CN114403164A - Traditional Chinese medicine composition for preventing and treating polygonatum root rot and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating polygonatum root rot and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The invention discloses a traditional Chinese medicine composition for preventing and treating polygonatum root rot, a preparation method and application thereof, wherein the traditional Chinese medicine composition comprises phellodendron bark, a golden thread extract and sterile water according to a certain proportion, and the steps are as follows: (1) weighing cortex Phellodendri and rhizoma Coptidis, pouring into a round bottle with an electric heating jacket, and extracting cortex Phellodendri and rhizoma Coptidis to obtain extractive solution. (2) And (4) carrying out suction filtration on the extracting solution, pouring alcohol, and extracting to obtain the extracting solution. (3) And (4) carrying out suction filtration on the extracting solution, pouring the extracting solution into a round bottle of a rotary evaporator, and carrying out rotary evaporation under reduced pressure to obtain the extracts of the golden cypress and the golden thread. (4) Pouring the obtained Chinese medicinal composition into a bottle, and storing. (5) Putting cortex Phellodendri extract and rhizoma Smilacis Glabrae extract into a container at a certain ratio, and stirring to obtain the Chinese medicinal composition. (6) The composition has strong antibacterial effect on the root rot of the polygonatum odoratum, has little environmental pollution and no residual toxicity, effectively avoids the harm of chemical pesticides and greatly reduces the possibility of drug resistance of pathogenic bacteria.
Description
The application is a divisional application of a traditional Chinese medicine composition for preventing and treating polygonatum root rot, a preparation method and an application, wherein the application date is 2016, 11 and 22, and the application number is 201611047104.3.
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a traditional Chinese medicine composition for preventing and treating polygonatum odoratum root rot, a preparation method of the traditional Chinese medicine composition for preventing and treating polygonatum odoratum root rot, and application of the traditional Chinese medicine composition for preventing and treating polygonatum odoratum root rot.
Background
Polygonatum odoratum (Mill.) Druceflo is a Liliaea plant of Liliaceae, namely rhizoma Polygonati Odorati and rhizoma Polygonati Odorati, has effects of promoting fluid production to quench thirst, nourishing yin and moistening dryness, and is a commercial crop with fleshy rhizome which can be used as both medicine and food. In the production and reproduction processes of polygonatum odoratum, a large number of diseases are caused, the most serious is polygonatum odoratum root rot, and the main pathogeny of polygonatum odoratum root rot is Fusarium (Fusarium redolen). The disease has a tendency of gradually increasing diffusion in recent years, and the occurrence and spread of polygonatum root rot affect the yield and quality of polygonatum.
The rhizoctonia rot of the polygonatum is a soil-borne disease, and is mainly prevented and controlled by chemical reagents such as carbendazim, chlorothalonil and the like at present. With the use of a large amount of chemical pesticides, on the one hand, the drug resistance of pathogenic bacteria is caused; on the other hand, the problems of pesticide residue, excessive heavy metal and the like are caused and are more and more resisted by consumers. Therefore, the development of a pollution-free, green and environment-friendly biopesticide for preventing and treating the rhizopus of the polygonatum becomes the key for solving the problems.
According to research reports, many traditional Chinese medicines have been used for preventing and treating diseases. Such as honeysuckle, stemona root, clove and the like, which are used for inhibiting pathogenic bacteria such as anthracnose, pedicellus et pericarpium citri reticulatae and the like; bupleuri radix, fructus Gardeniae, herba Menthae, Poria, etc. can inhibit Staphylococcus aureus; the plantain herb has better bacteriostatic effect on apple ring spot and citrus anthracnose; the Chinese gall has an obvious bacteriostatic effect on the black spot of the Chinese cabbage; the asarum has a good bacteriostatic effect on cotton fusarium wilt. The application of the Chinese herbal medicine in preventing and treating the polygonatum root rot is not reported.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine composition for preventing and treating polygonatum odoratum root rot, which is an environment-friendly traditional Chinese medicine composition with the advantages of reasonable formula, convenience in use, no pollution, greenness, safety and difficulty in generating drug resistance, and the composition of the golden thread and the golden cypress has a better antibacterial effect on the polygonatum odoratum root rot and is stronger than a single traditional Chinese medicine microbial inoculum. The traditional Chinese medicine composition has the characteristics of strong antibacterial effect, small environmental pollution, no residual toxicity and the like. Effectively avoiding the harm of chemical pesticide. The Chinese medicinal material composition greatly reduces the possibility of drug resistance of pathogenic bacteria.
