CN114467603A - Method for improving oil content of kernels in mature period of oil tea fruit and application of method - Google Patents
Method for improving oil content of kernels in mature period of oil tea fruit and application of method Download PDFInfo
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Abstract
The invention discloses a method for improving the oil content of kernels in the mature period of oil-tea camellia fruits and application thereof, and particularly discloses a method for preparing ethephon into a regulator, which is uniformly sprayed on the surface of an oil-tea camellia tree in 8-9 months so as to improve the oil content of kernels in the mature period of the oil-tea camellia fruits; the regulator also comprises Tween 80, and the concentrations of the ethephon and the Tween 80 are 0.50-1.00g/L and 0.035-0.06 v%, respectively. The regulator provided by the invention can accelerate the synthesis of grease and has the effect of improving the oil content of kernels; the regulator has simple components, convenient application and less spraying times; the main component of the regulator is plant endogenous hormone, so that the regulator has no environmental pollution, no side effect and obvious effect; the regulator has wide application range for increasing oil content of oil Camellia seed kernel, has effect in promoting oil content of oil Camellia seed kernel for different oil Camellia varieties, and has no influence on growth condition of oil Camellia.
Description
Technical Field
The invention belongs to the technical field of high-yield cultivation of oil tea, and particularly relates to a method for improving the oil content of kernels in the mature period of oil tea fruits and application thereof.
Background
Camellia oleifera (Camellia oleifera Abel.), also known as: tea trees and tea oil trees, wherein the tea trees belong to the family Theaceae, and are evergreen small trees, which are the unique evergreen tree species in China and the important woody edible oil tree species in China, and have important strategic effects on the aspects of guaranteeing the grain and oil safety and ecological safety in China.
The content of unsaturated fatty acid in the tea oil is up to more than 90 percent, the content of oleic acid is about 80 percent, the tea oil also contains rare health-care components such as squalene, and the tea oil and the olive oil are recommended as the best edible vegetable oil by grain and agriculture organizations of the United nations. In recent years, the area of the camellia oleifera forest base in China is continuously enlarged; however, the average oil tea yield per mu of the oil tea in China is only 30 kilograms, 75 percent of oil tea forests in China are low-yield forests, and the problem of low yield in the oil tea production becomes a scientific problem which troubles the healthy development of the oil tea industry for a long time.
Generally, the following two ways are considered to be available for increasing the oil yield per unit area of the camellia oleifera: firstly, cultivating high-yield oil tea varieties, and improving the fruit yield per unit area; and the other method is to improve the oil content of the camellia oleifera kernels by means of cultivation management and other measures. The majority of science and technology workers for oil tea research pay attention to the cultivation of high-quality varieties and select and breed some high-yield varieties; but relatively few researches on how to effectively improve the oil content of the kernels of the existing camellia oleifera varieties in production are carried out.
Therefore, the problem of low oil content of the oil-tea camellia under natural conditions is solved by increasing the oil content of the oil-tea camellia seed kernel through an artificial regulator.
Disclosure of Invention
The invention aims to solve the problem of low oil content of camellia oleifera seeds in production, and provides a method for improving the oil content of camellia oleifera seeds in the mature period of camellia oleifera fruits and application thereof.
In order to realize the purpose, the technical scheme of the invention is as follows:
on the one hand, the invention provides the application of ethephon in improving the oil content of kernels in the mature period of oil-tea camellia fruits.
Specifically, in the technical scheme, ethephon is prepared into a regulator and evenly sprayed on the surface of the camellia oleifera in 8-9 months.
In detail, in the above technical solution, the active ingredient of the regulator further comprises a surfactant, and the surfactant is preferably tween 80.
In a preferred embodiment of the invention, in the modulator, the concentration of ethephon is 0.50-1.00g/L and the content of Tween 80 is 0.035-0.06 v%.
In a particular embodiment of the invention, the solvent of the conditioning agent is water.
In the specific embodiment of the invention, the spraying frequency is 1 time, the spraying dosage is based on the degree of dripping water of the leaves, and if it rains within 12 hours after spraying, the spraying needs to be supplemented for 1 time in time.
In detail, the spraying time is usually 10 am or 5 pm, the process can be performed by using a manual sprayer or an electric sprayer, preferably an unmanned aerial vehicle, and after the spraying, the inner and outer branches of the inner and outer tree chambers of the camellia oleifera trees are uniformly sprayed until the leaves drip.
In a specific embodiment of the invention, the oil tea trees are irrigated one week before and one week after the regulator is sprayed.
In detail, in a specific embodiment of the present invention, the variety of camellia oleifera is 'huashuo' or 'huajin'.
On the other hand, the invention also provides a regulator for improving the oil content of kernels in the mature period of the oil-tea camellia fruits, wherein the effective component of the regulator comprises ethephon.
In a preferred embodiment of the invention, the concentration of ethephon in the modulator is 0.50-1.00 g/L.
Further, in the above technical scheme, the active ingredients of the regulator consist of ethephon and a surfactant.
Preferably, in the above technical solution, the surfactant is tween 80.
In detail, in the embodiment of the present invention, in the said regulator, the concentration of ethephon is 0.50-1.00g/L, and the content of Tween 80 is 0.035-0.06 v%.
In still another aspect, the present invention also provides a process for preparing the above-mentioned regulator, comprising,
weighing ethephon and water according to a certain proportion, mixing the ethephon and water uniformly, weighing surfactant (Tween 80) according to a certain proportion, adding, and stirring uniformly.
In another aspect, the invention also provides application of the regulator or the preparation method in improving the oil content of kernels in the mature period of the camellia oleifera fruits.
Compared with the prior art, the invention has the following advantages:
(1) the invention provides a regulator capable of improving the oil content of oil-tea camellia kernels, which comprises the main components of plant endogenous hormones, has no environmental pollution and no side effect, the concentration of ethephon in the regulator is 0.5-1g/L, and ethephon in the regulator can accelerate oil synthesis, so that the regulator has the effect of improving the oil content of the kernels, the oil content can be obviously improved by using the regulator provided by the invention, a foundation is laid for improving the yield of the oil-tea camellia, and the oil content of the kernels in the mature period can be effectively improved by 6.45-10.74%;
(2) the method for improving the oil content of the oil-tea camellia kernel provided by the invention can effectively overcome the problem of low oil-tea camellia yield in the prior art, establishes a method for artificially improving the oil content of the oil-tea camellia kernel, can artificially improve the oil content of the kernel, can improve the oil content of the oil-tea camellia kernel at different fruit development periods, and has the advantages of low cost, simple operation, high success rate, and extremely high application value and economic benefit;
(3) the regulator capable of improving the oil content of the oil-tea camellia kernels provided by the invention has the advantages that the main component is plant endogenous hormone, the environment pollution is avoided, no side effect is caused, the effect is obvious, the spraying can be carried out by adopting an unmanned aerial vehicle, the cost is lower, and the operability is strong;
(4) the regulator capable of improving the oil content of the oil tea seed kernel provided by the invention has a wide application range, has the effect of promoting the oil content of the oil tea seed kernel for different oil tea varieties, and has no influence on the growth condition of oil tea trees;
(5) the regulator capable of improving the oil content of the oil-tea camellia kernel provided by the invention has a wide application range, is not limited by the size of a tree body, cultivation conditions and the like, and has a wide application prospect.
Drawings
FIG. 1 is a graph showing the dynamic changes of the synthesis of seed kernel oil and fat of Camellia oleifera after spraying a regulator containing ethephon in 9/15 days, which was observed by frozen sections in Experimental example 3 of the present invention;
FIG. 2 is a graph showing the dynamic changes of the synthesis of the kernel oil and fat of Camellia oleifera after spraying tap water in 9 months and 15 days after cryo-sectioning in Experimental example 3 of the present invention;
FIG. 3 is a photograph of "Huashuo" Camellia oleifera trees corresponding to different treatments in Experimental example 3;
FIG. 4 is a photograph of "Huajin" camellia oleifera trees corresponding to different treatments in Experimental example 5 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments.
It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the examples, the means used are conventional in the art unless otherwise specified.
