CN108587918A - A method of it is accumulated based on simultaneous foster microalgae grease - Google Patents
A method of it is accumulated based on simultaneous foster microalgae grease Download PDFInfo
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- CN108587918A CN108587918A CN201810537226.3A CN201810537226A CN108587918A CN 108587918 A CN108587918 A CN 108587918A CN 201810537226 A CN201810537226 A CN 201810537226A CN 108587918 A CN108587918 A CN 108587918A
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- 239000004519 grease Substances 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 21
- 241000195493 Cryptophyta Species 0.000 claims abstract description 32
- 239000001963 growth medium Substances 0.000 claims abstract description 19
- 238000009825 accumulation Methods 0.000 claims abstract description 16
- 239000003225 biodiesel Substances 0.000 claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000002609 medium Substances 0.000 claims abstract description 12
- 239000007640 basal medium Substances 0.000 claims abstract description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 9
- 239000008103 glucose Substances 0.000 claims abstract description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 8
- 230000005526 G1 to G0 transition Effects 0.000 claims abstract description 5
- 239000012452 mother liquor Substances 0.000 claims abstract description 5
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 27
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- 238000005286 illumination Methods 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 230000006698 induction Effects 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- WCYAALZQFZMMOM-UHFFFAOYSA-N methanol;sulfuric acid Chemical compound OC.OS(O)(=O)=O WCYAALZQFZMMOM-UHFFFAOYSA-N 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 239000012074 organic phase Substances 0.000 claims description 4
- 239000012071 phase Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000004576 sand Substances 0.000 claims description 4
- 239000002283 diesel fuel Substances 0.000 claims description 3
- 230000032050 esterification Effects 0.000 claims description 3
- 238000005886 esterification reaction Methods 0.000 claims description 3
- 239000011044 quartzite Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims 1
- 238000010790 dilution Methods 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 229910052564 epsomite Inorganic materials 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 239000002699 waste material Substances 0.000 description 9
- 239000002028 Biomass Substances 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 230000012010 growth Effects 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 3
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000000446 fuel Substances 0.000 description 2
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 description 2
- 229960003987 melatonin Drugs 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000019737 Animal fat Nutrition 0.000 description 1
- 206010013082 Discomfort Diseases 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000719329 Trentepohlia Species 0.000 description 1
- 230000005791 algae growth Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 239000005712 elicitor Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 230000007773 growth pattern Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 150000004668 long chain fatty acids Chemical class 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 238000005809 transesterification reaction Methods 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a kind of methods based on simultaneous foster microalgae grease accumulation, belong to biodiesel technology field.The method of the invention is：Using glucose as the 11 basal medium mixotrophic cultivation single needle algaes of BG of carbon source, wait for that single needle algae grows to exponential phase later stage collection frustule；Minimal medium after useless mash is diluted as single needle algae, is then added MgSO into basal medium4·7H2O；PH value is adjusted to 6.8 7.1, is configured to culture medium；Gained culture medium is subjected to high pressure-temperature sterilizing, accesses oil-producing single needle algae, a concentration of 0.9 1g/L of frustule, then addition absolute ethyl alcohol dissolve epiphysin mother liquor, the ultimate density of epiphysin is 10 in culture medium‑1mol/L‑10‑4Mol/L, and algae solution is placed under intense light irradiation and is cultivated；The microalgae of culture to stationary phase is enriched with, grease is extracted, biodiesel is prepared.The present invention is simple and practicable, production cost can be greatly reduced, and dramatically increases grease yield, to substantially increase the efficiency that microalgae produces biodiesel.
Description
Technical field
The invention belongs to biodiesel technology fields, and in particular to and it is a kind of to support BG-11 medium culture microalgaes using simultaneous, it
The method for utilizing the induction microalgae grease accumulation of molasses alcohol waste mash autotrophy afterwards.
Background technology
It is a kind of long chain fatty acids monoalkyl ester that biodiesel, which is commonly called as sunlight fuel, is with vegetable fat, animal fat, each
Kind waste grease and microbial grease etc. are raw material, and obtain one is prepared by transesterification reaction with short chain alcohol (such as methanol, ethyl alcohol)
Kind can replace the oxygen-containing reproducibility clean fuel of petrifaction diesel.Compared with common diesel, it have it is environmentally friendly, renewable, degradable,
The features such as discharge of post-combustion pollution object is low, greenhouse gas emission is low.
