CN108753621A - A method of based on molasses give up mash improve single needle algae oil and fat accumulation - Google Patents
A method of based on molasses give up mash improve single needle algae oil and fat accumulation Download PDFInfo
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- CN108753621A CN108753621A CN201810536914.8A CN201810536914A CN108753621A CN 108753621 A CN108753621 A CN 108753621A CN 201810536914 A CN201810536914 A CN 201810536914A CN 108753621 A CN108753621 A CN 108753621A
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- 235000013379 molasses Nutrition 0.000 title claims abstract description 27
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000009825 accumulation Methods 0.000 title claims abstract description 13
- 239000004519 grease Substances 0.000 claims abstract description 19
- 239000003225 biodiesel Substances 0.000 claims abstract description 18
- 238000005286 illumination Methods 0.000 claims abstract description 11
- 230000001939 inductive effect Effects 0.000 claims abstract description 10
- 238000004108 freeze drying Methods 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 238000005119 centrifugation Methods 0.000 claims abstract description 5
- 238000000227 grinding Methods 0.000 claims abstract description 5
- 238000010790 dilution Methods 0.000 claims abstract description 3
- 239000012895 dilution Substances 0.000 claims abstract description 3
- 239000003960 organic solvent Substances 0.000 claims abstract description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 27
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 4
- 230000032050 esterification Effects 0.000 claims description 4
- 238000005886 esterification reaction Methods 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- WCYAALZQFZMMOM-UHFFFAOYSA-N methanol;sulfuric acid Chemical compound OC.OS(O)(=O)=O WCYAALZQFZMMOM-UHFFFAOYSA-N 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000004576 sand Substances 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 4
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 claims 1
- 229910052801 chlorine Inorganic materials 0.000 claims 1
- 239000000460 chlorine Substances 0.000 claims 1
- 210000002752 melanocyte Anatomy 0.000 claims 1
- 239000000401 methanolic extract Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 241001597008 Nomeidae Species 0.000 abstract 1
- 238000000638 solvent extraction Methods 0.000 abstract 1
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
- 239000003921 oil Substances 0.000 description 10
- -1 amine acyl ester Chemical class 0.000 description 5
- 235000019197 fats Nutrition 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 235000019737 Animal fat Nutrition 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002283 diesel fuel Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005809 transesterification reaction Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Fats And Perfumes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a kind of method for the mash raising single needle algae oil and fat accumulation that gives up based on molasses, belongs to biodiesel technology field.The method of the invention be by molasses give up mash dilution, as culture single needle algaeMonoraphidiumThe inducing culture of sp.FXY-10, adjusting pH value are 6.8-7.0, are dispensed, sterilizing；Cultured seed is inoculated into above-mentioned culture medium, the epiphysin of various concentration is then added, carries out illumination shaking flask culture；Frustule is collected by centrifugation daily, after freeze-drying, then grinding utilizes the grease in organic solvent extraction single needle algae, biodiesel is prepared.Operation is simple by the present invention, reduces production cost, and the useless mash of molasses can be utilized to effectively improve oil and fat accumulation under the action of derivant, while can also reduce the environmental pollution in the useless mash processing procedure of molasses.
Description
Technical field
The present invention relates to it is a kind of based on molasses give up mash improve single needle algae oil and fat accumulation method, belong to biodiesel technology
Field.
Background technology
Biodiesel is refined from vegetable and animals oils, it refers to oil crops such as soybean, rape etc., Wild oil plant and work
The water plants such as Cheng Weizao grease and animal fat, food garbage oil etc. are that feedstock oil passes through transesterification or thermochemical processes system
At the reproducibility diesel fuel instead of petrifaction diesel.Biodiesel is one kind of biomass energy, in physical property with
Petrifaction diesel is close, but chemical composition is different.
