CN108094726A - A kind of creep feed for enhancing pigling immunity and preparation method and application - Google Patents
A kind of creep feed for enhancing pigling immunity and preparation method and application Download PDFInfo
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- CN108094726A CN108094726A CN201711343873.2A CN201711343873A CN108094726A CN 108094726 A CN108094726 A CN 108094726A CN 201711343873 A CN201711343873 A CN 201711343873A CN 108094726 A CN108094726 A CN 108094726A
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- creep feed
- fermentation
- purslane
- piglet
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- 238000010241 blood sampling Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000004534 cecum Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- ZAKOWWREFLAJOT-UHFFFAOYSA-N d-alpha-Tocopheryl acetate Natural products CC(=O)OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-UHFFFAOYSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 125000004494 ethyl ester group Chemical group 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- FOYKKGHVWRFIBD-UHFFFAOYSA-N gamma-tocopherol acetate Natural products CC(=O)OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1 FOYKKGHVWRFIBD-UHFFFAOYSA-N 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000003228 hemolysin Substances 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 210000003405 ileum Anatomy 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 150000002669 lysines Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 150000003588 threonines Chemical class 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
- A23K10/28—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin from waste dairy products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A23V2400/11—Lactobacillus
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Abstract
The present invention relates to a kind of creep feeds for enhancing pigling immunity and preparation method and application, belong to field of fodder.Its preparation method includes fermentation purslane grass, is then mixed with basic creep feed.Basic creep feed includes corn, whey powder, dregs of beans, fish meal, sucrose, full-fat bean, soya-bean oil, mountain flour, calcium monohydrogen phosphate, amino acid, organic micro substance, salt, multidimensional substance, choline, acidulant and flavouring agent.The preparation method is simple, easy to operate, can largely release the Polysaccharide from Portulaca oleracea in purslane.The creep feed that is prepared by above-mentioned preparation method is cheap, at low cost, high efficiency, uses it for feeding piglet and is remarkably improved piglet immunological shoot formation, enhances pigling immunity, reduce diarrhea of weaned piglets, it is horizontal to improve piglet vaccine immunity.
Description
Technical field
The present invention relates to field of fodder, and more particularly to a kind of creep feed and preparation method thereof for enhancing pigling immunity with
Using.
Background technology
Currently, pig raising technology is quickly grown, and in order to pursue the maximization of sow benefit, weaned piglet age in days is more and more early,
However premature wean, cause piglet immunological allelotaxis incomplete, the deficiency of itself resistance, in addition outside intensive raising
Portion's disease pressure is big, and weanling pig Post Weaning Diarrhea is caused to happen occasionally, and desirable level, disease is also not achieved in immune effect of vaccine
Explosion pressure is big, and great threat is brought to pig production.
A large amount of uses of previous antibiotic, cause a large amount of generations of drug-fast bacteria, seriously endanger health.As nonreactive is raised
The propulsion of material further increases the difficulty of disease control in breeding production.The characteristics of Chinese medicine is safe efficient makes it add in feed
Add the application in agent increasingly common.But most Chinese herbal feed additive formula is complicated, and part price of medicinal material is expensive, practical
Property is not strong.
Therefore, new exploration need to be carried out to obtain the preferable pig starter feed of cost performance.
The content of the invention
The first object of the present invention is to provide a kind of preparation method for the creep feed for enhancing pigling immunity, the preparation side
Method is simple, easy to operate, can largely release the Polysaccharide from Portulaca oleracea in purslane.
The second object of the present invention is to provide a kind of creep feed being prepared by above-mentioned preparation method, the creep feed valency
Lattice are cheap, at low cost, high efficiency.
The third object of the present invention is to provide a kind of application of above-mentioned creep feed, uses it for feeding piglet, can improve
Piglet immunological shoot formation enhances pigling immunity, reduces diarrhea of weaned piglets, and it is horizontal to improve piglet vaccine immunity.
The present invention is solved its technical problem and is realized using following technical scheme：
The present invention proposes a kind of preparation method for the creep feed for enhancing pigling immunity, comprises the following steps：Fermentation dent
Amaranth grass, then mixes with basic creep feed.
Basic creep feed include corn, whey powder, dregs of beans, fish meal, sucrose, full-fat bean, soya-bean oil, mountain flour, calcium monohydrogen phosphate,
Amino acid, organic micro substance, salt, multidimensional substance, choline, acidulant and flavouring agent.
Preferably, the purslane grass after fermentation and basic creep feed are (2 by weight：98)-(6：94) mix.
The present invention also proposes the creep feed that a kind of preparation method by above-mentioned creep feed is prepared.
The present invention also proposes the application of above-mentioned creep feed, such as can use it for feeding piglet.
The creep feed and preparation method and application for the enhancing pigling immunity that present pre-ferred embodiments provide it is beneficial
Effect is：
Present pre-ferred embodiments offer preparation method is simple, easy to operate, can largely release in purslane
Polysaccharide from Portulaca oleracea.
It is careless by adding in fermentation purslane in basic creep feed, feed cost on the one hand can be reduced, on the other hand, horse
Bitterroot is medicinal and edible plant, and the common bitter taste of no Chinese herbal medicine, palatability is good, will not reduce the feed intake of piglet.Obtained religion
Slot material is cheap, at low cost, high efficiency.
Feeding piglet is used it for, piglet immunological shoot formation can be improved, enhances pigling immunity, reduces weanling pig abdomen
It rushes down, it is horizontal to improve piglet vaccine immunity.
Specific embodiment
It, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Creep feed of enhancing pigling immunity of the embodiment of the present invention and preparation method and application is carried out below specific
Explanation.
The preparation method of the creep feed of enhancing pigling immunity in the embodiment of the present invention comprises the following steps：Fermentation dent
Amaranth grass, then mixes with basic creep feed.Preferably, the purslane grass after fermentation and basic creep feed are (2 by weight：
98)-(6：94) mix.Under this proportioning, purslane grass and basic creep feed collective effect best results carry pigling immunity
Ascending effect is apparent.
Purslane is a kind of Common Wild Vegetables grown in field, has thousands of years of applicating history in China, is health
One of wild plant of integration of drinking and medicinal herbs in 78 as defined in portion.Purslane contains abundant nutrition, and spy is inorganic elements calcium, magnesium, potassium,
Organic micro substance iron, manganese, zinc, Se content are higher.Also, purslane is also containing abundant active ingredient, such as polysaccharide, tonka-bean
Plain class, alkaloids, flavonoids, volatile oil etc..Purslane has different physiological roles, such as removes free radical, oxidation resistance,
Improve immunity of organisms, inhibitory anti-virus and other effects.
Wherein, Polysaccharide from Portulaca oleracea can be obviously promoted the phagocytic function of external peritoneal macrophage and NO and cell factor
The generation of IL-1.Since Macrophage Surface is there are polysaccharide receptor, it is thin that Polysaccharide from Portulaca oleracea can then be combined activation macrophage with receptor
Born of the same parents, promote macrophages secrete NO and release effects factor IL-1, with improve the phagocytic percentage of piglet peritoneal macrophage and
Phagocytic index promotes the formation of hemolysin and hemolysis plaque, promotes the conversion of lymphocyte, so as to improve the immunity energy of piglet
Power.
The embodiment of the present invention by basic creep feed add in fermentation purslane grass, on the one hand can reduce feed into
This, on the other hand, purslane is medicinal and edible plant, and the common bitter taste of no Chinese herbal medicine, palatability is good, will not reduce adopting for piglet
Appetite.
Specifically, fermentation is that zymophyte is inoculated in purslane grass, then anaerobic fermentation.It uses and detests in the embodiment of the present invention
Aerobe fermentation can release more Polysaccharide from Portulaca oleracea compared with aerobic fermentation.Optionally, above-mentioned zymophyte include saccharomycete with
Bacillus acidi lactici, the weight ratio of two kinds of strains can be 3：4-10 namely Bacillus acidi lactici are main zymophyte.By by saccharomycete with
Ferment purslane grass after Bacillus acidi lactici mixing, can be obtained more only with better obtained by any of which strain fermentation
More Polysaccharide from Portulaca oleracea.
Saccharomycete and Bacillus acidi lactici are during the fermentation there are metabolite complementation phenomenon, since lactic acid bacteria is auxotrophy
Type bacterial strain, the macromolecular substances that saccharomycete generates can induce the aminopeptidase in lactic acid bacteria to synthesize, make the amino in culture solution
Acid and small molecule peptide content increase, so as to promote lactobacter growth.Also, saccharomycete is more complete containing species in itself
Amino acid is good protein source, is utilized for lactobacter growth.
Accordingly, lactic acid bacteria also has facilitation for Yeast Growth, such as lactic acid bacteria can produce during the fermentation
Raw monose, free amino acid and the multivitamin for being easy to absorb, these small-molecule substances can provide rich for the growth of saccharomycete
Rich source of nutrition.
To ensure attenuation degree and ferment effect, it is preferable that the viable count of above-mentioned saccharomycete and Bacillus acidi lactici is not less than
10000000000/g.Zymogenic inoculum concentration can be controlled in the range of 0.6-1wt% namely zymogenic inoculum concentration is purslane grass
Weight 0.6-1%.This inoculation scope can make that zymophyte and purslane raw material proportioning are proper, and the resultant effect of fermentation is optimal.
It is preferred that fermentation carries out 5-7 days under conditions of being 4.8-5.2 in 28-32 DEG C, pH value in the embodiment of the present invention.Hair
During ferment, sugar source (such as sucrose) and water are added in.Optionally, sugar source and the addition of water are respectively the 2-3wt% of purslane grass
And 60-65wt%.It can make purslane grass under this fermentation condition while its original nutritional ingredient is retained, fermentation obtains most
Polysaccharide from Portulaca oleracea.
To make the ferment effect of purslane grass more preferably, before fermentation, purslane grass meal is preferably broken to grain size less than 300 μm,
To increase contact and response area of the purslane grass with fermenting microbe, shorten fermentation time, improve the yield of fermentate.
Optionally, the basic creep feed in the embodiment of the present invention may include corn, whey powder, dregs of beans, fish meal, sugarcane
Sugar, full-fat bean, soya-bean oil, mountain flour, calcium monohydrogen phosphate, amino acid, organic micro substance, salt, multidimensional substance, choline, acidulant and
Flavouring agent.
The weight ratio of above-mentioned raw materials ingredient can be followed successively by 50-62：10-20：10-18：2-4：2-4：2-4：2-2.4：
0.4-0.8：0.6-1：1.05-1.45：0.3-0.34：0.1-0.3：0.01-0.03：0.05-0.15：0.3-0.7：0.005-
0.015。
Wherein, corn and dregs of beans are mainly that piglet provides carbohydrate, and whey powder and fish meal are mainly that piglet provides egg
White matter, full-fat bean and soya-bean oil are mainly that piglet provides energy.
Corn may include untreated corn and popcorn, and popcorn is changed through expanding treatment later stage sugar structure
Become, be more conducive to piglet and digest and assimilate.Dregs of beans may include fermented bean dregs and dehulled soybean meal, and wherein fermented bean dregs can by fermentation
Gas chromatography contained in dregs of beans is decomposed, to be more easy to be absorbed by piglet.
Amino acid includes lysine, methionine, threonine and tryptophan.The weight ratio of above-mentioned four kinds of amino acid can be successively
For 0.45-0.55：0.1-0.2：0.05-0.15：0.45-0.55 is preferably 0.5：0.15：0.1：0.5.
Organic micro substance is by Conditions of copper-Compound Amino acids, ferrous glycine, selenium methionine, chromium methionine, glycine zine, compound ammonia
Base acid manganese and calcium iodate composition, to provide copper, iron, selenium, chromium, zinc, manganese and iodine.It is preferred that contain per the organic micro substances of kg
There are the zinc of 26800-26900mg, the iron of 26250-26300mg, the manganese of 12800-13000mg, the copper of 6700-6760mg, 370-
The chromium of the iodine of 400mg, the selenium of 80-100mg and 40-60mg.More preferably, per the organic micro substances of kg contain 26855mg zinc,
26280mg iron, 12900mg manganese, 6730mg copper, 385mg iodine, 92mg selenium and 52mg chromium.
Multidimensional substance includes retinyl acetate, vitamine D3, tocopherol acetate, niacinamide, D-VB5 calcium, dimension life
Plain B1, vitamin B2, vitamin B6, vitamin B12, vitamin K, folic acid and D-Biotin.It is preferred that contain per kg multidimensional substances
There are the retinyl acetate of ten thousand IU of 3000-3400, the vitamine D3 of ten thousand IU of 1000-1100, the tocopheryl acetate of 200-220g
Ester, the niacinamide of 110-130g, the D-VB5 calcium of 90-110g, the vitamin B1 of 8-16g, vitamin B2, the 10-22g of 20-28g
Vitamin B6, the vitamin B12 of 0.1-0.2g, the vitamin K of 5-15g, the folic acid of 4-6g and the D-Biotin of 0.6-1g.
More preferably, per kg multidimensional substances contain the retinyl acetate of 32,000,000 IU, 10,500,000 IU vitamine D3,
The tocopherol acetate of 210g, the niacinamide of 118g, the D-VB5 calcium of 98g, the vitamin B1 of 12g, vitamin B2, the 16g of 24g
Vitamin B6, the vitamin B12 of 0.15g, the vitamin K of 10g, the folic acid of 4.8g and the D-Biotin of 0.8g.
It is preferred that 36kg corns, 20kg popcorns, 15kg whey powders, 8kg can be contained in per 100kg bases creep feed
Fermented bean dregs, 6kg dehulled soybean meals, 3kg fish meal, 3kg sucrose, 3kg full-fat beans, 2.2kg soya-bean oil, 0.6kg mountain flours (120 mesh),
0.8kg calcium monohydrogen phosphates, 0.5kg lysines (98%), 0.32kg piglets are organic micro, 0.2kg salt, 0.02kg piglet multidimensional,
0.15kg methionine, 0.1kg threonines, 0.5kg tryptophans, 0.1kg choline, 0.5kg acidulants, 0.01kg flavouring agents.
Since the digestion power of piglet is effective, by adding in acidulant in basic creep feed in the embodiment of the present invention, the
One can improve the dissolubility and digestibility of the nutritional ingredient of creep feed, and second can reduce ileum and caecum toxicity polyamines and ammonia
Generation, the 3rd can inhibit growth of the optimum growh pH value in the viral bacterium of neutral range (6.5-7.5).
Optionally, above-mentioned acidulant can for example include formic acid, fumaric acid and lactic acid.Preferably, when simultaneously including first
When acid, fumaric acid and lactic acid, weight ratio is respectively 1：1-3：1-3；More preferably, the weight ratio of three's substance is followed successively by 1：
2：2.Wherein, lactic acid has high nutritive value, smell is weak, palatability is good, antibacterial effect is apparent.
Flavouring agent includes natural perfume material extract, ethylmaltol, Ethyl vanillin, mandarin oil, isoamyl acetate, acetic acid
At least one of ethyl ester and synergist.By adding in flavouring agent, the palatability of creep feed can be improved, son is improved so as to reach
The effect of the feed intake of pig.
The embodiment of the present invention also provides the application of creep feed namely uses it for feeding piglet.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
Zymophyte is inoculated in purslane grass for 0.6wt% by inoculum concentration, in 28 DEG C, the condition fermentation 7 that pH value is 4.8
My god, must ferment purslane grass.Fermentation process adds in the sucrose and water of 2wt% and 60wt%.Wherein, zymophyte includes weight ratio
For 3：4 saccharomycete and Bacillus acidi lactici, the viable count of two kinds of fermenting microbes is 10,000,000,000/g.
It is 2 by weight：Purslane grass and basic creep feed after 98 mixed fermentations must enhance the religion slot of pigling immunity
Material.
Wherein, basic creep feed contains weight ratio for 50：10：10：2：2：2：2：0.4：0.6：1.05：0.3：0.1：
0.01：0.05：0.3：0.005 corn, whey powder, dregs of beans, fish meal, sucrose, full-fat bean, soya-bean oil, mountain flour, calcium monohydrogen phosphate,
Amino acid, organic micro substance, salt, multidimensional substance, choline, acidulant and flavouring agent.
It is 30 that corn, which includes weight ratio,：20 conventional corn and popcorn, it is 6 that dregs of beans, which includes weight ratio,：6 fermentation beans
The dregs of rice and dehulled soybean meal.It is 0.45 that amino acid, which includes weight ratio,：0.1：0.05：0.45 lysine, methionine, threonine and color ammonia
Acid.It is 1 that acidulant, which includes weight ratio,：1：1 formic acid, fumaric acid and lactic acid.Flavouring agent includes natural perfume material extract.
Per the organic micro substances of kg contain the zinc of 26800mg, the iron of 26250mg, the manganese of 12800mg, 6700mg copper,
The chromium of the iodine of 370mg, the selenium of 80mg and 40mg.Above-mentioned each element respectively by glycine zine, ferrous glycine, compound amino acid manganese,
Conditions of copper-Compound Amino acids, calcium iodate, selenium methionine and chromium methionine provide.
Contain the fertility of the retinyl acetate of 30,000,000 IU, the vitamine D3 of 10,000,000 IU, 200g per kg multidimensional substances
Phenol acetic acid esters, the niacinamide of 110g, the D-VB5 calcium of 90g, the vitamin B1 of 8g, the vitamin B2 of 20g, 10g vitamin B6,
The vitamin B12 of 0.1g, the vitamin K of 5g, the D-Biotin of the folic acid of 4g and 0.6g.
Embodiment 2
Zymophyte is inoculated in purslane grass for 1wt% by inoculum concentration, is fermented 5 days in 32 DEG C, the condition that pH value is 5.2,
Must ferment purslane grass.Fermentation process adds in the sucrose and water of 3wt% and 65wt%.Wherein, it is 3 that zymophyte, which includes weight ratio,：
10 saccharomycete and Bacillus acidi lactici, the viable count of two kinds of fermenting microbes is respectively 10,000,000,000/g and 12,000,000,000/g.
It is 6 by weight：Purslane grass and basic creep feed after 94 mixed fermentations must enhance the religion slot of pigling immunity
Material.
Basic creep feed contains weight ratio for 62：20：18：4：4：4：2.4：0.8：1：1.45：0.34：0.3：0.03：
0.15：0.7：0.015 corn, whey powder, dregs of beans, fish meal, sucrose, full-fat bean, soya-bean oil, mountain flour, calcium monohydrogen phosphate, amino
Sour, organic micro substance, salt, multidimensional substance, choline, acidulant and flavouring agent.
It is 42 that corn, which includes weight ratio,：20 conventional corn and popcorn, it is 12 that dregs of beans, which includes weight ratio,：6 fermentation
Dregs of beans and dehulled soybean meal.It is 0.55 that amino acid, which includes weight ratio,：0.2：0.15：0.55 lysine, methionine, threonine and color
Propylhomoserin.It is 1 that acidulant, which includes weight ratio,：3：3 formic acid, fumaric acid and lactic acid.It is fragrant that flavouring agent includes ethylmaltol, ethyl
Lan Su and mandarin oil.
Per the organic micro substances of kg contain the zinc of 26900mg, the iron of 26300mg, the manganese of 13000mg, 6760mg copper,
The chromium of the iodine of 400mg, the selenium of 100mg and 60mg.Above-mentioned each element respectively by glycine zine, ferrous glycine, compound amino acid manganese,
Conditions of copper-Compound Amino acids, calcium iodate, selenium methionine and chromium methionine provide.
Contain the fertility of the retinyl acetate of 34,000,000 IU, the vitamine D3 of 11,000,000 IU, 220g per kg multidimensional substances
Phenol acetic acid esters, the niacinamide of 130g, the D-VB5 calcium of 110g, the vitamin B1 of 16g, the vitamin B2 of 28g, the vitamin of 22g
The vitamin B12 of B6,0.2g, the vitamin K of 15g, the D-Biotin of the folic acid of 6g and 1g.
Embodiment 3
Zymophyte is inoculated in purslane grass for 0.8wt% by inoculum concentration, in 30 DEG C, the condition fermentation 6 that pH value is 5.0
My god, must ferment purslane grass.Fermentation process adds in the sucrose and water of 2.5wt% and 62.5wt%.Wherein, zymophyte includes weight
Amount is than being 3：7 saccharomycete and Bacillus acidi lactici, the viable count of two kinds of fermenting microbes is respectively 15,000,000,000/g and 13,000,000,000/g.
It is 4 by weight：Purslane grass and basic creep feed after 96 mixed fermentations must enhance the religion slot of pigling immunity
Material.
Wherein, basic creep feed contains weight ratio for 56：15：14：3：3：3：2.2：0.6：0.8：1.25：0.32：0.2：
0.02：0.1：0.5：0.01 corn, whey powder, dregs of beans, fish meal, sucrose, full-fat bean, soya-bean oil, mountain flour, calcium monohydrogen phosphate, ammonia
Base is sour, organic micro substance, salt, multidimensional substance, choline, acidulant and flavouring agent.
It is 36 that corn, which includes weight ratio,：20 conventional corn and popcorn, it is 8 that dregs of beans, which includes weight ratio,：6 fermentation beans
The dregs of rice and dehulled soybean meal.It is 0.5 that amino acid, which includes weight ratio,：0.15：0.1：0.5 lysine, methionine, threonine and color ammonia
Acid.It is 1 that acidulant, which includes weight ratio,：2：2 formic acid, fumaric acid and lactic acid.Flavouring agent includes natural perfume material extract, acetic acid
Isopentyl ester, ethyl acetate and synergist.
Per the organic micro substances of kg contain 26855mg zinc, 26280mg iron, 12900mg manganese, 6730mg copper, 385mg iodine,
92mg selenium and 52mg chromium.Above-mentioned each element is respectively by glycine zine, ferrous glycine, compound amino acid manganese, Conditions of copper-Compound Amino acids, iodine
Sour calcium, selenium methionine and chromium methionine provide.
Contain the fertility of the retinyl acetate of 32,000,000 IU, the vitamine D3 of 10,500,000 IU, 210g per kg multidimensional substances
Phenol acetic acid esters, the niacinamide of 118g, the D-VB5 calcium of 98g, the vitamin B1 of 12g, the vitamin B2 of 24g, the vitamin of 16g
The vitamin B12 of B6,0.15g, the vitamin K of 10g, the D-Biotin of the folic acid of 4.8g and 0.8g.
Embodiment 4
Difference lies in the present embodiment before fermentation, purslane grass meal is broken to grain size is the present embodiment with embodiment 3
200μm。
Test example 1
Repeat to implement above-described embodiment 1-4, obtain enough creep feeds, by taking the creep feed of the gained of embodiment 4 as an example into
Row tests below.Culture experiment is carried out in Fujian Ao Nong biotechnologies Group Plc subordinate cultivation base, this experiment
Using single factor experiment, 40 nest piglets of the same unit in certain pig farm is selected to be randomly divided into tetra- groups of A, B, C, D as subjects,
Every group of 10 nests repeat 3 units.B, C, D group piglet feed the creep feed of the purslane containing fermentation, wherein C groups feeding embodiment 4
The creep feed (content of fermentation purslane grass is 4wt%) of gained, B groups, D groups and C groups difference lies in：It ferments in B groups dent
The content of amaranth grass is 2wt%, and the content for the purslane grass that ferments in C groups is 6wt%.The piglet of A groups only feeds basic creep feed (no
Fermentation purslane in group containing C).
Supplementary feeding mode is：Since the 7th day sow postpartum, when sow puts milk, a small amount of powder is spread on sow breast
Piggy is allowed to adapt to feed taste, it is 3-4 times daily；Beginning in 14 days is fed in piggy hopper on a small quantity, and the feed in hopper is daily more
It changes, until 21 days wean；Piggy is divided into four column homes by grouping after wean, feeds corresponding creep feed, free choice feeding, after 10 days
Replace child care material.
Piggy is immunized according to normal immunological program, 25 ages in days just exempt from swine fever.Experiment proceeds to 55 after piggy birth
My god, record each group piggy counterpoise of weighing；Blood sampling detection hog cholera antibody is horizontal；Every group is randomly selected 10 piggys, and solution takes thymus gland
It weighs with spleen.Record death condition, diarrhea situation of each group piggy etc..Its result of the test is as shown in table 1-3.
Table 1 tests piglet sample number, death toll and diarrhea number statistical form
| Group | Sample number (head) | Death toll (head) | Diarrhea number (head) |
| A groups | 317 | 43 | 22 |
| B groups | 320 | 29 | 15 |
| C groups | 318 | 24 | 12 |
| D groups | 321 | 25 | 13 |
Table 2 tests piglet survival ratio, diarrhea rate and counterpoise statistical form
| Group | Survival rate (%) | Diarrhea rate (%) | Counterpoise (kg) |
| A groups | 86.44 | 6.94 | 22.11 |
| B groups | 90.94 | 4.69 | 23.17 |
| C groups | 92.45 | 3.77 | 23.32 |
| D groups | 92.21 | 4.05 | 23.29 |
Table 3 tests piglet thymus gland counterpoise, spleen counterpoise and hog cholera antibody blocking rate statistical form
It is equal that compared with A groups the survival rate of piglet, counterpoise, thymus gland can be significantly improved by B-D groups in tetra- groups of A-D it can be seen from table 1-3
Weight, spleen counterpoise and hog cholera antibody blocking rate, and the diarrhea rate of piglet can be substantially reduced simultaneously.Illustrate to add in basic creep feed
The purslane that enters to ferment can effectively improve piglet immunological shoot formation, enhance pigling immunity, reduce diarrhea of weaned piglets, improve
Piglet vaccine immunity is horizontal.B-D groups, the best results of C groups are compared, D groups are taken second place, and therefore deduce that fermentation purslane grass addition
It measures and is more suitable for improving piglet growth performance for 4wt%.
Test example 2
Control group 1-4 is set, and difference lies in purslane grass additions of fermenting in control group 1-4 with embodiment 4 by control group 1-4
Amount is respectively 1wt%, 3wt%, 5wt% and 6wt%.Above-mentioned 4 groups of control groups are tested according to the method for test example 1.
Itself the results show that with fermentation purslane grass additive amount increase, the comprehensive function that creep feed plays piglet is in elder generation
Downward trend namely effect are 3 ＞ control groups of embodiment, 2 ＞ control groups 1 after rising, 3 ＞ control groups of embodiment, 3 ＞ control groups
4.It can thus be seen that the purslane grass additive amount that ferments in the present invention program is preferably in 2wt%.
Test example 3
Control group 5-12 is set, control group 5-12 and embodiment 4 difference lies in：Zymophyte is free of yeast in control group 5
Bacterium, zymophyte is without Bacillus acidi lactici in control group 6, and zymophyte contains weight ratio for 3 in control group 7:1 saccharomycete and lactic acid bar
Bacterium, zymophyte contains weight ratio for 3 in control group 8:3 saccharomycete and Bacillus acidi lactici, zymophyte contains weight ratio in control group 9
For 3:4 saccharomycete and Bacillus acidi lactici, zymophyte contains weight ratio for 3 in control group 10:10 saccharomycete and Bacillus acidi lactici, it is right
Contain weight ratio according to zymophyte in group 11 for 3:12 saccharomycete and Bacillus acidi lactici, zymophyte contains weight ratio and is in control group 12
3:15 saccharomycete and Bacillus acidi lactici.Polysaccharide from Portulaca oleracea in the fermentation purslane of above-mentioned 8 groups of control groups and embodiment 4 is contained
Amount is measured, and result is as follows successively：2.59%th, 2.12%, 6.40%, 6.95%, 7.28%, 7.43%, 7.16%,
6.83%th, 6.07%.
It can thus be seen that zymophyte is simultaneously containing saccharomycete and Bacillus acidi lactici more only containing the dent obtained by one of which
The amount of amaranth polysaccharide is more, and the weight ratio of above two strain is 3：4-10 is more compared with below or above the purslane obtained by the scope
The amount of sugar is more.
In conclusion the preparation method of creep feed provided by the invention is simple, and it is easy to operate, it can largely discharge and go into action
Polysaccharide from Portulaca oleracea in bitterroot.The creep feed that is prepared by above-mentioned preparation method is cheap, at low cost, high efficiency, by it
Piglet immunological shoot formation is remarkably improved for feeding piglet, enhances pigling immunity, reduces diarrhea of weaned piglets, is improved young
Pig vaccine immune level.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of preparation method for the creep feed for enhancing pigling immunity, which is characterized in that comprise the following steps：Fermentation purslane
Then grass is mixed with basic creep feed；
It is described basis creep feed include corn, whey powder, dregs of beans, fish meal, sucrose, full-fat bean, soya-bean oil, mountain flour, calcium monohydrogen phosphate,
Amino acid, organic micro substance, salt, multidimensional substance, choline, acidulant and flavouring agent；
Preferably, the purslane grass after fermentation and the basic creep feed are (2 by weight：98)-(6：94) mix.
2. preparation method according to claim 1, which is characterized in that fermentation is that zymophyte is inoculated in the purslane
Grass, then anaerobic fermentation；
It is 3 that the zymophyte, which includes weight ratio,：The saccharomycete and Bacillus acidi lactici of 4-10；
Preferably, the viable count of the saccharomycete and the Bacillus acidi lactici is not less than 10,000,000,000/g.
3. preparation method according to claim 2, which is characterized in that the zymogenic inoculum concentration is purslane grass
Weight 0.6-1%.
4. preparation method according to claim 1, which is characterized in that fermentation is in 28-32 DEG C, pH value 4.8-5.2
Under the conditions of carry out 5-7 days.
5. preparation method according to claim 1, which is characterized in that before fermentation, the purslane grass meal is broken to grain size
Less than 300 μm.
6. preparation method according to claim 1, which is characterized in that organic micro substance described in per kg contains 26800-
The zinc of 26900mg, the iron of 26250-26300mg, the manganese of 12800-13000mg, the copper of 6700-6760mg, 370-400mg
The chromium of iodine, the selenium of 80-100mg and 40-60mg.
7. preparation method according to claim 1, which is characterized in that the acidulant includes formic acid, fumaric acid and breast
Acid；
Preferably, the weight ratio of the formic acid, the fumaric acid and the lactic acid is 1：1-3：1-3；
More preferably, the weight ratio of the formic acid, the fumaric acid and the lactic acid is 1：2：2.
8. preparation method according to claim 1, which is characterized in that the flavouring agent includes natural perfume material extract, second
At least one of base maltol, Ethyl vanillin, mandarin oil, isoamyl acetate, ethyl acetate and synergist.
9. a kind of creep feed for enhancing pigling immunity, which is characterized in that the creep feed is by such as any one of claim 1-8 institutes
The preparation method stated is prepared.
10. the application of creep feed as claimed in claim 9, which is characterized in that be used to the creep feed feed piglet.
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