CN114467945A - Grain storage protective agent containing spinosad and aggregation pheromone and application thereof - Google Patents
Grain storage protective agent containing spinosad and aggregation pheromone and application thereof Download PDFInfo
- Publication number
- CN114467945A CN114467945A CN202111429261.1A CN202111429261A CN114467945A CN 114467945 A CN114467945 A CN 114467945A CN 202111429261 A CN202111429261 A CN 202111429261A CN 114467945 A CN114467945 A CN 114467945A
- Authority
- CN
- China
- Prior art keywords
- aggregation pheromone
- agent
- pest
- grain storage
- protective agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
- A01N63/14—Insects
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Insects & Arthropods (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a stored grain protective agent containing spinosad and aggregation pheromone and application thereof, in particular to a compound composition containing spinosad and aggregation pheromone, a stored grain protective agent containing the compound composition, a preparation method of the stored grain protective agent and application of the stored grain protective agent in prevention and control of stored grain pests. The compound composition comprises coleopteran pest aggregation pheromone and/or lepidopteran pest aggregation pheromone, and is compounded with spinosad for use, so that the compound composition has the advantages of high insecticidal efficiency, quick action, convenience in operation, low use cost and the like, and can solve the problem of grain storage safety of large grain storage depots and small farmers at the same time.
Description
Technical Field
The invention relates to a compound composition containing spinosad and aggregation pheromone, a grain storage protective agent containing the compound composition, a preparation method of the grain storage protective agent and application of the grain storage protective agent in grain storage pest control, and belongs to the field of biological pesticides.
Background
People take food as the day, and nations take grain as the basis. During the process of grain storage and transportation, a large amount of loss is caused by insect pests, and according to statistics of national grain and material reserve authorities, the loss caused by the stored grain insect mold in China is up to 200 plus 300 million yuan every year. The prevention and control of the stored grain pests in China have a series of problems of continuously enhanced drug resistance of the pests, chemical agent residue, environmental pollution and the like due to the single use of phosphine for fumigation for a long time. Therefore, the development of safe, efficient, environment-friendly and grain storage pest biological control agents without influence on the quality of stored grains has become an urgent need for realizing green grain storage, ensuring food safety and protecting ecological environment.
Spinosad is a great discovery in the field of biological pesticides, is a macrolide pesticide generated by aerobic fermentation of soil actinomycete saccharopolyspora spinosa (Saccahromoso), has the safety of biological pesticides and the quick-acting property of chemical pesticides, and is a safe, high-efficiency and environment-friendly pesticide recognized in the world. Spinosyns present rapid contact and feeding toxicity to pests. Unlike traditional organophosphorus and pyrethroid insecticides, spinosad causes non-functional muscle contraction and failure by stimulating the nervous system of insects, is accompanied by trembling and paralysis, and simultaneously acts on a Y-aminobutyric acid receptor, and the action mechanism is unique; its site of action is different from that of a nicotinic insecticide; although similar in chemical structure to abamectin, the site of action is not identical. To date, no product of a certain type has been found to affect the nervous system of insects in the same way, and there has been no report on spinosyn cross-resistance. The spinosad can effectively control various stored grain pests including hymenoptera, coleoptera, diptera and the like, and has the advantages of small dosage, long lasting period, low residue, safety to human and non-target animals and the like. Under natural environment, the pleocidin can be converted into natural components such as carbon, nitrogen, hydrogen, oxygen and the like through various ways such as photodegradation, microbial degradation and the like, and is safe to the environment. The spinosad can effectively prevent and control the occurrence and harm of pests in stored grains, reduce the loss of the stored grains, reduce the residue of chemical agents in the grains and delay the occurrence and development of the drug resistance of the pests. Spinosad has been approved by the united states environmental protection agency in 2005 to be used as a grain storage protective agent, and a novel grain storage pest protective agent spinosad has also obtained registration permission in China. At present, the effective component is tried to develop new application and new products in the pesticide world after the protection period of Chinese patent (CN89109377.X), so that the value of the effective component is exerted and utilized more widely.