The conventional method of biodiesel is prepared using microalgae, not only production cost is high, and fat content and lipid-producing are low, and
And be difficult to apply to mass produce, change on the basis of the advantages such as the present invention has photosynthetic efficiency high in microalgae, and growth cycle is short
Traditional microdisk electrode mode is become, has given up mash as basic culture medium using molasses, can both solve the useless mash sewage of molasses
The problem of processing, and the nutritional ingredient culture microalgae in the useless mash of molasses can be utilized, while grease in microalgae can be improved again
Accumulation.And after adding epiphysin in microalgae, further microalgae grease can be promoted to accumulate.
Invention content
The object of the present invention is to provide it is a kind of based on molasses give up mash joint epiphysin improve single needle algae oil and fat accumulation side
Method, this method process is simple, and production cost is relatively low, specifically includes following steps：
（1）Prepare inducing culture：By molasses give up mash dilution, as culture single needle algaeMonoraphidium sp. FXY-10
Inducing culture, adjusting pH value be 6.8-7.0, dispense, sterilizing.
（2）Frustule is induced to accumulate grease：Cultured seed is inoculated into above-mentioned culture medium, various concentration is then added
Epiphysin, carry out illumination shaking flask culture.
（3）Prepare biodiesel：Frustule is collected by centrifugation daily, after freeze-drying, then grinding utilizes organic solvent to extract single
Grease in needle algae, is prepared biodiesel.
Preferably, step of the present invention（1）The useless mash of middle molasses dilutes 3200 times.
Preferably, in culture medium of the present invention epiphysin a concentration of 10-4~10-1μm ol/L, condition of culture are：Temperature 25
DEG C, intensity of illumination 3500lux, 150 r/min of shaking speed.
Preferably, step of the present invention（3）It is middle prepare biodiesel method be specially：By frustule medium centrifugal
It collects, condition 3500r/min, centrifuges 10 min, and freeze-drying is centrifuged after being washed twice repeatedly with distilled water；Add freeze-dried algae powder
Chloroform/methanol is used after 2 times of quality quarts sand grindings（2:1,v/v）Grease is extracted, repeats to collect extracting solution concentration after extracting 2 times,
Said extracted grease, which adds sulfuric acid-methanol, to carry out esterification and is prepared biodiesel, and the volume ratio of sulfuric acid and methanol is 3:97.
The beneficial effects of the invention are as follows：
（1）The present invention utilizes epiphysin, reduces production cost；The useless mash of molasses realizes zero-emission, is conducive to environmental protection, will
The culture of environmental pollution treatment and microalgae is combined together, and is turned waste into wealth.
（2）Compared with the control group, the fat content for adding epiphysin is increased to 52.27% to the present invention.
（3）Single needle algae can grow in the molasses for having added epiphysin give up mash culture medium well in the present invention, not only solve
The process problem of the useless mash of molasses of having determined, while improving lipid-producing.
Description of the drawings
Fig. 1 is the variation diagram of various concentration epiphysin and the lower single needle algae oil fat content of amine acyl ester effect.
Specific implementation mode
Invention is further described in detail with reference to the drawings and specific embodiments, but the scope of the present invention is not limited to
The content.
Comparative example 1
By molasses give up mash dilute 3200 times, add the epiphysin of 0mg/L, as culture single needle algae inducing culture, adjust
PH value is between 6.8-7.0；121 DEG C of sterilizing 20min of high temperature and pressure, access single needle algae seed liquor, frustule initial inoculum is
1g/L, 25 DEG C, intensity of illumination 3500lux, shaking speed 150r/min of cultivation temperature carry out illumination shaking flask culture.
As a result：The control group fat content is 39.2%.
Comparative example 2
（1）Prepare inducing culture：By molasses give up mash dilute 3200 times, as single needle algaeMonoraphidium sp. FXY-
10 inducing culture, then be added amine acyl ester, adjust pH value between 6.8-7.0, packing, sterilising conditions be 121 DEG C, 20
min；Amine acyl ester concentration is respectively 100 μm of ol/L, 10 μm of ol/L, 1 μm of ol/L, 10-1μm ol/L, 10-2μmol/L。
（2）Frustule is induced to accumulate grease：It will utilize and foster cultured single needle algae of BG-11 are inoculated into above-mentioned culture
Base carries out illumination shaking flask culture；Condition of culture is：25 DEG C of temperature, 3500 lux of intensity of illumination, 150 r/min of shaking speed.
