CN114431091A - Rapid propagation method of polygonatum sibiricum - Google Patents
Rapid propagation method of polygonatum sibiricum Download PDFInfo
- Publication number
- CN114431091A CN114431091A CN202210042761.8A CN202210042761A CN114431091A CN 114431091 A CN114431091 A CN 114431091A CN 202210042761 A CN202210042761 A CN 202210042761A CN 114431091 A CN114431091 A CN 114431091A
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- seeds
- taking out
- shade
- drying
- putting
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 241000037831 Polygonatum sibiricum Species 0.000 title claims abstract description 12
- 241000196324 Embryophyta Species 0.000 claims abstract description 21
- 230000035784 germination Effects 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 230000001902 propagating effect Effects 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- 230000001939 inductive effect Effects 0.000 claims abstract description 7
- 230000001678 irradiating effect Effects 0.000 claims abstract description 4
- 238000005507 spraying Methods 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 229930191978 Gibberellin Natural products 0.000 claims description 6
- 230000005059 dormancy Effects 0.000 claims description 6
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 6
- 239000003448 gibberellin Substances 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 241000238631 Hexapoda Species 0.000 claims description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 3
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000004062 cytokinin Substances 0.000 claims description 3
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
- 229910052698 phosphorus Inorganic materials 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 238000005057 refrigeration Methods 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 229960002523 mercuric chloride Drugs 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 2
- 241000756943 Codonopsis Species 0.000 description 1
- 241000756042 Polygonatum Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for rapidly propagating polygonatum sibiricum, which comprises the following steps: the method comprises the following steps: adopting mature and plump seeds from the reserved plants in the seed collection treatment, soaking the seeds in water for cleaning, and taking out the seeds; step two: after-ripening, putting seeds of rhizoma Polygonati in an incubator, spraying after-ripening solution, irradiating with ultraviolet rays for 20 min, standing for 1 hr, soaking in 20 deg.C water for 4 days, and taking out; step three: drying in the shade place, namely putting the seeds taken out in the step two in a shade place for drying in the shade or putting the seeds in a freeze dryer for drying; step four: compared with the prior art, the invention has the advantages of inducing the seeds to sprout in a short period: the increment rate is higher, and the germination and rooting rate is higher, so that the seedling raising period is greatly shortened, the seedling raising cost is reduced, and large-area propagation is facilitated.
Description
Technical Field
The invention relates to the technical field of rapid propagation of polygonatum sibiricum, in particular to a rapid propagation method of polygonatum sibiricum.
Background
Rhizoma polygonati is also named: rhizoma Polygonati, herba Elsholtziae Pendulifoliae, rhizoma Gynurae Divaricatae, rhizoma Zingiberis recens, and radix Codonopsis Lanceolatae. Is a plant of Polygonatum with transverse rhizome, cylindrical shape and enlarged nodule. The impeller is raw and has no handle. The medicinal plant has the functions of invigorating spleen, moistening lung and promoting fluid production, and the propagation mode is rhizome propagation and seed propagation.
However, the conventional seed propagation has low emergence rate, long seedling period and high seedling cost, and is unfavorable for large-area propagation of polygonatum sibiricum, so that a new method for rapidly propagating polygonatum sibiricum is urgently needed to solve the problems.
Disclosure of Invention
The invention aims to overcome the technical defects and provide a rapid polygonatum sibiricum propagation method which has high value-added rate and high germination and rooting rate and greatly shortens the seedling raising period, thereby reducing the seedling raising cost and being beneficial to large-area propagation.
In order to solve the technical problems, the technical scheme provided by the invention is as follows: a method for rapidly propagating rhizoma polygonati comprises the following steps:
the method comprises the following steps: seed collection treatment
Collecting mature and plump seeds from the reserved plants, soaking the seeds in water for cleaning, and taking out the seeds;
step two: after-ripening is carried out
Putting rhizoma Polygonati seeds in an incubator, spraying an after-ripening solution, irradiating with ultraviolet rays for 15-25 min, taking out, standing for 0.5-1.5 hr, soaking in 15-25 deg.C water for 3-5 days, and taking out;
step three: drying in the shade
Putting the seeds taken out in the step two in a shade place for drying in the shade or putting the seeds in a freeze dryer for drying;
step four: inducing the seeds to sprout in a short time
(1) Carrying out wet sand storage or cold storage treatment on the dried seeds at the temperature of 3-5 ℃ to break the biological dormancy of the seeds;
(2) directly taking out the seeds from a refrigeration house, grinding the seeds through seed coats, then sterilizing, soaking the seeds in gibberellin and a Yifuyuan germination accelerating rooting agent after sterilization, and accelerating germination at the constant temperature of 20-30 ℃;
(3) accelerating germination of seeds for about 55-65 days, growing primary rhizomes, treating the primary rhizomes with 6-BA cytokinin and gibberellin, breaking dormancy of seed balls, and inducing seedling emergence.
