CN114507603B - Method for open culture of oleaginous microalgae - Google Patents
Method for open culture of oleaginous microalgae Download PDFInfo
- Publication number
- CN114507603B CN114507603B CN202011169520.7A CN202011169520A CN114507603B CN 114507603 B CN114507603 B CN 114507603B CN 202011169520 A CN202011169520 A CN 202011169520A CN 114507603 B CN114507603 B CN 114507603B
- Authority
- CN
- China
- Prior art keywords
- microalgae
- culture
- gas
- dark
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000005286 illumination Methods 0.000 claims abstract description 27
- 239000001963 growth medium Substances 0.000 claims abstract description 23
- 238000012258 culturing Methods 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 238000003306 harvesting Methods 0.000 claims abstract description 3
- 239000007789 gas Substances 0.000 claims description 21
- 238000004321 preservation Methods 0.000 claims description 16
- 241000195493 Cryptophyta Species 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 5
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical group [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 claims description 4
- 239000003546 flue gas Substances 0.000 claims description 4
- 241001301789 Desmodesmus abundans Species 0.000 claims description 3
- 239000002912 waste gas Substances 0.000 claims description 3
- 241000196169 Ankistrodesmus Species 0.000 claims description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 2
- 230000003698 anagen phase Effects 0.000 claims description 2
- 238000004523 catalytic cracking Methods 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims description 2
- 230000008929 regeneration Effects 0.000 claims description 2
- 238000011069 regeneration method Methods 0.000 claims description 2
- 229910052717 sulfur Inorganic materials 0.000 claims description 2
- 239000011593 sulfur Substances 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 239000004519 grease Substances 0.000 description 15
- 241000894006 Bacteria Species 0.000 description 13
- 238000001514 detection method Methods 0.000 description 11
- 230000012010 growth Effects 0.000 description 5
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000419 plant extract Substances 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 3
- 238000009629 microbiological culture Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 230000001651 autotrophic effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000195651 Chlorella sp. Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 240000000233 Melia azedarach Species 0.000 description 1
- 244000046146 Pueraria lobata Species 0.000 description 1
- 235000010575 Pueraria lobata Nutrition 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229940082880 azadirachta indica flower extract Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- OAYLNYINCPYISS-UHFFFAOYSA-N ethyl acetate;hexane Chemical compound CCCCCC.CCOC(C)=O OAYLNYINCPYISS-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for open culturing oleaginous microalgae, which comprises adding microalgae seed liquid and microalgae culture medium into an open photobioreactor, alternately culturing in light and dark, and introducing CO during photoreaction 2 Introducing CO during dark reaction 2 And NO X The illumination intensity is reduced after the gas is alternately cultured for a period of time, and the NO is stopped being introduced in the dark reaction X Culturing until the culture period is stable, and harvesting microalgae cells. The method solves the problem of mixed bacterial pollution in the open culture of microalgae by a specific culture mode, and reduces the culture cost.
Description
Technical Field
The invention belongs to the technical field of biomass energy, and particularly relates to a method for open-type culture of oleaginous microalgae.
Background
Microalgae is rich in protein, polysaccharide, unsaturated fatty acid and other nutritional components, and can be used in food, medicine and energy sources; can accumulate fatty acid in large quantity, and the content of the fatty acid of the microalgae can account for 30-60% of dry weight. The method for obtaining the grease resource by culturing the microalgae has become a popular research field for developing renewable resources by using solar energy, and has strong market potential and remarkable social value.
At present, microalgae culture modes are closed and open. The closed type refers to a closed type reactor with different structures, such as an airlift type reactor, a stirring type reactor, a tubular reactor and the like, has higher production cost, and can be used for producing products with high added value or used as a seed tank for open culture. Open-cell culture means such as a raceway pond and a round shallow pond are adopted, and the open-cell culture means has the advantages of simple technology and low investment, so that researchers are concerned in recent years. However, open cultures are susceptible to contamination by enemies such as filamentous fungi, rotifers, protozoa, etc., which grow and multiply in the algae culture. When the number of the enemy organisms reaches a certain density, the enemy organisms influence the growth and propagation of the cultured algae, the polluted algae liquid is unfavorable for the expansion of the culture, and the serious people can cause the failure of the culture. Therefore, how to effectively prevent the pollution of enemy organisms is a key problem for the large-scale cultivation of microalgae.
