CN114468308B - 一种利用酶解蛋白-菊粉制备抗氧化型低脂粉的方法 - Google Patents
一种利用酶解蛋白-菊粉制备抗氧化型低脂粉的方法 Download PDFInfo
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Abstract
一种利用酶解蛋白‑菊粉制备抗氧化型低脂粉的方法,属于食品加工技术领域。本发明为获得具有调节脂质消化特性的产品,将槲皮素包埋在以蛋白酶解物‑菊粉聚合物为乳化剂制备的高内相乳液中,然后通过调控高内相乳液的油相体积分数实现更高水平的槲皮素包封,并通过调控菊粉添加量实现脂质的可控消化,最后干燥处理高内相乳液获得抗氧化型低脂粉。本发明不但解决了现有运载体系稳定性差、槲皮素包封率低、游离脂肪酸释放量大等问题,而且对蛋白质‑多糖稳定高内相乳液配方的设计和制造,甚至对调控脂质消化特性的大豆蛋白产品的开发具有重要的意义。
Description
技术领域
本发明涉及一种利用酶解蛋白-菊粉制备抗氧化型低脂粉的方法,属于食品加工技术领域。
技术背景
高内相乳液是一种内相体积分数达到74%及以上的乳液体系,具有一些独特的性能,包括高含油量、大的比表面积、固体状结构和高封装水平等。近年来,高内相乳液因其生产工艺简单、传递性能优良受到了大量关注,被广泛应用于食品、制药和化妆品等行业。
游离脂肪酸,作为人体内脂肪代谢的中间产物,是机体细胞能量代谢的重要代谢底物,能够为心脏、肝脏、骨骼肌等器官代谢提供能量。但是人体内大量游离脂肪酸的产生会引起动脉粥样硬化、急性冠状综合征、高血压、心力衰竭等疾病的发生。因此,具有调节脂质消化特性的产品的设计与开发迫在眉睫。
发明内容
为克服上述现有技术的不足,获得具有调节脂质消化特性的产品,本发明提供了一种利用酶解蛋白-菊粉制备抗氧化型低脂粉的方法,该方法包括如下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;
步骤三,将槲皮素溶解在无水乙醇中,获得槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液;
步骤五,对高内相乳液进行干燥处理,获得抗氧化型低脂粉。
进一步地限定,步骤一所述蛋白酶解物溶液浓度为2%w/v。
进一步地限定,步骤二所述蛋白酶解物-菊粉混合溶液中菊粉浓度为1~7%w/v。
优选地,步骤二所述蛋白酶解物-菊粉混合溶液中菊粉浓度为5%w/v。
进一步地限定,步骤三所述槲皮素乙醇溶液的质量浓度为10mg/mL。
进一步地限定,步骤三所述槲皮素油相溶液中槲皮素与玉米油比例为0.1%w/w。
进一步地限定,步骤四所述高内相乳液中油相溶液所占的体积分数为74%~80%。
优选地,步骤四所述高内相乳液中油相溶液所占体积分数为74%。
本发明的有益效果:
本发明通过将具有抗氧化活性的蛋白酶解物与菊粉结合,可以使二者发生非共价结合,形成蛋白酶解物-多糖聚合物,形成的蛋白酶解物-多糖聚合物可在不使用其他有机溶剂和表面活性剂的条件下制备稳定性良好的高内相乳液,并通过调控油相体积实现更高水平的槲皮素的包封,以及通过调控菊粉的添加量实现高内相乳液中游离脂肪酸的可控释放,最后干燥处理高内相乳液获得兼具抗氧化能力和低脂特性的抗氧化型低脂粉。本发明不但解决了现有运载体系稳定性差、槲皮素包封率低、游离脂肪酸释放量大等问题,还可能对蛋白质-多糖稳定高内相乳液配方的设计和制造,甚至对调控脂质消化特性的大豆蛋白产品的开发具有重要的意义。
附图说明:
图1为高内相乳液模拟消化过程中游离脂肪酸的释放曲线。
具体实施方式:
下面结合具体实施方式对本发明进行详细描述。
实施例1:
一种利用酶解蛋白-菊粉制备抗氧化型低脂粉的方法,包括以下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得浓度为2%(w/v)的蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加浓度为5%(w/v)的菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;
步骤三,将槲皮素溶解在无水乙醇中,获得质量浓度为10mg/mL的槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;槲皮素油相溶液中槲皮素与玉米油比例为0.1%(w/w);
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,其中油相体积分数为74%,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液(H-74%)。该乳液平均粒径较小,具有最好的稳定性和最高的槲皮素包封率。
步骤五,将高内相乳液进行干燥处理,获得抗氧化型低脂粉。
实施例2:
一种利用酶解蛋白-菊粉制备抗氧化型低脂粉的方法,包括以下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得浓度为2%(w/v)的蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加浓度为5%(w/v)的菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;
步骤三,将槲皮素溶解在无水乙醇中,获得质量浓度为10mg/mL的槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;槲皮素油相溶液中槲皮素与玉米油比例为0.1%(w/w);
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,其中油相体积分数为76%,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液(H-76%)。与油相体积分数为74%的乳液相比,该乳液平均粒径有所增加,稳定性和槲皮素包封率有所降低。
步骤五,将高内相乳液进行干燥处理,获得抗氧化型低脂粉。
实施例3:
一种利用蛋白酶解物和菊粉制备高内相乳液的方法,包括以下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得浓度为2%(w/v)的蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加浓度为5%(w/v)的菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;
步骤三,将槲皮素溶解在无水乙醇中,获得质量浓度为10mg/mL的槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;槲皮素油相溶液中槲皮素与玉米油比例为0.1%(w/w);
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,其中油相体积分数为78%,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液(H-78%)。与油相体积分数为76%的乳液相比,该乳液平均粒径进一步增加,稳定性和槲皮素包封率进一步降低。
步骤五,将高内相乳液进行干燥处理,获得抗氧化型低脂粉。
实施例4:
一种利用蛋白酶解物和菊粉制备高内相乳液的方法,包括以下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得浓度为2%(w/v)的蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加浓度为5%(w/v)的菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;