The invention also aims to provide a preparation method of the traditional Chinese medicine composition for preventing and treating polygonatum root rot, which is easy to implement and simple and convenient to operate. The composition for preventing and treating the polygonatum root rot is prepared from golden thread and golden cypress, and has the advantages of convenient raw material acquisition and low price. The extraction process is simple and easy to implement, and the extraction process can be completed by common distillation equipment without expensive test instruments. The production process has strong controllability and good quality reproducibility.
The invention also aims to provide application of the traditional Chinese medicine composition in preparing a medicine for treating or preventing polygonatum root rot. The developed traditional Chinese medicine composition of the golden cypress and the golden thread has obvious bacteriostatic effect on the polygonatum root rot, can better prevent and control the occurrence and spread of the polygonatum root rot, and reduces the economic loss of farmers and growers. Good control effect, long control time, and reduced environmental pollution and drug resistance to diseases and insect pests caused by chemical pesticide. The operation process is simple and easy to implement, professional basic knowledge is not needed, and the method has basic popularization value.
In order to achieve the purpose, the invention adopts the following technical measures:
a traditional Chinese medicine composition for preventing and treating polygonatum root rot is composed of the following raw materials in parts by weight:
a traditional Chinese medicine composition for preventing and treating polygonatum root rot is composed of the following raw materials in parts by weight (in a preferred range):
a traditional Chinese medicine composition for preventing and treating polygonatum root rot comprises the following raw materials in parts by weight (in a good range):
a traditional Chinese medicine composition for preventing and treating polygonatum root rot comprises the following raw materials in parts by weight (preferably in a range):
a traditional Chinese medicine composition for preventing and treating polygonatum root rot comprises the following raw materials in parts by weight (optimal value):
42 Chinese medicinal materials are selected for the experiment to carry out an antibacterial test on the polygonatum odoratum root rot, and the optimal medicament for inhibiting the polygonatum odoratum root rot is screened out. After the medicaments are combined, the optimum medicament combination of the phellodendron and the rhizoma coptidis and the optimum proportion of the phellodendron and the rhizoma coptidis are screened out.
The invention also provides a preparation method of the traditional Chinese medicine composition (paste) for preventing and treating the polygonatum root rot, which comprises the following steps:
(1) weighing cortex phellodendri and rhizoma coptidis according to a proportion, pouring into an electric heating sleeve round bottle respectively, pouring 1000mL of 75% (75mL of absolute ethyl alcohol and 25mL of water) of alcohol respectively, starting an electric heating sleeve (DRT-XS), and extracting the cortex phellodendri and the rhizoma coptidis respectively, wherein the extraction time is controlled to be 28-32 min, and the temperature is controlled to be 93-97 ℃ to obtain a first extracting solution.
(2) And respectively carrying out suction filtration on the first extracting solution, pouring 500mL of 75% (75mL of absolute ethyl alcohol and 25mL of water) alcohol, controlling the extracting time to be 28-32 min and the temperature to be 93-97 ℃ to obtain a second extracting solution.
(3) And (3) respectively carrying out suction filtration on the second extracting solution, pouring the second extracting solution into a round bottle (not exceeding the total volume 1/3) of a rotary evaporator (R2003KB), and carrying out reduced pressure rotary evaporation to obtain a paste, wherein the rotation parameters are as follows: 60-75/min; the refrigeration temperature is controlled as follows: and (4) obtaining extracts (paste) of the golden cypress and the golden thread at the temperature of-2.0-10 ℃.
(4) Respectively pouring the obtained Chinese medicinal composition (paste) into bottles, weighing the empty bottles, recording, labeling, writing serial number names, and storing in a refrigerator at 4 deg.C for use.
(5) Putting the phellodendron extract and the Coptis chinensis Franch extract into a container according to a certain proportion, stirring, uniformly mixing, and adding sterile water into the container to obtain the traditional Chinese medicine composition bacteriostatic agent.
The screening and verification process of the ratio of phellodendron amurense and golden thread to the inhibition effect of the fragrant fusarium is as follows:
1. dissolving the extracted cortex Phellodendri and rhizoma Coptidis extract in sterile water respectively (paste). Respectively shaking, dissolving, centrifuging, collecting supernatant, and sterilizing with bacterial filter.
2. Then, the sterilized golden cypress and the golden thread are taken and proportioned according to different proportions, poured into a bottle, labeled and written with serial number names.