In recent years, many studies have been made on methods for controlling enemies such as microalgae pest and disease damage. The traditional method firstly adopts a physical method such as a filtering or acidification method to kill and eliminate enemy organisms in algae; secondly, controlling the pollution of enemy organisms in the open culture of the microalgae by a chemical method; thirdly, a biological control method is adopted for treatment; fourthly, the method of adding plant extracts is adopted for treatment.
CN103773690a discloses a method for culturing microalgae, the microalgae culture adopts conventional culture method and conditions, at the beginning of microalgae culture, plant extract is directly added into microalgae culture medium, and the plant extract is one or more of neem extract, szechwan chinaberry extract and kudzuvine root extract, and the addition amount is 5-80mg/L. The method can solve the problem of pest pollution in the open culture of microalgae, and effectively prevent and treat the pollution of infectious microbe and plant diseases and insect pests in the open culture. However, this method requires the specific preparation of a specific formulation of plant extract, which increases the cost of cultivation.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for open-type microalgae cultivation. The method solves the problem of mixed bacterial pollution in the open culture of microalgae by a specific culture mode, and reduces the culture cost.
The invention provides a method for open culturing microalgae, which comprises the following steps:
adding microalgae seed liquid and microalgae culture medium into an open type photobioreactor, performing alternate light and dark culture, and introducing CO during photoreaction 2 Introducing CO during dark reaction 2 And NO X The illumination intensity is reduced after the gas is alternately cultured for a period of time, and the NO is stopped being introduced in the dark reaction X Culturing until the culture period is stable, and harvesting microalgae cells.
In the invention, the microalgae can resist NO X The autotrophic single algae or mixed algae can be fibrous algae SS-B7%Ankistrodesmus sp.) SF-B1 of chlorellaChlorella sp.) HCY-BY1 of chain belt algaDesmodesmus abundans) At least one of the above components, preferably chain belt alga HCY-BY 1%Desmodesmus abundans）。
In the invention, the fibrous algae SS-B7 @ isAnkistrodesmus sp.) Has been preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 7478 and the preservation address of the national institute of microbiology, national academy of sciences No. 3, north Chenxi Lu No.1, the area of Chaoyang in Beijing, as well as in the 15 th 2013. This strain has been disclosed in CN105713836a and submitted for preservation and survival demonstration.
In the invention, the chlorella SF-B1 is%Chlorella sp.) Has been preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.11005 and the preservation address of China academy of China microbiological study (China academy of sciences) No. 3 of North Chenxi Lu No.1 in the Korean area of Beijing city at the date of 2015, 7 and 6. This strain has been disclosed in CN109576158A and submitted for preservation and survival demonstration.
In the invention, the chain belt alga HCY-BY is 1 #Desmodesmus abundans) Has been preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 19982 and the preservation address of North Chenxi road in the Korean region of Beijing city in the year of 5.12 of 2020Institute of microbiology, national academy of sciences, 1 and 3.
In the present invention, the microalgae culture medium is any one of BG11 culture medium, SE culture medium, BBM culture medium, etc., which are well known to those skilled in the art. Specifically, the preparation of the microalgae culture medium and the microalgae seed liquid is determined according to the types of the microalgae, and the preparation is the same as that of the conventional method.
In the invention, the volume ratio of the microalgae seed liquid to the microalgae culture medium added into the photobioreactor is 1:20-1:5.
In the invention, the preparation method of the microalgae seed liquid comprises the following steps: inoculating microalgae into a microalgae culture medium, and culturing in an oscillating manner until the growth phase of the microalgae seed liquid is logarithmic under the conditions that the pH value is 7-9, the temperature is 20-35 ℃, the illumination period is 24 hours, the light-dark time ratio is 14:10 and the illumination intensity is 2000-20000 Lux, thereby obtaining the microalgae seed liquid.