The terms "comprises," "comprising," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
In addition, the technical features involved in the respective embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The raw materials used in the examples of the present invention are all commercially available products.
The embodiment of the invention provides a regulator, and a preparation method of the regulator comprises the following steps:
weighing 10g of ethephon, adding water to 10L, mixing the ethephon and the water uniformly, adding 5mL of Tween 80, and stirring uniformly to obtain the regulator with ethephon concentration of 1.0g/L and Tween 80 content of 0.05 v%.
Example 1
The embodiment of the invention provides a method for improving the oil content of oil-tea camellia seed kernels, and specifically comprises the step of spraying a regulator provided by the embodiment of the invention on oil-tea camellia trees by using a sprayer before 10 am or after 5 pm of 30 days in 8 months, wherein the spraying frequency is 1 time, the inner and outer branches of the oil-tea camellia tree trunk are uniformly sprayed until leaves drip, and the spraying needs to be supplemented for 1 time if raining occurs within 12 hours after spraying.
Example 2
The embodiment of the invention provides a method for improving the oil content of oil-tea camellia kernels, which is similar to the embodiment 1; the only difference is that the spraying time is 9 months and 15 days.
Example 3
The embodiment of the invention provides a method for improving the oil content of oil-tea camellia kernels, which is similar to the embodiment 1; the only difference is that the spraying time is 9 months and 30 days.
Example 4
The embodiment of the invention provides a method for improving the oil content of oil-tea camellia kernels, which is similar to the embodiment 1; the only difference is that the spraying time is 10 months and 15 days.
Example 5
The embodiment of the invention provides a method for improving the oil content of oil-tea camellia kernels, which is similar to the embodiment 1; the only difference is that the spraying time is 10 months and 30 days.
Comparative example 1
The embodiment of the invention provides a method for improving the oil content of oil-tea camellia kernels, which is similar to the embodiment; the only difference is that it is sprayed with tap water.
Experimental example 1 correlation between oil accumulation of oil tea seed kernel and endogenous ethylene content
In the test base of camellia oleifera in east town of shone county in Hunan, in 2020, 11-year-old camellia oleifera variety 'Huashuo' with consistent management conditions is selected as a research material, the formation of oil is sampled and analyzed in 5 development periods (8 months and 30 days, 9 months and 15 days, 9 months and 30 days, 10 months and 15 days and 10 months and 30 days) of camellia oleifera kernels respectively, the oil content and the dynamic change of the content of endogenous hormone ethylene of the camellia oleifera kernels are detected, and the results are shown in Table 1.
TABLE 1 dynamic changes in oil content and endogenous ethylene content during development of oil Camellia kernels
Note: data in the table represent mean ± sd.
Analyzing the results in table 1, it can be seen that the oil content of the camellia seed kernel is always in an ascending state in each period of development; wherein 10 months and 30 days are increased by 5.98 times compared with 8 months and 30 days.
Analyzing dynamic changes of endogenous hormone ethylene content in each period of development of the camellia oleifera kernels by using high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS); the results show that the content of endogenous ethylene has a significant ascending change in each period of kernel development (8 months and 30 days, 9 months and 15 days, 9 months and 30 days, 10 months and 15 days and 10 months and 30 days), wherein 10 months and 30 days are increased by 0.73 times compared with 8 months and 30 days; the results show that the content of endogenous ethylene in the oil-tea camellia kernels is similar to the accumulation change trend of oil, and the content of endogenous ethylene in the oil-tea camellia kernels and the accumulation change trend of oil are possibly related to each other.
Experimental example 2 Effect of spraying ethephon-containing regulator on oil content of Camellia oleifera seed at different times
1. Test materials and treatment methods
Selecting a 11-year-old camellia oleifera variety 'Huashuo' high-grafting crown-changing big tree with consistent management conditions at an oil tea tree test base of the Tuqiao county of Liuyang in Hunan, in 2020, parallelly arranging 5 groups, respectively spraying a ethephon-containing regulator at 8 months 30, 9 months 15, 9 months 30 and 10 months 15 (respectively corresponding to the time of examples 1-4 and respectively recorded as test groups 1-4), and simultaneously arranging a blank control group 1 (without any spraying treatment); spraying 10 trees in each group at 9 am, wherein the treatment mode is that a spray can is used for spraying the whole camellia oleifera trees until the leaves drip.
2. Influence of exogenous ethephon on oil content of oil-tea camellia seed kernel
The materials are taken at the mature period of the camellia oleifera fruit, the oil content of camellia oleifera kernels is measured, and the results are shown in table 2.
Comparing and analyzing the results in table 2, it can be found that the oil content of the kernels of the experimental group 1 (spraying time is 8 months and 30 days), the experimental group 2 (spraying time is 9 months and 15 days) and the experimental group 3 (spraying time is 9 months and 30 days) is 35.41%, 36.64% and 36.73%, respectively, which are increased by 2.88%, 6.45% and 6.71% respectively compared with the oil content of the camellia kernels of the blank control group 1 (34.42%); compared with the oil content (34.97%) of the oil tea seed kernel of the experimental group 4 (spraying time is 10 months and 15 days), the oil tea seed kernel has no significant change. The results show that the oil content of the oil tea seed kernels can be obviously improved by carrying out exogenous ethephon treatment for 8-month 30 days, 9-month 15 days and 9-month 30 days; wherein, the effect is relatively better after spraying in 9 months and 15 days and 9 months and 30 days.
TABLE 2 Effect of ethephon-containing regulators sprayed at different times on the oil content of 'Huashuo' Camellia oleifera kernels
Note: the data in the table represent mean values. + -. standard deviation, and different lower case letters represent significant differences at P < 0.05 between different treatments of the same index.
Experimental example 3 Effect of ethephon-containing regulator sprayed on Camellia oleifera after 9 months and 15 days
1. Test materials and treatment methods
Selecting 11-year-old camellia oleifera variety 'Huashuo' high-grafting crown changing big trees with consistent management conditions as research objects in Liuyang county soil bridge Zhengcha test base in Hunan in 2020, parallelly arranging 2 groups, and spraying a ethephon-containing regulator (corresponding to example 2) and tap water (corresponding to comparative example 1) on 9, 15 days respectively; spraying 10 trees in each group at 9 am, wherein the treatment mode is that a spray can is used for spraying the whole camellia oleifera trees until the leaves drip.
Taking materials of kernels (9 months and 30 days, 10 months and 15 days and 10 months and 30 days) of camellia oleifera fruits at different periods of development, measuring the oil content of the camellia oleifera kernels, and analyzing the influence of ethephon treatment on the camellia oleifera trees at the harvest time of the camellia oleifera fruits.
2. Influence of exogenous ethephon on oil content of oil-tea camellia seed kernel
The oil content of the camellia seed kernel was measured by taking the samples at different times (9 months and 30 days, 10 months and 15 days, and 10 months and 30 days), and the results are shown in table 3.
TABLE 3 influence of spray treatment on the oil content of kernels in different developmental stages of Camellia oleifera
Note: the data in the table represent mean values. + -. standard deviation, and different lower case letters represent significant differences at P < 0.05 between different treatments of the same index.
Comparing and analyzing the results in Table 3, it can be found that the oil contents of the kernels of Camellia oleifera after 9 months and 15 days of spraying the ethephon-containing regulator are respectively 11.13%, 24.33% and 36.64% after the time of taking materials for 9 months and 30 days, 10 months and 15 days and 10 months and 30 days, and are respectively increased by 8.80%, 35.62% and 6.45% compared with the oil contents of the kernels of Camellia oleifera after 9 months and 15 days of spraying tap water (10.23%, 17.94% and 34.42%); and the synthetic dynamic change graphs of the kernel oil and fat shown in figures 1-2 also show that the number and the size of the oil bodies sprayed with the ethephon-containing regulator are obviously higher than those sprayed with tap water. In conclusion, the kernel oil content of the camellia oleifera in different development periods can be obviously improved by spraying the regulator containing ethephon in the middle 9 th month; wherein, the oil content rate of 1 month after the treatment is increased at the highest rate, so the exogenous ethephon can be used for treating to improve the oil content rate of the oil tea, thereby improving the yield of the oil tea.
3. Influence of exogenous ethephon on Camellia oleifera
In order to clarify the effect of exogenous ethephon (spraying of ethephon-containing regulator) treatment on 'gorso' camellia oleifera trees, the tree growth status, leaf chlorophyll, carotenoid content, leaf relative conductivity and leaf photosynthetic parameter photochemical efficiency (Fv/Fm) at the camellia harvest stage of 9-month-15-day spraying of ethephon-containing regulator (corresponding to example 2) and tap water (corresponding to comparative example 1) were analyzed.
FIG. 3 is a photograph of "Huashuo" Camellia oleifera trees corresponding to different treatments; as can be seen from FIG. 3, the growth of the Camellia oleifera after spraying the ethephon-containing regulator and tap water was good and no abnormal condition was observed.
TABLE 4 Effect of spray treatment on ` Large ` leaves
Note: the data in the table represent mean values. + -. standard deviation, and different lower case letters represent significant differences at P < 0.05 between different treatments of the same index.
As shown in Table 4, the contents of chlorophyll a, chlorophyll b, chlorophyll ab and carotenoid in the leaves after the ethephon-containing regulator is sprayed are respectively 1.42mg/g, 0.39mg/g, 1.81mg/g and 0.27mg/g, and the contents are not obviously different (p is more than 0.05) compared with those after tap water is sprayed; the relative conductivity of the leaves sprayed with the ethephon-containing regulator is 40.53%, and the leaves have no significant difference (p is more than 0.05) compared with the leaves sprayed with tap water (the relative conductivity is 38.96%); the photo-and parametric photochemical efficiency (Fv/Fm) of the leaves after the regulator containing ethephon is sprayed is 0.79, and the photo-and parametric photochemical efficiency (p is more than 0.05) is not obviously different from that after tap water is sprayed (the photo-and parametric photochemical efficiency is 0.81). In conclusion, spraying the ethephon-containing regulator did not affect the growth of 'wonderful' camellia oleifera.
Experimental example 4 Effect of ethephon-containing regulator sprayed on 9 months and 30 days on oil content of 'Huashuo' oil tea seed kernel development process
1. Test materials and treatment methods
Selecting 11-year-old camellia oleifera variety 'Huashuo' high-grafting crown changing big trees with consistent management conditions as research objects in a test base of native bridge town camellia oleifera in Liuyang county of Hunan province in 2020, arranging 2 groups in parallel, and spraying a regulator containing ethephon (corresponding to example 3) in 9-month and 30-day and spraying tap water (corresponding to comparative example 1) in 9-month and 30-day respectively; spraying 10 trees in each group at 9 am by using a spraying pot until leaves drip.
Taking materials of kernels of camellia oleifera fruits at different periods of development (10 months and 15 days and 10 months and 30 days), and determining the oil content of the kernels of camellia oleifera.
2. Influence of exogenous ethephon on oil content of oil-tea camellia seed kernel
The materials were collected at different periods (10 months 15 days, 10 months 30 days) respectively, and the oil content of the camellia oleifera kernels was measured, with the results shown in table 5.
TABLE 5 influence of spray treatment on the oil content of kernels in different developmental stages of Camellia oleifera
Note: the data in the table represent mean values. + -. standard deviation, and different lower case letters represent significant differences at P < 0.05 between different treatments of the same index.
Comparing and analyzing the results in Table 5, it can be found that the oil content of the kernels of Camellia oleifera after 9 months and 30 days of spraying the regulator containing ethephon is 30.76% and 36.73% respectively, and the oil content of the kernels of Camellia oleifera after 9 months and 30 days of spraying tap water (17.94% and 34.42%) is increased by 71.46% and 6.71% respectively; the results show that the oil content of the camellia seed kernel can be rapidly improved after the regulator containing ethephon is sprayed for 30 days in 9 months, and the oil content of the camellia seed kernel is also obviously higher than that of the camellia seed kernel after tap water is sprayed in the later period, but the oil content is slowly increased. In conclusion, the oil content of the camellia oleifera can be improved by spraying the ethephon-containing regulator (exogenous ethephon) in 9-month 15-day and 9-month 30-day, and the effect of the ethephon-containing regulator is slightly good.
Experimental example 5 Effect of ethephon-containing regulator sprayed on Camellia oleifera Abel in 15/9
1. Test materials and treatment methods
Selecting 11-year-old camellia oleifera variety 'Huajin' high-grafting crown-changing big trees with consistent management conditions as research objects in a camellia oleifera test base in east town of Taoist county of Hunan, in 2020, parallelly arranging 2 groups, and spraying a regulator containing ethephon (corresponding to example 2) and tap water (corresponding to comparative example 1) on 9 months and 15 days respectively; spraying 10 trees in each group at 9 am, wherein the treatment mode is that a spray can is used for spraying the whole camellia oleifera trees until the leaves drip.
Taking materials of kernels (9 months and 30 days, 10 months and 15 days and 10 months and 30 days) of camellia oleifera fruits at different periods of development, measuring the oil content of the camellia oleifera kernels, and analyzing the influence of ethephon treatment on the camellia oleifera trees at the harvest time of the camellia oleifera fruits.
2. Influence of exogenous ethephon on oil content of oil-tea camellia seed kernel
The materials are respectively obtained at different periods (30 Yue 9, 15 Yue 10 and 30 Yue 10), the oil content of the oil tea seed kernel is measured, and the results are shown in Table 6.
Comparing and analyzing the results in table 6, it can be found that the oil content of the camellia oleifera kernels obtained after 9 months and 15 days of spraying the ethephon-containing regulator and after the time of taking the ethephon-containing regulator for 9 months and 30 days, 10 months and 15 days and 10 months and 30 days is 34.29%, 35.19% and 36.21% respectively, and is increased by 7.97%, 5.90% and 8.28% respectively relative to the oil content of the camellia oleifera kernels obtained after 9 months and 15 days of spraying tap water (31.76%, 33.23% and 33.44%); the ethephon-containing regulator is sprayed in 15 days after 9 months (exogenous ethephon treatment is carried out on the camellia oleifera), so that the kernel oil content of the camellia oleifera in different development periods can be obviously improved. Therefore, the aim of improving the oil content of the oil-tea camellia by using exogenous ethephon treatment can be fulfilled for different oil-tea camellia varieties, so that the yield of the oil-tea camellia is improved.
TABLE 6 influence of spray treatment on kernel oil content of Camellia oleifera in different development stages
Note: the data in the table represent mean values. + -. standard deviation, and different lower case letters represent significant differences at P < 0.05 between different treatments of the same index.
3. Influence of exogenous ethephon on Camellia oleifera
In order to clarify the influence of exogenous ethephon (spraying of ethephon-containing regulator) treatment on the tree body of 'huajin' camellia oleifera, the tree body growth state, leaf chlorophyll, carotenoid content, leaf relative conductivity and leaf photosynthetic parameter photochemical efficiency (Fv/Fm) of camellia oleifera at harvest time of 9 months and 15 days after spraying of ethephon-containing regulator (corresponding to example 2) and tap water (corresponding to comparative example 1) were analyzed.
FIG. 4 is a photograph of Camellia oleifera 'Huajin' corresponding to different treatments; as can be seen from fig. 4, the growth state of the camellia oleifera after spraying the ethephon-containing regulator and the tap water was good, and no abnormal condition was exhibited.
As shown in Table 7, the contents of chlorophyll a, chlorophyll b, chlorophyll ab and carotenoid in the leaves after the ethephon-containing regulator is sprayed are respectively 1.16mg/g, 0.33mg/g, 1.49mg/g and 0.23mg/g, and the contents are not significantly different (p is more than 0.05) compared with those after tap water is sprayed; the relative conductivity of the leaves after the regulator containing ethephon is sprayed is 36.45%, and the leaves have no significant difference (p is more than 0.05) compared with the leaves after tap water is sprayed (the relative conductivity is 36.84%); the photo-and parametric photochemical efficiency (Fv/Fm) of the leaves after the regulator containing ethephon is sprayed is 0.81, and the photo-and parametric photochemical efficiency is not obviously different (p is more than 0.05) compared with that after tap water is sprayed (the photo-and parametric photochemical efficiency is 0.80). In conclusion, spraying the ethephon-containing regulator did not affect the growth of 'wonderful' camellia oleifera.
TABLE 7 Effect of spray treatment on 'Huajin' leaves
Note: the data in the table represent mean values. + -. standard deviation, and different lower case letters represent significant differences at P < 0.05 between different treatments of the same index.
Experimental example 6 influence of ethephon-containing regulator sprayed on 9 months and 30 days on oil content of Camellia oleifera seed kernel in development process of Huajin
1. Test materials and treatment methods
Selecting 11-year-old camellia oleifera variety 'Huajin' high-grafting crown changing big trees with consistent management conditions as research objects in a camellia oleifera test base in east town of Taoist county in Hunan, in 2020, parallelly arranging 2 groups, and spraying a regulator containing ethephon (corresponding to example 3) in 9 months and 30 days and spraying tap water (corresponding to comparative example 1) in 9 months and 30 days respectively; spraying 10 trees in each group at 9 am, wherein the treatment mode is that a spray can is used for spraying the whole camellia oleifera trees until the leaves drip.
The mature period (10 months and 30 days) of the camellia oleifera fruit is taken, and the oil content of camellia oleifera kernels is measured.
2. Influence of exogenous ethephon on oil content of oil-tea camellia seed kernel
The detection result shows that the oil content of the camellia oleifera kernels after the regulator containing ethephon is sprayed for 9 months and 30 days and the material drawing time is 10 months and 30 days is 37.03%, and is increased by 10.74% compared with the oil content (33.44%) of the camellia oleifera kernels after tap water is sprayed for 9 months and 30 days; the result shows that compared with the method of spraying the regulator containing ethephon in 9 months and 15 days, the method has better effect of improving the oil content of the kernels in the mature period by spraying the regulator containing ethephon in 9 months and 30 days (exogenous ethephon treatment is carried out on the 'Huajin' camellia oleifera trees). Therefore, exogenous ethephon treatment preferably carried out in 30 days of 9 months can achieve the purpose of increasing the oil content of the camellia oleifera, thereby increasing the yield of the camellia oleifera.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention.
It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. Application of ethephon in improving oil content of kernel of Camellia oleifera fruit in mature period.
2. The use according to claim 1,
the ethephon is prepared into a regulator, and is uniformly sprayed on the surface of the camellia oleifera in 8-9 months.
3. The use according to claim 2,
the effective component of the regulator also comprises a surfactant, and the surfactant is preferably Tween 80;
preferably, in the regulator, the concentration of ethephon is 0.50-1.00g/L, and the content of Tween 80 is 0.035-0.06 v%.
4. Use according to claim 2 or 3,
the solvent of the regulator is water;
and/or the spraying frequency is 1 time, the spraying dosage is the degree of blade dripping, and the spraying needs to be supplemented for 1 time if raining occurs within 12 hours after spraying;
and/or respectively irrigating the camellia oleifera trees one week before and one week after the regulator is sprayed.
5. The use according to any one of claims 1 to 4,
the variety of the oil tea is 'Huashuo' or 'Huajin'.
6. A regulator for improving the oil content of kernels in the mature period of oil tea fruits is characterized in that,
the effective component of the medicine comprises ethephon;
preferably, the concentration of ethephon in the regulator is 0.50-1.00 g/L.
7. The regulator according to claim 6,
the effective components of the regulator consist of ethephon and a surfactant;
preferably, the surfactant is tween 80.
8. The regulator according to claim 7,
in the regulator, the concentration of ethephon is 0.50-1.00g/L, and the content of Tween 80 is 0.035-0.06 v%.
9. A process for the preparation of the modulators of any of claims 6 to 8,
comprises weighing ethephon and water according to a certain proportion, mixing them uniformly, weighing surfactant proportionally, adding, and stirring uniformly.
10. Use of the regulator according to any one of claims 6 to 8 or the preparation method according to claim 9 for increasing the oil content of the kernel of the camellia oleifera fruit in the mature period.
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