3. And (3) punching the filter membrane with the aperture of 0.45um by using a puncher to obtain a small piece of filter paper, and sterilizing the small piece of filter paper at high temperature (121 ℃, 25-30min) for later use.
4. The fusarium graminearum f.redoles is identified as fusarium graminearum f.redoles after PCR, sequencing and NCBI comparison.
5. 200g of potato (peeled), 20g of cane sugar, 17g of agar powder and 1000mL of water are boiled for 30min, filtered and sterilized at high temperature (121 ℃, 25-30min) to prepare a culture medium for later use.
6. The aromatic fusarium f.redox was inoculated to PDA medium for activated culture and tested when the strain was cultured for about 7 days when the strain activity was optimal.
7. Cultured Fusarium F.redox with the best activity was beaten into cakes with a sterile punch of 6mm diameter.
8. Placing a small circular filter membrane at the center of the culture dish, placing one bacterial cake at the center of the filter membrane and the edge of the culture dish, and placing the other bacterial cake at the other symmetrical edge. Taking 25 mu L of the cortex phellodendri and the rhizoma coptidis composition prepared in different proportions respectively, dripping the composition on a culture medium for culture, repeating the steps for three times for each composition in different proportions, using water as a blank control, and using carbendazim as a positive control. Culturing in a constant temperature incubator at 28 ℃ for 7 d. And observing the size of the inhibition zone.
9. When the ratio of the phellodendron amurense to the rhizoma coptidis is 1:2, 1:1, 1:5, 2:1 and 5:1, the size of the inhibition zone is respectively 19.5mm, 16.75mm, 15.5mm, 14.25mm and 13mm, and the size of the inhibition zone of the blank control is 0. The 500-fold inhibition zone size of carbendazim on aromatic fusarium was 20.5mm (see table 1).
10. As can be seen from the size of the inhibition zones, the traditional Chinese medicine composition shows better inhibition effect at five ratios of 1:2, 1:1, 1:5, 2:1 and 5: 1. Especially when the ratio of the phellodendron and the rhizoma coptidis is 1:2, the size of the inhibition zone is equivalent to that of 500 times of carbendazim.
Table 1 shows the inhibition zone size of different concentration ratios of phellodendron and golden thread to Fusarium F
An application of a Chinese medicinal composition (preparation) in preparing a medicament for treating or preventing polygonatum root rot comprises the following steps:
the screened combination of the golden thread and the amur corktree bark in the optimal proportion is prepared into water aqua which can be applied to fields by methods of seed dressing and root irrigation. The composition of the golden thread and the amur corktree bark and contrast sterile water are used for carrying out a field efficacy test on the polygonatum root rot.
A. Mixing cortex Phellodendri Chinese medicinal composition (paste) and rhizoma Coptidis Chinese medicinal composition (paste) at a ratio of 83.3% and 16.7% (the ratio of cortex Phellodendri and rhizoma Coptidis is 5:1) to obtain aqua. Sterile water is added, and the volume is adjusted to 1000 parts.
B. Healthy polygonatum rhizome with the length of about 15cm is selected, soaked for 28-32 min by the traditional Chinese medicine composition and planted in a land where the polygonatum root rot is severe, and the healthy polygonatum rhizome with the length of about 15cm is soaked for 28-32 min by sterile water and planted in the same land of a control test to serve as a blank control.
C. Carrying out conventional management after the polygonatum odoratum seedlings emerge, detecting the occurrence condition of root rot within 30 days without applying any fertilizer and pesticide, pulling out and cleaning the seedlings and the rhizomes when the optimum period is reached, and detecting the quantity of disease spots of the polygonatum odoratum rhizomes.
D. After phellodendron (extract) and golden thread (extract) are mixed according to the proportion of 83.3 percent and 16.7 percent (the proportion of phellodendron and golden thread is 5:1) to prepare an aqueous solution to soak healthy polygonatum rhizome, the traditional Chinese medicine mixture has little influence on the germination rate of the polygonatum, and is equivalent to a blank control, compared with the blank control, the scab of the treated polygonatum rhizome is obviously reduced, and has obvious inhibiting effect on polygonatum root rot F.redolens, the number of the scab of the polygonatum rhizome is 5.3, while the number of the scab of the polygonatum rhizome treated by the phellodendron (extract) and the golden thread (extract) according to the proportion of 83.3 percent and 16.7 percent (5:1) is 1.5, the control effect reaches 71.7 percent (the proportion of phellodendron and golden thread is 5:1 in a field test, the indoor bacteriostatic circle of the phellodendron and the golden thread is the minimum proportion, the minimum combination proportion of the bacteriostatic circle in the field test is selected when the indoor bacteriostatic effect of the proportion is verified, the field control effect of the proportion is good, so the traditional Chinese medicine composition shows better bacteriostasis effect in five proportions of 1:2, 1:1, 1:5, 2:1 and 5:1)
E. The field test proves that the composition has better control effect on the root rot of the polygonatum odoratum.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the composition of phellodendron and golden thread is used for preventing and treating polygonatum root rot (F. redox) for the first time, and has a good bacteriostatic effect.
