation amount of the microalgae seed liquid is controlled to be 10-30% of the total volume of the culture medium, wherein the volume ratio of the Kjeldahl quasi-chlorella FSH-Y3 seed liquid, Scenedesmus MH-04 seed liquid and/or Scenedesmus SS-B1 seed liquid to the Cellulus SS-B7 seed liquid is 1:6: 6-4: 1: 1. When the Scenedesmus MH-04 seed liquid and the Monochoria SS-B1 seed liquid are simultaneously inoculated, the volume ratio of the Scenedesmus MH-04 seed liquid to the Monochoria SS-B1 seed liquid is 5:1-1: 5.
In the invention, the flue gas is derived from incineration tail gas of a sulfur recovery device, catalytic cracking regeneration tail gas or S-zorb regeneration tail gas, wherein CO is2The content of SO is 5-45 v%, and2The content is not more than 600 × 10-6(v/v) NOx content of not more than 500X 10-6（v/v）。
In the invention, the temperature of mixed culture is 20-30 ℃, the illumination period is 24h, the light-dark time ratio is 14:10, the illumination intensity is 2000-10000 Lux, and the culture is carried out until the growth stabilization period is finished. Microalgae cells are harvested by centrifuging, settling and other modes, the dry weight of the cells and the oil content are measured, the dry weight of the cells can reach more than 12g/L, the biomass yield of the cells reaches 1.5 g/(L.d), the oil content can reach more than 45% of the dry weight of the cells, the removal rate of carbon dioxide is increased to more than 50%, and the removal rate of NOx can reach 20%.
Compared with the prior art, the invention can bring the following beneficial effects:
1. firstly, performing single culture by adopting the Chlorella Kelvin FSH-Y3 which can tolerate a high-pH environment, wherein the initial high-pH and intermittent illumination can inhibit the growth of mixed bacteria and plant diseases and insect pests at the initial stage of microalgae culture, and are beneficial to the growth advantage of microalgae; and high pH favors CO2By dissolving of CO2Is easier to be absorbed and utilized by the microalgae, and is helpful for improving CO2The efficiency of the fixation.
2. After 2-5 days of culture, the pH is lowered and SO in the tolerant flue gas is added2And the continuous illumination culture of the microalgae with NOx is beneficial to promoting the rapid growth of the microalgae and improving the growth rate of the microalgae. Moreover, the algae can be matched with each other, and have higher carbon fixing rate than the culture of single algae speciesThe efficiency and the carbon dioxide removal rate are higher, and the obtained biomass contains more grease content.
3. The mixed culture system of the invention can tolerate CO with high concentration2、SO2And NOx, CO in the exhaust gas can be utilized2Autotrophic growth and CO fixation2And the problems of greenhouse effect and waste gas pollution brought by the current industrial society are solved.
Detailed Description
The present invention will be described in further detail by way of examples. In the present invention, wt% is a mass fraction and v% is a volume fraction. Wherein the biomass yield is the mass g/(volume L of algae solution) of the harvested algae powder and the removal rate is (gas content-exhaust gas content)/gas content. The culture of the microalgae adopts BG11 culture medium, and the formula of the culture medium is shown in tables 1 and 2.
TABLE 1 BG11 culture Medium
Table 2 composition of a5+ Co solution in table 1
BG11 liquid medium was prepared according to tables 1 and 2, the pH of the medium for culturing the Chlorella Kelvin FSH-Y3 and Scenedesmus obliquus FSH-Y2 was adjusted to 10, the pH of the medium for culturing Scenedesmus obliquus MH-04, Scenedesmus obliquus SS-B1 and Scenedesmus fibrosus SS-B7 was adjusted to 8.0, and then the culture media were inoculated with the respective Chlorella Kelvin FSH-Y3, Scenedesmus obliquus FSH-Y2, Scenedesmus obliquus MH-04, Scenedesmus obliquus SS-B1 and Chlorella fibrosus SS-B7. Culturing in constant temperature light shaking table at 25 deg.C with illumination period of 24h, light-dark time ratio of 14:10 and illumination intensity of 5000Lux at 120rpm, shaking and culturing to logarithmic growth phase to obtain Chlorella Kelvin FSH-Y3 seed solution, Scenedesmus obliquus FSH-Y2 seed solution, Scenedesmus obliquus MH-04 seed solution, Monofilica SS-B1 seed solution and Cellulus SS-B7 seed solution, and storing the seed solutions at 15 deg.C under weak light for use.
Example 1
(1) Adding the Chlorella Kelly FSH-Y3 seed liquid prepared in example 1 and a microalgae culture medium into a 10L photobioreactor, wherein the seed liquid is added in an amount of 400mL, the pH value of the microalgae culture medium is adjusted to 10, the addition amount is 8L, the illumination intensity of the culture is 5000Lux, the illumination period is 24h, the light-dark time ratio is 14:10, introducing CO in smoke2In an amount of 3v%, NO and NO2The content is 50X 10-6（v/v），SO2The content is 60 multiplied by 10-6（v/v）。
(2) After culturing for 4 days, inoculating 400mL of scenedesmus MH-04 seed solution prepared in example 1 and 400mL of fibrophyta SS-B7 seed solution, adjusting the pH of the microalgae culture system to 8, and continuously culturing by illumination with the illumination intensity of 5000 Lux; introducing CO into the flue gas2In an amount of 30v%, NO and NO2The content is 500X 10-6（v/v），SO2The content is 600 multiplied by 10-6（v/v）。
(3) And (4) after culturing for 7 days, entering a growth stabilization phase, finishing culturing, centrifugally harvesting microalgae cells, and measuring the dry weight and the oil content of the cells. And (3) carrying out vacuum freeze drying at the temperature of-60 ℃ to constant weight, measuring the dry weight of the algae powder, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. After detection, the dry weight of the cells can reach 12.7g/L, the oil content is 45.7 percent of the dry weight of the cells, and CO is generated in the culture process2The removal rate was 51%, and the NOx removal rate was 21.3%.
Example 2
(1) Adding the Kjeldahl quasi-globule FSH-Y3 seed liquid prepared in the example 1 and a microalgae culture medium into a 10L photobioreactor, wherein the addition amount of the seed liquid is 800mL, the pH value of the microalgae culture medium is adjusted to be 12, the addition amount is 8L, the illumination intensity of the culture is 5000Lux, the illumination period is 24h, the light-dark time ratio is 14:10, introducing CO in smoke2In an amount of 5v%, NO and NO2The content is 80X 10-6（v/v），SO2The content is 100 x 10-6（v/v）。
(2) After culturing for 2 days, 560mL of Scenedesmus MH-04 seed solution and 560mL of Cellulomonas SS-B7 seed solution prepared in example 1 were inoculated to adjust microalgae cultureThe pH of the system is 9, the continuous illumination culture is carried out, and the illumination intensity is 5000 Lux; introducing CO into the flue gas2In an amount of 30v%, NO and NO2The content is 500X 10-6（v/v），SO2The content is 600 multiplied by 10-6（v/v）。
(3) After culturing for 8 days, entering a stable period, finishing culturing, centrifugally harvesting microalgae cells, and measuring the dry weight and the oil content of the cells. And (3) carrying out vacuum freeze drying at the temperature of-60 ℃ to constant weight, measuring the dry weight of the algae powder, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. The dry weight of the cells can reach 12.5g/L, the oil content is 46.1 percent of the dry weight of the cells, and CO is generated in the culture process2The removal rate was 50.4%, and the NOx removal rate was 21.9%.
Example 3
(1) Adding the Kjeldahl quasi-globule FSH-Y3 seed liquid prepared in the example 1 and a microalgae culture medium into a 10L photobioreactor, wherein the addition amount of the seed liquid is 800mL, the pH value of the microalgae culture medium is adjusted to be 12, the addition amount is 8L, the illumination intensity of the culture is 5000Lux, the illumination period is 24h, the light-dark time ratio is 14:10, introducing CO in smoke2In an amount of 5v%, NO and NO2The content is 80X 10-6（v/v），SO2The content is 100 x 10-6（v/v）。
(2) After 2 days of culture, inoculating 560mL of the Mono-needled algae SS-B1 seed solution prepared in example 1 and 560mL of the fibrophyta SS-B7 seed solution, adjusting the pH of the microalgae culture system to 9, and continuously culturing by illumination with the illumination intensity of 5000 Lux; introducing CO into the flue gas2In an amount of 30v%, NO and NO2the content is 500X 10-6（v/v），SO2The content is 600 multiplied by 10-6（v/v）。
(3) After culturing for 8 days, entering a stable period, finishing culturing, centrifugally harvesting microalgae cells, and measuring the dry weight and the oil content of the cells. And (3) carrying out vacuum freeze drying at the temperature of-60 ℃ to constant weight, measuring the dry weight of the algae powder, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. The dry weight of the cells can reach 12.1g/L, the oil content is 45.6 percent of the dry weight of the cells, and CO is generated in the culture process2The removal rate was 50.2%, and the NOx removal rate was 20.8%.
