CN107582691B - Compound phellodendron bark liquid embrocation gargle gel - Google Patents
Compound phellodendron bark liquid embrocation gargle gel Download PDFInfo
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Abstract
The invention provides compound cortex phellodendri liquid masking liquid mouthwash gel which is prepared from the following raw materials in percentage by weight: 0.1-10% of chitosan, 0.1-2% of carbomer, 2-40% of glycerol, 0.05-0.1% of ethylparaben, 0.5-4.0% of triethanolamine, 2-10% of propylene glycol, and 2-5% of tween-800.5, the balance being a medicinal extract of compound phellodendron bark liquid; the medicine extracting solution of the compound phellodendron amurense liquid is prepared by concentrating the mixed solution of the plant medicine extracting solution and the centipede enzymatic hydrolysate at low temperature; the gel matrix is preliminarily determined according to the physicochemical property of the effective components of the compound phellodendron bark liquid. Has the functions of inhibiting various bacteria, curing the ulcer surface into a film, effectively isolating pathogenic bacteria, resisting inflammation, relieving pain and promoting healing, and has good curative effect on oral ulcer caused by radiotherapy and chemotherapy.
Description
Technical Field
The invention relates to the field of medicinal preparations, in particular to compound cortex phellodendri liquid liniment mouthwash gel, and preparation and application thereof.
Background
According to the statistics of the world health organization, the 5-year survival rate of the current malignant tumor treatment is 45 percent, radiotherapy and chemotherapy are one of the main treatment methods of the current malignant tumor, and 60 to 70 percent of patients take radiotherapy and chemotherapy as the first choice treatment or the component of comprehensive treatment. Radiotherapy and chemotherapy also play a significant role in the treatment of oral, maxillofacial tumors. However, while the radiotherapy and chemotherapy drugs kill tumor cells and improve the cure rate of malignant tumors, the radiotherapy and chemotherapy drugs can also cause damage to normal tissues, so that chemotherapy oral ulcer is caused, the morbidity of the radiotherapy and chemotherapy drugs can reach more than 60%, the life quality of patients is seriously affected, and the further treatment of the patients is hindered.
Chemotherapy oral ulcer mostly occurs 5 days after chemotherapy, is one of the most common complications of malignant tumor chemotherapy, and can cause oral mucosa erythema, erosion, ulcer, local pain, enlargement of submandibular lymph nodes, and a few patients also have general symptoms such as fever, systemic hypodynamia, poor appetite and the like. The oral mucosa nerve endings are rich, the pain is obvious after the ulcer happens, the pain is particularly aggravated when the oral mucosa nerve endings take food and water, and the multiple pain causes multiple pains on bodies and spirits of patients. The compound phellodendron gel mouthwash mainly comprises carbomer, chitosan, fructus forsythiae, honeysuckle, phellodendron and the like, and forms an adhesive sealing film after being applied to ulcer parts of oral mucosa to cover ulcer surfaces, and the film shields the stimulation of external physical factors and chemical factors on ulcer-damaged mucosal tissues, so that the compound phellodendron gel mouthwash has the effects of quickly curing the oral ulcer, relieving ulcer pain and improving eating conditions.
The adjuvants for preparing gel mouthwash mainly comprise poloxamer, chitosan, carbomer, etc. However, the traditional Chinese medicine extract has complex components and is often acted with matrix auxiliary materials, or the traditional Chinese medicine extract is a hypertonic system, so that water in a gel matrix network structure can be infiltrated outwards, and the gel structure is irreversibly damaged.
Disclosure of Invention
In order to overcome the problems, the invention provides compound cortex phellodendri liquid masking mouthwash gel, and a gel matrix is preliminarily determined according to the physicochemical property dosage form of the effective components of the compound cortex phellodendri liquid. The results show that: the compound phellodendron amurense liquid liniment mouthwash gel has an inhibition effect on various bacteria, can be solidified into a layer of film on the surface of an ulcer, is effectively isolated from pathogenic bacteria, has the effects of resisting inflammation, relieving pain and promoting healing, and has a good curative effect on oral ulcer caused by radiotherapy and chemotherapy.