(2) The traditional Chinese medicine composition has the characteristics of strong antibacterial effect, small environmental pollution, no residual toxicity and the like. Effectively avoiding the harm of chemical pesticide.
(3) The Chinese medicinal material composition greatly reduces the possibility of drug resistance of pathogenic bacteria.
(4) The invention provides a preparation method of a traditional Chinese medicine composition for preventing and treating polygonatum root rot, which is easy and convenient to operate. The extraction process can be completed by ordinary distillation equipment without expensive equipment.
(5) The raw materials are low in price, the production process is high in controllability, the quality reproducibility is good, and the operability and the popularization are realized.
The data on the experiments are as follows: please refer to Table 1, which shows the inhibition zone of phellodendron bark and Coptis chinensis Franch with different ratios for Fusarium fragrans.
Drawings
Fig. 1 is a diagram of the bacteriostatic effect of sterile water on aromatic fusarium f.redox; the right graph is the bacteriostatic effect graph of the traditional Chinese medicine composition (the ratio is 1:2) of the phellodendron (extract) and the rhizoma coptidis (extract) on the aromatic fusarium f.
1. In a field test, when phellodendron amurense (extract) and golden thread (extract) are used as aqueous agents in a ratio of 83.3 percent to 16.7 percent (5:1) to soak healthy polygonatum odoratum rhizomes before planting, the prevention and treatment effect reaches 71.7 percent. Therefore, the composition has better control effect on the root rot of the polygonatum odoratum.
2. The operation process is simple and easy to implement, professional basic knowledge is not needed, and the method is suitable for basic popularization.
3. Can not cause environmental pollution and harm to users.
Detailed Description
Example 1:
a traditional Chinese medicine composition for preventing and treating polygonatum root rot is composed of the following raw materials in parts by weight:
a preparation method of a traditional Chinese medicine composition (paste) for preventing and treating polygonatum root rot comprises the following steps:
(1) weighing cortex Phellodendri and rhizoma Coptidis, respectively, pouring into round electric heating jacket bottles, respectively, pouring 1000mL of 75% (75mL of anhydrous ethanol and 25mL of water) ethanol, respectively, starting the electric heating jacket, and extracting cortex Phellodendri and rhizoma Coptidis respectively at 93 or 94 or 95 or 96 or 97 deg.C for 28 or 29 or 30 or 31 or 32min to obtain first extractive solution;
(2) respectively carrying out suction filtration on the first extracting solution, then pouring 500mL of 75% (75mL of absolute ethanol and 25mL of water) of alcohol, controlling the extracting time to be 28 or 29 or 30 or 31 or 32min, and controlling the temperature to be 93 or 94 or 95 or 96 or 97 ℃ to obtain a second extracting solution;
(3) and (3) respectively carrying out suction filtration on the second extracting solution, pouring the second extracting solution into a round bottle (not exceeding the total volume 1/3) of a rotary evaporator (R2003KB), and carrying out reduced pressure rotary evaporation to obtain a paste, wherein the rotation parameters are as follows: 60 or 63 or 66 or 69 or 72 or 75/min; the refrigeration temperature is controlled as follows: 2.0 or-1 or 3 or 5 or 8 or 10 deg.C to obtain cortex Phellodendri and rhizoma Coptidis extract (paste);
(4) respectively pouring the obtained Chinese medicinal composition (paste) into bottles, weighing the empty bottles, recording, labeling, writing serial number names, and storing in a refrigerator at 4 deg.C for use.
(5) Putting the phellodendron extract and the golden thread extract into a container according to a certain proportion, stirring, uniformly stirring and mixing, adding sterile water into the container, and fixing the volume to 1000 parts to obtain the traditional Chinese medicine composition preparation.
