CN114432387A

CN114432387A - Traditional Chinese medicine composition with antibacterial, anti-inflammatory and antiviral effects and preparation method and application thereof

Info

Publication number: CN114432387A
Application number: CN202210176912.9A
Authority: CN
Inventors: 周威; 赵岩岩; 张�浩; 崔震昆; 刘本国
Original assignee: Henan Institute of Science and Technology
Current assignee: Henan Institute of Science and Technology
Priority date: 2022-02-24
Filing date: 2022-02-24
Publication date: 2022-05-06

Abstract

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses an antibacterial, anti-inflammatory and antiviral traditional Chinese medicine composition, and a preparation method and application thereof, wherein the traditional Chinese medicine composition is prepared from the following components in parts by weight: 10-20 parts of honeysuckle, 2-8 parts of mint, 3-9 parts of safflower, 6-12 parts of hawthorn, 15-25 parts of bunge corydalis herb, 6-12 parts of tree peony bark, 3-9 parts of common monkshood mother root and 6-10 parts of eucommia bark. The traditional Chinese medicine composition prepared by the invention has stronger inhibition on staphylococcus aureus, escherichia coli and beta hemolytic streptococcus, and has the best inhibition effect on staphylococcus aureus.

Description

Traditional Chinese medicine composition with antibacterial, anti-inflammatory and antiviral effects and preparation method and application thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicines, in particular to an antibacterial, anti-inflammatory and antiviral traditional Chinese medicine composition and a preparation method and application thereof.

Background

Inflammation is a very common and important basic pathological process, and trauma infection on the body surface and most common diseases and frequently encountered diseases of various organs (such as furuncle, carbuncle, pneumonia, hepatitis, nephritis and the like) belong to inflammatory diseases, and the defensive reaction of living tissues with vascular systems to injury factors is called inflammation.

Bacteria, viruses, rickettsiae, mycoplasma, fungi, spirochetes, parasites, etc. are the most common causes of inflammation. At present, the traditional Chinese medicine for resisting bacteria, diminishing inflammation and resisting viruses has single type and small inhibiting effect on microorganisms. Therefore, the significance of obtaining the novel traditional Chinese medicine composition with the functions of antibiosis, antiphlogosis and antivirus is great.

Disclosure of Invention

In order to solve the technical problems, the invention provides an antibacterial, anti-inflammatory and antiviral traditional Chinese medicine composition, and a preparation method and application thereof.

The invention aims to provide a traditional Chinese medicine composition for resisting bacteria, diminishing inflammation and resisting viruses, which is prepared from the following components in parts by weight:

10-20 parts of honeysuckle, 2-8 parts of mint, 3-9 parts of safflower, 6-12 parts of hawthorn, 15-25 parts of bunge corydalis herb, 6-12 parts of tree peony bark, 3-9 parts of common monkshood mother root and 6-10 parts of eucommia bark.

Further, the traditional Chinese medicine composition is prepared from the following components in parts by weight: 15 parts of honeysuckle, 2 parts of mint, 3 parts of safflower, 6 parts of hawthorn, 15 parts of bunge corydalis herb, 6 parts of tree peony bark, 3 parts of common monkshood mother root and 6 parts of eucommia bark.

Further, the traditional Chinese medicine composition is prepared from the following components in parts by weight: 10 parts of honeysuckle, 4 parts of mint, 6 parts of safflower, 8 parts of hawthorn, 20 parts of bunge corydalis herb, 8 parts of tree peony bark, 6 parts of common monkshood mother root and 8 parts of eucommia bark.

Further, the traditional Chinese medicine composition is prepared from the following components in parts by weight: 20 parts of honeysuckle, 8 parts of mint, 3 parts of safflower, 6 parts of hawthorn, 25 parts of bunge corydalis herb, 12 parts of tree peony bark, 9 parts of common monkshood mother root and 10 parts of eucommia bark.

Further, the traditional Chinese medicine composition is prepared from the following components in parts by weight: 15 parts of honeysuckle, 6 parts of mint, 9 parts of safflower, 12 parts of hawthorn, 18 parts of bunge corydalis herb, 10 parts of tree peony bark, 4 parts of common monkshood mother root and 9 parts of eucommia bark.

The second purpose of the invention is to provide a preparation method of the traditional Chinese medicine composition, which comprises the following steps:

s1, weighing the honeysuckle, the mint, the safflower, the hawthorn, the bunge corydalis herb, the tree peony bark, the monkshood and the eucommia bark according to the weight parts, and drying for later use;

s2, pulverizing the Chinese medicinal materials weighed in S1 into fine powder, and mixing to obtain the Chinese medicinal composition.

Furthermore, the traditional Chinese medicine composition can be used for preparing pharmaceutically acceptable oral preparations.

The third purpose of the invention is to provide the application of the traditional Chinese medicine composition in preparing antibacterial, anti-inflammatory and antiviral medicines.

Compared with the prior art, the invention has the beneficial effects that:

1. the raw materials used by the traditional Chinese medicine composition have the following characteristics:

honeysuckle flower, flos Lonicerae has the effects of clearing away heat and toxic material, and cooling and dispelling wind-heat. Can be used for treating carbuncle, furuncle, pharyngitis, erysipelas, toxic heat, dysentery, wind-heat type common cold, epidemic febrile disease, and fever; has inhibitory effect on various bacteria (typhoid bacillus, paratyphoid bacillus, large intestine bacillus, proteus bacillus, pseudomonas aeruginosa, pertussis bacillus, cholera vibrio, staphylococcus, streptococcus, pneumococcus and meningococcus, etc.) in vitro; has strong effect on salmonella, particularly has strong inhibition on typhoid and paratyphoid bacilli in vitro; it can inhibit Shigella at high concentration, and has strong effect on Shigella shigella at low concentration, and Shigella shigella is inferior and Shigella freundii is almost ineffective. It is also said that the honeysuckle extract is effective against staphylococcus aureus, but not against escherichia coli. It also has antibacterial effect against some common bacteria (pneumococcus, Streptococcus A, and Catalpa) in chronic tracheitis (plate method).

Mint mainly contains volatile oil, levomenthol, isomenthone, pulegone, menthyl acetate, isopimfiil, peppermint isoflavacin, rosmarinic acid and other ingredients, and belongs to the category of exterior-releasing drugs.

Safflower containing 13 components of carthamin, neocarthamin, pre-carthamin, safflower quinonoid, nonacosane, beta-sitosterol, dipalmitoic acid, glyceride, oleic acid, linoleic acid, chlorogenic acid, etc.;

safflower yellow, which has the functions of slightly exciting the heart, reducing coronary resistance, increasing coronary flow and myocardial trophism blood flow; resisting myocardial ischemia, myocardial infarction and arrhythmia; improving peripheral microcirculation disturbance; anticoagulation; reducing blood fat; improving the hypoxia tolerance; exciting the uterus; relieving intestinal spasm; has immunological activity and anti-inflammatory effect; can relieve metabolic disorder of monoamine neuromediators in brain tissue; may be caused to change abruptly. Animal experiments show that small dosage of the decoction has an exciting effect on heart, and large dosage of the decoction has an inhibiting effect; has obvious direct contraction effect on blood vessels; the safflower yellow can obviously prolong the survival time of the left lung of the mouse in an anoxic environment; has obvious inhibiting effect on platelet aggregation caused by ADP; has analgesic effect.

Hawthorn fruit, mainly contains organic acids (citric acid and its methyl ester, malic acid, etc.) and flavonoids (hyperoside, quercetin, vitexin, etc.). In addition, it contains levo-epicatechin, quercetin, beta-sitosterol, citric acid, ursolic acid, linolenic acid, linoleic acid, gallic acid, protocatechuic acid, etc., and has effects of promoting digestion, protecting cardiovascular system, lowering blood pressure, reducing blood lipid, resisting oxidation, enhancing immunity, resisting bacteria, preventing cancer, contracting uterus, and relieving pain. The organic acid in the hawthorn is an effective component for promoting digestion and removing food stagnation, and the flavonoid compound is an active component for preventing and treating cardiovascular diseases and reducing blood fat; the hawthorn preparation has been loaded into pharmacopoeia in germany as a drug for the treatment of cardiovascular diseases, and condensed flavans (flavan polymers) are one of the quality standards thereof. The hawthorn flavone or hydrolysate has cardiotonic effect on in vitro or in vivo heart of Bufo siccus; the extract, flavone or hydrolysate can increase coronary blood flow; the total flavone, hydrolysate and triterpenic acid have blood pressure lowering effect; the extract, alcohol extract and total triterpenic acid extract can reduce blood lipid; in addition, it has effects of enhancing immunity, resisting oxidation, and resisting bacteria. Toxicity: total flavone mice were injected intraperitoneally with LDso at 165 mg/kg.

Dibutyl Cao, please heat and induce diuresis, remove toxicity and relieve swelling, treat furuncle, carbuncle, scrofula, jaundice, dysentery, diarrhea, conjunctival congestion, sore throat and venomous snake bite.

Cortex moutan, mainly contain paeonol neo-glycoside, benzoyl paeoniflorin, benzoyl oxidized paeoniflorin, gallic acid, etc., have central inhibitory action, improve the function of cardiovascular system, have apparent anticoagulation effects, anti-inflammatory, antibacterial, can strengthen the immunologic function of organism, have apparent inhibitory action to rat stress gastric ulcer and gastric fluid secretion, still have inhibitory action to ileum, uterus contraction, still have diuretic action, have apparent inhibitory action to the calcium channel blocker receptor in vitro;

radix Aconiti, containing aconitine, hypaconitine, mesaconine, lappaconitine, aconitine A, isottaladine, talamine, racemic demethyl eupatorine, uracil, aconitan A, B, C, D, dispelling pathogenic wind and removing dampness, warming channels and relieving pain. Has antiinflammatory, analgesic, blood sugar lowering, and anticancer effects.

Eucommia bark, containing pinoresinol diglucoside, D-eugenol, eucommia bark glucoside, eucommia bark element A, red dihydroxy dehydro-dibastinol, etc., is warm in nature and sweet in taste. Entering liver channel and kidney channel, and can be used for diminishing inflammation; has blood pressure lowering effect; enhancing the immune function of the organism; affecting pituitary-adrenal system function; has inhibitory effect on isolated uterus; has diuretic effect; has obvious analgesic and anti-inflammatory effects. Pharmacological experiments show that the water extract and the alcohol extract of the bark and the leaf have the functions of acute blood pressure reduction and blood vessel dilatation.

2. The preparation method of the traditional Chinese medicine composition is simple, the steps of water decoction, oil decoction and the like are not needed, the loss of effective components is avoided, and the bacteriostatic effect of the traditional Chinese medicine composition is optimal;

the traditional Chinese medicine components are mutually compatible to jointly inhibit staphylococcus aureus, colibacillus and beta hemolytic streptococcus to treat diseases (such as gynecological diseases) caused by the germs, in the traditional Chinese medicine composition, honeysuckle, bunge corydalis herb and tree peony bark are used as monarch drugs to play a role in inhibiting bacteria and resisting viruses, eucommia bark, hawthorn and monkshood are used as ministerial drugs to cooperate with the honeysuckle, the bunge corydalis herb and the tree peony bark to resist bacteria, the bacteriostatic spectrum of the medicine is increased, and the mint, the safflower and the monkshood are used as assistant drugs to cooperate with each other.

3. The traditional Chinese medicine composition prepared by the invention has strong inhibition on staphylococcus aureus, escherichia coli and beta hemolytic streptococcus, and can inhibit the growth of the microorganisms. Particularly, the inhibitor has the best inhibiting effect on staphylococcus aureus.

4. The traditional Chinese medicine composition prepared by the invention has the functions of resisting bacteria, diminishing inflammation and resisting viruses, and lays a theoretical foundation for treating gynecological inflammation.

Detailed Description

The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The experimental methods described in the examples of the present invention are all conventional methods unless otherwise specified.

Example 1:

the embodiment provides an antibacterial, anti-inflammatory and antiviral traditional Chinese medicine composition which is prepared from the following components:

15g of honeysuckle, 2g of mint, 3g of safflower, 6g of hawthorn, 15g of bunge corydalis herb, 6g of tree peony bark, 3g of Sichuan aconite root and 6g of eucommia bark.

The preparation process is simple: weighing the raw materials according to the mass, drying at 50-60 ℃, then crushing the raw materials into fine powder, and uniformly mixing to obtain the traditional Chinese medicine composition.

Example 2:

10g of honeysuckle, 4g of mint, 6g of safflower, 8g of hawthorn, 20g of bunge corydalis herb, 8g of tree peony bark, 6g of Sichuan aconite root and 8g of eucommia bark.

Example 3:

20g of honeysuckle, 8g of mint, 3g of safflower, 6g of hawthorn, 25g of bunge corydalis herb, 12g of tree peony bark, 9g of common monkshood mother root and 10g of eucommia bark.

Example 4:

15g of honeysuckle, 6g of mint, 9g of safflower, 12g of hawthorn, 18g of bunge corydalis herb, 10g of tree peony bark, 4g of Sichuan aconite root and 9g of eucommia bark.

The Chinese medicinal composition has the following contraindications in use: it is not suitable for pregnant women.

The effects of the Chinese medicinal compositions prepared in the above examples 1 to 4 are similar, and the bacteriostatic effect of the Chinese medicinal composition prepared in the invention is studied as represented by example 1. The specific process is as follows:

1. preparing the traditional Chinese medicine composition antibacterial liquid

The traditional Chinese medicine composition prepared in the example 1 is uniformly mixed with distilled water according to the proportion of 1g to 10mL to obtain the traditional Chinese medicine composition antibacterial liquid for later use.

2. Preparation of pathogenic bacteria liquid

Respectively obtaining a golden yellow staphylococcus culture solution, an escherichia coli culture solution, a B hemolytic streptococcus culture solution and a proteus culture solution by a conventional culture method;

inoculating Staphylococcus aureus to LB liquid medium, and culturing at 37 deg.C to OD₆₀₀When the value is equal to 1, the staphylococcus aureus culture solution is obtained; inoculating Escherichia coli into LB liquid medium, and culturing at 37 deg.C to OD₆₀₀Equal to 1, namely the Escherichia coli culture solution; inoculating hemolytic streptococcus BInoculating to liquid culture medium, and culturing to OD₆₀₀Equal to 1, the beta hemolytic streptococcus culture solution is obtained.

LB liquid medium: 10g of tryptone, 5g of yeast extract and 10g of NaCl.

Liquid medium for culturing beta hemolytic streptococcus:

18g of glucose, 10g of peptone, 10g of beef extract and MgSO₄·7H₂O 1g，KH₂PO₄2g, distilled water to a constant volume of 1L, pH 7.0.

2. Assessment of bacteriostatic Activity

Adding 2mL/100mL of Chinese medicinal composition antibacterial solution into the above Staphylococcus aureus culture solution, Escherichia coli culture solution, and beta hemolytic streptococcus culture solution, culturing, and detecting OD every 4 hr₆₀₀Record analysis. The results are shown in Table 1.

TABLE 1 bacteriostatic effect of the Chinese medicinal composition bacteriostatic liquid

OD₆₀₀	0h	4h	8h	12h	16h
						Culture solution of staphylococcus aureus	1.0	0.5	0.3	0.2	0.2
Escherichia coli culture solution	1.0	0.7	0.4	0.4	0.3
						Culture solution of beta hemolytic streptococcus	1.0	0.8	0.6	0.5	0.5

As can be seen from Table 1, the Chinese medicinal composition prepared by the invention has strong inhibition on staphylococcus aureus, escherichia coli and beta hemolytic streptococcus, and can inhibit the growth of the microorganisms. Particularly, the inhibitor has the best inhibiting effect on staphylococcus aureus. Therefore, the compound can be used for treating the disease infected by the pathogenic bacteria (such as gynecological inflammation).

While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the appended claims be interpreted as including the preferred embodiment and all such alterations and modifications as fall within the scope of the invention.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. The traditional Chinese medicine composition with the functions of resisting bacteria, diminishing inflammation and resisting viruses is characterized by being prepared from the following components in parts by weight: 10-20 parts of honeysuckle, 2-8 parts of mint, 3-9 parts of safflower, 6-12 parts of hawthorn, 15-25 parts of bunge corydalis herb, 6-12 parts of tree peony bark, 3-9 parts of common monkshood mother root and 6-10 parts of eucommia bark.

2. The traditional Chinese medicine composition according to claim 1, which is prepared from the following components in parts by weight: 15 parts of honeysuckle, 2 parts of mint, 3 parts of safflower, 6 parts of hawthorn, 15 parts of bunge corydalis herb, 6 parts of tree peony bark, 3 parts of common monkshood mother root and 6 parts of eucommia bark.

3. The traditional Chinese medicine composition according to claim 1, which is prepared from the following components in parts by weight: 10 parts of honeysuckle, 4 parts of mint, 6 parts of safflower, 8 parts of hawthorn, 20 parts of bunge corydalis herb, 8 parts of tree peony bark, 6 parts of common monkshood mother root and 8 parts of eucommia bark.

4. The traditional Chinese medicine composition according to claim 1, which is prepared from the following components in parts by weight: 20 parts of honeysuckle, 8 parts of mint, 3 parts of safflower, 6 parts of hawthorn, 25 parts of bunge corydalis herb, 12 parts of tree peony bark, 9 parts of common monkshood mother root and 10 parts of eucommia bark.

5. The traditional Chinese medicine composition according to claim 1, which is prepared from the following components in parts by weight: 15 parts of honeysuckle, 6 parts of mint, 9 parts of safflower, 12 parts of hawthorn, 18 parts of bunge corydalis herb, 10 parts of tree peony bark, 4 parts of common monkshood mother root and 9 parts of eucommia bark.

6. A method for preparing the Chinese medicinal composition of any one of claims 1 to 5, comprising the steps of:

7. The preparation method of claim 6, wherein the Chinese medicinal composition can be used for preparing a pharmaceutically acceptable oral preparation.

8. An application of the Chinese medicinal composition of claim 1 in preparing the medicines for resisting bacteria, diminishing inflammation and resisting viruses.