CN112725388B - Method for producing microalgae grease by using flue gas - Google Patents
Method for producing microalgae grease by using flue gas Download PDFInfo
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- CN112725388B CN112725388B CN201911033004.9A CN201911033004A CN112725388B CN 112725388 B CN112725388 B CN 112725388B CN 201911033004 A CN201911033004 A CN 201911033004A CN 112725388 B CN112725388 B CN 112725388B
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- 239000003546 flue gas Substances 0.000 title claims abstract description 21
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 8
- 239000004519 grease Substances 0.000 title claims abstract description 7
- 235000007122 Scenedesmus obliquus Nutrition 0.000 claims abstract description 34
- 241000195663 Scenedesmus Species 0.000 claims abstract description 33
- 239000007788 liquid Substances 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 28
- 239000001963 growth medium Substances 0.000 claims abstract description 26
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 22
- 238000012258 culturing Methods 0.000 claims abstract description 15
- 239000000779 smoke Substances 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract 2
- 238000005286 illumination Methods 0.000 claims description 38
- 239000007789 gas Substances 0.000 claims description 14
- 238000004321 preservation Methods 0.000 claims description 13
- 240000009108 Chlorella vulgaris Species 0.000 claims description 5
- 235000007089 Chlorella vulgaris Nutrition 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- 230000008929 regeneration Effects 0.000 claims description 4
- 238000011069 regeneration method Methods 0.000 claims description 4
- 241000195651 Chlorella sp. Species 0.000 claims description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 2
- 238000004523 catalytic cracking Methods 0.000 claims description 2
- 238000009630 liquid culture Methods 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims description 2
- 230000006641 stabilisation Effects 0.000 claims description 2
- 238000011105 stabilization Methods 0.000 claims description 2
- 229910052717 sulfur Inorganic materials 0.000 claims description 2
- 239000011593 sulfur Substances 0.000 claims description 2
- 244000249201 Scenedesmus obliquus Species 0.000 claims 6
- 241000195662 Tetradesmus obliquus Species 0.000 abstract description 29
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052799 carbon Inorganic materials 0.000 abstract description 4
- 239000003054 catalyst Substances 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 46
- 229910002092 carbon dioxide Inorganic materials 0.000 description 29
- 241000195493 Cryptophyta Species 0.000 description 17
- 239000002028 Biomass Substances 0.000 description 14
- 238000001514 detection method Methods 0.000 description 13
- OAYLNYINCPYISS-UHFFFAOYSA-N ethyl acetate;hexane Chemical compound CCCCCC.CCOC(C)=O OAYLNYINCPYISS-UHFFFAOYSA-N 0.000 description 7
- 150000002632 lipids Chemical class 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 238000009777 vacuum freeze-drying Methods 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 238000009629 microbiological culture Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 239000003225 biodiesel Substances 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000000446 fuel Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000009919 sequestration Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000693844 Chlamydatus obliquus Species 0.000 description 1
- 241000893951 Monostroma Species 0.000 description 1
- 241001497549 Scenedesmus acutus Species 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 235000019387 fatty acid methyl ester Nutrition 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000002780 melanosome Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 229930010796 primary metabolite Natural products 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/50—Sulfur oxides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/56—Nitrogen oxides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2258/00—Sources of waste gases
- B01D2258/02—Other waste gases
- B01D2258/0283—Flue gases
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
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- Y02P20/59—Biological synthesis; Biological purification
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Abstract
The invention relates to a method for producing microalgae grease by using smoke, which is characterized in that a microalgae culture medium, scenedesmus HY-D3 seed liquid and chlorella SF-B1 seed liquid are added into a photobioreactor, and CO is introduced25-45% of volume content and SO2Smoke with volume content not more than 0.06% and NOx not more than 0.08%, culturing for 12-48 hr, adding Scenedesmus obliquus FSH-Y2 seed liquid, and mixing. The method improves the microalgae culture system to high-concentration CO2The tolerance and the solubility of the catalyst improve the carbon fixation efficiency, and simultaneously can remove SOx and NOx in the flue gas and purify the flue gas.
Description
Technical Field
The invention belongs to the field of biotechnology and biological energy, and particularly relates to a method for producing microalgae grease by using flue gas.