(6) To be used for preventing and treating the polygonatum root rot.
Can better prevent and control the occurrence and spread of the polygonatum root rot and reduce the economic loss of farmers and growers. Good control effect, long control time, and reduced environmental pollution and drug resistance to diseases and insect pests caused by chemical pesticide.
A traditional Chinese medicine composition for preventing and treating polygonatum root rot is composed of the following raw materials in parts by weight:
the preparation method is the same as that of example 1.
Example 12:
an application of a Chinese medicinal composition (preparation) in preparing a medicament for treating or preventing polygonatum root rot comprises the following steps:
A. mixing cortex Phellodendri Chinese medicinal composition (paste) and rhizoma Coptidis Chinese medicinal composition (paste) at a ratio of 83.3% and 16.7% (5:1) to obtain aqua. Sterile water is added, and the volume is adjusted to 1000 parts.
B. Healthy polygonatum rhizome with the length of about 15cm is selected to be soaked in the traditional Chinese medicine composition for 30min and then planted in a land where the polygonatum root rot is severe, and the healthy polygonatum rhizome with the length of about 15cm is soaked in sterile water for 28-32 min and then planted in the same land where a control test is carried out to serve as a blank control.
C. Carrying out conventional management after the polygonatum odoratum seedlings emerge, detecting the occurrence condition of root rot within 30 days without applying any fertilizer and pesticide, pulling out and cleaning the seedlings and the rhizomes when the optimum period is reached, and detecting the quantity of disease spots of the polygonatum odoratum rhizomes.
After phellodendron (paste or preparation) and golden thread (paste or preparation) are mixed according to the proportion of 83.3 percent and 16.7 percent (5:1) to prepare an aqueous solution for soaking healthy polygonatum rhizome, the traditional Chinese medicine mixture has little influence on the germination rate of polygonatum and is equivalent to a blank control. Compared with the blank control, the treated polygonatum odoratum rhizome scab is obviously reduced, and the polygonatum odoratum root rot is obviously inhibited. The number of the disease spots of the control polygonatum rhizome is 5.3, while the number of the disease spots of the polygonatum rhizome treated by phellodendron (paste or preparation) and native coptis (paste or preparation) according to the ratio of 83.3 percent to 16.7 percent (5:1) is 1.5, and the control effect reaches 71.7 percent.
The pathogenic bacteria are aromatic fusarium f, which causes polygonatum root rot.
The preparation method of the traditional Chinese medicine composition provided by the invention is easy to implement and simple and convenient to operate, and the obtained traditional Chinese medicine composition has the characteristics of strong antibacterial effect, small environmental pollution, no residual toxicity and the like, can better prevent and control the occurrence and spread of polygonatum root rot, and reduces the economic loss of farmers and growers. Good control effect, long control time, and reduced environmental pollution and drug resistance to diseases and insect pests caused by chemical pesticide. The operation process is simple and easy to implement, professional basic knowledge is not needed, and the method has basic popularization value.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.
Claims (8)
1. A preparation method of a traditional Chinese medicine composition for preventing and treating polygonatum root rot is characterized by comprising the following steps:
(1) weighing cortex phellodendri and rhizoma coptidis according to a ratio, pouring into an electric heating sleeve round bottle respectively, pouring 1000ml of 75% alcohol respectively, starting the electric heating sleeve, and extracting the cortex phellodendri and the rhizoma coptidis respectively, wherein the extraction time is controlled to be 28-32 min, and the temperature is controlled to be 93-97 ℃ to obtain a first extracting solution;
(2) respectively carrying out suction filtration on the primary extracting solution, pouring 500ml of 75% alcohol, controlling the extraction time to be 28-32 min and the temperature to be 93-97 ℃ to obtain a secondary extracting solution;
(3) carrying out suction filtration on the extracting solution for the second time respectively, pouring the extracting solution for the second time into a round bottle of a rotary evaporator, and carrying out reduced pressure rotary evaporation to form a paste, wherein the rotary parameters are as follows: 60-75/min; controlling the refrigeration temperature to be-2.0-10 ℃, and respectively obtaining extracts of the golden cypress and the golden thread;
(4) respectively pouring the extracts of cortex Phellodendri and rhizoma Coptidis into bottles, and storing in a refrigerator at 4 deg.C;
(5) putting the phellodendron extract and the golden thread extract into a container according to a certain proportion, stirring, uniformly stirring and mixing, and adding sterile water into the container to obtain the traditional Chinese medicine composition for preventing and treating the polygonatum root rot;
the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:
80-360 parts of phellodendron extract
70-380 parts of rhizoma coptidis extractive
500-745 parts of sterile water.