Preferably, in the first step, when the plants bud, the plants which grow robustly and have no plant diseases and insect pests are selected as the seed reserving plants, the phosphorus and potassium fertilizer is applied to the seed reserving plants, the seeds can be promoted to be full, the seeds are not required to be damaged during cleaning, then the outer skins and impurities are filtered, and the seeds are taken out.
Preferably, the seeds dried in the shade in the third step are prevented from being sun-dried, so that the water loss is reduced, and the germination rate is improved.
Preferably, the disinfection treatment mode in the fourth step is as follows: 2 percent sodium hypochlorite is adopted for disinfection for 15min, and then 0.1 percent mercury bichloride is adopted for disinfection for 14min, so that the disinfection pollution rate is low.
Compared with the prior art, the invention has the advantages that: the treated seeds have good growth vigor, large and fresh green leaves, large bulbs, high proliferation rate, less yellow leaves and high germination and rooting rate, greatly shorten the seedling raising period, thereby reducing the seedling raising cost and being beneficial to large-area propagation.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention discloses a method for rapidly propagating polygonatum sibiricum, which comprises the following steps:
the method comprises the following steps: seed collection treatment
Collecting mature and plump seeds from the reserved plants, soaking the seeds in water for cleaning, and taking out the seeds;
step two: after-ripening is carried out
Putting rhizoma Polygonati seeds in an incubator, spraying an after-ripening solution, irradiating with ultraviolet rays for 20 min, standing for 1 hr, soaking in 20 deg.C water for 4 days, and taking out;
step three: drying in the shade
Putting the seeds taken out in the step two in a shade place for drying in the shade or putting the seeds in a freeze dryer for drying;
step four: inducing the seeds to sprout in a short time
(1) Carrying out wet sand storage or cold storage treatment at 4 ℃ on the dried seeds to break the biological dormancy of the seeds;
(2) directly taking out the seeds from a refrigeration house, grinding the seeds through seed coats, then sterilizing, soaking the seeds in gibberellin and a Yifuyuan germination accelerating and rooting agent after sterilization, and accelerating germination at the constant temperature of 25 ℃;
(3) accelerating germination of seeds for about 60 days, growing primary rhizomes, treating the primary rhizomes by using 6-BA cytokinin and gibberellin, breaking seedball dormancy, and inducing seedling emergence.
When the plants bud, selecting the plants which grow robustly and have no plant diseases and insect pests as the plant seeds, applying phosphorus and potassium fertilizer to the plant seeds, cleaning without damaging the seeds, filtering the outer skins and impurities, and taking out the seeds.
In the third step, the seeds dried in the shade are prevented from being planted in the sun.
The fourth step is a disinfection treatment mode: sterilizing with 2% sodium hypochlorite for 15min, and sterilizing with 0.1% mercuric chloride for 14 min.