In recent years, microalgae is concerned as the raw material of production biodiesel.The growth pattern of microalgae mainly has autotrophy, different
It supports and and supports.Present microalgae mainly uses light autotrophy culture.But light autotrophy training mode have make frustule growth cycle it is long,
The features such as low yield, easy microbiological contamination and harvesting of high cost.And and can largely accumulate because have illumination and organic carbon source under the conditions of supporting
Tired biomass, shortens frustule growth cycle.But simultaneous foster Biomass yield and high-quality yield are relatively low, therefore and foster general discomfort
In accumulation grease.
Single needle Trentepohlia is in a kind of unicellular green microalgae, it is under stress conditions, such as high temperature, intense light irradiation, nitrogen hunger etc.,
Grease can largely be accumulated.Molasses alcohol waste mash can cause to seriously endanger to water environment, but simultaneously in waste liquid containing a large amount of
Nutriment needed for crops, so it is a kind of resource of preciousness again.According to analysis, 6%-8% containing organic matter in the mash that gives up,
P2O50.02%-0.04% 、K2O 0.6%- 1.2%, total nitrogen 0.3%- 0.5%.And Yunnan Province's cane sugar factory produces the mash 156 that gives up per year
Ten thousand t are equivalent to containing organic matter about 10.9t；P2O5About 474t；K2O about 1.4t；Total nitrogen about 6243t.How to alcohol waste mash into
Ji of passing through and effective improvement, are still the problem that sugar plant pollution is administered so far.
Invention content
The purpose of the present invention is to provide a kind of methods based on simultaneous foster microalgae grease accumulation, and this method is two benches culture
Process utilizes molasses alcohol waste mash autotrophy culture microalgae grease later specially using simultaneous foster BG-11 medium culture microalgaes
The method of accumulation；Based on induction, promote the increase of microalgae grease content and biomass, this method process is simple, production cost
It is low, specifically include following steps：
（1）Using glucose as the BG-11 basal medium mixotrophic cultivation single needle algaes of carbon source, wait for that single needle algae grows to exponential phase
Later stage collects frustule.
（2）Prepare the culture medium of induction：Then minimal medium after useless mash is diluted as single needle algae is trained to basis
It supports in base and MgSO is added4·7H2O；PH value is adjusted to 6.8-7.1, is configured to culture medium.
（3）Oil-producing microalgae culture：By step（2）Gained culture medium carries out high pressure-temperature sterilizing, accesses step（1）In production
Oily single needle algae, a concentration of 0.9-1g/L of frustule, the epiphysin mother liquor that then addition absolute ethyl alcohol dissolves take off in culture medium black
The ultimate density of element is 10-1mol/L -10-4Mol/L, and algae solution is placed under intense light irradiation and is cultivated.
（4）Prepare biodiesel：The microalgae of culture to stationary phase is enriched with, grease is extracted, biodiesel is prepared.
Preferably, step of the present invention（1）The addition of glucose is 9-11g/L in middle basal medium, cultivates single needle algae
Temperature be 23-26 DEG C.
Preferably, step of the present invention（1）In give up mash dilute 3000-3200 times.
Preferably, step of the present invention（2）Middle MgSO4·7H2The addition of O is 283-293mg/L.
Preferably, step of the present invention（3）Middle condition of culture is：Illumination cultivation temperature is 23-26 DEG C, and intensity of illumination is
2500-4000lux, shaking speed 130-160r/min.
Preferably, step of the present invention（4）Prepare biodiesel the specific steps are：Medium centrifugal is enriched with, item is centrifuged
Part is that 3400-3600r/min centrifuges 8-10min, and is lyophilized after being washed repeatedly 2-3 times with distilled water, and stone is added in freeze-dried algae powder
Sand grinds 20-30min, and it is 2 then to use chloroform and methanol volume ratio:The mixed solution of 1 chloroform-methanol carries out grease and carries
It takes, repeats 3-4 rear organic phase of collecting of extraction and concentrated, the grease extracted is added sulfuric acid-methanol solution and carries out methyl esters
Biodiesel is prepared in change, and wherein sulfuric acid and methanol volume ratio are 3:97.