Background
As fossil energy is decreasing and the greenhouse effect is increasing due to the use of fossil energy, more and more researchers are focusing on the development and utilization of renewable energy. Biomass can be the most important renewable energy on earth, and comprises forestry biomass, crops, aquatic plants, agricultural wastes and the like. Among the many biomass energy sources, microalgae are important renewable resources. They have the characteristics of wide distribution, large biomass, high photosynthesis efficiency, strong environment adaptability, short growth period, high biomass yield and the like. The cells contain unique primary or secondary metabolites and are chemically complex. The solar energy conversion efficiency of the microalgae can reach 3.5 percent, the microalgae is a potential resource for producing medicines, fine chemicals and novel fuels, and fatty acid obtained from the microalgae can be converted into fatty acid methyl ester, namely biodiesel. Therefore, biodiesel produced using microalgal oil as a feedstock is currently the most likely renewable energy source for fuels needed for world transportation.
With the development of the world economy, the use and consumption of a large amount of fossil energy, resulting in the shortage of energy and the increasing deterioration of the environment, particularly CO2The greenhouse effect is getting more and more serious due to the sharp increase of the amount of the organic compound. Short growth period of microalgae, high photosynthetic efficiency, and CO2High fixing efficiency which is more than 10 times of that of terrestrial plants under certain conditions, and can reduce CO2Discharge and simultaneously reduce the culture cost. CO removal2Besides, some microalgae have tolerance and removal capacity of SOx and NOx, and SOx and NOx in the exhaust gas can be purified along with metabolism of the microalgae, so that the emission of harmful gases is effectively reduced.
CN106467897A discloses a Scenedesmus (Scenedesmus) rich in oilDesmodesmus sp.) MH-04 with the preservation number of CGMCC number 10764. The Scenedesmus can tolerate high concentration of CO2And SO2Can use CO-containing2And SO2The waste gas or the flue gas is subjected to illumination autotrophic growth to obtain biomass, and the carbon sequestration efficiency is high. However, this strain is SO-deprived2Has limited capabilities.
CN109576315A discloses a method for producing microalgae oil by using smoke, firstly adding microalgae culture medium and quasi-chlorella cathayi FSH-Y3 or/and Scenedesmus obliquus FSH-Y2 seed liquid into a photobioreactorAdjusting the pH value to 10-12, and introducing CO2Culturing the smoke with the content of 1-5 v% for a certain time; then adjusting the pH value to 8-10, inoculating a chlorella SF-B1 seed solution, simultaneously inoculating at least one of Scenedesmus MH-04 seed solution and Monostroma SS-B1 seed solution, performing mixed culture, and introducing CO2And (3) culturing the smoke with the content of 5-45 v% to a stable period under the condition of continuous illumination, and harvesting the microalgae cells. The method improves the tolerance to SOx and NOx in the flue gas, can purify the flue gas, and has the NOx removal rate of over 80 percent but limited SOx removal effect.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for producing microalgae grease by using flue gas. The method improves the microalgae culture system to high-concentration CO2The tolerance and the solubility of the catalyst improve the carbon fixation efficiency, and simultaneously can remove SOx and NOx in the flue gas and purify the flue gas.
The method for producing the microalgae grease by using the flue gas comprises the following steps:
adding microalgae culture medium and Scenedesmus obliquus (Scenedesmus obliquus) (A) into a photobioreactorScenedesmus acutus) HY-D3 seed liquid, and Chlorella (Chlorella)Chlorella sp.) SF-B1 seed liquid, and introducing CO25-45% of volume content and SO2Flue gas with volume content not more than 0.06% and NOx volume content not more than 0.08%, culturing for 12-48 hr, adding Scenedesmus obliquus (Scenedesmus obliquus) (A. obliquus)Scenedesmus obliqnus) And performing mixed culture on the FSH-Y2 seed liquid.
Wherein Scenedesmus (A) and (B)Scenedesmus acutus) The accession number of HY-D3 is CGMCC number 15298; chlorella (Chlorella vulgaris)Chlorella sp.) The preservation number of SF-B1 is CGMCC No. 11005; scenedesmus obliquus (C. obliquus)Scenedesmus obliqnus) The preservation number of FSH-Y2 is CGMCC number 6551.
