CN103768298A - Disinfectant for clinical laboratory and preparation method thereof - Google Patents

Disinfectant for clinical laboratory and preparation method thereof Download PDF

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CN103768298A
CN103768298A CN201410050757.1A CN201410050757A CN103768298A CN 103768298 A CN103768298 A CN 103768298A CN 201410050757 A CN201410050757 A CN 201410050757A CN 103768298 A CN103768298 A CN 103768298A
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herba
rhizoma
disinfectant solution
clinical laboratory
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CN103768298B (en
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徐春娥
杨金艳
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Zhang Dongmei
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Abstract

The invention relates to a disinfectant for a clinical laboratory and a preparation method thereof. The disinfectant disclosed by the invention is prepared from the following effective components: radix angelicae, sticktight, sanguisorba officinalis, poinsettia, cimicifugae foetidae, caesalpinia sepiaria, euphorbia helioscopia, hemp fimble stem-fibre, arcangelisia loureiri, Cineraria repanda, hoodshaped alocasia rhizome, wormwood, cyrtomium fortune, utricularia bifida, anemone vitifolia and puberulous glochidion herb. The medicinal materials selected in the disinfectant disclosed by the invention are compatible; the disinfectant disclosed by the invention is prepared by utilizing the modern advanced pharmaceutical technology and has the advantages of being broad-spectrum for disinfection, rapid in action speed, strong in stability and the like; the disinfectant disclosed by the invention can be widely applied to disinfecting skin of people in the clinical laboratory and appliances and is free from irritation to human-body skin, free from residue after being used, easy to dissolve in water, and safe and free from toxic and side effects; the working efficiency of people in the clinical laboratory is improved; the accurate rate of the inspection result is ensured; the disinfectant disclosed by the invention is ideal disinfectant worthy of being popularized.

Description

One is disinfectant solution and preparation method for clinical laboratory
Technical field
The present invention relates to the field of Chinese medicines, particularly disinfectant solution and preparation method for a kind of clinical laboratory.
Background technology
Clinical laboratory is the bridge between clinical medicine and preclinical medicine, comprises the subdisciplines such as clinical chemistry, clinical microbiology, clinical immunology, hematology, osology and transfusion science.Clinical laboratory bears and comprise ward, door emergency case, all kinds of health check-up and the various human bodies of scientific research and the testing of zoological specimens every day.
When medical institutions carry out medical test, the bacterial flora system of checking system should minimize, this trier that just skin to medical personnel and inspection institute need carries out omnibearing sterilization thoroughly, eliminate a series of antibacterial and pathogen etc., for example staphylococcus aureus, escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, proteus vulgaris etc.
, when the personnel of clinical laboratory test, must carry out disinfection to the both hands of oneself, the normal method adopting is to be immersed in disinfectant solution at present for this reason, and existing disinfectant solution is mainly ethanol, hydrogen peroxide, and iodine tincture, potassium permanganate, also has glutaraldehyde, povidone iodine etc.These disinfectant solution are mostly chemicals, have strong impulse, and often use can make medical personnel's skin be subject to serious stimulation, and some irritated people's watch face can become dry and crowfoot cracks, and dermatitis and eczema even can occur.And reusing of disinfectant solution developed immunity to drugs antibacterial etc., have a strong impact on Disinfection Effect, not only increase reviewer's work difficulty, also affect the result of check.
For this reason, find a kind of safe, effectively and the disinfectant solution having no side effect significant for the every check work of clinical laboratory.
Summary of the invention
The invention provides a kind of clinical laboratory disinfectant solution and preparation method, the selected medical material compatibility of disinfectant solution of the present invention is suitable and utilize modern advanced medical pharmaceutical technology to make, there is sterilization wide spectrum, the advantage such as speed of action is fast, stability is strong, can be widely used in the personnel of clinical laboratory skin and sterilization of instruments, non-stimulated to human skin, by rear noresidue, soluble in water, safe without toxic side effect, improve the personnel's of clinical laboratory work efficiency, having guaranteed the accuracy rate of assay, is a kind of desirable disinfectant solution being worthy to be popularized.
For achieving the above object, technical scheme of the present invention is implemented as follows:
A kind of clinical laboratory disinfectant solution, raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
Radix Angelicae Dahuricae 60-90 part Herba Bidentis Bipinnatae 60-90 part Radix Sanguisorbae 55-85 part poinsettia 55-85 part
Rhizoma Cimicifugae 55-85 part Semen Caesalpiniae 50-80 part Herba Euphorbiae Helioscopiae 50-80 part Cortex Cannabis 48-75 part
Caulis Arcangelisiae 48-75 part Herba Senecionis Scandentis 48-75 part Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) 40-66 part Herba Swertiae davidi 40-66 part
Rhizoma Osmundae 32-55 part Herba Carpesii Cernui 32-55 part Herba anemones vitifoliae 26-48 part Fructus glochidionis puberi(Herba Glochidii Puberi) 26-48 part.
Raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
77 parts of 77 portions of poinsettia of 80 portions of Radix Sanguisorbaes of 80 portions of Herba Bidentis Bipinnatae of the Radix Angelicae Dahuricae
68 parts of 70 parts of Cortex Cannabis of 70 parts of Herba Euphorbiae Helioscopiae of 77 parts of Semen Caesalpiniae of Rhizoma Cimicifugae
58 parts of 58 parts of Herba Swertiae davidis of 68 parts of Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) of 68 parts of Herba Senecionis Scandentiss of Caulis Arcangelisiae
41 parts of 41 parts of Fructus glochidionis puberi(Herba Glochidii Puberi)s of 49 parts of Herba anemones vitifoliaes of 49 parts of Herba Carpesii Cernui of Rhizoma Osmundae.
Raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
70 parts of 70 portions of poinsettia of 72 portions of Radix Sanguisorbaes of 72 portions of Herba Bidentis Bipinnatae of the Radix Angelicae Dahuricae
62 parts of 63 parts of Cortex Cannabis of 63 parts of Herba Euphorbiae Helioscopiae of 70 parts of Semen Caesalpiniae of Rhizoma Cimicifugae
52 parts of 52 parts of Herba Swertiae davidis of 62 parts of Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) of 62 parts of Herba Senecionis Scandentiss of Caulis Arcangelisiae
36 parts of 36 parts of Fructus glochidionis puberi(Herba Glochidii Puberi)s of 43 parts of Herba anemones vitifoliaes of 43 parts of Herba Carpesii Cernui of Rhizoma Osmundae.
Raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
62 parts of 62 portions of poinsettia of 65 portions of Radix Sanguisorbaes of 65 portions of Herba Bidentis Bipinnatae of the Radix Angelicae Dahuricae
55 parts of 56 parts of Cortex Cannabis of 56 parts of Herba Euphorbiae Helioscopiae of 62 parts of Semen Caesalpiniae of Rhizoma Cimicifugae
46 parts of 46 parts of Herba Swertiae davidis of 55 parts of Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) of 55 parts of Herba Senecionis Scandentiss of Caulis Arcangelisiae
31 parts of 31 parts of Fructus glochidionis puberi(Herba Glochidii Puberi)s of 38 parts of Herba anemones vitifoliaes of 38 parts of Herba Carpesii Cernui of Rhizoma Osmundae.
A preparation method for disinfectant solution for clinical laboratory, comprises following processing step:
(1) get the Radix Angelicae Dahuricae, Rhizoma Cimicifugae, Cortex Cannabis and Rhizoma Osmundae and remove impurity, dry, be placed in mortar and grind into powder, cross 200 order fine powders for subsequent use;
(2) get Herba Bidentis Bipinnatae, Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) and Herba Carpesii Cernui and smash strand juice to pieces, filter, stay juice for subsequent use;
(3) get Radix Sanguisorbae, Semen Caesalpiniae, Herba Euphorbiae Helioscopiae, Caulis Arcangelisiae, Herba Senecionis Scandentis, Herba Swertiae davidi, Herba anemones vitifoliae and Fructus glochidionis puberi(Herba Glochidii Puberi) and put into container, add distilled water to boil and extract 3 times, add first 10-15 and doubly measure distilled water immersion 4-5 hour, heated and boiled 3-4 hour, extracts; Add for the second time 8-10 and doubly measure distilled water heated and boiled 2-3 hour, extract; Add for the third time 6-8 and doubly measure distilled water heated and boiled 1~2 hour, extract; Merge three times extracting solution, filter, obtain filtrate, for subsequent use;
(4) get poinsettia and dry in the sun, slow fire parch, dries in the air cool, grinds into powder, crosses 120 order fine powders, for subsequent use;
(5) above-mentioned steps (1)-(4) prepared fine powder, juice and filtrate are mixed, add assistant medicament, utilize modern advanced pharmacy science and technology, make disinfectant solution, be described clinical laboratory's disinfectant solution.
The property of medicine of the present invention's Chinese medicine used is as follows:
The Radix Angelicae Dahuricae: acrid in the mouth, warm in nature; Attach to the lung and stomach meridians.Expelling wind and cold, understand things pain-stopping, detumescence and apocenosis, dampness leukorrhagia stopping.Profit blood vessels, expelling wind and cold, eliminating dampness and dredging channels, is widely used in the treatment of the diseases such as soft tissue contusion, hyperosteogeny, muscular strain, rheumatic myositis, fracture, costal chondritis, scapulohumeral periarthritis, intercostal neuralgia.The Radix Angelicae Dahuricae all has inhibitory action to escherichia coli, dysentery bacterium, Bacillus proteus, Bacillus typhi, Salmonella paratyphi, bacillus pyocyaneus, vibrio cholera, Bacillus tuberculosis etc.
Herba Bidentis Bipinnatae: bitter in the mouth, cold nature.Heat-clearing and toxic substances removing, expelling wind and removing dampness, promoting blood circulation and detumescence.Cure mainly malaria, diarrhoea, dysentery, hepatitis, acute nephritis, stomachache, dysphagic, acute appendicitis, laryngopharynx swelling and pain, traumatic injury, snake bite and insect sting.Modern medicine study confirmation, the ethanol immersion of Herba Bidentis Bipinnatae has bacteriostasis to gram-positive bacterium in vitro, and flower, stem also have bacteriostasis to staphylococcus aureus.
Radix Sanguisorbae: bitter in the mouth, acid, cold in nature, nontoxic; Enter liver, lung, kidney, large intestine channel.Cooling blood for hemostasis, heat-clearing and toxic substances removing, detumescence sore.Cure mainly haematemesis, spitting of blood, epistaxis, hematuria, has blood in stool, hemorrhoidal bleeding, dysentery, metrorrhagia, sore, carbuncle and painful swelling, eczema, pudendal pruritus." not Lu " carries: " only pus and blood, all flaccidity, malignant boil, the wine that disappears, except quenching one's thirst, mends wound absolutely, and in puerperal, plug, can make incised wound cream, and main internal hemorrhage due to trauma is more than, insufficiency of blood." modern medicine study confirmation, Radix Sanguisorbae contains a large amount of tannins, has stronger convergence, energy vasoconstrictive, and the permeability of reduction blood vessel, reduces inflammatory exudation, also has obvious bactericidal action simultaneously, can resist charrin's disease.
Poinsettia: bitter in the mouth, cool in nature.Heat-clearing and toxic substances removing, dissipating blood stasis for subsidence of swelling.Cure mainly upper respiratory tract infection, oral ulcer, pneumonia, mastitis, enteritis, bacillary dysentery, urinary tract infection, furuncle carbuncle, eczema, traumatic injury, heat clearing away." the practical Chinese herbal medicine in Guangxi newly selects " carries: " anti-inflammation.Control acute upper respiratory tract inflammation, pneumonia, tonsillitis, oral ulcer, mastitis, chronic pelvic inflammatory disease, dysentery, diarrhoea, venom.”
Rhizoma Cimicifugae: sweet in the mouth, pungent, micro-hardship, cool in nature; Enter lung, spleen, stomach warp.Heat-clearing and toxic substances removing, delivers rash, ascending up spleen-Qi and Yang.Cure mainly epidemic disease fire-toxin, aphtha, pharyngalgia, macule, headache cold and heat, carbuncle sore tumefacting virus, sinking of QI of middle-JIAO, diarrhea due to hypofunction of the spleen, heavy under chronic dysentery, women's leukorrhagia, metrorrhagia.Modern medicine study confirmation, Rhizoma Cimicifugae can suppress the growth of tubercule bacillus in vitro, and the water logging agent of its mutation RHIIZOMA CIMICIFUGAE has inhibitory action in various degree to dermatophytess such as oidium schoenleiniis in vitro.
Semen Caesalpiniae: acrid in the mouth, hardship, warm in nature; Return lung, large intestine channel.Removing toxic substances dehumidifying, relieving cough and resolving phlegm, parasite killing.Main dysentery, malaria, chronic tracheitis, infantile malnutrition, malnutrition due to parasitic infestation." herbal classic " carries: " master lets out dysentery intestinal Bleach, the evil that uncharms stagnation of QI, and pain relieving, except cold and heat." modern medicine study confirmation, Semen Caesalpiniae decocting liquid has bacteriostasis to staphylococcus aureus.
Herba Euphorbiae Helioscopiae: acrid in the mouth, hardship, cool in nature, poisonous; Enter large and small intestinal, spleen channel.The expectorant of row water, parasite killing removing toxic substances.Cure mainly edematous asthma, phlegm retention is breathed with cough, malaria, bacillary dysentery, scrofula, tinea skin ulcer, Tuberculous flaccidity pipe, osteomyelitis.Essentials of Matea Medica carries: " cough-relieving, parasite killing." modern medicine study confirmation, when Herba Euphorbiae Helioscopiae high concentration decoct, can suppress the growth of tubercule bacillus.
Cortex Cannabis: bitter in the mouth, pungent, property is flat, poisonous.Preventing the attack (or recurrence) of malaria, drives ascarid, Dingchuan.Main malaria, ascariasis, asthma.Modern medicine study confirmation, Cortex Cannabis has antibacterial action.
Caulis Arcangelisiae: bitter in the mouth, cold in nature; GUIXIN, lung, large intestine channel.Clearing away heat-damp and promoting diuresis, removing toxic substances pain relieving.Be used for preventing influenza, leptospirosis, control acute gastroenteritis, bacillary dysentery, tonsillitis, bronchitis, malaria; Eye conjunctivitis is controlled in external, skin eczema, impetigo, vaginitis.Guangzhou army " conventional Chinese herbal medicine handbook " carries: " heat-clearing and toxic substances removing.Control enteritis, bacillary dysentery, tonsillitis, bronchitis, furuncle and phyma, malaria, dermatitis, eczema, impetigo, tinea pedis infects.”
Herba Senecionis Scandentis: bitter in the mouth, pungent, cold in nature.Heat-clearing and toxic substances removing, improving acuity of vision and removing nebula, killing parasites for relieving itching.Main flow sense, upper respiratory tract infection, pneumonia, acute tonsillitis, rib adenitis, acute enteritis, bacillary dysentery, icterohepatitis, gallbladder exudative dermatitis inflammation, acute urinary tract infection, conjunctival congestion and swelling pain cataracta, carbuncle furuncle poison, erysipelas, eczema, dry exudative dermatitis skin ulcer, trichomonal vaginitis, burn and scald.Modern medicine study confirmation, Herba Senecionis Scandentis decoct has stronger antibacterial action to dysentery bacterium and staphylococcus aureus.
Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae): acrid in the mouth, micro-hardship, cold in nature, poison greatly; Return lung meridian.Heat-clearing and toxic substances removing, removing obstruction for relieving pain.Cure mainly influenza, leptospirosis, skin infection pain poison from the beginning of, scrofula, cellulitis, chronic osteomyelitis, poisonous snake flood dragon wound, malicious peak sting.
Herba Swertiae davidi: bitter in the mouth, cool in nature.Clearing away lung-heat, parasite killing.For jaundice due to damp-heat, larynx redness, malignant boil scabies." Chinese herbal medicine is commonly used in Sichuan " carries: " clearing away lung-heat, parasite killing.The subcutaneous ulcer of harnessing the Yellow River, larynx redness, malignant boil scabies.”
Rhizoma Osmundae: bitter in the mouth, puckery, cold nature, slightly poisonous; Return liver, stomach warp.Parasite killing, heat-clearing and toxic substances removing, cooling blood for hemostasis.Cure mainly anemopyretic cold, warm skin eruption, haematemesis hemoptysis, epistaxis is had blood in stool, metrorrhagia dysentery, leukorrhagia." Shaanxi Chinese herbal medicine " carries: " heat-clearing and toxic substances removing, hemostasis parasite killing.The infectious disease such as prevention epidemic encephalitis type B, mumps.Treating hematochezia, hematuria, epistaxis, menorrhagia, ascariasis, pinworm disease.”
Herba Carpesii Cernui: bitter in the mouth, pungent, cold in nature, slightly poisonous.Heat-clearing and toxic substances removing, reducing swelling and alleviating pain.For cold, fever, laryngopharynx swelling and pain, toothache, acute enteritis, dysentery, urinary tract infection, tuberculous lymphadenitis; Furuncle toxic swelling, mastitis, parotitis, herpes zoster, venom are controlled in external.
Herba anemones vitifoliae: bitter in the mouth, pungent is poisonous; Return lung, liver, gallbladder meridian.Clearing away damp-heat, removing toxic substances parasite killing, the dissipating blood stasis of regulating the flow of vital energy.Cure mainly dysentery, jaundice malaria, ascariasis, knows parasitosis, infantile malnutrition, beriberi swells and ache, rheumatic ostalgia, traumatic injury, swollen ulcer drug, centipete bite.
Fructus glochidionis puberi(Herba Glochidii Puberi): bitter in the mouth, cool in nature, slightly poisonous; Return kidney channel.Removing damp-heat, resolving toxin and disinhibiting the throat, promoting flow of QI and blood.Cure mainly dysentery, have loose bowels, jaundice, malaria, stranguria with turbid discharge, leukorrhagia, laryngopharynx swelling and pain, toothache, colic, puerperal abdonimal pain.Modern medicine study confirmation, Folium glochidionis puberi decoct has inhibitory action to 9 kinds of intestinal such as shigella, paratyphoid bacillus A and Salmonella typhis, wherein the most responsive to congratulating dysentery bacterium.
The present invention compared with prior art, has following beneficial effect:
The selected medical material compatibility of disinfectant solution of the present invention is suitable and utilize modern advanced medical pharmaceutical technology to make, there is sterilization wide spectrum, the advantage such as speed of action is fast, stability is strong, can be widely used in the personnel of clinical laboratory skin and sterilization of instruments, non-stimulated to human skin, by rear noresidue, soluble in water, safe without toxic side effect, having improved the personnel's of clinical laboratory work efficiency, guaranteed the accuracy rate of assay, is a kind of desirable disinfectant solution being worthy to be popularized.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated.
Embodiment 1
Raw material composition and the weight of disinfectant solution effective ingredient of the present invention are:
Radix Angelicae Dahuricae 87g Herba Bidentis Bipinnatae 87g Radix Sanguisorbae 82g poinsettia 82g
Rhizoma Cimicifugae 82g Semen Caesalpiniae 77g Herba Euphorbiae Helioscopiae 77g Cortex Cannabis 74g
Caulis Arcangelisiae 74g Herba Senecionis Scandentis 74g Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) 64g Herba Swertiae davidi 64g
Rhizoma Osmundae 54g Herba Carpesii Cernui 54g Herba anemones vitifoliae 46g Fructus glochidionis puberi(Herba Glochidii Puberi) 46g.
The preparation method of disinfectant solution of the present invention, comprises following processing step:
(1) get the Radix Angelicae Dahuricae, Rhizoma Cimicifugae, Cortex Cannabis and Rhizoma Osmundae and remove impurity, dry, be placed in mortar and grind into powder, cross 200 order fine powders for subsequent use;
(2) get Herba Bidentis Bipinnatae, Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) and Herba Carpesii Cernui and smash strand juice to pieces, filter, stay juice for subsequent use;
(3) get Radix Sanguisorbae, Semen Caesalpiniae, Herba Euphorbiae Helioscopiae, Caulis Arcangelisiae, Herba Senecionis Scandentis, Herba Swertiae davidi, Herba anemones vitifoliae and Fructus glochidionis puberi(Herba Glochidii Puberi) and put into container, add distilled water to boil and extract 3 times, add first 10-15 and doubly measure distilled water immersion 4-5 hour, heated and boiled 3-4 hour, extracts; Add for the second time 8-10 and doubly measure distilled water heated and boiled 2-3 hour, extract; Add for the third time 6-8 and doubly measure distilled water heated and boiled 1~2 hour, extract; Merge three times extracting solution, filter, obtain filtrate, for subsequent use;
(4) get poinsettia and dry in the sun, slow fire parch, dries in the air cool, grinds into powder, crosses 120 order fine powders, for subsequent use;
(5) above-mentioned steps (1)-(4) prepared fine powder, juice and filtrate are mixed, add assistant medicament, utilize modern advanced pharmacy science and technology, make disinfectant solution, be described clinical laboratory's disinfectant solution.
Embodiment 2
Raw material composition and the weight of disinfectant solution effective ingredient of the present invention are:
Radix Angelicae Dahuricae 80g Herba Bidentis Bipinnatae 80g Radix Sanguisorbae 77g poinsettia 77g
Rhizoma Cimicifugae 77g Semen Caesalpiniae 70g Herba Euphorbiae Helioscopiae 70g Cortex Cannabis 68g
Caulis Arcangelisiae 68g Herba Senecionis Scandentis 68g Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) 58g Herba Swertiae davidi 58g
Rhizoma Osmundae 49g Herba Carpesii Cernui 49g Herba anemones vitifoliae 41g Fructus glochidionis puberi(Herba Glochidii Puberi) 41g.
The disinfectant solution preparation method processing step of the present embodiment is identical with embodiment 1.
Embodiment 3
Raw material composition and the weight of disinfectant solution effective ingredient of the present invention are:
Radix Angelicae Dahuricae 72g Herba Bidentis Bipinnatae 72g Radix Sanguisorbae 70g poinsettia 70g
Rhizoma Cimicifugae 70g Semen Caesalpiniae 63g Herba Euphorbiae Helioscopiae 63g Cortex Cannabis 62g
Caulis Arcangelisiae 62g Herba Senecionis Scandentis 62g Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) 52g Herba Swertiae davidi 52g
Rhizoma Osmundae 43g Herba Carpesii Cernui 43g Herba anemones vitifoliae 36g Fructus glochidionis puberi(Herba Glochidii Puberi) 36g.
The disinfectant solution preparation method processing step of the present embodiment is identical with embodiment 1.
Embodiment 4
Raw material composition and the weight of disinfectant solution effective ingredient of the present invention are:
Radix Angelicae Dahuricae 65g Herba Bidentis Bipinnatae 65g Radix Sanguisorbae 62g poinsettia 62g
Rhizoma Cimicifugae 62g Semen Caesalpiniae 56g Herba Euphorbiae Helioscopiae 56g Cortex Cannabis 55g
Caulis Arcangelisiae 55g Herba Senecionis Scandentis 55g Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) 46g Herba Swertiae davidi 46g
Rhizoma Osmundae 38g Herba Carpesii Cernui 38g Herba anemones vitifoliae 31g Fructus glochidionis puberi(Herba Glochidii Puberi) 31g.
The disinfectant solution preparation method processing step of the present embodiment is identical with embodiment 1.
Disinfectant solution of the present invention is carried out to bacteriostatic experiment below:
1, experimental strain: select three representative routine strains of laboratory, staphylococcus aureus, bacillus pyocyaneus and diphtheria corynebacterium.
2, bacterium culture medium: nutrient agar and nutrient broth medium.
3, bacteriostatic experiment divides experimental group and matched group, and experimental group is disinfectant solution of the present invention, and matched group is selected hypochlorous acid disinfectant solution.
4, specific experiment step:
(1) preparation of bacterium liquid: above-mentioned three kinds of strains are inoculated in respectively on three agar culture mediums, under 370C, cultivate 24 hours, from above-mentioned agar culture medium, picking colonies typical is inoculated in respectively in three fresh nutrient broth mediums respectively, synthermal under, cultivate 18 hours, for subsequent use.
(2) get disinfectant solution of the present invention and the each 50ml of hypochlorous acid disinfectant solution, be placed in respectively cut-back tank, it is diluted respectively to 40 times, above-mentioned two kinds of diluent solution are split respectively in three containers, be placed under aseptic condition, for subsequent use.
(3) experimental strain of above-mentioned steps (1) being supported 18 hours through nutrition broth cultivation dilutes respectively 60 times with aseptic nutrient broth, and every kind of strain is sub-packed in two culture dishs, respectively labelling experiment group and matched group.
(4) dilute solution of three parts of disinfectant solution of the present invention of above-mentioned steps (2) gained is splashed into respectively to experimental group culture dish, three parts of hypochlorous acid disinfectant solution dilute solutions splash into respectively matched group culture dish.
5, experimental observation is measured:
Above-mentioned experiment is observed, measured two groups of bacterium colony revolution mark concrete outcomes and as above show.
6, after measuring, above-mentioned culture dish is all placed under aseptic condition and leaves standstill two days, after liquid to be sterilized volatilizees completely, tracing observation is found, matched group bacterium colony continuation breeding, and experimental group bacterium colony is unchanged.
7, experimental result:
The sterilizing ability of experimental group disinfectant solution is much larger than the sterilizing ability of matched group disinfectant solution, and can suppress strain and continue breeding.

Claims (5)

1. clinical laboratory's disinfectant solution, is characterized in that: raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
Radix Angelicae Dahuricae 60-90 part Herba Bidentis Bipinnatae 60-90 part Radix Sanguisorbae 55-85 part poinsettia 55-85 part
Rhizoma Cimicifugae 55-85 part Semen Caesalpiniae 50-80 part Herba Euphorbiae Helioscopiae 50-80 part Cortex Cannabis 48-75 part
Caulis Arcangelisiae 48-75 part Herba Senecionis Scandentis 48-75 part Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) 40-66 part Herba Swertiae davidi 40-66 part
Rhizoma Osmundae 32-55 part Herba Carpesii Cernui 32-55 part Herba anemones vitifoliae 26-48 part Fructus glochidionis puberi(Herba Glochidii Puberi) 26-48 part.
2. a kind of clinical laboratory according to claim 1 disinfectant solution, is characterized in that: raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
77 parts of 77 portions of poinsettia of 80 portions of Radix Sanguisorbaes of 80 portions of Herba Bidentis Bipinnatae of the Radix Angelicae Dahuricae
68 parts of 70 parts of Cortex Cannabis of 70 parts of Herba Euphorbiae Helioscopiae of 77 parts of Semen Caesalpiniae of Rhizoma Cimicifugae
58 parts of 58 parts of Herba Swertiae davidis of 68 parts of Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) of 68 parts of Herba Senecionis Scandentiss of Caulis Arcangelisiae
41 parts of 41 parts of Fructus glochidionis puberi(Herba Glochidii Puberi)s of 49 parts of Herba anemones vitifoliaes of 49 parts of Herba Carpesii Cernui of Rhizoma Osmundae.
3. a kind of clinical laboratory according to claim 1 disinfectant solution, is characterized in that: raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
70 parts of 70 portions of poinsettia of 72 portions of Radix Sanguisorbaes of 72 portions of Herba Bidentis Bipinnatae of the Radix Angelicae Dahuricae
62 parts of 63 parts of Cortex Cannabis of 63 parts of Herba Euphorbiae Helioscopiae of 70 parts of Semen Caesalpiniae of Rhizoma Cimicifugae
52 parts of 52 parts of Herba Swertiae davidis of 62 parts of Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) of 62 parts of Herba Senecionis Scandentiss of Caulis Arcangelisiae
36 parts of 36 parts of Fructus glochidionis puberi(Herba Glochidii Puberi)s of 43 parts of Herba anemones vitifoliaes of 43 parts of Herba Carpesii Cernui of Rhizoma Osmundae.
4. a kind of clinical laboratory according to claim 1 disinfectant solution, is characterized in that: raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
62 parts of 62 portions of poinsettia of 65 portions of Radix Sanguisorbaes of 65 portions of Herba Bidentis Bipinnatae of the Radix Angelicae Dahuricae
55 parts of 56 parts of Cortex Cannabis of 56 parts of Herba Euphorbiae Helioscopiae of 62 parts of Semen Caesalpiniae of Rhizoma Cimicifugae
46 parts of 46 parts of Herba Swertiae davidis of 55 parts of Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) of 55 parts of Herba Senecionis Scandentiss of Caulis Arcangelisiae
31 parts of 31 parts of Fructus glochidionis puberi(Herba Glochidii Puberi)s of 38 parts of Herba anemones vitifoliaes of 38 parts of Herba Carpesii Cernui of Rhizoma Osmundae.
5. a preparation method for clinical laboratory's use disinfectant solution as described in claim 1-4 any one, is characterized in that: comprise following processing step:
(1) get the Radix Angelicae Dahuricae, Rhizoma Cimicifugae, Cortex Cannabis and Rhizoma Osmundae and remove impurity, dry, be placed in mortar and grind into powder, cross 200 order fine powders for subsequent use;
(2) get Herba Bidentis Bipinnatae, Rhizoma Alocasiae Cucullatae (Rhizoma Alocasiae Macrorrhizae) and Herba Carpesii Cernui and smash strand juice to pieces, filter, stay juice for subsequent use;
(3) get Radix Sanguisorbae, Semen Caesalpiniae, Herba Euphorbiae Helioscopiae, Caulis Arcangelisiae, Herba Senecionis Scandentis, Herba Swertiae davidi, Herba anemones vitifoliae and Fructus glochidionis puberi(Herba Glochidii Puberi) and put into container, add distilled water to boil and extract 3 times, add first 10-15 and doubly measure distilled water immersion 4-5 hour, heated and boiled 3-4 hour, extracts; Add for the second time 8-10 and doubly measure distilled water heated and boiled 2-3 hour, extract; Add for the third time 6-8 and doubly measure distilled water heated and boiled 1~2 hour, extract; Merge three times extracting solution, filter, obtain filtrate, for subsequent use;
(4) get poinsettia and dry in the sun, slow fire parch, dries in the air cool, grinds into powder, crosses 120 order fine powders, for subsequent use;
(5) above-mentioned steps (1)-(4) prepared fine powder, juice and filtrate are mixed, add assistant medicament, utilize modern advanced pharmacy science and technology, make disinfectant solution, be described clinical laboratory's disinfectant solution.
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104304352A (en) * 2014-10-08 2015-01-28 鞠珍红 Traditional Chinese medicament used for air disinfection of operating room and preparation method of traditional Chinese medicament
CN104644917A (en) * 2015-03-13 2015-05-27 李良学 Traditional Chinese medicine sterilization composition for department of clinical laboratory
CN106070386A (en) * 2016-06-29 2016-11-09 杨莉 The Chinese medicine liquid medicine of a kind of control laboratory sterilizing and preparation method
CN106234438A (en) * 2016-07-29 2016-12-21 杨金艳 A kind of clinical laboratory indoor disinfectant solution and preparation method

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