The beneficial effects of the invention are as follows：
（1）The present invention can save carbon source and nitrogen source, improve the utilization rate and conversion ratio of sugar, reduce production cost；Useless mash
It realizes zero-emission, is conducive to environmental protection；The processing of environmental pollution and the culture of microalgae are combined together, turned waste into wealth.
（2）The result shows that：Frustule after inducing and support using light autotrophy addition elicitor accumulates grease, it is found that addition is outer
Source epiphysin can effectively facilitate the accumulation of grease in heterotrophic microalgae cell；By the method for this two benches culture microalgae, both realized
The fast high-density growth of microalgae, and realize the high-quality culture of microalgae；When epiphysin a concentration of 10-3When μm ol/L, algae
Fat content improves 26% than control group in cell, has reached 54.85%；Biomass improves 17%, has reached 1.04g/L.
（3）Oil-producing microalgae can largely accumulate biomass inside simultaneous foster BG-11 culture mediums in the present invention.Later can
It is grown in useless mash culture medium well, not only solves problem of environmental pollution, and converted pollutant to reproducible
Bioenergy.It is that enterprise brings economic benefit that useless mash, which is used, as minimal medium culture microalgae accumulation grease, can be accomplished completely
" turning waste into wealth " has good waste utilization foreground.Microalgae can not only be grown well in useless mash, and can also be promoted
The increase of oil-producing microalgae fat content；Mechanism of action that may be present is that the nitrogen source, carbon source inside useless mash can be fine by microalgae
Ground is absorbed and is utilized, and the acting factor for promoting micro algae growth is also likely to be present inside useless mash.
Description of the drawings
Fig. 1 be in embodiment 1 in the case of melatonin content difference in microalgae cell grease accumulation.
Specific implementation mode
With reference to specific embodiment, invention is further described in detail, but protection scope of the present invention is not limited to
The content.
Embodiment 1
A method of it is accumulated based on simultaneous foster microalgae grease, specifically includes following steps：
（1）Using glucose as the BG-11 basal medium mixotrophic cultivation oil-producing microalgaes of carbon sourceMonoraphidiumsp. FXY-
10, wait for that single needle algae grows to exponential phase later stage collection frustule；The addition of glucose is 9g/L, cultivates the temperature of single needle algae
Degree is 26 DEG C.
（2）Prepare the culture medium of induction：Minimal medium as single needle algae after useless mash is diluted 3200 times, then to
MgSO is added in basal medium4·7H2O, addition 287.8mg/L；PH value is adjusted to 6.8-7.1, is configured to culture medium.
（3）Oil-producing microalgae culture：By step（2）Gained culture medium carries out high pressure-temperature sterilizing, accesses step（1）In production
Oily single needle algae, a concentration of 0.9g/L of frustule, the epiphysin mother liquor that then addition absolute ethyl alcohol dissolves, epiphysin in culture medium
Ultimate density be 10-3μm ol/L, and algae solution is placed under intense light irradiation and is cultivated；Condition of culture is：Illumination cultivation temperature is 25 DEG C,
Intensity of illumination is 3500lux, shaking speed 150r/min.
（4）Prepare biodiesel：The microalgae of culture to stationary phase is enriched with, medium centrifugal is enriched with, centrifugal condition is
3500r/min centrifuges 10min, and is lyophilized after being washed repeatedly 2-3 times with distilled water, and quartzite sand grind is added in freeze-dried algae powder
20min, it is 2 then to use chloroform and methanol volume ratio:The mixed solution of 1 chloroform-methanol carries out grease extraction, repeats to extract 3-
It collects organic phase after 4 times to be concentrated, the grease extracted is added the progress esterification of sulfuric acid-methanol solution and biology is prepared
Diesel oil, wherein sulfuric acid are 3 with methanol volume ratio:97.
Control other conditions are constant, and it is 0 μm of ol/L, 10 to make the ultimate density of epiphysin in culture medium-1μmol/L、10-2μ
mol/L、10-3μmol/L、10-4μmol/L。
Interpretation of result：In the case of melatonin content difference in microalgae cell the accumulation of grease as shown in Figure 1, can by figure
To find out, addition external source epiphysin can effectively facilitate the accumulation of grease in heterotrophic microalgae cell；Pass through two benches culture microalgae
Method, had not only realized the fast high-density growth of microalgae, but also realized the high-quality culture of microalgae；When epiphysin a concentration of 10-3μ
When mol/L, fat content improves 26% than control group in frustule, has reached 54.85%；Biomass improves 35%, reaches
1.22g/L。
Embodiment 2
A method of it is accumulated based on simultaneous foster microalgae grease, specifically includes following steps：
（1）Using glucose as the BG-11 basal medium mixotrophic cultivation oil-producing microalgaes of carbon sourceMonoraphidiumsp. FXY-
10, wait for that single needle algae grows to exponential phase later stage collection frustule；The addition of glucose is 11g/L, culture single needle algae
Temperature is 23 DEG C.
（2）Prepare the culture medium of induction：Minimal medium as single needle algae after useless mash is diluted 3250 times, then to
MgSO is added in basal medium4·7H2O, addition 293mg/L；PH value is adjusted to 6.8-7.1, is configured to culture medium.
（3）Oil-producing microalgae culture：By step（2）Gained culture medium carries out high pressure-temperature sterilizing, accesses step（1）In production
Oily single needle algae, a concentration of 1g/L of frustule, the epiphysin mother liquor that then addition absolute ethyl alcohol dissolves, epiphysin in culture medium
Ultimate density is 10-4μm ol/L, and algae solution is placed under intense light irradiation and is cultivated；Condition of culture is：Illumination cultivation temperature is 26 DEG C, light
It is 4000lux, shaking speed 160r/min according to intensity.
（4）Prepare biodiesel：The microalgae of culture to stationary phase is enriched with, medium centrifugal is enriched with, centrifugal condition is
3600r/min centrifuges 8min, and is lyophilized after being washed repeatedly 2-3 times with distilled water, addition quartzite sand grind 20- in freeze-dried algae powder
30min, it is 2 then to use chloroform and methanol volume ratio:The mixed solution of 1 chloroform-methanol carries out grease extraction, repeats to extract 3-
It collects organic phase after 4 times to be concentrated, the grease extracted is added the progress esterification of sulfuric acid-methanol solution and biology is prepared
Diesel oil, wherein sulfuric acid are 3 with methanol volume ratio:97；Fat content improves 22% than control group in frustule in the present embodiment,
Reach 51.15%；Biomass improves 25%, has reached 1.12g/L.
Claims (6)
1. a kind of method based on simultaneous foster microalgae grease accumulation, which is characterized in that include the following steps：
（1）Using glucose as the BG-11 basal medium mixotrophic cultivation single needle algaes of carbon source, wait for that single needle algae grows to exponential phase
Later stage collects frustule；
（2）Prepare the culture medium of induction：Minimal medium after useless mash is diluted as single needle algae, then to basal medium
Middle addition MgSO4·7H2O；PH value is adjusted to 6.8-7.1, is configured to culture medium；
（3）Oil-producing microalgae culture：By step（2）Gained culture medium carries out high pressure-temperature sterilizing, accesses step（1）In oil-producing list
Needle algae, a concentration of 0.9-1g/L of frustule, the epiphysin mother liquor that then addition absolute ethyl alcohol dissolves, epiphysin in culture medium
Ultimate density is 10-1mol/L -10-4Mol/L, and algae solution is placed under intense light irradiation and is cultivated；
（4）Prepare biodiesel：The microalgae of culture to stationary phase is enriched with, grease is extracted, biodiesel is prepared.
2. the method according to claim 1 based on simultaneous foster microalgae grease accumulation, it is characterised in that：Step（1）Middle basis training
The addition for supporting glucose in base is 9-11g/L, and the temperature of culture single needle algae is 23-26 DEG C.
3. the method according to claim 1 based on simultaneous foster microalgae grease accumulation, it is characterised in that：Step（1）In give up mash
3000-3200 times of dilution.
4. the method according to claim 1 based on simultaneous foster microalgae grease accumulation, it is characterised in that：Step（2）Middle MgSO4·
7H2The addition of O is 283-293mg/L.
5. the method according to claim 1 based on simultaneous foster microalgae grease accumulation, it is characterised in that：Step（3）Middle culture item
Part is：Illumination cultivation temperature is 23-26 DEG C, intensity of illumination 2500-4000lux, shaking speed 130-160r/min.