In order to achieve the purpose, the invention adopts the following technical scheme:
a compound phellodendron bark liquid embrocation gargle gel is composed of the following raw materials by weight percent: 0.1-10% of chitosan, 0.1-2% of carbomer, 2-40% of glycerol, 0.05-0.1% of ethylparaben, 0.5-4.0% of triethanolamine, 2-10% of propylene glycol, and 2-5% of tween-800.5, the balance being a medicinal extract of compound phellodendron bark liquid;
the medicine extracting solution of the compound phellodendron amurense liquid is prepared by concentrating the mixed solution of the plant medicine extracting solution and the centipede enzymatic hydrolysate at low temperature; wherein the plant medicinal material extract comprises water extract and alcohol extract of fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci; the Scolopendra enzymolysis solution comprises pepsin extract and trypsin extract of Scolopendra.
The aqueous extract and the alcohol extract of forsythia, phellodendron, honeysuckle and dandelion, and the pepsin extract and the trypsin extract of centipede can be extracted according to the conventional method in the field.
Preferably, the compound phellodendron amurense liquid masking agent mouthwash gel is prepared from the following raw materials in percentage by weight: 0.1-5% of chitosan, 0.1-1% of carbomer, 2-20% of glycerol, 0.05-0.75% of ethylparaben, 0.5-2.0% of triethanolamine, 2-6% of propylene glycol, 2.5% of tween-800.5 and the balance of a medicinal extract of compound phellodendron bark liquid;
the medicine extracting solution of the compound phellodendron amurense liquid is prepared by concentrating the mixed solution of the plant medicine extracting solution and the centipede enzymatic hydrolysate at low temperature; wherein the plant medicinal material extract comprises water extract and alcohol extract of fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci; the Scolopendra enzymolysis solution comprises pepsin extract and trypsin extract of Scolopendra.
Preferably, the compound phellodendron amurense liquid masking agent mouthwash gel is prepared from the following raw materials in percentage by weight: 5-10% of chitosan, 1-2% of carbomer, 20-40% of glycerol, 0.75-0.1% of ethylparaben, 2.0-4.0% of triethanolamine, 6-10% of propylene glycol, 802.5-5% of tween-tween and the balance of a medicinal extract of compound phellodendron bark liquid;
the medicine extracting solution of the compound phellodendron amurense liquid is prepared by concentrating the mixed solution of the plant medicine extracting solution and the centipede enzymatic hydrolysate at low temperature; wherein the plant medicinal material extract comprises water extract and alcohol extract of fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci; the Scolopendra enzymolysis solution comprises pepsin extract and trypsin extract of Scolopendra.
The invention also provides compound cortex phellodendri liquid masking liquid mouthwash gel which is prepared from the following raw materials in percentage by weight: poloxamer 40718-25%, poloxamer 1884-10%, glycerol 5-15%, propylene glycol 5-15%, ethylparaben 0.05-2%, and the rest is medicinal extractive solution of compound cortex Phellodendri solution;
when the ethylparaben and the propylene glycol are used together, the water solubility of the ethylparaben and the propylene glycol can be effectively increased, but a complex can be formed to influence the bacteriostatic action of the ethylparaben and the propylene glycol. For this reason, the invention optimizes the dosage of the propylene glycol and the ethylparaben, and finds that: in the medicine extracting solution of the compound phellodendron amurense liquid, the mass ratio of propylene glycol to ethylparaben is 1-3: 0.05-2, the compatibility of the three is best, the film is easy to form, and the antiseptic and antibacterial properties of the ethylparaben are best.
The medicine extracting solution of the compound phellodendron amurense liquid is prepared by concentrating the mixed solution of the plant medicine extracting solution and the centipede enzymatic hydrolysate at low temperature; wherein the plant medicinal material extract comprises water extract and alcohol extract of fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci; the Scolopendra enzymolysis solution comprises pepsin extract and trypsin extract of Scolopendra.
Preferably, the compound phellodendron amurense liquid masking agent mouthwash gel is prepared from the following raw materials in percentage by weight: poloxamer 40718-22%, poloxamer 1884-7%, glycerol 5-10%, propylene glycol 5-10%, ethylparaben 0.05-1%, and the rest is medicinal extractive solution of compound cortex Phellodendri solution;
the medicine extracting solution of the compound phellodendron amurense liquid is prepared by concentrating the mixed solution of the plant medicine extracting solution and the centipede enzymatic hydrolysate at low temperature; wherein the plant medicinal material extract comprises water extract and alcohol extract of fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci; the Scolopendra enzymolysis solution comprises pepsin extract and trypsin extract of Scolopendra.
Preferably, the compound phellodendron amurense liquid masking agent mouthwash gel is prepared from the following raw materials in percentage by weight: poloxamer 40722-25%, poloxamer 1887-10%, glycerol 10-15%, propylene glycol 10-15%, ethylparaben 1-2%, and the rest is medicinal extractive solution of compound cortex Phellodendri solution;
the medicine extracting solution of the compound phellodendron amurense liquid is prepared by concentrating the mixed solution of the plant medicine extracting solution and the centipede enzymatic hydrolysate at low temperature; wherein the plant medicinal material extract comprises water extract and alcohol extract of fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci; the Scolopendra enzymolysis solution comprises pepsin extract and trypsin extract of Scolopendra.
Preferably, the preparation method of the medicine extract of the compound phellodendron amurense liquid is as follows:
taking fructus forsythiae, cortex phellodendri, honeysuckle and dandelion as raw materials, extracting with alcohol, concentrating under reduced pressure, adding water for dilution, and refrigerating to obtain an alcohol extract;
decocting the above medicinal residue, concentrating under reduced pressure, diluting with alcohol solution, refrigerating, filtering, collecting supernatant, and concentrating under pressure to obtain water extractive solution;
mixing the above alcoholic extractive solution and water extractive solution to obtain plant medicinal material extractive solution;
processing Scolopendra fine powder and artificial gastric juice in water bath at 37 deg.C for 30min under sealed condition;
adding pepsin into the treated substance for enzymolysis to obtain an enzymolysis liquid;
treating the enzymatic hydrolysate in water bath at 85 deg.C for 15min, cooling, centrifuging, and collecting supernatant to obtain pepsin extractive solution;
drying the residue after extraction with pepsin, adding artificial intestinal juice, and bathing at 53 deg.C for 30min under sealed condition;
adding trypsin into the residue mixed solution after the water bath treatment for enzymolysis,
treating the enzymolysis solution at 85 deg.C in water bath for 15min, cooling, centrifuging, and collecting supernatant to obtain trypsin extract;
mixing the above pepsin extract and trypsin extract to obtain Scolopendra enzymolysis solution;
mixing the above plant material extractive solution and Scolopendra enzymolysis solution, and concentrating at low temperature to obtain medicinal extractive solution.
The invention also provides a preparation method of the better medicine extract of the compound phellodendron bark liquid, which comprises the following steps:
1.1 prescription
80 plus or minus 10g of fructus forsythiae, 40 plus or minus 5g of golden cypress, 40 plus or minus 5g of honeysuckle, 40 plus or minus 5g of dandelion and 2.4 plus or minus 0.5g of centipede
1.2 extraction and purification process
1.2.1 plant medicinal material extract
(1) Taking fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci according to the prescription amount, adding 6 times of 70% ethanol, and performing ultrasonic extraction for 2-3 times;
(2) concentrating the alcohol extract under reduced pressure (55 ℃) until the relative density is 1.00-1.05 (50 ℃);
(3) adding distilled water to 3-4 times of the crude drug amount, stirring, and refrigerating for 12 hr to obtain ethanol extractive solution;
(4) adding 6 times of water into the residue after alcohol extraction, and decocting and extracting for 2 times, 1h each time;
(5) concentrating the water extract under reduced pressure (75 deg.C) to relative density of 1.10-1.15(50 deg.C);
(6) adding 95% ethanol to ethanol content of 50%, stirring, and refrigerating for 24 hr;
(7) filtering, collecting supernatant, and concentrating under reduced pressure until no alcohol smell is present to obtain water extractive solution;
(8) mixing the ethanol extractive solution and water extractive solution, and mixing to obtain plant medicinal material extractive solution;
1.2.2 Scolopendra enzymatic hydrolysate
(1) Taking centipede fine powder with the prescription amount into a 100ml round-bottom flask with a stopper, adding 50ml artificial gastric juice, sealing, and acting in 37 ℃ constant-temperature water bath for 30 min;
(2) adding pepsin (3.0% of enzyme-substrate ratio) for enzymolysis for 3 h;
(3) placing the enzymolysis solution in 85 deg.C constant temperature water bath for 15min, cooling with running water to room temperature, centrifuging at 3000r/min for 15min, and collecting supernatant;
(4) vacuum drying the residue obtained by the best extraction process of pepsin at 60 deg.C, weighing, placing in 100ml round-bottom flask with plug, adding 25ml artificial intestinal juice, sealing, and acting in 53 deg.C constant temperature water bath for 30 min;
(5) adding a certain amount of trypsin (6.0% of enzyme-substrate ratio) for enzymolysis for 4h, placing the enzymolysis solution in a constant temperature water bath at 85 ℃ for 15min, cooling the enzymolysis solution to room temperature with running water, centrifuging at 3000r/min for 15min, and taking supernatant to obtain the product.
Mixing the pepsin extract and the trypsin extract to obtain the centipede enzymatic hydrolysate.
1.2.3 pharmaceutical extracts
Mixing the plant medicinal material extract with Scolopendra enzymolysis solution, and concentrating at low temperature to obtain medicinal extract.
The invention also provides a preparation method of the compound phellodendron amurense liquid liniment mouthwash gel, which comprises the following steps:
uniformly spraying carbomer on the water surface according to the prescription amount to fully swell the carbomer;
taking chitosan according to the prescription amount, adding purified water for soaking, and dropwise adding glacial acetic acid for dissolving;
adding glycerol, propylene glycol, tween-80 and chitosan solution into the fully swollen carbomer;
adding triethanolamine to adjust pH, and adding the medicinal extractive solution of any one of the above cortex Phellodendri solutions and ethylparaben under magnetic stirring.
The invention also provides a preparation method of the compound phellodendron amurense liquid liniment mouthwash gel, which comprises the following steps:
slowly and uniformly spreading the prescription amounts of P407 and P188 on the water surface, and then placing the mixture in a refrigerator at 4 ℃ for refrigeration for 24 hours to slowly and fully swell the mixture to obtain a clear, lump-free and uniformly dispersed solution;
adding the medicinal extract of the ethyl hydroxybenzoate solution and the compound phellodendron bark solution with the prescription amount under the condition of magnetic stirring.
The compound phellodendron amurense liquid liniment has ideal effect on treating chemotherapy oral ulcer.
The prescribed amount in the present invention is an amount prescribed in any one of the above-mentioned formulations of the present invention.
The invention has the beneficial effects that:
1. the invention primarily determines the gel matrix according to the physicochemical property dosage form of the effective component of the compound phellodendron bark liquid. The traditional Chinese medicine composition has an inhibiting effect on various bacteria, can be solidified into a layer of film on the surface of the ulcer, is effectively isolated from pathogenic bacteria, has the functions of resisting inflammation, relieving pain and promoting healing, and has a good curative effect on oral ulcer caused by radiotherapy and chemotherapy.
2. The polymer material used in the preparation is biodegradable, and has no toxic or side effect.
3. The research proves that: the compound phellodendron amurense liquid coating agent has reasonable formula, feasible preparation process, strong specificity of a quality control method, good reproducibility and good economic and social benefits.
4. The preparation method is simple, convenient to operate and high in practicability. Can contain the extract of the Chinese herbal compound, has mild technological conditions and is convenient for clinical popularization and application; is convenient to carry and use by patients.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1
First, research on process of medicinal material extract
1.1 prescription
80 plus or minus 10g of fructus forsythiae, 40 plus or minus 5g of golden cypress, 40 plus or minus 5g of honeysuckle, 40 plus or minus 5g of dandelion and 2.4 plus or minus 0.5g of centipede
1.2 extraction and purification process
1.2.1 plant medicinal material extract
(1) Taking fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci according to the prescription amount, adding 6 times of 70% ethanol, and performing ultrasonic extraction for 2-3 times;
(2) concentrating the alcohol extract under reduced pressure (55 ℃) until the relative density is 1.00-1.05 (50 ℃);
(3) adding distilled water to 3-4 times of the crude drug amount, stirring, and refrigerating for 12 hr to obtain ethanol extractive solution;
(4) adding 6 times of water into the residue after alcohol extraction, and decocting and extracting for 2 times, 1h each time;
(5) concentrating the water extract under reduced pressure (75 deg.C) to relative density of 1.10-1.15(50 deg.C);
(6) adding 95% ethanol to ethanol content of 50%, stirring, and refrigerating for 24 hr;
(7) filtering, collecting supernatant, and concentrating under reduced pressure until no alcohol smell is present to obtain water extractive solution;
(8) mixing the ethanol extractive solution and water extractive solution, and mixing to obtain plant medicinal material extractive solution;
1.2.2 Scolopendra enzymatic hydrolysate
(1) Taking centipede fine powder with the prescription amount into a 100ml round-bottom flask with a stopper, adding 50ml artificial gastric juice, sealing, and acting in 37 ℃ constant-temperature water bath for 30 min;
(2) adding pepsin (3.0% of enzyme-substrate ratio) for enzymolysis for 3 h;
(3) placing the enzymolysis solution in 85 deg.C constant temperature water bath for 15min, cooling with running water to room temperature, centrifuging at 3000r/min for 15min, and collecting supernatant;
(4) vacuum drying the residue obtained by the best extraction process of pepsin at 60 deg.C, weighing, placing in 100ml round-bottom flask with plug, adding 25ml artificial intestinal juice, sealing, and acting in 53 deg.C constant temperature water bath for 30 min;
(5) adding a certain amount of trypsin (6.0% of enzyme-substrate ratio) for enzymolysis for 4h, placing the enzymolysis solution in a constant temperature water bath at 85 ℃ for 15min, cooling the enzymolysis solution to room temperature with running water, centrifuging at 3000r/min for 15min, and taking supernatant to obtain the product.
Mixing the pepsin extract and the trypsin extract to obtain the centipede enzymatic hydrolysate.
1.2.3 pharmaceutical extracts
Mixing the plant medicinal material extract with Scolopendra enzymolysis solution, and concentrating at low temperature to obtain medicinal extract.
Second, research on forming process
Prescription: 0.1-10% of chitosan, 0.1-2% of carbomer, 2-40% of glycerol, 0.05-0.1% of ethylparaben, 0.5-4.0% of triethanolamine, 2-10% of propylene glycol, and 2-5% of tween-800.5, and the balance of the medicinal extract of the compound phellodendron bark liquid.
(1) Uniformly spraying carbomer on the water surface according to the prescription amount to fully swell the carbomer;
(2) taking chitosan according to the prescription amount, adding purified water for soaking, and dropwise adding glacial acetic acid for dissolving;
(3) adding glycerol, propylene glycol, tween-80 and chitosan solution into the fully swollen carbomer;
(4) adding triethanolamine to adjust pH, and adding the medicinal material extract and ethylparaben under magnetic stirring.
Example 2
First, research on process of medicinal material extract
1.1 prescription
80 plus or minus 10g of fructus forsythiae, 40 plus or minus 5g of golden cypress, 40 plus or minus 5g of honeysuckle, 40 plus or minus 5g of dandelion and 2.4 plus or minus 0.5g of centipede
1.2 extraction and purification process
1.2.1 plant medicinal material extract
(1) Taking fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci according to the prescription amount, adding 6 times of 70% ethanol, and performing ultrasonic extraction for 2-3 times;
(2) concentrating the alcohol extract under reduced pressure (55 ℃) until the relative density is 1.00-1.05 (50 ℃);
(3) adding distilled water to 3-4 times of the crude drug amount, stirring, and refrigerating for 12 hr to obtain ethanol extractive solution;
(4) adding 6 times of water into the residue after alcohol extraction, and decocting and extracting for 2 times, 1h each time;
(5) concentrating the water extract under reduced pressure (75 deg.C) to relative density of 1.10-1.15(50 deg.C);
(6) adding 95% ethanol to ethanol content of 50%, stirring, and refrigerating for 24 hr;
(7) filtering, collecting supernatant, and concentrating under reduced pressure until no alcohol smell is present to obtain water extractive solution;
(8) mixing the ethanol extractive solution and water extractive solution, and mixing to obtain plant medicinal material extractive solution;
1.2.2 Scolopendra enzymatic hydrolysate
(1) Taking centipede fine powder with the prescription amount into a 100ml round-bottom flask with a stopper, adding 50m1 artificial gastric juice, sealing, and acting in 37 ℃ constant-temperature water bath for 30 min;
(2) adding pepsin (3.0% of enzyme-substrate ratio) for enzymolysis for 3 h;
(3) placing the enzymolysis solution in 85 deg.C constant temperature water bath for 15rmin, cooling to room temperature with running water, centrifuging at 3000r/min for 15min, and collecting supernatant;
(4) vacuum drying the residue obtained by the best extraction process of pepsin at 60 deg.C, weighing, placing in 100ml round-bottomed flask with plug, adding 25m1 artificial intestinal juice, sealing, and acting in 53 deg.C constant temperature water bath for 30 min;
(5) adding a certain amount of trypsin (6.0% of enzyme-substrate ratio) for enzymolysis for 4h, placing the enzymolysis solution in a constant temperature water bath at 85 ℃ for 15min, cooling the enzymolysis solution to room temperature with running water, centrifuging at 3000r/min for 15min, and taking supernatant to obtain the product.
Mixing the pepsin extract and the trypsin extract to obtain the centipede enzymatic hydrolysate.
1.2.3 pharmaceutical extracts
Mixing the plant medicinal material extract with Scolopendra enzymolysis solution, and concentrating at low temperature to obtain medicinal extract.
Second, research on forming process
Prescription: poloxamer 40718-25%, poloxamer 1884-10%, glycerol 5-15%, propylene glycol 5-15%, ethylparaben 0.05-2%, and the rest is medicinal extractive solution of compound cortex Phellodendri solution.
(1) The prescription amounts of P407 and P188 are slowly and uniformly sprinkled on the water surface, and then the water surface is refrigerated for 24 hours in a refrigerator at 4 ℃ to slowly and fully swell the mixture, so that clear, non-agglomeration and uniformly dispersed solution is obtained.
(2) Adding the ethylparaben solution and the medicine extract according to the prescription amount under the condition of magnetic stirring to obtain the medicine.
Example 3 analgesic and antibacterial assays
Firstly, an analgesic test:
1. test method
Mice of Kunming species were used, and were randomly divided into 3 groups: a model group, a compound phellodendron bark liquid group (namely, a compound phellodendron bark liquid sold in the market), and a compound phellodendron bark liquid liniment gargle gel group. Each group had 5 mice.
Each group of mice was given the corresponding drug at a dose set of 10ml/kg, 9 a.m. each day: 00-10: 00, 3 pm: 00-4: 00 each administration is carried out for 2 times, 3 days continuously, 30min after the last administration, 0.8% acetic acid (0.1ml/10g) is injected into the abdominal cavity, and the writhing frequency of the mice is observed after each group of mice are injected with acetic acid for 15 min.
2. Test results
Compared with a blank control group, the compound cortex phellodendri liquid group and the compound cortex phellodendri liquid liniment gargle gel group have the advantages that the mouse twisting times are obviously reduced, and the statistical difference is obvious.
TABLE 1 influence of gargle gel of compound cortex Phellodendri liquid liniment on the times of mouse writhing (x + -s)
Note: p <0.05 compared to control group
The results show that: the compound phellodendron amurense liquid liniment mouthwash gel in the embodiment 1 has a remarkable analgesic effect.
II, antibacterial test:
[ Experimental methods ]
MIC assay
1) Preparation of a bacterial culture medium: MH (B) Broth was purchased from Oboxing, Beijing, and was autoclaved at 121 ℃ for 20min for use after preparation as described.
2) Preparing experimental bacteria liquid: the concentration of the suspension was adjusted to 1X 106CFU/ml using a turbiditube.
3) Micro-dilution method bacteriostasis experiment: the reference to NCCLS standard was made by microdilution. Adding 100 mu l of culture medium into each well of a 96-well plate, then adding 100 mu l of liquid medicine into the row 1, sucking 100 mu l of mixed liquid from the row 1, adding the mixed liquid into the row 2, and repeating the steps until the row 6, thereby obtaining the diluted medicine gradient of 2 times, 4 times, 8 times, 16 times, 32 times and 64 times. And 10 mul of gentamicin is added into each hole of the 7 th row to serve as a positive control, and bacterial liquid is directly added into the 8 th row to serve as a negative control. Drug dilution gradients and media blanks were also set as controls. Each drug was repeated twice. The culture medium was added to 10. mu.l of each culture medium at 37 ℃ for 24 hours, and the results were observed.
2. Test results
Comparative example 1
The preparation method and the raw materials are the same as example 2, except that the medicinal extract of the compound phellodendron bark liquid of the present invention is replaced by the commercially available compound phellodendron bark liquid.
The results show that: the film formation is difficult and the antibacterial property is lowered.
It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the present invention is not limited thereto, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and equivalents can be made in the technical solutions described in the foregoing embodiments, or equivalents thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. Although the present invention has been described with reference to the specific embodiments, it should be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.
Claims (10)
1. The compound phellodendron amurense liquid masking agent mouthwash gel is characterized by comprising the following raw materials in percentage by weight: 0.1-10% of chitosan, 0.1-2% of carbomer, 2-40% of glycerol, 0.05-0.1% of ethylparaben, 0.5-4.0% of triethanolamine, 2-10% of propylene glycol, and 2-5% of tween-800.5, the balance being a medicinal extract of compound phellodendron bark liquid;
the medicine extracting solution of the compound phellodendron amurense liquid is prepared by concentrating the mixed solution of the plant medicine extracting solution and the centipede enzymatic hydrolysate at low temperature; wherein the plant medicinal material extract comprises water extract and alcohol extract of fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci; the centipede enzymatic hydrolysate consists of centipede pepsin extract and trypsin extract, and the compound phellodendron bark liquid comprises the following raw materials: 80 plus or minus 10g of fructus forsythiae, 40 plus or minus 5g of golden cypress, 40 plus or minus 5g of honeysuckle, 40 plus or minus 5g of dandelion and 2.4 plus or minus 0.5g of centipede.
2. The compound phellodendron amurense liquid embrocation gargle gel as claimed in claim 1, which is prepared from the following raw materials in percentage by weight: 0.1-5% of chitosan, 0.1-1% of carbomer, 2-20% of glycerol, 0.05-0.075% of ethylparaben, 0.5-2.0% of triethanolamine, 2-6% of propylene glycol, 2.5% of tween-800.5 and the balance of a medicine extracting solution of compound phellodendron bark solution.
3. The compound phellodendron amurense liquid embrocation gargle gel as claimed in claim 1, which is prepared from the following raw materials in percentage by weight: 5-10% of chitosan, 1-2% of carbomer, 20-40% of glycerol, 0.075-0.1% of ethylparaben, 2.0-4.0% of triethanolamine, 6-10% of propylene glycol, 802.5% -5% of tween-tween and the balance of a medicinal extract of compound phellodendron bark liquid.
4. The compound phellodendron amurense liquid masking agent mouthwash gel is characterized by comprising the following raw materials in percentage by weight: poloxamer 40718-25%, poloxamer 1884-10%, glycerol 5-15%, propylene glycol 5-15%, ethylparaben 0.05-2%, and the rest is medicinal extractive solution of compound cortex Phellodendri solution;
the medicine extracting solution of the compound phellodendron amurense liquid is prepared by concentrating the mixed solution of the plant medicine extracting solution and the centipede enzymatic hydrolysate at low temperature; wherein the plant medicinal material extract comprises water extract and alcohol extract of fructus forsythiae, cortex Phellodendri, flos Lonicerae and herba Taraxaci; the centipede enzymatic hydrolysate consists of centipede pepsin extract and trypsin extract, and the compound phellodendron bark liquid comprises the following raw materials: 80 plus or minus 10g of fructus forsythiae, 40 plus or minus 5g of golden cypress, 40 plus or minus 5g of honeysuckle, 40 plus or minus 5g of dandelion and 2.4 plus or minus 0.5g of centipede.
5. The compound cortex phellodendri liquid embrocation mouthwash gel according to claim 4, which is prepared from the following raw materials in percentage by weight: poloxamer 40718-22%, poloxamer 1884-7%, glycerol 5-10%, propylene glycol 5-10%, ethylparaben 0.05-1%, and the rest is medicinal extractive solution of compound cortex Phellodendri solution.
6. The compound cortex phellodendri liquid embrocation mouthwash gel according to claim 4, which is prepared from the following raw materials in percentage by weight: poloxamer 40722-25%, poloxamer 1887-10%, glycerol 10-15%, propylene glycol 10-15%, ethylparaben 1-2%, and the rest is medicinal extractive solution of compound cortex Phellodendri solution.
7. The compound cortex phellodendri liquid embrocation mouthwash gel according to any one of claims 1 to 6, wherein the preparation method of the medicine extract of the compound cortex phellodendri liquid is as follows:
taking fructus forsythiae, cortex phellodendri, honeysuckle and dandelion as raw materials, extracting with alcohol, concentrating under reduced pressure, adding water for dilution, and refrigerating to obtain an alcohol extract;
decocting the above medicinal residue, concentrating under reduced pressure, diluting with alcohol solution, refrigerating, filtering, collecting supernatant, and concentrating under pressure to obtain water extractive solution;
mixing the above alcoholic extractive solution and water extractive solution to obtain plant medicinal material extractive solution;
processing Scolopendra fine powder and artificial gastric juice in water bath at 37 deg.C for 30min under sealed condition;
adding pepsin into the treated substance for enzymolysis to obtain an enzymolysis liquid;
treating the enzymatic hydrolysate in water bath at 85 deg.C for 15min, cooling, centrifuging, and collecting supernatant to obtain pepsin extractive solution;
drying the residue after extraction with pepsin, adding artificial intestinal juice, and bathing at 53 deg.C for 30min under sealed condition;
adding trypsin into the residue mixed solution after the water bath treatment for enzymolysis,
treating the enzymolysis solution at 85 deg.C in water bath for 15min, cooling, centrifuging, and collecting supernatant to obtain trypsin extract;
mixing the above pepsin extract and trypsin extract to obtain Scolopendra enzymolysis solution;
mixing the above plant material extractive solution and Scolopendra enzymolysis solution, and concentrating at low temperature to obtain medicinal extractive solution.
8. The preparation method of the compound phellodendron amurense liquid embrocation gargle gel of any one of claims 1 to 3, which is characterized by comprising the following steps:
uniformly spreading carbomer on water surface to make it fully swell;
soaking chitosan in purified water, and dropwise adding glacial acetic acid for dissolving;
adding glycerol, propylene glycol, tween-80 and chitosan solution into the fully swollen carbomer;
adding triethanolamine to adjust pH, and adding the medicinal extractive solution of cortex Phellodendri solution and ethylparaben under magnetic stirring.
9. The preparation method of the compound phellodendron amurense liquid embrocation gargle gel of any one of claims 4 to 6, which is characterized by comprising the following steps:
slowly and uniformly spreading poloxamer 407 and poloxamer 188 on the water surface, and then placing the mixture in a refrigerator at 4 ℃ for refrigeration for 24 hours to slowly and fully swell the mixture to obtain a clear, lump-free and uniformly dispersed solution;
adding the medicinal extractive solution of ethylparaben solution and compound cortex Phellodendri solution under magnetic stirring.
10. Use of the compound cortex phellodendri liquid embrocation mouthwash gel of any one of claims 1 to 7 in the preparation of a medicament for treating chemotherapy-induced oral ulcers.
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| CN113289026B (en) * | 2021-06-29 | 2022-03-15 | 山东汉方制药有限公司 | Compound cortex phellodendri composition, compound cortex phellodendri spray preparation, preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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-
2016
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Non-Patent Citations (3)
| Title |
|---|
| "复方黄柏温敏凝胶的制备和连翘苷含量测定";刘珊珊等;《药学服务与研究》;20150831;第15卷(第4期);第269页左栏第1段、右栏第1段、第270页左栏第1、4段 * |
| 健康问答;萌宇;《https://ask.360kad.com/question/1923204.html》;20160606;第1页"满意回答"部分 * |
| 刘珊珊等."复方黄柏温敏凝胶的制备和连翘苷含量测定".《药学服务与研究》.2015,第15卷(第4期),269-272. * |
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