CN112471180A

CN112471180A - Traditional Chinese medicine disinfectant and preparation method thereof

Info

Publication number: CN112471180A
Application number: CN202011602881.6A
Authority: CN
Inventors: 张文悦
Original assignee: Harbin Unike Technology Development Co ltd
Current assignee: Harbin Unike Technology Development Co ltd
Priority date: 2020-12-30
Filing date: 2020-12-30
Publication date: 2021-03-12

Abstract

The invention relates to a traditional Chinese medicine disinfectant and a preparation method thereof, and the active ingredients of the disinfectant are prepared from the following raw materials: 5-20 parts of scutellaria baicalensis, coptis chinensis, golden cypress, rheum officinale, dandelion, isatis root, polygonum cuspidatum, houttuynia cordata, Chinese violet, honeysuckle, fructus forsythiae, purslane and mint; the traditional Chinese medicine disinfectant disclosed by the invention is strong in sterilizing capability, high in acting speed, strong in stability, free of stimulation to human skin, easy to dissolve in water, free of residue after use, safe, free of toxic and side effects, capable of being widely applied to disinfection in daily life, public places and the like, and is an ideal disinfectant worth being popularized.

Description

Traditional Chinese medicine disinfectant and preparation method thereof

Technical Field

The invention relates to the field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine disinfectant and a preparation method thereof.

Background

In daily life, bacteria and viruses are inevitably contacted. These bacteria and viruses are sometimes pathogenic and some are nonpathogenic. For today where new coronaviruses exist, it is essential to ensure health every day. It is also necessary to eliminate a series of bacteria and pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Proteus vulgaris, etc.

The disinfectant containing chlorine, phosphorus and benzene is used too much, so that the disinfectant has strong allergenicity, and the traditional Chinese medicine is used for preparing the low-sensitivity broad-spectrum disinfectant by exploiting the classic of the traditional Chinese medicine on the premise of clear pharmacology and has good market prospect.

Disclosure of Invention

The traditional Chinese medicine disinfectant has the advantages of strong sterilization capability, high action speed, strong stability, no stimulation to human skin, easy water dissolution, no residue after use, safety, no toxic or side effect, wide application in disinfection of daily life, public places and the like, and is an ideal disinfectant worth popularization.

In order to achieve the above purpose, the technical scheme of the invention is implemented as follows:

the traditional Chinese medicine disinfectant comprises the following raw materials for preparing active ingredients of the disinfectant in parts by weight:

36-52 parts of scutellaria baicalensis, 36-52 parts of coptis chinensis, 35-50 parts of golden cypress, 35-50 parts of rheum officinale

30-52 parts of dandelion, 26-4 parts of isatis root, 26-47 parts of giant knotweed rhizome, 26-47 parts of heartleaf houttuynia herb

22-39 parts of Chinese violet, 22-39 parts of honeysuckle, 20-39 parts of forsythia, 16-35 parts of purslane

5-20 parts of mint

The effective components of the disinfectant are prepared from the following raw materials in parts by weight:

scutellaria root 48 parts coptis root 48 parts phellodendron 46 parts rhubarb

47 parts of dandelion, 42 parts of isatis root, 42 parts of giant knotweed, 42 parts of cordate houttuynia

34 parts of Chinese violet, 34 parts of honeysuckle, 33 parts of forsythia, 30 parts of purslane

Mint 12 parts

baikal skullcap root 44 parts coptis root 44 parts phellodendron bark 42 parts rhubarb 42 parts

Dandelion 40 parts of isatis root 36 parts of giant knotweed 36 parts of cordate houttuynia 36 parts

30 parts of Chinese violet, 30 parts of honeysuckle, 28 parts of forsythia, 25 parts of purslane

Mint 15 parts

40 parts of scutellaria root, 40 parts of coptis root, 38 parts of phellodendron bark, 38 parts of rhubarb