In the present invention, Scenedesmus (A) isScenedesmus acutus) HY-D3 has been deposited in China general microbiological culture Collection center (CGMCC) No. 15298 at 2018, 2.5.4 and deposited at Beijing Kogyang area, Xilu No.1 Hospital No. 3 of China academy of sciencesThe location was investigated.
In the present invention, the chlorella isChlorella sp.) SF-B1, which has been deposited in the general microbiological center of China Committee for culture Collection of microorganisms (CGMCC) No.11005 at 7/6/2015 with the deposition address of the institute for microbiology, China academy of sciences No. 3, North West Lu No.1 Hospital, Chaoyang, Beijing. Chlorella SF-B1 was filed as published in CN109576158A and submitted for deposit and proof of survival.
In the present invention, the microalgae culture medium is a liquid culture medium for culturing microalgae, such as BG11, SE, BBM, etc., which are well known to those skilled in the art.
The preparation method of the Scenedesmus HY-D3 seed liquid and the chlorella SF-B1 seed liquid comprises the following steps: adjusting the pH value of the culture medium to 7-9, performing shaking culture at the temperature of 20-30 ℃, the illumination period of 24h, the light-dark time ratio of 14:10 and the illumination intensity of 2000-10000 Lux until the logarithmic phase.
In the invention, the volume ratio of the scenedesmus HY-D3 seed solution to the microalgae culture medium added into the photobioreactor is 1: 20-1: 5.
In the invention, the volume ratio of the chlorella SF-B1 seed solution to the microalgae culture medium added into the photobioreactor is 1: 20-1: 5.
In the invention, the flue gas is derived from incineration tail gas, catalytic cracking regeneration tail gas or S-zorb regeneration tail gas of a sulfur recovery device, preferably, CO in the flue gas2The volume content is 10-30 percent, and SO2The volume content is 0.005-0.06%, and the volume content of NOx is 0.005-0.08%.
In the present invention, Scenedesmus obliquus (A), (B), (C) and (C)Scenedesmus obliqnus) FSH-Y2, which has been deposited in China general microbiological culture Collection center (CGMCC) at 11.9.2012, with the preservation number of CGMCC number 6551 and the preservation address of China academy of sciences (Ministry of sciences, China, No. 3, of Xilu 1, Beijing, Chaoyang, and the area of Tokyo. FSH-Y2 was filed as published in CN104611227A and submitted for deposit and proof of survival.
In the invention, the preparation method of the Scenedesmus obliquus FSH-Y2 seed liquid comprises the following steps: adjusting the pH value of the culture medium to 10-12, and carrying out shake culture to logarithmic phase under the conditions that the temperature is 20-30 ℃, the illumination period is 24 hours, the light-dark time ratio is 14:10, and the illumination intensity is 2000-10000 Lux.
In the invention, the volume ratio of the addition amount of the Scenedesmus obliquus FSH-Y2 seed liquid to the microalgae culture medium is 1: 40-1: 20. Preferably, the scenedesmus obliquus FSH-Y2 seed liquid is added to the photobioreactor in batches every 24-72 hours in the culture process, and the adding amount of each time is an average value according to the total adding amount.
In the invention, the temperature of a microalgae culture system is controlled to be 20-35 ℃, the illumination period is 24h, the light-dark time ratio is 14:10, the illumination intensity is 2000-20000 Lux, and the microalgae culture system is cultured until the growth stabilization period is finished. Preferably, the culture process is divided into two stages by taking Scenedesmus obliquus FSH-Y2 as a node, wherein the illumination intensity of the first stage is 2000-6000 Lux, the illumination period is 24h, and the light-dark time ratio is 14: 10; and in the second stage, the illumination intensity is improved by 3000-10000 Lux, and the illumination is continued until the culture is finished, so that the oil content is improved.
In the invention, microalgae cells are harvested by centrifugation, sedimentation and other modes, the dry weight of the cells and the oil content are measured, the dry weight of the cells can reach more than 11g/L, and the oil content can reach more than 46 percent of the dry weight of the cells.
Compared with the prior art, the invention can bring the following beneficial effects:
(1) in the invention, Scenedesmus HY-D3 and chlorella SF-B1 are added simultaneously in the process of producing microalgae oil by using smoke, and the two algae are matched with each other, so that the inhibiting factors in the smoke can be eliminated simultaneously, the carbon sequestration efficiency is higher, and the obtained biomass contains more oil.
(2) In the culture process of Scenedesmus HY-D3, the pH value is in a rising trend along with the culture, the tolerance capacity of Scenedesmus HY-D3 is unstable, Scenedesmus obliquus FSH-Y2 seed liquid is supplemented at certain time intervals under the condition, the improvement of the tolerance of Scenedesmus obliquus HY-D3 is facilitated, oil in algae cells can be stably accumulated, and finally the oil yield is improved.
(3) The mixed culture system can efficiently purify the flue gas, and the carbon dioxide removal rate in the purified flue gas is 50 percentAbove, SO2The removal rate is more than 90%, and the NOx removal rate is more than 80%.
Detailed Description
The present invention will be described in further detail by way of examples. The embodiments are implemented on the premise of the technical scheme of the invention, and detailed implementation modes and specific operation processes are given, but the protection scope of the invention is not limited by the following embodiments.
The experimental procedures in the following examples are, unless otherwise specified, conventional in the art. The experimental materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified.
In the present invention, CO is contained in the gas2、SO2、NOXThe concentration is detected by a flue gas analyzer.
Gas removal rate = (gas concentration at reactor inlet-gas concentration at reactor outlet)/gas concentration at reactor inlet × 100%.
The scenedesmus HY-D3 is a new strain separated and screened by the inventor and is preserved in the China general microbiological culture Collection center; address: the institute of microbiology, national academy of sciences No. 3, Xilu No.1, Beijing, Chaoyang, Beijing; the preservation number is: CGMCC number 15298; the preservation date is as follows: year 2018, month 2 and day 5. Scenedesmus HY-D3 can tolerate high concentration of SO2Can utilize CO in the exhaust gas2Autotrophic growth and CO fixation2And the problem of greenhouse effect brought by the current industrial society is solved. Under a microscope, algae cells are green and are often aggregated into groups, the groups are usually composed of 4-8 cells, and the cells of the groups are arranged in a straight line. The single cells are oval and oval, the single cells are oval, the cell walls are smooth, the single cells are internally provided with melanosomes, each cell contains a protein nucleus, and the length of each single cell is about 3-6 mu m, and the width of each single cell is about 2-3 mu m. The upper and lower ends of the cells on both sides of the population have 1 long or straight or slightly curved spikes, and both ends of the cells in the middle and the side free parts of the cells on both sides have no spikes. 4 cells with a group width of 10-24 microns and a puncture length of 10-13 microns.
BG11 medium was used in the examples of the present invention, and the medium formulations are shown in tables 1 and 2.
TABLE 1 BG11 culture Medium
Table 2 composition of a5+ Co solution in table 1
BG11 liquid medium was prepared according to tables 1 and 2, the pH of the medium for culturing Scenedesmus obliquus FSH-Y2 was adjusted to 10, the pH of the medium for culturing Scenedesmus obliquus HY-D3 and Chlorella vulgaris SF-B1 was adjusted to 8.0, and then Scenedesmus obliquus FSH-Y2, Scenedesmus obliquus HY-D3 and Chlorella vulgaris SF-B1 were inoculated into the above medium, respectively. Culturing in constant temperature light shaking table at 25 deg.C for 24 hr with light-dark time ratio of 14:10 and light intensity of 5000Lux at 120rpm until logarithmic phase to obtain Scenedesmus obliquus FSH-Y2 seed solution, Scenedesmus obliquus HY-D3 seed solution and Chlorella vulgaris SF-B1 seed solution, and storing the seed solutions at 15 deg.C under weak light.
Example 1
Adding 8L BG11 microalgae culture medium, 400mL Scenedesmus HY-D3 seed solution and 400mL Chlorella SF-B1 seed solution into 20L photobioreactor, and introducing CO240 volume percent of NO, 0.08 volume percent of SO2Smoke with the volume content of 0.06 percent is added into 300mL of Scenedesmus obliquus FSH-Y2 seed liquid once after being cultured for 24 hours. The culture temperature of the culture system is controlled at 25 ℃, the illumination period is 24h, the light-dark time ratio is 14:10, and the illumination intensity is 10000 Lux. And (3) finishing the culture after 7 days, centrifuging to collect algae liquid, measuring the dry weight of algae powder after vacuum freeze drying is carried out at the temperature of-60 ℃ to constant weight, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. After detection, the dry cell weight can reach 12.3g/L, and the oil content is 46.5% of the dry cell weight. Detected and calculated, CO2The removal rate is 51.5 percent, and SO2The removal rate was 92.5%, and the NO removal rate was 82.8%.