In the invention, the light and dark alternate culture is carried out at the culture temperature of 15-35 ℃, the illumination intensity of 2000-20000 Lux, the light and dark period of 24 hours and the light and dark time ratio of 14:10-10:14.
In the invention, the CO is introduced by photoreaction 2 In the gas, CO 2 The volume content is 5-40%, preferably 5-20%, and NO is contained X 。
In the invention, the dark reaction is introduced with CO 2 And NO X In the gas, CO 2 The volume content is 5-40%, NO X The volume content is 0.03-0.07%. Said NO X Is NO and/or NO 2 Said CO 2 And NO X The gas can be self-made, or can be flue gas or waste gas, such as at least one of incineration tail gas and catalytic cracking regeneration tail gas from a sulfur recovery device.
In the invention, the NO is stopped to be introduced after the alternate culture for 2 to 5 days X The illumination intensity is reduced by 20 to 50 percent on the basis of the original illumination, and the cultivation is finished.
In the invention, after the culture is finished, the number of the mixed bacteria is detected to be lower than 3.0x10 3 And each mL. Microalgae cells are harvested by centrifugation, sedimentation and other modes, and the dry weight and the grease content of the cells are measured.
Compared with the prior art, the invention has the following beneficial effects:
(1) The inventor of the application found in the research of open culture chain belt algae HCY-BY1 that the number of mixed bacteria in the finally obtained algae liquid is lower BY the culture mode of the invention, and on the basis, the inventor tolerates NO X Tests are carried out on the same time as the fibrous alga SS-B7 and the chlorella SF-B1, and the ideal effect can be obtained. On the basis, the culture method suitable for the microalgae is provided, the problem of mixed bacterial pollution in open culture of the microalgae is solved, the culture cost is reduced, and the method is suitable for large-scale culture.
(2) The culture mode of the invention is suitable for NO X The autotrophic microalgae with certain tolerance has no toxic or harmful effect on the growth of the microalgae, and can maintain the normal growth of the microalgae.
(3) The culture mode is particularly suitable for culturing the chain belt alga HCY-BY1, does not influence the normal growth of the alga strain, and is beneficial to the accumulation of microalgae grease. The detection shows that the dry weight of the cells can be increased by more than 10%, and the grease content can reach more than 50% of the dry weight of the cells.
Detailed Description
The invention is illustrated in further detail by the following examples. The embodiments and specific operation procedures are given on the premise of the technical scheme of the invention, but the protection scope of the invention is not limited to the following embodiments.
The experimental methods in the following examples, unless otherwise specified, are all conventional in the art. The experimental materials used in the examples described below, unless otherwise specified, were purchased from conventional biochemical reagent stores.
In the invention, CO in the gas 2 、NO X The concentration is detected by a flue gas analyzer.
The preservation number of the fibrous alga SS-B7 is CGMCC No. 7478, which is disclosed in CN105713836A and submitted for preservation and survival certification. The chlorella SF-B1 has a preservation number of CGMCC No.11005, is disclosed in CN109576158A, and submits preservation and survival evidence.
The chain belt alga HCY-BY1 is a new alga species separated and screened BY the inventor and is preserved in the common microorganism center of China Committee for culture Collection of microorganisms; preservation number: CGMCC No. 19982; preservation date: 5 months and 12 days 2020; preservation address: the institute of microorganisms of national academy of sciences of China, no.1, no. 3, north Chen West Lu, the Korean region of Beijing.
The microalgae culture of the invention adopts BG11 culture medium, and the formula is shown in Table 1 and Table 2.
TABLE 1 BG11 Medium
* Table 2 composition of A5+Co solution in Table 1
BG11 liquid media were first prepared according to tables 1 and 2, the pH of the media was adjusted to 8.0, and then microalgae were inoculated into the media, respectively. Culturing in a constant temperature illumination shaking table at 25deg.C for 24 hr with a light-dark time ratio of 14:10 and illumination intensity of 5000Lux at 120rpm to logarithmic phase to obtain microalgae seed liquid.