2. The preparation method of claim 1, wherein the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:
120-290 parts of phellodendron extract
100-280 parts of rhizoma coptidis extractive
550-720 parts of sterile water.
3. The preparation method of claim 1, wherein the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:
150-210 parts of phellodendron extract
130-220 parts of rhizoma coptidis extractive
580-680 parts of sterile water.
4. The preparation method of claim 1, wherein the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:
5. the preparation method of claim 1, wherein the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:
6. the traditional Chinese medicine composition for preventing and treating polygonatum root rot, which is obtained by the preparation method of any one of claims 1-5.
7. The application of the traditional Chinese medicine composition for preventing and treating polygonatum odoratum root rot obtained by the preparation method according to any one of claims 1 to 5 in preparing a medicine for treating or preventing polygonatum odoratum root rot.
8. The use according to claim 7, wherein the pathogenic bacterium causing the Polygonatum odoratum root rot is Fusarium fragrans (F.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001062089A1 (en) * | 2000-02-25 | 2001-08-30 | Michelangelo Leis | Use of piperidine benzoderivatives as fungicides |
| US20040248764A1 (en) * | 2001-08-28 | 2004-12-09 | Franklin Lanny U | Treatment and prevention of infections in plants |
| CN101617696A (en) * | 2008-07-03 | 2010-01-06 | 王庆虎 | Preparation method of plant medicament for preventing and treating cabbage aphids |
| CN101623011A (en) * | 2008-07-07 | 2010-01-13 | 朱绍刚 | Preparation method of plant medicament for preventing and treating rice blast |
| CN101843193A (en) * | 2010-05-26 | 2010-09-29 | 湖南三湘中药饮片有限公司 | GAP cultivation method of polygonatum odoratum |
| CN105076163A (en) * | 2015-08-26 | 2015-11-25 | 广西大学 | New application of berberine serving as agricultural fungicide |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041528A (en) * | 2014-06-25 | 2014-09-17 | 无锡市崇安区科技创业服务中心 | Medicament for controlling anthracnose of michelia figo (lour.) spreng and application method thereof |
| CN105638758B (en) * | 2015-05-05 | 2018-10-23 | 北京联合大学 | A kind of tea oil-compound suspension emulsion of jamaicin antibacterial and preparation method thereof |
| CN106069109A (en) * | 2016-06-30 | 2016-11-09 | 彭建平 | The method of Phyllostachys pubescens Mazei ex H.de Lebaie underwood planting Rhizoma Polygonati Odorati |
-
2016
- 2016-11-22 CN CN202210041067.4A patent/CN114403164A/en active Pending
- 2016-11-22 CN CN201611047104.3A patent/CN106719893A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001062089A1 (en) * | 2000-02-25 | 2001-08-30 | Michelangelo Leis | Use of piperidine benzoderivatives as fungicides |
| US20040248764A1 (en) * | 2001-08-28 | 2004-12-09 | Franklin Lanny U | Treatment and prevention of infections in plants |
| CN101617696A (en) * | 2008-07-03 | 2010-01-06 | 王庆虎 | Preparation method of plant medicament for preventing and treating cabbage aphids |
| CN101623011A (en) * | 2008-07-07 | 2010-01-13 | 朱绍刚 | Preparation method of plant medicament for preventing and treating rice blast |
| CN101843193A (en) * | 2010-05-26 | 2010-09-29 | 湖南三湘中药饮片有限公司 | GAP cultivation method of polygonatum odoratum |
| CN105076163A (en) * | 2015-08-26 | 2015-11-25 | 广西大学 | New application of berberine serving as agricultural fungicide |
Non-Patent Citations (5)
| Title |
|---|
| 任伟: "石蒜碱和小檗碱对植物病原真菌抑制作用及其抑菌生理指标分析", 《农业科技》 * |
| 吴金平等: "玉竹根腐病病原鉴定", 《中国植保导刊》 * |
| 崔蕾: "不同产地引种玉竹品质评价及病虫害防治试验研究", 《农业科技》 * |
| 江苏新医学院 编: "《中药大辞典 上册》", 31 January 1977, 上海人民出版社 * |
| 王俊儒 主编: "《天然产物提取分离与鉴定技术》", 31 May 2006, 西北农林科技大学出版社 * |
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