The insect pheromone is a small molecular substance (pheromone) secreted by an insect gland to the outside of a body, is an active substance secreted by the organism to influence the behavior and physiological activities of other members of the same or different species, has the main function of carrying out information exchange among individuals of the same or different species, and is divided into sex pheromones, aggregation pheromones, marker pheromones and the like. The pheromone belongs to a trace regulating substance, has the characteristics of trace high efficiency, sensitivity, specificity, no toxicity, no harm, environmental friendliness, no residue and the like, and meets the requirement of green ecology. The aggregation pheromone is also called as the aggregation pheromone, and animals inhabit other individuals of the same species by secreting the aggregation pheromone to inhabit together, take food together and attack xenogeneic objects, thereby forming population aggregation. At present, hundreds of insect aggregation pheromones can be commercially produced at home and abroad, and about 30 kinds of storage pests are involved, wherein the aggregation pheromones of more than ten main pests such as rice weevil, corn weevil, cornus beetle, samara beetle, tribolium castaneum, indian meal moth, and khapra beetle can be artificially synthesized at present.
Unlike conventional fumigants, the insecticidal mechanism of spinosad is contact and ingestion toxicity, and pests can only be killed by contacting or eating the pesticide. The pleocidin full-warehouse grain mixing can ensure the optimal insecticidal effect, but compared with phosphine fumigation, the pleocidin full-warehouse grain mixing has higher drug cost, so the pleocidin full-warehouse grain mixing is limited in popularization and application. The spinosad and the insect pheromone are compounded, and the pheromone is used for inducing and storing grain pests to contact or ingest the spinosad, so that the effects of reducing the application dosage of the spinosad and improving the insecticidal efficiency can be achieved. However, the existing grain storage pest control agent has the problem that only insects of a single sex can be trapped and killed when the existing grain storage pest control agent is used for attracting insects, so that the insecticidal efficiency is low. How to prepare a synergistic composition by compounding spinosad and insect aggregation pheromone to be used as a grain storage pest control medicament to solve the problems is not reported at present.
Disclosure of Invention
As a result of intensive studies, the present inventors have unexpectedly found that some insect sex pheromones (for example, homoptera insect sex pheromones, etc.) have low insecticidal efficiency because they can only lure and kill insects of a single sex or cannot achieve high insecticidal efficiency against insects of both sexes at the same time when attracting insects, and even when they are compounded with spinosad, it is difficult to achieve high insecticidal efficiency. In order to solve this problem, the present inventors have found that, unlike sex pheromones of homopterans, the use of an aggregation pheromone comprising an aggregation pheromone component that attracts coleopteran and lepidopteran pests, in combination with spinosad, can overcome the problem of achieving high insecticidal efficiency by trapping only insects of a single sex or by not achieving high insecticidal efficiency against insects of both sexes at the same time, thereby greatly improving insecticidal efficiency.
Specifically, the invention is realized by the following aspects:
in a first aspect, the present invention provides a compounded composition, wherein the compounded composition comprises spinosad and a pest aggregation pheromone, preferably, the pest aggregation pheromone comprises a coleopteran pest aggregation pheromone and/or a lepidopteran pest aggregation pheromone.
In a second aspect, the invention provides a grain storage protective agent which comprises the compound composition and one or more auxiliary agents.
In a third aspect, the invention provides a preparation method of the grain storage protective agent, which comprises the step of compounding and mixing spinosad with pest aggregation pheromone and one or more auxiliary agents, wherein preferably, the pest aggregation pheromone comprises coleopteran pest aggregation pheromone and/or lepidopteran pest aggregation pheromone.
In a fourth aspect, the invention provides the compound composition and the application of the grain storage protective agent in controlling grain storage pests.
Compared with the existing grain storage protective agent, the grain storage protective agent has the following advantages:
(1) compared with a spinosad single agent, the compound composition has an obvious synergistic effect, can achieve a better effect under the condition of reducing the dosage, and has no influence on the quality, color and grade of stored grains.
(2) The preparation process of the compound composition is simple and the production cost is low. All the raw medicines adopted by the invention belong to biological pesticides, and are green and environment-friendly; the compound composition does not use a large amount of organic solvent in the production process, has small pollution to the environment and meets the requirements of green and environment-friendly pesticides.
(3) The compound composition can overcome the problem that only insects of a single sex are trapped and killed or insects of two sexes cannot be simultaneously killed to achieve high insecticidal efficiency, so that the compound composition has the advantages of high insecticidal efficiency, quick action, convenience in operation, low use cost and the like, and can simultaneously solve the problem of grain storage safety of large grain storage depots and small farmers.
Detailed Description
For a better understanding of the present invention, the present invention is further illustrated below with reference to the following embodiments, but these embodiments should not be construed as limiting the present invention in any way. The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
Compound composition of spinosad and pest aggregation pheromone
In a first embodiment, the present invention provides a built composition comprising spinosad and a pest aggregation pheromone, preferably, the pest aggregation pheromone comprises a coleopteran pest aggregation pheromone and/or a lepidopteran pest aggregation pheromone.