Example 4
(1) Adding the Kjeldahl quasi-globule FSH-Y3 seed liquid prepared in the example 1 and a microalgae culture medium into a 10-light bioreactor, wherein the addition amount of the seed liquid is 800mL, the pH value of the microalgae culture medium is adjusted to be 12, the addition amount is 8L, the illumination intensity of the culture is 5000Lux, the illumination period is 24h, the light-dark time ratio is 14:10, introducing CO in smoke2In an amount of 5v%, NO and NO2The content is 80X 10-6（v/v），SO2The content is 100 x 10-6（v/v）。
(2) After culturing for 2 days, inoculating 280mL of Scenedesmus MH-04 seed solution, 280mL of Monochorus SS-B1 seed solution and 560mL of fibrophyta SS-B7 seed solution prepared in example 1, adjusting the pH of a microalgae culture system to 9, and continuously culturing by illumination with the illumination intensity of 5000 Lux; introducing CO into the flue gas2In an amount of 30v%, NO and NO2The content is 500X 10-6（v/v），SO2The content is 600 multiplied by 10-6（v/v）。
(3) After culturing for 8 days, entering a stable period, finishing culturing, centrifugally harvesting microalgae cells, and measuring the dry weight and the oil content of the cells. And (3) carrying out vacuum freeze drying at the temperature of-60 ℃ to constant weight, measuring the dry weight of the algae powder, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. The dry weight of the cells can reach 13.2g/L, the oil content is 46.9 percent of the dry weight of the cells, and CO is generated in the culture process2The removal rate was 52.7%, and the NOx removal rate was 21.1%.
Example 5
The same culture procedure and culture conditions as in example 1 were used, except that: 200mL of Chlorella Kelvin FSH-Y3 seed solution and 200mL of Scenedesmus obliquus FSH-Y2 seed solution are added in the step (1). The dry weight of the cells can reach 13.1g/L, the oil content is 47.1 percent of the dry weight of the cells, and CO is generated in the culture process2The removal rate was 51.9%, and the NOx removal rate was 21.9%.
Example 6
The same culture procedure and culture conditions as in example 3 were used, except that: 400mL of the Chlorella Kelvin FSH-Y3 seed solution and 400mL of the Scenedesmus obliquus FSH-Y2 seed solution are added in the step (2). The dry weight of cells can reach 128g/L, oil content 47.1% of cell dry weight, CO in culture process2The removal rate was 50.9%, and the NOx removal rate was 22.9%.
comparative example 1
The same culture procedure and culture conditions as in example 1 were used, except that: FSH-Y3 seed solution, Scenedesmus MH-04 seed solution, and Cellulosium SS-B7 seed solution were added to the reactor together at the start of culture. And (3) after the culture is finished, centrifuging to obtain microalgae cells, and measuring the dry weight of the cells and the oil content, wherein the dry weight of the cells can reach 11.7g/L, and the oil content is 44.1% of the dry weight of the cells.
Comparative example 2
The same culture procedure and culture conditions as in example 1 were used, except that: adding 800mL of FSH-Y3 seed solution, culturing for 4 days, and changing the culture conditions in the step (2) without adding other microalgae. And finishing the culture after 7 days of culture, centrifuging to obtain microalgae cells, and measuring the dry weight and the oil content of the cells. The dry cell weight can reach 3.9g/L, and the oil content is 36.6% of the dry cell weight.
Comparative example 3
The same culture procedure and culture conditions as in example 1 were used, except that: in the step (1), 800mL of MH-04 seed solution is added, and the pH value of the culture medium is always 8. And finishing the culture after 11 days of culture, centrifuging to obtain microalgae cells, and measuring the dry weight and the oil content of the cells. The dry cell weight can reach 6.9g/L, and the oil content is 39.8% of the dry cell weight.
Comparative example 4
The same culture procedure and culture conditions as in example 1 were used, except that: 800mL of fibroalgae SS-B7 seed solution is added in the step (1), and the pH value of the culture medium is always 8. And finishing the culture after 11 days of culture, centrifuging to obtain microalgae cells, and measuring the dry weight and the oil content of the cells. The dry cell weight can reach 10.2g/L, and the oil content is 41.9% of the dry cell weight.
In conclusion, compared with a single algae species, the Chlorella Kelvin FSH-Y3, Scenedesmus MH-04, Mono-needle algae SS-B1 and Cellulomonas Fibrosus SS-B7 adopt two-step mixed culture, which is beneficial to improving the tolerance capability of a culture system and can obtain higher biomass and oil content. The invention utilizes the flue gas to prepare the microalgae grease, thereby realizing the production of the grease, purifying the waste gas and obviously improving the economic benefit and the environmental benefit.

Claims (11)

1. A method for producing microalgae grease by using flue gas is characterized by comprising the following steps: (1) adding a microalgae culture medium and a Chlorella Kelvinea FSH-Y3 seed solution into a photobioreactor, adjusting the pH of a culture system to 10-12, and introducing CO2Culturing for 2-5 days by using smoke with the volume content of 1-5%; (2) adjusting the pH value of the culture system to 8-10, and inoculating Scenedesmus obliquus (Scenedesmus obliquus) (A)Desmodesmus sp.) MH-04 seed liquid, Mono-needled algae (MH-04 seed liquid)MonorapHidium sp) One or two of SS-B1 seed liquids are inoculated with fibroalgae (A), (B), (C)Ankistrodesmus sp.) Mixed culture is carried out on SS-B7 seed liquid, and CO is introduced2Culturing smoke with volume content of 5-45% under continuous illumination condition to a stable stage, and harvesting microalgae cells; wherein said Chlorella chekiangensis is selected from (A), (B), (CParachlorella kessleri) FSH-Y3, Scenedesmus obliquus (Scenedesmus obliquus)Desmodesmus sp.) MH-04, Mono-needled algae (MonorapHidium sp) SS-B1 and Cellulosium (A)Ankistrodesmus sp.) SS-B7, which is preserved in China general microbiological culture Collection center (CGMCC) in 26 days 5 and 26 days 2015, 24 days 4 and 24 days 2013 and 15 days 4, and the preservation numbers are CGMCC 9238, CGMCC 10764, CGMCC 7479 and CGMCC No.7478 respectively.
2. The method of claim 1, wherein: the microalgae culture medium adopts BG11 culture medium, SE culture medium or BBM culture medium.
3. The method of claim 1, wherein: the preparation of the Chlorella Kelvin FSH-Y3 seed liquid is as follows: adjusting the pH value of the culture medium to 10-12, and carrying out shake culture to logarithmic phase under the conditions that the temperature is 20-30 ℃, the illumination period is 24 hours, the light-dark time ratio is 14:10, and the illumination intensity is 2000-20000 Lux.
4. A method according to claim 1 or 3The method is characterized in that: the scenedesmus obliquus is added in the step (1) at the same timeScenedesmus obliqnus) FSH-Y2 seed liquid, wherein the preservation number of Scenedesmus obliquus FSH-Y2 is CGMCC number 6551; the preparation method of the Scenedesmus obliquus FSH-Y2 seed liquid is the same as that of Chlorella chekiangensis FSH-Y3, and the adding amount is that the volume ratio of the seed liquid to the culture medium is 1: 40-1: 10.
5. The method of claim 1, wherein: the preparation method of Scenedesmus MH-04 seed liquid and Monochorus SS-B1 seed liquid comprises the following steps: adjusting the pH value of a microalgae culture medium to 7-9, performing shaking culture at the temperature of 20-30 ℃, the illumination period of 24 hours, the light-dark time ratio of 14:10 and the illumination intensity of 2000-20000 Lux until the logarithmic growth phase.
6. The method of claim 1, wherein: the preparation of the seed solution of the fibroalgae SS-B7 is as follows: adjusting the pH value of the culture medium to 7-9, performing shaking culture at the temperature of 20-30 ℃, the illumination period of 24h, the light-dark time ratio of 14:10 and the illumination intensity of 2000-10000 Lux until the logarithmic phase.
7. The method of claim 1, wherein: the total inoculation amount of the microalgae seed liquid is controlled to be 5-30% of the total volume of the culture medium, wherein the volume ratio of the Chlorella Kelvinei FSH-Y3 seed liquid, Scenedesmus MH-04 seed liquid and/or Monostroma SS-B1 seed liquid to the Cellulus SS-B7 seed liquid is 1:6: 6-4: 1: 1.
8. The method of claim 1 or 7, wherein: when the Scenedesmus MH-04 seed liquid and the Monochoria SS-B1 seed liquid are simultaneously inoculated, the volume ratio of the Scenedesmus MH-04 seed liquid to the Monochoria SS-B1 seed liquid is 5:1-1: 5.
9. The method of claim 1, wherein: the flue gas is derived from incineration tail gas of a sulfur recovery device, catalytic cracking regeneration tail gas or S-zorb regeneration tail gas.
10. A method according to claim 1 or 9, characterized in thatIn the following steps: CO in the flue gas2The content of SO is 5 to 45 percent (v/v)2The content is not more than 0.06% (v/v), and the NOx content is not more than 0.05% (v/v).
11. The method of claim 1, wherein: the temperature of microalgae mixed culture is 20-30 ℃, the illumination period is 24h, the light-dark time ratio is 14:10, the illumination intensity is 2000-10000 Lux, and the culture is completed until the growth stabilization period is finished.
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