6. the method according to claim 1 based on simultaneous foster microalgae grease accumulation, it is characterised in that：Step（4）Prepare biology
Diesel oil the specific steps are：Medium centrifugal is enriched with, centrifugal condition is 3400-3600r/min centrifugation 8-10min, and to steam
Distilled water is lyophilized after washing repeatedly 2-3 times, and quartzite sand grind 20-30min is added in freeze-dried algae powder, then uses chloroform and methanol volume
Than being 2:The mixed solution of 1 chloroform-methanol carries out grease extraction, repeats 3-4 rear organic phase of collecting of extraction and is concentrated, is carried
The grease obtained is added the progress esterification of sulfuric acid-methanol solution and biodiesel, wherein sulfuric acid and methanol volume ratio is prepared
It is 3:97.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109321510A (en) * | 2018-09-29 | 2019-02-12 | 昆明理工大学 | Witchweed lactone is promoting the application in single needle algae oil and fat accumulation |
| CN109337816A (en) * | 2018-09-29 | 2019-02-15 | 昆明理工大学 | A method of reducing molasses alcohol waste mash total nitrogen, total phosphorus and COD |
| CN109929886A (en) * | 2019-03-18 | 2019-06-25 | 昆明理工大学 | A method of utilizing walnut shell extract culture single needle algae generation diesel oil |
| CN110218652A (en) * | 2019-05-27 | 2019-09-10 | 昆明理工大学 | A method of promoting micro algae growth and oil and fat accumulation in BG-11 culture medium |
| CN110229755A (en) * | 2019-06-17 | 2019-09-13 | 昆明理工大学 | A method of promote bloom nitrogen stress to coerce lower microalgae grease using epiphysin and accumulates |
| CN112359074A (en) * | 2020-11-27 | 2021-02-12 | 齐鲁工业大学 | Method for stimulating heterotrophic microalgae oil production by using acetic acid |
| CN115029248A (en) * | 2022-06-21 | 2022-09-09 | 昆明理工大学 | Method for improving microalgae lipid yield by utilizing recycled wastewater |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0667330A (en) * | 1992-08-19 | 1994-03-11 | Konica Corp | Production of photographic material |
| KR20030053188A (en) * | 2001-12-22 | 2003-06-28 | 학교법인조선대학교 | A method for ecological treatment of organic wastewater using alga and waterflea |
| EP1481052A4 (en) * | 2001-06-13 | 2005-11-16 | Univ Oklahoma | Hyaluronan synthase genes and expression thereof |
| EP1603936A4 (en) * | 2003-02-28 | 2007-07-25 | Antigenics Inc | Use of lectins to promote oligomerization of glycoproteins and antigenic molecules |
| KR101144090B1 (en) * | 2011-06-20 | 2012-05-24 | 재단법인 탄소순환형 차세대 바이오매스 생산전환 기술연구단 | The inhibition method of algal growth using ammonium organo-nanoclays |
| CN105039437A (en) * | 2015-07-01 | 2015-11-11 | 昆明理工大学 | Method using molasses, alcohol, and waste undecanted wine to culture monoraphidium to produce diesel oil |
| CN106916747A (en) * | 2015-12-25 | 2017-07-04 | 国家开发投资公司 | The strain of Sorokin chlorella algae and its cultural method and purposes |
| CN107326039A (en) * | 2017-02-21 | 2017-11-07 | 西北大学 | A kind of AANAT gene plants binary expression vector and its construction method for being used to convert Chlamydomonas reinhardtii |
| CN107365826A (en) * | 2017-09-18 | 2017-11-21 | 深圳市德和生物科技有限公司 | A kind of method of regulating astaxanthin accumulation |
| CN107418993A (en) * | 2017-08-15 | 2017-12-01 | 昆明理工大学 | Application of the epiphysin in Determination of Astaxanthin in Haematococcus Pluvialis content is improved |
| CN107475171A (en) * | 2017-08-15 | 2017-12-15 | 昆明理工大学 | Application of the epiphysin in oil-producing microalgae fat content is improved |
-
2018
- 2018-05-30 CN CN201810537226.3A patent/CN108587918A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0667330A (en) * | 1992-08-19 | 1994-03-11 | Konica Corp | Production of photographic material |
| EP1481052A4 (en) * | 2001-06-13 | 2005-11-16 | Univ Oklahoma | Hyaluronan synthase genes and expression thereof |
| KR20030053188A (en) * | 2001-12-22 | 2003-06-28 | 학교법인조선대학교 | A method for ecological treatment of organic wastewater using alga and waterflea |
| EP1603936A4 (en) * | 2003-02-28 | 2007-07-25 | Antigenics Inc | Use of lectins to promote oligomerization of glycoproteins and antigenic molecules |
| KR101144090B1 (en) * | 2011-06-20 | 2012-05-24 | 재단법인 탄소순환형 차세대 바이오매스 생산전환 기술연구단 | The inhibition method of algal growth using ammonium organo-nanoclays |
| CN105039437A (en) * | 2015-07-01 | 2015-11-11 | 昆明理工大学 | Method using molasses, alcohol, and waste undecanted wine to culture monoraphidium to produce diesel oil |
| CN106916747A (en) * | 2015-12-25 | 2017-07-04 | 国家开发投资公司 | The strain of Sorokin chlorella algae and its cultural method and purposes |
| CN107326039A (en) * | 2017-02-21 | 2017-11-07 | 西北大学 | A kind of AANAT gene plants binary expression vector and its construction method for being used to convert Chlamydomonas reinhardtii |
| CN107418993A (en) * | 2017-08-15 | 2017-12-01 | 昆明理工大学 | Application of the epiphysin in Determination of Astaxanthin in Haematococcus Pluvialis content is improved |
| CN107475171A (en) * | 2017-08-15 | 2017-12-15 | 昆明理工大学 | Application of the epiphysin in oil-producing microalgae fat content is improved |
| CN107365826A (en) * | 2017-09-18 | 2017-11-21 | 深圳市德和生物科技有限公司 | A kind of method of regulating astaxanthin accumulation |
Non-Patent Citations (7)
| Title |
|---|
| LI HUANG等: ""Effects of additional Mg2+ on the growth, lipid production,and fatty acid composition of Monoraphidium sp. FXY-10 under different culture conditions "", 《ANN MICROBIOL》 * |
| XUNZAN DONG等: ""Enhancement of lipid production and nutrient removal of Monoraphidium sp. FXY-10 by combined melatonin and molasses wastewater treatment"", 《JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS》 * |
| YONGTENG ZHAO等: ""Production of biomass and lipids by the oleaginous microalgae Monoraphidium sp. QLY-1 through heterotrophic cultivation and photo-chemical modulator induction"", 《BIORESOURCE TECHNOLOGY》 * |
| 李喜明等: ""褪黑素调控缺氮胁迫下单针藻中油脂积累的影响"", 《食品与发酵工业》 * |
| 李大菲等: ""褪黑素对单针藻油脂积累的影响"", 《水生生物学报》 * |
| 范艳霞等: ""糖蜜酒精废水生物处理方法与研究进展"", 《水处理技术》 * |
| 董训赞等: ""褪黑素诱导糖蜜废醪液培养单针藻Monoraphidium sp. FXY-10产生物燃料"", 《化工进展》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN109337816A (en) * | 2018-09-29 | 2019-02-15 | 昆明理工大学 | A method of reducing molasses alcohol waste mash total nitrogen, total phosphorus and COD |
| CN109929886A (en) * | 2019-03-18 | 2019-06-25 | 昆明理工大学 | A method of utilizing walnut shell extract culture single needle algae generation diesel oil |
| CN110218652A (en) * | 2019-05-27 | 2019-09-10 | 昆明理工大学 | A method of promoting micro algae growth and oil and fat accumulation in BG-11 culture medium |
| CN110229755A (en) * | 2019-06-17 | 2019-09-13 | 昆明理工大学 | A method of promote bloom nitrogen stress to coerce lower microalgae grease using epiphysin and accumulates |
| CN112359074A (en) * | 2020-11-27 | 2021-02-12 | 齐鲁工业大学 | Method for stimulating heterotrophic microalgae oil production by using acetic acid |
| CN112359074B (en) * | 2020-11-27 | 2022-10-11 | 齐鲁工业大学 | Method for stimulating heterotrophic microalgae to produce oil by using acetic acid |
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