The spinosad has the insecticidal mechanism of contact killing and stomach poisoning, and pests can be killed only by contacting with an insecticide. The accumulated pheromone is used for inducing pests to contact the spinosad, so that the insecticidal efficiency is higher than that of a single-use insecticide. In addition, the compound composition uses coleoptera pest aggregation pheromone and/or lepidoptera pest aggregation pheromone and pleocidin, and insects of two sexes are trapped and killed simultaneously, so that the insecticidal efficiency is greatly improved, and the problem that high insecticidal efficiency cannot be realized for the insects of the two sexes simultaneously in the prior art is solved.
In a preferred embodiment, the coleopteran pest aggregation pheromones include, but are not limited to, one or more of a rubisco aggregation pheromone, a rice weevil aggregation pheromone, a khaki aggregation pheromone, and a sabcomella serrulata aggregation pheromone; the lepidoptera pest aggregation pheromone comprises one or more of, but is not limited to, indian meal moth aggregation pheromone, wheat moth aggregation pheromone and pink moth aggregation pheromone. In a preferred embodiment, the pest aggregation pheromone is selected from one or more of the group consisting of a rubisco aggregation pheromone, a rice weevil aggregation pheromone, and a indian meal moth aggregation pheromone. In another preferred embodiment, the pest aggregation pheromone is selected from one or more of the group consisting of a rusty akage aggregation pheromone, a rice weevil aggregation pheromone, a khaki aggregation pheromone, and a sabatier aggregation pheromone. In yet another preferred embodiment, the pest aggregation pheromone is selected from one or more of a rice weevil aggregation pheromone, a khaki aggregation pheromone, a hornworm aggregation pheromone, and a indian meal moth aggregation pheromone.
In some embodiments, the mass ratio of spinosad to pest aggregation pheromone is 10:1 to 200000:1, preferably 100:1 to 10000:1, such as 200:1, 250:1 or 1000: 1.
Grain storage protective agent containing compound composition
In a second embodiment, the invention also provides a grain storage protective agent which comprises the compound composition and one or more auxiliary agents.
In a preferred embodiment, the ratio of spinosad in the grain storage protective agent is 0.5% to 20% by weight, such as 0.5% to 2% by weight or 1% to 20% by weight.
In a preferred embodiment, the proportion of the pest aggregation pheromone in the grain storage protectant is between 0.0001% and 0.1% by weight, such as between 0.0001% and 0.0005% by weight, between 0.0001% and 0.002% by weight, or between 0.0001% and 0.1% by weight.
In a further preferred embodiment, the proportion of the formulated composition in the grain storage protective agent is 0.5-21 wt%, such as 0.5-15 wt%, or 5-20 wt%.
The invention does not limit the dosage form of the grain storage protective agent, and can be the conventional dosage form in pesticide preparations, including powder, wettable powder, emulsion in water, suspending agent and the like, and the powder or the suspending agent is preferred.
In the present invention, the adjuvant is an adjuvant commonly used in pesticide formulations. In a further preferred embodiment, the adjuvant is one or more of a co-solvent, an emulsifier, a dispersant, a wetting agent, a thickener (e.g. xanthan gum), an anti-freeze agent (e.g. ethylene glycol), an anti-foaming agent (e.g. silicone oil) or a filler.
In a further preferred embodiment, the grain-storage protective agent is a powder, wherein the powder comprises: 0.5-2 wt% of pleocidin, 0.0001-0.002 wt% of pest aggregation pheromone, 0.1-0.2 wt% of nano titanium dioxide, and the balance of talcum powder to 100 wt%.
In a further preferred embodiment, the grain storage protective agent is a suspending agent, wherein the suspending agent comprises: spinosad 1-20 wt% (such as 5-20 wt%), pest aggregation pheromone 0.0001-0.1 wt% (such as 0.005-0.1 wt%), xanthan gum 0.1-3 wt% (such as 0.2-3 wt%), ethylene glycol 1-10 wt% (such as 2-10 wt%), defoaming agent 0.01-1 wt% (such as 0.05-1 wt%), and water in balance.
Preparation method of grain storage protective agent
In a third embodiment, the invention provides a preparation method of the grain storage protective agent, which comprises the step of compounding and mixing spinosad with pest aggregation pheromone and one or more auxiliary agents, wherein the pest aggregation pheromone comprises coleopteran pest aggregation pheromone and/or lepidopteran pest aggregation pheromone.
In a further preferred embodiment, the grain storage protective agent is a powder, and the preparation method of the powder comprises the following steps: mixing 0.5-2 wt% of pleocidin, 0.0001-0.002 wt% of pest aggregation pheromone and 0.1-0.2 wt% of nano titanium dioxide, supplementing to 100 wt% with talcum powder, and performing jet milling.
In a further preferred embodiment, the grain storage protective agent is a suspending agent, and the preparation method of the suspending agent comprises the following steps:
(1) mixing and stirring the crushed pleocidin, the pest aggregation pheromone, the thickening agent, the antifreezing agent and water for 20-30min, and dispersing in a high-speed shearing emulsifying machine at the speed of 5000-; and
(2) adding 0.01-1 wt% of defoaming agent, and dispersing in a high-speed shearing emulsifying machine at the speed of 1000-3000r/min for 20-30 minutes to prepare the uniform compound suspending agent.
Application of compound composition and grain storage protective agent
In a fourth embodiment, the invention also provides a compound composition of the spinosad and the pest aggregation pheromone and application of a grain storage protective agent containing the compound composition in controlling grain storage pests.
In preferred embodiments, common grain storage pests for control are such as: tribolium castaneum, rice weevil, corn weevil, rice moth, Indian meal moth, pink spot moth, etc.
In a preferred embodiment, the compound composition or the grain storage protective agent kills pests by full-warehouse application or surface application of grains.
The grain storage protective agent provided by the invention can be applied to the surface or local part of grain by adopting a powder spraying or mist spraying mode, and the agent enters the grain pile and the surface of the grain pile through the permeation and diffusion effects, so that the efficient control of grain storage pests is realized through the attraction effect of pheromone.
Examples
The present invention will be described in further detail with reference to examples. These examples are merely illustrative and should not be construed as limiting the scope of the invention. All technical solutions and modifications thereof implemented based on the above contents of the present invention fall within the scope of the present invention.
In the following examples, the% referred to means wt% unless otherwise specified.
Standard preparation method of spinosad and pheromone compound powder
0.5 to 2 percent of pleocidin, 0.0001 to 0.002 percent of pest pheromone, 0.1 to 0.2 percent of nano titanium dioxide and talcum powder are complemented to 100 percent, the raw materials are uniformly mixed according to the proportion, and are uniformly pulverized by airflow and packaged.
Standard preparation method of spinosad and pheromone compound suspending agent
The spinosad and pheromone compound suspending agent consists of spinosad, pest pheromone, organic solvent and water, and the weight percentage of each component is as follows: 1 to 20 percent of pleocidin, 0.0001 to 0.1 percent of pest pheromone, 0.1 to 3 percent of xanthan gum (thickening agent), 1 to 10 percent of glycol (antifreezing agent) and the balance of water. The preparation method comprises the following steps.
Firstly, spinosad powder is crushed by an ultramicro crusher, then spinosad original drug, pheromone thickener, antifreeze and water with the mixture amount are added into a container with a stirring paddle, stirred for 20-30min and evenly mixed, and then dispersed for 20-30min to be even in a high-speed shearing emulsifying machine at the speed of 5000-8000 r/min. Then 0.01-1% of defoaming agent is added, and dispersed for 20-30 minutes in a high-speed shearing emulsifying machine at the speed of 1000-3000r/min to prepare the uniform compound suspending agent.
Example 1: preparation of 0.5% spinosad and aggregation pheromone compound powder
Mixing spinosad 0.5%, pest pheromone 0.002% (specifically, cervus ruber aggregated pheromone 0.0005%, rice weevil aggregated pheromone 0.0005%, Indian meal moth aggregated pheromone 0.001%), nano titanium dioxide 0.1%, and pulvis Talci to 100%, uniformly mixing the above raw materials at a certain proportion, jet-milling uniformly, and packaging.
Example 2: preparation of 5% spinosad and aggregation pheromone compound suspending agent
The spinosad and pheromone compound suspending agent consists of spinosad, pest pheromone, organic solvent and water, and the weight percentage of each component is as follows: 5% of pleocidin, 0.005% of pest pheromone (specifically, 0.001% of rusty akage agglutinin, 0.001% of rice weevil agglutinin, 0.001% of khaki agglutinin and 0.002% of sabatier agglutinin), 0.2% of xanthan gum (thickening agent), 2% of ethylene glycol (antifreezing agent) and the balance of water. The preparation method comprises the following steps.
Firstly, crushing spinosad powder by using an ultramicro crusher, then adding a raw spinosad, the coleoptera pest gathering pheromone, the thickening agent, the antifreezing agent and water into a container with a stirring paddle, stirring for 30min, uniformly mixing, and dispersing for 20min to be uniform in a high-speed shearing emulsifying machine at the speed of 5000 r/min. Then 0.05 percent of defoaming agent is added, and the mixture is dispersed for 20 minutes in a high-speed shearing emulsifying machine at the speed of 2000r/min to prepare the uniform compound suspending agent.
Example 3: preparation of 20% spinosad and aggregation pheromone compound suspending agent
The weight percentages of the components are as follows: spinosad 20%, pest aggregation pheromone 0.1% (specifically, rice weevil aggregation pheromone 0.02%, rice moth aggregation pheromone 0.02%, wheat moth aggregation pheromone 0.02% and Indian meal moth aggregation pheromone 0.04%), xanthan gum (thickener) 0.3%, ethylene glycol (antifreeze) 5% and the balance of water. The preparation method comprises the following steps.
Firstly, pleocidin powder is crushed by an ultrafine crushing instrument, then pleocidin raw pesticide, rice weevil aggregation pheromone, silverfish aggregation pheromone, wheat moth aggregation pheromone, Indian meal moth aggregation pheromone, thickening agent, antifreezing agent and water with the mixture amount are added into a container with a stirring paddle, and after stirring for 30min and uniform mixing, the mixture is dispersed in a high-speed shearing emulsifying machine for 20min to be uniform at the speed of 5000 r/min. Then 0.05 percent of silicone oil defoaming agent is added, and the mixture is dispersed for 20 minutes in a high-speed shearing emulsifying machine at the speed of 2000r/min to prepare the uniform compound suspending agent.
Effect example 1: insecticidal toxicity test
The compounded synergistic suspending agent prepared by the method of the embodiment 2 is used for determining the insecticidal toxicity, and the tested pests are corn weevil, ips typographus and tribolium castaneum and are provided by a grain storage insect feeding room of the university of light industry in Wuhan. The corn elephant is bred by whole grain wheat with the water content of (13 +/-1)%, the grain beetle is bred by half-broken wheat, and the red grain beetle is bred by whole wheat flour. The test insects are fed at the temperature of 28 +/-1 ℃ and the relative humidity of 75 +/-5 percent, and the test is carried out on the adult insects which are hatched for two weeks. The pest pheromone is purchased from Biotech limited of Hualiang, Anhui; spinosad technical material was purchased from zilu pharmaceutical (inner mongolia) ltd with a purity of about 92%.
(1) Determination of insecticidal toxicity of compound suspending agent to stored grain pests in simulation bin
The 5% spinosyn and pheromone compounded suspending agent was prepared according to the method of example 2.
Preparation of 5% spinosyn suspension: the weight percentages of the components are as follows: 5% of spinosad, 0.2% of xanthan gum (thickening agent), 2% of glycol (antifreezing agent) and the balance of water. The preparation method comprises the following steps.
Firstly, crushing spinosad powder by using an ultramicro crusher, then adding the raw spinosad, a thickening agent, an antifreezing agent and water in the mixture according to the mixture ratio into a container with a stirring paddle, stirring for 30min, uniformly mixing, and dispersing for 20min to be uniform in a high-speed shearing emulsifying machine at the speed of 5000 r/min. Then 0.05 percent of defoaming agent is added, and the mixture is dispersed for 20 minutes in a high-speed shearing emulsifying machine at the speed of 2000r/min to prepare the uniform compound suspending agent.
Mixing with grain to volume of about 0.05m3The closed cylindrical container is a simulation bin, 10 kilograms of wheat are finally loaded in each simulation bin, 10 kilograms of grains are used for pesticide application in the whole bin, and 9 kilograms of grains which are not used for pesticide application are added at the bottom of the simulation bin and 1 kilogram of grains for pesticide application is added on the simulation bin.
The experimental group respectively measures the preparation according to the dosage of each treatment and dilutes the preparation and water according to a certain proportion (100-500 times), and then sprays the medicament through a pressure sprayer to uniformly mix the medicament on the wheat. 50 adults of corn weevil, ips and tribolium castaneum (25 adults of each pest) are respectively connected to the bottom of each simulation bin, then the processed grains are separately loaded into the container simulation granaries, and 9 repetitions are set for each processing. 3 times of repeated investigation of pest death conditions are respectively taken on 3 rd, 7 th and 15 th days after the pest feeding, and the corrected mortality is calculated.
Corrected mortality-control mortality)/(1-control mortality) x 100%.
Pest death criteria: the patients with tail, horn and foot immobility should be killed by the dissecting needle.
Data were analyzed using SPSS 17.0.
TABLE 1. toxicity of Spinosad and Coleoptera pest aggregation pheromone compounded suspending agents on maize weevils
TABLE 2 Spinosad and Coleoptera pest aggregation pheromone compounded suspending agent virulence to khapra beetle
TABLE 3 toxicity of Spinosad and Coleoptera pest aggregation pheromone compounded suspending agent on tribolium castaneum
Effect example 2: insecticidal toxicity test experiment 2
The compounded synergistic suspending agent prepared by the method of example 3 is used for insecticidal toxicity determination, and the tested pests are the wheat moth and the Indian meal moth, which are provided by a grain storage insect feeding room of Wuhan university of light industry for incubation of two weeks for testing. The pest pheromone is purchased from Biotech limited of Hualiang, Anhui; spinosad technical material was purchased from zilu pharmaceutical (inner mongolia) ltd with a purity of about 92%.
(1) Determination of insecticidal toxicity of compound suspending agent to stored grain pests in simulated barn
A 20% spinosyn and aggregation pheromone compounded suspension was prepared according to the method of example 3.
Preparation of 2% spinosyn suspension: the weight percentages of the components are as follows: spinosad 20%, xanthan gum (thickener) 0.3%, glycol (antifreeze) 5% and the balance of water. The preparation method comprises the following steps.
Firstly, crushing spinosad powder by using an ultramicro crusher, then adding the raw spinosad, a thickening agent, an antifreezing agent and water in the mixture according to the mixture ratio into a container with a stirring paddle, stirring for 30min, uniformly mixing, and dispersing for 20min to be uniform in a high-speed shearing emulsifying machine at the speed of 5000 r/min. Then 0.05 percent of silicone oil defoaming agent is added, and the mixture is dispersed for 20 minutes in a high-speed shearing emulsifying machine at the speed of 2000r/min to prepare the uniform compound suspending agent.
Mixing with grain to volume of about 0.05m3The closed cylindrical container is a simulation bin, 10 kilograms of wheat are finally loaded in each simulation bin, 10 kilograms of grains are used for pesticide application in the whole bin, and 9 kilograms of grains which are not used for pesticide application are added at the bottom of the simulation bin and 1 kilogram of grains for pesticide application is added on the simulation bin.
The experimental group respectively measures the preparation according to the dosage of each treatment and dilutes the preparation and water according to a certain proportion (100-500 times), and then sprays the medicament through a pressure sprayer to uniformly mix the medicament on the wheat. 50 larvae of the two types of larvae of the wheat moth and the Indian meal moth (25 larvae of the male and female larvae) are inoculated to the bottom of each simulation bin, then the processed grains are respectively loaded into the container simulation bins, and 9 repetitions are set for each processing. 3 times of repeated investigation of pest death conditions are respectively taken on 3 rd, 7 th and 15 th days after the pest feeding, and the corrected mortality is calculated.
Corrected mortality-control mortality)/(1-control mortality) x 100%.
Pest death criteria: the patients with tail, horn and foot immobility should be killed by the dissecting needle.
Data were analyzed using SPSS 17.0.
TABLE 4 toxicity test results of spinosad and pest aggregation pheromone compounded suspending agent on wheat moth
TABLE 5 Duplexcidin and Pest aggregation pheromone compounded suspending agent toxicity test results on Indian meal moth
The results in tables 1-5 show that in the spinosad and coleoptera and/or lepidoptera pest aggregation pheromone compound suspending agent, the effective concentration of the spinosad is 1.0ppm, the control effect of the spinosad on corn weevils, silverfish, tribolium castaneum, wheat moths and Indian meal moth is obviously superior to that of the spinosad suspending agent used alone, and meanwhile, the compound agent disclosed by the invention has a quick action and obviously improves the insecticidal efficiency by simultaneously trapping and killing insects of two genders. And the total amount of the insecticide in the compound agent is low, the pesticide effect is more stable, the price is cheaper, and the cost advantage is better. In addition, the compound agent in the prior art can only trap and kill insects of a single sex or cannot achieve high insecticidal efficiency (namely, the compound agent is only effective for male pests or only female pests) for insects of two sexes at the same time, but in the effect example of the invention, the ratio of the male pests to the female pests is 1:1 (25 heads for each female and male larva), namely, the compound agent can simultaneously attract the pests of two sexes, can achieve indiscriminate trapping and killing for the insects of two sexes, and has higher insecticidal efficiency.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and such improvements and modifications should be considered as the protection scope of the present invention.
Claims (10)
1. A compound composition, wherein the compound composition comprises spinosad and pest aggregation pheromone.
2. The compounded composition according to claim 1, wherein the pest aggregation pheromone comprises a coleopteran pest aggregation pheromone and/or a lepidopteran pest aggregation pheromone;
preferably, the mass ratio of the spinosad to the pest aggregation pheromone is 10:1-200000:1, preferably 100:1-10000: 1;
preferably, the coleopteran pest aggregation pheromone is selected from one or more of a rubisco aggregation pheromone, a rice weevil aggregation pheromone, a khaki aggregation pheromone and a sabatier aggregation pheromone; the lepidoptera pest aggregation pheromone is selected from one or more of indian meal moth aggregation pheromone, wheat moth aggregation pheromone and pink rice borer aggregation pheromone.
3. A stored-grain protective agent comprising the compounded composition of claim 1 or 2 and one or more auxiliary agents;
preferably, the ratio of the spinosad in the grain storage protective agent is 0.5-20 wt%;
preferably, the proportion of the pest aggregation pheromone in the grain storage protective agent is 0.0001-0.1 wt%;
preferably, the proportion of the compound composition in the grain storage protective agent is 0.5-21 wt%.
4. The grain storage protective agent of claim 3, wherein the grain storage protective agent is in the form of powder, aqueous emulsion or suspending agent;
preferably, the auxiliary agent is one or more of a cosolvent, an emulsifier, a dispersant, a wetting agent, a thickener, an antifreeze, a defoamer or a filler.
5. The grain storage protective agent of claim 4, wherein the grain storage protective agent is a powder, wherein the powder comprises: 0.5-2 wt% of pleocidin, 0.0001-0.002 wt% of pest aggregation pheromone, 0.1-0.2 wt% of nano titanium dioxide, and the balance of talcum powder to 100 wt%.
6. The grain storage protective agent of claim 4, wherein the grain storage protective agent is a suspending agent, wherein the suspending agent comprises: 1-20 wt% of spinosad, 0.0001-0.1 wt% of pest aggregation pheromone, 0.1-3 wt% of xanthan gum, 1-10 wt% of ethylene glycol, 0.01-1 wt% of defoaming agent and the balance of water, wherein the defoaming agent is preferably a silicone oil defoaming agent.
7. A method of preparing a stored grain protective agent as claimed in any one of claims 3 to 6, which comprises compounding and mixing spinosad with a pest aggregation pheromone and one or more adjuvants, preferably the pest aggregation pheromone comprises a coleopteran pest aggregation pheromone and/or a lepidopteran pest aggregation pheromone.
8. The method of claim 7, wherein the grain-storage protective agent is a powder, and the preparation method of the powder comprises the following steps: mixing 0.5-2 wt% of pleocidin, 0.0001-0.002 wt% of pest aggregation pheromone and 0.1-0.2 wt% of nano titanium dioxide, supplementing to 100 wt% with talcum powder, and performing jet milling.
9. The method of claim 7, wherein the grain storage protective agent is a suspending agent, and the preparation method of the suspending agent comprises the following steps:
(1) mixing and stirring the crushed pleocidin, the pest aggregation pheromone, the thickening agent, the antifreezing agent and water for 20-30min, and dispersing in a high-speed shearing emulsifying machine at the speed of 5000-; and
(2) adding 0.01-1 wt% of defoaming agent, and dispersing in a high-speed shearing emulsifying machine at the speed of 1000-3000r/min for 20-30 minutes to prepare the uniform compound suspending agent.
10. Use of a built composition according to claim 1 or 2 and a grain storage protectant according to any one of claims 3 to 6 for controlling grain storage pests;
preferably, the pests include one or more of the following: tribolium castaneum, rice weevil, corn weevil, rice moth, Indian meal moth, pink spot moth;
preferably, the compound composition or the grain storage protective agent kills pests by full-warehouse application or surface application of grains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111429261.1A CN114467945A (en) | 2021-11-29 | 2021-11-29 | Grain storage protective agent containing spinosad and aggregation pheromone and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111429261.1A CN114467945A (en) | 2021-11-29 | 2021-11-29 | Grain storage protective agent containing spinosad and aggregation pheromone and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114467945A true CN114467945A (en) | 2022-05-13 |
Family
ID=81492750
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111429261.1A Pending CN114467945A (en) | 2021-11-29 | 2021-11-29 | Grain storage protective agent containing spinosad and aggregation pheromone and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114467945A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381233A (en) * | 2014-12-10 | 2015-03-04 | 甘肃惠森药业发展有限公司 | Method for preventing and controlling medicinal material storage pests by virtue of pheromone |
| CN105494444A (en) * | 2014-10-15 | 2016-04-20 | 浙江新安化工集团股份有限公司 | Insecticidal composition capable of high-precisely and efficiently controlling homoptera pests and applications thereof |
| CN105594747A (en) * | 2014-10-15 | 2016-05-25 | 浙江新安化工集团股份有限公司 | Precisely-oriented and efficient pest prevention and control insect disinfestation composition and use method thereof |
| CN105746563A (en) * | 2016-04-26 | 2016-07-13 | 国家粮食局科学研究院 | Pleocidin and athomin compounded composition and application thereof |
-
2021
- 2021-11-29 CN CN202111429261.1A patent/CN114467945A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494444A (en) * | 2014-10-15 | 2016-04-20 | 浙江新安化工集团股份有限公司 | Insecticidal composition capable of high-precisely and efficiently controlling homoptera pests and applications thereof |
| CN105594747A (en) * | 2014-10-15 | 2016-05-25 | 浙江新安化工集团股份有限公司 | Precisely-oriented and efficient pest prevention and control insect disinfestation composition and use method thereof |
| CN104381233A (en) * | 2014-12-10 | 2015-03-04 | 甘肃惠森药业发展有限公司 | Method for preventing and controlling medicinal material storage pests by virtue of pheromone |
| CN105746563A (en) * | 2016-04-26 | 2016-07-13 | 国家粮食局科学研究院 | Pleocidin and athomin compounded composition and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2012272776A1 (en) | Pesticide compositions containing thyme oil and cinnamon oil | |
| CN101637172B (en) | Insecticidal/acaricidal composition having lufenuron and tebufenozide | |
| CN101984828B (en) | Compound pesticide | |
| CN101874486A (en) | Insecticide composition containing spinosad and etofenprox | |
| CN1836522A (en) | Pyrethrin missible oil formulation using neem oil as synergistic agent | |
| CN108633916A (en) | A kind of Pesticidal combination containing beauveria bassiana and ethyl pleocidin | |
| CN101444220A (en) | Buprofezin contained pesticide composition | |
| CN105746563A (en) | Pleocidin and athomin compounded composition and application thereof | |
| CN113973825B (en) | Application of allicin in preparation of insect virus synergist and synergist | |
| CN114467945A (en) | Grain storage protective agent containing spinosad and aggregation pheromone and application thereof | |
| CN113812407B (en) | Insecticidal composition containing bromofenoxaprop-p-fluorobenzene bisamide and tebuconazole and application thereof | |
| CN114027314A (en) | Insecticidal composition containing diafenthiuron and application thereof | |
| CN107372554B (en) | Formula of novel high-efficiency cockroach killing bait | |
| CN109997876A (en) | A kind of compound biotic insecticide and its preparation method and application | |
| CN112237194A (en) | Insecticidal composition containing cyclic bromoantraniliprole | |
| CN105746523B (en) | A kind of Pesticidal combination of pyriproxyfen-containing | |
| CN112568224A (en) | Insecticidal composition containing bromofenoxaprop-p-fluorobenzene bisamide and diafenthiuron | |
| CN105519568A (en) | High-efficiency pesticide containing profenofos, flubendiamide, chlorpyrifos and biopesticide lepimectin | |
| CN110024803A (en) | A kind of Pesticidal combination and its preparation of spirotetramat | |
| CN113951272B (en) | Insecticidal composition containing bromofenoxaprop-benzene bisamide and metaflumizone and application thereof | |
| CN111387182B (en) | Preparation for preventing and treating storage pests and using method thereof | |
| CN106165690A (en) | A kind of composition pesticide containing avilamycin | |
| CN104872198A (en) | High-efficiency compound pesticide composition containing plant extract, and preparation method of composition | |
| CN111374137A (en) | Insecticidal composition containing tebuconazole and chlorfenapyr | |
| CN117617259A (en) | Insecticidal composition containing tolfenpyrad and thiocyclam and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |