CN107460216A - A kind of method that microalgae grease is produced using flue gas - Google Patents

A kind of method that microalgae grease is produced using flue gas Download PDF

Info

Publication number
CN107460216A
CN107460216A CN201610386730.9A CN201610386730A CN107460216A CN 107460216 A CN107460216 A CN 107460216A CN 201610386730 A CN201610386730 A CN 201610386730A CN 107460216 A CN107460216 A CN 107460216A
Authority
CN
China
Prior art keywords
algae
seed liquors
flue gas
seed
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610386730.9A
Other languages
Chinese (zh)
Other versions
CN107460216B (en
Inventor
师文静
王鹏翔
廖莎
孙启梅
李晓姝
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
China Petroleum and Chemical Corp
Sinopec Fushun Research Institute of Petroleum and Petrochemicals
Original Assignee
China Petroleum and Chemical Corp
Sinopec Fushun Research Institute of Petroleum and Petrochemicals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by China Petroleum and Chemical Corp, Sinopec Fushun Research Institute of Petroleum and Petrochemicals filed Critical China Petroleum and Chemical Corp
Priority to CN201610386730.9A priority Critical patent/CN107460216B/en
Publication of CN107460216A publication Critical patent/CN107460216A/en
Application granted granted Critical
Publication of CN107460216B publication Critical patent/CN107460216B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/50—Carbon oxides
    • B01D2257/504—Carbon dioxide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2258/00—Sources of waste gases
    • B01D2258/02—Other waste gases
    • B01D2258/0283—Flue gases
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/59—Biological synthesis; Biological purification

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of method that microalgae grease is produced using flue gas, including（1）Micro-algae culture medium and kelvin are intended into chlorella FSH Y3 seed liquors to be added in bioreactor, regulation cultivating system PH is 10~12, and is passed through CO2Volume content is 1v%~5v% flue gas, is cultivated 25 days；（2）The pH value for adjusting cultivating system is 8~10, accesses grid algae（Desmodesmus sp.）MH 04, single needle algae（MonorapHidium sp）One or both of SS B1 seed liquors, while incoming fiber algae（Ankistrodesmus sp.）SS B7 seed liquors are mixed, and are passed through CO2Volume content is 5v%~45v% flue gas, is cultivated under the conditions of continuous illumination to stationary phase, harvesting microalgae cell.The inventive method improves microdisk electrode system to high concentration CO2Tolerance and dissolubility, improve carbon sequestration efficiency, while improve the tolerance to SOx, NOx in flue gas, the harvest yield of microalgae grease significantly improves.