Example 1
(1) Adding a seed solution of the chain belt alga HCY-BY1 and a microalgae culture medium into a 20L open type photobioreactor, wherein the adding amount of the seed solution is 800mL, the adding amount of the microalgae culture medium is 8L, carrying out alternate light and dark culture, the light and dark period is 24h, the light and dark time ratio is 14:10, the illumination intensity is 5000Lux, the culture temperature is 20 ℃, and the light reaction is carried out BY introducing CO into gas 2 The volume content is 10%, CO in the gas is introduced in the dark reaction 2 The volume content is 10% and the volume content of NO is 0.05%.
(2) After alternate culturing for 3 days, the gas is introduced into the dark reaction to react with light, and NO NO is contained X Reducing the illumination intensity to 3000Lux on the basis of original illumination, culturing for 5 days, entering into growth stabilization period, ending culturing, and detecting the number of mixed bacteria below 2.0X10 3 And each mL.
Microalgae cells are obtained by centrifugation, and the dry weight and the grease content of the cells are measured. And (3) vacuum freeze-drying at-60 ℃ to constant weight, measuring the dry weight of the algae powder, calculating the biomass yield, and measuring the total fat content by adopting an n-hexane-ethyl acetate method. The dry cell weight was found to be 8.3g/L and the oil content was found to be 50.46% of the dry cell weight.
Example 2
The difference from example 1 is that: the adding amount of the seed solution is 800mL, the adding amount of the microalgae culture medium is 4L, the light-dark time ratio is 10:14, the illumination intensity is 10000Lux, the dark reaction is switched to be introduced into gas to react with light after the alternate culture of light and dark for 2 days, and NO NO is contained X The illumination intensity is reduced by 50% on the basis of the original illumination. After the culture is finished, the detection shows that the number of the mixed bacteria is lower than 2.2x10 3 The dry weight of the cells can reach 8.1g/L, and the grease content is 50.12% of the dry weight of the cells.
Example 3
The difference from example 1 is that: the adding amount of the seed solution is 400mL, the adding amount of the microalgae culture medium is 8L, the light-dark time ratio is 12:12, the illumination intensity is 8000Lux, the dark reaction is switched to be introduced into gas to react with light after 5 days of light-dark alternate culture, and NO NO is contained X The illumination intensity is reduced by 20 percent on the basis of the original illumination. After the culture is finished, the detection shows that the number of the mixed bacteria is lower than 2.5X10 3 The dry weight of the cells can reach 8.2g/L, and the grease content is 49.72% of the dry weight of the cells.
Example 4
The difference from example 1 is that: CO 2 The volume content was 5% and the volume content of NO was 0.07%. After the culture is finished, the detection shows that the number of the mixed bacteria is lower than 1.7X10 3 The dry weight of the cells can reach 8.6g/L, and the grease content is 51.93% of the dry weight of the cells.
Example 5
The difference from example 1 is that: CO 2 The volume content was 25% and the volume content of NO was 0.03%. After the culture is finished, the detection shows that the number of the mixed bacteria is lower than 2.1X10 3 The dry weight of the cells can reach 8.4g/L, and the grease content is 50.89% of the dry weight of the cells.
Example 6
The difference from example 1 is that: the microalgae adopts chlorella SF-B1. After the culture is finished, the detection shows that the number of the mixed bacteria is lower than 2.9x10 3 The dry weight of the cells can reach 11.3g/L, and the grease content is 49.41% of the dry weight of the cells.
Example 7
The difference from example 1 is that: the microalgae adopts fibrous algae SS-B7. After the culture is finished, the detection shows that the number of the mixed bacteria is lower than 2.2x10 3 The dry weight of the cells can reach 10.9g/L, and the grease content is 43.89% of the dry weight of the cells.
Comparative example 1
The difference from example 1 is that: the photoreaction and the dark reaction are conducted to the same mixture containing CO only 2 Is free of NO added in dark reaction X . After the culture is finished, the number of the mixed bacteria is 2.1X10 by detection 5 The dry weight of the cells can reach 6.3g/L, and the grease content is 40.23% of the dry weight of the cells.
Comparative example 2
The difference from example 1 is that: the same light reaction and dark reaction are carried out while containing CO 2 And NO X CO in the gas of (2) 2 And NO X The volume content is the same as in example 1. After the culture is finished, the number of the mixed bacteria is 1.6X10 by detection 3 The dry weight of the cells can reach 7.2g/L, and the grease content is 42.36% of the dry weight of the cells.
Comparative example 3
The difference from example 1 is that: after the alternate culture for a period of time, the illumination intensity is not reduced, and the illumination intensity is maintained to be 5000Lux. After the culture is finished, the number of the mixed bacteria is 4.5X10 by detection 3 The dry weight of the cells can reach 8.4g/L, and the grease content is 43.61% of the dry weight of the cells.
Comparative example 4
The difference from example 1 is that: the illumination intensity is reduced after the alternate culture for a period of time, and the NO-containing substances are continuously introduced in the dark reaction X And (5) gas is used until the culture is finished. After the culture is finished, the number of the mixed bacteria is 1.8X10 by detection 3 The dry weight of the cells can reach 7.8g/L, and the grease content is 41.33% of the dry weight of the cells.
Claims (8)
1. Open typeThe method for culturing the oleaginous microalgae is characterized by comprising the following steps: adding microalgae seed liquid and microalgae culture medium into an open type photobioreactor, performing alternate light and dark culture, and introducing CO during photoreaction 2 Introducing CO during dark reaction 2 And NO X The illumination intensity is reduced after the gas is alternately cultured for a period of time, and the NO is stopped being introduced in the dark reaction X Culturing until the culture period is stable, and harvesting microalgae cells;
the microalgae is fibrous algae SS-B7%Ankistrodesmus sp.) SF-B1 of chlorellaChlorella sp.) HCY-BY1 of chain belt algaDesmodesmus abundans) At least one of which has the preservation numbers of CGMCC No. 7478, CGMCC No.11005 and CGMCC No. 19982 respectively;
the light and dark alternate culture is carried out, the culture temperature is 15-35 ℃, the illumination intensity is 2000-20000 Lux, the light and dark period is 24 hours, and the light and dark time ratio is 14:10-10:14; CO-containing photo-reactive feed 2 In the gas, CO 2 The volume content is 5-40%, NO NO is contained X The method comprises the steps of carrying out a first treatment on the surface of the Dark reaction introducing CO 2 And NO X In the gas, CO 2 The volume content is 5-40%, NO X The volume content is 0.03-0.07%; after alternate culturing for 2-5 days, stopping introducing NO X The illumination intensity is reduced by 20 to 50 percent on the basis of the original illumination, and the cultivation is finished.
2. The method according to claim 1, characterized in that: the microalgae is chain belt alga HCY-BY 1%Desmodesmus abundans）。
3. The method according to claim 1, characterized in that: the microalgae culture medium adopts any one of BG11 culture medium, SE culture medium and BBM culture medium.
4. A method according to claim 1, 2 or 3, characterized in that: the preparation method of the microalgae seed liquid comprises the following steps: inoculating microalgae into a microalgae culture medium, and culturing in an oscillating manner until the growth phase of the microalgae seed liquid is logarithmic under the conditions that the pH value is 7-9, the temperature is 20-35 ℃, the illumination period is 24 hours, the light-dark time ratio is 14:10 and the illumination intensity is 2000-20000 Lux, thereby obtaining the microalgae seed liquid.
5. A method according to claim 1 or 3, characterized in that: the volume ratio of the microalgae seed liquid to the microalgae culture medium added into the photobioreactor is 1:20-1:5.
6. The method according to claim 4, wherein: the volume ratio of the microalgae seed liquid to the microalgae culture medium added into the photobioreactor is 1:20-1:5.
7. The method according to claim 1, characterized in that: CO-containing photo-reactive feed 2 In the gas, CO 2 The volume content is 5-20%.
8. The method according to claim 1, characterized in that: said NO X Is NO and/or NO 2 Said CO 2 And NO X The gas is self-made or is flue gas or waste gas, and the flue gas or waste gas is derived from at least one of incineration tail gas and catalytic cracking regeneration tail gas of the sulfur recovery device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011169520.7A CN114507603B (en) | 2020-10-28 | 2020-10-28 | Method for open culture of oleaginous microalgae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011169520.7A CN114507603B (en) | 2020-10-28 | 2020-10-28 | Method for open culture of oleaginous microalgae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114507603A CN114507603A (en) | 2022-05-17 |
| CN114507603B true CN114507603B (en) | 2023-07-04 |
Family
ID=81547092
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011169520.7A Active CN114507603B (en) | 2020-10-28 | 2020-10-28 | Method for open culture of oleaginous microalgae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114507603B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109880856A (en) * | 2017-12-06 | 2019-06-14 | 中国石油化工股份有限公司 | A kind of method of open production microalgae grease |
| CN111100885A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Method for improving oil production of microalgae |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101058535B1 (en) * | 2008-05-22 | 2011-08-23 | 인하대학교 산학협력단 | Microalgae with High Efficient Carbon Dioxide Removal and Its Uses |
| US9074191B2 (en) * | 2009-12-30 | 2015-07-07 | Marcelo Gonzalez Machin | Methods and systems for producing lipids from microalgae using cultured multi-species microalgae |
| CA3017799A1 (en) * | 2016-03-19 | 2017-09-28 | Kiverdi, Inc. | Microorganisms and artificial ecosystems for the production of protein, food, and useful co-products from c1 substrates |
-
2020
- 2020-10-28 CN CN202011169520.7A patent/CN114507603B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109880856A (en) * | 2017-12-06 | 2019-06-14 | 中国石油化工股份有限公司 | A kind of method of open production microalgae grease |
| CN111100885A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Method for improving oil production of microalgae |
Non-Patent Citations (2)
| Title |
|---|
| 影响微藻规模化培养的因素;朱俊英;荣峻峰;宗保宁;;催化学报(第01期);全文 * |
| 微藻固碳技术基础及其生物质应用研究进展;廖莎;薛冬;李晓姝;唐开宇;师文静;李澜鹏;彭绍忠;;当代化工(第06期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114507603A (en) | 2022-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109576315B (en) | Method for producing microalgae grease by using flue gas | |
| Olivieri et al. | Biodiesel production from Stichococcus strains at laboratory scale | |
| CN111100885B (en) | Method for improving oil production of microalgae | |
| CN107460216B (en) | Method for producing microalgae grease by using flue gas | |
| CN111100883B (en) | Method for producing microalgae grease by using flue gas | |
| CN105713950A (en) | Method for producing microalgal oil by using flue gas | |
| CN107287125B (en) | Method for culturing chlorella pyrenoidosa | |
| CN109576314B (en) | Method for preparing microalgae grease through mixed culture | |
| CN111100796B (en) | Scenedesmus rich in oil and culture application thereof | |
| CN109536484B (en) | Method for improving biomass and metabolite of chrysophyceae by magnetic field intervention | |
| CN107460215B (en) | Method for producing grease by microalgae mixed culture | |
| CN109880856B (en) | Open type microalgae grease production method | |
| CN109112073B (en) | Seaweed fermented algae cake | |
| CN116064239A (en) | Grease-rich micro-mango algae and culture application thereof | |
| CN107460217B (en) | Method for preparing microalgae grease through mixed culture | |
| CN105713934A (en) | Method for producing microalgae oil | |
| CN114507603B (en) | Method for open culture of oleaginous microalgae | |
| CN105713951B (en) | Method for preparing microalgae grease | |
| CN114480131B (en) | Open culture method of oleaginous microalgae | |
| CN114426928B (en) | Microalgae culture method for inhibiting mixed bacteria | |
| CN113122455B (en) | Open type culture and microalgae grease production method | |
| CN105713935A (en) | Method for producing lipid through mixed culture of microalgae | |
| CN112725388B (en) | Method for producing microalgae grease by using flue gas | |
| CN116064238A (en) | Method for open culture of oleaginous microalgae | |
| CN117925407A (en) | Open culture method of oleaginous microalgae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231207 Address after: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec (Dalian) Petrochemical Research Institute Co.,Ltd. Address before: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: DALIAN RESEARCH INSTITUTE OF PETROLEUM AND PETROCHEMICALS, SINOPEC Corp. |
|
| TR01 | Transfer of patent right |