步骤三,将槲皮素溶解在无水乙醇中,获得质量浓度为10mg/mL的槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;槲皮素油相溶液中槲皮素与玉米油比例为0.1%(w/w);
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,其中油相体积分数为80%,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液(H-80%)。该乳液具有最大的平均粒径,最差的稳定性和最低的槲皮素包封率。
步骤五,将高内相乳液进行干燥处理,获得抗氧化型低脂粉。
对实施例1-4所述方法制备获得的高内相乳液的平均粒径、电位和包封率进行测定,结果如表1所示。
表1不同油相体积分数的高内相乳液的平均粒径(nm)、电位(mV)和包封率(%)
实施例5:
一种利用蛋白酶解物和菊粉制备高内相乳液的方法,包括以下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得浓度为2%(w/v)的蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加浓度为1%(w/v)的菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;
步骤三,将槲皮素溶解在无水乙醇中,获得质量浓度为10mg/mL的槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;槲皮素油相溶液中槲皮素与玉米油比例为0.1%(w/w);
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,其中油相体积分数为74%,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液(H-1%)。与不添加菊粉的高内相乳液(HIPEs)相比,该乳液稳定性有所提高,并且在模拟小肠消化时的游离脂肪酸释放有所降低。
步骤五,将高内相乳液进行干燥处理,获得抗氧化型低脂粉。
实施例6:
一种利用蛋白酶解物和菊粉制备高内相乳液的方法,包括以下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得浓度为2%(w/v)的蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加浓度为3%(w/v)的菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;
步骤三,将槲皮素溶解在无水乙醇中,获得质量浓度为10mg/mL的槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;槲皮素油相溶液中槲皮素与玉米油比例为0.1%(w/w);
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,其中油相体积分数为74%,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液(H-3%)。与添加1%菊粉的高内相乳液相比,该乳液稳定性进一步提高,并且在模拟小肠消化时的游离脂肪酸释放进一步降低。
步骤五,将高内相乳液进行干燥处理,获得抗氧化型低脂粉。
实施例7:
一种利用蛋白酶解物和菊粉制备高内相乳液的方法,包括以下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得浓度为2%(w/v)的蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加浓度为5%(w/v)的菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;
步骤三,将槲皮素溶解在无水乙醇中,获得质量浓度为10mg/mL的槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;槲皮素油相溶液中槲皮素与玉米油比例为0.1%(w/w);
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,其中油相体积分数为74%,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液(H-5%)。与添加3%菊粉的高内相乳液相比,该乳液稳定性达到最好,并且在模拟小肠消化时的游离脂肪酸释放达到最低。
步骤五,将高内相乳液进行干燥处理,获得抗氧化型低脂粉。
实施例8:
一种利用蛋白酶解物和菊粉制备高内相乳液的方法,包括以下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得浓度为2%(w/v)的蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加浓度为7%(w/v)的菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;
步骤三,将槲皮素溶解在无水乙醇中,获得质量浓度为10mg/mL的槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;槲皮素油相溶液中槲皮素与玉米油比例为0.1%(w/w);
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,其中油相体积分数为74%,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液(H-7%)。与添加5%菊粉的高内相乳液相比,该乳液稳定性略微下降,并且模拟小肠消化时的游离脂肪酸释放略微升高。
步骤五,将高内相乳液进行干燥处理,获得抗氧化型低脂粉。
实施例5-8所述方法制备获得的高内相乳液模拟消化过程中游离脂肪酸的释放曲线如图1所示。
以上所述仅为本发明专利的较佳实施例而已,并不用以限制本发明专利,凡在本发明专利的精神和原则之内所作的任何修改、等同替换和改进等,均包含在本发明专利的保护范围之内。
Claims (4)
1.一种利用酶解蛋白-菊粉制备抗氧化型低脂粉的方法,其特征在于,包括以下步骤:
步骤一,将蛋白酶解物溶解在去离子水中,室温下搅拌至蛋白酶解物完全溶解,获得蛋白酶解物溶液;
步骤二,向蛋白酶解物溶液中添加菊粉粉末,室温下搅拌至菊粉完全溶解,获得蛋白酶解物-菊粉混合溶液;所述蛋白酶解物-菊粉混合溶液中菊粉浓度为5%w/v;
步骤三,将槲皮素溶解在无水乙醇中,获得槲皮素乙醇溶液,然后加入玉米油,在65℃下旋转蒸发,直至乙醇完全蒸发,获得槲皮素油相溶液;
步骤四,将蛋白酶解物-菊粉混合溶液与槲皮素油相溶液混合,利用高速剪切均质机在12000rpm条件下均质1min,获得高内相乳液;所述高内相乳液中油相溶液所占体积分数为74%;
步骤五,对高内相乳液进行干燥处理,获得抗氧化型低脂粉。
2.根据权利要求1所述的方法,其特征在于,步骤一所述蛋白酶解物溶液浓度为2%w/v。
3.根据权利要求1所述的方法,其特征在于,步骤三所述槲皮素乙醇溶液的质量浓度为10mg/mL。
4.根据权利要求1所述的方法,其特征在于,步骤三所述槲皮素油相溶液中槲皮素与玉米油比例为0.1%w/w。
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