Description

A kind of method that microalgae grease is produced using flue gas
Technical field
The invention belongs to biotechnology and field of biological energy source, and in particular to a kind of side that microalgae grease is produced using flue gas Method.
Background technology
Due to the increase for reducing increasingly and greenhouse effects being caused using fossil energy of fossil energy, increasing scientific research Worker focuses on sight in the development and utilization of regenerative resource.Biomass energy is as most important renewable energy on the earth Source, it includes forestry biomass, crops, water plant, agricultural wastes etc..In many biomass energies, microalgae is Important renewable resource.They have widely distributed, biomass is big, photosynthetic efficiency is high, strong environmental adaptability, growth The features such as cycle is short, biomass yield is high.Primary or secondary metabolite containing uniqueness in its cell, complex chemical composition.It is micro- The solar conversion efficiency of algae can reach 3.5%, be the potential resource for producing medicine, fine chemical product and New-type fuel, from microalgae In obtained aliphatic acid can change into Fatty acid methyl ester, i.e. biodiesel.
With the development of World Economics, substantial amounts of fossil energy using and consuming, and causes shortage and the environment of the energy Worsening, particularly CO2Sharply increase caused by greenhouse effects it is increasingly severe.The growth cycle of microalgae is short, photosynthetic effect Rate is high, CO2Fixed efficiency is high, up to more than 10 times of terrestrial plant under certain condition, can not only reduce CO2Discharge, simultaneously Also reduce toxigenic capacity；Except CO2Outside, the composition such as some SOx, NOx in waste gas is cleaned place also with the metabolism of microalgae Reason, noxious gas emission is effectively reduced, therefore the biodiesel produced by the use of microalgae grease as raw material is most possible at present Meet the regenerative resource of fuel needed for world's transport.
At present for the more of the oil-producing microalgaes such as chlorella, grid algae research.CN20110144545.6 discloses one plant of grid algae Algae strain, the growth of the algae strain can utilize synthetic medium or appropriately processed waste water to grow, be characterized in that lipid-producing is higher than Most of at present to divide algae strain, the algae strain application field includes CO2Fixation, the purification of waste water, grease, protein, pigment, shallow lake The production of powder, polysaccharide, nucleic acid.CN20120154470.4 disclose one plant of rich oil marine microalgae it is micro- intend ball algae (Nannochloropsis gaditana ) algae strain and its application, the algae strain can in the environment of pH=4.5 normal growth, its oil Fat content is up to 35%.CN20111019480.X discloses one plant of microalgae algae strain（Mychonases sp.）And its for producing life The application of thing diesel oil, the polyunsaturated fatty acid of high added value, including leukotrienes C18 can be produced using the algae strain:3 and nerve Sour C24:1, it obtains the byproduct of high added value while biodiesel is obtained.CN102703326A discloses a kind of height CO2The microalgae and its selection of tolerance and fixed rate, but the algae strain that is provided of the patent is not directed to the grease of the algae strain Content.Or above-mentioned patent can not efficiently utilize CO2Lipid-producing, otherwise fat content is not high enough in the biomass obtained.Especially It is in actual applications, to work as CO2Concentration be higher than 5% when, the growth of most of microalgaes will be suppressed, and the gas of industrial discharge CO in body2Concentration is generally 10%-20%, and contains the material to the toxic effect of microalgae, such as SOx, NOx etc. simultaneously. Therefore, for the CO in the gas of directly fixed industrial discharge2Microalgae except requiring to CO2High conversion rate, growth rate Hurry up, be resistant to pH scopes it is wide outside, to be also resistant to high CO2The harmful substance such as concentration and tolerance SOx, NOx.
Liu Pinghuai etc.（Organic carbon source grows on single needle frustule, oil and fat accumulation and photosynthetic influence, bioengineering, 2012, 33(18): 224-246）A kind of mode of production using organic carbon source culture single needle algae is described, although culture terminates Biomass has exceeded 10g/L, but this kind of mode is single needle algae Heterotrophic culture mode, and the organic carbons such as glucose are utilized in incubation Cell growth is realized in source, and this training method do not utilize CO2Etc. inorganic carbon source economy, and the addition of organic carbon source, Incubation is also easy to produce microbiological contamination problem, influences the growth of frustule.
The content of the invention
In view of the shortcomings of the prior art, the present invention provides a kind of method that microalgae grease is produced using flue gas.Present invention side Method improves microdisk electrode system to high concentration CO2Tolerance and dissolubility, improve carbon sequestration efficiency, while improve to flue gas Middle SOx, NOx tolerance, the harvest yield of microalgae grease significantly improve.
The method that the present invention produces microalgae grease using flue gas, including following content：
（1）Micro-algae culture medium and kelvin are intended into chlorella FSH-Y3 seed liquors to be added in bioreactor, regulation culture body It is that PH is 10~12, and is passed through CO2Volume content is 1v%~5v% flue gas, is cultivated 2-5 days；
