CN113304218B

CN113304218B - Traditional Chinese medicine for treating feline calicivirus infection

Info

Publication number: CN113304218B
Application number: CN202110683341.3A
Authority: CN
Inventors: 付本懂; 黄江妮; 伊鹏霏; 申海清
Original assignee: Jilin University
Current assignee: Jilin University
Priority date: 2021-06-21
Filing date: 2021-06-21
Publication date: 2022-05-27
Anticipated expiration: 2041-06-21
Also published as: CN113304218A

Abstract

A traditional Chinese medicine for treating feline calicivirus infection belongs to the technical field of animal medicines. The invention aims to provide the traditional Chinese medicine for treating feline calicivirus infection, which aims at the symptoms of feline calicivirus and can play a role in integrally regulating the organism of a sick animal by combining the theory of traditional Chinese medicine. The invention comprises the following effective components: indigo naturalis, catechu, gypsum, astragalus membranaceus, radix paeoniae alba, radix ophiopogonis, fried bighead atractylodes rhizome, platycodon grandiflorum, biond magnolia flower, liquorice, pummelo peel, dried orange peel, rhizoma pinellinae praeparata and almond. The invention has the function of integrally regulating the organism of the sick animals, and has the characteristics of small toxic and side effect, difficult generation of drug resistance, low sensitization and the like. Therefore, the research on the treatment effect of the indigo naturalis powder on the feline calicivirus infection has important significance for overcoming the difficult problem of the feline calicivirus treatment.

Description

Traditional Chinese medicine for treating feline calicivirus infection

Technical Field

The invention belongs to the technical field of animal medicines.

Background

Feline calicivirus infection is a systemic or respiratory disease caused by Feline Calicivirus (FCV) infection in cats. In recent years, mortality from fatal systemic diseases caused by FCV infection has been as high as 50%. The major clinical symptoms of FCV infection are upper respiratory symptoms, mouth ulcers and fever. In a few cases, FCV infection is also associated with acute claudication syndrome, abortion, enteritis and severe pneumonia.

Feline Calicivirus (FCV) is a small non-enveloped RNA virus of about 30-35nm in diameter belonging to the genus Calicivirus of the family Caliciviridae. Like other RNA viruses, the genome of FCV is constantly undergoing rapid mutations, which increases the diversity of the strains over time. However, despite the wide antigenic diversity between FCV isolates, the degree of antigenic cross-reactivity between them is sufficient to classify them as a single serotype. Feline Calicivirus (FCV) is currently prevalent worldwide, with a prevalence of 10% in domestic pet cats as measured by the european cat counseling committee, but up to 40% of cats detect FCV in a multi-cat environment, such as a hospice, cat herding, and cat hutch. Felines are the main susceptible animals, FCVs can be transmitted not only in cats, but also in lions, tigers and cheetahs.

Feline calicivirus infection has three forms of infection: acute feline calicivirus infection, chronic feline calicivirus infection, and a highly virulent systemic feline calicivirus infection. Acute or chronic FCV infection is usually caused by vaccine-sensitive FCV strains, which are not fatal. However, virulent systemic FCV infection is caused by at least two different, highly virulent, vaccine-resistant FCV strains. Acute FCV infection is clinically common, while chronic FCV infection is an unusual sequela following acute infection in cats. Highly toxic systemic FCV infections are rare and characterized by acute outbreaks that rapidly spread in environments where cats congregate in housing houses, pet hospitals, multi-cat households, and the like.

Feline calicivirus prevention is mainly achieved by vaccination, but vaccination does not produce immunity against the virus, and due to the wide antigenic variation of FCV, vaccination can only alleviate the clinical symptoms of FCV infection and cannot prevent infection, morbidity and detoxification of animals. Therefore, it is an important means to prevent FCV infection to enhance the management of breeding and to cut off the route of transmission. Keeping the environment clean and dry, ventilating well, regularly and comprehensively disinfecting, and isolating the sick cats. Meanwhile, the resistance of the cat should be strengthened, and the nutritional requirement of the cat should be ensured.

At present, no specific medicine for FCV infection exists, and the treatment means for FCV infection mainly combines symptomatic treatment and supportive treatment. Symptomatic treatment, i.e. the administration of drugs according to the corresponding clinical symptoms in sick cats, supports the application of treatment in the critical cases of feline calicivirus infections, especially in cats with stomatitis. When cats are suffering from stomatitis, they are prone to loss of appetite, and nutritional support therapy is performed via oral/nasogastric tubes or intravenous infusion. The common medicines clinically used for treating feline calicivirus infection include interferon and doxycycline, and iodine glycerol is smeared on an affected part in stomatitis cases. Broad spectrum antibiotics are also widely used to prevent secondary bacterial infections in cats following calicivirus infection. The Suno injection is an antibiotic which is widely applied to upper respiratory tract infection cases clinically, and the effective component of the Suno injection is amoxicillin potassium clavulanate and is used for infections caused by penicillin sensitive bacteria of livestock and small animals.

The previous research proves that the traditional Chinese medicine extract has good prevention and treatment effects on feline calicivirus infection, the traditional Chinese medicine is rich in various natural active substance components including terpene, steroid, alkaloid, flavonoid and the like, and some components are found to be capable of inhibiting the replication of the feline calicivirus in vitro experiments. Feline calicivirus infection has no good clinical treatment, and the commonly used interferon and antibiotic for cats have certain side effects after long-term use. The interferon can play a broad-spectrum antiviral role, but has adverse reactions of depression and easy fatigue after long-term use. And long-term use of antibiotics is easy to cause various toxic reactions, increase the drug resistance of bacteria, cause dysbacteriosis, and some animal individuals have anaphylactic reactions to the antibiotics.

Disclosure of Invention

The invention aims to provide the traditional Chinese medicine for treating feline calicivirus infection, which aims at the symptoms of feline calicivirus and can play a role in integrally regulating the organism of a sick animal by combining the theory of traditional Chinese medicine.

The invention comprises the following raw materials for preparing effective components in parts by weight:

20-40 parts of indigo naturalis, 20-40 parts of catechu, 20-40 parts of gypsum, 10-30 parts of astragalus membranaceus, 10-30 parts of radix paeoniae alba, 10-30 parts of radix ophiopogonis, 10-30 parts of fried bighead atractylodes rhizome, 5-15 parts of platycodon grandiflorum, 5-15 parts of biond magnolia flower, 5-15 parts of liquorice, 5-15 parts of exocarpium citri grandiflorum, 5-15 parts of pericarpium citri reticulatae, 5-15 parts of rhizoma pinellinae praeparata and 5-15 parts of almond.

The invention has the function of integrally regulating the organism of sick animals, and has the characteristics of small toxic and side effect, difficult generation of drug resistance, low sensitization and the like. Therefore, the research on the treatment effect of the natural indigo powder on feline calicivirus infection has important significance for overcoming the difficult problem of feline calicivirus treatment.

Detailed Description

Weighing the above Chinese medicinal materials, adding distilled water to completely submerge the Chinese medicinal materials, soaking for 30min, decocting with strong fire, boiling water, decocting with slow fire for 15 min. After the decoction, the mixture is filtered by 3 layers of gauze, the filtrate is transferred to a round-bottom flask, and the round-bottom flask is concentrated to the concentration of 1g/mL-3g/mL by a rotary evaporator.

Example 1:

20 parts of indigo naturalis, 20 parts of catechu, 20 parts of gypsum, 10 parts of astragalus membranaceus, 10 parts of radix paeoniae alba, 10 parts of radix ophiopogonis, 10 parts of fried bighead atractylodes rhizome, 5 parts of platycodon grandiflorum, 5 parts of biond magnolia flower, 5 parts of liquorice, 5 parts of exocarpium citri grandis, 5 parts of dried orange peel, 5 parts of rhizoma pinelliae praeparata and 5 parts of almond.

Example 2

30 parts of natural indigo, 30 parts of catechu, 30 parts of gypsum, 20 parts of astragalus membranaceus, 20 parts of radix paeoniae alba, 20 parts of radix ophiopogonis, 20 parts of fried bighead atractylodes rhizome, 10 parts of platycodon grandiflorum, 10 parts of biond magnolia flower, 10 parts of liquorice, 10 parts of exocarpium citri grandis, 10 parts of dried orange peel, 10 parts of rhizoma pinelliae praeparata and 10 parts of almond.

Example 3

40 parts of indigo naturalis, 40 parts of catechu, 40 parts of gypsum, 30 parts of astragalus membranaceus, 30 parts of radix paeoniae alba, 30 parts of radix ophiopogonis, 30 parts of fried bighead atractylodes rhizome, 15 parts of platycodon grandiflorum, 15 parts of biond magnolia flower, 15 parts of liquorice, 15 parts of exocarpium citri grandis, 15 parts of dried orange peel, 15 parts of rhizoma pinelliae praeparata and 15 parts of almond.

The present invention is described in detail below:

the traditional Chinese medicine compound composition comprises the following raw materials: 20-40 parts of indigo naturalis, 20-40 parts of catechu, 20-40 parts of gypsum, 10-30 parts of astragalus membranaceus, 10-30 parts of radix paeoniae alba, 10-30 parts of radix ophiopogonis, 10-30 parts of fried bighead atractylodes rhizome, 5-15 parts of platycodon grandiflorum, 5-15 parts of magnolia flower, 5-15 parts of liquorice, 5-15 parts of exocarpium citri grandiflorum, 5-15 parts of pericarpium citri reticulatae, 5-15 parts of rhizoma pinellinae praeparata and 5-15 parts of almond.

The veterinary medicine preparation comprises the following traditional Chinese medicinal materials in parts by weight: 20-30 parts of indigo naturalis, 20-30 parts of catechu, 20-30 parts of gypsum, 15-25 parts of astragalus membranaceus, 15-25 parts of radix paeoniae alba, 15-25 parts of radix ophiopogonis, 15-25 parts of fried bighead atractylodes rhizome, 5-10 parts of platycodon grandiflorum, 5-10 parts of magnolia flower, 5-10 parts of liquorice, 5-10 parts of exocarpium citri grandiflorum, 5-10 parts of pericarpium citri reticulatae, 5-10 parts of rhizoma pinellinae praeparata and 5-10 parts of almond.

The veterinary medicine preparation comprises the following traditional Chinese medicinal materials in parts by weight: 20 parts of indigo naturalis, 30 parts of catechu, 30 parts of gypsum, 20 parts of astragalus membranaceus, 20 parts of radix paeoniae alba, 20 parts of radix ophiopogonis, 20 parts of fried bighead atractylodes rhizome, 10 parts of platycodon grandiflorum, 10 parts of biond magnolia flower, 10 parts of liquorice, 10 parts of exocarpium citri grandiflorum, 10 parts of pericarpium citri reticulatae, 10 parts of rhizoma pinellinae praeparata and 10 parts of almond.

The usage and dosage of the invention are as follows:

0.5-2g drug/kg cat, 2 times a day for 7 consecutive days.

The invention also aims to provide application of the traditional Chinese medicine compound preparation in treating feline calicivirus infection.

The Chinese medicine is rich in various natural active substance components including terpene, steroid, alkaloid, flavonoid, etc., and some of the components are found to inhibit the replication of feline calicivirus in vitro experiments. Feline calicivirus infection has no good clinical treatment, and the commonly used interferon and antibiotic for cats have certain side effects after long-term use. The interferon can play a broad-spectrum antiviral role, but has adverse reactions of depression and easy fatigue after long-term use. And long-term use of antibiotics is easy to cause various toxic reactions, increase the drug resistance of bacteria, cause dysbacteriosis, and some animal individuals have anaphylactic reactions to the antibiotics. The traditional Chinese medicine treatment can play a role in integrally regulating the organism of sick animals, and has the characteristics of small toxic and side effects, difficult generation of drug resistance, low sensitization and the like.

The compound preparation of the invention is prepared by adding and subtracting indigo naturalis powder. Indigo naturalis powder, indigo naturalis in the formula is salty and cold in nature, has the effects of clearing away heat and toxic materials and cooling blood, is mainly used for treating mouth and tongue sores and is a monarch drug in the formula. The coptis and the phellodendron bark, which have the effects of clearing heat, eliminating dampness and clearing heat and removing toxicity, enter the heart channel and the kidney channel respectively to clear away heart fire and kidney fire and assist the indigo to play the effects of clearing heat, removing toxicity and eliminating dampness, are ministerial drugs. Qing Dai combined with Huang Lian and Huang Bai can remove damp-heat and move around to remove cold-heat. Herba Menthae, with pungent and cool nature and effects of dispelling pathogenic wind and heat, regulating qi-flowing and resolving food stagnation, can be used with indigo naturalis for treating wind-heat type common cold, conjunctival congestion, swelling and pain, and laryngopharynx swelling and pain. Balloonflower root, radix Platycodi, being bitter in property and neutral in flavor, enters lung meridian, has the actions of dispersing lung qi and relieving cough, and is often combined with Bo He to treat swollen and sore throat. Catechu, acts to astringe dampness and heal wound, clear lung-heat and resolve phlegm. The whole formula has the functions of combining reinforcement and elimination, and combining rigidity and softness, and has the functions of clearing away heat and toxic material, dispersing lung qi and relieving cough, eliminating phlegm and expelling pus, healing ulcer and removing necrotic tissue, and promoting granulation and astringing ulcer.

The traditional Chinese medicine components of the invention have the following medicinal properties and pharmacology:

indigo naturalis, cold in nature and bitter in taste, enters liver meridian, has the effects of clearing heat and detoxicating, cooling blood and stopping bleeding, clearing lung and relieving cough, calming liver and arresting convulsion and the like, and is used for treating epidemic febrile disease with excessive heat, blood heat hematemesis and epistaxis; sores and swelling; bite by snake and insect. Modern pharmacological research proves that the natural indigo has the effects of resisting inflammation, bacteria, viruses, tumors, immunity, pain and the like.

Catechu, slightly cold in nature, bitter and astringent in taste, enters lung and heart meridians. Has effects of promoting blood circulation, relieving pain, clearing lung-heat and eliminating phlegm. Can be used for treating traumatic injury, traumatic hemorrhage, hematemesis, epistaxis, and cough due to lung heat. Modern pharmacological studies show that the infantile tea can be used for treating diseases such as tumor resistance, virus resistance, blood fat reduction and the like.

Gypsum, Gypsum Fibrosum, with its nature cold, pungent and sweet flavor, enters lung and stomach meridians. Gypsum Fibrosum has effects of clearing heat-fire, astringing and promoting granulation, and can be used for treating exogenous febrile disease, hyperpyrexia polydipsia, lung heat cough and asthma, etc. Modern pharmacological research shows that the gypsum has the functions of clearing heat, relieving pain and resisting inflammation, and the gypsum and the compound preparation thereof can be used for defervescence, influenza treatment and the like clinically.

Huang Qi is slightly warm in nature and sweet in flavor, entering spleen and lung meridians. Has the effects of invigorating spleen and replenishing qi, consolidating superficial resistance, arresting sweating, inducing diuresis, relieving swelling, promoting sore and expelling pus. Pharmacological research proves that astragalus has the functions of enhancing immunologic function, resisting aging, bacteria and viruses and tumors and also has the protection function on heart and cerebral vessels, liver and urinary system.

Bai Shao is slightly cold in nature, bitter and sour in flavor and enters liver and spleen meridians. Has effects in nourishing blood, astringing yin, nourishing liver, and relieving pain. It is often used to treat fetal blood deficiency, spontaneous perspiration, night sweat, abdominal cramps and pains. Research shows that the white peony root has the functions of resisting inflammation, regulating immunity, resisting rheumatoid arthritis and protecting endothelial cells. The main components of the product comprise compounds such as monoterpene, glycosides, flavonoids, polysaccharides, etc., wherein the main effective component is paeoniflorin.

Radix Ophiopogonis is slightly cold in nature, sweet and slightly bitter in taste, and has effects of moistening lung, relieving cough, nourishing stomach and promoting fluid production. Mai Dong can be used for dry cough due to lung heat, dry mouth and tongue or fluid impairment due to fever, thirst and thirst, and Mai Dong also has the actions of clearing heart-fire and nourishing heart.

Bai Zhu is warm in nature, sweet and bitter in flavor, entering spleen and stomach meridians. Has the effects of invigorating spleen and replenishing qi, eliminating dampness and promoting diuresis, and consolidating superficial resistance and arresting sweating. The "medical enlightenment" records: the traditional Chinese medicine composition has the effects of removing dampness and benefiting dryness, regulating the middle warmer and replenishing qi, warming the middle warmer, removing dampness in the spleen and stomach, removing stomach heat, strengthening the spleen and stomach, promoting appetite, quenching thirst and preventing miscarriage. Modern pharmacological research proves that the bighead atractylodes rhizome has the effects of protecting the liver, regulating gastrointestinal motility, resisting inflammation and tumors, regulating the immune system, reducing blood sugar, regulating immune metabolism and the like.

Platycodon grandiflorum, neutral in nature, bitter and pungent in flavor, enters lung meridian. Has effects of dispersing lung qi, relieving cough, clearing heat from throat, relieving sore throat, eliminating phlegm, and expelling pus. Can be used for treating cough with excessive phlegm, sore throat, lung carbuncle, suppuration, chest distress, hypochondriac pain, dysentery, abdominal pain, and dysuria. Modern researches show that platycodon grandiflorum has wide pharmacological activities of eliminating phlegm, relieving cough, resisting inflammation and tumor, improving human immunity and the like.

Xin Yi, with warm nature and pungent flavor, enters lung and stomach meridians. Has effects of relieving stuffy nose and dispelling pathogenic wind and cold. Xinyi can dispel wind evil in lung to relieve nasal obstruction, is the essential herb for treating nasal disease, and can also be used for treating common cold due to wind-cold with rhinorrhea. Modern pharmacological studies prove that the magnolia flower not only has the effects of resisting inflammation, bacteria, allergy, oxidation and pain, but also can shrink nasal mucosa blood vessels and protect alcoholic liver injury.

Licorice root, radix Glycyrrhizae is neutral in nature and sweet in flavor, and enters heart, lung, spleen and stomach meridians. Has the effects of invigorating spleen and replenishing qi, moistening lung for arresting cough, clearing away heat and toxic materials, and harmonizing drug property. It is indicated for spleen and stomach deficiency, lassitude and hypodynamia, palpitation and shortness of breath, cough with profuse sputum, spasm and pain of abdomen and limbs, carbuncle and sore, and to relieve toxicity and strong action of drugs. The Chinese medicine licorice has wide pharmacological action, such as antitumor, antiphlogistic, bactericidal, antiviral, liver protecting, myocardial ischemia resisting, etc.

Hua Ju hong, warm in nature, pungent and bitter in flavor, enters lung and spleen meridians. Has the effects of regulating qi, relieving epigastric distention, eliminating dampness and resolving phlegm. It is mainly used for treating cough with excessive phlegm, food stagnation, alcohol impairment, nausea, and oppression. Pharmacological experiments prove that the Chinese medicinal composition has the remarkable effects of reducing phlegm and relieving cough, resisting inflammation and oxidation, regulating immunity, preventing and treating diabetic myocardial function injury and the like.

Tangerine peel, pericarpium Citri Reticulatae is warm in nature, bitter and pungent in flavor, and enters spleen and lung meridians. Has the effects of regulating qi, strengthening spleen, eliminating dampness and phlegm. It is indicated for abdominal fullness and pain, anorexia, emesis, cough with excessive phlegm, etc. Modern pharmacological studies prove that the dried orange peel has the effects of inhibiting gastrointestinal smooth muscle, promoting secretion of digestive juice, relaxing bronchial smooth muscle, benefiting gallbladder and resisting shock.

Fa ban Xia is warm in nature and pungent in flavor, entering spleen, stomach and lung meridians. Has effects of eliminating dampness and phlegm, and can be used for treating excessive phlegm cough and asthma, phlegm and fluid retention dizziness and palpitation, wind phlegm vertigo, phlegm syncope and headache. Modern pharmacological studies show that pinellia ternata has various pharmacological activities, mainly including vomit prevention, peptic ulcer resistance, cough and asthma relief, atherosclerosis resistance, blood pressure reduction, epilepsy resistance, aging resistance, tumor resistance, novel coronavirus pneumonia resistance and the like.

Apricot kernel, semen Armeniacae amarum, slightly warm in nature, bitter, pungent and salty in flavor, enters lung, spleen, stomach and large intestine meridians. Has effects of eliminating phlegm, relieving asthma, lowering adverse qi, and relieving vomit. Pharmacological experiment results show that the amygdalin and its degradation product have the effects of resisting atherosclerosis, resisting renal interstitial fibrosis, resisting pulmonary fibrosis, resisting hyperoxia induced lung injury, suppressing immunity, regulating immunity, resisting tumor, resisting inflammation, etc.

The invention has the advantages of precise dosage of each medicinal material, full play of the function of each component and maximum play of the drug effect by the compatibility of the medicinal materials.

The invention is used for clinical experiments. The specific method comprises the following steps:

example one:

(1) preparation of medicinal solutions

Weighing 20g of indigo naturalis, 30g of catechu, 30g of gypsum, 20g of astragalus membranaceus, 20g of radix paeoniae alba, 20g of radix ophiopogonis, 20g of fried bighead atractylodes rhizome, 10g of platycodon grandiflorum, 10g of biond magnolia flower, 10g of liquorice, 10g of exocarpium citri grandiflorum, 10g of pericarpium citri reticulatae, 10g of rhizoma pinellinae praeparata and 10g of almond. Adding distilled water, soaking for 30min, decocting with strong fire, boiling, decocting with slow fire for 15 min. After the decoction, the mixture is filtered by 3 layers of gauze, the filtrate is transferred to a round-bottom flask, and the round-bottom flask is concentrated to the concentration of 1g/mL-3g/mL by a rotary evaporator.

(2) Preparation of virus liquid

The frozen F81 cells are recovered to a T25 cell bottle, and after the cells grow to be full of monolayer, FCV CH-JL2 strain is inoculated for culturing for 20 h. Taking out the cell culture bottle, sealing with a sealing film, placing in a refrigerator of-80 ℃, and freeze thawing once. Collecting liquid in the cell bottle, centrifuging at 3000 rpm and 4 ℃ for 30min, and taking and storing supernatant. Diluting virus liquid according to gradient, inoculating to F81 cell cultured in 96-well plate, culturing for 20 h, and calculating TCID by Reed-Muench formula₅₀The virus titer of the virus liquid was measured to be 10^9.67TCID₅₀/mL。

(3) Establishment of feline calicivirus infection model and drug effect test

After 23 adult cats with the age of 6-12 months are separately fed for 7 days, the adult cats are randomly divided into 6 groups, namely a blank group, a model group, a 2 g/kg high-dose treatment group, a 1 g/kg medium-dose treatment group and a 0.5 g/kg low-dose treatment group. All the indigo naturalis powder treatment groups are treated by combining with the antibiotic Suno injection, and the drug treatment method of cats in each test group is shown in table 1.1

。

Clinical symptom changes were recorded daily for experimental cats from inoculation of FCV to end of treatment and scored according to Table 1.2

。

Test indexes

And (3) curing: after the treatment is finished, the clinical symptom score is reduced to be less than 5% of the highest value of the clinical symptom score before and during the treatment or no clinical symptom is generated, and the treatment is finished.

The effect is shown: after the treatment is finished, the clinical symptom score is reduced to be less than 30% of the highest value of the clinical symptom score before and during the treatment, and the obvious effect is achieved.

The method has the following advantages: after treatment is finished, the clinical symptom score is reduced to be less than 70% of the highest value of the clinical symptom score before and during treatment, and the effect is obtained.

And (4) invalidation: after the treatment is finished, the three standards are not met, or the clinical symptoms are not improved, or even the disease condition is aggravated, the effect is invalid.

Wherein the total effective rate is obtained by adding the cure rate, the obvious effect rate and the effective rate.

The pathological section change of the tonsil of the experimental cat is scored according to four grades, and the result is shown in table 1.3

。

(4) Clinical efficacy of traditional Chinese medicine compound on feline calicivirus infected cats

The specific results of the clinical efficacy of the groups of the artificially infected feline calicivirus are shown in Table 1.4. The traditional Chinese medicine compound combined with antibiotics developed by the invention can obviously reduce the clinical symptom score of the diseased cat, and the tonsil pathology scores of all treatment groups are smaller than those of the model group (table 1.5). 1 g/kg of traditional Chinese medicine compound group can obviously increase the tonsil IFN-gamma expression level of the diseased cat (figure 1)

。

Example two:

(1) preparation of medicinal solutions

(2) Clinical cases

8 sick cats with feline calicivirus infection detected in pet hospitals and cat houses were collected and sequenced.

Cat No. 1: white tiger, feline calicivirus and feline herpesvirus mixed infection, american kitten, aged about 12 months. The symptoms are manifested by purulent secretion of eyes, purulent secretion of nose, ulcerated bleeding of nasal septum, sneezing and cough.

Cat No. 2: dill, feline calicivirus infection, Calif., 3 months of age. The symptoms are large area ulcers of the tongue, purulent discharge of the eyes, purulent discharge of the nose, sneezing and coughing.

Cat No. 3: sharp tip, feline calicivirus infection, Zhonghua garden cat, aged about 1 year, with two ulcerated areas on the tongue, purulent nasal discharge, sneezing.

Cat No. 4: snacks, feline calicivirus and feline herpesvirus mixed infections, panda of China, age 1 or so, mucopurulent discharge, purulent discharge, mucocutaneous adhesions, sneezing, coughing, loss of appetite, and lassitude.

Cat No. 5: blue gray, feline calicivirus and feline herpesvirus co-infection, british shorthair cats aged 2 years and with symptoms of sneezing, coughing.

Cat No. 6: the symptoms of the combined infection of the Jiafei, the feline calicivirus and the feline herpesvirus, and the symptoms of the Galfei cat are eye purulent secretion, nasal purulent secretion, eyelid adhesion and nasal septum ulceration after about 6 months of age.

Cat No. 7: seventhly, mixed infection of feline calicivirus and feline herpesvirus, British shorthair cat, age 5 months, with symptoms of eye purulent secretion, nasal purulent secretion, sneezing, and cough.

Cat No. 8: the second, feline calicivirus and feline herpesvirus mixed infection, in chinese garden cats, half and half years of age, with symptoms of sneezing and coughing.

(3) Test of drug efficacy

8 cats were treated with 1 g/kg of indigo naturalis and antibiotics separately for 7 days. Clinical symptom changes were recorded daily for the experimental cats from inoculation of FCV to the end of treatment and scored according to table 1.2.

Test indexes

Wherein the total effective rate is obtained by adding the curative ratio, the effective ratio and the effective ratio.

(4) Treatment effect of traditional Chinese medicine compound on clinical cases of feline calicivirus infection

After treatment, the clinical symptom score of most cats is reduced, the symptoms of cat No. 4 are not improved (table 2.1), and the effective rate is calculated to be 87.5% (table 2.2). The normal range of leukocyte numbers is 5.5-19.5 (10)⁹L). As shown in table 2.3, WBCs at 2, 4, 5, and 8 were higher than normal, and leukocyte counts at 1, 2, 5, 6, and 8 were significantly reduced with the reduction of clinical symptoms during treatment. The leukocyte numbers did not change significantly in the remaining 3 cats (No. 4).

TABLE 2.1 clinical symptom scores of clinical cases

。

TABLE 2.2 therapeutic Effect

。

TABLE 2.3 leukocyte values (10)⁹/l）

。

Claims

1. A traditional Chinese medicine for treating feline calicivirus infection is characterized in that: the weight portions of the raw materials for preparing the effective components are as follows:

20-40 parts of indigo naturalis, 20-40 parts of catechu, 20-40 parts of gypsum, 10-30 parts of astragalus membranaceus, 10-30 parts of radix paeoniae alba, 10-30 parts of radix ophiopogonis, and,

10-30 parts of fried bighead atractylodes rhizome, 5-15 parts of platycodon grandiflorum, 5-15 parts of biond magnolia flower, 5-15 parts of liquorice, 5-15 parts of exocarpium citri grandiflorum, 5-15 parts of dried orange peel,

5-15 parts of rhizoma pinelliae praeparata and 5-15 parts of almond.