The treated seeds have good growth vigor, large and fresh green leaves, large bulbs, high proliferation rate, less yellow leaves and high germination and rooting rate, greatly shorten the seedling raising period, thereby reducing the seedling raising cost and being beneficial to large-area propagation.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (4)
1. A method for rapidly propagating rhizoma polygonati is characterized by comprising the following steps: the method comprises the following steps:
the method comprises the following steps: seed collection treatment
Collecting mature and plump seeds from the reserved plants, soaking the seeds in water for cleaning, and taking out the seeds;
step two: after-ripening is carried out
Putting rhizoma Polygonati seeds in an incubator, spraying an after-ripening solution, irradiating with ultraviolet rays for 15-25 min, taking out, standing for 0.5-1.5 hr, soaking in 15-25 deg.C water for 3-5 days, and taking out;
step three: drying in the shade
Putting the seeds taken out in the step two in a shade place for drying in the shade or putting the seeds in a freeze dryer for drying;
step four: inducing the seeds to sprout in a short time
(1) Carrying out wet sand storage or cold storage treatment on the dried seeds at the temperature of 3-5 ℃ to break the biological dormancy of the seeds;
(2) directly taking out the seeds from a refrigeration house, grinding the seeds through seed coats, then sterilizing, soaking the seeds in gibberellin and a Yifuyuan germination accelerating rooting agent after sterilization, and accelerating germination at the constant temperature of 20-30 ℃;
(3) accelerating germination of seeds for about 55-65 days, growing primary rhizomes, treating the primary rhizomes with 6-BA cytokinin and gibberellin, breaking dormancy of seed balls, and inducing seedling emergence.
2. The method for rapidly propagating polygonatum sibiricum according to claim 1, which is characterized in that: when the plants bud, selecting the plants which grow robustly and have no plant diseases and insect pests as the plant seeds, applying phosphorus and potassium fertilizer to the plant seeds, cleaning without damaging the seeds, filtering the outer skins and impurities, and taking out the seeds.
3. The method for rapidly propagating polygonatum sibiricum according to claim 1, which is characterized in that: in the third step, the seeds dried in the shade are prevented from being planted in the sun.
4. The method for rapidly propagating polygonatum sibiricum according to claim 1, which is characterized in that: the fourth step is a disinfection treatment mode: sterilizing with 2% sodium hypochlorite for 15min, and sterilizing with 0.1% mercuric chloride for 14 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210042761.8A CN114431091A (en) | 2022-01-14 | 2022-01-14 | Rapid propagation method of polygonatum sibiricum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210042761.8A CN114431091A (en) | 2022-01-14 | 2022-01-14 | Rapid propagation method of polygonatum sibiricum |
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| Publication Number | Publication Date |
|---|---|
| CN114431091A true CN114431091A (en) | 2022-05-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210042761.8A Pending CN114431091A (en) | 2022-01-14 | 2022-01-14 | Rapid propagation method of polygonatum sibiricum |
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| Country | Link |
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| CN (1) | CN114431091A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115067159A (en) * | 2022-08-03 | 2022-09-20 | 宁波久久润生物科技有限公司 | Efficient and rapid germination breeding method for polygonatum sibiricum seeds |
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| CN105660219A (en) * | 2016-01-20 | 2016-06-15 | 贵州大学 | Polygonatum cyrtonema seed rapid emerging method |
| CN107690869A (en) * | 2017-09-26 | 2018-02-16 | 界首市从生种植专业合作社 | A kind of storage practice for strengthening sealwort germination capacity of seeds |
| CN109496708A (en) * | 2018-12-19 | 2019-03-22 | 江西省鑫隆农业发展有限公司 | A kind of polygonatum cyrtonema seedling breeding method |
| CN113906860A (en) * | 2021-11-15 | 2022-01-11 | 怀化市金泉生态农业发展有限公司 | Sealwort is planted with soaking kind of a device |
-
2022
- 2022-01-14 CN CN202210042761.8A patent/CN114431091A/en active Pending
Patent Citations (4)
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|---|---|---|---|---|
| CN105660219A (en) * | 2016-01-20 | 2016-06-15 | 贵州大学 | Polygonatum cyrtonema seed rapid emerging method |
| CN107690869A (en) * | 2017-09-26 | 2018-02-16 | 界首市从生种植专业合作社 | A kind of storage practice for strengthening sealwort germination capacity of seeds |
| CN109496708A (en) * | 2018-12-19 | 2019-03-22 | 江西省鑫隆农业发展有限公司 | A kind of polygonatum cyrtonema seedling breeding method |
| CN113906860A (en) * | 2021-11-15 | 2022-01-11 | 怀化市金泉生态农业发展有限公司 | Sealwort is planted with soaking kind of a device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115067159A (en) * | 2022-08-03 | 2022-09-20 | 宁波久久润生物科技有限公司 | Efficient and rapid germination breeding method for polygonatum sibiricum seeds |
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Application publication date: 20220506 |
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