33 parts of dandelion, 30 parts of radix isatidis, 30 parts of polygonum cuspidatum and 30 parts of houttuynia cordata

26 parts of Chinese violet, 26 parts of honeysuckle, 23 parts of forsythia, 20 parts of purslane

Mint 10 parts

A preparation method of a traditional Chinese medicine disinfectant comprises the following process steps:

(1) removing impurities from Scutellariae radix, Coptidis rhizoma, cortex Phellodendri, radix et rhizoma Rhei, herba Taraxaci, radix Isatidis, rhizoma Polygoni Cuspidati, herba Houttuyniae, herba Violae, flos Lonicerae, fructus forsythiae, and herba Portulacae, cleaning, placing into a container, adding 8-10 times of distilled water, soaking for 3-5 hr, boiling for 1-2 hr, and extracting; adding 5-7 times of distilled water again, boiling for 2-3 hr, and extracting; finally, adding 2-4 times of distilled water, heating and boiling for 1-2 hours, and extracting; mixing the three extractive solutions, and filtering to obtain filtrate;

(2) concentrating the filtrate to a liquor ratio of 1:2-1:4, adding 30-80% ethanol, precipitating with ethanol for 24-48 hr

(3) And carrying out solid-liquid separation on the liquid to obtain supernatant, heating to 50-80 ℃, adding a certain amount of mint, fully stirring and filtering to prepare the traditional Chinese medicine disinfectant.

The traditional Chinese medicine used by the invention has the following medicinal properties:

scutellaria baicalensis: bitter taste and cold nature. It enters lung, gallbladder, spleen, large intestine and small intestine meridians. Clear heat and dry dampness, purge fire and remove toxicity, stop bleeding, prevent abortion. Can be used for treating damp-warm syndrome, summer-heat dampness, chest distress, emesis, dampness and heat distention, dysentery, jaundice, cough due to lung heat, hyperpyrexia, polydipsia, hematemesis, carbuncle, swelling, sore, and threatened abortion. Modern pharmacological research proves that the scutellaria has the function of resisting pathogenic microorganisms. Research proves that the scutellaria has broad-spectrum antibacterial action and has inhibition effect on various gram-positive bacteria and gram-negative bacteria, particularly staphylococcus aureus and pseudomonas aeruginosa, and also has certain inhibition effect on various pathogenic fungi such as dermatophyte. The effective bacteriostatic component is baicalin. The Scutellariae radix decoction and water decoction have inhibitory effect on influenza A virus PR6 strain and Asian A type, and can be used for treating mice infected with influenza virus in vitro. In vitro experiments also show that the scutellaria has the functions of inhibiting the growth of amebiasis and killing leptospira.

Coptis chinensis: bitter in taste, cold in nature, entering heart, liver, stomach and large intestine meridians. Clear heat and dry dampness, purge fire and remove toxicity. Can be used for treating damp-heat distention, fullness, emesis, acid regurgitation, dysentery, jaundice, hyperpyrexia, coma, excessive heart-fire, vexation, insomnia, hematemesis, hemorrhage, conjunctival congestion, toothache, diabetes, carbuncle, furuncle, etc.; it is used externally to treat eczema, eczema and purulent ear canal. The New plains of materia Medica: huang Lian is bitter and cold in taste, can ascend and descend, and is also nontoxic. Enter the heart and the cell collaterals. It can enter liver when purging fire. Herbs that are about the same as meridians can enter heart, especially meridians. Vomiting stopping, acid regurgitation relieving, thirst quenching. For severe fire eyes, it can calm heart, stop dream-related, relieve mania, relieve stuffiness and fullness, and relieve swelling of women's vulva. For infantile malnutrition due to soil-eating disorder, it has special actions of resolving summer-heat, damp-heat and stagnated heat. "

Phellodendron bark: bitter taste and cold nature; it enters kidney, bladder and large intestine meridians. Clearing away heat. Dry dampness, purge fire, remove toxicity. Treating dysentery due to heat, diarrhea, diabetes, jaundice, flaccidity, 36484m, nocturnal emission, stranguria with turbid urine, hemorrhoid, hematochezia, leucorrhea, hectic fever due to yin-steaming, conjunctival congestion, sore of the mouth and tongue, and pyocutaneous disease and toxic swelling. The herbal decoction (Ben Cao Tang Ye): huang Bai, Zu Shao Yin Ji, kidney bitter and dry, so it is also indicated for kidney damp-retention, Zhi Zi and Huang Qin enter lung, Huang Lian enters heart, Huang Bai Zhi enters kidney and dry dampness enters, and they are also classified from each other. "

Rhubarb: bitter and cold. It enters spleen, stomach, large intestine, liver and pericardium meridians. Purging the pathogenic accumulation, clearing heat and purging fire, cooling blood and removing toxicity, removing blood stasis and dredging channels. Modern pharmacology has demonstrated that: the rhubarb has the anti-infection effect and has the inhibition effect on various gram positive and negative bacteria, wherein the most sensitive bacteria are staphylococcus and streptococcus, and the most sensitive bacteria are diphtheria bacillus, typhoid bacillus, paratyphoid bacillus, pneumococcus, dysentery bacillus and the like; it also has inhibitory effect on influenza virus.

And (4) dandelion: cold in nature, bitter and sweet in taste. It enters liver meridian and stomach meridian. Clear heat and remove toxicity, induce diuresis and dissipate nodulation. Belongs to heat-clearing and toxicity-removing herbs classified under heat-clearing herbs. The Yunnan herbal medicine: application for sore and pyogenic infections, scabies, tinea and sore; dispelling pathogenic wind, eliminating sore toxin, and dispersing scrofula and tuberculosis; it can be used for treating hematochezia, stranguria, dysuria and urinary bladder. "

Radix isatidis: bitter and cold in flavor. Clear heat and remove toxicity, cool blood and relieve sore throat. The book materia Medica is just written: the radix isatidis, i.e. indigowoad root, has the same functional taste as indigowoad leaf, can enter the blood system of liver and stomach, and has no excessive effects of clearing heat, detoxifying, avoiding epidemic diseases and killing insects. But leaves are scattered and roots are descending, which are also different from ears. "

Giant knotweed rhizome: slightly bitter taste and slightly cold nature; it enters liver, gallbladder and lung meridians. Promoting diuresis and removing jaundice, clearing heat and detoxicating, promoting blood circulation and removing blood stasis, eliminating phlegm and relieving cough, and relaxing bowels with purgative. Modern medical research proves that the giant knotweed rhizome decoction (20 percent) has the inhibition effect on staphylococcus aureus, staphylococcus albus, hemolytic streptococcus, catacoccus, escherichia coli, proteus, shigella flexneri, pseudomonas aeruginosa and the like in vitro and has the killing effect on leptospira.

Houttuynia cordata: 2, pungent taste; slightly cold in nature; it enters lung meridian. Clear heat and remove toxicity, induce diuresis and alleviate edema. Treating pneumonia, lung abscess, dysentery, malaria, edema, gonorrhea, leucorrhea, carbuncle, swelling, hemorrhoid, rectocele, eczema, alopecia, scabies and tinea. Houttuynia cordata, which is a traditional Chinese medicine, is well known. The houttuynia cordata is a natural and safe antibiotic and can clear heat, diminish inflammation and resist viruses. The houttuynia cordata is used as the plant antibiotic, and the most rarely obtained property of the houttuynia cordata can reach the upper, middle and lower triple energizers of a human body. It can treat pharyngitis, pneumonia, urethritis, vaginitis, nephritis, inflammation and herpes on skin. The houttuynia cordata is also widely applied to infections caused by various bacteria and viruses, such as wind-heat type cold, influenza, urinary system infection, reproductive system infection and the like.

Herba Violae: cold in nature, bitter and pungent in flavor. It enters heart and liver meridians. Clearing away heat and toxic material, cooling and relieving swelling. Belonging to the category of heat-clearing herbs. Modern pharmacological studies have shown that: the whole grass has the function of resisting pathogenic microorganisms. A high molecular compound with high activity for resisting I-type AIDS virus in vitro test is separated from the whole grass, and the sulfonated polysaccharide has the molecular weight of 10000-15000. Has antibacterial, cholagogic, diuretic and laxative effects; has inhibitory effect on Staphylococcus aureus, Streptococcus pneumoniae, and Diplococcus pneumoniae.

Honeysuckle flower: cold in nature and sweet in taste. It enters lung meridian, heart meridian and stomach meridian. Clearing away heat and toxic material, and cooling and dispelling wind-heat. Belonging to the category of heat-clearing herbs. In vitro experiments show that the honeysuckle decoction and the alcohol immersion liquid have certain inhibition effects on various gram positive and negative bacteria such as staphylococcus aureus, staphylococcus albus, hemolytic streptococcus, pneumonia bacillus, meningococcus, typhoid bacillus, paratyphoid bacillus, escherichia coli, dysentery bacillus, proteus bacillus, bordetella pertussis, pseudomonas aeruginosa, mycobacterium tuberculosis, vibrio cholerae and the like. The decoction, water extract and purified liquid of honeysuckle flower have good killing and inhibiting effects on the streptococcus mutans causing dental caries by using a plate punching method, and the bacteriostatic effect is obviously enhanced along with the increase of the concentration.

Fructus forsythiae: slightly cold in nature and bitter in taste. It enters lung meridian, heart meridian and small intestine meridian. Clearing away heat and toxic material, relieving swelling and dissipating stagnation. Belonging to the category of heat-clearing herbs. It can be used for treating dysentery, heart and abdomen psychroalgia, and pyocutaneous disease. It is indicated for abscess, scrofula, acute mastitis, erysipelas, wind-heat type common cold, early epidemic febrile disease, high fever, polydipsia, coma, macula, stranguria and anuresis.

Purslane: sour and cold in taste; it enters liver and large intestine meridians. Has the effects of clearing away heat and toxic materials, cooling blood, stopping bleeding, and relieving dysentery. It is mainly used for treating heat-toxin bloody dysentery, heat-toxin sores and ulcers, metrorrhagia and metrostaxis, and hematochezia.

Mint: pungent taste and cool nature. Enter lung and liver meridians. Disperse wind-heat, clear heat and improve eyesight, relieve sore throat and promote eruption, sooth liver and move qi. Can be used for treating wind-heat common cold, headache, sore throat, food stagnation, flatulence, aphtha, toothache, scabies, urticaria, early stage of epidemic febrile disease, rubella pruritus, stagnation of qi due to depression of the liver, chest distress, and hypochondriac pain.

Compared with the prior art, the invention has the following beneficial effects:

the traditional Chinese medicine disinfectant disclosed by the invention is strong in sterilizing capability, high in acting speed, strong in stability, free of stimulation to human skin, easy to dissolve in water, safe, free of toxic and side effects, capable of being widely applied to disinfection in daily life and public places, and is an ideal disinfectant which is worthy of popularization.

Detailed Description

The invention is further illustrated with reference to specific examples.

Example 1

The traditional Chinese medicine disinfectant comprises the following active ingredients in parts by weight:

5-20 parts of mint

The preparation method comprises the following process steps:

Example 2

scutellaria root 48 parts coptis root 48 parts phellodendron 46 parts rhubarb

Mint 12 parts

The preparation method and the using method of the traditional Chinese medicine disinfectant are the same as those in the embodiment 1.

Example 3

Mint 15 parts

Example 4

Mint 10 parts

The following bacteriostatic experiments were carried out on the disinfectant of the present invention:

1. experimental strains:

selecting representative strains in a laboratory, such as staphylococcus aureus, candida albicans, pseudomonas aeruginosa, escherichia coli and bacillus subtilis.

2. And (3) strain culture medium:

nutrient agar culture medium, nutrient broth culture medium, Sabouraud's dextrose agar culture medium and Sabouraud's dextrose liquid culture medium.

3. And (4) experimental classification:

the bacteriostasis experiment comprises an experiment group and a control group, wherein the experiment group adopts the traditional Chinese medicine disinfectant, and the control group adopts hypochlorous acid disinfectant.

4. The specific experimental steps are as follows:

(1) preparation of bacterial liquid

Respectively inoculating staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and bacillus subtilis strains on nutrient agar culture media, culturing for 36 hours at 37 ℃, respectively selecting typical colonies from the agar culture media, respectively inoculating the typical colonies into fresh nutrient broth culture media, and culturing for 24 hours at the same temperature for later use. The Candida albicans is inoculated on a Sabouraud's dextrose agar culture medium, cultured for 48-72 hours at 25 ℃, typical colonies are selected from the agar culture medium and respectively inoculated in a fresh Sabouraud's dextrose liquid culture medium, and cultured for 24 hours at the same temperature for standby.

(2) Selection of disinfectant

45ml of the traditional Chinese medicine disinfectant and 45ml of the hypochlorous acid disinfectant are respectively put into a dilution container, the two dilution containers are respectively diluted by 30 times, and the two dilution solutions are respectively put into three containers and are put under the aseptic condition for standby.

(3) Strain split charging and marking

The experimental strains cultured in the nutrient broth in the step (1) for 24 hours are respectively diluted by 50 times by using sterile nutrient broth, each strain is respectively filled in two culture dishes, and a control group and an experimental group are respectively marked. The experimental strains cultured for 24 hours in the Sabouraud's dextrose liquid culture medium are respectively diluted by 50 times by using the sterile Sabouraud's dextrose liquid culture medium, each strain is respectively arranged in two culture dishes, and a control group and an experimental group are respectively marked.

(4) Sterilization

And (3) respectively dripping the diluted solution of the traditional Chinese medicine disinfectant and the diluted solution of the hypochlorous acid disinfectant obtained in the step (2) into a culture dish of an experimental group and a culture dish of a control group.

5. Test observation and measurement:

and observing the three groups of strains, measuring the residual areas of the strains, and recording the observation data result.

6. And after observation and measurement are finished, placing the culture dishes under an aseptic condition, standing for two days, and after the disinfectant is completely volatilized, tracking and observing to find that colonies of the control group continue to reproduce and colonies of the experimental group do not change.

7. The experimental results are as follows:

the sterilizing capability of the traditional Chinese medicine disinfectant in the experimental group is far greater than that of the disinfectant in the control group, and the continuous propagation of strains can be inhibited.

Claims

1. A traditional Chinese medicine disinfectant is characterized in that: the effective components of the disinfectant are prepared from the following raw materials in parts by weight:

5-20 parts of mint.

2. The traditional Chinese medicine disinfectant as claimed in claim 1, wherein: the effective components of the disinfectant are prepared from the following raw materials in parts by weight:

scutellaria root 48 parts coptis root 48 parts phellodendron 46 parts rhubarb

12 parts of mint.

3. The traditional Chinese medicine disinfectant as claimed in claim 1, wherein: the effective components of the disinfectant are prepared from the following raw materials in parts by weight:

And 15 parts of mint.

4. The traditional Chinese medicine disinfectant as claimed in claim 1, wherein: the effective components of the disinfectant are prepared from the following raw materials in parts by weight:

10 parts of mint.

5. The method for preparing the traditional Chinese medicine disinfectant as claimed in any one of claims 1 to 4, which is characterized in that: the method comprises the following process steps:

(2) concentrating the filtrate to a liquor ratio of 1:2-1:4, adding 30-80% ethanol, and precipitating for 24-48 h;