（2）The pH value for adjusting cultivating system is 8~10, accesses grid algae（Desmodesmus sp.）MH-04 seed liquors, single needle algae （MonorapHidium sp）One or both of SS-B1 seed liquors, while incoming fiber algae（Ankistrodesmus sp.）SS-B7 seed liquors are mixed, and are passed through CO2Volume content is 5v%~45v% flue gas, in continuous illumination condition It is lower to cultivate to stationary phase, harvesting microalgae cell；
Wherein described kelvin intends chlorella（Parachlorella kessleri）FSH-Y3, grid algae（Desmodesmus sp.）MH-04, single needle algae（MonorapHidium sp）SS-B1 and algae fibre（Ankistrodesmus sp.）SS-B7, respectively " Chinese microorganism strain preservation conservator is preserved on May 26th, 2014, on April 24th, 2015 and on April 15th, 2013 Meeting common micro-organisms center ", deposit number is respectively CGMCC No. 9238, CGMCC No. 10764, CGMCC No. 7479 With CGMCC No. 7478, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences microbe research Institute.Wherein single needle algae（MonorapHidium sp）SS-B1 is the algae strain disclosed in CN201310537912.8.
In the present invention, described kelvin intend chlorella FSH-Y3 algaes strains under the microscope frustule be it is spherical, oval, it is interior There is the chromatoplast of a Zhousheng, cup-shaped or sheet；Vegetative propagation, each cell can produce 2,4,8 or 16 autospores, into Mother cell ruptures when ripe, spore effusion, is new individual after growing up.The algae strain can preferably absorb two under high pH value Carbonoxide, fast-growth breeding.Described grid algae MH-04 algae strains are a kind of green algate of fresh water, and frustule is in green under the microscope, In groups, individual cells are in avette, and cell membrane is smooth for often aggregation, inside have chromatoplast, each cell contains a pyrenoids, single thin Born of the same parents are about 5-6 μm, wide about 2-3 μm.Described single needle algae SS-B1 algae strains are a kind of green algate of fresh water, and frustule is leaf for length, green Color, a length of 10~20 μm of algae kind is wide 2~4 μm, includes pigment, and it is S-shaped that flat board algae, which falls form, bottle green.Grid algae MH-04 and list Pin algae SS-B1 is resistant to the CO of high concentration2And SO2, can utilize and contain CO2And SO2Waste gas or flue gas carry out illumination from health It is long to obtain rich grease-contained biomass, carbon sequestration efficiency high.Described algae fibre SS-B7 algae strains are a kind of green algate of fresh water, micro- Frustule is green under mirror, crescent or arch, grows thickly, bends, tapering thin from mediad both ends, and distal tip is long about 5-6 μm, Center is wide about 2 μm.The algae strain is resistant to the CO of high concentration2And NOx, it can utilize and contain CO2Carried out with NOx waste gas or flue gas Illumination autophyting growth obtains rich grease-contained biomass, carbon sequestration efficiency high.
In the present invention, micro-algae culture medium cultivates the liquid training of microalgae using BG11, SE, BBM well known in the art etc. Support base.
In the present invention, it is as follows that kelvin intends preparing for chlorella FSH-Y3 seed liquors：The PH of culture medium is adjusted to 10~12, It it is 20~30 DEG C, periodicity of illumination 24h in temperature, brightness time ratio is 14:10, intensity of illumination is 2000~20000Lux bar Under part, shaken cultivation to exponential phase.It is preferred that the scenedesmus obliquus described in CN201310537896.2 is added simultaneously （Scenedesmus obliqnus）FSH-Y2 seed liquors, the algae strain were preserved in " Chinese microorganism strain on 11st in September in 2012 Preservation administration committee common micro-organisms center ", deposit number are CGMCC No. 6551, and preservation address is Chaoyang District, Beijing City No. 3 Institute of Microorganism, Academia Sinica of institute of North Star West Road 1.The same kelvin of preparation method of scenedesmus obliquus FSH-Y2 seed liquors Intend chlorella FSH-Y3, addition is that the volume ratio of seed liquor and culture medium is 1:40~1:10.
In the present invention, grid algae MH-04 seed liquors, the preparation method of single needle algae SS-B1 seed liquors are as follows：By micro-algae culture medium PH be adjusted to 7~9, temperature be 20~30 DEG C, periodicity of illumination 24h, brightness time ratio be 14:10, intensity of illumination is 2000~20000Lux, shaken cultivation to exponential phase.
In the present invention, preparing for algae fibre SS-B7 seed liquors is as follows：The PH of culture medium is adjusted to 7~9, is in temperature 20~30 DEG C, periodicity of illumination 24h, brightness time ratio is 14:10, intensity of illumination is 2000~10000Lux, and shaken cultivation is extremely Exponential phase.
Total inoculum concentration that microalgae seed liquor is controlled in the present invention is the 10%~30% of culture medium cumulative volume, and wherein kelvin is intended small Ball algae FSH-Y3 seed liquors, grid algae MH-04 seed liquors and/or single needle algae SS-B1 seed liquors, algae fibre SS-B7 seed liquor threes Volume ratio be 1:6:6~4:1:1.When being inoculated with grid algae MH-04 seed liquors and single needle algae SS-B1 seed liquors simultaneously, the two Volume ratio is 5:1-1:5.
In the present invention, described flue gas derives from sulfur recovery facility incineration tail gas, catalytic cracked regenerated tail gas or S- Zorb regeneration tail gas, wherein CO2Content is 5v%~45v%, SO2Content is no more than 600 × 10-6（v/v）, NOx content do not surpass Cross 500 × 10-6（v/v）.
In the present invention, the temperature of mixed culture is 20~30 DEG C, periodicity of illumination 24h, and brightness time ratio is 14:10, light It is 2000~10000Lux according to intensity, culture to growth stationary phase terminates.By the mode harvesting microalgae cell such as centrifuging, settling, Measure dry cell weight and fat content, dry cell weight can reach more than 12g/L, and the biomass yield of cell reaches 1.5g/ (L D), fat content can reach more than the 45% of dry cell weight, while the removal efficiency of carbon dioxide brings up to more than 50%, NOx removal Rate can reach 20%.
Compared with prior art, the present invention can bring following beneficial effect：
1st, it is preferred individually to be cultivated using the kelvin plan chlorella FSH-Y3 that can tolerate high PH environment, initial high PH and intermittent light According to the growth that can suppress microdisk electrode miscellaneous bacteria at initial stage and pest and disease damage, microalgae is contributed to be in growth vigor；And high PH is favourable In CO2Dissolving, make CO2Easily absorbed by microalgae, be favorably improved CO2Fixed efficiency.
2nd, PH is reduced after cultivating 2-5 days, adds SO in tolerable flue gas2Continuous illumination culture is carried out with NOx microalgae, is had Help promote the fast-growth of microalgae, improve the growth rate of microalgae.Moreover, this several algae can work in coordination, than single algae Kind culture has higher carbon sequestration efficiency, and carbon dioxide eliminating rate is higher, and the biomass of acquisition contains more fat contents.
3rd, co-culture system of the invention is resistant to the CO of high concentration2、SO2And NOx, the CO in waste gas can be utilized2 Carry out autophyting growth, fixed CO2, alleviate greenhouse effects and exhaust pollution problems that current industrial society brings.
Embodiment
The present invention is described in further detail below by embodiment.In the present invention, wt% is mass fraction, and v% is body Fraction.Wherein described biomass yield for harvest algae silty amount g/ (algae solution volume L* incubation time d), removal efficiency are (be passed through Gas content-discharge Gas content)/it is passed through Gas content.Microdisk electrode of the present invention uses BG11 culture mediums, and culture medium prescription is such as Shown in Tables 1 and 2.
The BG11 culture mediums of table 1
* in the table 1 of table 2 A5+Co solution composition
It is first according to Tables 1 and 2 and prepares BG11 fluid nutrient mediums, culture kelvin is intended into chlorella FSH-Y3 and scenedesmus obliquus FSH- Y2 culture medium PH is adjusted to 10, by cultivate grid algae MH-04, single needle algae SS-B1, algae fibre SS-B7 culture medium PH adjust For 8.0, kelvin is then intended into chlorella FSH-Y3, scenedesmus obliquus FSH-Y2, grid algae MH-04, single needle algae SS-B1 and algae fibre SS-B7 is inoculated in above-mentioned culture medium respectively.Cultivated in constant temperature illumination shaking table, cultivation temperature is 25 DEG C, and periodicity of illumination is 24h, brightness time ratio are 14:10, intensity of illumination 5000Lux, 120rpm shaken cultivation to exponential phase, obtain kelvin and intend Chlorella FSH-Y3 seed liquors, scenedesmus obliquus FSH-Y2 seed liquors, grid algae MH-04 seed liquors, single needle algae SS-B1 seed liquors and fibre Algae SS-B7 seed liquors are tieed up, above-mentioned seed liquor is saved backup under 15 DEG C of dim lights.
Embodiment 1
（1）In 10L bioreactors, add kelvin prepared by embodiment 1 and intend chlorella FSH-Y3 seed liquors and microalgae training Support base, the addition of seed liquor is 400mL, and the pH value of micro-algae culture medium is adjusted to 10, addition 8L, the intensity of illumination of culture For 5000Lux, periodicity of illumination 24h, brightness time ratio is 14:10, it is passed through CO in flue gas2Content be 3v%, NO and NO2Content For 50 × 10-6（v/v）, SO2Content is 60 × 10-6（v/v）.
（2）After culture 4 days, grid algae MH-04 seed liquors 400mL and algae fibre SS-B7 prepared by access embodiment 1 seed Liquid 400mL, the PH of regulation microdisk electrode system is 8, continuous illumination culture, intensity of illumination 5000Lux；It is passed through CO in flue gas2 Content be 30v%, NO and NO2Content is 500 × 10-6（v/v）, SO2Content is 600 × 10-6（v/v）.
（3）Culture enters growth stationary phase after 7 days, terminates culture, and microalgae cell is harvested by centrifugation, and determines dry cell weight and oil Fat content.Algae dried bean noodles weight is measured after vacuum freeze drying to constant weight under the conditions of -60 DEG C, calculates yield of biomass, and using just Hexane:Ethyl acetate method measures total lipid content.Dry cell weight can reach 12.7g/L after after testing, and fat content is dry cell weight 45.7%, CO in incubation2Removal efficiency is 51%, and NOx removal rate is 21.3%.
Embodiment 2
（1）In 10L bioreactors, add kelvin prepared by embodiment 1 and intend bead FSH-Y3 seed liquors and microdisk electrode Base, the addition of seed liquor are 800mL, and the pH value of micro-algae culture medium is adjusted to 12, addition 8L, and the intensity of illumination of culture is 5000Lux, periodicity of illumination 24h, brightness time ratio are 14:10, it is passed through CO in flue gas2Content be 5v%, NO and NO2Content is 80×10-6（v/v）, SO2Content is 100 × 10-6（v/v）.
（2）After culture 2 days, grid algae MH-04 seed liquors 560mL and algae fibre SS-B7 prepared by access embodiment 1 seed Liquid 560mL, the PH of regulation microdisk electrode system is 9, continuous illumination culture, intensity of illumination 5000Lux；It is passed through CO in flue gas2 Content be 30v%, NO and NO2Content is 500 × 10-6（v/v）, SO2Content is 600 × 10-6（v/v）.
（3）Culture enters stationary phase after 8 days, terminate culture, and microalgae cell is harvested by centrifugation, and determines dry cell weight and grease contains Amount.Algae dried bean noodles weight is measured after vacuum freeze drying to constant weight under the conditions of -60 DEG C, calculates yield of biomass, and use n-hexane: Ethyl acetate method measures total lipid content.Dry cell weight can reach 12.5g/L, and fat content is the 46.1% of dry cell weight, is cultivated CO in journey2Removal efficiency is 50.4%, and NOx removal rate is 21.9%.
Embodiment 3
（1）In 10L bioreactors, add kelvin prepared by embodiment 1 and intend bead FSH-Y3 seed liquors and microdisk electrode Base, the addition of seed liquor are 800mL, and the pH value of micro-algae culture medium is adjusted to 12, addition 8L, and the intensity of illumination of culture is 5000Lux, periodicity of illumination 24h, brightness time ratio are 14:10, it is passed through CO in flue gas2Content be 5v%, NO and NO2Content is 80×10-6（v/v）, SO2Content is 100 × 10-6（v/v）.
（2）After culture 2 days, single needle algae SS-B1 seed liquors 560mL and algae fibre SS-B7 prepared by access embodiment a kind Sub- liquid 560mL, the PH of regulation microdisk electrode system is 9, continuous illumination culture, intensity of illumination 5000Lux；It is passed through in flue gas CO2Content be 30v%, NO and NO2Content is 500 × 10-6（v/v）, SO2Content is 600 × 10-6（v/v）.
（3）Culture enters stationary phase after 8 days, terminate culture, and microalgae cell is harvested by centrifugation, and determines dry cell weight and grease contains Amount.Algae dried bean noodles weight is measured after vacuum freeze drying to constant weight under the conditions of -60 DEG C, calculates yield of biomass, and use n-hexane: Ethyl acetate method measures total lipid content.Dry cell weight can reach 12.1g/L, and fat content is the 45.6% of dry cell weight, is cultivated CO in journey2Removal efficiency is 50.2%, and NOx removal rate is 20.8%.
Embodiment 4
（1）In 10 bioreactors, add kelvin prepared by embodiment 1 and intend bead FSH-Y3 seed liquors and microdisk electrode Base, the addition of seed liquor are 800mL, and the pH value of micro-algae culture medium is adjusted to 12, addition 8L, and the intensity of illumination of culture is 5000Lux, periodicity of illumination 24h, brightness time ratio are 14:10, it is passed through CO in flue gas2Content be 5v%, NO and NO2Content is 80×10-6（v/v）, SO2Content is 100 × 10-6（v/v）.
（2）After culture 2 days, the grid algae MH-04 seed liquors 280mL of the access preparation of embodiment 1, single needle algae SS-B1 seed liquors 280mL and algae fibre SS-B7 seed liquor 560mL, the PH of regulation microdisk electrode system is 9, continuous illumination culture, intensity of illumination For 5000Lux；It is passed through CO in flue gas2Content be 30v%, NO and NO2Content is 500 × 10-6（v/v）, SO2Content be 600 × 10-6（v/v）.
（3）Culture enters stationary phase after 8 days, terminate culture, and microalgae cell is harvested by centrifugation, and determines dry cell weight and grease contains Amount.Algae dried bean noodles weight is measured after vacuum freeze drying to constant weight under the conditions of -60 DEG C, calculates yield of biomass, and use n-hexane: Ethyl acetate method measures total lipid content.Dry cell weight can reach 13.2g/L, and fat content is the 46.9% of dry cell weight, is cultivated CO in journey2Removal efficiency is 52.7%, and NOx removal rate is 21.1%.
Embodiment 5
Using incubation and condition of culture same as Example 1, difference is：Step（1）Add kelvin and intend bead Algae FSH-Y3 seed liquor 200mL, add scenedesmus obliquus FSH-Y2 seed liquors 200mL.Dry cell weight can reach 13.1g/L, grease Content is the 47.1% of dry cell weight, CO in incubation2Removal efficiency is 51.9%, and NOx removal rate is 21.9%.
Embodiment 6
Using incubation and condition of culture same as Example 3, difference is：Step（2）Add kelvin and intend bead Algae FSH-Y3 seed liquor 400mL, add scenedesmus obliquus FSH-Y2 seed liquors 400mL.Dry cell weight can reach 12.8g/L, grease Content is the 47.1% of dry cell weight, CO in incubation2Removal efficiency is 50.9%, and NOx removal rate is 22.9%.
Comparative example 1
Using incubation and condition of culture same as Example 1, difference is：FSH-Y3 seed liquors, grid algae MH-04 Seed liquor and algae fibre SS-B7 seed liquor are added in reactor together in culture starting.Microalgae is harvested by centrifugation after terminating in culture Cell, dry cell weight and fat content are determined, dry cell weight can reach 11.7g/L, and fat content is the 44.1% of dry cell weight.
Comparative example 2
Using incubation and condition of culture same as Example 1, difference is：FSH-Y3 seed liquor 800mL are added, After culture 4 days, step（2）Only change condition of culture, be added without other microalgaes.Culture terminates culture after 7 days, and microalgae is harvested by centrifugation Cell, determine dry cell weight and fat content.Dry cell weight can reach 3.9g/L, and fat content is the 36.6% of dry cell weight.
Comparative example 3
Using incubation and condition of culture same as Example 1, difference is：Step（1）Add MH-04 seed liquors 800mL, the pH value of culture medium is always 8.Culture terminates culture after 11 days, is harvested by centrifugation microalgae cell, measure dry cell weight and Fat content.Dry cell weight can reach 6.9g/L, and fat content is the 39.8% of dry cell weight.
Comparative example 4
Using incubation and condition of culture same as Example 1, difference is：Step（1）Add algae fibre SS-B7 Seed liquor 800mL, the pH value of culture medium is always 8.Culture terminates culture after 11 days, and microalgae cell is harvested by centrifugation, and determines cell Dry weight and fat content.Dry cell weight can reach 10.2g/L, and fat content is the 41.9% of dry cell weight.
In summary, chlorella FSH-Y3, grid algae MH-04, single needle algae SS-B1 and fibre are intended relative to single algae kind, kelvin Dimension algae SS-B7 takes two-step method to be mixed, and is favorably improved the tolerance of cultivating system, and can obtain higher life Object amount and fat content.The present invention prepares microalgae grease using flue gas, that is, realizes the production of grease, while can purify useless Gas, economic benefit and obvious environment benefit improve.

Claims (12)

  1. A kind of 1. method that microalgae grease is produced using flue gas, it is characterised in that including following content：（1）By micro-algae culture medium with Kelvin is intended chlorella FSH-Y3 seed liquors and is added in bioreactor, and regulation cultivating system PH is 10~12, and is passed through CO2 Volume content is 1v%~5v% flue gas, is cultivated 2-5 days；（2）The pH value for adjusting cultivating system is 8~10, accesses grid algae （Desmodesmus sp.）MH-04 seed liquors, single needle algae（MonorapHidium sp）One kind in SS-B1 seed liquors or two Kind, while incoming fiber algae（Ankistrodesmus sp.）SS-B7 seed liquors are mixed, and are passed through CO2Volume contains The flue gas for 5v%~45v% is measured, is cultivated under the conditions of continuous illumination to stationary phase, harvesting microalgae cell；Wherein described kelvin Intend chlorella（Parachlorella kessleri）FSH-Y3, grid algae（Desmodesmus sp.）MH-04, single needle algae （MonorapHidium sp）SS-B1 and algae fibre（Ankistrodesmus sp.）SS-B7, respectively on May 26th, 2014, On April 24th, 2015 and on April 15th, 2013 are preserved in " in China Committee for Culture Collection of Microorganisms's common micro-organisms The heart ", deposit number are respectively CGMCC No. 9238, CGMCC No. 10764, CGMCC No. 7479 and CGMCC No. 7478。
  2. 2. in accordance with the method for claim 1, it is characterised in that：Described kelvin intends chlorella FSH-Y3 algae strains in microscope Lower frustule is spherical, oval, inside there is the chromatoplast of a Zhousheng, cup-shaped or sheet；Vegetative propagation, each cell can produce Raw 2,4,8 or 16 autospores, mother cell ruptures when ripe, spore effusion, is new individual after growing up；Described grid algae MH-04 algae strains are a kind of green algate of fresh water, and frustule is in green under the microscope, and in groups, individual cells are in avette, cell for often aggregation Wall is smooth, inside there is a chromatoplast, and each cell contains a pyrenoids, and individual cells are about 5-6 μm, wide about 2-3 μm；Described list Pin algae SS-B1 algae strains are a kind of green algate of fresh water, and frustule is leaf for length, and green, a length of 10~20 μm of algae kind is wide 2~4 μm, interior Containing pigment, it is S-shaped that flat board algae, which falls form, bottle green；Described algae fibre SS-B7 algae strains are a kind of green algate of fresh water, in microscope Lower frustule is green, crescent or arch, grows thickly, bends, tapering thin from mediad both ends, and distal tip is long about 5-6 μm, in Entreat wide about 2 μm.
  3. 3. in accordance with the method for claim 1, it is characterised in that：Micro-algae culture medium is using BG11, SE or BBM culture microalgae Fluid nutrient medium.
  4. 4. in accordance with the method for claim 1, it is characterised in that：It is as follows that kelvin intends preparing for chlorella FSH-Y3 seed liquors： The PH of culture medium is adjusted to 10~12, is 20~30 DEG C, periodicity of illumination 24h in temperature, brightness time ratio is 14:10, light Under conditions of being 2000~20000Lux according to intensity, shaken cultivation to exponential phase.
  5. 5. according to the method described in claim 1 or 4, it is characterised in that：Step（1）Add CN201310537896.2 institutes simultaneously The scenedesmus obliquus stated（Scenedesmus obliqnus）FSH-Y2 seed liquors, the preparation method of scenedesmus obliquus FSH-Y2 seed liquors Intend chlorella FSH-Y3 with kelvin, addition is that the volume ratio of seed liquor and culture medium is 1:40~1:10.
  6. 6. in accordance with the method for claim 1, it is characterised in that：Grid algae MH-04 seed liquors, single needle algae SS-B1 seed liquors Preparation method is as follows：The PH of micro-algae culture medium is adjusted to 7~9, is 20~30 DEG C, periodicity of illumination 24h in temperature, during brightness Between compare be 14:10, intensity of illumination is 2000~20000Lux, shaken cultivation to exponential phase.
  7. 7. in accordance with the method for claim 1, it is characterised in that：Preparing for algae fibre SS-B7 seed liquors is as follows：By culture medium PH be adjusted to 7~9, temperature be 20~30 DEG C, periodicity of illumination 24h, brightness time ratio be 14:10, intensity of illumination is 2000~10000Lux, shaken cultivation to exponential phase.
  8. 8. in accordance with the method for claim 1, it is characterised in that：Control total inoculum concentration of microalgae seed liquor overall for culture medium Long-pending 5%~30%, wherein kelvin intend chlorella FSH-Y3 seed liquors, grid algae MH-04 seed liquors and/or single needle algae SS-B1 seeds Liquid, the volume ratio of algae fibre SS-B7 seed liquor threes are 1:6:6~4:1:1.
  9. 9. according to the method described in claim 1 or 8, it is characterised in that：Grid algae MH-04 seed liquors and single needle algae are inoculated with when simultaneously During SS-B1 seed liquors, the volume ratio of the two is 5:1-1:5.
  10. 10. in accordance with the method for claim 1, it is characterised in that：Described flue gas burns tail from sulfur recovery facility Gas, catalytic cracked regenerated tail gas or S-zorb regeneration tail gas.
  11. 11. according to the method described in claim 1 or 10, it is characterised in that：CO in the flue gas2Content is 5v%~45v%, SO2 Content is no more than 600 × 10-6（v/v）, NOx content is no more than 500 × 10-6（v/v）.
  12. 12. in accordance with the method for claim 1, it is characterised in that：The temperature of microalgae mixed culture is 20~30 DEG C, illumination week Phase is 24h, and brightness time ratio is 14:10, intensity of illumination is 2000~10000Lux, and culture to growth stationary phase terminates.
CN201610386730.9A 2016-06-05 2016-06-05 Method for producing microalgae grease by using flue gas Active CN107460216B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610386730.9A CN107460216B (en) 2016-06-05 2016-06-05 Method for producing microalgae grease by using flue gas

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610386730.9A CN107460216B (en) 2016-06-05 2016-06-05 Method for producing microalgae grease by using flue gas

Publications (2)

Publication Number Publication Date
CN107460216A true CN107460216A (en) 2017-12-12
CN107460216B CN107460216B (en) 2019-12-13

Family

ID=60544766

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610386730.9A Active CN107460216B (en) 2016-06-05 2016-06-05 Method for producing microalgae grease by using flue gas

Country Status (1)

Country Link
CN (1) CN107460216B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753621A (en) * 2018-05-30 2018-11-06 昆明理工大学 A method of based on molasses give up mash improve single needle algae oil and fat accumulation
CN111100885A (en) * 2018-10-26 2020-05-05 中国石油化工股份有限公司 Method for improving oil production of microalgae
CN111100883A (en) * 2018-10-26 2020-05-05 中国石油化工股份有限公司 Method for producing microalgae grease by using flue gas
CN112725388A (en) * 2019-10-28 2021-04-30 中国石油化工股份有限公司 Method for producing microalgae grease by using flue gas
CN114426928A (en) * 2020-10-28 2022-05-03 中国石油化工股份有限公司 Microalgae culture method for inhibiting mixed bacteria
CN114480131A (en) * 2020-10-28 2022-05-13 中国石油化工股份有限公司 Open type culture method of oil-producing microalgae

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101918110A (en) * 2007-09-06 2010-12-15 理查德·艾伦·哈斯 Means for sequestration and conversion of COx and NOx, CONOx
CN101914572A (en) * 2010-07-20 2010-12-15 中国科学院广州能源研究所 Energy utilization method of carbon dioxide zero-emission type organic waste
CN102703326A (en) * 2012-02-13 2012-10-03 青岛理工大学 Microalga strain with high CO2 tolerance and high fixation rate and breeding method thereof
US20140356931A1 (en) * 2013-05-29 2014-12-04 Centre De Recherche Industrielle Du Quebec Method and system for the culture of microalgae
CN104450525A (en) * 2013-09-17 2015-03-25 中国石油化工股份有限公司 Combined method for microalgae breeding and denitration of exhaust gas
CN104611228A (en) * 2013-11-05 2015-05-13 中国石油化工股份有限公司 Highly oil-containing monoraphidium and culture and application thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101918110A (en) * 2007-09-06 2010-12-15 理查德·艾伦·哈斯 Means for sequestration and conversion of COx and NOx, CONOx
CN101914572A (en) * 2010-07-20 2010-12-15 中国科学院广州能源研究所 Energy utilization method of carbon dioxide zero-emission type organic waste
CN102703326A (en) * 2012-02-13 2012-10-03 青岛理工大学 Microalga strain with high CO2 tolerance and high fixation rate and breeding method thereof
CN102703326B (en) * 2012-02-13 2014-02-19 青岛理工大学 Microalga strain with high CO2 tolerance and high fixation rate and breeding method thereof
US20140356931A1 (en) * 2013-05-29 2014-12-04 Centre De Recherche Industrielle Du Quebec Method and system for the culture of microalgae
CN104450525A (en) * 2013-09-17 2015-03-25 中国石油化工股份有限公司 Combined method for microalgae breeding and denitration of exhaust gas
CN104611228A (en) * 2013-11-05 2015-05-13 中国石油化工股份有限公司 Highly oil-containing monoraphidium and culture and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HIROYO MATSUMOTO等: "Influence of CO2,SO2 and NO in Flue Gas on Microalgae Productivity", 《JOURNALOF CHEMICAL ENGINEERING OF JAPAN》 *
张硕: "耐受高浓度CO2、NO能源微藻的筛选及生长条件的优化", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 *
黄云: "微藻固定烟气高浓度CO2的藻种改良和过程优化调控", 《中国博士学位论文全文数据库 工程科技Ⅰ辑》 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753621A (en) * 2018-05-30 2018-11-06 昆明理工大学 A method of based on molasses give up mash improve single needle algae oil and fat accumulation
CN111100885A (en) * 2018-10-26 2020-05-05 中国石油化工股份有限公司 Method for improving oil production of microalgae
CN111100883A (en) * 2018-10-26 2020-05-05 中国石油化工股份有限公司 Method for producing microalgae grease by using flue gas
CN111100883B (en) * 2018-10-26 2022-08-12 中国石油化工股份有限公司 Method for producing microalgae grease by using flue gas
CN112725388A (en) * 2019-10-28 2021-04-30 中国石油化工股份有限公司 Method for producing microalgae grease by using flue gas
CN112725388B (en) * 2019-10-28 2022-05-03 中国石油化工股份有限公司 Method for producing microalgae grease by using flue gas
CN114426928A (en) * 2020-10-28 2022-05-03 中国石油化工股份有限公司 Microalgae culture method for inhibiting mixed bacteria
CN114480131A (en) * 2020-10-28 2022-05-13 中国石油化工股份有限公司 Open type culture method of oil-producing microalgae
CN114480131B (en) * 2020-10-28 2023-07-04 中国石油化工股份有限公司 Open culture method of oleaginous microalgae
CN114426928B (en) * 2020-10-28 2023-07-04 中国石油化工股份有限公司 Microalgae culture method for inhibiting mixed bacteria

Also Published As

Publication number Publication date
CN107460216B (en) 2019-12-13

Similar Documents

Publication Publication Date Title
CN105713950B (en) A method of microalgae grease is produced using flue gas
CN109576315B (en) Method for producing microalgae grease by using flue gas
CN107460216A (en) A kind of method that microalgae grease is produced using flue gas
CN104611228B (en) Highly oil-containing monoraphidium and culture and application thereof
CN105648023B (en) A kind of method that microalgae mixed culture prepares grease
CN111100885B (en) Method for improving oil production of microalgae
CN106467896B (en) A kind of kelvin being resistant to high PH intends chlorella and its culture application
CN109576314B (en) Method for preparing microalgae grease through mixed culture
CN102268377A (en) Method for improving lipid producing microalga biomass and lipid accumulation with two stage culture strategy of mixotrophic and nitrogen-rich-nitrogen-deficient culture
CN107460215A (en) A kind of method of microalgae mixed culture production grease
CN111100883B (en) Method for producing microalgae grease by using flue gas
CN106467897B (en) A kind of grease-contained scenedesmus of richness and its culture application
CN104388315A (en) Scnedesmus quadricauda for efficiently treating typical domestic sewage, and culture method and application thereof
CN104593265B (en) One plant height oil-producing scenedesmus and its screening, cultural method and application
CN105713836B (en) The grease-contained algae fibre of one plant of richness and its culture application
CN107177505A (en) One plant of grid algae and its cultural method and application
CN109880856B (en) Open type microalgae grease production method
WO2015085631A1 (en) Method for culturing botryococcus spp. with high yield
CN107460217A (en) A kind of method that mixed culture prepares microalgae grease
CN105713935B (en) A kind of method of microalgae mixed culture production grease
CN105713934B (en) A method of producing microalgae grease
CN104232559B (en) The method of cultivating microalgae and the method for producing grease
CN105713951B (en) Method for preparing microalgae grease
WO2012006302A1 (en) Cultivation of green algae chlorococcum pamirum for production of biofuel
CN109504716A (en) A kind of cultural method promoting scenedesmus obliquus oil-producing

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant