WO2021066754A1 - Composition of durian and mangosteen extracts and method of preparation thereof - Google Patents

Composition of durian and mangosteen extracts and method of preparation thereof Download PDF

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Publication number
WO2021066754A1
WO2021066754A1 PCT/TH2020/000045 TH2020000045W WO2021066754A1 WO 2021066754 A1 WO2021066754 A1 WO 2021066754A1 TH 2020000045 W TH2020000045 W TH 2020000045W WO 2021066754 A1 WO2021066754 A1 WO 2021066754A1
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Prior art keywords
durian
extract
mangosteen
leaves
extracts
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PCT/TH2020/000045
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French (fr)
Inventor
Nopparuj TANAPATTARACHAITAT
Rujipart TANAPATTARACHAITAT
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Tipsurat Co., Ltd.
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Priority claimed from TH1903002580U external-priority patent/TH18915C3/en
Application filed by Tipsurat Co., Ltd. filed Critical Tipsurat Co., Ltd.
Publication of WO2021066754A1 publication Critical patent/WO2021066754A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

Definitions

  • This invention relates to chemistry, in particularly related to the composition of durian and mangosteen extracts and method of preparation thereof.
  • Durian is common name and a scientific name is Durio zibethinus L. (synonym scientific names are Durio acuminatissimus Merr., Durio stercoraceus Noronha) of Family Bombaceae, mostly grown in eastern and southern region of Thailand.
  • Durian is a perennial plant and is not deciduous.
  • Durian trees are growing to 8-12 metres in height.
  • Durian is classified into dicotyledons with simple leaves type. The leaves are 5-8 cm in width and 15-20 cm in length. The top of the leaves is dark green and the beneath is brown.
  • the durian flowers are perfect flower with a bell-shape and are grown in bunches.
  • Durian fruit has thick rind and sharp thorns on the surface. The texture of the durian fruit is light yellow, depending on the condition of the soil or variety.
  • Durian is a tropical fruit and widely known as "The King of Fruits" which is very popular around the world. Thailand is one of the most important planting areas for durian and is the top of the producer of durian of the world. In this regard, the waste of durian rinds was increased. There are several methods to get rid of the durian rinds by using them as fertilizer or fuel. In addition to that, there are also the studies to seek on how to use the durian rind in more useful way. The study found that the durian rind is rich in dietary fiber and the extract from the durian rind is also beneficial to the human body in many ways.
  • Mangosteen is widely known as "The Queen of Fruits". According to the scientific studies, it was found that an extract from the mangosteen peel has antioxidant activity and medical properties. There have been use of the extract of mangosteen peel for medical and cosmetics purposes. In 2011, Li Wang and Xican Li studied the antioxidant activity of durian rind in vitro using DPPH, ABTS, FRAP and total phenolic content method. It was found that the extract of durian rind extracted with methanol had antioxidant activity in vitro. This makes Durian rind a natural source of antioxidant and may have medical benefit (Wang, L., Li, XC 2011. Antioxidant activity of Durian (Durio zibethinus Murr.) Shell in vitro. Asian J. of Pharmaceut. Biol.Res., 1, 542-551.)
  • Dr. Shela Gorinstein presented the study of antioxidant content found in the durian by conducting two-factor studies including durian varieties and the stage of ripening. The study found that Monthong durian had higher antioxidant activity than Chanee, Kanyao, Puangmanee and Kradum Durian.
  • durian rinds are large, and having sharp thorns surface. It is also difficult to transport. It is required to proceed with the process of handling the durian rind into small pieces in order to extract.
  • the disadvantage is that it is a time consuming process. Raw material source management is not stable so that it is unable to control the quality of the raw material used for extraction for further commercial use.
  • Durian flowers often blossom in large quantities by using synthetic nutrient to raise flowers.
  • the growers In order to control the good condition of growth of durian fruits, the growers have to cut and trim flowers continuously in order to balance the nutrient to raise fruits.
  • the durian tops, durian flowers and durian young leaves are the waste parts of the durian trees.
  • the inventor then created the concept to using durian tops, durian flowers, and durian leaves in extraction to study antioxidant activity for further use in particular for cosmetics purpose. According to the search result, it was not found that there is the extraction of durian tops, durian flowers and durian leaves by using propylene glycol as a solvent. In addition, it is also not found that there is the mix between durian extracts and mangosteen extracts for using as an antioxidant active ingredient in cosmetics. Therefore, this invention was developed by using the durian tops, durian flowers and durian leaves were tested for analysis of antioxidant.
  • this invention was developed to decrease such disadvantages and limitations by inventing the extracts of the durian tops, durian flowers and durian leaves and the combination of the said extracts mixed with the extract of mangosteen peel that having high antioxidant activity to be utilized in various fields, especially for cosmetics purpose.
  • the object of the present invention is to provide a composition of durian and mangosteen extracts, characterized in that the composition comprising an extract selected from an extract of durian tops or an extract of durian flowers or an extract of durian leaves or a combination of at least two kinds of said durian extracts mixed with an extract of mangosteen peel in the ratio of 1 : 1 by volume.
  • a method of preparation of the composition of durian and mangosteen extracts comprising preparing the extract of mangosteen peel; preparing the extract of durian tops or the extract of durian flowers or the extract of durian leaves in which the solvent is propylene glycol; mixing the extracts of durian tops or the extract of durian flowers or the extract of durian leaves or the combination of at least two kinds of said durian extracts with the extracts of mangosteen peel in the ratio of 1 : 1 by volume.
  • this invention also provided the extract of durian tops or durian flowers or durian leaves by using propylene glycol as the solvent.
  • Another object of this invention is to invent the extract of durian and mangosteen, in particular to those selected from the extract of durian tops or durian flowers or durian leaves or the combination of at least two kinds of said durian extracts and mixing with the extract of mangosteen peel in order for use as an antioxidant in cosmetics. Further advantages of the present invention will be apparent in view of the detailed description below.
  • the invention relates to a composition of durian and mangosteen extracts, characterized in that the composition comprising an extract selected from an extract of durian tops or an extract of durian flowers or an extract of durian leaves or a combination of at least two kinds of said durian extracts mixed with an extract of mangosteen peel in the ratio of 1 : 1 by volume.
  • a method of preparation of the composition of durian and mangosteen extracts comprising the following steps:
  • the steps start from washing mangosteen peel and making into small pieces and then immersing in the solvent for extraction process.
  • the solvent is 95% ethanol.
  • the ratio of mangosteen peel to the solvent is 1 : 2 by weight per volume.
  • shaking every 8 hours and storing at room temperature for 3 days. Filtering the extracted solution with filter paper.
  • the extracted extract was repeated for extraction for 2 to 3 times and is combining together.
  • the extract of mangosteen is kept at the temperature of 4°C.
  • the solvent is propylene glycol in the ratio of amount of durian tops or durian flower or durian leaves to solvent is 1 : 3 by weight per volume or equivalent to 0.03 % weight by volume in a closed container and shaking every 8 hours and storing at room temperature for 3 days.
  • the solvent used to extract durian tops or durian flowers or durian leaves is propylene glycol which is an organic glycol compound that is colorless, transparent, slightly viscous, and easily soluble in water and chloroform.
  • 1,3-propandiol also known as Zemea Propandiol, which is propylene glycol made from com can also be used as the solvent.
  • durian is Monthong variety having antioxidant activity tested by DPPH method at 96.08%.
  • mangosteen The suitable variety of mangosteen is Mangosteen from Rayongzhou, Thailand.
  • the composition of durian and mangosteen extracts comprises the extracts of durian tops or the extract of durian flowers or the extract of durian leaves or the combination of at least two kinds of said durian extracts using propylene glycol as the solvent with the concentration of 0.03% weight by volume, mixed with the extract of mangosteen peel with the concentration of 1 mg/ml in a ratio of 1 : 1 by volume.
  • the antioxidant activity test of the extract was carried out by using DPPH, ABTS method, and the determination of phenolic content by Folin ciocalteu reagent method by using extract with concentration of 1 mg/ml compared to the gallic acid equivalent at the concentration in the range of 0-200 pg/ml. The results were reported in mgGE/g. The results are shown in Table 1. Table 1 Examples of the testing results of antioxidant activity of the extracts.
  • Table 1 shows the percentage of Trolox equivalent antioxidant capacity of the extract of durian tops extracted by propylene glycol and Zemea propandiol by DPPH and ABTS method.
  • the result shows that when mixing Rayong Mangosteen peel extract with the concentration of 1 mg/ml with the extract of durian tops extracted by propylene glycol with the concentration of 0.03 % in the ratio of 1: 1, the antioxidant activity is higher than other extracts in which the percentage of Trolox equivalent antioxidant capacity by DPPH and ABTS method is 22.52 ⁇ 0.03 and 71.05 ⁇ 0.01 mg TEAC/g extract, respectively.
  • the Phenolic content is 235.38 ⁇ 0.05 (mgGE/g).
  • the method of preparation of the extract of durian tops or durian flowers or durian leaves can perform by the process described above but using 95% ethanol as the solvent instead. However, it takes longer time to extract than using propylene glycol or Zemea propandiol.
  • this invention can also comprise only the extract of durian, which consists of durian tops or durian flowers or durian leaves using propylene glycol or Zemea propandiol as the solvent for use as an antioxidant in cosmetics.
  • the method of extracting the extract from durian are as follows:
  • the solvent is propylene glycol in the ratio of amount of durian tops or durian flower or durian leaves to solvent is 1: 3 by weight per volume or equivalent to 0.03 percent weight by volume in a closed container and shaking every 8 hours and storing at room temperature for 3 days.

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Abstract

A composition of durian and mangosteen extracts comprising an extract selected from an extract of durian tops or an extract of durian flowers or an extract of durian leaves or a combination of at least two kinds of said durian extracts, mixed with an extract of mangosteen peel in a ratio of 1: 1 by volume wherein the solvent for extract the extract of the durian tops or the extract of durian flowers or the extracts of durian leaves is propylene glycol.

Description

TITLE OF THE INVENTION
COMPOSITION OF DURIAN AND MANGOSTEEN EXTRACTS AND METHOD
OF PREPARATION THEREOF.
TECHNICAL FIELD
This invention relates to chemistry, in particularly related to the composition of durian and mangosteen extracts and method of preparation thereof.
BACKGROUND ART
Durian is common name and a scientific name is Durio zibethinus L. (synonym scientific names are Durio acuminatissimus Merr., Durio stercoraceus Noronha) of Family Bombaceae, mostly grown in eastern and southern region of Thailand. Durian is a perennial plant and is not deciduous. Durian trees are growing to 8-12 metres in height. Durian is classified into dicotyledons with simple leaves type. The leaves are 5-8 cm in width and 15-20 cm in length. The top of the leaves is dark green and the beneath is brown. The durian flowers are perfect flower with a bell-shape and are grown in bunches. Durian fruit has thick rind and sharp thorns on the surface. The texture of the durian fruit is light yellow, depending on the condition of the soil or variety.
Durian is a tropical fruit and widely known as "The King of Fruits" which is very popular around the world. Thailand is one of the most important planting areas for durian and is the top of the producer of durian of the world. In this regard, the waste of durian rinds was increased. There are several methods to get rid of the durian rinds by using them as fertilizer or fuel. In addition to that, there are also the studies to seek on how to use the durian rind in more useful way. The study found that the durian rind is rich in dietary fiber and the extract from the durian rind is also beneficial to the human body in many ways.
Mangosteen is widely known as "The Queen of Fruits". According to the scientific studies, it was found that an extract from the mangosteen peel has antioxidant activity and medical properties. There have been use of the extract of mangosteen peel for medical and cosmetics purposes. In 2011, Li Wang and Xican Li studied the antioxidant activity of durian rind in vitro using DPPH, ABTS, FRAP and total phenolic content method. It was found that the extract of durian rind extracted with methanol had antioxidant activity in vitro. This makes Durian rind a natural source of antioxidant and may have medical benefit (Wang, L., Li, XC 2011. Antioxidant activity of Durian (Durio zibethinus Murr.) Shell in vitro. Asian J. of Pharmaceut. Biol.Res., 1, 542-551.)
In 2018, Jiarying Feng et.al, studied triterpenoid and glycosides from durian rind extract regarding the anti-inflammatory effect by studying chromatography method and biological activity testing. Durian rind was found to be anti-inflammatory (J. Feng, X. Yi, W. Huang, Y. Wang & X. He. 2018. Novel triterpenoids and glycosides from durian exert pronounced anti-inflammatory activities. Food Chemistry, 241, 215-211.)
In 2018, Dr. Shela Gorinstein presented the study of antioxidant content found in the durian by conducting two-factor studies including durian varieties and the stage of ripening. The study found that Monthong durian had higher antioxidant activity than Chanee, Kanyao, Puangmanee and Kradum Durian.
The problems encountered from the utilization of durian rind in extraction for the extracts are that the durian rinds are large, and having sharp thorns surface. It is also difficult to transport. It is required to proceed with the process of handling the durian rind into small pieces in order to extract. The disadvantage is that it is a time consuming process. Raw material source management is not stable so that it is unable to control the quality of the raw material used for extraction for further commercial use.
Durian flowers often blossom in large quantities by using synthetic nutrient to raise flowers. In order to control the good condition of growth of durian fruits, the growers have to cut and trim flowers continuously in order to balance the nutrient to raise fruits. As well as for the durian tops and young leaves that need to be trimmed in order for great condition for growth of the fruits. Therefore, the durian tops, durian flowers and durian young leaves are the waste parts of the durian trees. The inventor then created the concept to using durian tops, durian flowers, and durian leaves in extraction to study antioxidant activity for further use in particular for cosmetics purpose. According to the search result, it was not found that there is the extraction of durian tops, durian flowers and durian leaves by using propylene glycol as a solvent. In addition, it is also not found that there is the mix between durian extracts and mangosteen extracts for using as an antioxidant active ingredient in cosmetics. Therefore, this invention was developed by using the durian tops, durian flowers and durian leaves were tested for analysis of antioxidant.
Therefore, this invention was developed to decrease such disadvantages and limitations by inventing the extracts of the durian tops, durian flowers and durian leaves and the combination of the said extracts mixed with the extract of mangosteen peel that having high antioxidant activity to be utilized in various fields, especially for cosmetics purpose.
SUMMARY OF THE INVENTION
The object of the present invention is to provide a composition of durian and mangosteen extracts, characterized in that the composition comprising an extract selected from an extract of durian tops or an extract of durian flowers or an extract of durian leaves or a combination of at least two kinds of said durian extracts mixed with an extract of mangosteen peel in the ratio of 1 : 1 by volume.
A method of preparation of the composition of durian and mangosteen extracts comprising preparing the extract of mangosteen peel; preparing the extract of durian tops or the extract of durian flowers or the extract of durian leaves in which the solvent is propylene glycol; mixing the extracts of durian tops or the extract of durian flowers or the extract of durian leaves or the combination of at least two kinds of said durian extracts with the extracts of mangosteen peel in the ratio of 1 : 1 by volume.
In addition, this invention also provided the extract of durian tops or durian flowers or durian leaves by using propylene glycol as the solvent.
Another object of this invention is to invent the extract of durian and mangosteen, in particular to those selected from the extract of durian tops or durian flowers or durian leaves or the combination of at least two kinds of said durian extracts and mixing with the extract of mangosteen peel in order for use as an antioxidant in cosmetics. Further advantages of the present invention will be apparent in view of the detailed description below.
DETAILED DESCRIPTION OF THE INVENTION
The invention relates to a composition of durian and mangosteen extracts, characterized in that the composition comprising an extract selected from an extract of durian tops or an extract of durian flowers or an extract of durian leaves or a combination of at least two kinds of said durian extracts mixed with an extract of mangosteen peel in the ratio of 1 : 1 by volume.
A method of preparation of the composition of durian and mangosteen extracts comprising the following steps:
1. Preparing the extract of mangosteen peel.
The steps start from washing mangosteen peel and making into small pieces and then immersing in the solvent for extraction process. The solvent is 95% ethanol. The ratio of mangosteen peel to the solvent is 1 : 2 by weight per volume. Then, shaking every 8 hours and storing at room temperature for 3 days. Filtering the extracted solution with filter paper. The extracted extract was repeated for extraction for 2 to 3 times and is combining together. Then, removing solvent by using Rotary Evaporator in order to get the extract of mangosteen. Then, the extract of mangosteen is kept at the temperature of 4°C.
Characterized in that the method of preparation further comprising the following steps:
2. Preparing the extract of durian tops or the extract of durian flowers or the extracts of durian leaves by
- Washing the durian tops or durian flowers or durian leaves.
- Immersing in solvent for extraction. The solvent is propylene glycol in the ratio of amount of durian tops or durian flower or durian leaves to solvent is 1 : 3 by weight per volume or equivalent to 0.03 % weight by volume in a closed container and shaking every 8 hours and storing at room temperature for 3 days.
- Filtering the extracted solution with filter paper. - Storing the extract of the durian top or durian flowers or durian leaves at a temperature of 4°C.
3. Mixing the extract of durian tops or durian flowers or durian leaves or the combination of at least two kinds of said durian extracts with the extract of mangosteen peel in the ratio of 1 : 1 by volume.
Preferably, the solvent used to extract durian tops or durian flowers or durian leaves is propylene glycol which is an organic glycol compound that is colorless, transparent, slightly viscous, and easily soluble in water and chloroform. Alternatively, 1,3-propandiol, also known as Zemea Propandiol, which is propylene glycol made from com can also be used as the solvent.
The most suitable variety of durian is Monthong variety having antioxidant activity tested by DPPH method at 96.08%.
The suitable variety of mangosteen is Mangosteen from Rayong Province, Thailand.
Preferably, the composition of durian and mangosteen extracts comprises the extracts of durian tops or the extract of durian flowers or the extract of durian leaves or the combination of at least two kinds of said durian extracts using propylene glycol as the solvent with the concentration of 0.03% weight by volume, mixed with the extract of mangosteen peel with the concentration of 1 mg/ml in a ratio of 1 : 1 by volume.
Example of the testing results of antioxidant activity of the extract.
The antioxidant activity test of the extract was carried out by using DPPH, ABTS method, and the determination of phenolic content by Folin ciocalteu reagent method by using extract with concentration of 1 mg/ml compared to the gallic acid equivalent at the concentration in the range of 0-200 pg/ml. The results were reported in mgGE/g. The results are shown in Table 1. Table 1 Examples of the testing results of antioxidant activity of the extracts.
Figure imgf000007_0001
Table 1 shows the percentage of Trolox equivalent antioxidant capacity of the extract of durian tops extracted by propylene glycol and Zemea propandiol by DPPH and ABTS method. The result shows that when mixing Rayong Mangosteen peel extract with the concentration of 1 mg/ml with the extract of durian tops extracted by propylene glycol with the concentration of 0.03 % in the ratio of 1: 1, the antioxidant activity is higher than other extracts in which the percentage of Trolox equivalent antioxidant capacity by DPPH and ABTS method is 22.52 ± 0.03 and 71.05 ± 0.01 mg TEAC/g extract, respectively. Also, the Phenolic content (gallic acid equivalent) is 235.38 ± 0.05 (mgGE/g).
Another embodiment, the method of preparation of the extract of durian tops or durian flowers or durian leaves can perform by the process described above but using 95% ethanol as the solvent instead. However, it takes longer time to extract than using propylene glycol or Zemea propandiol.
Another embodiment, this invention can also comprise only the extract of durian, which consists of durian tops or durian flowers or durian leaves using propylene glycol or Zemea propandiol as the solvent for use as an antioxidant in cosmetics. The method of extracting the extract from durian are as follows:
- Washing the durian tops or durian flower or durian leaves. - Immersing in the solvent for extraction. The solvent is propylene glycol in the ratio of amount of durian tops or durian flower or durian leaves to solvent is 1: 3 by weight per volume or equivalent to 0.03 percent weight by volume in a closed container and shaking every 8 hours and storing at room temperature for 3 days.
- Filtering the extracted solution with filter paper.
- Storing the extract of durian tops or durian flowers or durian leaves at a temperature of 4°C.

Claims

1. A composition of durian and mangosteen extracts, characterized in that the composition comprising an extract selected from an extract of durian tops or an extract of durian flowers or an extract of durian leaves or a combination of at least two kinds of said durian extracts, mixed with an extract of mangosteen peel in a ratio of 1 : 1 by volume.
2. The composition of durian and mangosteen extracts according to claim 1, wherein the extract of the durian tops or the extract of durian flowers or the extracts of durian leaves are extracted by using propylene glycol as a solvent.
3. The composition of durian and mangosteen extracts according to claim 1, wherein the extract of the mangosteen peel has concentration of 1 mg/ml and the extract of durian tops or the extract of durian flowers or the extract of durian leaves or the combination of at least two kinds of said durian extracts, extracted by using propylene glycol with concentration of 0.03 percent weight by volume as the solvent.
4. The composition of durian and mangosteen extracts according to claim 1, wherein the extract of durian tops or the extract of durian flowers or the extract of durian leaves is extracted by using Zemea propandiol as the solvent.
5. The composition of durian and mangosteen extracts according to claim 1, wherein the extract of the mangosteen peel is extracted by using 95% ethanol as the solvent.
6. The composition of durian and mangosteen extracts according to claim 1, wherein the durian is Monthong variety.
7. A method of preparation of the composition of durian and mangosteen extracts comprising the following steps:
- Preparing the extract of mangosteen peel comprising: washing mangosteen peel and making into small pieces and then immersing in the solvent for extraction process wherein the solvent is 95% ethanol with the ratio of mangosteen peel to the solvent of 1 : 2 by weight per volume; shaking every 8 hours and storing at room temperature for 3 days; filtering the extracted solution with filter paper and the extracted extract was repeated for extraction for 2 to 3 times; combining the extracted solution; removing solvent by using Rotary Evaporator in order to get the extract of mangosteen; and keeping the extract of mangosteen at the temperature of 4°C.
Characterized in that the method of preparation further comprising the following steps:
- Preparing the extract of durian tops or the extract of durian flowers or the extracts of durian leaves
- Washing the durian tops or durian flowers or durian leaves;
- Immersing in solvent for extraction wherein the solvent is propylene glycol in the ratio of amount of durian tops or durian flower or durian leaves to solvent is 1 : 3 by weight per volume or equivalent to 0.03 % weight by volume in a closed container and shaking every 8 hours and storing at room temperature for 3 days;
- Filtering the extracted solution with filter paper;
- Storing the extract of the durian top or durian flowers or durian leaves at a temperature of 4°C;
- Mixing the extract of durian tops or durian flowers or durian leaves or the combination of at least two kinds of said durian extracts with the extract of mangosteen peel in the ratio of 1 : 1 by volume.
8. The method of preparation of the composition of durian and mangosteen extracts according to claim 7, wherein the solvent for preparation of the extract of durian tops or the extract of durian flowers or the extracts of durian leaves is Zemea propendiol.
9. An extract of durian comprising durian tops or durian flowers or durian leaves wherein the solvents for extraction is propylene glycol or Zemea propandiol.
10. A Method of manufacturing of durian extracts, characterized in that the method comprising the following steps:
- Washing the durian tops or durian flower or durian leaves;
- Immersing in solvents for extraction and the solvent is propylene glycol in the ratio of amount of durian tops or durian flower Or durian leaves to solvent is 1 : 3 by weight per volume in a closed container and shaking every 8 hours and storing at room temperature for 3 days;
- Filtering the extracted solution with filter paper;
- Storing the extract of durian top or durian flowers or durian leaves at a temperature of 4°C.
PCT/TH2020/000045 2019-10-04 2020-07-08 Composition of durian and mangosteen extracts and method of preparation thereof WO2021066754A1 (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090149418A1 (en) * 2007-12-07 2009-06-11 Sunanta Pongsamart Antimicrobial preparations using polysaccharide gel from durian fruit-rind
US20180200318A1 (en) * 2015-07-24 2018-07-19 Xantho Biotechnology Co., Ltd Use of mangosteen rind extract in preparation of medicine for treating skin diseases

Patent Citations (2)

* Cited by examiner, † Cited by third party
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