（3）Prepare biodiesel：Frustule is collected by centrifugation daily, frustule medium centrifugal is collected, condition 3500
R/min centrifuges 10 min, and centrifuges freeze-drying after being washed twice repeatedly with distilled water；Addition 2 times of quality quarts sand of freeze-dried algae powder are ground
Then mill uses the mixed solution of chloroform and methanol（Chloroform, methanol volume ratio are 2:1）Grease is extracted, repeats to collect after extracting 2 times
Extracting solution concentrates, and the grease addition sulfuric acid-methanol of extraction carries out esterification and biodiesel, the volume of sulfuric acid and methanol is prepared
Than being 3:97.
Fat content under different amine acyl ester (DA-6) concentration conditions of table 2
Embodiment 1
（1）Prepare inducing culture：By molasses give up mash dilute 3200 times, as single needle algaeMonoraphidium sp. FXY-
10 inducing culture, is then added epiphysin, adjusts pH value between 6.8-7.0, and packing, sterilising conditions be 121 DEG C, 20
min；Epiphysin concentration is respectively 10-1μmol/L、10-2μmol/L、10-3μmol/L、10-4μmol/L。
（2）Frustule is induced to accumulate grease：It will utilize and foster cultured single needle algae of BG-11 are inoculated into above-mentioned culture
Base carries out illumination shaking flask culture；Condition of culture is：25 DEG C, intensity of illumination 3500lux of temperature, 150 r/min of shaking speed.
（3）Prepare biodiesel：Frustule is collected by centrifugation daily, frustule medium centrifugal is collected, condition 3500
R/min centrifuges 10 min, and centrifuges freeze-drying after being washed twice repeatedly with distilled water；Addition 2 times of quality quarts sand of freeze-dried algae powder are ground
Then mill uses the mixed solution of chloroform and methanol（Chloroform, methanol volume ratio are 2:1）Grease is extracted, repeats to collect after extracting 2 times
Extracting solution concentrates, and the grease addition sulfuric acid-methanol of extraction carries out esterification and biodiesel, the volume of sulfuric acid and methanol is prepared
Than being 3:97.
Fat content under different epiphysin (MT) concentration conditions of table 1
The result shows that：Mash is given up as basic culture medium induction single needle algae accumulation grease with molasses, and addition external source epiphysin can carry
The lipid-producing of high single needle algae, and adding external source amine acyl ester does not have remarkable result, when epiphysin a concentration of 10-3It is single when μm ol/L
The fat content of needle algae improves 19.83% than control group；Illustrate that epiphysin is added in molasses give up mash can effectively improve single needle
The oil and fat accumulation of algae.
Claims (4)
1. a kind of method for the mash raising single needle algae oil and fat accumulation that given up based on molasses, which is characterized in that specifically include following steps：
（1）Prepare inducing culture：By molasses give up mash dilution, as culture single needle algaeMonoraphidium sp. FXY-10
Inducing culture, adjusting pH value be 6.8-7.0, dispense, sterilizing；
（2）Frustule is induced to accumulate grease：Cultured seed is inoculated into above-mentioned culture medium, taking off for various concentration is then added
Melanocyte carries out illumination shaking flask culture；
（3）Prepare biodiesel：Frustule is collected by centrifugation daily, after freeze-drying, then grinding utilizes organic solvent to extract single needle algae
Interior grease, is prepared biodiesel.
2. the method for the mash raising single needle algae oil and fat accumulation according to claim 1 that given up based on molasses, it is characterised in that：Step
Suddenly（1）The useless mash of middle molasses dilutes 3200 times.
3. the method for the mash raising single needle algae oil and fat accumulation according to claim 1 that given up based on molasses, it is characterised in that：Training
Support a concentration of 10 of epiphysin in base-4~10-1μm ol/L, condition of culture are：25 DEG C, intensity of illumination 3500lux of temperature, shaking table turn
150 r/min of speed.
4. the method for the mash raising single needle algae oil and fat accumulation according to claim 1 that given up based on molasses, it is characterised in that：Institute
State step（3）It is middle prepare biodiesel method be specially：Frustule medium centrifugal is collected, condition 3500r/min, from
10 min of the heart, and centrifuge freeze-drying after being washed twice repeatedly with distilled water；Chlorine is used after the 2 times of quality quarts sand grindings of addition freeze-dried algae powder
Imitative/methanol extracts grease, repeats to collect extracting solution concentration after extracting 2 times, said extracted grease adds sulfuric acid-methanol and carries out esterification
It is prepared biodiesel, the volume ratio of sulfuric acid and methanol is 3:97.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810536914.8A CN108753621A (en) | 2018-05-30 | 2018-05-30 | A method of based on molasses give up mash improve single needle algae oil and fat accumulation |
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| CN201810536914.8A CN108753621A (en) | 2018-05-30 | 2018-05-30 | A method of based on molasses give up mash improve single needle algae oil and fat accumulation |
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| CN108753621A true CN108753621A (en) | 2018-11-06 |
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Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003060807A2 (en) * | 2001-12-21 | 2003-07-24 | Triad Therapeutics, Inc. | Methods for determining polypeptide structure, function or pharmacophore from comparison of polypeptide sequences |
| EP1499303A4 (en) * | 2002-04-10 | 2007-07-25 | Fred H Miller | Multi-phase, multi-compartment capsular system |
| WO2008154294A1 (en) * | 2007-06-07 | 2008-12-18 | Unicity International, Inc. | Method and composition for providing controlled delivery of biologically active substances |
| WO2012036758A1 (en) * | 2010-09-19 | 2012-03-22 | Alex Yuan | Aquaculture biocide methods and compositions |
| US20140308248A1 (en) * | 2011-09-14 | 2014-10-16 | Vincent C. Giampapa | Dietary Supplement System for Multifunctional Anti-Aging Management and Method of Use |
| CN104470946A (en) * | 2012-03-26 | 2015-03-25 | 普罗努塔利亚公司 | Charged nutritive proteins and methods |
| CN105039437A (en) * | 2015-07-01 | 2015-11-11 | 昆明理工大学 | Method using molasses, alcohol, and waste undecanted wine to culture monoraphidium to produce diesel oil |
| CN105671095A (en) * | 2016-02-27 | 2016-06-15 | 昆明理工大学 | Cultivation method for promoting rapid accumulation of oil producing microalgae cells and grease |
| CN105803010A (en) * | 2016-03-30 | 2016-07-27 | 昆明理工大学 | Method for oil accumulation based on heterotrophic microalgae |
| CN106554976A (en) * | 2015-09-30 | 2017-04-05 | 中国石油化工股份有限公司 | A kind of method that utilization single needle algae produces microalgae grease |
| CN106635807A (en) * | 2015-11-04 | 2017-05-10 | 中国石油化工股份有限公司 | Oil-producing monoraphidium sp. as well as culture and application thereof |
| CN107304142A (en) * | 2016-04-18 | 2017-10-31 | 南京耀凯蔬菜土地专业合作社 | A kind of three-coloured amaranth that is favorably improved is to concentrated fertilizer of nitrogen use efficiency and preparation method thereof |
| CN107460215A (en) * | 2016-06-05 | 2017-12-12 | 中国石油化工股份有限公司 | A kind of method of microalgae mixed culture production grease |
| CN107460216A (en) * | 2016-06-05 | 2017-12-12 | 中国石油化工股份有限公司 | A kind of method that microalgae grease is produced using flue gas |
| CN107475171A (en) * | 2017-08-15 | 2017-12-15 | 昆明理工大学 | Application of the epiphysin in oil-producing microalgae fat content is improved |
-
2018
- 2018-05-30 CN CN201810536914.8A patent/CN108753621A/en active Pending
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003060807A2 (en) * | 2001-12-21 | 2003-07-24 | Triad Therapeutics, Inc. | Methods for determining polypeptide structure, function or pharmacophore from comparison of polypeptide sequences |
| EP1499303A4 (en) * | 2002-04-10 | 2007-07-25 | Fred H Miller | Multi-phase, multi-compartment capsular system |
| WO2008154294A1 (en) * | 2007-06-07 | 2008-12-18 | Unicity International, Inc. | Method and composition for providing controlled delivery of biologically active substances |
| WO2012036758A1 (en) * | 2010-09-19 | 2012-03-22 | Alex Yuan | Aquaculture biocide methods and compositions |
| US20140308248A1 (en) * | 2011-09-14 | 2014-10-16 | Vincent C. Giampapa | Dietary Supplement System for Multifunctional Anti-Aging Management and Method of Use |
| CN104470946A (en) * | 2012-03-26 | 2015-03-25 | 普罗努塔利亚公司 | Charged nutritive proteins and methods |
| CN105039437A (en) * | 2015-07-01 | 2015-11-11 | 昆明理工大学 | Method using molasses, alcohol, and waste undecanted wine to culture monoraphidium to produce diesel oil |
| CN106554976A (en) * | 2015-09-30 | 2017-04-05 | 中国石油化工股份有限公司 | A kind of method that utilization single needle algae produces microalgae grease |
| CN106635807A (en) * | 2015-11-04 | 2017-05-10 | 中国石油化工股份有限公司 | Oil-producing monoraphidium sp. as well as culture and application thereof |
| CN105671095A (en) * | 2016-02-27 | 2016-06-15 | 昆明理工大学 | Cultivation method for promoting rapid accumulation of oil producing microalgae cells and grease |
| CN105803010A (en) * | 2016-03-30 | 2016-07-27 | 昆明理工大学 | Method for oil accumulation based on heterotrophic microalgae |
| CN107304142A (en) * | 2016-04-18 | 2017-10-31 | 南京耀凯蔬菜土地专业合作社 | A kind of three-coloured amaranth that is favorably improved is to concentrated fertilizer of nitrogen use efficiency and preparation method thereof |
| CN107460215A (en) * | 2016-06-05 | 2017-12-12 | 中国石油化工股份有限公司 | A kind of method of microalgae mixed culture production grease |
| CN107460216A (en) * | 2016-06-05 | 2017-12-12 | 中国石油化工股份有限公司 | A kind of method that microalgae grease is produced using flue gas |
| CN107475171A (en) * | 2017-08-15 | 2017-12-15 | 昆明理工大学 | Application of the epiphysin in oil-producing microalgae fat content is improved |
Non-Patent Citations (8)
| Title |
|---|
| XUNZAN DONG等: ""Enhancement of lipid production and nutrient removal of Monoraphidium sp. FXY-10 by combined melatonin and molasses wastewater treatment"", 《JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS》 * |
| Y. SATYAWALI等: ""Wastewater treatment in molasses-based alcohol distilleries for COD and color removal: A review"", 《JOURNAL OF ENVIRONMENTAL MANAGEMENT》 * |
| 宋雪婷等: ""外源褪黑素对缺氮胁迫下单针藻Monoraphidium sp．QLY-1 中油脂合成的影响"", 《中国油脂》 * |
| 李喜明等: ""褪黑素调控缺氮胁迫下单针藻中油脂积累的影响"", 《食品与发酵工业》 * |
| 李大菲等: ""褪黑素对单针藻油脂积累的影响"", 《水生生物学报》 * |
| 范艳霞等: ""糖蜜酒精废水生物处理方法与研究进展"", 《水处理技术》 * |
| 董训赞等: ""褪黑素诱导糖蜜废醪液培养单针藻Monoraphidium sp. FXY-10产生物燃料"", 《化工进展》 * |
| 车绕琼等: ""黄腐酸对单针藻生长和油脂含量的影响"", 《水生生物学报》 * |
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