CN114451487A - Eucommia ulmoides egg and production method thereof - Google Patents

Eucommia ulmoides egg and production method thereof Download PDF

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CN114451487A
CN114451487A CN202210094685.5A CN202210094685A CN114451487A CN 114451487 A CN114451487 A CN 114451487A CN 202210094685 A CN202210094685 A CN 202210094685A CN 114451487 A CN114451487 A CN 114451487A
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eucommia ulmoides
leaf powder
eucommia
ulmoides leaf
fermented
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王俊丽
马建鑫
杜嘉琳
陆旭
耿兆鹏
袁园园
刘悦
曹爱智
尹薪项
王淑文
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Xingtai Hongmantian Family Farm LP
Minzu University of China
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Minzu University of China
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
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    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry

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Abstract

The invention discloses an eucommia ulmoides egg and a production method thereof. The production method of the eucommia ulmoides egg provided by the invention comprises the following steps: feeding laying hens to 70-75 days old by adopting a laying hen daily ration, and then feeding by adopting a laying hen feed to obtain eucommia ulmoides eggs; the layer feed comprises layer daily ration and additives, the additives are eucommia ulmoides leaf powder, fermented eucommia ulmoides leaf powder and/or eucommia ulmoides leaf extract, and the dosage of the additives is as follows: the amount of the eucommia ulmoides leaf powder is 1 to 3.5 percent of the daily ration of the laying hens; the amount of the fermented eucommia leaf powder is 2-5 per mill of the daily ration of the laying hen; the amount of the eucommia ulmoides leaf extract is 1-2 per mill of the daily ration of the laying hens. Compared with the common eggs, the eucommia ulmoides eggs produced by the laying hens fed by the eucommia ulmoides feed have the advantages that the content of DHA and EPA glycerophospholipids is increased, the B vitamins are obviously increased, and the content of histidine is obviously increased. The eucommia ulmoides egg is rich in nutrition, safe, high-quality, suitable for being eaten by many people such as hyperlipidemia and the like, and particularly suitable for being eaten by children.

Description

Eucommia ulmoides egg and production method thereof
Technical Field
The invention relates to an eucommia ulmoides egg and a production method thereof, and belongs to the technical field of eucommia ulmoides egg production.
Background
Eucommia ulmoides (Eucommia ulmoides Oliv.) is a plant of genus Eucommiaceae (Eucomiaceae) Eucommia, is a residual wiggery plant in the geological history of China and is a secondary protective wild plant in China. Eucommia bark is listed as the top grade as early as Shen nong Ben Cao Jing. The record of the pharmacopoeia of the people's republic of China: cortex Eucommiae has effects of regulating blood pressure, nourishing liver and kidney, strengthening tendons and bones, and preventing miscarriage, and can be used for treating liver and kidney deficiency, soreness of waist and knees, weakness of bones and muscles, dizziness, pregnant hemorrhage, and threatened abortion. Eucommia bark is 'treasure' in the whole body, is not only an important national strategic resource in China, but also a unique and rare medicinal material in China, and has an important position in the industries of ecological protection, medical care, green cultivation and the like.
Eucommia bark is traditionally used as the drug. Recent studies have demonstrated that: the chemical components of eucommia ulmoides leaves are similar to those of bark, and the eucommia ulmoides leaves have similar pharmacological action with the bark, namely the leaves and the bark have the same effect. At present, eucommia ulmoides leaves are already recorded in pharmacopoeia of the people's republic of China and feed raw material catalogues, and are newly published substances which are used as medicines and food. The eucommia ulmoides leaves contain various nutrient substances such as crude protein, crude fat, vitamins, amino acid and the like, can be used for feeding chickens, cattle, sheep, pigs and the like, can meet most of nutritional requirements required by the growth and development of animals, improve the immunity of livestock and poultry, reduce the occurrence of diseases and the use of antibiotics, and can greatly improve the quality of meat and eggs of the livestock and poultry, and is safe and high-quality. The application of the eucommia ulmoides leaves as the laying hen feed additive is necessary to be researched.
Disclosure of Invention
The eucommia ulmoides egg provided by the invention is rich in nutrition, safe and high-quality, is suitable for being eaten by many people such as hyperlipidemia and the like, and is particularly suitable for being eaten by children.
The invention firstly provides a preparation method of fermented eucommia leaf powder, which comprises the following steps:
s1, crushing the eucommia leaves to obtain eucommia leaf powder, and then steaming;
s2, adding Aspergillus niger into the eucommia ulmoides leaf powder obtained in the step S1, and uniformly mixing; then adding the aqueous solution of bacillus subtilis and aspergillus oryzae to obtain a mixed material; carrying out aerobic fermentation on the mixed material;
s3, inoculating enterococcus faecalis into the aerobically fermented material, continuing to ferment, and drying the fermented material to obtain the enterococcus faecalis.
In the above preparation method, in step S1, the folium cortex eucommiae powder is sieved by a 40-mesh sieve;
the steaming conditions are as follows: the temperature is 60-80 ℃, and the time is 20-30 min;
in step S2, the inoculation amount of Aspergillus niger is 3% -8%;
the inoculation amount of the bacillus subtilis is 0.1-0.5%, preferably 0.3%, and the inoculation amount of the aspergillus oryzae is 0.1-0.5%, preferably 0.3%;
the addition amount of water is 40-50% of the dry weight of the eucommia ulmoides leaf powder.
In the above preparation method, in step S2, the aerobic fermentation conditions are as follows:
turning the pile once every day at the temperature of 25-30 ℃, controlling the relative humidity of the fermentation chamber to be 70-80%, and fermenting for 5-7 d;
in the step S3, the inoculation amount of the enterococcus faecalis is 1-5%, preferably 1%;
the fermentation conditions are as follows: the temperature is 25-30 ℃, and the time is 24-36 h;
the drying conditions are as follows: drying at 40-45 ℃ until the water content of the material reaches 5% -10%.
The fermented eucommia leaf powder prepared by the method also belongs to the protection scope of the invention.
The application of the eucommia ulmoides leaf powder, the fermented eucommia ulmoides leaf powder and the eucommia ulmoides leaf extract in serving as an additive of the laying hen feed also belongs to the protection range of the invention;
the eucommia ulmoides leaf extract is prepared by the following method: soaking folium Eucommiae in water, reflux extracting, concentrating the obtained extractive solution, and drying;
soaking the raw materials in water at 70-80 ℃, repeatedly performing reflux extraction for many times, and then combining the extracting solutions;
the eucommia ulmoides leaf powder is sieved by a 40-mesh sieve.
The additive amount of the laying hen feed is as follows:
the amount of the eucommia leaf powder is 1 to 3.5 percent of the daily ration of the laying hen;
the amount of the fermented eucommia ulmoides leaf powder is 2-5 per mill of the daily ration of the laying hens;
the amount of the eucommia ulmoides leaf extract is 1-2 per mill of the daily ration of the laying hens.
The invention further provides a layer feed additive which is eucommia ulmoides leaf powder, the fermented eucommia ulmoides leaf powder and/or an eucommia ulmoides leaf extract; the mass ratio of the eucommia ulmoides leaf powder to the fermented eucommia ulmoides leaf powder to the eucommia ulmoides leaf extract is 10-35: 2-5: 1, preferably 10: 2: 1.
the invention further provides a layer feed, which comprises layer daily ration and an additive, wherein the additive is eucommia ulmoides leaf powder, fermented eucommia ulmoides leaf powder and/or eucommia ulmoides leaf extract;
the amounts of the additives were as follows:
the amount of the eucommia ulmoides leaf powder is 1 to 3.5 percent of the daily ration of the laying hen;
the amount of the fermented eucommia ulmoides leaf powder is 2-5 per mill of the daily ration of the laying hens;
the amount of the eucommia ulmoides leaf extract is 1-2 per mill of the daily ration of the laying hens.
On the basis of the laying hen feed, the invention also provides a production method of eucommia ulmoides eggs, which comprises the following steps:
the eucommia ulmoides eggs can be obtained by feeding laying hens to 70-75 days old by adopting a laying hen daily ration and then feeding the laying hens by adopting the laying hen feed.
The additive provided by the invention has obvious influence on the endogenous metabolites of the eggs. Compared with common eggs, the eucommia ulmoides eggs produced by laying hens fed with the eucommia ulmoides feed have the advantages that B vitamins are obviously increased, and glycerophospholipids containing DHA and EPA are increased, particularly PC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)/18:1(11Z)), LysoPC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)) in egg white and PC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)/18:1(11Z)) in egg yolk are increased remarkably, and PC (20:5(5Z,8Z,11Z,14Z,17Z)/P-18:1(11Z)) in egg yolk are reduced. The total amount of protein and amino acid, vitamin A, selenium, calcium, zinc and iron in the eucommia ulmoides eggs are all obviously increased, the content of 7 essential amino acids is all increased, and the content of histidine is obviously increased. Therefore, the eucommia ulmoides eggs are rich in nutrition, safe and high in quality, are suitable for being eaten by multiple people such as hyperlipidemia and are particularly suitable for being eaten by children.
Drawings
FIG. 1 is a total ion flow diagram of eucommia ulmoides egg white.
Fig. 2 is a general egg white ion flow diagram.
Fig. 3 is a total ion flow diagram of eucommia ulmoides yolk.
Fig. 4 is a general yolk total ion flow diagram.
FIG. 5 shows PCA of egg white, wherein A represents eucommia ulmoides egg white and B represents normal egg white.
FIG. 6 shows PCA of egg yolk, wherein A represents eucommia ulmoides egg yolk and B represents normal egg yolk.
FIG. 7 shows OPLS-DA of egg white, wherein A represents eucommia ulmoides egg white and B represents normal egg white.
FIG. 8 shows OPLS-DA of egg yolk, wherein A represents eucommia ulmoides egg yolk and B represents normal egg yolk.
FIG. 9 shows the color comparison of eucommia ulmoides oliv and common olives.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 preparation of folium Eucommiae feed
1. Preparation of folium Eucommiae powder
Drying the eucommia leaves: picking fresh folium Eucommiae, removing impurities, and air drying in sunlight greenhouse.
Crushing: pulverizing folium Eucommiae into 40 mesh fine powder with a pulverizer.
2. Preparation method of fermented folium Eucommiae powder
Preparing raw materials: selecting the crushed eucommia leaf powder for later use.
Steaming: steaming folium Eucommiae powder by steam method. The steam temperature is about 60 deg.C, and the steaming time is 25 min.
Inoculation: and (4) inoculating when the eucommia ulmoides leaf powder is cooled to 38-40 ℃. Aspergillus niger (the inoculum size is 5 percent and purchased from China Industrial microorganism culture preservation management center) is added into eucommia leaf powder and uniformly mixed, then Bacillus subtilis (0.3 percent and purchased from China Industrial microorganism culture preservation management center) and Aspergillus oryzae (0.3 percent and purchased from China Industrial microorganism culture preservation management center) are added into pure water and uniformly mixed, and then the mixture is uniformly mixed with the materials, the addition amount of the pure water is 40 percent of the dry weight of the materials, and finally the mixture is sent into a fermentation chamber.
Aerobic fermentation: and transferring the mixed materials to a fermentation chamber, controlling the temperature of the fermentation chamber at 25-30 ℃, turning the piles once every day, controlling the relative humidity of the fermentation chamber at 70-80%, and fermenting for 6 d.
After the aerobic fermentation is finished, the fermented feed is scattered, enterococcus faecalis (1 percent, purchased from China Industrial microbial strain preservation management center) is inoculated into the material, and then the material is filled into a one-way valve fermentation bag for fermentation. The temperature of the fermentation chamber is controlled to be 25-30 ℃, and the fermentation time is 32 hours.
And (3) drying: transferring the fermented material to a drying workshop, controlling the drying temperature to be 40-45 ℃, and waiting until the moisture of the material reaches 8%.
3. Preparation method of folium Eucommiae extract
Soaking folium Eucommiae in 70 deg.C water, reflux extracting, repeating for 3 times, mixing extractive solutions, concentrating under reduced pressure, and spray drying the concentrated solution with spray tower to obtain folium Eucommiae extract.
Example 2 feeding of laying hens with folium Eucommiae feed as additive
1) Mixing folium Eucommiae powder, fermented folium Eucommiae powder and folium Eucommiae extract with daily ration (laying hen compound feed 106A), and feeding with constant feeding management system.
2) Selecting high-quality layer chicken variety (Nongda seventh), feeding the layer chicken in the age of 70 days until the layer chicken is rejected.
Wherein the mixing ratio of folium Eucommiae powder, fermented folium Eucommiae powder and folium Eucommiae extract to daily ration is 2%, 4 ‰ and 2 ‰, respectively.
Example 3 feed Effect of eucommia ulmoides
First, analysis method
1. Metabolome analysis
1) Sample preparation
After the egg white and the egg yolk are respectively freeze-dried, 20mg of sample is weighed, and 1000 mul of extracting solution (methanol: acetonitrile: water: 2: 1(V/V), isotope labeling internal standard mixture is added); ultrasonic extraction, centrifuging, taking the supernatant, and detecting in a sample bottle.
2) UPLC-MS analysis method
The target compound was chromatographed on a Waters ACQUITY UPLC BEH Amide (2.1 mm. times.100 mm,1.7 μm) liquid chromatography column using Vanqish (thermo Fisher scientific) ultra performance liquid chromatography. The liquid chromatogram is characterized in that the phase A is a water phase containing 25mmol/L ammonium acetate and 25mmol/L ammonia water, and the phase B is acetonitrile. Temperature of the sample pan: 4 ℃, injection volume: 3 μ L.
Thermo Q active HFX mass spectrometer. The detailed parameters are as follows: 30Arb for shear gas flow rate, 25Arb for Aux gas flow rate, 350 ℃ for Capillary temperature, 60000 for Full MS resolution, 7500 for MS/MS resolution, 10/30/60 for fusion energy, NCE mode, 3.6kV (positive).
2. Quantitative analysis of protein, amino acid, vitamin A and elements
The protein is analyzed by a GB5009.5-2010 method, the amino acid content is analyzed by an amino acid analyzer according to a GB/T5009.124-2003 method, the vitamin A is analyzed by a GB/T5009.82-2003 method, and a plurality of elements are quantitatively analyzed by an atomic absorption spectrophotometer.
Second, analysis results
The total ion flow graph of egg white and egg yolk is shown in figures 1-4.
After the eucommia ulmoides is added into the feed, the endogenous metabolites of the eggs are obviously changed. The egg white and yolk of eucommia ulmoides egg have great difference with the metabolites in the egg white and yolk of common egg, as shown in fig. 5-8.
1. Comparison of eucommia ulmoides egg white with common egg white endogenous metabolites
Upon analysis, a total of 317 metabolites were detected from egg white as shown in table 1.
TABLE 1 comparison of metabolites in eucommia ulmoides eggs and common egg white
Figure BDA0003490364140000051
Figure BDA0003490364140000061
Figure BDA0003490364140000071
Figure BDA0003490364140000081
Figure BDA0003490364140000091
Figure BDA0003490364140000101
Figure BDA0003490364140000111
Figure BDA0003490364140000121
(1) The phospholipid content is obviously changed
As can be seen from table 1, the total of 29 phospholipids increased in the eucommia ulmoides egg white compared to the normal egg white, including PC (15 species), PE (2 species) LysoPC (10 species), LysoPE (2 species). Among them, the difference multiples of more than 2 are mainly PC (18:2(9Z,12Z)/15:0), PC (20:4(8Z,11Z,14Z,17Z)/15:0), LysoPC (16:1(9Z)), LysoPC (18:3(6Z,9Z,12Z)), LysoPC (20:1(11Z)), LysoPC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)), LysoPE (18:1(9Z)/0:0), and the like.
PC: phosphatidylcholine (phosphatidylcholine), also known as lecithin; PE: phosphatidylethanolamine (also known as cephalin); LysoPC: a lysophospholipid; LysoPE: lysophosphatidylethanolamine.
Phospholipids are lipid compounds containing phospholipid root, and play important roles in activating cells, balancing secretion of hormones, enhancing immunity and regenerative power of human bodies, and the like. In addition, phospholipid also has effects of promoting fat metabolism, preventing fatty liver, reducing serum cholesterol, improving blood circulation, and preventing cardiovascular diseases.
(2) Changes in vitamin content
As can be seen from table 1, nicotinamide (vitamin B3), riboflavin (vitamin B2), pantothenic acid (vitamin B5), and panthenol (provitamin B5) were significantly increased in the eucommia ulmoides oliver than in the common egg white.
2. Comparison of endogenous metabolites in eucommia ulmoides yolk with those in common yolk
334 metabolites were detected from egg yolk by analysis, as shown in Table 2.
TABLE 2 comparison of metabolites in the yolk of eucommia ulmoides and common eggs
Figure BDA0003490364140000122
Figure BDA0003490364140000131
Figure BDA0003490364140000141
Figure BDA0003490364140000151
Figure BDA0003490364140000161
Figure BDA0003490364140000171
Figure BDA0003490364140000181
Figure BDA0003490364140000191
Figure BDA0003490364140000201
(1) Significant change of phospholipid
As seen from Table 2, the content of 59 kinds of phospholipids, including PC (38 kinds), PE (7 kinds), LysoPC (7 kinds) and LysoPE (7 kinds), was increased in the eucommia ulmoides yolk compared to the common yolk. Among them, the difference multiples of more than 2 are mainly PC (18:1(9Z)/18:0), PC (20:4(8Z,11Z,14Z,17Z)/20:3(8Z,11Z,14Z)), PC (20:5(5Z,8Z,11Z,14Z,17Z)/P-18:1(11Z)), PC (24:1(15Z)/14:1(9Z)), and the like.
(2) The vitamin content is obviously increased
As can be seen from table 2, thiamine (vitamin B1), riboflavin (vitamin B2), niacinamide (vitamin B3), pantothenic acid (vitamin B5), biotin, and vitamin B7 were significantly increased in the yolk of eucommia ulmoides oliv as compared to the common yolk.
3. Comparison of relative contents of several important components in eucommia ulmoides egg and common egg
The comparison results of several important ingredients in the egg white and egg yolk of eucommia ulmoides egg with the common egg are shown in tables 3 and 4. The research result shows that compared with the common egg, the vitamin B complex of the eucommia ulmoides egg is obviously increased.
DHA (docosahexaenoic acid) is docosahexaenoic acid, commonly known as brain gold, is an unsaturated fatty acid which is very important to human body, is a main component for growth and maintenance of nervous system cells, and is an important constituent of brain and retina. EPA (eicosapentaenoic acid) is eicosapentaenoic acid, helps growth and development, reduces cholesterol and triglyceride, and promotes metabolism of saturated fatty acid in vivo.
As can be seen from tables 3 and 4, both DHA and EPA containing glycerophospholipids were increased, and in particular, PC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)/18:1(11Z)), LysoPC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)) in egg white and PC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)/18:1(11Z)) in egg yolk and PC (20:5(5Z,8Z,11Z,14Z,17Z)/P-18:1(11Z)) were significantly increased.
TABLE 3 comparison of the content of several important components in the eggs from eucommia ulmoides and common eggs
Figure BDA0003490364140000202
TABLE 4 comparison of the content of several important components in the yolk of eucommia ulmoides oliv and common egg
Figure BDA0003490364140000211
As can be seen from Table 5, the 7-ketocholesterol in the egg yolk of eucommia ulmoides oliv was reduced by 9.09% (7-ketocholesterol is the most common product of the reaction between cholesterol and oxygen radicals, and is the highest concentration of oxysterol in blood and arterial plaque and various other diseased tissues and cells of patients with coronary artery disease. unlike cholesterol, 7-ketocholesterol has sustained toxicity to cells and its physiological function in humans or other complex organisms is unknown).
4. Comparison of protein, amino acid, vitamin A and element contents of eucommia ulmoides egg and common egg
The contents of proteins, amino acids, vitamin a and elements in the eucommia ulmoides chicken eggs and the common chicken eggs are shown in table 5.
TABLE 5 comparison of the protein, amino acid and vitamin A and element content of eucommia ulmoides oliv and common egg
Figure BDA0003490364140000212
Figure BDA0003490364140000221
As can be seen from Table 5, compared with the common eggs, the eucommia ulmoides eggs have the advantages that the protein is increased by 13.47%, the amino acid is increased by 36.4%, the vitamin A is increased by 12.15%, the selenium is increased by 58.47%, the calcium is increased by 62.92%, the zinc is increased by 7.14%, and the iron is increased by 10.80%. Among the detected amino acids, the content of 7 essential amino acids in the eucommia ulmoides egg is increased. In addition, the histidine content in the eucommia ulmoides eggs is increased by 61.11 percent compared with the common eggs. Because children cannot synthesize histidine and need to take the histidine from food, the eucommia ulmoides olives are particularly suitable for children to eat.
Lead, mercury and cadmium are not detected in the eucommia ulmoides eggs and the common eggs, and the product is safe and reliable.
5. Comparison of color and taste of eucommia ulmoides egg and common egg
Compared with the common eggs, the eucommia ulmoides egg is clear and transparent in egg white and fresh and yellow in yolk, as shown in fig. 9.
After 20 people eat the eggs, the eucommia ulmoides oliv eggs are considered to have more delicate and smooth mouthfeel than the common eggs.
As can be seen from the above experimental results, the leaves of eucommia ulmoides have a significant effect on the endogenous metabolites of eggs. Compared with common eggs, the vitamin B group of the eucommia ulmoides eggs is obviously increased, the glycerophospholipids containing DHA and EPA are all increased, and particularly, the increase of PC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)/18:1(11Z)), LysoPC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)) in egg white and PC (22:6(4Z,7Z,10Z,13Z,16Z,19Z)/18:1(11Z)) in egg yolk and PC (20:5(5Z,8Z,11Z,14Z,17Z)/P-18:1(11Z)) in egg yolk is very obvious, and the 7-ketocholesterol in the eucommia ulmoides eggs is reduced. The total amount of protein and amino acid, vitamin A, selenium, calcium, zinc and iron in the eucommia ulmoides eggs are all obviously increased, the content of 7 essential amino acids is all increased, and the content of histidine is obviously increased. Therefore, the eucommia ulmoides eggs are rich in nutrition, safe and high in quality, are suitable for being eaten by multiple people such as hyperlipidemia and are particularly suitable for being eaten by children.

Claims (10)

1. A preparation method of fermented eucommia ulmoides leaf powder comprises the following steps:
s1, crushing the eucommia leaves to obtain eucommia leaf powder, and then steaming;
s2, adding Aspergillus niger into the eucommia ulmoides leaf powder obtained in the step S1, and uniformly mixing; then adding the aqueous solution of bacillus subtilis and aspergillus oryzae to obtain a mixed material; carrying out aerobic fermentation on the mixed material;
s3, inoculating enterococcus faecalis into the aerobically fermented material, continuing to ferment, and drying the fermented material to obtain the enterococcus faecalis.
2. The method of claim 1, wherein: in step S1, the folium cortex eucommiae powder is sieved by a 40-mesh sieve;
the steaming conditions are as follows: the temperature is 60-80 ℃, and the time is 20-30 min;
in step S2, the inoculation amount of Aspergillus niger is 3% -8%;
the inoculation amount of the bacillus subtilis is 0.1-0.5%, and the inoculation amount of the aspergillus oryzae is 0.1-0.5%;
the addition amount of water is 40-50% of the dry weight of the eucommia ulmoides leaf powder.
3. The method of manufacturing according to claim 1 or, characterized in that: in step S2, the aerobic fermentation conditions are:
turning the pile once every day at the temperature of 25-30 ℃, controlling the relative humidity of the fermentation chamber to be 70-80%, and fermenting for 5-7 d;
in the step S3, the inoculation amount of the enterococcus faecalis is 1-5%;
the fermentation conditions are as follows: the temperature is 25-30 ℃, and the time is 24-36 h;
the drying conditions are as follows: drying at 40-45 ℃ until the water content of the material reaches 5% -10%.
4. Fermented eucommia ulmoides leaf powder prepared by the method of any one of claims 1 to 3.
5. Use of folium Eucommiae powder, the fermented folium Eucommiae powder and/or folium Eucommiae extract of claim 4 as additive for layer chicken feed;
the eucommia ulmoides leaf extract is prepared by the following method: soaking folium Eucommiae in water, reflux extracting, concentrating the obtained extractive solution, and drying;
the eucommia ulmoides leaf powder is sieved by a 40-mesh sieve.
6. Use according to claim 5, characterized in that: the additive amount of the laying hen feed is as follows:
the amount of the eucommia leaf powder is 1 to 3.5 percent of the daily ration of the laying hen;
the amount of the fermented eucommia ulmoides leaf powder is 2-5 per mill of the daily ration of the laying hens;
the amount of the eucommia ulmoides leaf extract is 1-2 per mill of the daily ration of the laying hens.
7. A layer feed additive, which is eucommia ulmoides leaf powder, the fermented eucommia ulmoides leaf powder and/or the eucommia ulmoides leaf extract of claim 4;
the mass ratio of the eucommia ulmoides leaf powder to the fermented eucommia ulmoides leaf powder to the eucommia ulmoides leaf extract is 10-35: 2-5: 1.
8. a layer feed, comprising layer ration and an additive, wherein the additive is eucommia ulmoides leaf powder, the fermented eucommia ulmoides leaf powder and/or the eucommia ulmoides leaf extract according to claim 4.
9. The laying hen feed of claim 8, wherein: the amounts of the additives were as follows:
the amount of the eucommia ulmoides leaf powder is 1 to 3.5 percent of the daily ration of the laying hen;
the amount of the fermented eucommia ulmoides leaf powder is 2-5 per mill of the daily ration of the laying hens;
the amount of the eucommia ulmoides leaf extract is 1-2 per mill of the daily ration of the laying hens.
10. A production method of eucommia ulmoides egg comprises the following steps:
feeding laying hens by using a laying hen daily ration until the laying hens are 70-75 days old, and then feeding the laying hens by using the laying hen feed according to claim 8 or 9 to obtain the eucommia ulmoides eggs.
CN202210094685.5A 2022-01-26 2022-01-26 Eucommia ulmoides egg and production method thereof Pending CN114451487A (en)

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