Example 2
Adding 8L BG11 microalgae culture medium, 800mL Scenedesmus HY-D3 seed solution and 800mL Chlorella SF-B1 seed solution into 20L photobioreactor, and introducing CO240 volume percent of NO, 0.08 volume percent of SO2Smoke with the volume content of 0.06 percent is added into 200mL of Scenedesmus obliquus FSH-Y2 seed liquid once after culturing for 12 h. The culture temperature of the culture system is controlled to be 35 ℃, the illumination period is 24h, the light-dark time ratio is 14:10, and the illumination intensity is 10000 Lux. And (3) finishing the culture after 7 days, centrifuging to collect algae liquid, measuring the dry weight of algae powder after vacuum freeze drying is carried out at the temperature of-60 ℃ to constant weight, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. After detection, the dry cell weight can reach 12.5g/L, and the oil content is 47.5 percent of the dry cell weight. Detected and calculated, CO2The removal rate is 52.3 percent, and SO2The removal rate was 93.2% and the NO removal rate was 83.1%.
Example 3
Adding 8L BG11 microalgae culture medium, 600mL Scenedesmus HY-D3 seed solution and 200mL Chlorella SF-B1 seed solution into 20L photobioreactor, and introducing CO240 volume percent of NO, 0.08 volume percent of SO2Smoke with the volume content of 0.06 percent is added into 400mL of Scenedesmus obliquus FSH-Y2 seed liquid once after culturing for 48 hours. The culture temperature of the culture system is controlled to be 20 ℃, the illumination period is 24h, the light-dark time ratio is 14:10, and the illumination intensity is 10000 Lux. And (3) finishing the culture after 7 days, centrifuging to collect algae liquid, measuring the dry weight of algae powder after vacuum freeze drying is carried out at the temperature of-60 ℃ to constant weight, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. After detection, the dry cell weight can reach 12.1g/L, and the oil content is 46.2% of the dry cell weight. Detected and calculated, CO2The removal rate is 51.1 percent, and SO2The removal rate was 91.8%, and the NO removal rate was 81.7%.
Example 4
Adding 8L BG11 microalgae culture medium, 400mL Scenedesmus HY-D3 seed solution and 400mL Chlorella SF-B1 seed solution into 20L photobioreactor, and introducing CO240 volume percent of NO, 0.08 volume percent of SO2The volume content is 0.06% smoke, 50mL of Scenedesmus obliquus FSH-Y2 seed solution was added every 24h, for a total of 300 mL. The culture temperature of the culture system is controlled at 25 ℃, the illumination period is 24h, the light-dark time ratio is 14:10, and the illumination intensity is 10000 Lux. And (3) finishing the culture after 7 days, centrifuging to collect algae liquid, measuring the dry weight of algae powder after vacuum freeze drying is carried out at the temperature of-60 ℃ to constant weight, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. After detection, the dry cell weight can reach 13.3g/L, and the oil content is 48.1 percent of the dry cell weight. Detected and calculated, CO2The removal rate is 54.1 percent, and SO2The removal rate was 93.3% and the NO removal rate was 84.6%.
Example 5
Adding 8L BG11 microalgae culture medium, 400mL Scenedesmus HY-D3 seed solution and 400mL Chlorella SF-B1 seed solution into 20L photobioreactor, and introducing CO240 volume percent of NO, 0.08 volume percent of SO2Smoke with volume content of 0.06 percent is added with 100mL of scenedesmus obliquus FSH-Y2 seed liquid every 48 hours, and 300mL is added totally. The culture temperature of the culture system is controlled at 25 ℃, the illumination period is 24h, the light-dark time ratio is 14:10, and the illumination intensity is 10000 Lux. And (3) finishing the culture after 7 days, centrifuging to collect algae liquid, measuring the dry weight of algae powder after vacuum freeze drying is carried out at the temperature of-60 ℃ to constant weight, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. After detection, the dry cell weight can reach 13.2g/L, and the oil content is 47.9 percent of the dry cell weight. Detected and calculated, CO2The removal rate is 53.9 percent, SO2The removal rate was 93.1%, and the NO removal rate was 84.0%.
Example 6
Adding 8L BG11 microalgae culture medium, 400mL Scenedesmus HY-D3 seed solution and 400mL Chlorella SF-B1 seed solution into 20L photobioreactor, and introducing CO230 volume percent of NO, 0.04 volume percent of SO2Smoke with the volume content of 0.03 percent is added into 300mL of Scenedesmus obliquus FSH-Y2 seed liquid once after being cultured for 24 hours. The culture temperature of the culture system is controlled at 25 ℃, the illumination period is 24h, the light-dark time ratio is 14:10, and the illumination intensity is 10000 Lux. Culturing for 7 days, centrifuging to collect algae liquid, and culturing at-60 deg.CAnd (4) measuring the dry weight of the algae powder after vacuum freeze drying to constant weight, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. After detection, the dry cell weight can reach 13.1g/L, and the oil content is 47.5 percent of the dry cell weight. Detected and calculated, CO2The removal rate is 65.1 percent, and SO2The removal rate was 95.3%, and the removal rate of NO was 88.8%.
Example 7
Adding 8L BG11 microalgae culture medium, 400mL Scenedesmus HY-D3 seed solution and 400mL Chlorella SF-B1 seed solution into 20L photobioreactor, and introducing CO240 volume percent of NO, 0.08 volume percent of SO2Smoke with the volume content of 0.06 percent, the culture temperature of a culture system is controlled to be 25 ℃, the illumination period is 24 hours, the light-dark time ratio is 14:10, and the illumination intensity is 5000 Lux; after 24h of culture, 300mL of Scenedesmus obliquus FSH-Y2 seed liquid is added at one time, the illumination intensity is increased by 10000Lux, and the illumination is continued until the culture is finished. And (3) finishing the culture after 7 days, centrifuging to collect algae liquid, measuring the dry weight of algae powder after vacuum freeze drying is carried out at the temperature of-60 ℃ to constant weight, calculating the biomass yield, and measuring the total lipid content by adopting a normal hexane-ethyl acetate method. After detection, the dry cell weight can reach 12.9g/L, and the oil content is 48.5% of the dry cell weight. Detected and calculated, CO2The removal rate is 51.6 percent, and SO2The removal rate was 92.7%, and the NO removal rate was 83.1%.
Comparative example 1
The difference from example 1 is that: seed liquid without Chlorella SF-B1 added. After 7 days of culture, the dry weight of the cells can reach 10.8g/L after detection, and the oil content is 45.7 percent of the dry weight of the cells. Detected and calculated, CO2The removal rate is 50.6 percent, and SO2The removal rate was 90.5%, and the removal rate of NO was 10.1%.
Comparative example 2
The same as example 1, except that: no Scenedesmus HY-D3 seed solution was added. After 7 days of culture, the dry weight of the cells can reach 10.2g/L after detection, and the oil content is 43.4 percent of the dry weight of the cells. Detected and calculated, CO2The removal rate is 50.7 percent, SO2The removal rate is 50.5%, and the removal rate of NO is 80.1%.
Comparative example 3
The same as example 1, except that: scenedesmus obliquus FSH-Y2 was not added. After 7 days of culture, the dry weight of the cells can reach 11.5g/L after detection, and the oil content is 44.6 percent of the dry weight of the cells. Detected and calculated, CO2The removal rate is 51.7 percent, and SO2The removal rate is 85.5 percent, and the removal rate of NO is 82.1 percent.
Comparative example 4
The difference from example 1 is that: scenedesmus MH-04 with preservation number CGMCC No.10764 as CN106467897A is used to replace Scenedesmus HY-D3. After detection, the dry cell weight can reach 12.0g/L, and the oil content is 45.6% of the dry cell weight. Detected and calculated, CO2The removal rate is 51.7 percent, and SO2The removal rate was 60.5%, and the NO removal rate was 78.7%.
Comparative example 5
The difference from example 1 is that: adopts the unicladium SS-B1 with the preservation number of CGMCC No.7479 described in CN104611228A to replace scenedesmus HY-D3. After detection, the dry cell weight can reach 10.4g/L, and the oil content is 44.6% of the dry cell weight. Detected and calculated, CO2The removal rate is 50.7 percent, and SO2The removal rate was 65.5%, and the NO removal rate was 79.1%.
Comparative example 6
The same as example 1, except that: adopts the fibrophyta SS-B7 described in CN105713836A, the preservation number is CGMCC No.7478 to replace Scenedesmus SF-B1. After detection, the dry cell weight can reach 11.8g/L, and the oil content is 45.7% of the dry cell weight. Detected and calculated, CO2The removal rate is 51.7 percent, and SO2The removal rate is 90.3%, and the removal rate of NO is 20.3%.
Claims (12)
1. A method for producing microalgae grease by using flue gas is characterized by comprising the following steps: adding microalgae culture medium, Scenedesmus HY-D3 seed solution, and Chlorella (Chlorella vulgaris)Chlorella sp.) SF-B1 seed liquid, and introducing CO25-45% of volume content and SO2Culturing smoke with volume content not more than 0.06% and NOx volume content not more than 0.08% for 12-48 hr, adding Scenedesmus obliquus FSH-Y2 seed solution, and mixing; wherein the preservation number of the scenedesmus HY-D3 is CGMCC number 15298; the preservation number of the chlorella is CGMCC No. 11005; the preservation number of the Scenedesmus obliquus FSH-Y2 is CGMCC number 6551.
2. The method of claim 1, wherein: the microalgae culture medium adopts BG11, SE and BBM liquid culture medium for culturing microalgae.
3. The method of claim 1, wherein: the preparation method of the Scenedesmus HY-D3 seed liquid and the chlorella SF-B1 seed liquid comprises the following steps: adjusting the pH value of the culture medium to 7-9, performing shaking culture at the temperature of 20-30 ℃, the illumination period of 24h, the light-dark time ratio of 14:10 and the illumination intensity of 2000-10000 Lux until the logarithmic phase.
4. The method of claim 1, wherein: the volume ratio of the scenedesmus HY-D3 seed solution to the microalgae culture medium added into the photobioreactor is 1: 20-1: 5.
5. The method of claim 1, wherein: the volume ratio of the chlorella SF-B1 seed solution to the microalgae culture medium added into the photobioreactor is 1: 20-1: 5.
6. The method of claim 1, wherein: the flue gas is derived from incineration tail gas of a sulfur recovery device, catalytic cracking regeneration tail gas or S-zorb regeneration tail gas.
7. The method according to claim 1 or 6, characterized in that: CO in flue gas2The volume content is 10-30 percent, and SO2The volume content is 0.005-0.06%, and the volume content of NOx is 0.005-0.08%.
8. The method of claim 1, wherein: the preparation method of the Scenedesmus obliquus FSH-Y2 seed liquid comprises the following steps: adjusting the pH value of the culture medium to 10-12, and carrying out shake culture to logarithmic phase under the conditions that the temperature is 20-30 ℃, the illumination period is 24 hours, the light-dark time ratio is 14:10, and the illumination intensity is 2000-10000 Lux.
9. The method of claim 1, wherein: the volume ratio of the addition amount of the Scenedesmus obliquus FSH-Y2 seed liquid to the microalgae culture medium is 1: 40-1: 20.
10. The method according to claim 1 or 9, characterized in that: and adding the Scenedesmus obliquus FSH-Y2 seed solution into the photobioreactor in batches every 24-72 hours in the culture process, wherein the adding amount of each time is an average value according to the total adding amount.
11. The method of claim 1, wherein: controlling the temperature of a microalgae culture system to be 20-35 ℃, the illumination period to be 24h, the light-dark time ratio to be 14:10, the illumination intensity to be 2000-20000 Lux, and culturing until the growth stabilization period is finished.
12. The method of claim 1, 9 or 11, wherein: the culture process is divided into two stages by taking Scenedesmus obliquus FSH-Y2 as a node, wherein the illumination intensity of the first stage is 2000-6000 Lux, the illumination period is 24h, and the light-dark time ratio is 14: 10; and in the second stage, the illumination intensity is increased by 3000-10000 Lux, and the illumination is continued until the culture is finished.
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